Rh2CG048700

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008825
Physical Location & Seq
Forward (+)
3877454 .. 3880410
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG048700.1

Sequence Viewer

Length: 996 bp
ATGGACCGATTTGTCGATGACGGATCTTTAGAGGATAATTTTGAATCATTCTTATCACTTGATGATGCTGACTCTAGGGGTAGAGTTGCTCGGCGTTCAGATGTCAGCAGAGGCTTCACTTTTTCGGAAGTCGGGGTTATTCCTGCAAGTTCAAGTGAAGTCGAGTGCTGTCACTTCTCAGCAGATGGGAAAACACTTGCCACTGGTGGGCATGATCGAAAGGCTGTATTGTGGTCTACAGAGTCCTTCGCTGTAAAGTCTACGCTTGAAGAGCATTCTCAGTGGATAACGGATGTTCGATTCAGTCCTAGTATGTCAAGGCTAGCTACATCTTCCGCTGACAAAACTGTCAGGGTTTGGGATGTTGATAATCCTGGTTATTCACTTCGTACTTTTATGGGGCATTCTACAACTGTTATGTCACTTGACTTCCACCCGGCTAAAGAGGATCTTCTGTGCTCTTGTGATAGCAACAGTGAGATAAGATACTGGAGTATCAAGAATGGTTGTTGTATTGGAGTTTCCAAGGGTGGTGCAACTCAGATGAGGTTTCAACCTCGTCTTGGAAGGATGCTTGCTGCTGCAGGTGATACTCTTGTATCCATAATTGATGTAGAGATCGGGTGTTGCACGATTAAATTACAGGGTCATAAGAACCTTGTCAATTCTGTTTGCTGGGATTCTTCTGGTGAGTATCTAGCCTCTGTGAGTGATGACTCGGTTAGAGTATGGGCAGTTGGCTCCAGTAGCAAATGCGAATGCCTTTACGAGTTAAGCTGTTCTGGCAACAAATTTCAGACATGCGTCTTCCATCCTACTTATCCATCATTGTTGGTAATCGGCTGTTATGAGACATTGGAACTTTGGAACATGAGCGAGAACAAGACAATGACCTTGCATGCACATGACAAGCTAGTATCTTCTTTGGCAGCGTCAAGTTCTACTGGCATGGTAGCTTCAGCTAGCCATGATAAGTGCGTGAAGCTATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

331

Amino Acids

36.25

Weight (kDa)

5.58

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 38 - 174 6.2e-13 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 41 - 244 5.9e-17 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 41 - 103 5.1e-09 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 44 - 166 6.5e-14 TEP-1 second beta-propeller
EIF3I PF24805 44 - 124 1.2e-06 EIF3I
WD40_WDHD1_1st PF24817 45 - 124 7.9e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 50 - 246 1.2e-30 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 50 - 173 2.2e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 51 - 166 2.5e-23 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 51 - 169 6.8e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 52 - 170 3.4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 53 - 151 1.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 54 - 165 2.9e-11 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 57 - 124 9.2e-08 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 83 - 172 1.6e-12 CDC20/Fizzy WD40 domain
WD40 PF00400 86 - 121 1.2e-09 WD domain, G-beta repeat
WDR55 PF24796 86 - 331 8.4e-12 WDR55
Beta-prop_EIPR1 PF23609 86 - 164 6.2e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 92 - 331 7.5e-37 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 104 - 172 1.4e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 152 - 255 1.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 177 - 330 1.6e-24 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 177 - 273 1.1e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 180 - 331 4.2e-14 WDHD1 first WD40 domain
WD40_Prp19 PF24814 180 - 330 1.4e-14 Prp19 WD40 domain
WD40_Gbeta PF25391 182 - 330 2.4e-06 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 195 - 297 2.2e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 209 - 244 3.2e-08 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 221 - 331 4.8e-09 WDR3 second beta-propeller domain
WD40 PF00400 296 - 330 3.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 575
AasI GACNNNNNNGTC 2 cut(s) 11, 347
Acc36I ACCTGC 1 cut(s) 575
AccI GTMKAC 2 cut(s) 236, 260
AciI CCGC 1 cut(s) 336
AclWI GGATC 2 cut(s) 31, 456
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 207, 563
AgsI TTSAA 4 cut(s) 44, 153, 269, 554
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 6 cut(s) 326, 777, 913, 956, 962, 985
AluI AGCT 6 cut(s) 326, 777, 913, 956, 962, 985
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 845
AlwI GGATC 2 cut(s) 31, 456
ApeKI GCWGC 3 cut(s) 578, 581, 929
ApoI RAATTY 1 cut(s) 791
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 599, 701
AsuNHI GCTAGC 2 cut(s) 322, 962
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 8 cut(s) 478, 478, 511, 511, 582, 582, 615, 615
BbsI GAAGAC 1 cut(s) 799
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 3 cut(s) 565, 568, 941
BccI CCATC 3 cut(s) 179, 819, 832
BciT130I CCWGG 1 cut(s) 375
BciVI GTATCC 1 cut(s) 610
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 845
BfaI CTAG 6 cut(s) 75, 309, 323, 698, 914, 963
BfmI CTRYAG 2 cut(s) 237, 582
BfuAI ACCTGC 1 cut(s) 575
BfuI GTATCC 1 cut(s) 610
BisI GCNGC 3 cut(s) 579, 582, 930
BlsI GCNGC 3 cut(s) 580, 583, 931
Bme1390I CCNGG 2 cut(s) 375, 437
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 742
BmrFI CCNGG 2 cut(s) 375, 437
BmsI GCATC 2 cut(s) 55, 561
BmtI GCTAGC 2 cut(s) 326, 966
BoxI GACNNNNGTC 1 cut(s) 803
BpiI GAAGAC 1 cut(s) 799
BpmI CTGGAG 2 cut(s) 511, 727
BpuMI CCSGG 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 563
Bse1I ACTGG 4 cut(s) 208, 494, 744, 949
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 4 cut(s) 298, 367, 576, 811
BseLI CCNNNNNNNGG 2 cut(s) 207, 563
BseMII CTCAG 3 cut(s) 192, 293, 554
BseNI ACTGG 4 cut(s) 208, 494, 744, 949
BseXI GCAGC 3 cut(s) 565, 568, 941
BseYI CCCAGC 1 cut(s) 675
BsiHKAI GWGCWC 1 cut(s) 461
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 207, 563
BsmAI GTCTC 1 cut(s) 845
BsmI GAATGC 3 cut(s) 274, 403, 764
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 4 cut(s) 23, 214, 448, 618
BspACI CCGC 1 cut(s) 336
BspCNI CTCAG 3 cut(s) 191, 292, 553
BspLI GGNNCC 1 cut(s) 742
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 1 cut(s) 575
BspOI GCTAGC 2 cut(s) 326, 966
BspPI GGATC 2 cut(s) 31, 456
BspQI GCTCTTC 1 cut(s) 264
BsrI ACTGG 4 cut(s) 208, 494, 744, 949
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 4 cut(s) 23, 214, 448, 618
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 3 cut(s) 349, 415, 476
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 4 cut(s) 324, 576, 900, 964
BstDEI CTNAG 3 cut(s) 178, 279, 540
BstF5I GGATG 4 cut(s) 298, 367, 576, 811
BstKTI GATC 4 cut(s) 26, 217, 451, 621
BstMAI GTCTC 1 cut(s) 845
BstMBI GATC 4 cut(s) 23, 214, 448, 618
BstMWI GCNNNNNNNGC 3 cut(s) 271, 747, 783
BstNI CCWGG 1 cut(s) 375
BstNSI RCATGY 2 cut(s) 804, 902
BstPAI GACNNNNGTC 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 373, 435
BstSFI CTRYAG 2 cut(s) 237, 582
BstV1I GCAGC 3 cut(s) 565, 568, 941
BstV2I GAAGAC 1 cut(s) 799
BstX2I RGATCY 2 cut(s) 23, 448
BstYI RGATCY 2 cut(s) 23, 448
BsuI GTATCC 1 cut(s) 610
BtsCI GGATG 4 cut(s) 298, 367, 576, 811
BtsIMutI CAGTG 3 cut(s) 201, 287, 481
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 4 cut(s) 324, 576, 900, 964
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 2 cut(s) 793, 921
Csp6I GTAC 1 cut(s) 390
CviAII CATG 7 cut(s) 212, 801, 871, 899, 905, 949, 968
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 3 cut(s) 178, 279, 540
DpnI GATC 4 cut(s) 25, 216, 450, 620
DpnII GATC 4 cut(s) 23, 214, 448, 618
DrdI GACNNNNNNGTC 2 cut(s) 11, 347
DseDI GACNNNNNNGTC 2 cut(s) 11, 347
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 7 cut(s) 215, 804, 874, 902, 908, 952, 971
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 7 cut(s) 211, 800, 870, 898, 904, 948, 967
FblI GTMKAC 2 cut(s) 236, 260
Fnu4HI GCNGC 3 cut(s) 579, 582, 930
FokI GGATG 4 cut(s) 305, 374, 583, 798
Fsp4HI GCNGC 3 cut(s) 579, 582, 930
FspBI CTAG 6 cut(s) 75, 309, 323, 698, 914, 963
GluI GCNGC 3 cut(s) 579, 582, 930
GsaI CCCAGC 1 cut(s) 679
GsuI CTGGAG 2 cut(s) 511, 727
HapII CCGG 1 cut(s) 437
HgaI GACGC 2 cut(s) 793, 921
Hin1II CATG 7 cut(s) 215, 804, 874, 902, 908, 952, 971
HinfI GANTC 6 cut(s) 44, 71, 242, 300, 680, 716
HpaII CCGG 1 cut(s) 437
HphI GGTGA 2 cut(s) 599, 701
Hpy166II GTNNAC 2 cut(s) 237, 261
Hpy188I TCNGA 4 cut(s) 100, 127, 543, 798
Hpy188III TCNNGA 1 cut(s) 499
Hpy8I GTNNAC 2 cut(s) 237, 261
HpyAV CCTTC 2 cut(s) 256, 561
HpyCH4III ACNGT 3 cut(s) 349, 415, 476
HpyCH4V TGCA 6 cut(s) 146, 536, 584, 630, 898, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 747, 783
HpyF3I CTNAG 3 cut(s) 178, 279, 540
Hsp92II CATG 7 cut(s) 215, 804, 874, 902, 908, 952, 971
Kzo9I GATC 4 cut(s) 23, 214, 448, 618
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 746
Lsp1109I GCAGC 3 cut(s) 565, 568, 941
LweI GCATC 2 cut(s) 55, 561
MaeI CTAG 6 cut(s) 75, 309, 323, 698, 914, 963
MaeIII GTNAC 2 cut(s) 170, 420
MalI GATC 4 cut(s) 25, 216, 450, 620
MboI GATC 4 cut(s) 23, 214, 448, 618
MboII GAAGA 6 cut(s) 281, 324, 443, 675, 799, 912
MflI RGATCY 2 cut(s) 23, 448
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 5 cut(s) 37, 606, 638, 664, 791
MlyI GAGTC 3 cut(s) 65, 251, 710
MnlI CCTC 6 cut(s) 25, 104, 439, 540, 567, 712
MseI TTAA 2 cut(s) 636, 773
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 375, 437
Mva1269I GAATGC 3 cut(s) 274, 403, 764
MvaI CCWGG 1 cut(s) 375
MwoI GCNNNNNNNGC 3 cut(s) 271, 747, 783
NciI CCSGG 1 cut(s) 437
NdeII GATC 4 cut(s) 23, 214, 448, 618
NheI GCTAGC 2 cut(s) 322, 962
NlaIII CATG 7 cut(s) 215, 804, 874, 902, 908, 952, 971
NlaIV GGNNCC 1 cut(s) 742
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 170, 420
NspI RCATGY 2 cut(s) 804, 902
PaeI GCATGC 1 cut(s) 902
PaqCI CACCTGC 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 264
PctI GAATGC 3 cut(s) 274, 403, 764
PfeI GAWTC 3 cut(s) 44, 300, 680
PkrI GCNGC 3 cut(s) 580, 583, 931
PleI GAGTC 3 cut(s) 65, 250, 710
PpsI GAGTC 3 cut(s) 65, 250, 710
PshAI GACNNNNGTC 1 cut(s) 803
Psp6I CCWGG 1 cut(s) 373
PspFI CCCAGC 1 cut(s) 675
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 742
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 2 cut(s) 23, 448
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 903
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 636, 773
SatI GCNGC 3 cut(s) 579, 582, 930
Sau3AI GATC 4 cut(s) 23, 214, 448, 618
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 3 cut(s) 65, 251, 710
ScrFI CCNGG 2 cut(s) 375, 437
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 55, 561
SfcI CTRYAG 2 cut(s) 237, 582
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 903
SphI GCATGC 1 cut(s) 902
Sse9I AATT 5 cut(s) 37, 606, 638, 664, 791
SsiI CCGC 1 cut(s) 336
SspMI CTAG 6 cut(s) 75, 309, 323, 698, 914, 963
StyD4I CCNGG 2 cut(s) 373, 435
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 349, 415, 476
TaqI TCGA 4 cut(s) 15, 162, 217, 298
TaqII GACCGA 1 cut(s) 21
TasI AATT 5 cut(s) 37, 606, 638, 664, 791
TfiI GAWTC 3 cut(s) 44, 300, 680
Tru1I TTAA 2 cut(s) 636, 773
Tru9I TTAA 2 cut(s) 636, 773
TscAI CASTG 3 cut(s) 208, 287, 481
TseFI GTSAC 2 cut(s) 170, 420
TseI GCWGC 3 cut(s) 578, 581, 929
Tsp45I GTSAC 2 cut(s) 170, 420
TspGWI ACGGA 2 cut(s) 36, 305
TspRI CASTG 3 cut(s) 208, 287, 481
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 791
XceI RCATGY 2 cut(s) 804, 902
XmiI GTMKAC 2 cut(s) 236, 260
XspI CTAG 6 cut(s) 75, 309, 323, 698, 914, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.