Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002590

Physical Location & Seq

Genomic Coordinates Forward (+)

17762602 .. 17765204

Gene Structure 2603 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1219700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGCACCATCGGTGCACTGTAAGACATAGTGCCGTCGTTACAGTAGAGCTGAGGCGGCGGCGTGTGGGTTTTTCAAGGACAGAGGAGTCGGAGAGAAAGAAGAAGAAGAAGGCGCGTGGGAGGTGGAGTGGTCGAAGGTCAGGGATCGAGTGTGGCGGGGTGGCAGTGGAGACGAAGCTGGGGAATGAAACGCCCAAATTGATTTTTTTTTATTTTATTTTTTTTATAATTTTTTTGTTAGTTTTTTATTTTTTTTCGTTATCTTGTGATTTGTATTATGCAGGAACATTTCGATGGCTAGGAACTGTAAAAGTTATTCTTACTAACTTGGTGCCGCCCCAAACCCGTTTGCAGTTGGGCTCAGAGATAAAGGATGATTATGCAGATAAATCTGCACTATCAGAACAAGAAATAGATGTGGGCAAAGGGGTAGCAACATTTACCCAAAAAGATACTGTAAAAGAAGAATTACGACTCGGAGCTCCACCAGGTTCTGAACAAGGCTCTGACGAAAGTGAAGAGATTAGTGGTGATATAGCATCAAACCAGGTTGAGAAAAGTGGGGTTTTAACAAGTGGGTCAAAAGAAAGAGAAGAAAATAAAGAAGTTCAGGAAGCGGAAATAGATGGCAAGGCAGTTGATGCAAGACAGAAAGAAAAAGCAGTGGAAAATAATTCAGATGTACAAAAGCAAAACGTGGAGCTAGGGACATCATTAGCTCCAGTCTGCAATTATGCGAAAGGAAAATGGGTTGTGGACCATAAGAAGCCGCTGTATTTTGGGCATGGCTGTAAGCAATGGCTGTCAAGTATGTGGTATTACCGGATGACACAGCGCACAGATTTTTCCTACGAGAAGGTTTCATGGCAGCCAAAAGATTGTCAAATGGAAGAATTTGAAGGCTCTAAGTTCTTGAAAAGGATGCAACACAAAACTCTAGCTTTTGTTGGAATTTCAATGGGCCGACAGCAGTTCCAGTCTTTAATGTGCATGATCACAGGTGGTAAGGTGAGGCATGATGTTATAGATGTGGGGCCGGAATACGGGATAGTCCAAGCACGTGGCGATGTCCGCCCTAGTGGGTGGGCATATCGGTTCCCAAGCACCAACACTACCATCCTCTATTCCTGGTCATCAACCCTCTGTGATCTGCAGCCTATTGATGCTTCAAACCCAGCCACTCATTATGCCATGCACCTTGATCGGCCACCCGCATTCTTGTGCCAATATATTCATAAACTTAATGTCCTGGTCCTCAATACAGGGCACCACTGGAACCGTGGAAAGCTTAAAGCTAACCGTTGGGTGATGCATCTTGGGGGTGTCCGAAACACTGACAGGAAGATAGCTGTGATTTGGAAGGCCAAGAATGTGACAGTCCACAGTATCATTAATTGGGTGAACTCGCAGCTCCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0071554 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

473

Amino Acids

53.79

Weight (kDa)

9.27

Isoelectric Point (pI)

50.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	243 - 295	2.9e-11	PMR5 N terminal Domain
PC-Esterase	PF13839	298 - 470	9.6e-28	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002590)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20680 AT5G20680 AT5G20680 AT5G20680 AT5G20680 AT5G20680
fragaria_vesca	FvH4_1g08950 FvH4_1g08950 FvH4_1g08950 FvH4_1g08950 FvH4_1g08950 FvH4_1g08950 FvH4_1g08950
malus_domestica	MD02G1094800.v1.1 MD02G1095000.v1.1 MD15G1219700.v1.1
prunus_persica	Prupe.7G196200_v2.0.a1
pyrus_communis	pycom15g19490
rosa_chinensis	RchiOBHm_Chr2g0095401 RchiOBHm_Chr6g0266541 RchiOBHm_Chr7g0215131
rosa_laevigata	RLG00000016562 RLG00000028143 RLG00000028147
rosa_multiflora	Rmu_co8443815.1_g000001 Rmu_sc0002037.1_g000019 Rmu_sc0004484.1_g000007 Rmu_sc0040132.1_g000001 Rmu_ssc0000183.1_g000043
rosa_roxburghii	Rroxscaffold_2G00146400
rosa_rugosa	Rorug01G0247000 Rorug02G0050500
rosa_samantha	Rh1BG229300 Rh1DG257200 Rh2AG098700 Rh2BG099900 Rh2CG101900 Rh2DG099500 Rh7AG287300
rosa_wichuraiana	Rw2G007450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	227
AasI	GACNNNNNNGTC	1 cut(s)	85
AccB1I	GGYRCC	2 cut(s)	331, 1266
AccII	CGCG	1 cut(s)	115
AciI	CCGC	8 cut(s)	55, 58, 156, 335, 617, 770, 1072, 1212
AclWI	GGATC	1 cut(s)	152
AcoI	YGGCCR	1 cut(s)	1205
AcsI	RAATTY	2 cut(s)	893, 951
AcvI	CACGTG	1 cut(s)	1061
AfaI	GTAC	1 cut(s)	684
AfiI	CCNNNNNNNGG	1 cut(s)	1043
AgsI	TTSAA	5 cut(s)	75, 899, 916, 957, 1170
AjnI	CCWGG	4 cut(s)	487, 546, 1127, 1248
Alw21I	GWGCWC	2 cut(s)	17, 484
Alw26I	GTCTC	1 cut(s)	164
Alw44I	GTGCAC	1 cut(s)	13
AlwI	GGATC	1 cut(s)	152
AlwNI	CAGNNNCTG	1 cut(s)	494
AoxI	GGCC	4 cut(s)	961, 1034, 1205, 1362
ApaLI	GTGCAC	1 cut(s)	13
ApeKI	GCWGC	3 cut(s)	868, 1153, 1408
ApoI	RAATTY	2 cut(s)	893, 951
AseI	ATTAAT	1 cut(s)	1392
AspLEI	GCGC	2 cut(s)	115, 837
AspS9I	GGNCC	4 cut(s)	757, 961, 1034, 1252
AsuHPI	GGTGA	4 cut(s)	542, 1021, 1318, 1411
AvaII	GGWCC	2 cut(s)	757, 1252
BaeGI	GKGCMC	2 cut(s)	17, 1269
BanI	GGYRCC	2 cut(s)	331, 1266
BanII	GRGCYC	2 cut(s)	362, 484
BbrPI	CACGTG	1 cut(s)	1061
Bbv12I	GWGCWC	2 cut(s)	17, 484
BbvCI	CCTCAGC	1 cut(s)	50
BbvI	GCAGC	2 cut(s)	880, 1165
BccI	CCATC	4 cut(s)	15, 288, 620, 1124
BceAI	ACGGC	1 cut(s)	17
BcgI	CGANNNNNNTGC	2 cut(s)	1184, 1218
BciT130I	CCWGG	4 cut(s)	489, 548, 1129, 1250
BclI	TGATCA	1 cut(s)	993
BcoDI	GTCTC	1 cut(s)	164
BfaI	CTAG	4 cut(s)	299, 704, 938, 1077
BfmI	CTRYAG	1 cut(s)	1151
BisI	GCNGC	7 cut(s)	56, 59, 335, 770, 869, 1154, 1409
BlsI	GCNGC	7 cut(s)	57, 60, 336, 771, 870, 1155, 1410
Bme1390I	CCNGG	4 cut(s)	489, 548, 1129, 1250
Bme18I	GGWCC	2 cut(s)	757, 1252
BmgT120I	GGNCC	4 cut(s)	757, 961, 1034, 1252
BmiI	GGNNCC	5 cut(s)	333, 1035, 1097, 1268, 1277
BmrFI	CCNGG	4 cut(s)	489, 548, 1129, 1250
BmsI	GCATC	6 cut(s)	548, 631, 912, 1153, 1299, 1321
BpmI	CTGGAG	1 cut(s)	705
Bpu10I	CCTNAGC	1 cut(s)	50
BsaAI	YACGTR	1 cut(s)	1061
BsaJI	CCNNGG	1 cut(s)	1279
BsaWI	WCCGGW	1 cut(s)	822
BsaXI	ACNNNNNCTCC	2 cut(s)	112, 142
Bsc4I	CCNNNNNNNGG	1 cut(s)	1043
Bse1I	ACTGG	3 cut(s)	722, 976, 1277
Bse3DI	GCAATG	1 cut(s)	803
BseBI	CCWGG	4 cut(s)	489, 548, 1129, 1250
BseDI	CCNNGG	1 cut(s)	1279
BseGI	GGATG	4 cut(s)	379, 831, 927, 1116
BseLI	CCNNNNNNNGG	1 cut(s)	1043
BseMI	GCAATG	1 cut(s)	803
BseMII	CTCAG	2 cut(s)	41, 375
BseNI	ACTGG	3 cut(s)	722, 976, 1277
BseRI	GAGGAG	1 cut(s)	98
BseSI	GKGCMC	2 cut(s)	17, 1269
BseXI	GCAGC	2 cut(s)	880, 1165
BseYI	CCCAGC	2 cut(s)	178, 1174
BsgI	GTGCAG	1 cut(s)	378
Bsh1236I	CGCG	1 cut(s)	115
BshFI	GGCC	4 cut(s)	963, 1036, 1207, 1364
BshNI	GGYRCC	2 cut(s)	331, 1266
BsiHKAI	GWGCWC	2 cut(s)	17, 484
BsiSI	CCGG	2 cut(s)	823, 1037
BslFI	GGGAC	1 cut(s)	721
BslI	CCNNNNNNNGG	1 cut(s)	1043
BsmAI	GTCTC	1 cut(s)	164
BsmBI	CGTCTC	1 cut(s)	164
BsmFI	GGGAC	1 cut(s)	721
BsmI	GAATGC	1 cut(s)	1214
BsnI	GGCC	4 cut(s)	963, 1036, 1207, 1364
Bsp1286I	GDGCHC	4 cut(s)	17, 362, 484, 1269
Bsp1407I	TGTACA	1 cut(s)	682
Bsp143I	GATC	4 cut(s)	144, 993, 1147, 1201
BspACI	CCGC	8 cut(s)	55, 58, 156, 335, 617, 770, 1072, 1212
BspANI	GGCC	4 cut(s)	963, 1036, 1207, 1364
BspCNI	CTCAG	2 cut(s)	42, 374
BspFNI	CGCG	1 cut(s)	115
BspLI	GGNNCC	5 cut(s)	333, 1035, 1097, 1268, 1277
BspMAI	CTGCAG	1 cut(s)	1155
BspPI	GGATC	1 cut(s)	152
BspT107I	GGYRCC	2 cut(s)	331, 1266
BsrDI	GCAATG	1 cut(s)	803
BsrGI	TGTACA	1 cut(s)	682
BsrI	ACTGG	3 cut(s)	722, 976, 1277
BssECI	CCNNGG	1 cut(s)	1279
BssMI	GATC	4 cut(s)	144, 993, 1147, 1201
Bst2UI	CCWGG	4 cut(s)	489, 548, 1129, 1250
Bst4CI	ACNGT	8 cut(s)	19, 43, 307, 457, 1280, 1301, 1378, 1385
Bst6I	CTCTTC	1 cut(s)	513
BstAPI	GCANNNNNTGC	1 cut(s)	641
BstAUI	TGTACA	1 cut(s)	682
BstBAI	YACGTR	1 cut(s)	1061
BstDEI	CTNAG	3 cut(s)	50, 361, 906
BstDSI	CCRYGG	1 cut(s)	1279
BstF5I	GGATG	4 cut(s)	379, 831, 927, 1116
BstFNI	CGCG	1 cut(s)	115
BstHHI	GCGC	2 cut(s)	115, 837
BstKTI	GATC	4 cut(s)	147, 996, 1150, 1204
BstMAI	GTCTC	1 cut(s)	164
BstMBI	GATC	4 cut(s)	144, 993, 1147, 1201
BstMWI	GCNNNNNNNGC	3 cut(s)	55, 641, 1071
BstNI	CCWGG	4 cut(s)	489, 548, 1129, 1250
BstSCI	CCNGG	4 cut(s)	487, 546, 1127, 1248
BstSFI	CTRYAG	1 cut(s)	1151
BstSLI	GKGCMC	2 cut(s)	17, 1269
BstUI	CGCG	1 cut(s)	115
BstV1I	GCAGC	2 cut(s)	880, 1165
BstXI	CCANNNNNNTGG	1 cut(s)	1061
BsuRI	GGCC	4 cut(s)	963, 1036, 1207, 1364
BtgI	CCRYGG	1 cut(s)	1279
BtgZI	GCGATG	1 cut(s)	1080
BtsCI	GGATG	4 cut(s)	379, 831, 927, 1116
BtsI	GCAGTG	2 cut(s)	171, 669
BtsIMutI	CAGTG	5 cut(s)	15, 171, 669, 1270, 1332
CaiI	CAGNNNCTG	1 cut(s)	494
CfoI	GCGC	2 cut(s)	115, 837
Cfr13I	GGNCC	4 cut(s)	757, 961, 1034, 1252
CsiI	ACCWGGT	2 cut(s)	487, 546
Csp6I	GTAC	1 cut(s)	683
CviAII	CATG	5 cut(s)	785, 864, 991, 1016, 1192
CviQI	GTAC	1 cut(s)	683
DdeI	CTNAG	3 cut(s)	50, 361, 906
DpnI	GATC	4 cut(s)	146, 995, 1149, 1203
DpnII	GATC	4 cut(s)	144, 993, 1147, 1201
DrdI	GACNNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	85
EaeI	YGGCCR	1 cut(s)	1205
Eam1104I	CTCTTC	1 cut(s)	513
EarI	CTCTTC	1 cut(s)	513
EciI	GGCGGA	1 cut(s)	1061
Ecl136II	GAGCTC	1 cut(s)	482
Eco24I	GRGCYC	2 cut(s)	362, 484
Eco47I	GGWCC	2 cut(s)	757, 1252
Eco53kI	GAGCTC	1 cut(s)	482
Eco72I	CACGTG	1 cut(s)	1061
EcoICRI	GAGCTC	1 cut(s)	482
EcoRII	CCWGG	4 cut(s)	487, 546, 1127, 1248
EcoT22I	ATGCAT	1 cut(s)	1314
EcoT38I	GRGCYC	2 cut(s)	362, 484
Esp3I	CGTCTC	1 cut(s)	164
FaeI	CATG	5 cut(s)	788, 867, 994, 1019, 1195
FalI	AAGNNNNNCTT	2 cut(s)	303, 335
FaqI	GGGAC	1 cut(s)	721
FatI	CATG	5 cut(s)	784, 863, 990, 1015, 1191
FauI	CCCGC	2 cut(s)	149, 1219
FbaI	TGATCA	1 cut(s)	993
Fnu4HI	GCNGC	7 cut(s)	56, 59, 335, 770, 869, 1154, 1409
FokI	GGATG	4 cut(s)	386, 838, 934, 1103
FriOI	GRGCYC	2 cut(s)	362, 484
Fsp4HI	GCNGC	7 cut(s)	56, 59, 335, 770, 869, 1154, 1409
FspBI	CTAG	4 cut(s)	299, 704, 938, 1077
GlaI	GCGC	2 cut(s)	114, 836
GluI	GCNGC	7 cut(s)	56, 59, 335, 770, 869, 1154, 1409
GsaI	CCCAGC	2 cut(s)	182, 1178
GsuI	CTGGAG	1 cut(s)	705
HaeIII	GGCC	4 cut(s)	963, 1036, 1207, 1364
HapII	CCGG	2 cut(s)	823, 1037
HhaI	GCGC	2 cut(s)	115, 837
Hin1II	CATG	5 cut(s)	788, 867, 994, 1019, 1195
Hin6I	GCGC	2 cut(s)	113, 835
HinP1I	GCGC	2 cut(s)	113, 835
HindIII	AAGCTT	1 cut(s)	1286
HinfI	GANTC	2 cut(s)	86, 474
HpaII	CCGG	2 cut(s)	823, 1037
HphI	GGTGA	4 cut(s)	542, 1021, 1318, 1411
Hpy166II	GTNNAC	4 cut(s)	15, 757, 1381, 1402
Hpy188I	TCNGA	8 cut(s)	91, 364, 403, 479, 496, 508, 679, 1328
Hpy188III	TCNNGA	2 cut(s)	611, 913
Hpy8I	GTNNAC	4 cut(s)	15, 757, 1381, 1402
Hpy99I	CGWCG	1 cut(s)	38
HpyAV	CCTTC	5 cut(s)	103, 129, 850, 893, 1354
HpyCH4III	ACNGT	8 cut(s)	19, 43, 307, 457, 1280, 1301, 1378, 1385
HpyCH4IV	ACGT	2 cut(s)	696, 1060
HpyF10VI	GCNNNNNNNGC	3 cut(s)	55, 641, 1071
HpyF3I	CTNAG	3 cut(s)	50, 361, 906
HpySE526I	ACGT	2 cut(s)	696, 1060
Hsp92II	CATG	5 cut(s)	788, 867, 994, 1019, 1195
HspAI	GCGC	2 cut(s)	113, 835
Ksp22I	TGATCA	1 cut(s)	993
Kzo9I	GATC	4 cut(s)	144, 993, 1147, 1201
LmnI	GCTCC	5 cut(s)	479, 487, 700, 724, 1416
Lsp1109I	GCAGC	2 cut(s)	880, 1165
LweI	GCATC	6 cut(s)	548, 631, 912, 1153, 1299, 1321
MabI	ACCWGGT	2 cut(s)	487, 546
MaeI	CTAG	4 cut(s)	299, 704, 938, 1077
MaeII	ACGT	2 cut(s)	696, 1060
MaeIII	GTNAC	2 cut(s)	37, 1372
MalI	GATC	4 cut(s)	146, 995, 1149, 1203
MboI	GATC	4 cut(s)	144, 993, 1147, 1201
MboII	GAAGA	8 cut(s)	112, 115, 118, 476, 530, 605, 902, 1354
MhlI	GDGCHC	4 cut(s)	17, 362, 484, 1269
MluCI	AATT	8 cut(s)	197, 228, 467, 673, 730, 893, 951, 1393
MlyI	GAGTC	2 cut(s)	95, 468
MmeI	TCCRAC	2 cut(s)	69, 928
MnlI	CCTC	7 cut(s)	45, 76, 114, 1005, 1130, 1151, 1265
Mph1103I	ATGCAT	1 cut(s)	1314
MseI	TTAA	5 cut(s)	569, 983, 1242, 1290, 1392
MslI	CAYNNNNRTG	2 cut(s)	292, 1219
MspA1I	CMGCKG	1 cut(s)	772
MspI	CCGG	2 cut(s)	823, 1037
MspR9I	CCNGG	4 cut(s)	489, 548, 1129, 1250
Mva1269I	GAATGC	1 cut(s)	1214
MvaI	CCWGG	4 cut(s)	489, 548, 1129, 1250
MvnI	CGCG	1 cut(s)	115
MwoI	GCNNNNNNNGC	3 cut(s)	55, 641, 1071
NdeII	GATC	4 cut(s)	144, 993, 1147, 1201
NlaIII	CATG	5 cut(s)	788, 867, 994, 1019, 1195
NlaIV	GGNNCC	5 cut(s)	333, 1035, 1097, 1268, 1277
NmuCI	GTSAC	1 cut(s)	1372
NsiI	ATGCAT	1 cut(s)	1314
PctI	GAATGC	1 cut(s)	1214
PkrI	GCNGC	7 cut(s)	57, 60, 336, 771, 870, 1155, 1410
PleI	GAGTC	2 cut(s)	94, 468
PmaCI	CACGTG	1 cut(s)	1061
PmlI	CACGTG	1 cut(s)	1061
PpsI	GAGTC	2 cut(s)	94, 468
Ppu21I	YACGTR	1 cut(s)	1061
PshBI	ATTAAT	1 cut(s)	1392
PsiI	TTATAA	1 cut(s)	227
Psp124BI	GAGCTC	1 cut(s)	484
Psp6I	CCWGG	4 cut(s)	487, 546, 1127, 1248
PspCI	CACGTG	1 cut(s)	1061
PspFI	CCCAGC	2 cut(s)	178, 1174
PspGI	CCWGG	4 cut(s)	487, 546, 1127, 1248
PspN4I	GGNNCC	5 cut(s)	333, 1035, 1097, 1268, 1277
PspPI	GGNCC	4 cut(s)	757, 961, 1034, 1252
PstI	CTGCAG	1 cut(s)	1155
PstNI	CAGNNNCTG	1 cut(s)	494
RsaI	GTAC	1 cut(s)	684
RsaNI	GTAC	1 cut(s)	683
RseI	CAYNNNNRTG	2 cut(s)	292, 1219
SacI	GAGCTC	1 cut(s)	484
SaqAI	TTAA	5 cut(s)	569, 983, 1242, 1290, 1392
SatI	GCNGC	7 cut(s)	56, 59, 335, 770, 869, 1154, 1409
Sau3AI	GATC	4 cut(s)	144, 993, 1147, 1201
Sau96I	GGNCC	4 cut(s)	757, 961, 1034, 1252
SchI	GAGTC	2 cut(s)	95, 468
ScrFI	CCNGG	4 cut(s)	489, 548, 1129, 1250
SduI	GDGCHC	4 cut(s)	17, 362, 484, 1269
SexAI	ACCWGGT	2 cut(s)	487, 546
SfaNI	GCATC	6 cut(s)	548, 631, 912, 1153, 1299, 1321
SfcI	CTRYAG	1 cut(s)	1151
SinI	GGWCC	2 cut(s)	757, 1252
SmiMI	CAYNNNNRTG	2 cut(s)	292, 1219
Sse9I	AATT	8 cut(s)	197, 228, 467, 673, 730, 893, 951, 1393
SsiI	CCGC	8 cut(s)	55, 58, 156, 335, 617, 770, 1072, 1212
SspMI	CTAG	4 cut(s)	299, 704, 938, 1077
SstI	GAGCTC	1 cut(s)	484
StyD4I	CCNGG	4 cut(s)	487, 546, 1127, 1248
TaaI	ACNGT	8 cut(s)	19, 43, 307, 457, 1280, 1301, 1378, 1385
TaiI	ACGT	2 cut(s)	699, 1063
TaqI	TCGA	3 cut(s)	133, 147, 292
TasI	AATT	8 cut(s)	197, 228, 467, 673, 730, 893, 951, 1393
TatI	WGTACW	1 cut(s)	682
TauI	GCSGC	4 cut(s)	58, 61, 337, 772
Tru1I	TTAA	5 cut(s)	569, 983, 1242, 1290, 1392
Tru9I	TTAA	5 cut(s)	569, 983, 1242, 1290, 1392
TscAI	CASTG	5 cut(s)	22, 171, 669, 1277, 1339
TseFI	GTSAC	1 cut(s)	1372
TseI	GCWGC	3 cut(s)	868, 1153, 1408
Tsp45I	GTSAC	1 cut(s)	1372
TspDTI	ATGAA	3 cut(s)	201, 852, 1223
TspRI	CASTG	5 cut(s)	22, 171, 669, 1277, 1339
VneI	GTGCAC	1 cut(s)	13
VpaK11BI	GGWCC	2 cut(s)	757, 1252
VspI	ATTAAT	1 cut(s)	1392
XapI	RAATTY	2 cut(s)	893, 951
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1277
XspI	CTAG	4 cut(s)	299, 704, 938, 1077
Zsp2I	ATGCAT	1 cut(s)	1314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.