MD15G1228100.v1.1

trafficking protein particle complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011992
Physical Location & Seq
Forward (+)
18581264 .. 18586745
5482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1228100.v1.1.491

Sequence Viewer

Length: 3600 bp
ATGGAAGACTACCCAGAAGAAATGAGGAGTCCGCCGGTGAGCCTGGTGTCAGTGGTGGGATGCCCGGAGCTCCACACCTCAATCTCCGCCCACCTCCACTCTCTCTCCCCACCAATCAACACCCTCGCCTTTCCGGACCTCTCCAAGGCCTCACTCATCCTACCCCCAAAGCCCAACCCTACTTCCACTCCAGCCTCCGATTCCTCCGCCCCTCCACCCGCCGGAATAATAAAGCGAGATTGGCTCCTCAAGCACCGCACCAAAATCCCCGCCGTCGTCGCCGCCCTCCTCTCATCCGATCGGGTCACTGGCGACCCGGCTCAGTGGCTCCAACTCTGTTCCGACCTCGACGGCCTCAAAGCCCTCCTCCGCGGCAGAAACATCAAGCTGGTCGTCGTCGTCGTCTACTCCAATCCCAGAGATGAAATCTCGGAGGATCAGATGGTGGCTGTGCGGAAGCGCGCGGAGGTGGATGCCAAGTATCTTCTCACGTTTTACCGCAACCCAGATGGCAGCAGCGATGGTTCTCAGTTCAAAGAGTCTCTCCATAGACTGGGAAACGTGTTTGCAGAGCTGGCCGGATTGTATTACAGAGACGAAGGACGAAGGGTTAGAGCTCGCATTGAGAGGAAGTCTTCCAATCCGGCTGACCTCAACATTCGCTATTCCTTCAAAGTTGCTGTGTATGCTGAGTTCAGAAGAGATTGGGTTGAAGCTCTGAGGTTTTATGAGGATGCTTATCACACCCTTCGGGAGTTAATTGCTGGGGCATCAACTAGTGTGTCGGTGATTCAACGGTTAGTTGAGATAAAAACTATTGCGGAGCAATTGCATTTTAAAATTTCAACCTTGTTATTGCATGGTGGGAAGATTGTAGAAGCAGTTGTGTGGTTCCGCCTGCACAATGCTTCATACAGAAAGCTCATAGGAGCACCAGAAGCTATATTTCTTCACTGGGAGTGGATGGGTAGACAGTTCTTGGTGTTTGCTGAATTGTTGGAGACGAGTTCAACAGCCATTCAAAGCATTTCACCTCTTCCTGTAGGCACAGCAGACAGACCTTTGACTGAGTGGGAACTTCAGCCAGCTCATTACTATCAGTTAGCTGCTCATTACTTGAAGGAGAAGAGATCTTCTTTGGAATTTGCAGTTTCAATGTCAGAAGGTGATATCGATTGCAGTGCTGAATCTGTGGTACCTTCATCTTATTTGGGTCAGTTTGCTCGCTTAATTGAGCAAGGAGATACATTTGTCATGCAACCCCTTACTGATGAAGAGTACATGCGTTATGCTATTTCTGAAGGAAAAAGGTTCCAAGATTCCTTTGAAATTATTGCGTTACTAAAAAAATCTTGTGAATCGTACAATAATCGCAAAGTCAGGAGAATGGGCTCATTTTGTGGATTCCAGATGGCTAGAGAATATTATGCTCTAGGTGATTTTAGCAATGCGAAACAGCTTTTTGATGACATTGCAAGTCTATACAGACAAGAGGGTTGGGTCATTTTGTTGTGGGAGGTATTGGGTTATCTGCGTGAATGTTCAAAGAGACAGAGTAAAGTTAAAGATTTTATGGAGTACTCCTTTGAAATGGCTGCGCTCCCTATATCAGCTGATACTGGTATCCAGTCTTTCCGATTTGAAGAATCTGGTCCAGCTGGGCCTGCGACTCTCCAACAAAGAGAAACAATACACAAAGAAGTTTTTGGGCTTGTTAGTGGAGAACTGAGATTGGCATCAACTGAAAATGGTAATGATCTGAAAGTATCTGGTGAAAGTCCACTTCACCTTGAAGTTGATCTTGTTAGTCCCCTCAGATTGGTACTTCTAGCTTCAGTTGCTTTTCATGAACAAATAATTAAGCCTGGCTCATCCACATTGGTTACGCTGTCATTTCTGTCACAGTTACCCCTTAATTTCGAGATTGATCAGTTAGAAGTCCAGTTCAATCAGTCTGACTGTAACTTCATCATCATGAATGGCCAAAGGCCTCGTGTAGCTGACATGAGTGATGGCCAACCGGGTTGCCGAGTAGAGACTGCTCCTTCTTTGGCACTTTCTACAAATAAGTGGCTGCGATTGACGTATAACATCAAATCTGATCAAAGCGGAAAGCTTGAATGCATATCTGTTGTTGCAAAAATAGGACCCCACTTCACAATCTGTTGCAGAGCTGAAAGTCCTGCTTCAATGGATGAATTGCCTCTTTGGAAATTTGAAGATCGCGTGGTCACCTATCCAACCAAGGACCCTGCTCTGGCATTCTCTGGTCAGAAGGCTACTCAGGTTGAAGAGTTAGACCCAGAAGTGGATCTTAGTTTAGGGTCTGCCGGCCCTGCACTTACCGGAGAGAGCTTCATAGTACCTGTTACTGTTACATCCAAGGGCCATGATGTTAATTCTGGTGAGTTGAAAATCAATCTGGTGGATGTAAGGGGAGGTGGTTTGTTTAGTCCAAGGGACACGGATCTATCTACGGATAGTCATCATGTTGAACTTCTTGGTATTTCTGGACCAGACGGAGGAGATGAGTCCCAACTGAACACTGATGAAATCAAGAAAATTCAACAGTCTTTTGGATTGGTTTCTGTTCCTGCTCTCAAAAGTGGAGACTCGTGGTCTTGCAAATTGGAAATCAAGTGGCACAGACCGAAACCAATTATGCTCTATGTATCATTGGGTTATTCTCCAGATAACAATGAAAACACTCAAAAGGTAAATGTCCACAAGAGCTTGCAGATTGAAGGAAAGAATGCAATCATTATTAGCCATCGCTTTATGCTTCCCTTCCGGAGGTATCCATTGTTACTTTCTAGGATAAAACCAGGTCCTGATTCTGACCTGTCAGCATCAATGCCTTTAAACGAAACAAGTGTACTAGTTGTTAGTGCTAAGAACTGCTCCGATGTGCCGCTTCAGTTGCTATCCTTATCTCTTGAAGCAGATGATAATGATGGCACCGAAAGGTCATGTTCTGTGAAACATGGAGGTCAGGACCTTTTAGACCCTGCCCTTCTAGTTCCAGGAGAAGAGTTCAAGAAGGTTTACACTGTCACTTCTGAGATGAATTCTTCAAAGCTCAGGTTAGGTAATGTGTGTCTTAGATGGAGAAGGGATTCTGGGACTGCAGTGGAATCTGGTTCTACGGCTTCAGTTTTAACTACACACAGACTTCCAGTTGTTAATCTAGAGTTGTCGCCTCTTGTTGTGAGTTTGGAATGTCCTCCTTATGCGATCCTTGGAGATCCTTTCACGTATTTTGTTAAAATACAGAACCAGACTGAATTGCTTCAGGAGGCCAAAATTTCATTAGCAGATGCACAAAGTTTTGTGTTAGCTGGGTCTCACAATGACAGCATTTTTATTCTTCCAAAATCTGAGCACATCGTCCGATACAAGCTTGTTCCACTTGCCTCAGGCGCACAACAGCTACCGAGATTCACGTTAACATCAGTTAGATATTCAACGGGGTTTCAGCCCTCAATTGCTGCATCGACAATTTTTGTATTTCCTTCTAAGCCTCATTTCAAGATGGCTGCTGTGGGAGATGGCAGGATGGCTGTGGGAGATGACAGGATGGAATCAGTGGTGGCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Foie-gras_1 Gryzun-like

Protein Analysis

1200

Amino Acids

133.0

Weight (kDa)

5.79

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Foie-gras_1 PF11817 266 - 533 1.9e-67 Foie gras liver health family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011992)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65950
fragaria_vesca FvH4_1g10000
malus_domestica MD15G1228100.v1.1
prunus_persica Prupe.7G186800_v2.0.a1 Prupe.7G186800_v2.0.a1
pyrus_communis pycom15g20290
rosa_chinensis RchiOBHm_Chr2g0096791
rosa_laevigata RLG00000016670 RLG00000023097
rosa_multiflora Rmu_co8505723.1_g000001 Rmu_sc0004938.1_g000018 Rmu_ssc0000215.1_g000031
rosa_roxburghii Rroxscaffold_2G00145310
rosa_rugosa Rorug02G0060900
rosa_samantha Rh2AG108200 Rh2BG110400 Rh2CG112300 Rh2DG111700
rosa_wichuraiana Rw2G008370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1195
AccB1I GGYRCC 2 cut(s) 1195, 2957
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 2 cut(s) 405, 970
AccII CGCG 4 cut(s) 372, 462, 464, 2226
AccIII TCCGGA 2 cut(s) 133, 2790
AclWI GGATC 5 cut(s) 444, 2319, 2475, 3229, 3239
AcoI YGGCCR 3 cut(s) 576, 1981, 2014
AcsI RAATTY 6 cut(s) 840, 1142, 2213, 2562, 3067, 3303
AcuI CTGAAG 6 cut(s) 1064, 1320, 1818, 2900, 3135, 3275
AfaI GTAC 7 cut(s) 1197, 1280, 1364, 1580, 1824, 2364, 2877
AfiI CCNNNNNNNGG 8 cut(s) 145, 221, 553, 1819, 2257, 2308, 2522, 2793
AflIII ACRYGT 1 cut(s) 561
AhdI GACNNNNNGTC 1 cut(s) 2617
AhlI ACTAGT 2 cut(s) 776, 2878
AjnI CCWGG 4 cut(s) 42, 1864, 2824, 3022
Alw21I GWGCWC 4 cut(s) 72, 619, 934, 3384
Alw26I GTCTC 7 cut(s) 546, 588, 995, 1543, 2030, 2604, 3348
AlwI GGATC 5 cut(s) 444, 2319, 2475, 3229, 3239
AlwNI CAGNNNCTG 1 cut(s) 2831
Aor13HI TCCGGA 2 cut(s) 133, 2790
ApeKI GCWGC 7 cut(s) 513, 516, 1106, 1595, 2074, 3488, 3536
ApoI RAATTY 6 cut(s) 840, 1142, 2213, 2562, 3067, 3303
Asp700I GAANNNNTTC 2 cut(s) 3115, 3288
Asp718I GGTACC 1 cut(s) 1195
AspLEI GCGC 4 cut(s) 462, 464, 1600, 3422
AsuC2I CCSGG 3 cut(s) 65, 317, 2022
AsuHPI GGTGA 9 cut(s) 49, 799, 1023, 1178, 1448, 1778, 1784, 2224, 2417
AvaII GGWCC 7 cut(s) 136, 1652, 2147, 2248, 2513, 2828, 2995
AxyI CCTNAGG 1 cut(s) 3415
BaeI ACNNNNGTAYC 2 cut(s) 1236, 1269
BalI TGGCCA 2 cut(s) 1983, 2016
BanI GGYRCC 2 cut(s) 1195, 2957
BanII GRGCYC 3 cut(s) 72, 619, 1394
BauI CACGAG 2 cut(s) 1992, 2614
BbsI GAAGAC 2 cut(s) 12, 627
Bbv12I GWGCWC 4 cut(s) 72, 619, 934, 3384
BbvI GCAGC 7 cut(s) 525, 528, 1093, 1582, 2061, 3475, 3523
BceAI ACGGC 3 cut(s) 257, 367, 3162
BcgI CGANNNNNNTGC 2 cut(s) 1163, 1197
BciT130I CCWGG 4 cut(s) 44, 1866, 2826, 3024
BciVI GTATCC 2 cut(s) 1634, 2808
BclI TGATCA 2 cut(s) 1927, 2101
BcnI CCSGG 3 cut(s) 65, 317, 2022
BcoDI GTCTC 7 cut(s) 546, 588, 995, 1543, 2030, 2604, 3348
BcuI ACTAGT 2 cut(s) 776, 2878
BfaI CTAG 8 cut(s) 777, 1416, 1433, 1829, 2814, 2879, 3017, 3188
BfmI CTRYAG 2 cut(s) 1041, 3126
BfuI GTATCC 2 cut(s) 1634, 2808
BglII AGATCT 1 cut(s) 1130
BmcAI AGTACT 1 cut(s) 1580
Bme1390I CCNGG 7 cut(s) 44, 65, 317, 1866, 2022, 2826, 3024
Bme18I GGWCC 7 cut(s) 136, 1652, 2147, 2248, 2513, 2828, 2995
BmeRI GACNNNNNGTC 1 cut(s) 2617
BmiI GGNNCC 8 cut(s) 245, 329, 893, 1197, 1313, 2149, 2250, 2959
BmrFI CCNGG 7 cut(s) 44, 65, 317, 1866, 2022, 2826, 3024
BmrI ACTGGG 2 cut(s) 563, 964
BmsI GCATC 8 cut(s) 50, 463, 724, 779, 1745, 2858, 3307, 3500
BmuI ACTGGG 2 cut(s) 563, 964
BpiI GAAGAC 2 cut(s) 12, 627
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 2 cut(s) 174, 2673
Bpu10I CCTNAGC 1 cut(s) 3080
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 3 cut(s) 65, 317, 2022
Bsa29I ATCGAT 1 cut(s) 1173
BsaAI YACGTR 1 cut(s) 3255
BsaBI GATNNNNATC 3 cut(s) 738, 1735, 2484
BsaI GGTCTC 1 cut(s) 3348
BsaJI CCNNGG 6 cut(s) 144, 370, 2244, 2382, 2456, 3238
BsaWI WCCGGW 3 cut(s) 133, 2345, 2790
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 172, 202
Bsc4I CCNNNNNNNGG 8 cut(s) 145, 221, 553, 1819, 2257, 2308, 2522, 2793
Bse118I RCCGGY 2 cut(s) 34, 2330
Bse1I ACTGG 7 cut(s) 313, 558, 959, 1624, 1627, 1942, 3176
Bse21I CCTNAGG 1 cut(s) 3415
Bse3DI GCAATG 2 cut(s) 1453, 1470
Bse8I GATNNNNATC 3 cut(s) 738, 1735, 2484
BseAI TCCGGA 2 cut(s) 133, 2790
BseBI CCWGG 4 cut(s) 44, 1866, 2826, 3024
BseCI ATCGAT 1 cut(s) 1173
BseDI CCNNGG 6 cut(s) 144, 370, 2244, 2382, 2456, 3238
BseJI GATNNNNATC 3 cut(s) 738, 1735, 2484
BseLI CCNNNNNNNGG 8 cut(s) 145, 221, 553, 1819, 2257, 2308, 2522, 2793
BseMI GCAATG 2 cut(s) 1453, 1470
BseNI ACTGG 7 cut(s) 313, 558, 959, 1624, 1627, 1942, 3176
BsePI GCGCGC 1 cut(s) 460
BseRI GAGGAG 5 cut(s) 40, 236, 278, 356, 2538
BseXI GCAGC 7 cut(s) 525, 528, 1093, 1582, 2061, 3475, 3523
BseYI CCCAGC 3 cut(s) 764, 1658, 3338
BsgI GTGCAG 2 cut(s) 884, 2322
Bsh1236I CGCG 4 cut(s) 372, 462, 464, 2226
Bsh1285I CGRYCG 1 cut(s) 301
BshNI GGYRCC 2 cut(s) 1195, 2957
BshVI ATCGAT 1 cut(s) 1173
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 4 cut(s) 72, 619, 934, 3384
BslFI GGGAC 4 cut(s) 1794, 2474, 2518, 3136
BslI CCNNNNNNNGG 8 cut(s) 145, 221, 553, 1819, 2257, 2308, 2522, 2793
BsmAI GTCTC 7 cut(s) 546, 588, 995, 1543, 2030, 2604, 3348
BsmBI CGTCTC 2 cut(s) 588, 995
BsmFI GGGAC 4 cut(s) 1794, 2474, 2518, 3136
BsmI GAATGC 3 cut(s) 2126, 2261, 2758
Bso31I GGTCTC 1 cut(s) 3348
Bsp1286I GDGCHC 5 cut(s) 72, 619, 934, 1394, 3384
Bsp13I TCCGGA 2 cut(s) 133, 2790
BspDI ATCGAT 1 cut(s) 1173
BspEI TCCGGA 2 cut(s) 133, 2790
BspFNI CGCG 4 cut(s) 372, 462, 464, 2226
BspHI TCATGA 2 cut(s) 1846, 1974
BspLI GGNNCC 8 cut(s) 245, 329, 893, 1197, 1313, 2149, 2250, 2959
BspMAI CTGCAG 1 cut(s) 3130
BspPI GGATC 5 cut(s) 444, 2319, 2475, 3229, 3239
BspT107I GGYRCC 2 cut(s) 1195, 2957
BspTNI GGTCTC 1 cut(s) 3348
BsrDI GCAATG 2 cut(s) 1453, 1470
BsrFI RCCGGY 2 cut(s) 34, 2330
BsrI ACTGG 7 cut(s) 313, 558, 959, 1624, 1627, 1942, 3176
BssAI RCCGGY 2 cut(s) 34, 2330
BssECI CCNNGG 6 cut(s) 144, 370, 2244, 2382, 2456, 3238
BssHII GCGCGC 1 cut(s) 460
BssSI CACGAG 2 cut(s) 1992, 2614
BssT1I CCWWGG 5 cut(s) 144, 2244, 2382, 2456, 3238
Bst2BI CACGAG 2 cut(s) 1992, 2614
Bst2UI CCWGG 4 cut(s) 44, 1866, 2826, 3024
Bst4CI ACNGT 7 cut(s) 798, 975, 1905, 1961, 2374, 2571, 3052
Bst6I CTCTTC 6 cut(s) 694, 1041, 1121, 1269, 2286, 3024
BstBAI YACGTR 1 cut(s) 3255
BstC8I GCNNGC 9 cut(s) 462, 576, 619, 899, 1086, 1225, 1665, 2332, 2735
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 2230
BstENI CCTNNNNNAGG 2 cut(s) 143, 2791
BstFNI CGCG 4 cut(s) 372, 462, 464, 2226
BstHHI GCGC 4 cut(s) 462, 464, 1600, 3422
BstMAI GTCTC 7 cut(s) 546, 588, 995, 1543, 2030, 2604, 3348
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 4 cut(s) 44, 1866, 2826, 3024
BstNSI RCATGY 1 cut(s) 1285
BstPI GGTNACC 1 cut(s) 2230
BstSCI CCNGG 7 cut(s) 42, 63, 315, 1864, 2020, 2824, 3022
BstSFI CTRYAG 2 cut(s) 1041, 3126
BstUI CGCG 4 cut(s) 372, 462, 464, 2226
BstV1I GCAGC 7 cut(s) 525, 528, 1093, 1582, 2061, 3475, 3523
BstV2I GAAGAC 2 cut(s) 12, 627
BstX2I RGATCY 4 cut(s) 1130, 2311, 2467, 3244
BstYI RGATCY 4 cut(s) 1130, 2311, 2467, 3244
Bsu15I ATCGAT 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 3415
BsuI GTATCC 2 cut(s) 1634, 2808
BsuTUI ATCGAT 1 cut(s) 1173
BtgI CCRYGG 1 cut(s) 370
BtgZI GCGATG 2 cut(s) 534, 2756
BtsI GCAGTG 2 cut(s) 1186, 3135
BtsIMutI CAGTG 9 cut(s) 57, 306, 329, 952, 1186, 2544, 3048, 3135, 3591
Cac8I GCNNGC 9 cut(s) 462, 576, 619, 899, 1086, 1225, 1665, 2332, 2735
CaiI CAGNNNCTG 1 cut(s) 2831
CciI TCATGA 2 cut(s) 1846, 1974
CfoI GCGC 4 cut(s) 462, 464, 1600, 3422
Cfr10I RCCGGY 2 cut(s) 34, 2330
Cfr42I CCGCGG 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 1173
CsiI ACCWGGT 1 cut(s) 2824
Csp6I GTAC 7 cut(s) 1196, 1279, 1363, 1579, 1823, 2363, 2876
CviQI GTAC 7 cut(s) 1196, 1279, 1363, 1579, 1823, 2363, 2876
DraI TTTAAA 2 cut(s) 838, 2862
DriI GACNNNNNGTC 1 cut(s) 2617
EaeI YGGCCR 3 cut(s) 576, 1981, 2014
Eam1104I CTCTTC 6 cut(s) 694, 1041, 1121, 1269, 2286, 3024
Eam1105I GACNNNNNGTC 1 cut(s) 2617
EarI CTCTTC 6 cut(s) 694, 1041, 1121, 1269, 2286, 3024
EciI GGCGGA 4 cut(s) 21, 76, 196, 884
Ecl136II GAGCTC 2 cut(s) 70, 617
Eco130I CCWWGG 5 cut(s) 144, 2244, 2382, 2456, 3238
Eco147I AGGCCT 2 cut(s) 149, 1990
Eco24I GRGCYC 3 cut(s) 72, 619, 1394
Eco31I GGTCTC 1 cut(s) 3348
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 7 cut(s) 136, 1652, 2147, 2248, 2513, 2828, 2995
Eco53kI GAGCTC 2 cut(s) 70, 617
Eco57I CTGAAG 6 cut(s) 1064, 1320, 1818, 2900, 3135, 3275
Eco81I CCTNAGG 1 cut(s) 3415
Eco91I GGTNACC 1 cut(s) 2230
EcoICRI GAGCTC 2 cut(s) 70, 617
EcoNI CCTNNNNNAGG 2 cut(s) 143, 2791
EcoO109I RGGNCCY 4 cut(s) 2147, 2248, 2828, 2995
EcoO65I GGTNACC 1 cut(s) 2230
EcoRI GAATTC 1 cut(s) 3067
EcoRII CCWGG 4 cut(s) 42, 1864, 2824, 3022
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 5 cut(s) 144, 2244, 2382, 2456, 3238
EcoT22I ATGCAT 1 cut(s) 2126
EcoT38I GRGCYC 3 cut(s) 72, 619, 1394
ErhI CCWWGG 5 cut(s) 144, 2244, 2382, 2456, 3238
Esp3I CGTCTC 2 cut(s) 588, 995
FalI AAGNNNNNCTT 6 cut(s) 1785, 1817, 2170, 2202, 2298, 2330
FaqI GGGAC 4 cut(s) 1794, 2474, 2518, 3136
FauI CCCGC 2 cut(s) 226, 277
FbaI TGATCA 2 cut(s) 1927, 2101
FblI GTMKAC 2 cut(s) 405, 970
FriOI GRGCYC 3 cut(s) 72, 619, 1394
FspBI CTAG 8 cut(s) 777, 1416, 1433, 1829, 2814, 2879, 3017, 3188
GlaI GCGC 4 cut(s) 461, 463, 1599, 3421
GsaI CCCAGC 3 cut(s) 768, 1662, 3342
GsuI CTGGAG 2 cut(s) 174, 2673
HhaI GCGC 4 cut(s) 462, 464, 1600, 3422
Hin6I GCGC 4 cut(s) 460, 462, 1598, 3420
HinP1I GCGC 4 cut(s) 460, 462, 1598, 3420
HincII GTYRAC 1 cut(s) 3447
HindII GTYRAC 1 cut(s) 3447
HindIII AAGCTT 2 cut(s) 2114, 3398
HpaI GTTAAC 1 cut(s) 3447
HphI GGTGA 9 cut(s) 49, 799, 1023, 1178, 1448, 1778, 1784, 2224, 2417
Hpy166II GTNNAC 7 cut(s) 406, 971, 1781, 2725, 2876, 3046, 3447
Hpy8I GTNNAC 7 cut(s) 406, 971, 1781, 2725, 2876, 3046, 3447
Hpy99I CGWCG 6 cut(s) 278, 281, 353, 398, 401, 404
HpyCH4III ACNGT 7 cut(s) 798, 975, 1905, 1961, 2374, 2571, 3052
HpyCH4IV ACGT 5 cut(s) 491, 561, 2084, 3254, 3443
HpySE526I ACGT 5 cut(s) 491, 561, 2084, 3254, 3443
HspAI GCGC 4 cut(s) 460, 462, 1598, 3420
Kpn2I TCCGGA 2 cut(s) 133, 2790
KpnI GGTACC 1 cut(s) 1199
KroI GCCGGC 1 cut(s) 2330
KroNI GCCGGC 1 cut(s) 2332
Ksp22I TGATCA 2 cut(s) 1927, 2101
KspAI GTTAAC 1 cut(s) 3447
KspI CCGCGG 1 cut(s) 373
LmnI GCTCC 9 cut(s) 67, 75, 249, 333, 823, 929, 1605, 2047, 2906
Lsp1109I GCAGC 7 cut(s) 525, 528, 1093, 1582, 2061, 3475, 3523
LweI GCATC 8 cut(s) 50, 463, 724, 779, 1745, 2858, 3307, 3500
MabI ACCWGGT 1 cut(s) 2824
MaeI CTAG 8 cut(s) 777, 1416, 1433, 1829, 2814, 2879, 3017, 3188
MaeII ACGT 5 cut(s) 491, 561, 2084, 3254, 3443
MfeI CAATTG 2 cut(s) 827, 3483
MflI RGATCY 4 cut(s) 1130, 2311, 2467, 3244
MhlI GDGCHC 5 cut(s) 72, 619, 934, 1394, 3384
MlsI TGGCCA 2 cut(s) 1983, 2016
MluNI TGGCCA 2 cut(s) 1983, 2016
MlyI GAGTC 5 cut(s) 37, 548, 1663, 2540, 2606
MmeI TCCRAC 5 cut(s) 355, 366, 978, 1699, 2264
Mox20I TGGCCA 2 cut(s) 1983, 2016
Mph1103I ATGCAT 1 cut(s) 2126
MroI TCCGGA 2 cut(s) 133, 2790
MroNI GCCGGC 1 cut(s) 2330
MroXI GAANNNNTTC 2 cut(s) 3115, 3288
MscI TGGCCA 2 cut(s) 1983, 2016
MslI CAYNNNNRTG 1 cut(s) 1973
Msp20I TGGCCA 2 cut(s) 1983, 2016
MspA1I CMGCKG 3 cut(s) 372, 1613, 1658
MspR9I CCNGG 7 cut(s) 44, 65, 317, 1866, 2022, 2826, 3024
MunI CAATTG 2 cut(s) 827, 3483
Mva1269I GAATGC 3 cut(s) 2126, 2261, 2758
MvaI CCWGG 4 cut(s) 44, 1866, 2826, 3024
MvnI CGCG 4 cut(s) 372, 462, 464, 2226
NaeI GCCGGC 1 cut(s) 2332
NciI CCSGG 3 cut(s) 65, 317, 2022
NgoMIV GCCGGC 1 cut(s) 2330
NlaIV GGNNCC 8 cut(s) 245, 329, 893, 1197, 1313, 2149, 2250, 2959
NmeAIII GCCGAG 1 cut(s) 2054
NmuCI GTSAC 4 cut(s) 304, 1899, 2230, 3052
NsiI ATGCAT 1 cut(s) 2126
NspI RCATGY 1 cut(s) 1285
PagI TCATGA 2 cut(s) 1846, 1974
PauI GCGCGC 1 cut(s) 460
PceI AGGCCT 2 cut(s) 149, 1990
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PctI GAATGC 3 cut(s) 2126, 2261, 2758
PdiI GCCGGC 1 cut(s) 2332
PdmI GAANNNNTTC 2 cut(s) 3115, 3288
PflMI CCANNNNNTGG 1 cut(s) 553
PfoI TCCNGGA 1 cut(s) 3022
Ple19I CGATCG 1 cut(s) 301
PleI GAGTC 5 cut(s) 36, 547, 1663, 2539, 2606
PpsI GAGTC 5 cut(s) 36, 547, 1663, 2539, 2606
Ppu21I YACGTR 1 cut(s) 3255
PpuMI RGGWCCY 4 cut(s) 2147, 2248, 2828, 2995
Psp124BI GAGCTC 2 cut(s) 72, 619
Psp5II RGGWCCY 4 cut(s) 2147, 2248, 2828, 2995
Psp6I CCWGG 4 cut(s) 42, 1864, 2824, 3022
PspEI GGTNACC 1 cut(s) 2230
PspFI CCCAGC 3 cut(s) 764, 1658, 3338
PspGI CCWGG 4 cut(s) 42, 1864, 2824, 3022
PspN4I GGNNCC 8 cut(s) 245, 329, 893, 1197, 1313, 2149, 2250, 2959
PspPPI RGGWCCY 4 cut(s) 2147, 2248, 2828, 2995
PsrI GAACNNNNNNTAC 2 cut(s) 677, 709
PstI CTGCAG 1 cut(s) 3130
PstNI CAGNNNCTG 1 cut(s) 2831
PsuI RGATCY 4 cut(s) 1130, 2311, 2467, 3244
PteI GCGCGC 1 cut(s) 460
PvuI CGATCG 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 1613, 1658
RsaI GTAC 7 cut(s) 1197, 1280, 1364, 1580, 1824, 2364, 2877
RsaNI GTAC 7 cut(s) 1196, 1279, 1363, 1579, 1823, 2363, 2876
RseI CAYNNNNRTG 1 cut(s) 1973
SacI GAGCTC 2 cut(s) 72, 619
SacII CCGCGG 1 cut(s) 373
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 5 cut(s) 37, 548, 1663, 2540, 2606
ScrFI CCNGG 7 cut(s) 44, 65, 317, 1866, 2022, 2826, 3024
SduI GDGCHC 5 cut(s) 72, 619, 934, 1394, 3384
SexAI ACCWGGT 1 cut(s) 2824
SfaNI GCATC 8 cut(s) 50, 463, 724, 779, 1745, 2858, 3307, 3500
SfcI CTRYAG 2 cut(s) 1041, 3126
Sfr303I CCGCGG 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 34
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 7 cut(s) 136, 1652, 2147, 2248, 2513, 2828, 2995
SmiMI CAYNNNNRTG 1 cut(s) 1973
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SpeI ACTAGT 2 cut(s) 776, 2878
SseBI AGGCCT 2 cut(s) 149, 1990
SspI AATATT 1 cut(s) 1424
SspMI CTAG 8 cut(s) 777, 1416, 1433, 1829, 2814, 2879, 3017, 3188
SstI GAGCTC 2 cut(s) 72, 619
StuI AGGCCT 2 cut(s) 149, 1990
StyD4I CCNGG 7 cut(s) 42, 63, 315, 1864, 2020, 2824, 3022
StyI CCWWGG 5 cut(s) 144, 2244, 2382, 2456, 3238
TaaI ACNGT 7 cut(s) 798, 975, 1905, 1961, 2374, 2571, 3052
TaiI ACGT 5 cut(s) 494, 564, 2087, 3257, 3446
TaqI TCGA 4 cut(s) 348, 1173, 1920, 3494
TaqII GACCGA 1 cut(s) 2665
TatI WGTACW 3 cut(s) 1278, 1578, 2875
TauI GCSGC 3 cut(s) 284, 375, 2914
TscAI CASTG 9 cut(s) 57, 313, 329, 959, 1186, 2551, 3055, 3135, 3591
TseFI GTSAC 4 cut(s) 304, 1899, 2230, 3052
TseI GCWGC 7 cut(s) 513, 516, 1106, 1595, 2074, 3488, 3536
Tsp45I GTSAC 4 cut(s) 304, 1899, 2230, 3052
TspGWI ACGGA 3 cut(s) 2480, 2492, 2535
TspRI CASTG 9 cut(s) 57, 313, 329, 959, 1186, 2551, 3055, 3135, 3591
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 7 cut(s) 136, 1652, 2147, 2248, 2513, 2828, 2995
XagI CCTNNNNNAGG 2 cut(s) 143, 2791
XapI RAATTY 6 cut(s) 840, 1142, 2213, 2562, 3067, 3303
XbaI TCTAGA 1 cut(s) 3187
XceI RCATGY 1 cut(s) 1285
XmiI GTMKAC 2 cut(s) 405, 970
XmnI GAANNNNTTC 2 cut(s) 3115, 3288
XspI CTAG 8 cut(s) 777, 1416, 1433, 1829, 2814, 2879, 3017, 3188
ZrmI AGTACT 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 2126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.