Rroxscaffold_2G00145310

trafficking protein particle complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011992
Physical Location & Seq
Reverse (-)
83224259 .. 83229951
5693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145310.1

Sequence Viewer

Length: 3555 bp
ATGGAAGAATATCCAGAGGAAATGCGGAGTCCACCGGTGAGTCTGGTCTCAGTCGTCGGCTGCTCGGAGCTCCACTCCTTCATCTCCACACACCTCCACTCCCTCGACCCTCCGATCAACACCTTGGCCTTGCCGGACCTCTCCAAGGTCTCTCTCCTCTTACTTCCCCCCAAGCCCAATCCAGCCGACTCCTCCGCCGCCGCGCCTCCTCCCGCCGGAATTTTGAAGCGCGATTGGCTTCTCAAGCACCGCACCAAAGTCCCCGCCGTTGTCGCCGCCCTCATCTCCTCGGATCGGGTCTCCGGCGACCCGGCCCAGTGGCTCGAGCTCTGCTCCGAAATCGACAATCTCAAGGGGCTCATCCGCGGCCGGAGCATCAAATTGGTCATCGTCGTCGTCAACTCCCACTCCACTGAAGAAATCTCTGAAGATCAAATGGTTGCGGTGAGGAAACGAGCTGAAGTAGATGCCAAGTATCTTCTCACGTTTTACACCGGCGAAGATTCTCAGCTTAAGCAATCTCTGCATAGATTGGGAACTGTATTTGCCGAGCTAGCCGGCATGTATTACAGAGATGAAGGAAGAAGGATTAAATTGCGCATTGAGAGGAAGAGTTCTGGTCCTACTGACTTGAACATTCGATATTCCTTCAAAGTTGCTGTGTATGCGGAGTTCAGAAGAGACTGGGTGGAAGCTTTGAGGTTTTATGAGGATGCTTATCATATGCTACGTGAGGTGATTCCCGGGGTATCAACTAGGTTGTCAGCCATTCAACGGTTAGTGGAGATAAAAACTGTGGCAGAGCAATTGCATTTCAAAATCTCAACTTTGTTATTGCATGGTGGAAAGATTATGGAAGCAGTTATGTGGTTCCGCCAACACAATGCTTCATACAGAAAGCTCAAAGGATCACCAGAAGCTATATTTCTTCACTGGGAGTGGATGAGCAGACAGTTCTTGGTGTTTGCCGAGTTACTGGAGACAAGTTCAGCAGCCATTCAGAGCATTTCACATCTAACTGCAGGCACTGCAGATAGACCTTTGACTGAGTGGGAGTTTCAGCCAGCTCATTACTATCAGTTAGCTGCTCATTATTTGAAGGAGAAGAGATCTTCTTTGGACTTTGCAGTTTCTATGTCAGAAGGTGAGATTGATTATAGTGCTGAATCTGTGGCACCCTCATCCTATATGGGTCAGTTTGCTCGATTGATTGAACAAGGAGATGCATTTGTCATGCAGCCCCTTACTGATGAAGAGTATATGCGTTATGCAATTTCTGAAGGAAAAAGGTTCCAAGATTCCTTTGAAATTATTGCTTTACTCAAAAAATCTTGTGAATCTTACAATAATCTAAAAGTCAGGAGAATGGCTTCATTTTGTGGGTTCCAGATGGCCAGAGAATATTATGCTGCAGATGATTTTAACAACGCCAAACTGCTTTTTGATGACATTGCAAGTCTATACAGGCAAGAGGGATGGGTCACTTTGCTGTGGGAGGTATTAGGTTACTTGCGTGAAGGTTCAAGGAAACACAGCAAAGTTAAAGAATTTATAGAGTACTCCTTTGAGATGGCTGCACTCCCTATATCAGCTGATACTGGGATCCAGTCTTTTAGATTTGAAGAATCTGGTCCAGCTGGGCCTGCTACCCTCCAACAAAGAGAAACAATACATAAAGAAATTTTTGGGCTTGTTAGTGAAAAACTGGGGTTGGCATCTACTGAAAACGGTGATGATGTCAAAGTAAGCGGCACCAATCCACTTCACCTTGAAATTGATCTCGTCAGTCCCCTTAGATTGGTACTCCTTGCTTCAGTTGCTTTTCATGAACAAATAATCAAGCCTGGCTCATCCACATTGGTTACACTGTCACTCTTATCACAGTTACCACTTAATTTTGAGATTGATCAATTAGAAGTCCAGTTCAATCAGTCTCATTGTAACTTTGTGATCATGGATGCCCAAAAGCGTCATGTAGCTTCCTCGACTGATGGTCAATCAGGTCACCGAAGAGAGACTGCTCCATCTCTGCGACTTTCTACAAACAAATGGCTCCGGTTAACGTATGACATCAAATCGGATCAAAGTGGAAAGCTTGAATGCATATCTATTATTGCCAAAATGGGACCGCACTTTACTATCTGTTGTAGAGCTGAAAGTCCTGCTTCAATGGATGATTTGCCTCTTTGGAAATTTGAAGACCGTGTGGTTACCTATCCAACCAAGGACCCAGCTCTGGCATTCTTCGGTCAGAGAGCAATACAAGTTGAAGAACCAGACCCAGAAGTGGATCTTACTTTAGGTGCTTCTGGCCCTGCATTAATTGGAGAGAGCTTCATAGTACCTGTTACTGTCACATCCAAGGGACATGAGGTTAATTCGGGTGAGTTGAAGATCAATCTGGTGGATGTAAGGGGAGGTGGTTTGTTTAGTCCAAGGGATGCAGAACTATCTGCGGATAGTCATCATGTTGAACTTCTTGGTGTATCTGGATCAGAAGGAGAAGATGAATCCCAATTGGACACCGATGAAATTAAGAAAATTCAAAAGTCTTTTGGATTAGTTTCAGTTCCTAATCTCAGAAGTGGAGACTCATGGTCATGCAAATTGGAAATCAAGTGGTATAGACCCAAACCAGTTATGCTTTATGTATCACTGGGTTATTCTCCAGATAATAATGGATCAAACACACAAAAAGTTAATGTGCACAAGAGCTTGCAGATTGAAGGAAAGAATGCAATCTCAATTAGCCATCGCCTTATGCTTCCATTCCGCAGGTACCCATTGTTGCTTTCTAGGACAAAACCAGTTCCTGATTCTGATCAGTCAGTGTCAATGCCTTTAAACGAAACAAGTGTACTCATTGTTAGTGCTACGAATTGCTCAGAGGTGCCACTTCAGTTACTATCATTATCTATTGAAAGAGATAATGATGACACTGAAAGGTCATGTTCTTTGCATGGAGGTGAGGACCTTTTAAACCCTGCCCTTCTTGTTCCAGGAGAGAAGTTTAAGAAGGTGTACACTGTCACTTCTGAGATGAATTCTTCAAAGCTTATATTAGGTAATGTGTGTCTTAGATGGAGAAGGAATTCTGGGACTAGAGAACAGGCTGGTTCTGCGGCTCCAGTTATGACTAACCACAAACTTCCTGATGTGAATCTGGAATCGTCACCTCTTGTCGTGAGTTTGGAGTGTCCTCCTTACGCAATCCTTGGCGATCCTTTCACATATTTTGTTAAAATTCAGAACCAAACAGAACTGCTTCAAGAGGCCAAAATTTTCCTGGCAGATGCTCAAAGTTTTGTAATATCTGGGTCTCACAGTGACACGGTATATATTCTTCCAAAATCTGAGCACATCATCAGTTACAAGCTTGTCCCGCTTTCCTCAGGGGAGCAACAGCTACCCAGATTCACTTTAACCGCAGTTAGATATTCCACTGGGTTTCAGCCTTCAATTGCTGCATCGACTATTTTTGTTTTCCCCTCTCAGCCTCATTTTAAGATGGTTGCTGTGGGAGACGACCGGAAGGAATCAGTGGTGGCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Foie-gras_1 Gryzun-like

Protein Analysis

1184

Amino Acids

132.28

Weight (kDa)

5.84

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Foie-gras_1 PF11817 259 - 526 6.2e-67 Foie gras liver health family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011992)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65950
fragaria_vesca FvH4_1g10000
malus_domestica MD15G1228100.v1.1
prunus_persica Prupe.7G186800_v2.0.a1 Prupe.7G186800_v2.0.a1
pyrus_communis pycom15g20290
rosa_chinensis RchiOBHm_Chr2g0096791
rosa_laevigata RLG00000016670 RLG00000023097
rosa_multiflora Rmu_co8505723.1_g000001 Rmu_sc0004938.1_g000018 Rmu_ssc0000215.1_g000031
rosa_roxburghii Rroxscaffold_2G00145310
rosa_rugosa Rorug02G0060900
rosa_samantha Rh2AG108200 Rh2BG110400 Rh2CG112300 Rh2DG111700
rosa_wichuraiana Rw2G008370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 2766
Acc65I GGTACC 1 cut(s) 2778
AccB1I GGYRCC 4 cut(s) 1174, 1751, 2778, 2890
AccII CGCG 3 cut(s) 203, 231, 366
AclWI GGATC 9 cut(s) 300, 916, 1597, 1610, 2088, 2298, 2500, 2689, 3215
AcoI YGGCCR 2 cut(s) 367, 1392
AcsI RAATTY 9 cut(s) 219, 1547, 1680, 2192, 2541, 3043, 3091, 3243, 3279
AcuI CTGAAG 6 cut(s) 435, 447, 480, 1299, 1797, 2882
AfaI GTAC 6 cut(s) 1559, 1803, 2343, 2780, 2859, 3023
AfiI CCNNNNNNNGG 7 cut(s) 145, 215, 294, 774, 1798, 2236, 2287
AflII CTTAAG 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 34
AhdI GACNNNNNGTC 2 cut(s) 1992, 2596
AjnI CCWGG 3 cut(s) 1843, 2998, 3285
Alw21I GWGCWC 4 cut(s) 72, 330, 2709, 3360
Alw44I GTGCAC 1 cut(s) 2705
AlwI GGATC 9 cut(s) 300, 916, 1597, 1610, 2088, 2298, 2500, 2689, 3215
AlwNI CAGNNNCTG 2 cut(s) 1028, 2813
Ama87I CYCGRG 2 cut(s) 323, 743
AoxI GGCC 7 cut(s) 126, 312, 367, 1392, 1640, 2311, 3273
ApaLI GTGCAC 1 cut(s) 2705
ApeKI GCWGC 7 cut(s) 60, 992, 1085, 1237, 1409, 1574, 3464
ApoI RAATTY 9 cut(s) 219, 1547, 1680, 2192, 2541, 3043, 3091, 3243, 3279
AseI ATTAAT 1 cut(s) 2321
AsiGI ACCGGT 1 cut(s) 34
Asp700I GAANNNNTTC 3 cut(s) 1369, 3091, 3264
Asp718I GGTACC 1 cut(s) 2778
AspLEI GCGC 3 cut(s) 205, 231, 600
AspS9I GGNCC 9 cut(s) 136, 313, 620, 1631, 1640, 2126, 2227, 2312, 2971
AsuC2I CCSGG 3 cut(s) 311, 744, 745
AsuNHI GCTAGC 1 cut(s) 553
AvaI CYCGRG 2 cut(s) 323, 743
AvaII GGWCC 6 cut(s) 136, 620, 1631, 2126, 2227, 2971
AxyI CCTNAGG 1 cut(s) 3391
BaeGI GKGCMC 1 cut(s) 2709
BalI TGGCCA 1 cut(s) 1394
BamHI GGATCC 1 cut(s) 1602
BanI GGYRCC 4 cut(s) 1174, 1751, 2778, 2890
BanII GRGCYC 3 cut(s) 72, 330, 360
BarI GAAGNNNNNNTAC 2 cut(s) 3327, 3359
BbsI GAAGAC 1 cut(s) 2205
Bbv12I GWGCWC 4 cut(s) 72, 330, 2709, 3360
BbvI GCAGC 7 cut(s) 47, 1004, 1072, 1249, 1396, 1561, 3451
BccI CCATC 8 cut(s) 1384, 1470, 1564, 1985, 2032, 2760, 3075, 3502
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 3 cut(s) 1845, 3000, 3287
BclI TGATCA 3 cut(s) 1906, 1950, 2821
BcnI CCSGG 3 cut(s) 311, 744, 745
BfaI CTAG 4 cut(s) 554, 756, 2796, 3102
BfmI CTRYAG 3 cut(s) 1020, 1029, 1410
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 2766
BglII AGATCT 1 cut(s) 1109
BmcAI AGTACT 1 cut(s) 1559
Bme1390I CCNGG 6 cut(s) 311, 744, 745, 1845, 3000, 3287
Bme18I GGWCC 6 cut(s) 136, 620, 1631, 2126, 2227, 2971
BmeRI GACNNNNNGTC 2 cut(s) 1992, 2596
BmeT110I CYCGRG 2 cut(s) 323, 743
BmgT120I GGNCC 9 cut(s) 136, 313, 620, 1631, 1640, 2126, 2227, 2312, 2971
BmrFI CCNGG 6 cut(s) 311, 744, 745, 1845, 3000, 3287
BmrI ACTGGG 7 cut(s) 310, 694, 943, 1608, 1715, 2666, 3453
BmsI GCATC 9 cut(s) 384, 457, 703, 1213, 1724, 1948, 2431, 3283, 3476
BmtI GCTAGC 1 cut(s) 557
BmuI ACTGGG 7 cut(s) 310, 694, 943, 1608, 1715, 2666, 3453
BpiI GAAGAC 1 cut(s) 2205
BplI GAGNNNNNCTC 4 cut(s) 59, 91, 317, 349
BpmI CTGGAG 3 cut(s) 998, 2652, 3111
BpuEI CTTGAG 2 cut(s) 227, 335
BpuMI CCSGG 3 cut(s) 311, 744, 745
BsaAI YACGTR 1 cut(s) 731
BsaBI GATNNNNATC 4 cut(s) 717, 2463, 2820, 3159
BsaI GGTCTC 4 cut(s) 52, 154, 304, 3324
BsaWI WCCGGW 3 cut(s) 34, 2055, 3528
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 392, 422
Bsc4I CCNNNNNNNGG 7 cut(s) 145, 215, 294, 774, 1798, 2236, 2287
Bse118I RCCGGY 3 cut(s) 34, 494, 557
Bse21I CCTNAGG 1 cut(s) 3391
Bse3DI GCAATG 1 cut(s) 1449
Bse8I GATNNNNATC 4 cut(s) 717, 2463, 2820, 3159
BseBI CCWGG 3 cut(s) 1845, 3000, 3287
BseJI GATNNNNATC 4 cut(s) 717, 2463, 2820, 3159
BseLI CCNNNNNNNGG 7 cut(s) 145, 215, 294, 774, 1798, 2236, 2287
BseMI GCAATG 1 cut(s) 1449
BseMII CTCAG 9 cut(s) 63, 521, 1038, 2593, 2898, 3027, 3345, 3405, 3506
BseRI GAGGAG 4 cut(s) 146, 181, 198, 277
BseSI GKGCMC 1 cut(s) 2709
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 7 cut(s) 47, 1004, 1072, 1249, 1396, 1561, 3451
BseYI CCCAGC 2 cut(s) 1637, 2230
BsgI GTGCAG 1 cut(s) 1560
Bsh1236I CGCG 3 cut(s) 203, 231, 366
Bsh1285I CGRYCG 2 cut(s) 370, 3529
BshFI GGCC 7 cut(s) 128, 314, 369, 1394, 1642, 2313, 3275
BshNI GGYRCC 4 cut(s) 1174, 1751, 2778, 2890
BshTI ACCGGT 1 cut(s) 34
BsiEI CGRYCG 2 cut(s) 370, 3529
BsiHKAI GWGCWC 4 cut(s) 72, 330, 2709, 3360
BsiHKCI CYCGRG 2 cut(s) 323, 743
BslFI GGGAC 6 cut(s) 245, 1773, 2139, 2379, 3112, 3365
BslI CCNNNNNNNGG 7 cut(s) 145, 215, 294, 774, 1798, 2236, 2287
BsmBI CGTCTC 1 cut(s) 3516
BsmFI GGGAC 6 cut(s) 245, 1773, 2139, 2379, 3112, 3365
BsmI GAATGC 3 cut(s) 2105, 2240, 2740
BsnI GGCC 7 cut(s) 128, 314, 369, 1394, 1642, 2313, 3275
Bso31I GGTCTC 4 cut(s) 52, 154, 304, 3324
BsoBI CYCGRG 2 cut(s) 323, 743
Bsp1286I GDGCHC 5 cut(s) 72, 330, 360, 2709, 3360
Bsp1407I TGTACA 1 cut(s) 3021
BspANI GGCC 7 cut(s) 128, 314, 369, 1394, 1642, 2313, 3275
BspCNI CTCAG 9 cut(s) 62, 520, 1039, 2592, 2897, 3028, 3346, 3404, 3505
BspFNI CGCG 3 cut(s) 203, 231, 366
BspHI TCATGA 1 cut(s) 1825
BspMAI CTGCAG 3 cut(s) 1024, 1033, 1414
BspMI ACCTGC 1 cut(s) 2766
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 9 cut(s) 300, 916, 1597, 1610, 2088, 2298, 2500, 2689, 3215
BspT107I GGYRCC 4 cut(s) 1174, 1751, 2778, 2890
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 4 cut(s) 52, 154, 304, 3324
BsrDI GCAATG 1 cut(s) 1449
BsrFI RCCGGY 3 cut(s) 34, 494, 557
BsrGI TGTACA 1 cut(s) 3021
BssAI RCCGGY 3 cut(s) 34, 494, 557
BssT1I CCWWGG 6 cut(s) 123, 144, 2223, 2361, 2435, 3214
Bst2UI CCWGG 3 cut(s) 1845, 3000, 3287
Bst6I CTCTTC 5 cut(s) 605, 673, 1100, 1248, 2005
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 2 cut(s) 523, 1028
BstAUI TGTACA 1 cut(s) 3021
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 6 cut(s) 555, 559, 1024, 1065, 1644, 2717
BstDSI CCRYGG 1 cut(s) 364
BstEII GGTNACC 2 cut(s) 2003, 2209
BstENI CCTNNNNNAGG 1 cut(s) 143
BstFNI CGCG 3 cut(s) 203, 231, 366
BstHHI GCGC 3 cut(s) 205, 231, 600
BstMCI CGRYCG 2 cut(s) 370, 3529
BstNI CCWGG 3 cut(s) 1845, 3000, 3287
BstNSI RCATGY 1 cut(s) 565
BstPI GGTNACC 2 cut(s) 2003, 2209
BstSCI CCNGG 6 cut(s) 309, 742, 743, 1843, 2998, 3285
BstSFI CTRYAG 3 cut(s) 1020, 1029, 1410
BstSLI GKGCMC 1 cut(s) 2709
BstUI CGCG 3 cut(s) 203, 231, 366
BstV1I GCAGC 7 cut(s) 47, 1004, 1072, 1249, 1396, 1561, 3451
BstV2I GAAGAC 1 cut(s) 2205
BstX2I RGATCY 3 cut(s) 1109, 1602, 2290
BstYI RGATCY 3 cut(s) 1109, 1602, 2290
BstZI CGGCCG 1 cut(s) 367
Bsu36I CCTNAGG 1 cut(s) 3391
BsuRI GGCC 7 cut(s) 128, 314, 369, 1394, 1642, 2313, 3275
BtgI CCRYGG 1 cut(s) 364
BtgZI GCGATG 1 cut(s) 2738
BtsI GCAGTG 1 cut(s) 1026
BveI ACCTGC 1 cut(s) 2766
Cac8I GCNNGC 6 cut(s) 555, 559, 1024, 1065, 1644, 2717
CaiI CAGNNNCTG 2 cut(s) 1028, 2813
CciI TCATGA 1 cut(s) 1825
CfoI GCGC 3 cut(s) 205, 231, 600
Cfr10I RCCGGY 3 cut(s) 34, 494, 557
Cfr13I GGNCC 9 cut(s) 136, 313, 620, 1631, 1640, 2126, 2227, 2312, 2971
Cfr42I CCGCGG 1 cut(s) 367
Cfr9I CCCGGG 1 cut(s) 743
CseI GACGC 1 cut(s) 1958
Csp6I GTAC 6 cut(s) 1558, 1802, 2342, 2779, 2858, 3022
CspAI ACCGGT 1 cut(s) 34
CviQI GTAC 6 cut(s) 1558, 1802, 2342, 2779, 2858, 3022
DraI TTTAAA 2 cut(s) 2844, 2979
DriI GACNNNNNGTC 2 cut(s) 1992, 2596
EaeI YGGCCR 2 cut(s) 367, 1392
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 5 cut(s) 605, 673, 1100, 1248, 2005
Eam1105I GACNNNNNGTC 2 cut(s) 1992, 2596
EarI CTCTTC 5 cut(s) 605, 673, 1100, 1248, 2005
EciI GGCGGA 2 cut(s) 184, 863
Ecl136II GAGCTC 2 cut(s) 70, 328
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 6 cut(s) 123, 144, 2223, 2361, 2435, 3214
Eco24I GRGCYC 3 cut(s) 72, 330, 360
Eco31I GGTCTC 4 cut(s) 52, 154, 304, 3324
Eco47I GGWCC 6 cut(s) 136, 620, 1631, 2126, 2227, 2971
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 2 cut(s) 70, 328
Eco57I CTGAAG 6 cut(s) 435, 447, 480, 1299, 1797, 2882
Eco81I CCTNAGG 1 cut(s) 3391
Eco88I CYCGRG 2 cut(s) 323, 743
Eco91I GGTNACC 2 cut(s) 2003, 2209
EcoICRI GAGCTC 2 cut(s) 70, 328
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO109I RGGNCCY 2 cut(s) 2227, 2971
EcoO65I GGTNACC 2 cut(s) 2003, 2209
EcoRI GAATTC 2 cut(s) 3043, 3091
EcoRII CCWGG 3 cut(s) 1843, 2998, 3285
EcoT14I CCWWGG 6 cut(s) 123, 144, 2223, 2361, 2435, 3214
EcoT22I ATGCAT 2 cut(s) 1228, 2105
EcoT38I GRGCYC 3 cut(s) 72, 330, 360
ErhI CCWWGG 6 cut(s) 123, 144, 2223, 2361, 2435, 3214
Esp3I CGTCTC 1 cut(s) 3516
FalI AAGNNNNNCTT 4 cut(s) 2149, 2181, 2277, 2309
FaqI GGGAC 6 cut(s) 245, 1773, 2139, 2379, 3112, 3365
FauI CCCGC 3 cut(s) 220, 271, 3390
FauNDI CATATG 1 cut(s) 723
FbaI TGATCA 3 cut(s) 1906, 1950, 2821
FriOI GRGCYC 3 cut(s) 72, 330, 360
FspBI CTAG 4 cut(s) 554, 756, 2796, 3102
FspI TGCGCA 1 cut(s) 599
GlaI GCGC 3 cut(s) 204, 230, 599
GsaI CCCAGC 2 cut(s) 1641, 2234
GsuI CTGGAG 3 cut(s) 998, 2652, 3111
HaeIII GGCC 7 cut(s) 128, 314, 369, 1394, 1642, 2313, 3275
HgaI GACGC 1 cut(s) 1958
HhaI GCGC 3 cut(s) 205, 231, 600
Hin6I GCGC 3 cut(s) 203, 229, 598
HinP1I GCGC 3 cut(s) 203, 229, 598
HincII GTYRAC 2 cut(s) 400, 2061
HindII GTYRAC 2 cut(s) 400, 2061
HindIII AAGCTT 4 cut(s) 693, 2093, 3053, 3374
HpaI GTTAAC 1 cut(s) 2061
Hpy166II GTNNAC 7 cut(s) 32, 400, 2061, 2707, 2858, 3022, 3024
Hpy8I GTNNAC 7 cut(s) 32, 400, 2061, 2707, 2858, 3022, 3024
Hpy99I CGWCG 3 cut(s) 59, 395, 398
HpyCH4IV ACGT 3 cut(s) 485, 730, 2063
HpySE526I ACGT 3 cut(s) 485, 730, 2063
HspAI GCGC 3 cut(s) 203, 229, 598
KpnI GGTACC 1 cut(s) 2782
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Ksp22I TGATCA 3 cut(s) 1906, 1950, 2821
KspAI GTTAAC 1 cut(s) 2061
KspI CCGCGG 1 cut(s) 367
LmnI GCTCC 8 cut(s) 67, 75, 338, 372, 2026, 2058, 3130, 3397
Lsp1109I GCAGC 7 cut(s) 47, 1004, 1072, 1249, 1396, 1561, 3451
LweI GCATC 9 cut(s) 384, 457, 703, 1213, 1724, 1948, 2431, 3283, 3476
MaeI CTAG 4 cut(s) 554, 756, 2796, 3102
MaeII ACGT 3 cut(s) 485, 730, 2063
MfeI CAATTG 3 cut(s) 806, 2516, 3459
MflI RGATCY 3 cut(s) 1109, 1602, 2290
MhlI GDGCHC 5 cut(s) 72, 330, 360, 2709, 3360
MlsI TGGCCA 1 cut(s) 1394
MluNI TGGCCA 1 cut(s) 1394
MlyI GAGTC 4 cut(s) 37, 49, 182, 2585
MmeI TCCRAC 2 cut(s) 1678, 2243
Mox20I TGGCCA 1 cut(s) 1394
Mph1103I ATGCAT 2 cut(s) 1228, 2105
MroNI GCCGGC 1 cut(s) 557
MroXI GAANNNNTTC 3 cut(s) 1369, 3091, 3264
MscI TGGCCA 1 cut(s) 1394
MslI CAYNNNNRTG 2 cut(s) 2599, 2964
Msp20I TGGCCA 1 cut(s) 1394
MspA1I CMGCKG 3 cut(s) 366, 1592, 1637
MspCI CTTAAG 1 cut(s) 512
MspR9I CCNGG 6 cut(s) 311, 744, 745, 1845, 3000, 3287
MunI CAATTG 3 cut(s) 806, 2516, 3459
Mva1269I GAATGC 3 cut(s) 2105, 2240, 2740
MvaI CCWGG 3 cut(s) 1845, 3000, 3287
MvnI CGCG 3 cut(s) 203, 231, 366
NaeI GCCGGC 1 cut(s) 559
NciI CCSGG 3 cut(s) 311, 744, 745
NdeI CATATG 1 cut(s) 723
NgoMIV GCCGGC 1 cut(s) 557
NheI GCTAGC 1 cut(s) 553
NmeAIII GCCGAG 2 cut(s) 574, 994
NmuCI GTSAC 7 cut(s) 1480, 1869, 2003, 2353, 3028, 3171, 3326
NsbI TGCGCA 1 cut(s) 599
NsiI ATGCAT 2 cut(s) 1228, 2105
NspI RCATGY 1 cut(s) 565
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 1 cut(s) 1825
PctI GAATGC 3 cut(s) 2105, 2240, 2740
PdiI GCCGGC 1 cut(s) 559
PdmI GAANNNNTTC 3 cut(s) 1369, 3091, 3264
PfoI TCCNGGA 1 cut(s) 2998
PinAI ACCGGT 1 cut(s) 34
PleI GAGTC 4 cut(s) 36, 48, 182, 2585
PpsI GAGTC 4 cut(s) 36, 48, 182, 2585
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 2 cut(s) 2227, 2971
PshBI ATTAAT 1 cut(s) 2321
Psp124BI GAGCTC 2 cut(s) 72, 330
Psp5II RGGWCCY 2 cut(s) 2227, 2971
Psp6I CCWGG 3 cut(s) 1843, 2998, 3285
PspEI GGTNACC 2 cut(s) 2003, 2209
PspFI CCCAGC 2 cut(s) 1637, 2230
PspGI CCWGG 3 cut(s) 1843, 2998, 3285
PspPI GGNCC 9 cut(s) 136, 313, 620, 1631, 1640, 2126, 2227, 2312, 2971
PspPPI RGGWCCY 2 cut(s) 2227, 2971
PspXI VCTCGAGB 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 656, 688
PstI CTGCAG 3 cut(s) 1024, 1033, 1414
PstNI CAGNNNCTG 2 cut(s) 1028, 2813
PsuI RGATCY 3 cut(s) 1109, 1602, 2290
PvuII CAGCTG 2 cut(s) 1592, 1637
RsaI GTAC 6 cut(s) 1559, 1803, 2343, 2780, 2859, 3023
RsaNI GTAC 6 cut(s) 1558, 1802, 2342, 2779, 2858, 3022
RseI CAYNNNNRTG 2 cut(s) 2599, 2964
SacI GAGCTC 2 cut(s) 72, 330
SacII CCGCGG 1 cut(s) 367
Sau96I GGNCC 9 cut(s) 136, 313, 620, 1631, 1640, 2126, 2227, 2312, 2971
ScaI AGTACT 1 cut(s) 1559
SchI GAGTC 4 cut(s) 37, 49, 182, 2585
ScrFI CCNGG 6 cut(s) 311, 744, 745, 1845, 3000, 3287
SduI GDGCHC 5 cut(s) 72, 330, 360, 2709, 3360
SfaNI GCATC 9 cut(s) 384, 457, 703, 1213, 1724, 1948, 2431, 3283, 3476
SfcI CTRYAG 3 cut(s) 1020, 1029, 1410
Sfr274I CTCGAG 1 cut(s) 323
Sfr303I CCGCGG 1 cut(s) 367
SgrAI CRCCGGYG 2 cut(s) 34, 494
SgrBI CCGCGG 1 cut(s) 367
SinI GGWCC 6 cut(s) 136, 620, 1631, 2126, 2227, 2971
SlaI CTCGAG 1 cut(s) 323
SmaI CCCGGG 1 cut(s) 745
SmiMI CAYNNNNRTG 2 cut(s) 2599, 2964
SmlI CTYRAG 4 cut(s) 242, 323, 350, 512
SmoI CTYRAG 4 cut(s) 242, 323, 350, 512
SspI AATATT 1 cut(s) 1403
SspMI CTAG 4 cut(s) 554, 756, 2796, 3102
SstI GAGCTC 2 cut(s) 72, 330
StyD4I CCNGG 6 cut(s) 309, 742, 743, 1843, 2998, 3285
StyI CCWWGG 6 cut(s) 123, 144, 2223, 2361, 2435, 3214
TaiI ACGT 3 cut(s) 488, 733, 2066
TaqI TCGA 7 cut(s) 105, 324, 342, 640, 1204, 1985, 3470
TaqII GACCGA 1 cut(s) 2237
TatI WGTACW 3 cut(s) 1557, 2857, 3021
TauI GCSGC 6 cut(s) 200, 203, 278, 369, 1752, 3125
TseFI GTSAC 7 cut(s) 1480, 1869, 2003, 2353, 3028, 3171, 3326
TseI GCWGC 7 cut(s) 60, 992, 1085, 1237, 1409, 1574, 3464
Tsp45I GTSAC 7 cut(s) 1480, 1869, 2003, 2353, 3028, 3171, 3326
TspMI CCCGGG 1 cut(s) 743
Vha464I CTTAAG 1 cut(s) 512
VneI GTGCAC 1 cut(s) 2705
VpaK11BI GGWCC 6 cut(s) 136, 620, 1631, 2126, 2227, 2971
VspI ATTAAT 1 cut(s) 2321
XagI CCTNNNNNAGG 1 cut(s) 143
XapI RAATTY 9 cut(s) 219, 1547, 1680, 2192, 2541, 3043, 3091, 3243, 3279
XceI RCATGY 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 3283
XhoI CTCGAG 1 cut(s) 323
XmaI CCCGGG 1 cut(s) 743
XmnI GAANNNNTTC 3 cut(s) 1369, 3091, 3264
XspI CTAG 4 cut(s) 554, 756, 2796, 3102
ZrmI AGTACT 1 cut(s) 1559
Zsp2I ATGCAT 2 cut(s) 1228, 2105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.