Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001957

Physical Location & Seq

Genomic Coordinates Reverse (-)

48498096 .. 48504769

Gene Structure 6674 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1390500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCGCTTCTCAGGCTCTCGAGCTCCATCAACAAGAAGCTGCCCTTTCAACCTTTCTTCAATGGCGGCACCATATACCACATGTCCTCTCTGGCCAGCCAGGCTGCTCAAGACAAAGGGAAAGCTCGCACCACTTGGATTAAGCAGTTGAATGAGCCGCTTGAGGCTGTCGATTCTGAGATTGCCGATATCATCGAGCTCGAAAAAGCTCGGCAATGGAAGGGGCTTGAGCTTATACCATCAGAAAATTTCACGTCACTTTCGGTGATGCAAGCTGTTGGGTCCGTCATGACCAACAAGTACAGTGAAGGGTATCCTGGTGCTAGATACTATGGAGGAAATGAATACATCGACATGGCTGAGTCCCTGTGTCAAAAACGTGCATTAGAAGCCTTCCAGTTAGATCCTGCAAAATGGGGAGTGAATGTGCAGCCACTATCTGGATCCCCTGCAAACTTCCAAGCTTATACTGCATTGTTGAAACCTCATGAAAGAATCATGGCACTTGATCTACCTCACGGCGGACATCTTTCTCATGGTTATCAGACTGACACCAAAAAGATATCTGCTGTGTCGATATTTTTTGAGACAATGCCATACAGATTGAATGAGAGCACTGGCTACATTGATTATGATCAGATGGAGAAAAGTGCCACACTTTTCAGGCCAAAGCTAATAGTTGCTGGTGCAAGTGCTTATGCACGCCTGTACGATTATGAGCGTATCCGCAAGGTCTGTGATAAGCAGAAAGCAATTCTGTTGGCTGATATGGCACACATCAGTGGATTGGTTGCTGCCGATGTTATTCCATCTCCTTTTGAGTATGCAGATATTGTTACAACCACAACTCATAAGTCACTTCGTGGACCTCGTGGGGCTATGATCTTCTTCAGGAAGGGTGTCAAGCATATAAACAAGCAAGGGCAAGAAGTTATGTATGATTATGAAGACAAAATCAATCAGGCTGTCTTTCCTGGACTCCAGGGTGGTCCGCACAACCACACCATAGCAGGGCTAGCAGTTGCACTGAAGCAGGTCAAAACTCCGGAGTACAAAGCTTATCAGGAACAAGTTCTCAGTAATTGCTCAAAATTTGCAGAGAGCTTGCTAGAGAAGGGCTATGATCTTGTTTCCGGTGGAACAGAGAACCATTTAGTGTTGGTGAATTTAAGAGACAAGGGCATTGATGGCTCTAGAGTTGAAAAAGTTTTGGAATCAGCACATATTGCAGCAAATAAAAATACTGTTCCTGGGGACGTGTCTGCCATGGTCCCTGGTGGCATACGCATGGGAACCCCAGCTCTTACGTCTAGGGGGTTTTTGGAGGAGGATTTTGTGAAAGTAGCTGAGTATTTTGATGCAGCTGTAAAGTTGGCCTTGAAGATCAAGGCTGCTGCCAAAGGTACGAAGTTGAAGGATTTCGTGGCAGTTCTGGAATCAGACGGTGAAGTTCAGTCTGAGATTACAAAGCTTCGCCACGAGGTTGAGCAGTACGCTAAACAGTTTCCAACCATTGGATTCGAGAAAGAAACAATGAGATACAGAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

517

Amino Acids

57.23

Weight (kDa)

7.69

Isoelectric Point (pI)

35.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	54 - 451	4e-212	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26780 AT5G26780 AT5G26780 AT5G26780
fragaria_vesca	FvH4_5g34000 FvH4_5g34000 FvH4_5g34000
malus_domestica	MD08G1206800.v1.1 MD15G1390500.v1.1
prunus_persica	Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1
pyrus_communis	pycom08g17860
rosa_chinensis	RchiOBHm_Chr5g0052761 RchiOBHm_Chr7g0233441 RchiOBHm_Chr7g0235741
rosa_laevigata	RLG00000001270 RLG00000013832 RLG00000035486
rosa_multiflora	Rmu_sc0000029.1_g000053 Rmu_sc0006510.1_g000006
rosa_roxburghii	Rroxscaffold_3G00227300 Rroxscaffold_3G00241170
rosa_rugosa	Rorug07G0280700
rosa_samantha	Rh1CG020600 Rh1CG022300 Rh2AG156000 Rh2BG162300 Rh2BG221600 Rh2DG161400 Rh4DG362000 Rh5BG352800 Rh5CG247000 Rh5CG419500 Rh5DG369100 Rh7AG154700 Rh7AG436500 Rh7AG488000 Rh7BG409600 Rh7CG456400 Rh7DG425700
rosa_wichuraiana	Rw0G022490 Rw7G035990 Rw7G036020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1024
AccB1I	GGYRCC	1 cut(s)	68
AccB7I	CCANNNNNTGG	2 cut(s)	440, 1514
AccIII	TCCGGA	1 cut(s)	1045
AciI	CCGC	5 cut(s)	66, 158, 522, 727, 992
AclWI	GGATC	3 cut(s)	398, 438, 451
AcoI	YGGCCR	1 cut(s)	93
AcsI	RAATTY	3 cut(s)	247, 1091, 1165
AcuI	CTGAAG	2 cut(s)	874, 1049
AdeI	CACNNNGTG	1 cut(s)	863
AfaI	GTAC	5 cut(s)	302, 710, 1052, 1405, 1493
AfiI	CCNNNNNNNGG	4 cut(s)	440, 521, 1011, 1514
AflIII	ACRYGT	2 cut(s)	81, 1257
AgsI	TTSAA	8 cut(s)	50, 61, 151, 481, 607, 1202, 1381, 1414
AjiI	CACGTC	2 cut(s)	255, 1258
AjnI	CCWGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
AleI	CACNNNNGTG	1 cut(s)	780
Alw21I	GWGCWC	3 cut(s)	26, 201, 617
Alw26I	GTCTC	2 cut(s)	581, 1167
AlwI	GGATC	3 cut(s)	398, 438, 451
Ama87I	CYCGRG	1 cut(s)	19
Aor13HI	TCCGGA	1 cut(s)	1045
AoxI	GGCC	3 cut(s)	93, 665, 1374
ApeKI	GCWGC	8 cut(s)	40, 104, 430, 794, 1229, 1361, 1391, 1394
ApoI	RAATTY	3 cut(s)	247, 1091, 1165
Asp700I	GAANNNNTTC	2 cut(s)	1071, 1418
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	4 cut(s)	282, 866, 989, 1270
AsuHPI	GGTGA	3 cut(s)	277, 1174, 1457
AsuNHI	GCTAGC	1 cut(s)	1015
AvaI	CYCGRG	1 cut(s)	19
AvaII	GGWCC	4 cut(s)	282, 866, 989, 1270
BalI	TGGCCA	1 cut(s)	95
BamHI	GGATCC	1 cut(s)	443
BanI	GGYRCC	1 cut(s)	68
BanII	GRGCYC	2 cut(s)	26, 201
BauI	CACGAG	2 cut(s)	870, 1478
BbsI	GAAGAC	1 cut(s)	954
Bbv12I	GWGCWC	3 cut(s)	26, 201, 617
BbvI	GCAGC	8 cut(s)	27, 91, 442, 781, 1241, 1373, 1378, 1381
BccI	CCATC	5 cut(s)	35, 247, 634, 817, 1181
BceAI	ACGGC	1 cut(s)	535
BciT130I	CCWGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
BciVI	GTATCC	2 cut(s)	324, 734
BclI	TGATCA	1 cut(s)	634
BcoDI	GTCTC	2 cut(s)	581, 1167
BfaI	CTAG	5 cut(s)	324, 1016, 1109, 1194, 1311
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	1024
BfuI	GTATCC	2 cut(s)	324, 734
BglI	GCCNNNNNGGC	1 cut(s)	101
Bme1390I	CCNGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
Bme18I	GGWCC	4 cut(s)	282, 866, 989, 1270
BmeT110I	CYCGRG	1 cut(s)	19
BmgBI	CACGTC	2 cut(s)	255, 1258
BmgT120I	GGNCC	4 cut(s)	282, 866, 989, 1270
BmiI	GGNNCC	5 cut(s)	70, 283, 445, 1272, 1294
BmrFI	CCNGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
BmsI	GCATC	2 cut(s)	258, 1348
BmtI	GCTAGC	1 cut(s)	1019
BpiI	GAAGAC	1 cut(s)	954
BpmI	CTGGAG	1 cut(s)	965
BpuEI	CTTGAG	3 cut(s)	93, 182, 248
BsaBI	GATNNNNATC	1 cut(s)	633
BsaJI	CCNNGG	4 cut(s)	982, 1250, 1266, 1273
BsaWI	WCCGGW	2 cut(s)	1045, 1133
BsaXI	ACNNNNNCTCC	2 cut(s)	411, 441
Bsc4I	CCNNNNNNNGG	4 cut(s)	440, 521, 1011, 1514
Bse1I	ACTGG	2 cut(s)	397, 622
Bse3DI	GCAATG	1 cut(s)	221
Bse8I	GATNNNNATC	1 cut(s)	633
BseAI	TCCGGA	1 cut(s)	1045
BseBI	CCWGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
BseDI	CCNNGG	4 cut(s)	982, 1250, 1266, 1273
BseJI	GATNNNNATC	1 cut(s)	633
BseLI	CCNNNNNNNGG	4 cut(s)	440, 521, 1011, 1514
BseMI	GCAATG	1 cut(s)	221
BseMII	CTCAG	6 cut(s)	25, 168, 351, 1090, 1338, 1449
BseNI	ACTGG	2 cut(s)	397, 622
BseRI	GAGGAG	1 cut(s)	1340
BseXI	GCAGC	8 cut(s)	27, 91, 442, 781, 1241, 1373, 1378, 1381
BseYI	CCCAGC	1 cut(s)	1297
BsgI	GTGCAG	1 cut(s)	449
BshFI	GGCC	3 cut(s)	95, 667, 1376
BshNI	GGYRCC	1 cut(s)	68
BsiHKAI	GWGCWC	3 cut(s)	26, 201, 617
BsiHKCI	CYCGRG	1 cut(s)	19
BsiSI	CCGG	2 cut(s)	1046, 1134
BslFI	GGGAC	3 cut(s)	349, 1256, 1268
BslI	CCNNNNNNNGG	4 cut(s)	440, 521, 1011, 1514
BsmAI	GTCTC	2 cut(s)	581, 1167
BsmFI	GGGAC	3 cut(s)	349, 1256, 1268
BsnI	GGCC	3 cut(s)	95, 667, 1376
BsoBI	CYCGRG	1 cut(s)	19
Bsp1286I	GDGCHC	3 cut(s)	26, 201, 617
Bsp13I	TCCGGA	1 cut(s)	1045
Bsp143I	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
Bsp19I	CCATGG	1 cut(s)	1266
BspACI	CCGC	5 cut(s)	66, 158, 522, 727, 992
BspANI	GGCC	3 cut(s)	95, 667, 1376
BspCNI	CTCAG	6 cut(s)	24, 169, 352, 1089, 1339, 1450
BspEI	TCCGGA	1 cut(s)	1045
BspHI	TCATGA	2 cut(s)	288, 487
BspLI	GGNNCC	5 cut(s)	70, 283, 445, 1272, 1294
BspMI	ACCTGC	1 cut(s)	1024
BspOI	GCTAGC	1 cut(s)	1019
BspPI	GGATC	3 cut(s)	398, 438, 451
BspT107I	GGYRCC	1 cut(s)	68
BsrDI	GCAATG	1 cut(s)	221
BsrI	ACTGG	2 cut(s)	397, 622
BssECI	CCNNGG	4 cut(s)	982, 1250, 1266, 1273
BssMI	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
BssSI	CACGAG	2 cut(s)	870, 1478
BssT1I	CCWWGG	1 cut(s)	1266
Bst2BI	CACGAG	2 cut(s)	870, 1478
Bst2UI	CCWGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
Bst4CI	ACNGT	4 cut(s)	305, 1246, 1445, 1503
BstAPI	GCANNNNNTGC	1 cut(s)	1226
BstC8I	GCNNGC	6 cut(s)	97, 127, 273, 703, 1017, 1106
BstDEI	CTNAG	6 cut(s)	11, 177, 360, 1076, 1347, 1458
BstDSI	CCRYGG	1 cut(s)	1266
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	7
BstKTI	GATC	7 cut(s)	406, 446, 511, 637, 885, 1126, 1386
BstMAI	GTCTC	2 cut(s)	581, 1167
BstMBI	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
BstMWI	GCNNNNNNNGC	8 cut(s)	13, 101, 389, 470, 770, 1016, 1188, 1226
BstNI	CCWGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
BstNSI	RCATGY	1 cut(s)	85
BstSCI	CCNGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
BstV1I	GCAGC	8 cut(s)	27, 91, 442, 781, 1241, 1373, 1378, 1381
BstV2I	GAAGAC	1 cut(s)	954
BstX2I	RGATCY	2 cut(s)	403, 443
BstYI	RGATCY	2 cut(s)	403, 443
BsuI	GTATCC	2 cut(s)	324, 734
BsuRI	GGCC	3 cut(s)	95, 667, 1376
BtgI	CCRYGG	1 cut(s)	1266
BtrI	CACGTC	2 cut(s)	255, 1258
BtsIMutI	CAGTG	4 cut(s)	310, 615, 787, 1025
BveI	ACCTGC	1 cut(s)	1024
Cac8I	GCNNGC	6 cut(s)	97, 127, 273, 703, 1017, 1106
CciI	TCATGA	2 cut(s)	288, 487
CfoI	GCGC	1 cut(s)	7
Cfr13I	GGNCC	4 cut(s)	282, 866, 989, 1270
Csp6I	GTAC	5 cut(s)	301, 709, 1051, 1404, 1492
CviAII	CATG	8 cut(s)	82, 289, 355, 488, 499, 536, 1267, 1288
CviQI	GTAC	5 cut(s)	301, 709, 1051, 1404, 1492
DdeI	CTNAG	6 cut(s)	11, 177, 360, 1076, 1347, 1458
DpnI	GATC	7 cut(s)	405, 445, 510, 636, 884, 1125, 1385
DpnII	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
DraIII	CACNNNGTG	1 cut(s)	863
EaeI	YGGCCR	1 cut(s)	93
EciI	GGCGGA	1 cut(s)	537
Ecl136II	GAGCTC	2 cut(s)	24, 199
Eco130I	CCWWGG	1 cut(s)	1266
Eco24I	GRGCYC	2 cut(s)	26, 201
Eco32I	GATATC	2 cut(s)	190, 564
Eco47I	GGWCC	4 cut(s)	282, 866, 989, 1270
Eco53kI	GAGCTC	2 cut(s)	24, 199
Eco57I	CTGAAG	2 cut(s)	874, 1049
Eco88I	CYCGRG	1 cut(s)	19
EcoICRI	GAGCTC	2 cut(s)	24, 199
EcoRII	CCWGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
EcoRV	GATATC	2 cut(s)	190, 564
EcoT14I	CCWWGG	1 cut(s)	1266
EcoT38I	GRGCYC	2 cut(s)	26, 201
ErhI	CCWWGG	1 cut(s)	1266
FaeI	CATG	8 cut(s)	85, 292, 358, 491, 502, 539, 1270, 1291
FalI	AAGNNNNNCTT	4 cut(s)	29, 61, 1361, 1393
FaqI	GGGAC	3 cut(s)	349, 1256, 1268
FatI	CATG	8 cut(s)	81, 288, 354, 487, 498, 535, 1266, 1287
FbaI	TGATCA	1 cut(s)	634
FriOI	GRGCYC	2 cut(s)	26, 201
FspBI	CTAG	5 cut(s)	324, 1016, 1109, 1194, 1311
GlaI	GCGC	1 cut(s)	6
GsaI	CCCAGC	1 cut(s)	1301
GsuI	CTGGAG	1 cut(s)	965
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	3 cut(s)	95, 667, 1376
HapII	CCGG	2 cut(s)	1046, 1134
HhaI	GCGC	1 cut(s)	7
Hin1II	CATG	8 cut(s)	85, 292, 358, 491, 502, 539, 1270, 1291
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HindIII	AAGCTT	3 cut(s)	462, 1056, 1469
HinfI	GANTC	7 cut(s)	173, 362, 495, 978, 1214, 1436, 1518
HpaII	CCGG	2 cut(s)	1046, 1134
HphI	GGTGA	3 cut(s)	277, 1174, 1457
Hpy166II	GTNNAC	1 cut(s)	866
Hpy188I	TCNGA	6 cut(s)	178, 244, 546, 639, 1441, 1459
Hpy8I	GTNNAC	1 cut(s)	866
HpyAV	CCTTC	6 cut(s)	214, 302, 403, 889, 1108, 1408
HpyCH4III	ACNGT	4 cut(s)	305, 1246, 1445, 1503
HpyCH4IV	ACGT	4 cut(s)	254, 379, 1257, 1307
HpyF10VI	GCNNNNNNNGC	8 cut(s)	13, 101, 389, 470, 770, 1016, 1188, 1226
HpyF3I	CTNAG	6 cut(s)	11, 177, 360, 1076, 1347, 1458
HpySE526I	ACGT	4 cut(s)	254, 379, 1257, 1307
Hsp92II	CATG	8 cut(s)	85, 292, 358, 491, 502, 539, 1270, 1291
HspAI	GCGC	1 cut(s)	5
Kpn2I	TCCGGA	1 cut(s)	1045
Ksp22I	TGATCA	1 cut(s)	634
Kzo9I	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
LmnI	GCTCC	1 cut(s)	29
Lsp1109I	GCAGC	8 cut(s)	27, 91, 442, 781, 1241, 1373, 1378, 1381
LweI	GCATC	2 cut(s)	258, 1348
MaeI	CTAG	5 cut(s)	324, 1016, 1109, 1194, 1311
MaeII	ACGT	4 cut(s)	254, 379, 1257, 1307
MaeIII	GTNAC	3 cut(s)	255, 835, 855
MalI	GATC	7 cut(s)	405, 445, 510, 636, 884, 1125, 1385
MboI	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
MboII	GAAGA	5 cut(s)	49, 877, 880, 959, 1393
MflI	RGATCY	2 cut(s)	403, 443
MhlI	GDGCHC	3 cut(s)	26, 201, 617
MlsI	TGGCCA	1 cut(s)	95
MluCI	AATT	5 cut(s)	247, 753, 1081, 1091, 1165
MluNI	TGGCCA	1 cut(s)	95
MlyI	GAGTC	2 cut(s)	371, 972
MmeI	TCCRAC	1 cut(s)	1532
MnlI	CCTC	9 cut(s)	97, 157, 329, 495, 525, 879, 1318, 1321, 1474
Mox20I	TGGCCA	1 cut(s)	95
MroI	TCCGGA	1 cut(s)	1045
MroXI	GAANNNNTTC	2 cut(s)	1071, 1418
MscI	TGGCCA	1 cut(s)	95
MseI	TTAA	2 cut(s)	141, 1169
MslI	CAYNNNNRTG	3 cut(s)	353, 780, 1286
Msp20I	TGGCCA	1 cut(s)	95
MspA1I	CMGCKG	1 cut(s)	1364
MspI	CCGG	2 cut(s)	1046, 1134
MspR9I	CCNGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
MvaI	CCWGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
MwoI	GCNNNNNNNGC	8 cut(s)	13, 101, 389, 470, 770, 1016, 1188, 1226
NcoI	CCATGG	1 cut(s)	1266
NdeII	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
NheI	GCTAGC	1 cut(s)	1015
NlaIII	CATG	8 cut(s)	85, 292, 358, 491, 502, 539, 1270, 1291
NlaIV	GGNNCC	5 cut(s)	70, 283, 445, 1272, 1294
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	2 cut(s)	255, 855
NspI	RCATGY	1 cut(s)	85
OliI	CACNNNNGTG	1 cut(s)	780
PaeR7I	CTCGAG	1 cut(s)	19
PagI	TCATGA	2 cut(s)	288, 487
PciI	ACATGT	1 cut(s)	81
PcsI	WCGNNNNNNNCGW	1 cut(s)	868
PdmI	GAANNNNTTC	2 cut(s)	1071, 1418
PfeI	GAWTC	5 cut(s)	173, 495, 1214, 1436, 1518
PflMI	CCANNNNNTGG	2 cut(s)	440, 1514
PfoI	TCCNGGA	1 cut(s)	973
PleI	GAGTC	2 cut(s)	370, 972
PpsI	GAGTC	2 cut(s)	370, 972
PscI	ACATGT	1 cut(s)	81
Psp124BI	GAGCTC	2 cut(s)	26, 201
Psp6I	CCWGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
PspFI	CCCAGC	1 cut(s)	1297
PspGI	CCWGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
PspN4I	GGNNCC	5 cut(s)	70, 283, 445, 1272, 1294
PspPI	GGNCC	4 cut(s)	282, 866, 989, 1270
PsuI	RGATCY	2 cut(s)	403, 443
PvuII	CAGCTG	1 cut(s)	1364
RsaI	GTAC	5 cut(s)	302, 710, 1052, 1405, 1493
RsaNI	GTAC	5 cut(s)	301, 709, 1051, 1404, 1492
RseI	CAYNNNNRTG	3 cut(s)	353, 780, 1286
SacI	GAGCTC	2 cut(s)	26, 201
SaqAI	TTAA	2 cut(s)	141, 1169
Sau3AI	GATC	7 cut(s)	403, 443, 508, 634, 882, 1123, 1383
Sau96I	GGNCC	4 cut(s)	282, 866, 989, 1270
SchI	GAGTC	2 cut(s)	371, 972
ScrFI	CCNGG	6 cut(s)	101, 318, 975, 983, 1251, 1275
SduI	GDGCHC	3 cut(s)	26, 201, 617
SfaNI	GCATC	2 cut(s)	258, 1348
Sfr274I	CTCGAG	1 cut(s)	19
SinI	GGWCC	4 cut(s)	282, 866, 989, 1270
SlaI	CTCGAG	1 cut(s)	19
SmiMI	CAYNNNNRTG	3 cut(s)	353, 780, 1286
SmlI	CTYRAG	4 cut(s)	19, 108, 161, 227
SmoI	CTYRAG	4 cut(s)	19, 108, 161, 227
Sse9I	AATT	5 cut(s)	247, 753, 1081, 1091, 1165
SsiI	CCGC	5 cut(s)	66, 158, 522, 727, 992
SspMI	CTAG	5 cut(s)	324, 1016, 1109, 1194, 1311
SstI	GAGCTC	2 cut(s)	26, 201
StyD4I	CCNGG	6 cut(s)	99, 316, 973, 981, 1249, 1273
StyI	CCWWGG	1 cut(s)	1266
TaaI	ACNGT	4 cut(s)	305, 1246, 1445, 1503
TaiI	ACGT	4 cut(s)	257, 382, 1260, 1310
TaqI	TCGA	7 cut(s)	20, 171, 195, 201, 351, 575, 1521
TasI	AATT	5 cut(s)	247, 753, 1081, 1091, 1165
TatI	WGTACW	2 cut(s)	300, 1050
TauI	GCSGC	2 cut(s)	69, 160
TfiI	GAWTC	5 cut(s)	173, 495, 1214, 1436, 1518
Tru1I	TTAA	2 cut(s)	141, 1169
Tru9I	TTAA	2 cut(s)	141, 1169
TscAI	CASTG	4 cut(s)	310, 622, 787, 1032
TseFI	GTSAC	2 cut(s)	255, 855
TseI	GCWGC	8 cut(s)	40, 104, 430, 794, 1229, 1361, 1391, 1394
Tsp45I	GTSAC	2 cut(s)	255, 855
TspDTI	ATGAA	3 cut(s)	357, 504, 960
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	4 cut(s)	310, 622, 787, 1032
Van91I	CCANNNNNTGG	2 cut(s)	440, 1514
VpaK11BI	GGWCC	4 cut(s)	282, 866, 989, 1270
XapI	RAATTY	3 cut(s)	247, 1091, 1165
XbaI	TCTAGA	1 cut(s)	1193
XceI	RCATGY	1 cut(s)	85
XhoI	CTCGAG	1 cut(s)	19
XmnI	GAANNNNTTC	2 cut(s)	1071, 1418
XspI	CTAG	5 cut(s)	324, 1016, 1109, 1194, 1311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.