Rh7CG456400

Interconversion of serine and glycine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001957
Physical Location & Seq
Forward (+)
59987306 .. 59993906
6601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG456400.1

Sequence Viewer

Length: 1560 bp
ATGGCAATGGCGCTTCGCAGGCTCTCAAGCGCCATCAACAGGCCACTGCTCAAACCTTTCGTCACTGCCAATGGCGGCTCCGTCTACCACATGTCCGCACTGTCCGGCCAAGCAGCTCAGGAAAAGGAGCAAGCTCGTACTACTTGGATAAAGCAACTGAATGAGCCGCTTGAGGTTATTGACCCCGAGATTGCTGATATCATCGAGCTTGAAAAAGCTCGGCAATGGAAGGGGCTTGAGCTTATTCCTTCAGAAAATTTCACATCACTGTCGGTGATGCAAGCTGTTGGTTCTGTCATGACAAACAAGTACAGCGAAGGATATCCCGGTGCTAGATACTATGGAGGGAATGAATACATCGACATGGCTGAGACCCTGTGTCAAAAACGCGCATTAGAAGCTTTTCAGTTAGATCCTGCAAAATGGGGAGTGAATGTCCAGTCATTATCTGGATCCCCTGCTAACTTCCAAGCGTACACTGGATTGTTGAAACCTCATGAAAGAATCATGGCTCTTGATCTGCCTCATGGTGGACATCTTTCTCATGGTTATCAGACTGACACCAAAAAGATATCTGCTGTCTCGATATTCTTCGAGACAATGCCATACAGATTGGACGAAACAACTGGTTACATTGACTATGATCAGTTGGAGAAAAGTGCCACACTATTCAGACCCAAATTAATAGTAGCTGGTGCCAGTGCTTATGCACGCTTGTATGATTATGCACGTATACGCAAGGTCTGTGATAAGCAGAAAGCTATTTTGTTAGCTGATATGGCACACATCAGTGGATTGGTTGCGGCAGGTGTTATTCCATCTCCTTTTGAGTATGCAGATATTGTTACAACCACAACTCACAAGTCACTTCGTGGCCCTCGTGGGGCTATGATCTTCTTCAGGAAGGGGGTCAAAAATGTAAACAAGCAAGGACAAGAAGTTATGTATGACTATGAAGACAAAATTAATCAGGCTGTCTTTCCTGGACTCCAAGGTGGTCCACACAATCACACCATATCTGGGTTAGCAGTTGCACTCAAGCAGGCCACAACTCCAGAGTTCAAAGCTTACCAAGAGCAAGTTCTCAGTAACTCCGCAAAATTTGCAGAGAGCTTGCTAGAGAAGGGCTATGATCTTGTCTCTGGTGGAACTGACAACCATTTGGTGTTGGTAAATTTGAGGGACAAGGGAATTGATGGCTCAAGAGTTGAAAAGGTTCTGGAAGCTGCTCATATTGCAGCAAATAAAAATACTGTCCCAGGGGATGTTTCTGCCATGGTCCCTGGTGGTATACGTATGGGAACCCCGGCTCTCACATCTAGGGGTTTTATGGAGGATGATTTTGAAAAAGTATCTGAGTATTTTGATGCAGCCGTGAAGATAGCCTTGAAGATTAAGGCTGCTACAAAAGGTACCAAGTTAAAGGATTTTGTGGCAACTCTGGAATCAGATGGTCAAATACAATCTGAGATTGCAAAGCTACGCCACGAGGTTGAGCAGTATGCTAAAGAATTTCCAACTATTGGATTTGAGAAAGAAACAATGAGATACAAAGAATGA

Protein Analysis

519

Amino Acids

57.28

Weight (kDa)

6.89

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 58 - 454 4e-213 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 797
Acc65I GGTACC 1 cut(s) 1412
AccB1I GGYRCC 2 cut(s) 695, 1412
AccB7I CCANNNNNTGG 1 cut(s) 1523
AccI GTMKAC 3 cut(s) 84, 733, 1291
AccII CGCG 1 cut(s) 390
AciI CCGC 5 cut(s) 75, 96, 167, 803, 1095
AclWI GGATC 3 cut(s) 407, 447, 460
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 4 cut(s) 256, 1100, 1174, 1511
AcuI CTGAAG 2 cut(s) 234, 883
AdeI CACNNNGTG 1 cut(s) 872
AfaI GTAC 4 cut(s) 139, 311, 476, 1414
AfiI CCNNNNNNNGG 5 cut(s) 39, 530, 883, 1020, 1523
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 6 cut(s) 212, 490, 1063, 1211, 1346, 1390
AhdI GACNNNNNGTC 1 cut(s) 378
AjnI CCWGG 3 cut(s) 982, 1258, 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 1065, 1097
AleI CACNNNNGTG 1 cut(s) 789
Alw26I GTCTC 4 cut(s) 365, 586, 590, 1144
AlwI GGATC 3 cut(s) 407, 447, 460
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 4 cut(s) 41, 106, 874, 1044
ApeKI GCWGC 5 cut(s) 113, 1226, 1238, 1370, 1400
ApoI RAATTY 4 cut(s) 256, 1100, 1174, 1511
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AseI ATTAAT 2 cut(s) 683, 966
Asp700I GAANNNNTTC 2 cut(s) 402, 1215
Asp718I GGTACC 1 cut(s) 1412
AspLEI GCGC 3 cut(s) 13, 32, 392
AspS9I GGNCC 3 cut(s) 875, 998, 1279
AsuC2I CCSGG 2 cut(s) 327, 1307
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 2 cut(s) 998, 1279
BamHI GGATCC 1 cut(s) 452
BanI GGYRCC 2 cut(s) 695, 1412
BauI CACGAG 2 cut(s) 879, 1487
BbsI GAAGAC 1 cut(s) 963
BbvI GCAGC 5 cut(s) 125, 1213, 1250, 1382, 1387
BccI CCATC 4 cut(s) 41, 826, 1190, 1445
BceAI ACGGC 1 cut(s) 1358
BciT130I CCWGG 3 cut(s) 984, 1260, 1284
BclI TGATCA 1 cut(s) 643
BcnI CCSGG 2 cut(s) 327, 1307
BcoDI GTCTC 4 cut(s) 365, 586, 590, 1144
BfaI CTAG 3 cut(s) 333, 1118, 1320
BfoI RGCGCY 2 cut(s) 14, 33
BfuAI ACCTGC 1 cut(s) 797
BisI GCNGC 8 cut(s) 76, 114, 167, 804, 1227, 1239, 1371, 1401
BlsI GCNGC 8 cut(s) 77, 115, 168, 805, 1228, 1240, 1372, 1402
Bme1390I CCNGG 5 cut(s) 327, 984, 1260, 1284, 1307
Bme18I GGWCC 2 cut(s) 998, 1279
BmeRI GACNNNNNGTC 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 3 cut(s) 875, 998, 1279
BmiI GGNNCC 6 cut(s) 79, 454, 697, 1281, 1303, 1414
BmrFI CCNGG 5 cut(s) 327, 984, 1260, 1284, 1307
BmsI GCATC 2 cut(s) 267, 1357
BpiI GAAGAC 1 cut(s) 963
BpmI CTGGAG 1 cut(s) 1038
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 5 cut(s) 10, 191, 257, 1022, 1186
BpuMI CCSGG 2 cut(s) 327, 1307
BsaAI YACGTR 2 cut(s) 731, 1295
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 6 cut(s) 991, 1258, 1259, 1275, 1282, 1305
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 530, 883, 1020, 1523
Bse1I ACTGG 4 cut(s) 439, 484, 631, 699
Bse3DI GCAATG 2 cut(s) 12, 230
BseBI CCWGG 3 cut(s) 984, 1260, 1284
BseDI CCNNGG 6 cut(s) 991, 1258, 1259, 1275, 1282, 1305
BseGI GGATG 2 cut(s) 1270, 1342
BseLI CCNNNNNNNGG 5 cut(s) 39, 530, 883, 1020, 1523
BseMI GCAATG 2 cut(s) 12, 230
BseMII CTCAG 5 cut(s) 131, 360, 1099, 1347, 1458
BseNI ACTGG 4 cut(s) 439, 484, 631, 699
BseXI GCAGC 5 cut(s) 125, 1213, 1250, 1382, 1387
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 43, 108, 876, 1046
BshNI GGYRCC 2 cut(s) 695, 1412
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 3 cut(s) 105, 327, 1307
BslFI GGGAC 3 cut(s) 1196, 1241, 1265
BslI CCNNNNNNNGG 5 cut(s) 39, 530, 883, 1020, 1523
BsmAI GTCTC 4 cut(s) 365, 586, 590, 1144
BsmFI GGGAC 3 cut(s) 1196, 1241, 1265
BsnI GGCC 4 cut(s) 43, 108, 876, 1046
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 185
Bsp143I GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
Bsp19I CCATGG 1 cut(s) 1275
BspACI CCGC 5 cut(s) 75, 96, 167, 803, 1095
BspANI GGCC 4 cut(s) 43, 108, 876, 1046
BspCNI CTCAG 5 cut(s) 130, 361, 1098, 1348, 1459
BspFNI CGCG 1 cut(s) 390
BspHI TCATGA 2 cut(s) 297, 496
BspLI GGNNCC 6 cut(s) 79, 454, 697, 1281, 1303, 1414
BspMI ACCTGC 1 cut(s) 797
BspPI GGATC 3 cut(s) 407, 447, 460
BspT107I GGYRCC 2 cut(s) 695, 1412
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 12, 230
BsrI ACTGG 4 cut(s) 439, 484, 631, 699
BssECI CCNNGG 6 cut(s) 991, 1258, 1259, 1275, 1282, 1305
BssMI GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
BssNAI GTATAC 2 cut(s) 734, 1292
BssSI CACGAG 2 cut(s) 879, 1487
BssT1I CCWWGG 2 cut(s) 991, 1275
Bst1107I GTATAC 2 cut(s) 734, 1292
Bst2BI CACGAG 2 cut(s) 879, 1487
Bst2UI CCWGG 3 cut(s) 984, 1260, 1284
Bst4CI ACNGT 3 cut(s) 102, 270, 1255
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstBAI YACGTR 2 cut(s) 731, 1295
BstC8I GCNNGC 6 cut(s) 20, 132, 282, 712, 1044, 1115
BstDEI CTNAG 5 cut(s) 117, 369, 1085, 1356, 1467
BstDSI CCRYGG 1 cut(s) 1275
BstF5I GGATG 2 cut(s) 1270, 1342
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 2 cut(s) 14, 33
BstHHI GCGC 3 cut(s) 13, 32, 392
BstKTI GATC 6 cut(s) 415, 455, 520, 646, 894, 1135
BstMAI GTCTC 4 cut(s) 365, 586, 590, 1144
BstMBI GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
BstMWI GCNNNNNNNGC 5 cut(s) 19, 398, 779, 1103, 1235
BstNI CCWGG 3 cut(s) 984, 1260, 1284
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 5 cut(s) 325, 982, 1258, 1282, 1305
BstSNI TACGTA 1 cut(s) 1295
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 5 cut(s) 125, 1213, 1250, 1382, 1387
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 2 cut(s) 412, 452
BstYI RGATCY 2 cut(s) 412, 452
BstZ17I GTATAC 2 cut(s) 734, 1292
BsuRI GGCC 4 cut(s) 43, 108, 876, 1046
BtgI CCRYGG 1 cut(s) 1275
BtsCI GGATG 2 cut(s) 1270, 1342
BtsI GCAGTG 2 cut(s) 44, 63
BtsIMutI CAGTG 7 cut(s) 44, 63, 98, 266, 477, 706, 796
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 6 cut(s) 20, 132, 282, 712, 1044, 1115
CciI TCATGA 2 cut(s) 297, 496
CfoI GCGC 3 cut(s) 13, 32, 392
Cfr13I GGNCC 3 cut(s) 875, 998, 1279
Csp6I GTAC 4 cut(s) 138, 310, 475, 1413
CviAII CATG 8 cut(s) 91, 298, 364, 497, 508, 527, 545, 1276
CviQI GTAC 4 cut(s) 138, 310, 475, 1413
DdeI CTNAG 5 cut(s) 117, 369, 1085, 1356, 1467
DpnI GATC 6 cut(s) 414, 454, 519, 645, 893, 1134
DpnII GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
DraIII CACNNNGTG 1 cut(s) 872
DriI GACNNNNNGTC 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 106
Eam1105I GACNNNNNGTC 1 cut(s) 378
Eco105I TACGTA 1 cut(s) 1295
Eco130I CCWWGG 2 cut(s) 991, 1275
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 3 cut(s) 199, 323, 573
Eco47I GGWCC 2 cut(s) 998, 1279
Eco57I CTGAAG 2 cut(s) 234, 883
Eco88I CYCGRG 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 982, 1258, 1282
EcoRV GATATC 3 cut(s) 199, 323, 573
EcoT14I CCWWGG 2 cut(s) 991, 1275
ErhI CCWWGG 2 cut(s) 991, 1275
FaeI CATG 8 cut(s) 94, 301, 367, 500, 511, 530, 548, 1279
FalI AAGNNNNNCTT 2 cut(s) 1370, 1402
FaqI GGGAC 3 cut(s) 1196, 1241, 1265
FatI CATG 8 cut(s) 90, 297, 363, 496, 507, 526, 544, 1275
FbaI TGATCA 1 cut(s) 643
FblI GTMKAC 3 cut(s) 84, 733, 1291
Fnu4HI GCNGC 8 cut(s) 76, 114, 167, 804, 1227, 1239, 1371, 1401
FokI GGATG 2 cut(s) 1277, 1349
Fsp4HI GCNGC 8 cut(s) 76, 114, 167, 804, 1227, 1239, 1371, 1401
FspBI CTAG 3 cut(s) 333, 1118, 1320
GlaI GCGC 3 cut(s) 12, 31, 391
GluI GCNGC 8 cut(s) 76, 114, 167, 804, 1227, 1239, 1371, 1401
GsuI CTGGAG 1 cut(s) 1038
HaeII RGCGCY 2 cut(s) 14, 33
HaeIII GGCC 4 cut(s) 43, 108, 876, 1046
HapII CCGG 3 cut(s) 105, 327, 1307
HhaI GCGC 3 cut(s) 13, 32, 392
Hin1II CATG 8 cut(s) 94, 301, 367, 500, 511, 530, 548, 1279
Hin6I GCGC 3 cut(s) 11, 30, 390
HinP1I GCGC 3 cut(s) 11, 30, 390
HindIII AAGCTT 2 cut(s) 399, 1065
HinfI GANTC 3 cut(s) 504, 987, 1445
HpaII CCGG 3 cut(s) 105, 327, 1307
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 7 cut(s) 85, 477, 533, 734, 922, 1001, 1292
Hpy188I TCNGA 6 cut(s) 253, 555, 674, 1357, 1450, 1468
Hpy8I GTNNAC 7 cut(s) 85, 477, 533, 734, 922, 1001, 1292
HpyAV CCTTC 5 cut(s) 223, 258, 311, 898, 1117
HpyCH4III ACNGT 3 cut(s) 102, 270, 1255
HpyCH4IV ACGT 2 cut(s) 730, 1294
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 398, 779, 1103, 1235
HpyF3I CTNAG 5 cut(s) 117, 369, 1085, 1356, 1467
HpySE526I ACGT 2 cut(s) 730, 1294
Hsp92II CATG 8 cut(s) 94, 301, 367, 500, 511, 530, 548, 1279
HspAI GCGC 3 cut(s) 11, 30, 390
KpnI GGTACC 1 cut(s) 1416
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
LmnI GCTCC 2 cut(s) 83, 127
Lsp1109I GCAGC 5 cut(s) 125, 1213, 1250, 1382, 1387
LweI GCATC 2 cut(s) 267, 1357
MaeI CTAG 3 cut(s) 333, 1118, 1320
MaeII ACGT 2 cut(s) 730, 1294
MaeIII GTNAC 5 cut(s) 61, 629, 844, 864, 1088
MalI GATC 6 cut(s) 414, 454, 519, 645, 893, 1134
MboI GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
MboII GAAGA 6 cut(s) 583, 886, 889, 968, 1390, 1402
MflI RGATCY 2 cut(s) 412, 452
MluCI AATT 7 cut(s) 256, 680, 963, 1100, 1174, 1191, 1511
MlyI GAGTC 1 cut(s) 981
MmeI TCCRAC 2 cut(s) 630, 1541
MnlI CCTC 8 cut(s) 166, 338, 504, 534, 888, 1173, 1327, 1483
MroXI GAANNNNTTC 2 cut(s) 402, 1215
MseI TTAA 4 cut(s) 683, 966, 1395, 1421
MslI CAYNNNNRTG 2 cut(s) 362, 789
MspI CCGG 3 cut(s) 105, 327, 1307
MspR9I CCNGG 5 cut(s) 327, 984, 1260, 1284, 1307
MvaI CCWGG 3 cut(s) 984, 1260, 1284
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 5 cut(s) 19, 398, 779, 1103, 1235
NciI CCSGG 2 cut(s) 327, 1307
NcoI CCATGG 1 cut(s) 1275
NdeII GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
NlaIII CATG 8 cut(s) 94, 301, 367, 500, 511, 530, 548, 1279
NlaIV GGNNCC 6 cut(s) 79, 454, 697, 1281, 1303, 1414
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 61, 864
NspI RCATGY 1 cut(s) 94
OliI CACNNNNGTG 1 cut(s) 789
PagI TCATGA 2 cut(s) 297, 496
PaqCI CACCTGC 1 cut(s) 797
PasI CCCWGGG 1 cut(s) 1259
PciI ACATGT 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 877
PdmI GAANNNNTTC 2 cut(s) 402, 1215
PfeI GAWTC 2 cut(s) 504, 1445
PflMI CCANNNNNTGG 1 cut(s) 1523
PfoI TCCNGGA 1 cut(s) 982
PkrI GCNGC 8 cut(s) 77, 115, 168, 805, 1228, 1240, 1372, 1402
PleI GAGTC 1 cut(s) 981
PpsI GAGTC 1 cut(s) 981
Ppu21I YACGTR 2 cut(s) 731, 1295
PscI ACATGT 1 cut(s) 90
PshBI ATTAAT 2 cut(s) 683, 966
Psp6I CCWGG 3 cut(s) 982, 1258, 1282
PspGI CCWGG 3 cut(s) 982, 1258, 1282
PspN4I GGNNCC 6 cut(s) 79, 454, 697, 1281, 1303, 1414
PspPI GGNCC 3 cut(s) 875, 998, 1279
PsuI RGATCY 2 cut(s) 412, 452
RsaI GTAC 4 cut(s) 139, 311, 476, 1414
RsaNI GTAC 4 cut(s) 138, 310, 475, 1413
RseI CAYNNNNRTG 2 cut(s) 362, 789
SaqAI TTAA 4 cut(s) 683, 966, 1395, 1421
SatI GCNGC 8 cut(s) 76, 114, 167, 804, 1227, 1239, 1371, 1401
Sau3AI GATC 6 cut(s) 412, 452, 517, 643, 891, 1132
Sau96I GGNCC 3 cut(s) 875, 998, 1279
SchI GAGTC 1 cut(s) 981
ScrFI CCNGG 5 cut(s) 327, 984, 1260, 1284, 1307
SfaNI GCATC 2 cut(s) 267, 1357
SinI GGWCC 2 cut(s) 998, 1279
SmiMI CAYNNNNRTG 2 cut(s) 362, 789
SmlI CTYRAG 5 cut(s) 25, 170, 236, 1037, 1201
SmoI CTYRAG 5 cut(s) 25, 170, 236, 1037, 1201
SnaBI TACGTA 1 cut(s) 1295
Sse9I AATT 7 cut(s) 256, 680, 963, 1100, 1174, 1191, 1511
SsiI CCGC 5 cut(s) 75, 96, 167, 803, 1095
SspMI CTAG 3 cut(s) 333, 1118, 1320
StyD4I CCNGG 5 cut(s) 325, 982, 1258, 1282, 1305
StyI CCWWGG 2 cut(s) 991, 1275
TaaI ACNGT 3 cut(s) 102, 270, 1255
TaiI ACGT 2 cut(s) 733, 1297
TaqI TCGA 4 cut(s) 204, 360, 584, 594
TasI AATT 7 cut(s) 256, 680, 963, 1100, 1174, 1191, 1511
TatI WGTACW 1 cut(s) 309
TauI GCSGC 3 cut(s) 78, 169, 806
TfiI GAWTC 2 cut(s) 504, 1445
Tru1I TTAA 4 cut(s) 683, 966, 1395, 1421
Tru9I TTAA 4 cut(s) 683, 966, 1395, 1421
TscAI CASTG 7 cut(s) 51, 70, 105, 273, 484, 706, 796
TseFI GTSAC 2 cut(s) 61, 864
TseI GCWGC 5 cut(s) 113, 1226, 1238, 1370, 1400
Tsp45I GTSAC 2 cut(s) 61, 864
TspDTI ATGAA 3 cut(s) 366, 513, 969
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 7 cut(s) 51, 70, 105, 273, 484, 706, 796
Van91I CCANNNNNTGG 1 cut(s) 1523
VpaK11BI GGWCC 2 cut(s) 998, 1279
VspI ATTAAT 2 cut(s) 683, 966
XapI RAATTY 4 cut(s) 256, 1100, 1174, 1511
XceI RCATGY 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 2 cut(s) 446, 476
XmiI GTMKAC 3 cut(s) 84, 733, 1291
XmnI GAANNNNTTC 2 cut(s) 402, 1215
XspI CTAG 3 cut(s) 333, 1118, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.