Rh4DG362000

Interconversion of serine and glycine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001957
Physical Location & Seq
Reverse (-)
58390414 .. 58393806
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG362000.1

Sequence Viewer

Length: 519 bp
ATGATCTCTAACGAGAGTTACCAGACAGGCATCTCGAACTGTCGCAAGCGACCTTCGCCGCCTTTCGCTGCCGCAAGGACCGTGTGCTCCACCTCAATCTCCACCTCTTGGAGGACTGAATCGCCACCACCGTGGACGGTTATGATGGTATTCTGCATGCTTCTCCGGAGAAAGTACAGCGCCCTGCTCTATTCTTTGAGGGATATTGAAGATAGTGCCACACTATTCAGACCCAAATTAATAGTTGCTGGTGCCAGTGCTTGTGCACGCTTGTACGACTATGCATGCATACGCAAGGTCTGTGATAAGCAGAAAGCTATTTTGTTAGCTGATATGGCACACATCAGTGGATTGGTTGCGGCATGTGTTATTCCATCTCCTTTTGAGTATGCAGATATTGTTACAACCACAACTCATAAGTCATTTCGTGGGGCTATGATCTTTTTCAGGAAGGGGGTCATAGCCCCACGAGGCCACAACTCCAGAGTTCAAAGCTTACCAAGAGCAAGTTCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004372 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0005886 GO:0006082 GO:0006520 GO:0006544 GO:0006563 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008150 GO:0008152 GO:0008187 GO:0008219 GO:0008266 GO:0009069 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009626 GO:0009628 GO:0009853 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0012501 GO:0016020 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019752 GO:0022626 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033554 GO:0034050 GO:0042133 GO:0042221 GO:0043094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0046686 GO:0048046 GO:0048511 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901605 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

172

Amino Acids

19.07

Weight (kDa)

9.85

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 66 - 154 7.3e-38 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 108
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 3 cut(s) 59, 72, 359
AfaI GTAC 2 cut(s) 176, 275
AfiI CCNNNNNNNGG 2 cut(s) 108, 111
AgsI TTSAA 2 cut(s) 209, 491
AjuI GAANNNNNNNTTGG 1 cut(s) 493
AleI CACNNNNGTG 2 cut(s) 130, 345
AluBI AGCT 3 cut(s) 317, 329, 495
AluI AGCT 3 cut(s) 317, 329, 495
Alw21I GWGCWC 2 cut(s) 89, 268
Alw44I GTGCAC 1 cut(s) 264
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 1 cut(s) 472
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 1 cut(s) 68
AseI ATTAAT 1 cut(s) 239
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 78
AvaII GGWCC 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 251
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 89, 268
BbvI GCAGC 1 cut(s) 55
BccI CCATC 2 cut(s) 139, 382
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 4 cut(s) 59, 69, 72, 360
BlsI GCNGC 4 cut(s) 60, 70, 73, 361
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 1 cut(s) 253
BmsI GCATC 1 cut(s) 39
BpmI CTGGAG 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 131
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 111
Bse1I ACTGG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 131
BseLI CCNNNNNNNGG 2 cut(s) 108, 111
BseNI ACTGG 1 cut(s) 255
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 1 cut(s) 55
BshFI GGCC 1 cut(s) 474
BshNI GGYRCC 1 cut(s) 251
BsiHKAI GWGCWC 2 cut(s) 89, 268
BsiSI CCGG 1 cut(s) 166
BslI CCNNNNNNNGG 2 cut(s) 108, 111
BsnI GGCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 89, 268
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 2 cut(s) 3, 438
BspACI CCGC 3 cut(s) 59, 72, 359
BspANI GGCC 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 2 cut(s) 3, 438
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 41, 82, 132, 139
BstC8I GCNNGC 4 cut(s) 47, 158, 268, 286
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 131
BstENI CCTNNNNNAGG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 6, 441
BstMBI GATC 2 cut(s) 3, 438
BstMWI GCNNNNNNNGC 2 cut(s) 55, 335
BstNSI RCATGY 3 cut(s) 160, 288, 366
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 1 cut(s) 55
BstXI CCANNNNNNTGG 1 cut(s) 132
BsuRI GGCC 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 262, 352
Cac8I GCNNGC 4 cut(s) 47, 158, 268, 286
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 78
Csp6I GTAC 2 cut(s) 175, 274
CviAII CATG 3 cut(s) 157, 285, 363
CviJI RGCY 6 cut(s) 317, 329, 434, 464, 474, 495
CviKI_1 RGCY 6 cut(s) 317, 329, 434, 464, 474, 495
CviQI GTAC 2 cut(s) 175, 274
DdeI CTNAG 1 cut(s) 513
DpnI GATC 2 cut(s) 5, 440
DpnII GATC 2 cut(s) 3, 438
Eco47I GGWCC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoT22I ATGCAT 2 cut(s) 286, 290
FaeI CATG 3 cut(s) 160, 288, 366
FatI CATG 3 cut(s) 156, 284, 362
Fnu4HI GCNGC 4 cut(s) 59, 69, 72, 360
Fsp4HI GCNGC 4 cut(s) 59, 69, 72, 360
GlaI GCGC 1 cut(s) 181
GluI GCNGC 4 cut(s) 59, 69, 72, 360
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 166
HhaI GCGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 160, 288, 366
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 135, 266
Hpy188I TCNGA 1 cut(s) 230
Hpy188III TCNNGA 4 cut(s) 34, 166, 448, 483
Hpy8I GTNNAC 2 cut(s) 135, 266
HpyAV CCTTC 2 cut(s) 63, 445
HpyCH4III ACNGT 4 cut(s) 41, 82, 132, 139
HpyCH4V TGCA 5 cut(s) 156, 266, 284, 288, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 335
HpyF3I CTNAG 1 cut(s) 513
Hsp92II CATG 3 cut(s) 160, 288, 366
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 3, 438
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 8 cut(s) 12, 35, 179, 197, 234, 268, 433, 496
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 17, 400
MalI GATC 2 cut(s) 5, 440
MboI GATC 2 cut(s) 3, 438
MboII GAAGA 1 cut(s) 221
MhlI GDGCHC 2 cut(s) 89, 268
MluCI AATT 1 cut(s) 236
MnlI CCTC 5 cut(s) 103, 105, 115, 192, 464
Mph1103I ATGCAT 2 cut(s) 286, 290
MroI TCCGGA 1 cut(s) 165
MseI TTAA 1 cut(s) 239
MslI CAYNNNNRTG 2 cut(s) 130, 345
MspI CCGG 1 cut(s) 166
MwoI GCNNNNNNNGC 2 cut(s) 55, 335
NdeII GATC 2 cut(s) 3, 438
NlaIII CATG 3 cut(s) 160, 288, 366
NlaIV GGNNCC 1 cut(s) 253
NsiI ATGCAT 2 cut(s) 286, 290
NspI RCATGY 3 cut(s) 160, 288, 366
OliI CACNNNNGTG 2 cut(s) 130, 345
PaeI GCATGC 2 cut(s) 160, 288
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PfeI GAWTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 4 cut(s) 60, 70, 73, 361
PshBI ATTAAT 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 78
RsaI GTAC 2 cut(s) 176, 275
RsaNI GTAC 2 cut(s) 175, 274
RseI CAYNNNNRTG 2 cut(s) 130, 345
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 4 cut(s) 59, 69, 72, 360
Sau3AI GATC 2 cut(s) 3, 438
Sau96I GGNCC 1 cut(s) 78
SduI GDGCHC 2 cut(s) 89, 268
SetI ASST 7 cut(s) 55, 95, 107, 300, 319, 331, 497
SfaNI GCATC 1 cut(s) 39
SinI GGWCC 1 cut(s) 78
SmiMI CAYNNNNRTG 2 cut(s) 130, 345
SphI GCATGC 2 cut(s) 160, 288
Sse9I AATT 1 cut(s) 236
SsiI CCGC 3 cut(s) 59, 72, 359
TaaI ACNGT 4 cut(s) 41, 82, 132, 139
TaqI TCGA 1 cut(s) 35
TasI AATT 1 cut(s) 236
TatI WGTACW 1 cut(s) 174
TauI GCSGC 3 cut(s) 61, 74, 362
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 2 cut(s) 262, 352
TseI GCWGC 1 cut(s) 68
TspRI CASTG 2 cut(s) 262, 352
Van91I CCANNNNNTGG 1 cut(s) 108
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 78
VspI ATTAAT 1 cut(s) 239
XagI CCTNNNNNAGG 1 cut(s) 109
XceI RCATGY 3 cut(s) 160, 288, 366
Zsp2I ATGCAT 2 cut(s) 286, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.