Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001957

Physical Location & Seq

Genomic Coordinates Reverse (-)

11416757 .. 11423187

Gene Structure 6431 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00227300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCAATGGCGCTTCGCAGGCTCTCAAGCGCCATCAACAGGCCACTGCTCAAACCTTTCGTCACTGCCAATGGCGGCTCCGTCTACCACATGTCCGCACTGTCCAGCCAAGCAGCTCAGGAAAAGGACCAAGCTCGTACTACTTGGATAAAGCAACTGAATGAGCCGCTTGAGGTTATTGATCCCGAGATTGCTGATATCATCGAGCTTGAAAAAGCTCGGCAATGGAAGGGGCTTGAGCTTATTCCTTCAGAAAATTTCACATCACTGTCGGTGATGCAAGCTGTTGGTTCTGTCATGACCAACAAGTACAGTGAAGGATATCCCGGTGCTAGATACTATGGAGGAAATGAATACATTGACATGGCTGAGACCTTGTGTCAAAAACGCGCATTAGAAGCTTTTCAGTTAGATCCTGCAAAATGGGGAGTGAATGTCCAGTCATTATCCGGATCCCCTGCTAACTTCCAAGTGTACACTGGATTGTTGAAACCTCATGAAAGAATCATGGCTCTTGATCTGCCTCATGGTGGACATCTTTCTCATGGTTATCAGACTGACACCAAAAAGATATCTGCTGTCTCGATATTCTTCGAGACAATGCCATACAGATTGGATGAAACCACTGGTTACATTGACTATGATCAGTTGGAGAAAAGTGCCACACTATTCAGACCCAAATTAATAGTAGCTGGTGCCAGTGCTTATGCACGCTTGTATGATTATGCACGTATACGCAAGGTCTGTGATAAGCAGAAAGCTATTTTGTTAGCTGATATGGCACACATCAGTGGATTGGTTGCGGCAGGTGTTATTCCATCTCCTTTTGAGTATGCAGATATTGTTACAACCACAACTCACAAGTCACTTCGTGGCCCTCGTGGGGCTATGATCTTCTTCAGGAAGGGGGTAAAAAATGTAAACAAGCAAGGACAAGAAGTTATGTATGACTATGAAGACAAAATTAATCAGGCTGTCTTTCCTGGACTCCAAGGTGGTCCACACAATCACACCATATCTGGGTTAGCAGTTGCACTCAAGCAGGCCACAACTCCAGAGTTCAAAGCTTACCAAGAGCAAGTTCTCAGTAACTCCGCAAAATTTGCAGAGAGCTTGCTAGAGAAGGGCTATGATCTTGTCTCTGGTGGAACTGACAACCATTTAGTGTTGGTAAATTTGAGGGACAAGGGAATTGATGGCTCAAGAGTTGAAAAGGTTCTGGAAGCTGCTCATATTGCAGCAAATAAAAATACTGTCCCAGGGGATGTTTCTGCCATGGTCCCTGGTGGTATACGTATGGGAACCCCGGCTCTCACATCTAGGGGTTTTATGGAGGATGACTTTGAAAAAGTAGCAGAGTATTTTGATGCAGCCGTGAAGATAACCTTGAAGATTAAGGCTGCTACAAAAGGTACCAAGTTAAAGGATTTTGTGGCAACTCTGGAATCAGATGGTCAAATACAATCTGAGATTGCAAAGCTACGACATGAGGTTGAGCAGTATGCTAAAGAATTTCCAACTATTGGATTTGAGAAAGAAACAATGAGATACAAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

519

Amino Acids

57.34

Weight (kDa)

6.89

Isoelectric Point (pI)

32.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	58 - 454	2.7e-214	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26780 AT5G26780 AT5G26780 AT5G26780
fragaria_vesca	FvH4_5g34000 FvH4_5g34000 FvH4_5g34000
malus_domestica	MD08G1206800.v1.1 MD15G1390500.v1.1
prunus_persica	Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1 Prupe.1G539400_v2.0.a1
pyrus_communis	pycom08g17860
rosa_chinensis	RchiOBHm_Chr5g0052761 RchiOBHm_Chr7g0233441 RchiOBHm_Chr7g0235741
rosa_laevigata	RLG00000001270 RLG00000013832 RLG00000035486
rosa_multiflora	Rmu_sc0000029.1_g000053 Rmu_sc0006510.1_g000006
rosa_roxburghii	Rroxscaffold_3G00227300 Rroxscaffold_3G00241170
rosa_rugosa	Rorug07G0280700
rosa_samantha	Rh1CG020600 Rh1CG022300 Rh2AG156000 Rh2BG162300 Rh2BG221600 Rh2DG161400 Rh4DG362000 Rh5BG352800 Rh5CG247000 Rh5CG419500 Rh5DG369100 Rh7AG154700 Rh7AG436500 Rh7AG488000 Rh7BG409600 Rh7CG456400 Rh7DG425700
rosa_wichuraiana	Rw0G022490 Rw7G035990 Rw7G036020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	797
Acc36I	ACCTGC	1 cut(s)	797
Acc65I	GGTACC	1 cut(s)	1412
AccB1I	GGYRCC	2 cut(s)	695, 1412
AccB7I	CCANNNNNTGG	1 cut(s)	1523
AccI	GTMKAC	3 cut(s)	84, 733, 1291
AccII	CGCG	1 cut(s)	390
AccIII	TCCGGA	1 cut(s)	449
AciI	CCGC	5 cut(s)	75, 96, 167, 803, 1095
AclWI	GGATC	4 cut(s)	176, 407, 447, 460
AcsI	RAATTY	4 cut(s)	256, 1100, 1174, 1511
AcuI	CTGAAG	2 cut(s)	234, 883
AdeI	CACNNNGTG	1 cut(s)	872
AfaI	GTAC	4 cut(s)	139, 311, 476, 1414
AfiI	CCNNNNNNNGG	5 cut(s)	39, 530, 883, 1020, 1523
AflIII	ACRYGT	1 cut(s)	90
AgsI	TTSAA	6 cut(s)	212, 490, 1063, 1211, 1346, 1390
AhdI	GACNNNNNGTC	1 cut(s)	378
AjnI	CCWGG	3 cut(s)	982, 1258, 1282
AjuI	GAANNNNNNNTTGG	2 cut(s)	1065, 1097
AleI	CACNNNNGTG	1 cut(s)	789
Alw26I	GTCTC	4 cut(s)	365, 586, 590, 1144
AlwI	GGATC	4 cut(s)	176, 407, 447, 460
Ama87I	CYCGRG	1 cut(s)	185
Aor13HI	TCCGGA	1 cut(s)	449
AoxI	GGCC	3 cut(s)	41, 874, 1044
ApeKI	GCWGC	5 cut(s)	113, 1226, 1238, 1370, 1400
ApoI	RAATTY	4 cut(s)	256, 1100, 1174, 1511
ArsI	GACNNNNNNTTYG	2 cut(s)	45, 77
AseI	ATTAAT	2 cut(s)	683, 966
Asp700I	GAANNNNTTC	2 cut(s)	402, 1215
Asp718I	GGTACC	1 cut(s)	1412
AspLEI	GCGC	3 cut(s)	13, 32, 392
AspS9I	GGNCC	4 cut(s)	127, 875, 998, 1279
AsuC2I	CCSGG	2 cut(s)	327, 1307
AsuHPI	GGTGA	1 cut(s)	286
AvaI	CYCGRG	1 cut(s)	185
AvaII	GGWCC	3 cut(s)	127, 998, 1279
BamHI	GGATCC	1 cut(s)	452
BanI	GGYRCC	2 cut(s)	695, 1412
BauI	CACGAG	1 cut(s)	879
BbsI	GAAGAC	1 cut(s)	963
BbvI	GCAGC	5 cut(s)	125, 1213, 1250, 1382, 1387
BccI	CCATC	4 cut(s)	41, 826, 1190, 1445
BceAI	ACGGC	1 cut(s)	1358
BciT130I	CCWGG	3 cut(s)	984, 1260, 1284
BclI	TGATCA	1 cut(s)	643
BcnI	CCSGG	2 cut(s)	327, 1307
BcoDI	GTCTC	4 cut(s)	365, 586, 590, 1144
BfaI	CTAG	3 cut(s)	333, 1118, 1320
BfoI	RGCGCY	2 cut(s)	14, 33
BfuAI	ACCTGC	1 cut(s)	797
BisI	GCNGC	8 cut(s)	76, 114, 167, 804, 1227, 1239, 1371, 1401
BlsI	GCNGC	8 cut(s)	77, 115, 168, 805, 1228, 1240, 1372, 1402
Bme1390I	CCNGG	5 cut(s)	327, 984, 1260, 1284, 1307
Bme18I	GGWCC	3 cut(s)	127, 998, 1279
BmeRI	GACNNNNNGTC	1 cut(s)	378
BmeT110I	CYCGRG	1 cut(s)	185
BmgT120I	GGNCC	4 cut(s)	127, 875, 998, 1279
BmiI	GGNNCC	6 cut(s)	79, 454, 697, 1281, 1303, 1414
BmrFI	CCNGG	5 cut(s)	327, 984, 1260, 1284, 1307
BmsI	GCATC	2 cut(s)	267, 1357
BpiI	GAAGAC	1 cut(s)	963
BpmI	CTGGAG	1 cut(s)	1038
Bpu10I	CCTNAGC	1 cut(s)	117
BpuEI	CTTGAG	5 cut(s)	10, 191, 257, 1022, 1186
BpuMI	CCSGG	2 cut(s)	327, 1307
BsaAI	YACGTR	2 cut(s)	731, 1295
BsaI	GGTCTC	1 cut(s)	365
BsaJI	CCNNGG	6 cut(s)	991, 1258, 1259, 1275, 1282, 1305
BsaWI	WCCGGW	1 cut(s)	449
BsaXI	ACNNNNNCTCC	2 cut(s)	420, 450
Bsc4I	CCNNNNNNNGG	5 cut(s)	39, 530, 883, 1020, 1523
Bse1I	ACTGG	4 cut(s)	439, 484, 631, 699
Bse3DI	GCAATG	2 cut(s)	12, 230
BseAI	TCCGGA	1 cut(s)	449
BseBI	CCWGG	3 cut(s)	984, 1260, 1284
BseDI	CCNNGG	6 cut(s)	991, 1258, 1259, 1275, 1282, 1305
BseGI	GGATG	3 cut(s)	622, 1270, 1342
BseLI	CCNNNNNNNGG	5 cut(s)	39, 530, 883, 1020, 1523
BseMI	GCAATG	2 cut(s)	12, 230
BseMII	CTCAG	4 cut(s)	131, 360, 1099, 1458
BseNI	ACTGG	4 cut(s)	439, 484, 631, 699
BseXI	GCAGC	5 cut(s)	125, 1213, 1250, 1382, 1387
Bsh1236I	CGCG	1 cut(s)	390
BshFI	GGCC	3 cut(s)	43, 876, 1046
BshNI	GGYRCC	2 cut(s)	695, 1412
BsiHKCI	CYCGRG	1 cut(s)	185
BsiSI	CCGG	3 cut(s)	327, 450, 1307
BslFI	GGGAC	3 cut(s)	1196, 1241, 1265
BslI	CCNNNNNNNGG	5 cut(s)	39, 530, 883, 1020, 1523
BsmAI	GTCTC	4 cut(s)	365, 586, 590, 1144
BsmFI	GGGAC	3 cut(s)	1196, 1241, 1265
BsnI	GGCC	3 cut(s)	43, 876, 1046
Bso31I	GGTCTC	1 cut(s)	365
BsoBI	CYCGRG	1 cut(s)	185
Bsp13I	TCCGGA	1 cut(s)	449
Bsp1407I	TGTACA	1 cut(s)	474
Bsp143I	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
Bsp19I	CCATGG	1 cut(s)	1275
BspACI	CCGC	5 cut(s)	75, 96, 167, 803, 1095
BspANI	GGCC	3 cut(s)	43, 876, 1046
BspCNI	CTCAG	4 cut(s)	130, 361, 1098, 1459
BspEI	TCCGGA	1 cut(s)	449
BspFNI	CGCG	1 cut(s)	390
BspHI	TCATGA	2 cut(s)	297, 496
BspLI	GGNNCC	6 cut(s)	79, 454, 697, 1281, 1303, 1414
BspMI	ACCTGC	1 cut(s)	797
BspPI	GGATC	4 cut(s)	176, 407, 447, 460
BspT107I	GGYRCC	2 cut(s)	695, 1412
BspTNI	GGTCTC	1 cut(s)	365
BsrDI	GCAATG	2 cut(s)	12, 230
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	4 cut(s)	439, 484, 631, 699
BssECI	CCNNGG	6 cut(s)	991, 1258, 1259, 1275, 1282, 1305
BssMI	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
BssNAI	GTATAC	2 cut(s)	734, 1292
BssSI	CACGAG	1 cut(s)	879
BssT1I	CCWWGG	2 cut(s)	991, 1275
Bst1107I	GTATAC	2 cut(s)	734, 1292
Bst2BI	CACGAG	1 cut(s)	879
Bst2UI	CCWGG	3 cut(s)	984, 1260, 1284
Bst4CI	ACNGT	4 cut(s)	102, 270, 314, 1255
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	1 cut(s)	474
BstBAI	YACGTR	2 cut(s)	731, 1295
BstC8I	GCNNGC	5 cut(s)	20, 282, 712, 1044, 1115
BstDEI	CTNAG	4 cut(s)	117, 369, 1085, 1467
BstDSI	CCRYGG	1 cut(s)	1275
BstF5I	GGATG	3 cut(s)	622, 1270, 1342
BstFNI	CGCG	1 cut(s)	390
BstH2I	RGCGCY	2 cut(s)	14, 33
BstHHI	GCGC	3 cut(s)	13, 32, 392
BstKTI	GATC	7 cut(s)	184, 415, 455, 520, 646, 894, 1135
BstMAI	GTCTC	4 cut(s)	365, 586, 590, 1144
BstMBI	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
BstMWI	GCNNNNNNNGC	5 cut(s)	19, 398, 779, 1103, 1235
BstNI	CCWGG	3 cut(s)	984, 1260, 1284
BstNSI	RCATGY	1 cut(s)	94
BstSCI	CCNGG	5 cut(s)	325, 982, 1258, 1282, 1305
BstSNI	TACGTA	1 cut(s)	1295
BstUI	CGCG	1 cut(s)	390
BstV1I	GCAGC	5 cut(s)	125, 1213, 1250, 1382, 1387
BstV2I	GAAGAC	1 cut(s)	963
BstX2I	RGATCY	2 cut(s)	412, 452
BstYI	RGATCY	2 cut(s)	412, 452
BstZ17I	GTATAC	2 cut(s)	734, 1292
BsuRI	GGCC	3 cut(s)	43, 876, 1046
BtgI	CCRYGG	1 cut(s)	1275
BtsCI	GGATG	3 cut(s)	622, 1270, 1342
BtsI	GCAGTG	2 cut(s)	44, 63
BtsIMutI	CAGTG	9 cut(s)	44, 63, 98, 266, 319, 477, 624, 706, 796
BveI	ACCTGC	1 cut(s)	797
Cac8I	GCNNGC	5 cut(s)	20, 282, 712, 1044, 1115
CciI	TCATGA	2 cut(s)	297, 496
CfoI	GCGC	3 cut(s)	13, 32, 392
Cfr13I	GGNCC	4 cut(s)	127, 875, 998, 1279
Csp6I	GTAC	4 cut(s)	138, 310, 475, 1413
CviAII	CATG	9 cut(s)	91, 298, 364, 497, 508, 527, 545, 1276, 1487
CviQI	GTAC	4 cut(s)	138, 310, 475, 1413
DdeI	CTNAG	4 cut(s)	117, 369, 1085, 1467
DpnI	GATC	7 cut(s)	183, 414, 454, 519, 645, 893, 1134
DpnII	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
DraIII	CACNNNGTG	1 cut(s)	872
DriI	GACNNNNNGTC	1 cut(s)	378
Eam1105I	GACNNNNNGTC	1 cut(s)	378
Eco105I	TACGTA	1 cut(s)	1295
Eco130I	CCWWGG	2 cut(s)	991, 1275
Eco31I	GGTCTC	1 cut(s)	365
Eco32I	GATATC	3 cut(s)	199, 323, 573
Eco47I	GGWCC	3 cut(s)	127, 998, 1279
Eco57I	CTGAAG	2 cut(s)	234, 883
Eco88I	CYCGRG	1 cut(s)	185
EcoRII	CCWGG	3 cut(s)	982, 1258, 1282
EcoRV	GATATC	3 cut(s)	199, 323, 573
EcoT14I	CCWWGG	2 cut(s)	991, 1275
ErhI	CCWWGG	2 cut(s)	991, 1275
FaeI	CATG	9 cut(s)	94, 301, 367, 500, 511, 530, 548, 1279, 1490
FalI	AAGNNNNNCTT	2 cut(s)	1370, 1402
FaqI	GGGAC	3 cut(s)	1196, 1241, 1265
FatI	CATG	9 cut(s)	90, 297, 363, 496, 507, 526, 544, 1275, 1486
FbaI	TGATCA	1 cut(s)	643
FblI	GTMKAC	3 cut(s)	84, 733, 1291
Fnu4HI	GCNGC	8 cut(s)	76, 114, 167, 804, 1227, 1239, 1371, 1401
FokI	GGATG	3 cut(s)	629, 1277, 1349
Fsp4HI	GCNGC	8 cut(s)	76, 114, 167, 804, 1227, 1239, 1371, 1401
FspBI	CTAG	3 cut(s)	333, 1118, 1320
GlaI	GCGC	3 cut(s)	12, 31, 391
GluI	GCNGC	8 cut(s)	76, 114, 167, 804, 1227, 1239, 1371, 1401
GsuI	CTGGAG	1 cut(s)	1038
HaeII	RGCGCY	2 cut(s)	14, 33
HaeIII	GGCC	3 cut(s)	43, 876, 1046
HapII	CCGG	3 cut(s)	327, 450, 1307
HhaI	GCGC	3 cut(s)	13, 32, 392
Hin1II	CATG	9 cut(s)	94, 301, 367, 500, 511, 530, 548, 1279, 1490
Hin6I	GCGC	3 cut(s)	11, 30, 390
HinP1I	GCGC	3 cut(s)	11, 30, 390
HindIII	AAGCTT	2 cut(s)	399, 1065
HinfI	GANTC	3 cut(s)	504, 987, 1445
HpaII	CCGG	3 cut(s)	327, 450, 1307
HphI	GGTGA	1 cut(s)	286
Hpy166II	GTNNAC	8 cut(s)	85, 475, 477, 533, 734, 922, 1001, 1292
Hpy188I	TCNGA	5 cut(s)	253, 555, 674, 1450, 1468
Hpy8I	GTNNAC	8 cut(s)	85, 475, 477, 533, 734, 922, 1001, 1292
HpyAV	CCTTC	5 cut(s)	223, 258, 311, 898, 1117
HpyCH4III	ACNGT	4 cut(s)	102, 270, 314, 1255
HpyCH4IV	ACGT	2 cut(s)	730, 1294
HpyF10VI	GCNNNNNNNGC	5 cut(s)	19, 398, 779, 1103, 1235
HpyF3I	CTNAG	4 cut(s)	117, 369, 1085, 1467
HpySE526I	ACGT	2 cut(s)	730, 1294
Hsp92II	CATG	9 cut(s)	94, 301, 367, 500, 511, 530, 548, 1279, 1490
HspAI	GCGC	3 cut(s)	11, 30, 390
Kpn2I	TCCGGA	1 cut(s)	449
KpnI	GGTACC	1 cut(s)	1416
Ksp22I	TGATCA	1 cut(s)	643
Kzo9I	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
LmnI	GCTCC	1 cut(s)	83
Lsp1109I	GCAGC	5 cut(s)	125, 1213, 1250, 1382, 1387
LweI	GCATC	2 cut(s)	267, 1357
MaeI	CTAG	3 cut(s)	333, 1118, 1320
MaeII	ACGT	2 cut(s)	730, 1294
MaeIII	GTNAC	5 cut(s)	61, 629, 844, 864, 1088
MalI	GATC	7 cut(s)	183, 414, 454, 519, 645, 893, 1134
MboI	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
MboII	GAAGA	6 cut(s)	583, 886, 889, 968, 1390, 1402
MflI	RGATCY	2 cut(s)	412, 452
MluCI	AATT	7 cut(s)	256, 680, 963, 1100, 1174, 1191, 1511
MlyI	GAGTC	1 cut(s)	981
MmeI	TCCRAC	2 cut(s)	630, 1541
MnlI	CCTC	8 cut(s)	166, 338, 504, 534, 888, 1173, 1327, 1483
MroI	TCCGGA	1 cut(s)	449
MroXI	GAANNNNTTC	2 cut(s)	402, 1215
MseI	TTAA	4 cut(s)	683, 966, 1395, 1421
MslI	CAYNNNNRTG	2 cut(s)	362, 789
MspI	CCGG	3 cut(s)	327, 450, 1307
MspR9I	CCNGG	5 cut(s)	327, 984, 1260, 1284, 1307
MvaI	CCWGG	3 cut(s)	984, 1260, 1284
MvnI	CGCG	1 cut(s)	390
MwoI	GCNNNNNNNGC	5 cut(s)	19, 398, 779, 1103, 1235
NciI	CCSGG	2 cut(s)	327, 1307
NcoI	CCATGG	1 cut(s)	1275
NdeII	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
NlaIII	CATG	9 cut(s)	94, 301, 367, 500, 511, 530, 548, 1279, 1490
NlaIV	GGNNCC	6 cut(s)	79, 454, 697, 1281, 1303, 1414
NmeAIII	GCCGAG	1 cut(s)	199
NmuCI	GTSAC	2 cut(s)	61, 864
NspI	RCATGY	1 cut(s)	94
OliI	CACNNNNGTG	1 cut(s)	789
PagI	TCATGA	2 cut(s)	297, 496
PaqCI	CACCTGC	1 cut(s)	797
PasI	CCCWGGG	1 cut(s)	1259
PciI	ACATGT	1 cut(s)	90
PcsI	WCGNNNNNNNCGW	1 cut(s)	877
PdmI	GAANNNNTTC	2 cut(s)	402, 1215
PfeI	GAWTC	2 cut(s)	504, 1445
PflMI	CCANNNNNTGG	1 cut(s)	1523
PfoI	TCCNGGA	1 cut(s)	982
PkrI	GCNGC	8 cut(s)	77, 115, 168, 805, 1228, 1240, 1372, 1402
PleI	GAGTC	1 cut(s)	981
PpsI	GAGTC	1 cut(s)	981
Ppu21I	YACGTR	2 cut(s)	731, 1295
PscI	ACATGT	1 cut(s)	90
PshBI	ATTAAT	2 cut(s)	683, 966
Psp6I	CCWGG	3 cut(s)	982, 1258, 1282
PspGI	CCWGG	3 cut(s)	982, 1258, 1282
PspN4I	GGNNCC	6 cut(s)	79, 454, 697, 1281, 1303, 1414
PspPI	GGNCC	4 cut(s)	127, 875, 998, 1279
PsuI	RGATCY	2 cut(s)	412, 452
RsaI	GTAC	4 cut(s)	139, 311, 476, 1414
RsaNI	GTAC	4 cut(s)	138, 310, 475, 1413
RseI	CAYNNNNRTG	2 cut(s)	362, 789
SaqAI	TTAA	4 cut(s)	683, 966, 1395, 1421
SatI	GCNGC	8 cut(s)	76, 114, 167, 804, 1227, 1239, 1371, 1401
Sau3AI	GATC	7 cut(s)	181, 412, 452, 517, 643, 891, 1132
Sau96I	GGNCC	4 cut(s)	127, 875, 998, 1279
SchI	GAGTC	1 cut(s)	981
ScrFI	CCNGG	5 cut(s)	327, 984, 1260, 1284, 1307
SfaNI	GCATC	2 cut(s)	267, 1357
SinI	GGWCC	3 cut(s)	127, 998, 1279
SmiMI	CAYNNNNRTG	2 cut(s)	362, 789
SmlI	CTYRAG	5 cut(s)	25, 170, 236, 1037, 1201
SmoI	CTYRAG	5 cut(s)	25, 170, 236, 1037, 1201
SnaBI	TACGTA	1 cut(s)	1295
Sse9I	AATT	7 cut(s)	256, 680, 963, 1100, 1174, 1191, 1511
SsiI	CCGC	5 cut(s)	75, 96, 167, 803, 1095
SspMI	CTAG	3 cut(s)	333, 1118, 1320
StyD4I	CCNGG	5 cut(s)	325, 982, 1258, 1282, 1305
StyI	CCWWGG	2 cut(s)	991, 1275
TaaI	ACNGT	4 cut(s)	102, 270, 314, 1255
TaiI	ACGT	2 cut(s)	733, 1297
TaqI	TCGA	3 cut(s)	204, 584, 594
TasI	AATT	7 cut(s)	256, 680, 963, 1100, 1174, 1191, 1511
TatI	WGTACW	2 cut(s)	309, 474
TauI	GCSGC	3 cut(s)	78, 169, 806
TfiI	GAWTC	2 cut(s)	504, 1445
Tru1I	TTAA	4 cut(s)	683, 966, 1395, 1421
Tru9I	TTAA	4 cut(s)	683, 966, 1395, 1421
TscAI	CASTG	9 cut(s)	51, 70, 105, 273, 319, 484, 631, 706, 796
TseFI	GTSAC	2 cut(s)	61, 864
TseI	GCWGC	5 cut(s)	113, 1226, 1238, 1370, 1400
Tsp45I	GTSAC	2 cut(s)	61, 864
TspDTI	ATGAA	4 cut(s)	366, 513, 633, 969
TspGWI	ACGGA	1 cut(s)	70
TspRI	CASTG	9 cut(s)	51, 70, 105, 273, 319, 484, 631, 706, 796
Van91I	CCANNNNNTGG	1 cut(s)	1523
VpaK11BI	GGWCC	3 cut(s)	127, 998, 1279
VspI	ATTAAT	2 cut(s)	683, 966
XapI	RAATTY	4 cut(s)	256, 1100, 1174, 1511
XceI	RCATGY	1 cut(s)	94
XcmI	CCANNNNNNNNNTGG	1 cut(s)	476
XmiI	GTMKAC	3 cut(s)	84, 733, 1291
XmnI	GAANNNNTTC	2 cut(s)	402, 1215
XspI	CTAG	3 cut(s)	333, 1118, 1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.