MD15G1415700.v1.1
NAC Family

NAC transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003637
Physical Location & Seq
Forward (+)
51689615 .. 51691311
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1415700.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGGAGACGACCAGTTTAAGCTCCCTCCTGGTTTCCGATTTGACCCGACCGACGAAGAACTTGTAGTCCATTTCCTTCAACGTAAGGCAGCTCTCTTACCTTTCCATCCCGATGTCATTCCCGATCTCCATCTCTACCCATATGATCCATGGGAATTAAATGGTAAGGCTTTGAGTGAGGGCAAGCAGTGGTACTTCTACAGCAGGAGGGCACAGAATCGGGTCACGAATAATGGGTACTGGAAAACATTAGGCATTGAAGAACCTATCTTTTCAAGCTCATCTTCTAATCCTACCAAGAAAGTTGGGATGAAAAAATATTTGACGTTTTTCGTTGGAGAAGCTCCAAATTCAGGCATCAAAACTAATTGGACAATGCACGAGTATCGTCTCTCTTCGTCAGCCTCAGATTGTGCTTCTTCTTCCACCACCACGACCACCAGCAGATCATCCAAAAGAAGAGGGCAATCCAATACAGAATACAGTGGATACGTTCTGTGTAAAGTCTACGAGCGTGAGGATGATGATGATGATGGGACAGAGCTTTCGTGCCTGGACGAAGTGTTCTTATCTTTGGATGATCTTGATGAAATAAGTTTGCCAAATTATAATTAG

Protein Analysis

205

Amino Acids

23.3

Weight (kDa)

4.95

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 131 4.8e-35 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AccI GTMKAC 1 cut(s) 507
AclWI GGATC 1 cut(s) 140
AcsI RAATTY 1 cut(s) 349
AfaI GTAC 2 cut(s) 194, 239
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 3 cut(s) 80, 260, 276
AjnI CCWGG 2 cut(s) 28, 552
AloI GAACNNNNNNTCC 4 cut(s) 51, 83, 548, 580
AluBI AGCT 5 cut(s) 22, 92, 279, 344, 544
AluI AGCT 5 cut(s) 22, 92, 279, 344, 544
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 349
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
BaeGI GKGCMC 1 cut(s) 214
BauI CACGAG 1 cut(s) 380
BbvI GCAGC 1 cut(s) 101
BccI CCATC 3 cut(s) 114, 138, 527
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 2 cut(s) 30, 554
BciVI GTATCC 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 199
BfuI GTATCC 1 cut(s) 482
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 30, 554
BmrFI CCNGG 2 cut(s) 30, 554
BmsI GCATC 1 cut(s) 366
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 2 cut(s) 13, 245
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 2 cut(s) 30, 554
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 5 cut(s) 106, 315, 449, 526, 583
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 13, 245
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 51
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 395
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 4 cut(s) 124, 145, 446, 580
Bsp19I CCATGG 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 419
BspPI GGATC 1 cut(s) 140
BsrI ACTGG 2 cut(s) 13, 245
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 4 cut(s) 124, 145, 446, 580
BssSI CACGAG 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 380
Bst2UI CCWGG 2 cut(s) 30, 554
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 2 cut(s) 400, 454
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 406
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 5 cut(s) 106, 315, 449, 526, 583
BstKTI GATC 4 cut(s) 127, 148, 449, 583
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 4 cut(s) 124, 145, 446, 580
BstMCI CGRYCG 1 cut(s) 51
BstNI CCWGG 2 cut(s) 30, 554
BstSCI CCNGG 2 cut(s) 28, 552
BstSFI CTRYAG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 101
BsuI GTATCC 1 cut(s) 482
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 5 cut(s) 106, 315, 449, 526, 583
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 2 cut(s) 194, 490
Cac8I GCNNGC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 193, 238
CviAII CATG 1 cut(s) 150
CviJI RGCY 7 cut(s) 22, 92, 170, 279, 344, 404, 544
CviKI_1 RGCY 7 cut(s) 22, 92, 170, 279, 344, 404, 544
CviQI GTAC 2 cut(s) 193, 238
DdeI CTNAG 1 cut(s) 406
DpnI GATC 4 cut(s) 126, 147, 448, 582
DpnII GATC 4 cut(s) 124, 145, 446, 580
Eam1104I CTCTTC 2 cut(s) 400, 454
EarI CTCTTC 2 cut(s) 400, 454
Eco130I CCWWGG 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 28, 552
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 1 cut(s) 153
FaiI YATR 4 cut(s) 142, 144, 151, 609
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FaqI GGGAC 1 cut(s) 550
FatI CATG 1 cut(s) 149
FauNDI CATATG 1 cut(s) 142
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 5 cut(s) 93, 322, 436, 533, 590
Fsp4HI GCNGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 90
Hin1II CATG 1 cut(s) 153
HinfI GANTC 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 2 cut(s) 38, 409
Hpy188III TCNNGA 4 cut(s) 110, 122, 226, 584
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 3 cut(s) 82, 326, 492
HpyCH4V TGCA 1 cut(s) 379
HpyF3I CTNAG 1 cut(s) 406
HpySE526I ACGT 3 cut(s) 82, 326, 492
Hsp92II CATG 1 cut(s) 153
Kzo9I GATC 4 cut(s) 124, 145, 446, 580
LmnI GCTCC 2 cut(s) 27, 349
LpnPI CCDG 9 cut(s) 15, 26, 42, 190, 226, 339, 454, 539, 566
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 1 cut(s) 366
MaeII ACGT 3 cut(s) 82, 326, 492
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 126, 147, 448, 582
MboI GATC 4 cut(s) 124, 145, 446, 580
MboII GAAGA 7 cut(s) 68, 272, 276, 387, 411, 414, 471
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 5 cut(s) 155, 349, 367, 604, 610
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 6 cut(s) 36, 172, 201, 415, 455, 511
MseI TTAA 2 cut(s) 18, 158
MslI CAYNNNNRTG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 30, 554
MvaI CCWGG 2 cut(s) 30, 554
NcoI CCATGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 124, 145, 446, 580
NlaIII CATG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 217
PkrI GCNGC 1 cut(s) 91
PsiI TTATAA 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 28, 552
PspGI CCWGG 2 cut(s) 28, 552
RsaI GTAC 2 cut(s) 194, 239
RsaNI GTAC 2 cut(s) 193, 238
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 2 cut(s) 18, 158
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 4 cut(s) 124, 145, 446, 580
ScrFI CCNGG 2 cut(s) 30, 554
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 111
Sse9I AATT 5 cut(s) 155, 349, 367, 604, 610
SspI AATATT 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 28, 552
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 3 cut(s) 85, 329, 495
TaqII GACCGA 1 cut(s) 65
TasI AATT 5 cut(s) 155, 349, 367, 604, 610
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 2 cut(s) 18, 158
Tru9I TTAA 2 cut(s) 18, 158
TscAI CASTG 2 cut(s) 194, 490
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 326, 603
TspRI CASTG 2 cut(s) 194, 490
XapI RAATTY 1 cut(s) 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.