Rorug07G0091500
NAC Family

acid phosphatase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003637
Physical Location & Seq
Forward (+)
7158895 .. 7164342
5448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0091500.1

Sequence Viewer

Length: 2217 bp
ATGGCGCACGTTAGCCAGCTTCCTTGCGACGGAGACGGCGTCTGCATGCTCTGCAAAACGACGCCGTCGCACGAGGACCAGCTCACCTGCAGGACCTGCGTCACCCCGTGGCACGTCAGCTGCCTCTCGTCCCGTCCCGAATCGCTGGCTTCCACTCTCCAGTGGGAGTGCCCAGACTGTTCCTCGCTCTCCGGCGACACGCCGGCCGTGAAATCGGCCGTCTCCGGCGAGCTGATCGCCGCGATCCGGGCGATCGAGGCCGATGCGGCGCTGACGGAGCAGCAGAAGGCGAGTAAGCGACAGGAGCTGATGAGCGGGAAGGCTCTGCCGGAGGAAGAAGAAGGCGCCGGCAGGAAGGGAGTAAACGACGGCGTTTTGGATGGGATTTTCAATTGCTCGTTCTGTATGCAACTACCGGAGAGACCTGTTACGACACCATGTGGCCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCCATGCCATTCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGTTGCCATACGTATGGCTAAGATGTCAAAATCATATGTTACTGAGGGGCCTGCTAAGGTATACCACTTTGTCCGAAACCAAGACAGACCTGACAAAGCTTTCACTACCGAGCGGGCGCAGAAAACTGGGAAATCAAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGACCTATACCTGCTGAATATGATCCTGAGAGAAACCAAGGTGTGCTGGTAGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGATCAGTCATTTGACCAGACATTTGAGAAATATAACAAGGCTCTCCAAGTTAGTTGCCTGAAAGGGTATCCTGTACGGGTGGTAAGATCGCACAAGGAGAAGCGCTCCTCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAGTCTATAGAATTGAAAAATGTTGGACAAAGGTGGGAATCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTTAGATGTGACAATGAACCTGCACCATGGACGAGTGATGAACATGGGGACCGGCCTAGACCTTTACCTCCAATACCTGAGCTGAAGAAAGCTACAAGTATAATAGAGAGGAAGTCAAGTCCATCGTGGGACTTTGAGGAGAAAGATGGCTGCTGGAAGTGGAAAAAGTCACCACCTATCAGTAAAAAAACCGTTCAGACATTAGATTCTGCTGATCGAAAGAGGTCAAGAAAAGGAAAGAGGAAGGCAAAAAATATTACTGTCCGAGAGAGGCTCTTAAGAGAATTCAGCTGCCTTGTTTGCGGGGAGGTCATGACATTACCAATGACCACACCTTGTGCTCATAACTTTTGCAAATCCTGTTTGGAAGGCGCTTTTGCAGGAAAGAGCTTGGTGAGAGAAAGAAACAGAGGGGGAAGAACCCTTCGAACCCAAAAGAATATAATGAGCTGCCCTTCTTGTCCTACTGATATCTCTGATTTTCTTCAAGATCCTCAGGTGAATAGAGAGTTAATGGATGCGATTGAGTCACTTAAGTCACAGAATGAAGAGGAAGAGAATGAGGATTCAGTTGAAGAAATGAGTGAAGATGTGGATAATTCTGACAACGAAATTAGCACTGAAATGGTTGAAGAAGCAGGTGTGAAAGAATGTGCTGAGGGTTCTCCAACAAACTGCAATCCCGAAAGGAGTCCCAAACGACAGAAAGTTGATGCTGGTGAAGGCTCACCAAAAACCAATGATGAGGAGGTAAATTGCATTCCTGTCGCCCAGACTAATGGCAAAGGCTCACCGGAAACCGATACTGAAGAGGTAAATGAAACTCCTGTCGCCCATACTAATGGCAAGGGCTCACCGGCTCCAGTTGCAAAATGTGGCAAGCAAGATGCTGATCCGGTTGGCGTTGCAGCAAATACTAGGAGGAGGAAGGCTGAAGTAGTGGGTAAAGATAATGGTTCCCCATCAAGTCCTCTGCTTGTGCAATCTGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

738

Amino Acids

81.41

Weight (kDa)

6.93

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 131 - 174 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 131 - 171 1.6e-06 Ring finger domain
zf-C3HC4 PF00097 132 - 170 3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 170 3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 132 - 170 5.2e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 262 - 411 1.4e-49 SAD/SRA domain
zf-RING_UBOX PF13445 504 - 535 4.8e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 95, 1847
Acc36I ACCTGC 5 cut(s) 95, 104, 769, 1246, 1847
Acc65I GGTACC 1 cut(s) 918
AccB1I GGYRCC 2 cut(s) 344, 918
AccBSI CCGCTC 2 cut(s) 315, 679
AccI GTMKAC 2 cut(s) 627, 832
AccII CGCG 1 cut(s) 242
AciI CCGC 8 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521
AclWI GGATC 6 cut(s) 238, 767, 942, 1014, 1703, 2105
AcoI YGGCCR 3 cut(s) 204, 216, 442
AcsI RAATTY 1 cut(s) 1502
AcuI CTGAAG 3 cut(s) 1322, 2046, 2172
AcyI GRCGYC 3 cut(s) 39, 62, 345
AdeI CACNNNGTG 3 cut(s) 108, 440, 1556
AfaI GTAC 3 cut(s) 496, 920, 1080
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
AflII CTTAAG 2 cut(s) 1495, 1751
AgsI TTSAA 8 cut(s) 391, 460, 827, 1163, 1706, 1793, 1850, 2213
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 1188
Alw21I GWGCWC 3 cut(s) 847, 895, 1561
Alw26I GTCTC 3 cut(s) 27, 226, 415
Alw44I GTGCAC 2 cut(s) 843, 891
AlwI GGATC 6 cut(s) 238, 767, 942, 1014, 1703, 2105
AlwNI CAGNNNCTG 2 cut(s) 96, 307
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 6 cut(s) 204, 216, 258, 442, 614, 1271
ApaLI GTGCAC 2 cut(s) 843, 891
ApeKI GCWGC 7 cut(s) 120, 280, 707, 1368, 1509, 1668, 2126
ApoI RAATTY 1 cut(s) 1502
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 918
AspLEI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
AspS9I GGNCC 5 cut(s) 76, 93, 614, 752, 1267
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 9 cut(s) 76, 94, 1380, 1624, 1729, 1938, 1949, 2001, 2064
AsuII TTCGAA 1 cut(s) 1645
AvaII GGWCC 4 cut(s) 76, 93, 752, 1267
AxyI CCTNAGG 1 cut(s) 1713
BaeGI GKGCMC 3 cut(s) 173, 847, 895
BalI TGGCCA 1 cut(s) 444
BanI GGYRCC 2 cut(s) 344, 918
BanII GRGCYC 1 cut(s) 2072
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 847, 895, 1561
BbvCI CCTCAGC 1 cut(s) 1875
BbvI GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BccI CCATC 5 cut(s) 374, 1139, 1348, 1358, 2188
BceAI ACGGC 5 cut(s) 49, 52, 191, 203, 385
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1966, 2000
BciT130I CCWGG 1 cut(s) 1190
BciVI GTATCC 1 cut(s) 1083
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 27, 226, 415
BfaI CTAG 2 cut(s) 1275, 2136
BfmI CTRYAG 2 cut(s) 88, 1153
BfoI RGCGCY 4 cut(s) 272, 348, 1111, 1593
BfrI CTTAAG 2 cut(s) 1495, 1751
BfuAI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1847
BfuI GTATCC 1 cut(s) 1083
BglI GCCNNNNNGGC 1 cut(s) 266
Bme1390I CCNGG 2 cut(s) 248, 1190
Bme18I GGWCC 4 cut(s) 76, 93, 752, 1267
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 5 cut(s) 76, 93, 614, 752, 1267
BmiI GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2176
BmrFI CCNGG 2 cut(s) 248, 1190
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
BmuI ACTGGG 1 cut(s) 702
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 1476, 1508
BpmI CTGGAG 2 cut(s) 143, 2064
Bpu10I CCTNAGC 3 cut(s) 621, 1296, 1875
Bpu14I TTCGAA 1 cut(s) 1645
BpuMI CCSGG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 2 cut(s) 933, 2109
BsaHI GRCGYC 3 cut(s) 39, 62, 345
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BsaWI WCCGGW 3 cut(s) 415, 2011, 2113
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
Bse118I RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
Bse1I ACTGG 3 cut(s) 160, 697, 2081
Bse21I CCTNAGG 1 cut(s) 1713
Bse8I GATNNNNATC 2 cut(s) 933, 2109
BseBI CCWGG 1 cut(s) 1190
BseDI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BseGI GGATG 2 cut(s) 385, 1741
BseJI GATNNNNATC 2 cut(s) 933, 2109
BseLI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BseMII CTCAG 6 cut(s) 600, 768, 1116, 1287, 1727, 1866
BseNI ACTGG 3 cut(s) 160, 697, 2081
BseRI GAGGAG 4 cut(s) 1102, 1370, 1979, 2155
BseSI GKGCMC 3 cut(s) 173, 847, 895
BseX3I CGGCCG 2 cut(s) 204, 216
BseXI GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 1224
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 3 cut(s) 207, 219, 255
BshFI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BshNI GGYRCC 2 cut(s) 344, 918
BsiEI CGRYCG 3 cut(s) 207, 219, 255
BsiHKAI GWGCWC 3 cut(s) 847, 895, 1561
BslFI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
BslI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BsmAI GTCTC 3 cut(s) 27, 226, 415
BsmBI CGTCTC 2 cut(s) 27, 226
BsmFI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
BsmI GAATGC 2 cut(s) 812, 1977
BsnI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
Bso31I GGTCTC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 1645
Bsp1286I GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
Bsp19I CCATGG 2 cut(s) 886, 1244
BspACI CCGC 8 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521
BspANI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BspCNI CTCAG 6 cut(s) 601, 769, 1117, 1288, 1726, 1867
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 1530
BspLI GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2176
BspMAI CTGCAG 1 cut(s) 92
BspMI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1847
BspPI GGATC 6 cut(s) 238, 767, 942, 1014, 1703, 2105
BspT104I TTCGAA 1 cut(s) 1645
BspT107I GGYRCC 2 cut(s) 344, 918
BspTI CTTAAG 2 cut(s) 1495, 1751
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 315, 679
BsrFI RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
BsrI ACTGG 3 cut(s) 160, 697, 2081
BssAI RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
BssECI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BssNAI GTATAC 1 cut(s) 628
BssNI GRCGYC 3 cut(s) 39, 62, 345
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 787, 886, 1244
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1190
Bst4CI ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
Bst6I CTCTTC 3 cut(s) 1761, 1767, 2022
BstACI GRCGYC 3 cut(s) 39, 62, 345
BstAFI CTTAAG 2 cut(s) 1495, 1751
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 578
BstBI TTCGAA 1 cut(s) 1645
BstC8I GCNNGC 9 cut(s) 17, 47, 147, 204, 230, 349, 618, 681, 2099
BstDEI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
BstDSI CCRYGG 3 cut(s) 107, 886, 1244
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 2 cut(s) 385, 1741
BstFNI CGCG 1 cut(s) 242
BstH2I RGCGCY 4 cut(s) 272, 348, 1111, 1593
BstHHI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
BstMAI GTCTC 3 cut(s) 27, 226, 415
BstMCI CGRYCG 3 cut(s) 207, 219, 255
BstNI CCWGG 1 cut(s) 1190
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 246, 1188
BstSFI CTRYAG 2 cut(s) 88, 1153
BstSLI GKGCMC 3 cut(s) 173, 847, 895
BstSNI TACGTA 1 cut(s) 578
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BstX2I RGATCY 1 cut(s) 1708
BstXI CCANNNNNNTGG 4 cut(s) 580, 863, 1997, 2060
BstYI RGATCY 1 cut(s) 1708
BstZ17I GTATAC 1 cut(s) 628
BstZI CGGCCG 2 cut(s) 204, 216
Bsu36I CCTNAGG 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BtgI CCRYGG 3 cut(s) 107, 886, 1244
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 385, 1741
BtsIMutI CAGTG 3 cut(s) 167, 842, 1836
BveI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1847
Cac8I GCNNGC 9 cut(s) 17, 47, 147, 204, 230, 349, 618, 681, 2099
CaiI CAGNNNCTG 2 cut(s) 96, 307
CciI TCATGA 1 cut(s) 1530
CfoI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
Cfr10I RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
Cfr13I GGNCC 5 cut(s) 76, 93, 614, 752, 1267
CseI GACGC 3 cut(s) 28, 70, 88
Csp6I GTAC 3 cut(s) 495, 919, 1079
CviAII CATG 9 cut(s) 46, 438, 515, 857, 887, 938, 1245, 1262, 1531
CviQI GTAC 3 cut(s) 495, 919, 1079
DdeI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
DinI GGCGCC 1 cut(s) 346
DraIII CACNNNGTG 3 cut(s) 108, 440, 1556
EaeI YGGCCR 3 cut(s) 204, 216, 442
EagI CGGCCG 2 cut(s) 204, 216
Eam1104I CTCTTC 3 cut(s) 1761, 1767, 2022
EarI CTCTTC 3 cut(s) 1761, 1767, 2022
EclXI CGGCCG 2 cut(s) 204, 216
Eco105I TACGTA 1 cut(s) 578
Eco130I CCWWGG 3 cut(s) 787, 886, 1244
Eco24I GRGCYC 1 cut(s) 2072
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 1690
Eco47I GGWCC 4 cut(s) 76, 93, 752, 1267
Eco47III AGCGCT 1 cut(s) 1109
Eco52I CGGCCG 2 cut(s) 204, 216
Eco57I CTGAAG 3 cut(s) 1322, 2046, 2172
Eco81I CCTNAGG 1 cut(s) 1713
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 93, 614
EcoRI GAATTC 1 cut(s) 1502
EcoRII CCWGG 1 cut(s) 1188
EcoRV GATATC 1 cut(s) 1690
EcoT14I CCWWGG 3 cut(s) 787, 886, 1244
EcoT38I GRGCYC 1 cut(s) 2072
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 3 cut(s) 787, 886, 1244
Esp3I CGTCTC 2 cut(s) 27, 226
FaeI CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
FaqI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
FatI CATG 9 cut(s) 45, 437, 514, 856, 886, 937, 1244, 1261, 1530
FauI CCCGC 4 cut(s) 308, 554, 672, 1514
FauNDI CATATG 2 cut(s) 601, 1117
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 2 cut(s) 627, 832
FokI GGATG 2 cut(s) 392, 1748
FriOI GRGCYC 1 cut(s) 2072
FspBI CTAG 2 cut(s) 1275, 2136
GlaI GCGC 6 cut(s) 6, 270, 346, 684, 1109, 1591
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 143, 2064
HaeII RGCGCY 4 cut(s) 272, 348, 1111, 1593
HaeIII GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
HgaI GACGC 3 cut(s) 28, 70, 88
HhaI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
Hin1I GRCGYC 3 cut(s) 39, 62, 345
Hin1II CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
Hin6I GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
HinP1I GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
HincII GTYRAC 2 cut(s) 542, 833
HindII GTYRAC 2 cut(s) 542, 833
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 7 cut(s) 140, 1149, 1185, 1424, 1745, 1784, 1909
HphI GGTGA 9 cut(s) 76, 94, 1380, 1624, 1729, 1938, 1949, 2001, 2064
Hpy166II GTNNAC 6 cut(s) 364, 542, 628, 833, 845, 893
Hpy188I TCNGA 6 cut(s) 641, 1416, 1484, 1696, 1822, 2206
Hpy188III TCNNGA 8 cut(s) 137, 469, 776, 912, 1446, 1531, 1706, 1901
Hpy8I GTNNAC 6 cut(s) 364, 542, 628, 833, 845, 893
Hpy99I CGWCG 4 cut(s) 32, 64, 70, 371
HpyCH4III ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
HpyCH4IV ACGT 3 cut(s) 9, 114, 577
HpyF3I CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
HpySE526I ACGT 3 cut(s) 9, 114, 577
Hsp92I GRCGYC 3 cut(s) 39, 62, 345
Hsp92II CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
HspAI GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
KasI GGCGCC 1 cut(s) 344
KpnI GGTACC 1 cut(s) 922
KroI GCCGGC 2 cut(s) 202, 347
KroNI GCCGGC 2 cut(s) 204, 349
Ksp22I TGATCA 1 cut(s) 742
LmnI GCTCC 4 cut(s) 277, 304, 1115, 2083
Lsp1109I GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
LweI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
MaeI CTAG 2 cut(s) 1275, 2136
MaeII ACGT 3 cut(s) 9, 114, 577
MaeIII GTNAC 8 cut(s) 100, 427, 604, 727, 1226, 1386, 1746, 1755
MbiI CCGCTC 2 cut(s) 315, 679
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 1708
MhlI GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
MluNI TGGCCA 1 cut(s) 444
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 3 cut(s) 1158, 1754, 1918
MmeI TCCRAC 3 cut(s) 460, 1151, 1910
Mox20I TGGCCA 1 cut(s) 444
MroNI GCCGGC 2 cut(s) 202, 347
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 444
MseI TTAA 4 cut(s) 1197, 1496, 1730, 1752
MslI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 3 cut(s) 120, 740, 1509
MspCI CTTAAG 2 cut(s) 1495, 1751
MspR9I CCNGG 2 cut(s) 248, 1190
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 2 cut(s) 812, 1977
MvaI CCWGG 1 cut(s) 1190
MvnI CGCG 1 cut(s) 242
NaeI GCCGGC 2 cut(s) 204, 349
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 1 cut(s) 248
NcoI CCATGG 2 cut(s) 886, 1244
NdeI CATATG 2 cut(s) 601, 1117
NgoMIV GCCGGC 2 cut(s) 202, 347
NlaIII CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
NlaIV GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2176
NmuCI GTSAC 6 cut(s) 100, 727, 1226, 1386, 1746, 1755
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 1645
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 1 cut(s) 1530
PaqCI CACCTGC 2 cut(s) 95, 1847
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 2 cut(s) 812, 1977
PdiI GCCGGC 2 cut(s) 204, 349
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 4 cut(s) 140, 1185, 1424, 1784
PflFI GACNNNGTC 2 cut(s) 38, 64
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 3 cut(s) 1157, 1753, 1917
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 3 cut(s) 1157, 1753, 1917
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 1188
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 1 cut(s) 1188
PspN4I GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2176
PspPI GGNCC 5 cut(s) 76, 93, 614, 752, 1267
PspPPI RGGWCCY 1 cut(s) 93
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 96, 307
PsuI RGATCY 1 cut(s) 1708
PsyI GACNNNGTC 2 cut(s) 38, 64
PvuI CGATCG 1 cut(s) 255
PvuII CAGCTG 3 cut(s) 120, 740, 1509
RsaI GTAC 3 cut(s) 496, 920, 1080
RsaNI GTAC 3 cut(s) 495, 919, 1079
RseI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
SalI GTCGAC 1 cut(s) 831
SaqAI TTAA 4 cut(s) 1197, 1496, 1730, 1752
Sau96I GGNCC 5 cut(s) 76, 93, 614, 752, 1267
SbfI CCTGCAGG 1 cut(s) 92
SchI GAGTC 3 cut(s) 1158, 1754, 1918
ScrFI CCNGG 2 cut(s) 248, 1190
SdaI CCTGCAGG 1 cut(s) 92
SduI GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
SfaNI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
SfcI CTRYAG 2 cut(s) 88, 1153
SfoI GGCGCC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 1645
SinI GGWCC 4 cut(s) 76, 93, 752, 1267
SmiMI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
SmlI CTYRAG 2 cut(s) 1495, 1751
SmoI CTYRAG 2 cut(s) 1495, 1751
SnaBI TACGTA 1 cut(s) 578
SphI GCATGC 1 cut(s) 49
Sse8387I CCTGCAGG 1 cut(s) 92
Sse9I AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
SsiI CCGC 8 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521
SspDI GGCGCC 1 cut(s) 344
SspI AATATT 1 cut(s) 1474
SspMI CTAG 2 cut(s) 1275, 2136
StyD4I CCNGG 2 cut(s) 246, 1188
StyI CCWWGG 3 cut(s) 787, 886, 1244
TaaI ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
TaiI ACGT 3 cut(s) 12, 117, 580
TaqI TCGA 4 cut(s) 255, 832, 1435, 1645
TasI AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
TauI GCSGC 3 cut(s) 242, 269, 513
TfiI GAWTC 4 cut(s) 140, 1185, 1424, 1784
Tru1I TTAA 4 cut(s) 1197, 1496, 1730, 1752
Tru9I TTAA 4 cut(s) 1197, 1496, 1730, 1752
TscAI CASTG 3 cut(s) 167, 842, 1843
TseFI GTSAC 6 cut(s) 100, 727, 1226, 1386, 1746, 1755
TseI GCWGC 7 cut(s) 120, 280, 707, 1368, 1509, 1668, 2126
Tsp45I GTSAC 6 cut(s) 100, 727, 1226, 1386, 1746, 1755
TspDTI ATGAA 4 cut(s) 1248, 1272, 1779, 2052
TspGWI ACGGA 2 cut(s) 45, 290
TspRI CASTG 3 cut(s) 167, 842, 1843
Tth111I GACNNNGTC 2 cut(s) 38, 64
Vha464I CTTAAG 2 cut(s) 1495, 1751
VneI GTGCAC 2 cut(s) 843, 891
VpaK11BI GGWCC 4 cut(s) 76, 93, 752, 1267
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 1 cut(s) 1502
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 627, 832
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 2 cut(s) 1275, 2136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.