MD16G1006000.v1.1

Domain of unknown function (DUF3067)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007081
Physical Location & Seq
Reverse (-)
478692 .. 480669
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1006000.v1.1.491

Sequence Viewer

Length: 669 bp
ATGACGAGCATAATTTCAAGTCACCGTTGTAATTGGTCGTTTGGAGTTCAGGAGACTGTTAACGTAGGAAAAGGATTCAACAAGGTTTTGTTCCCGGGATGTAATGGTTCATACTTCCCTTCAAGAGGATTGGGTTTTTCCACCGGTAATTGCCACATTCGTCATCAAAGTAATCGGAGACATAGGCCCATCTTTTCGAGTACAACAGATGATGGCAACGACATAAATCCAGATGACTCGGAAGATACAAGTGATGCAAAAGAAACCGGAGGGGTGGATAATAATGAAATGCTGAGAGAGAATCTTGAAAGACTGGTTGGTAGAGATGATTCCGCGTTTAGCGGGATAGACCTCGCAACTCTTATTAGGAACAAGTACGGCAGGTCCTATGACGTTCAACTCATCAAGAAGGAGTTCCTTGGAAGAAATCTCCTTGCTTTGAATGTCATGTGGAAGTATATGGAGCAGAGATCGTTTCCACTGACGGAAGAAGAGTATCTGTTGAGGCTCGACGACGTTGCAAACACGTTGAAATGCTGGGGAGCTGTGTCTCATATCCGAAACAGCCTAGCAACGGTGAAAGAGAGGCCTCGGATAGGAAAGGCAGTTAGCATTTTCATAGACATGGACGAGTCTGGAGGGCGTGCAAATGAATGGATTTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3067

Protein Analysis

223

Amino Acids

25.15

Weight (kDa)

6.53

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3067 PF11267 117 - 220 3.9e-35 Domain of unknown function (DUF3067)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccII CGCG 1 cut(s) 335
AciI CCGC 2 cut(s) 333, 342
AfaI GTAC 2 cut(s) 202, 377
AfiI CCNNNNNNNGG 3 cut(s) 125, 574, 596
AflIII ACRYGT 1 cut(s) 525
AgeI ACCGGT 1 cut(s) 143
AgsI TTSAA 7 cut(s) 18, 79, 123, 308, 398, 442, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 1 cut(s) 545
AluI AGCT 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 47, 172, 555
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 2 cut(s) 185, 587
AsiGI ACCGGT 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 186, 384
AsuC2I CCSGG 2 cut(s) 95, 96
AsuHPI GGTGA 2 cut(s) 14, 589
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 384
BccI CCATC 2 cut(s) 197, 206
BceAI ACGGC 1 cut(s) 394
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BcnI CCSGG 2 cut(s) 95, 96
BcoDI GTCTC 3 cut(s) 47, 172, 555
BfaI CTAG 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 372
Bme1390I CCNGG 2 cut(s) 95, 96
Bme18I GGWCC 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 186, 384
BmrFI CCNGG 2 cut(s) 95, 96
BmsI GCATC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 95, 96
BsaJI CCNNGG 3 cut(s) 94, 418, 590
BsaWI WCCGGW 2 cut(s) 143, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 574, 596
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 318
BseDI CCNNGG 3 cut(s) 94, 418, 590
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 3 cut(s) 125, 574, 596
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 318
BseYI CCCAGC 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 2 cut(s) 187, 589
BshTI ACCGGT 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 3 cut(s) 95, 144, 267
BslI CCNNNNNNNGG 3 cut(s) 125, 574, 596
BsmAI GTCTC 3 cut(s) 47, 172, 555
BsnI GGCC 2 cut(s) 187, 589
BsoBI CYCGRG 1 cut(s) 94
Bsp143I GATC 1 cut(s) 470
BspACI CCGC 2 cut(s) 333, 342
BspANI GGCC 2 cut(s) 187, 589
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 335
BspMI ACCTGC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 318
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 94, 418, 590
BssMI GATC 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 3 cut(s) 26, 58, 577
Bst6I CTCTTC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 1 cut(s) 293
BstENI CCTNNNNNAGG 2 cut(s) 123, 594
BstF5I GGATG 1 cut(s) 104
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 1 cut(s) 473
BstMAI GTCTC 3 cut(s) 47, 172, 555
BstMBI GATC 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 93, 94
BstUI CGCG 1 cut(s) 335
BsuRI GGCC 2 cut(s) 187, 589
BtsCI GGATG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 479
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 645
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 186, 384
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 2 cut(s) 201, 376
CspAI ACCGGT 1 cut(s) 143
CviAII CATG 2 cut(s) 448, 625
CviJI RGCY 5 cut(s) 187, 508, 545, 567, 589
CviKI_1 RGCY 5 cut(s) 187, 508, 545, 567, 589
CviQI GTAC 2 cut(s) 201, 376
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 472
DpnII GATC 1 cut(s) 470
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 418
Eco147I AGGCCT 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 94
EcoNI CCTNNNNNAGG 2 cut(s) 123, 594
EcoO109I RGGNCCY 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 2 cut(s) 451, 628
FatI CATG 2 cut(s) 447, 624
FauI CCCGC 1 cut(s) 335
FokI GGATG 1 cut(s) 111
FspBI CTAG 1 cut(s) 569
GsaI CCCAGC 1 cut(s) 541
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 2 cut(s) 187, 589
HapII CCGG 3 cut(s) 95, 144, 267
Hin1II CATG 2 cut(s) 451, 628
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 5 cut(s) 75, 236, 301, 329, 632
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 3 cut(s) 95, 144, 267
HphI GGTGA 2 cut(s) 14, 589
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 4 cut(s) 177, 241, 560, 594
Hpy188III TCNNGA 6 cut(s) 50, 123, 230, 305, 406, 636
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 2 cut(s) 515, 518
HpyAV CCTTC 2 cut(s) 129, 403
HpyCH4III ACNGT 3 cut(s) 26, 58, 577
HpyCH4IV ACGT 4 cut(s) 63, 393, 516, 527
HpyCH4V TGCA 3 cut(s) 257, 521, 647
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 4 cut(s) 63, 393, 516, 527
Hsp92II CATG 2 cut(s) 451, 628
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 470
LmnI GCTCC 2 cut(s) 463, 542
LpnPI CCDG 9 cut(s) 35, 108, 157, 243, 280, 299, 367, 523, 621
LweI GCATC 1 cut(s) 244
MaeI CTAG 1 cut(s) 569
MaeII ACGT 4 cut(s) 63, 393, 516, 527
MaeIII GTNAC 1 cut(s) 20
MalI GATC 1 cut(s) 472
MboI GATC 1 cut(s) 470
MboII GAAGA 4 cut(s) 254, 435, 500, 503
MluCI AATT 3 cut(s) 12, 31, 148
MlyI GAGTC 2 cut(s) 230, 641
MnlI CCTC 7 cut(s) 119, 263, 362, 498, 579, 600, 632
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 1 cut(s) 60
MslI CAYNNNNRTG 1 cut(s) 623
MspI CCGG 3 cut(s) 95, 144, 267
MspR9I CCNGG 2 cut(s) 95, 96
MvnI CGCG 1 cut(s) 335
NciI CCSGG 2 cut(s) 95, 96
NdeII GATC 1 cut(s) 470
NlaIII CATG 2 cut(s) 451, 628
NmuCI GTSAC 1 cut(s) 20
PceI AGGCCT 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 75, 301, 329
PinAI ACCGGT 1 cut(s) 143
PleI GAGTC 2 cut(s) 230, 640
PpsI GAGTC 2 cut(s) 230, 640
PpuMI RGGWCCY 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 537
PspPI GGNCC 2 cut(s) 186, 384
PspPPI RGGWCCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 202, 377
RsaNI GTAC 2 cut(s) 201, 376
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 1 cut(s) 60
Sau3AI GATC 1 cut(s) 470
Sau96I GGNCC 2 cut(s) 186, 384
SchI GAGTC 2 cut(s) 230, 641
ScrFI CCNGG 2 cut(s) 95, 96
SetI ASST 8 cut(s) 66, 87, 354, 386, 396, 519, 530, 547
SfaNI GCATC 1 cut(s) 244
SinI GGWCC 1 cut(s) 384
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 3 cut(s) 12, 31, 148
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 2 cut(s) 333, 342
SspMI CTAG 1 cut(s) 569
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 2 cut(s) 93, 94
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 26, 58, 577
TaiI ACGT 4 cut(s) 66, 396, 519, 530
TaqI TCGA 2 cut(s) 197, 510
TasI AATT 3 cut(s) 12, 31, 148
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 3 cut(s) 75, 301, 329
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 486
TseFI GTSAC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 4 cut(s) 99, 300, 607, 666
TspGWI ACGGA 1 cut(s) 500
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 1 cut(s) 486
VpaK11BI GGWCC 1 cut(s) 384
XagI CCTNNNNNAGG 2 cut(s) 123, 594
XmaI CCCGGG 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.