Rroxscaffold_5G00386800

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007081
Physical Location & Seq
Forward (+)
65588409 .. 65590991
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386800.1

Sequence Viewer

Length: 1095 bp
ATGACCTTTGCCGTTTCCGGTTTCGGTAATTTTCCCGCCAATCAGATACGAGCTTTGGCGCCAATTCATAAAGCACCCTATGATCATATCCTTAGGGTAAGAGGCCATTCCTTCGCCGTCGCTAATCCCAAGGGAGCAGCAATGGGTTCCAGAGCTGCCACCAAAAACAAAACCCTGCTGGATATCTTCCAACCCACGACCCGATCCGCCAAACGCTTGAAAACCGATTCTTCCACCCCCAAATCCGATGACGCCATCACCGAGCCTTCTTGTTCTTCTCCGTCAACCCTCACCCCCGAGCAGAAGTCACGCATAGAATTCCAGAAGCTGCTGGCCAAAGCGAAACGCAACCGCACTATCTGCTCCGGCAGAGTCTCGGATTCCAAGGCCAAAGGTGGAGGAGTGAAATTGGAGGAGCTATTGGTGGAGGACACGTGGCTGACGGCGTTGCCTTCTGAGCTTAAGAAGCCCTACGCCGTCAACCTCAGCAAGTTCGTTGAGAGTGAGATCTCCGGCGCCGGCGGTGGTGGTGTTCCGATATATCCGCCGAGTCACTTGATCTTCAATGCTCTGAACTCCACCCCTTTTGATCGGGTCAAGGCTGTCATTTTGGGCCAGGACCCGTATCATGGGCCGGGTCAGGCTATGGGTCTGTCCTTCTCTGTTCCACAAGGAGTGAAGGTCCCATCGAGCCTGGTTAATATATTCAAGGAGCTTCACAAAGATGTTGGATGTTCAATTCCATCTCATGGAAATCTTGAAAAATGGGCTGTTCAGGGTGTTCTGTTGCTCAATGCTGTTCTCACCGTTAGGGATCATCAGGCTAACTCTCATGCAAAGAAAGGATGGGAACAATTTACGGATGCTGTTATCCAAACGATTTCAAAAAAGAAGGAAGGAGTTGTTTTCCTCCTGTGGGGAAACTCTGCACAGCAGAAATCCAGGTTAGTTGATGTGTCGAAGCATCACATTCTTAGAGCGGCACATCCTTCCGGTTTATCTGCATACAGGGGCTTCTTTGGCTGCAGGCATTTTTCTCGCACGAACCAGCTTCTCAAGGAGATGGGTATTCCCCCGATCGATTGGCAACTTTGA

Protein Analysis

364

Amino Acids

39.6

Weight (kDa)

9.84

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDG PF03167 198 - 353 2e-15 Uracil DNA glycosylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 58, 515
AccB7I CCANNNNNTGG 1 cut(s) 749
AccBSI CCGCTC 1 cut(s) 980
AciI CCGC 6 cut(s) 36, 207, 352, 522, 545, 980
AclWI GGATC 2 cut(s) 198, 822
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 1 cut(s) 317
AcvI CACGTG 1 cut(s) 435
AcyI GRCGYC 3 cut(s) 59, 252, 516
AfiI CCNNNNNNNGG 3 cut(s) 629, 749, 916
AflII CTTAAG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 6 cut(s) 220, 565, 709, 738, 761, 885
AjnI CCWGG 3 cut(s) 615, 693, 941
AluBI AGCT 7 cut(s) 53, 155, 328, 418, 460, 715, 1051
AluI AGCT 7 cut(s) 53, 155, 328, 418, 460, 715, 1051
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 2 cut(s) 198, 822
AlwNI CAGNNNCTG 1 cut(s) 328
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 5 cut(s) 103, 333, 387, 613, 632
ApeKI GCWGC 4 cut(s) 137, 155, 328, 1023
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 2 cut(s) 61, 518
AspS9I GGNCC 4 cut(s) 613, 619, 632, 682
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 3 cut(s) 250, 283, 796
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 619, 682
AxyI CCTNAGG 1 cut(s) 92
BalI TGGCCA 1 cut(s) 335
BanI GGYRCC 2 cut(s) 58, 515
BbrPI CACGTG 1 cut(s) 435
BbvCI CCTCAGC 1 cut(s) 485
BbvI GCAGC 4 cut(s) 142, 149, 315, 1010
BccI CCATC 5 cut(s) 263, 694, 751, 840, 1057
BceAI ACGGC 3 cut(s) 101, 459, 461
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 3 cut(s) 617, 695, 943
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 379
BfmI CTRYAG 1 cut(s) 1024
BfoI RGCGCY 2 cut(s) 62, 519
BfrI CTTAAG 1 cut(s) 461
BglII AGATCT 1 cut(s) 507
BisI GCNGC 5 cut(s) 138, 156, 329, 981, 1024
BlsI GCNGC 5 cut(s) 139, 157, 330, 982, 1025
Bme1390I CCNGG 4 cut(s) 617, 636, 695, 943
Bme18I GGWCC 2 cut(s) 619, 682
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 4 cut(s) 613, 619, 632, 682
BmiI GGNNCC 5 cut(s) 60, 148, 517, 621, 684
BmrFI CCNGG 4 cut(s) 617, 636, 695, 943
BmsI GCATC 2 cut(s) 853, 973
Bpu10I CCTNAGC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 1040
BpuMI CCSGG 1 cut(s) 636
Bsa29I ATCGAT 1 cut(s) 1080
BsaAI YACGTR 1 cut(s) 435
BsaHI GRCGYC 3 cut(s) 59, 252, 516
BsaJI CCNNGG 2 cut(s) 129, 384
BsaWI WCCGGW 2 cut(s) 17, 992
BsaXI ACNNNNNCTCC 4 cut(s) 419, 449, 666, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 629, 749, 916
Bse118I RCCGGY 1 cut(s) 518
Bse21I CCTNAGG 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 147
BseBI CCWGG 3 cut(s) 617, 695, 943
BseCI ATCGAT 1 cut(s) 1080
BseDI CCNNGG 2 cut(s) 129, 384
BseGI GGATG 4 cut(s) 737, 851, 868, 985
BseLI CCNNNNNNNGG 3 cut(s) 629, 749, 916
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 447, 499
BseRI GAGGAG 2 cut(s) 414, 428
BseXI GCAGC 4 cut(s) 142, 149, 315, 1010
BsgI GTGCAG 1 cut(s) 912
Bsh1285I CGRYCG 1 cut(s) 1080
BshFI GGCC 5 cut(s) 105, 335, 389, 615, 634
BshNI GGYRCC 2 cut(s) 58, 515
BshVI ATCGAT 1 cut(s) 1080
BsiEI CGRYCG 1 cut(s) 1080
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 6 cut(s) 18, 366, 513, 519, 635, 993
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 3 cut(s) 629, 749, 916
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 5 cut(s) 105, 335, 389, 615, 634
BsoBI CYCGRG 1 cut(s) 296
Bsp143I GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
BspACI CCGC 6 cut(s) 36, 207, 352, 522, 545, 980
BspANI GGCC 5 cut(s) 105, 335, 389, 615, 634
BspCNI CTCAG 2 cut(s) 448, 498
BspDI ATCGAT 1 cut(s) 1080
BspLI GGNNCC 5 cut(s) 60, 148, 517, 621, 684
BspMAI CTGCAG 1 cut(s) 1028
BspPI GGATC 2 cut(s) 198, 822
BspT107I GGYRCC 2 cut(s) 58, 515
BspTI CTTAAG 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 129, 384
BssMI GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
BssNI GRCGYC 3 cut(s) 59, 252, 516
BssT1I CCWWGG 2 cut(s) 129, 384
Bst2UI CCWGG 3 cut(s) 617, 695, 943
Bst4CI ACNGT 1 cut(s) 808
BstACI GRCGYC 3 cut(s) 59, 252, 516
BstAFI CTTAAG 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 333, 520, 1028
BstDEI CTNAG 4 cut(s) 92, 456, 485, 974
BstF5I GGATG 4 cut(s) 737, 851, 868, 985
BstH2I RGCGCY 2 cut(s) 62, 519
BstHHI GCGC 2 cut(s) 61, 518
BstKTI GATC 7 cut(s) 85, 206, 510, 561, 592, 817, 1080
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
BstMCI CGRYCG 1 cut(s) 1080
BstMWI GCNNNNNNNGC 4 cut(s) 360, 457, 466, 1020
BstNI CCWGG 3 cut(s) 617, 695, 943
BstSCI CCNGG 4 cut(s) 615, 634, 693, 941
BstSFI CTRYAG 1 cut(s) 1024
BstV1I GCAGC 4 cut(s) 142, 149, 315, 1010
BstX2I RGATCY 1 cut(s) 507
BstYI RGATCY 1 cut(s) 507
Bsu15I ATCGAT 1 cut(s) 1080
Bsu36I CCTNAGG 1 cut(s) 92
BsuRI GGCC 5 cut(s) 105, 335, 389, 615, 634
BsuTUI ATCGAT 1 cut(s) 1080
BtsCI GGATG 4 cut(s) 737, 851, 868, 985
Cac8I GCNNGC 3 cut(s) 333, 520, 1028
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 2 cut(s) 61, 518
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 4 cut(s) 613, 619, 632, 682
ClaI ATCGAT 1 cut(s) 1080
CseI GACGC 1 cut(s) 260
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 3 cut(s) 629, 749, 833
DdeI CTNAG 4 cut(s) 92, 456, 485, 974
DinI GGCGCC 2 cut(s) 60, 517
DpnI GATC 7 cut(s) 84, 205, 509, 560, 591, 816, 1079
DpnII GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
EaeI YGGCCR 1 cut(s) 333
EciI GGCGGA 2 cut(s) 196, 534
Eco130I CCWWGG 2 cut(s) 129, 384
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 619, 682
Eco72I CACGTG 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 296
EcoO109I RGGNCCY 2 cut(s) 619, 682
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 615, 693, 941
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 129, 384
EgeI GGCGCC 2 cut(s) 60, 517
EheI GGCGCC 2 cut(s) 60, 517
ErhI CCWWGG 2 cut(s) 129, 384
FaeI CATG 3 cut(s) 632, 752, 836
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 628, 748, 832
FauI CCCGC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 5 cut(s) 138, 156, 329, 981, 1024
FokI GGATG 4 cut(s) 744, 858, 875, 972
Fsp4HI GCNGC 5 cut(s) 138, 156, 329, 981, 1024
GlaI GCGC 2 cut(s) 60, 517
GluI GCNGC 5 cut(s) 138, 156, 329, 981, 1024
HaeII RGCGCY 2 cut(s) 62, 519
HaeIII GGCC 5 cut(s) 105, 335, 389, 615, 634
HapII CCGG 6 cut(s) 18, 366, 513, 519, 635, 993
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 61, 518
Hin1I GRCGYC 3 cut(s) 59, 252, 516
Hin1II CATG 3 cut(s) 632, 752, 836
Hin6I GCGC 2 cut(s) 59, 516
HinP1I GCGC 2 cut(s) 59, 516
HincII GTYRAC 2 cut(s) 285, 481
HindII GTYRAC 2 cut(s) 285, 481
HinfI GANTC 4 cut(s) 227, 372, 380, 550
HpaII CCGG 6 cut(s) 18, 366, 513, 519, 635, 993
HphI GGTGA 3 cut(s) 250, 283, 796
Hpy166II GTNNAC 2 cut(s) 285, 481
Hpy188I TCNGA 6 cut(s) 45, 247, 379, 457, 537, 573
Hpy188III TCNNGA 3 cut(s) 150, 322, 758
Hpy8I GTNNAC 2 cut(s) 285, 481
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 8 cut(s) 121, 276, 462, 667, 673, 886, 890, 999
HpyCH4III ACNGT 1 cut(s) 808
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 4 cut(s) 836, 929, 1004, 1026
HpyF10VI GCNNNNNNNGC 4 cut(s) 360, 457, 466, 1020
HpyF3I CTNAG 4 cut(s) 92, 456, 485, 974
HpySE526I ACGT 1 cut(s) 434
Hsp92I GRCGYC 3 cut(s) 59, 252, 516
Hsp92II CATG 3 cut(s) 632, 752, 836
HspAI GCGC 2 cut(s) 59, 516
KasI GGCGCC 2 cut(s) 58, 515
KroI GCCGGC 1 cut(s) 518
KroNI GCCGGC 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
LmnI GCTCC 4 cut(s) 134, 368, 415, 712
Lsp1109I GCAGC 4 cut(s) 142, 149, 315, 1010
LweI GCATC 2 cut(s) 853, 973
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 2 cut(s) 306, 551
MalI GATC 7 cut(s) 84, 205, 509, 560, 591, 816, 1079
MbiI CCGCTC 1 cut(s) 980
MboI GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
MboII GAAGA 4 cut(s) 178, 222, 267, 553
MflI RGATCY 1 cut(s) 507
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 6 cut(s) 28, 63, 317, 407, 738, 854
MluNI TGGCCA 1 cut(s) 335
Mly113I GGCGCC 2 cut(s) 59, 516
MlyI GAGTC 2 cut(s) 381, 559
MmeI TCCRAC 2 cut(s) 214, 709
MnlI CCTC 7 cut(s) 95, 299, 392, 406, 421, 494, 920
Mox20I TGGCCA 1 cut(s) 335
MreI CGCCGGCG 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 518
MscI TGGCCA 1 cut(s) 335
MseI TTAA 2 cut(s) 462, 699
MslI CAYNNNNRTG 1 cut(s) 723
Msp20I TGGCCA 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 6 cut(s) 18, 366, 513, 519, 635, 993
MspR9I CCNGG 4 cut(s) 617, 636, 695, 943
MvaI CCWGG 3 cut(s) 617, 695, 943
MwoI GCNNNNNNNGC 4 cut(s) 360, 457, 466, 1020
NaeI GCCGGC 1 cut(s) 520
NarI GGCGCC 2 cut(s) 59, 516
NciI CCSGG 1 cut(s) 636
NdeII GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
NgoMIV GCCGGC 1 cut(s) 518
NlaIII CATG 3 cut(s) 632, 752, 836
NlaIV GGNNCC 5 cut(s) 60, 148, 517, 621, 684
NmeAIII GCCGAG 1 cut(s) 573
NmuCI GTSAC 2 cut(s) 306, 551
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 227, 380
PflMI CCANNNNNTGG 1 cut(s) 749
PkrI GCNGC 5 cut(s) 139, 157, 330, 982, 1025
Ple19I CGATCG 1 cut(s) 1080
PleI GAGTC 2 cut(s) 380, 558
PluTI GGCGCC 2 cut(s) 62, 519
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
PpsI GAGTC 2 cut(s) 380, 558
Ppu21I YACGTR 1 cut(s) 435
PpuMI RGGWCCY 2 cut(s) 619, 682
Psp5II RGGWCCY 2 cut(s) 619, 682
Psp6I CCWGG 3 cut(s) 615, 693, 941
PspCI CACGTG 1 cut(s) 435
PspGI CCWGG 3 cut(s) 615, 693, 941
PspN4I GGNNCC 5 cut(s) 60, 148, 517, 621, 684
PspPI GGNCC 4 cut(s) 613, 619, 632, 682
PspPPI RGGWCCY 2 cut(s) 619, 682
PstI CTGCAG 1 cut(s) 1028
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 507
PvuI CGATCG 1 cut(s) 1080
RseI CAYNNNNRTG 1 cut(s) 723
SaqAI TTAA 2 cut(s) 462, 699
SatI GCNGC 5 cut(s) 138, 156, 329, 981, 1024
Sau3AI GATC 7 cut(s) 82, 203, 507, 558, 589, 814, 1077
Sau96I GGNCC 4 cut(s) 613, 619, 632, 682
SchI GAGTC 2 cut(s) 381, 559
ScrFI CCNGG 4 cut(s) 617, 636, 695, 943
SfaNI GCATC 2 cut(s) 853, 973
SfcI CTRYAG 1 cut(s) 1024
SfoI GGCGCC 2 cut(s) 60, 517
SgrAI CRCCGGYG 1 cut(s) 518
SinI GGWCC 2 cut(s) 619, 682
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 2 cut(s) 461, 1055
SmoI CTYRAG 2 cut(s) 461, 1055
Sse9I AATT 6 cut(s) 28, 63, 317, 407, 738, 854
SsiI CCGC 6 cut(s) 36, 207, 352, 522, 545, 980
SspDI GGCGCC 2 cut(s) 58, 515
StyD4I CCNGG 4 cut(s) 615, 634, 693, 941
StyI CCWWGG 2 cut(s) 129, 384
TaaI ACNGT 1 cut(s) 808
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 689, 959, 1080
TasI AATT 6 cut(s) 28, 63, 317, 407, 738, 854
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 2 cut(s) 227, 380
Tru1I TTAA 2 cut(s) 462, 699
Tru9I TTAA 2 cut(s) 462, 699
TseFI GTSAC 2 cut(s) 306, 551
TseI GCWGC 4 cut(s) 137, 155, 328, 1023
Tsp45I GTSAC 2 cut(s) 306, 551
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 2 cut(s) 270, 875
Van91I CCANNNNNTGG 1 cut(s) 749
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 619, 682
XapI RAATTY 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.