RchiOBHm_Chr4g0446361

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007081
Physical Location & Seq
Forward (+)
66563021 .. 66565432
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41388

Sequence Viewer

Length: 1092 bp
ATGACCTTTGCCGTTTCCGGTTTCGGTAATTTTCCCGCCAATCAGATACGAGCTTTGGCGCCAATTCACAAAGCAGCCTATGATCATATCATTAGGGTAAGAGGCCATTCCTTCGCCGTCGCTAATCCCAAGGGAGCAGCAATGGGTTCCAGAGCTGCCACCAAAAACAAAACCTTACTGGATATCTTCCAACCCACGACCCGATCCGCCAAACGCTTCAAAACCGATTCTTCCACCCCCAAATCCGATGACGCCATCACCGAGCCTTCTTCTTCTCCGTCAGCCCTCACCCCCGAGCAGAAGTCACGCATAGAATTCCAGAAGCTGCTGGCCAAAGCGAAACGCAACCGCACTATCTGCTCCGGCAGAGTCTCCGATTCCAAGGCCAAAGGTGAAGGAGTGAAATTGGAGGAGCTATTGGTGGAGGACACGTGGCTGACGGCGTTGCCTTCTGAGCTTAAGAAGCCCTACGCCGTCAACCTCAGCAAGTTCGTTGAGAGTGAGATTTCCGGCGCCGGCGGTGGTGGTGTTCCGATATATCCGCCGAGTCACTTGATCTTCAATGCTCTGAACTCCACCCCTTTTGATCGGGTCAAGGCTGTCATTTTGGGCCAGGACCCGTATCATGGGCCGGGTCAGGCTATGGGTCTGTCCTTCTCTGTTCCCCAAGGAGTGAAGGTCCCATCGAGCCTGGTTAATATATTCAAGGAGCTTCACAAAGACGTTGGCTGTTCAATTCCATCTCATGGAAATCTAGAAAAATGGGCTGTTCAGGGTGTTCTGTTGCTCAATGCTGTTCTCACCGTTAGGGATCATCAGGCTAACTCTCATGCAAAGAAAGGATGGGAACAATTTACGGATGCTGTTATCCAAACGATTTCAAAAAAGAAGGAAGGAGTTGTTTTCCTCCTGTGGGGAAACTCCGCACAGCAGAAATCCAGGTTAGTTGATGTGTCGAAGCATCACATTCTTAGAGCGGCACATCCTTCCGGTTTATCTGCATACAGGGGCTTCTTTGGCTGCAGGCATTTTTCTCGCACGAACCAGCTTCTCAAGGAGATGGGTATTCCCCCGATCGATTGGCAACTTTGA

Protein Analysis

363

Amino Acids

39.55

Weight (kDa)

9.84

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDG PF03167 197 - 352 2e-15 Uracil DNA glycosylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 58, 512
AccB7I CCANNNNNTGG 1 cut(s) 746
AccBSI CCGCTC 1 cut(s) 977
AciI CCGC 7 cut(s) 36, 207, 349, 519, 542, 924, 977
AclWI GGATC 2 cut(s) 198, 819
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 314
AcvI CACGTG 1 cut(s) 432
AcyI GRCGYC 3 cut(s) 59, 252, 513
AfiI CCNNNNNNNGG 3 cut(s) 626, 746, 913
AflII CTTAAG 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 5 cut(s) 220, 562, 706, 735, 882
AjnI CCWGG 3 cut(s) 612, 690, 938
AluBI AGCT 7 cut(s) 53, 155, 325, 415, 457, 712, 1048
AluI AGCT 7 cut(s) 53, 155, 325, 415, 457, 712, 1048
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 2 cut(s) 198, 819
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 5 cut(s) 103, 330, 384, 610, 629
ApeKI GCWGC 5 cut(s) 74, 137, 155, 325, 1020
ApoI RAATTY 1 cut(s) 314
AspLEI GCGC 2 cut(s) 61, 515
AspS9I GGNCC 4 cut(s) 610, 616, 629, 679
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 4 cut(s) 250, 280, 404, 793
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 2 cut(s) 616, 679
BalI TGGCCA 1 cut(s) 332
BanI GGYRCC 2 cut(s) 58, 512
BbrPI CACGTG 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 5 cut(s) 86, 142, 149, 312, 1007
BccI CCATC 5 cut(s) 263, 691, 748, 837, 1054
BceAI ACGGC 3 cut(s) 101, 456, 458
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciT130I CCWGG 3 cut(s) 614, 692, 940
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 1 cut(s) 1021
BfoI RGCGCY 2 cut(s) 62, 516
BfrI CTTAAG 1 cut(s) 458
BisI GCNGC 6 cut(s) 75, 138, 156, 326, 978, 1021
BlsI GCNGC 6 cut(s) 76, 139, 157, 327, 979, 1022
Bme1390I CCNGG 4 cut(s) 614, 633, 692, 940
Bme18I GGWCC 2 cut(s) 616, 679
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 4 cut(s) 610, 616, 629, 679
BmiI GGNNCC 5 cut(s) 60, 148, 514, 618, 681
BmrFI CCNGG 4 cut(s) 614, 633, 692, 940
BmsI GCATC 2 cut(s) 850, 970
Bpu10I CCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 1077
BsaAI YACGTR 1 cut(s) 432
BsaHI GRCGYC 3 cut(s) 59, 252, 513
BsaJI CCNNGG 3 cut(s) 129, 381, 667
BsaWI WCCGGW 2 cut(s) 17, 989
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 663, 693
Bsc4I CCNNNNNNNGG 3 cut(s) 626, 746, 913
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 147
BseBI CCWGG 3 cut(s) 614, 692, 940
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 3 cut(s) 129, 381, 667
BseGI GGATG 3 cut(s) 848, 865, 982
BseLI CCNNNNNNNGG 3 cut(s) 626, 746, 913
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 444, 496
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 5 cut(s) 86, 142, 149, 312, 1007
Bsh1285I CGRYCG 1 cut(s) 1077
BshFI GGCC 5 cut(s) 105, 332, 386, 612, 631
BshNI GGYRCC 2 cut(s) 58, 512
BshVI ATCGAT 1 cut(s) 1077
BsiEI CGRYCG 1 cut(s) 1077
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 6 cut(s) 18, 363, 510, 516, 632, 990
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 626, 746, 913
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 5 cut(s) 105, 332, 386, 612, 631
BsoBI CYCGRG 1 cut(s) 293
Bsp143I GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
BspACI CCGC 7 cut(s) 36, 207, 349, 519, 542, 924, 977
BspANI GGCC 5 cut(s) 105, 332, 386, 612, 631
BspCNI CTCAG 2 cut(s) 445, 495
BspDI ATCGAT 1 cut(s) 1077
BspLI GGNNCC 5 cut(s) 60, 148, 514, 618, 681
BspMAI CTGCAG 1 cut(s) 1025
BspPI GGATC 2 cut(s) 198, 819
BspT107I GGYRCC 2 cut(s) 58, 512
BspTI CTTAAG 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 977
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 1 cut(s) 183
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 3 cut(s) 129, 381, 667
BssMI GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
BssNI GRCGYC 3 cut(s) 59, 252, 513
BssT1I CCWWGG 3 cut(s) 129, 381, 667
Bst2UI CCWGG 3 cut(s) 614, 692, 940
Bst4CI ACNGT 1 cut(s) 805
BstACI GRCGYC 3 cut(s) 59, 252, 513
BstAFI CTTAAG 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 432
BstC8I GCNNGC 3 cut(s) 330, 517, 1025
BstDEI CTNAG 3 cut(s) 453, 482, 971
BstF5I GGATG 3 cut(s) 848, 865, 982
BstH2I RGCGCY 2 cut(s) 62, 516
BstHHI GCGC 2 cut(s) 61, 515
BstKTI GATC 6 cut(s) 85, 206, 558, 589, 814, 1077
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
BstMCI CGRYCG 1 cut(s) 1077
BstMWI GCNNNNNNNGC 4 cut(s) 357, 454, 463, 1017
BstNI CCWGG 3 cut(s) 614, 692, 940
BstSCI CCNGG 4 cut(s) 612, 631, 690, 938
BstSFI CTRYAG 1 cut(s) 1021
BstV1I GCAGC 5 cut(s) 86, 142, 149, 312, 1007
Bsu15I ATCGAT 1 cut(s) 1077
BsuRI GGCC 5 cut(s) 105, 332, 386, 612, 631
BsuTUI ATCGAT 1 cut(s) 1077
BtsCI GGATG 3 cut(s) 848, 865, 982
Cac8I GCNNGC 3 cut(s) 330, 517, 1025
CaiI CAGNNNCTG 1 cut(s) 325
CfoI GCGC 2 cut(s) 61, 515
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 4 cut(s) 610, 616, 629, 679
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 1 cut(s) 260
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 3 cut(s) 626, 746, 830
DdeI CTNAG 3 cut(s) 453, 482, 971
DinI GGCGCC 2 cut(s) 60, 514
DpnI GATC 6 cut(s) 84, 205, 557, 588, 813, 1076
DpnII GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
EaeI YGGCCR 1 cut(s) 330
EciI GGCGGA 2 cut(s) 196, 531
Eco130I CCWWGG 3 cut(s) 129, 381, 667
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 616, 679
Eco72I CACGTG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 293
EcoO109I RGGNCCY 2 cut(s) 616, 679
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 612, 690, 938
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 3 cut(s) 129, 381, 667
EgeI GGCGCC 2 cut(s) 60, 514
EheI GGCGCC 2 cut(s) 60, 514
ErhI CCWWGG 3 cut(s) 129, 381, 667
FaeI CATG 3 cut(s) 629, 749, 833
FaqI GGGAC 1 cut(s) 665
FatI CATG 3 cut(s) 625, 745, 829
FauI CCCGC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 6 cut(s) 75, 138, 156, 326, 978, 1021
FokI GGATG 3 cut(s) 855, 872, 969
Fsp4HI GCNGC 6 cut(s) 75, 138, 156, 326, 978, 1021
FspBI CTAG 1 cut(s) 755
GlaI GCGC 2 cut(s) 60, 514
GluI GCNGC 6 cut(s) 75, 138, 156, 326, 978, 1021
HaeII RGCGCY 2 cut(s) 62, 516
HaeIII GGCC 5 cut(s) 105, 332, 386, 612, 631
HapII CCGG 6 cut(s) 18, 363, 510, 516, 632, 990
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 61, 515
Hin1I GRCGYC 3 cut(s) 59, 252, 513
Hin1II CATG 3 cut(s) 629, 749, 833
Hin6I GCGC 2 cut(s) 59, 513
HinP1I GCGC 2 cut(s) 59, 513
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 4 cut(s) 227, 369, 377, 547
HpaII CCGG 6 cut(s) 18, 363, 510, 516, 632, 990
HphI GGTGA 4 cut(s) 250, 280, 404, 793
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 6 cut(s) 45, 247, 376, 454, 534, 570
Hpy188III TCNNGA 3 cut(s) 150, 319, 755
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 9 cut(s) 121, 276, 389, 459, 664, 670, 883, 887, 996
HpyCH4III ACNGT 1 cut(s) 805
HpyCH4IV ACGT 2 cut(s) 431, 723
HpyCH4V TGCA 3 cut(s) 833, 1001, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 357, 454, 463, 1017
HpyF3I CTNAG 3 cut(s) 453, 482, 971
HpySE526I ACGT 2 cut(s) 431, 723
Hsp92I GRCGYC 3 cut(s) 59, 252, 513
Hsp92II CATG 3 cut(s) 629, 749, 833
HspAI GCGC 2 cut(s) 59, 513
KasI GGCGCC 2 cut(s) 58, 512
KroI GCCGGC 1 cut(s) 515
KroNI GCCGGC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
LmnI GCTCC 4 cut(s) 134, 365, 412, 709
Lsp1109I GCAGC 5 cut(s) 86, 142, 149, 312, 1007
LweI GCATC 2 cut(s) 850, 970
MaeI CTAG 1 cut(s) 755
MaeII ACGT 2 cut(s) 431, 723
MaeIII GTNAC 2 cut(s) 303, 548
MalI GATC 6 cut(s) 84, 205, 557, 588, 813, 1076
MbiI CCGCTC 1 cut(s) 977
MboI GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
MboII GAAGA 5 cut(s) 178, 222, 261, 264, 550
MlsI TGGCCA 1 cut(s) 332
MluCI AATT 6 cut(s) 28, 63, 314, 404, 735, 851
MluNI TGGCCA 1 cut(s) 332
Mly113I GGCGCC 2 cut(s) 59, 513
MlyI GAGTC 2 cut(s) 378, 556
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 95, 296, 403, 418, 491, 917
Mox20I TGGCCA 1 cut(s) 332
MreI CGCCGGCG 1 cut(s) 515
MroNI GCCGGC 1 cut(s) 515
MscI TGGCCA 1 cut(s) 332
MseI TTAA 2 cut(s) 459, 696
Msp20I TGGCCA 1 cut(s) 332
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 6 cut(s) 18, 363, 510, 516, 632, 990
MspR9I CCNGG 4 cut(s) 614, 633, 692, 940
MvaI CCWGG 3 cut(s) 614, 692, 940
MwoI GCNNNNNNNGC 4 cut(s) 357, 454, 463, 1017
NaeI GCCGGC 1 cut(s) 517
NarI GGCGCC 2 cut(s) 59, 513
NciI CCSGG 1 cut(s) 633
NdeII GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
NgoMIV GCCGGC 1 cut(s) 515
NlaIII CATG 3 cut(s) 629, 749, 833
NlaIV GGNNCC 5 cut(s) 60, 148, 514, 618, 681
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 303, 548
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 227, 377
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 6 cut(s) 76, 139, 157, 327, 979, 1022
Ple19I CGATCG 1 cut(s) 1077
PleI GAGTC 2 cut(s) 377, 555
PluTI GGCGCC 2 cut(s) 62, 516
PmaCI CACGTG 1 cut(s) 432
PmlI CACGTG 1 cut(s) 432
PpsI GAGTC 2 cut(s) 377, 555
Ppu21I YACGTR 1 cut(s) 432
PpuMI RGGWCCY 2 cut(s) 616, 679
Psp5II RGGWCCY 2 cut(s) 616, 679
Psp6I CCWGG 3 cut(s) 612, 690, 938
PspCI CACGTG 1 cut(s) 432
PspGI CCWGG 3 cut(s) 612, 690, 938
PspN4I GGNNCC 5 cut(s) 60, 148, 514, 618, 681
PspPI GGNCC 4 cut(s) 610, 616, 629, 679
PspPPI RGGWCCY 2 cut(s) 616, 679
PstI CTGCAG 1 cut(s) 1025
PstNI CAGNNNCTG 1 cut(s) 325
PvuI CGATCG 1 cut(s) 1077
SaqAI TTAA 2 cut(s) 459, 696
SatI GCNGC 6 cut(s) 75, 138, 156, 326, 978, 1021
Sau3AI GATC 6 cut(s) 82, 203, 555, 586, 811, 1074
Sau96I GGNCC 4 cut(s) 610, 616, 629, 679
SchI GAGTC 2 cut(s) 378, 556
ScrFI CCNGG 4 cut(s) 614, 633, 692, 940
SfaNI GCATC 2 cut(s) 850, 970
SfcI CTRYAG 1 cut(s) 1021
SfoI GGCGCC 2 cut(s) 60, 514
SgrAI CRCCGGYG 1 cut(s) 515
SinI GGWCC 2 cut(s) 616, 679
SmlI CTYRAG 2 cut(s) 458, 1052
SmoI CTYRAG 2 cut(s) 458, 1052
Sse9I AATT 6 cut(s) 28, 63, 314, 404, 735, 851
SsiI CCGC 7 cut(s) 36, 207, 349, 519, 542, 924, 977
SspDI GGCGCC 2 cut(s) 58, 512
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 4 cut(s) 612, 631, 690, 938
StyI CCWWGG 3 cut(s) 129, 381, 667
TaaI ACNGT 1 cut(s) 805
TaiI ACGT 2 cut(s) 434, 726
TaqI TCGA 3 cut(s) 686, 956, 1077
TasI AATT 6 cut(s) 28, 63, 314, 404, 735, 851
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 2 cut(s) 227, 377
Tru1I TTAA 2 cut(s) 459, 696
Tru9I TTAA 2 cut(s) 459, 696
TseFI GTSAC 2 cut(s) 303, 548
TseI GCWGC 5 cut(s) 74, 137, 155, 325, 1020
Tsp45I GTSAC 2 cut(s) 303, 548
TspGWI ACGGA 2 cut(s) 267, 872
Van91I CCANNNNNTGG 1 cut(s) 746
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 616, 679
XapI RAATTY 1 cut(s) 314
XbaI TCTAGA 1 cut(s) 754
XspI CTAG 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.