Rorug04G0370700

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007081
Physical Location & Seq
Reverse (-)
54177281 .. 54178184
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0370700.1

Sequence Viewer

Length: 459 bp
ATGGACAACGGCGCGTCGTCTCCGGTTCCCAGAATAGGTGTGGTGGTGTTTCTTCTCAGAGGGAAAACTGTGCTTCTAGGACGACGTCGTTCCTCCATCGGCGACTCCACTTTTGCTCTCCCCGGTGGCCACCTCGAGTTTGGTGAGGGTTTTGAGGAGTGTGCGGCGAGAGAATTGAAGGAAGAGACGGGTCTGGACCTCGACAAGATCGAATTTGTGACCGTGACGAACAACTTGTTCTTGGACGAACCCAAACCGGCGCATTATGTGACGATCTTTATGCGAGCGGTTCTGAAAGATCCCGATCAAGTTCCCCAAACTGTTGAGCCGGACAAGTGTGACGGTTGGGGTTGGTACGAGTGGGACGACCTCCCCAAACCACTGTTTTGGCCTTTGGAGAAGATGGTGCAGAGTGGGTTTAGTCCTTTTCCAATTCCGGATTCCAATTCAGGGCAGTAA

Protein Analysis

152

Amino Acids

16.98

Weight (kDa)

4.65

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 10 - 135 3.7e-22 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 88
AccBSI CCGCTC 1 cut(s) 287
AccII CGCG 1 cut(s) 14
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 2 cut(s) 164, 287
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 2 cut(s) 35, 450
AgsI TTSAA 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 424, 456
Alw26I GTCTC 2 cut(s) 24, 179
AlwI GGATC 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 2 cut(s) 127, 389
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 2 cut(s) 14, 262
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 129
BccI CCATC 2 cut(s) 104, 397
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 24, 179
BfaI CTAG 1 cut(s) 77
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 121
BsaWI WCCGGW 2 cut(s) 22, 436
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 450
Bse118I RCCGGY 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 303
BseAI TCCGGA 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 35, 450
BseMII CTCAG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 2 cut(s) 129, 391
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 5 cut(s) 23, 123, 257, 329, 437
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 35, 450
BsmAI GTCTC 2 cut(s) 24, 179
BsmBI CGTCTC 2 cut(s) 24, 179
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 129, 391
BsoBI CYCGRG 1 cut(s) 134
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 4 cut(s) 207, 273, 298, 304
BspACI CCGC 2 cut(s) 164, 287
BspANI GGCC 2 cut(s) 129, 391
BspCNI CTCAG 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 4 cut(s) 207, 273, 298, 304
BssNI GRCGYC 1 cut(s) 85
Bst4CI ACNGT 5 cut(s) 70, 223, 322, 344, 384
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 56
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 2 cut(s) 14, 262
BstKTI GATC 4 cut(s) 210, 276, 301, 307
BstMAI GTCTC 2 cut(s) 24, 179
BstMBI GATC 4 cut(s) 207, 273, 298, 304
BstSCI CCNGG 1 cut(s) 121
BstUI CGCG 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 387
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 2 cut(s) 129, 391
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 285
CfoI GCGC 2 cut(s) 14, 262
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 3
Csp6I GTAC 1 cut(s) 355
CviJI RGCY 3 cut(s) 129, 328, 391
CviKI_1 RGCY 3 cut(s) 129, 328, 391
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 1 cut(s) 56
DpnI GATC 4 cut(s) 209, 275, 300, 306
DpnII GATC 4 cut(s) 207, 273, 298, 304
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 134
Esp3I CGTCTC 2 cut(s) 24, 179
FaiI YATR 2 cut(s) 267, 281
FaqI GGGAC 1 cut(s) 377
Fnu4HI GCNGC 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 1 cut(s) 77
GlaI GCGC 2 cut(s) 13, 261
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 129, 391
HapII CCGG 5 cut(s) 23, 123, 257, 329, 437
HgaI GACGC 1 cut(s) 3
HhaI GCGC 2 cut(s) 14, 262
Hin1I GRCGYC 1 cut(s) 85
Hin6I GCGC 2 cut(s) 12, 260
HinP1I GCGC 2 cut(s) 12, 260
HinfI GANTC 2 cut(s) 104, 440
HpaII CCGG 5 cut(s) 23, 123, 257, 329, 437
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 59, 294
Hpy188III TCNNGA 3 cut(s) 194, 302, 437
Hpy99I CGWCG 3 cut(s) 19, 87, 90
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 5 cut(s) 70, 223, 322, 344, 384
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 1 cut(s) 409
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 85
Hsp92I GRCGYC 1 cut(s) 85
HspAI GCGC 2 cut(s) 12, 260
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 4 cut(s) 207, 273, 298, 304
LpnPI CCDG 8 cut(s) 36, 43, 136, 179, 270, 342, 435, 450
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 4 cut(s) 217, 223, 268, 338
MalI GATC 4 cut(s) 209, 275, 300, 306
MbiI CCGCTC 1 cut(s) 287
MboI GATC 4 cut(s) 207, 273, 298, 304
MboII GAAGA 3 cut(s) 44, 194, 412
MflI RGATCY 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 4 cut(s) 173, 212, 432, 445
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 7 cut(s) 53, 103, 139, 143, 148, 209, 380
Mox20I TGGCCA 1 cut(s) 129
MroI TCCGGA 1 cut(s) 436
MscI TGGCCA 1 cut(s) 129
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 5 cut(s) 23, 123, 257, 329, 437
MspR9I CCNGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 14
NciI CCSGG 1 cut(s) 123
NdeII GATC 4 cut(s) 207, 273, 298, 304
NlaIV GGNNCC 1 cut(s) 27
NmuCI GTSAC 4 cut(s) 217, 223, 268, 338
PaeR7I CTCGAG 1 cut(s) 134
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 363
PfeI GAWTC 1 cut(s) 440
PflFI GACNNNGTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 196
PspXI VCTCGAGB 1 cut(s) 134
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 84
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 4 cut(s) 207, 273, 298, 304
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 123
SetI ASST 5 cut(s) 40, 88, 135, 201, 372
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 4 cut(s) 173, 212, 432, 445
SsiI CCGC 2 cut(s) 164, 287
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 5 cut(s) 70, 223, 322, 344, 384
TaiI ACGT 1 cut(s) 88
TaqI TCGA 3 cut(s) 135, 201, 210
TasI AATT 4 cut(s) 173, 212, 432, 445
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 440
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 4 cut(s) 217, 223, 268, 338
Tsp45I GTSAC 4 cut(s) 217, 223, 268, 338
TspRI CASTG 1 cut(s) 387
Tth111I GACNNNGTC 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 2 cut(s) 37, 137
XhoI CTCGAG 1 cut(s) 134
XspI CTAG 1 cut(s) 77
ZraI GACGTC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.