Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002013

Physical Location & Seq

Genomic Coordinates Forward (+)

5871310 .. 5880452

Gene Structure 9143 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1083700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4251 bp

ATGAAGAGGTCCAGGGACGAAGTTTTTATGAGCTCGCAACTCAAGCGGCCCGTGGTTTCCGCTCGAGGAGAGCCTTCTGGGCAACCCCAGATGATGGCAGCAGCAGCAGCAGCTTCCCAAAAGCTAACAACAAATGATGCCTTAGCTTATCTAAAAGCGGTGAAGGATATATTTCAAGACAAAAACAGGGGAAAATACGAAGAATTTCTTGAAGTCATGAAAGACTTCAAGGCCACAAGAATTGACACTGCGGGTGTGATAGAGAGGGTGAAAGATTTATTTAAAGGGCATCGAGAGCTAATATTAGGTTTCAATACCTTCTTGCCAAAGGGATATGAAATAACCCTCCCACTTGATGATGACCAACAACCTCCTCAAAAGAAGCCTGTTGAATTTGAGGAAGCCATCAATTTCGTGAACAAGATTAAGACACGATTTCAAGGCGACGACCATGTTTATAAATCTTTTTTAGACATTCTGAATATGTACAGAAAGGAAAACAAGTCCATCCAAGAGGTTTACCAGGAGGTTGCTGCACTTTTCCAAGACCACCCAGATTTGCTTGTGGAGTTTACCCACTTTTTACCTGATACTACAGGAACGGCTTCCATTCATCCACCTCACAGAAACTCAATGCTTCGGGATAGGAGCTCTGCAATGCCTACTATGCGGCAGATGCATGCAGACAAGGAAAGGACCGTGGGTTCTTATGCTGACCATGATCTCAGTGTTGACCGTCCTGATCCTGACCATGATAAAGCTCTGATGAAAGCAGACAAAGAGCAAAGGAGGCGTGGCGAGAAGGAGAAGGAGAGGAGAGAAGACAGAGAAAGAAGAGAACGGGATGATAGGGATTTTGACCATGATGGCAGTAGAGACCTCAATATGCAGCGTTTTCCCCACAAACAGAAATCTGCTCGTAGGACTGAAGACACTGAACAATTGCAGCCAGGTATGTACGGCCAGGAGTTTGCTTTCTGCGAGAAGGTGAAGGAGAAGTTACGAAATCCTGAAGATTACCAGGAATTTTTGAAGTGCCTTCATATTTATAGCAAGGAAATTATTACACGCTCTGAATTGCAGTCTTTGGTGGCTGATTTAATTGGAAGATATCCTGAACTTATGGATGGGTTCGATGATTTTTTGGCATGTTGTGAGAAGAAAGATGGGTTCCTTGCTGGTGTCATGAGTAAAAAATCCTTGTGGAATGAAGGACATTTACCCAGATCAGTGAAGGTAGAAGATAGGGATAGGGATAGAGATCGTGAAAGGGATGATGGGGTTAAAGATAGAGAGCATGAAACTCGAGAAAGAGATAGACTTGATAAAAATGGTGCCTTTGGAATCAAAGAAGTTGGAGGCCAGAAGAGTTTATATAGCAGCAAGGATAAATATTTGGCAAAACCCATTAATGAGCTTGACCTCTCCAATTGTGAGCGCTGCACTCCGAGCTATCGTCTCCTGCCAAAGAACTATCCAATACCTTCTGCTAGCCAAAGAACAGAGCTTGGTTTTGAACTATTAAATGACCACTGGGTATCTGTTACTTCTGGAAGTGAGGATTACTCTTTTAAACACATGCGCAAAAACCAGTATGAAGAAAGCCTGTTTCGGTGTGAGGATGACAGGTTTGAACTGGATATGTTGTTGGAGTCAGTGAACGTAACAACCAAGCGTGTGGAAGATCTATTAGAAAAGATCAACAACAATACAATTAAGATGGACAGTCCAATTCGTATTGAAGATCACTTCACAGCTCTGAACCTAAGGTGCATTGAGCGATTATATGGTGACCATGGGCTTGATGTGATGGACGTGTTGAGGAAAAATGCTCCACTAGCTTTGCCTGTCATATTGACCCGTTTGAAGCAAAAGCAAGAAGAGTGGGCAAGGTGTCGTTCAGATTTTAATAAAGTTTGGGCTGATATATATGCCAAGAACTATCACAAGTCACTTGATCATCGTAGCTTCTACTTTAAGCAACAAGACACCAAGAGCTTAAGCACAAAAGCCTTGTTGGCTGAGATTAAAGAGATTAGTGAGAAGAAGCGGAAGGAAGATGATGTTCTTCTTGCTATTGCTGCTGGAAATAGACGACCTATTATTCCGAATTTGGAATTTGAGTACCCTGACCCAGAAATCCATGAAGATCTGTATCAACTCGTCAAATATTCTTGTGGAGAAGTTTGCACAACCGAGCAATTGGATAAAGTTATGAAGATATGGACAACTTTTCTTGAACCAATACTAGGTGTTCCTACGCGGCCTCAAGGTGCAGAGGATACCGAAGATGGTGTGAAAGCAAAAAATCTTGCTGTAAAGAGTGGTTCTTTGAGTCCTGGAGAGAGTGATGGTAGTCCTGATGGTGATGCTACTCTGACGAATTCCAAACAGTTGAATTCATCAAGAAATGGAGATGAAAGCATTCAACCTGAACAATCAAGCTCTTGCAGGACTTGGACAGTAAATGGGGCTAATGGGGTCAAAGAAGAAAGTTGTCTCAACATTGATCATGCTGCATGTGAAGGGGATACATTGTACAATACCTCTCGACAAGGTAAAGTGCAGAGTAACACATCTCCAGCTGAGGAAACGTCTGGAGTTGGCAAACAAGACAATTTCAATGAACGATTAGTGAATTCTAATGTATCACTTGCCACTGGGTTGGAGCAAAGTAATGGAAGAACTAATGTAGACCACTCATCAGGGCATAGTTCTACTCCGTCCAGACCTGGTAATGGCACTGTTGATGTTGGGCTGGAGTTACCTTCATCAGAGGGTGGTGATTCTACAAGACCAGGTATTTCCTTAAATGGAGCAGTAGCGGAAGGCGCCGTAGGTCTCAGATATCTTGAAGAGTCTGCTAGACACTTCAAGATTGATAGAGAAGAAGGCGAGATATCTCCAAATGGAGATTTCGAGGAGGTTAATTTTGCAAATTATAGAGAAGCTGGTTCAGAGGCTGTTCAAAAACCAAAGGATGGTACTGTCAGCAGGCAATATCAAGCCAGACATGGGGAAGAAGAAATATGCGGTGGAGAGACAGGTGGAGAAAATGAAGCTGATGCTGATGATGAAGGTGAGGAAAGTGCTCCGAGGTCATCTGAGGATAGTGAGAATGCCTCTGAGAATGGTGATGTTTCTGGAAGTGAATCTGGTGACGGAGAGGAATGTTCTCGTGAAGAGCATGAGGAAGATGGGGATAATGATGAGCACGATACTAAGGCCGAGAGTGAAGGTGAAGCTGAAGGGATGGCTGATGCCCATGATGTTGAAGGAGATGGAATATCTTTGCCTCTTTCAGAACGTTTTCTCTCGACTGTGAAGCCTCTTGCAAAGTATGTTCCTCCAGCTTTACATGACAAGGAGAAGGACTCTCGGATATTTTATGGAAATGATTCTTTCTATGTGCTATTCAGGCTTCACCAAACCTTATATGAGAGAATACAATCAGCAAAGATTAACTCATCATCTGCTGAGAGGAAGTGGCGGGCTTCTTCAAATGAGACAAGCCCTACAGACTCTTATGCTAGATTCATGAATGCACTTTACAATTTACTTGACGGTTCTTCTGACAATACGAAATTTGAAGATGATTGTCGAGCCATCATTGGGACACAGTCATATCTTTTATTCACATTAGACAAGCTGATCTACAAACTCGTTAAGCAGCTCCAAACAGTTGCCAGTGAAGAGATGGACAACAAACTCGTTCAGCTTTATGCATTTGAAAAATCAAGAAAACCAGGAAGATTTGTAGATGTGGTTTATCACGAAAATGCTCGCGTTCTTCTTCATGATGAGAACATTTACCGCATCGAATGTGCATCCTCTCCAACCCAGGTGTCTATTCAGCTAATGGATTTTGGGCATGATAAGCCTGAAATGACTGCTGTTTCTATGGACCCTAATTTTTCTGCGTATCTGCATGATGAATTCCTTTCAGTTCTTCCTGACAAAAAGGAGAAGTCCGGGATCTTCTTGATGAGGAACAAACGTAAGTATGGCAGCGATGATTTGTCTGCTATCTGTGAGGCCATGGAAGGACTAAAAGTCGCTAATGGTCTGGAGTGTAAGATCGCTTACCATTCATCCAAGGTCTCATACGTTTTAGATACAGAAGACTTTCTGTTTCGGACAAAGAAGAAAAGGAAAAGTTTGCACCAGAACGGTTCATGCCATAACCTGGCTAGGTCTTCTAACGGTTCTAGTAGAGTAGAGCGATTTCAGCGGTTGCTTTCTGGCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1417

Amino Acids

160.6

Weight (kDa)

5.28

Isoelectric Point (pI)

45.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	65 - 108	3.6e-18	Paired amphipathic helix repeat
PAH	PF02671	151 - 195	4.2e-18	Paired amphipathic helix repeat
PAH	PF02671	338 - 382	4.7e-15	Paired amphipathic helix repeat
Sin3_corepress	PF08295	479 - 571	3e-35	Sin3 family co-repressor
Sin3a_C	PF16879	1129 - 1377	2.5e-47	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24190 AT1G24190 AT1G24190 AT1G70060 AT1G70060
fragaria_vesca	FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300
malus_domestica	MD13G1084700.v1.1 MD16G1083700.v1.1
prunus_persica	Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1
pyrus_communis	pycom13g07320 pycom16g07260
rosa_chinensis	RchiOBHm_Chr4g0444901
rosa_laevigata	RLG00000005790
rosa_multiflora	Rmu_sc0006845.1_g000002 Rmu_sc0013783.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385400
rosa_rugosa	Rorug04G0355800 Rorug04G0355900.1 Rorug04G0356000 Rorug04G0356100
rosa_samantha	Rh4AG417700 Rh4BG429500 Rh4CG444200 Rh4DG425400
rosa_wichuraiana	Rw4G035800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	459
Acc16I	TGCGCA	1 cut(s)	1583
AccB1I	GGYRCC	2 cut(s)	1334, 2831
AccB7I	CCANNNNNTGG	3 cut(s)	94, 2981, 4186
AccBSI	CCGCTC	1 cut(s)	62
AccI	GTMKAC	1 cut(s)	2694
AccII	CGCG	2 cut(s)	2263, 3786
AclI	AACGTT	1 cut(s)	3307
AclWI	GGATC	2 cut(s)	737, 3983
AcoI	YGGCCR	1 cut(s)	961
AcuI	CTGAAG	3 cut(s)	948, 1032, 3267
AcyI	GRCGYC	1 cut(s)	2832
AfaI	GTAC	5 cut(s)	488, 959, 2126, 2540, 2986
AfeI	AGCGCT	1 cut(s)	1439
AfiI	CCNNNNNNNGG	7 cut(s)	94, 2249, 2738, 2981, 3015, 3612, 4186
AflII	CTTAAG	1 cut(s)	1999
AflIII	ACRYGT	1 cut(s)	1815
AhdI	GACNNNNNGTC	1 cut(s)	4052
AjiI	CACGTC	1 cut(s)	1816
AloI	GAACNNNNNNTCC	2 cut(s)	688, 720
Alw21I	GWGCWC	4 cut(s)	35, 653, 3094, 3216
Alw26I	GTCTC	7 cut(s)	870, 1463, 2504, 2846, 3035, 3500, 4105
AlwI	GGATC	2 cut(s)	737, 3983
AlwNI	CAGNNNCTG	1 cut(s)	2963
Ama87I	CYCGRG	2 cut(s)	63, 1305
Aor51HI	AGCGCT	1 cut(s)	1439
AoxI	GGCC	7 cut(s)	47, 231, 961, 1360, 2264, 3225, 4035
AseI	ATTAAT	1 cut(s)	1410
Asp700I	GAANNNNTTC	7 cut(s)	204, 224, 604, 2424, 3309, 3397, 4125
AspLEI	GCGC	3 cut(s)	1440, 1584, 2834
AspS9I	GGNCC	4 cut(s)	9, 48, 696, 3904
AsuC2I	CCSGG	1 cut(s)	3973
AsuNHI	GCTAGC	1 cut(s)	1490
AvaI	CYCGRG	2 cut(s)	63, 1305
AvaII	GGWCC	3 cut(s)	9, 696, 3904
AxyI	CCTNAGG	1 cut(s)	1766
BanI	GGYRCC	2 cut(s)	1334, 2831
BanII	GRGCYC	2 cut(s)	35, 653
BarI	GAAGNNNNNNTAC	2 cut(s)	1203, 1235
BauI	CACGAG	1 cut(s)	3177
BbsI	GAAGAC	4 cut(s)	828, 936, 4128, 4188
Bbv12I	GWGCWC	4 cut(s)	35, 653, 3094, 3216
BbvCI	CCTCAGC	1 cut(s)	2586
BceAI	ACGGC	3 cut(s)	618, 976, 2819
BcgI	CGANNNNNNTGC	2 cut(s)	1286, 1320
BciVI	GTATCC	2 cut(s)	2275, 2524
BclI	TGATCA	2 cut(s)	1957, 2509
BcnI	CCSGG	1 cut(s)	3973
BcoDI	GTCTC	7 cut(s)	870, 1463, 2504, 2846, 3035, 3500, 4105
BfaI	CTAG	7 cut(s)	1491, 1838, 2249, 2865, 3531, 4191, 4209
BfmI	CTRYAG	2 cut(s)	594, 3516
BfoI	RGCGCY	2 cut(s)	1441, 2835
BfrI	CTTAAG	1 cut(s)	1999
BfuI	GTATCC	2 cut(s)	2275, 2524
BglI	GCCNNNNNGGC	2 cut(s)	79, 2018
BglII	AGATCT	2 cut(s)	1684, 2149
Bme18I	GGWCC	3 cut(s)	9, 696, 3904
BmeRI	GACNNNNNGTC	1 cut(s)	4052
BmeT110I	CYCGRG	2 cut(s)	63, 1305
BmgBI	CACGTC	1 cut(s)	1816
BmgT120I	GGNCC	4 cut(s)	9, 48, 696, 3904
BmiI	GGNNCC	4 cut(s)	1172, 1336, 2833, 3906
BmrI	ACTGGG	2 cut(s)	1543, 2670
BmsI	GCATC	8 cut(s)	127, 298, 666, 2359, 3055, 3250, 3825, 3836
BmtI	GCTAGC	1 cut(s)	1494
BmuI	ACTGGG	2 cut(s)	1543, 2670
BpiI	GAAGAC	4 cut(s)	828, 936, 4128, 4188
BplI	GAGNNNNNCTC	6 cut(s)	1550, 1582, 3107, 3139, 3359, 3391
BpmI	CTGGAG	6 cut(s)	2361, 2565, 2619, 2780, 3333, 4088
Bpu10I	CCTNAGC	2 cut(s)	142, 2586
BpuEI	CTTGAG	2 cut(s)	26, 2253
BpuMI	CCSGG	1 cut(s)	3973
BsaBI	GATNNNNATC	2 cut(s)	1260, 2154
BsaHI	GRCGYC	1 cut(s)	2832
BsaI	GGTCTC	3 cut(s)	870, 2846, 4105
BsaJI	CCNNGG	8 cut(s)	12, 51, 699, 1795, 3095, 3840, 4038, 4095
Bsc4I	CCNNNNNNNGG	7 cut(s)	94, 2249, 2738, 2981, 3015, 3612, 4186
Bse1I	ACTGG	5 cut(s)	1538, 1591, 1641, 2665, 3687
Bse21I	CCTNAGG	1 cut(s)	1766
Bse3DI	GCAATG	1 cut(s)	663
Bse8I	GATNNNNATC	2 cut(s)	1260, 2154
BseDI	CCNNGG	8 cut(s)	12, 51, 699, 1795, 3095, 3840, 4038, 4095
BseJI	GATNNNNATC	2 cut(s)	1260, 2154
BseLI	CCNNNNNNNGG	7 cut(s)	94, 2249, 2738, 2981, 3015, 3612, 4186
BseMI	GCAATG	1 cut(s)	663
BseMII	CTCAG	7 cut(s)	739, 2013, 2577, 2857, 3096, 3117, 3468
BseNI	ACTGG	5 cut(s)	1538, 1591, 1641, 2665, 3687
BseRI	GAGGAG	4 cut(s)	81, 363, 829, 2936
BsgI	GTGCAG	4 cut(s)	519, 1426, 2295, 2585
Bsh1236I	CGCG	2 cut(s)	2263, 3786
BshFI	GGCC	7 cut(s)	49, 233, 963, 1362, 2266, 3227, 4037
BshNI	GGYRCC	2 cut(s)	1334, 2831
BsiHKAI	GWGCWC	4 cut(s)	35, 653, 3094, 3216
BsiHKCI	CYCGRG	2 cut(s)	63, 1305
BsiSI	CCGG	1 cut(s)	3972
BslFI	GGGAC	2 cut(s)	29, 3628
BslI	CCNNNNNNNGG	7 cut(s)	94, 2249, 2738, 2981, 3015, 3612, 4186
BsmAI	GTCTC	7 cut(s)	870, 1463, 2504, 2846, 3035, 3500, 4105
BsmBI	CGTCTC	1 cut(s)	1463
BsmFI	GGGAC	2 cut(s)	29, 3628
BsmI	GAATGC	3 cut(s)	2424, 3124, 3547
BsnI	GGCC	7 cut(s)	49, 233, 963, 1362, 2266, 3227, 4037
Bso31I	GGTCTC	3 cut(s)	870, 2846, 4105
BsoBI	CYCGRG	2 cut(s)	63, 1305
Bsp1286I	GDGCHC	4 cut(s)	35, 653, 3094, 3216
Bsp1407I	TGTACA	2 cut(s)	486, 2538
Bsp19I	CCATGG	2 cut(s)	1795, 4038
BspANI	GGCC	7 cut(s)	49, 233, 963, 1362, 2266, 3227, 4037
BspCNI	CTCAG	7 cut(s)	738, 2014, 2578, 2856, 3097, 3118, 3469
BspFNI	CGCG	2 cut(s)	2263, 3786
BspHI	TCATGA	5 cut(s)	216, 1185, 3537, 3796, 4247
BspLI	GGNNCC	4 cut(s)	1172, 1336, 2833, 3906
BspOI	GCTAGC	1 cut(s)	1494
BspPI	GGATC	2 cut(s)	737, 3983
BspQI	GCTCTTC	1 cut(s)	3177
BspT107I	GGYRCC	2 cut(s)	1334, 2831
BspTI	CTTAAG	1 cut(s)	1999
BspTNI	GGTCTC	3 cut(s)	870, 2846, 4105
BsrBI	CCGCTC	1 cut(s)	62
BsrDI	GCAATG	1 cut(s)	663
BsrGI	TGTACA	2 cut(s)	486, 2538
BsrI	ACTGG	5 cut(s)	1538, 1591, 1641, 2665, 3687
BssECI	CCNNGG	8 cut(s)	12, 51, 699, 1795, 3095, 3840, 4038, 4095
BssNI	GRCGYC	1 cut(s)	2832
BssSI	CACGAG	1 cut(s)	3177
BssT1I	CCWWGG	3 cut(s)	1795, 4038, 4095
Bst2BI	CACGAG	1 cut(s)	3177
Bst6I	CTCTTC	6 cut(s)	829, 1361, 1875, 2850, 3177, 3687
BstACI	GRCGYC	1 cut(s)	2832
BstAFI	CTTAAG	1 cut(s)	1999
BstAUI	TGTACA	2 cut(s)	486, 2538
BstC8I	GCNNGC	6 cut(s)	35, 681, 1492, 2996, 3492, 3784
BstDSI	CCRYGG	4 cut(s)	51, 699, 1795, 4038
BstEII	GGTNACC	1 cut(s)	1790
BstFNI	CGCG	2 cut(s)	2263, 3786
BstH2I	RGCGCY	2 cut(s)	1441, 2835
BstHHI	GCGC	3 cut(s)	1440, 1584, 2834
BstMAI	GTCTC	7 cut(s)	870, 1463, 2504, 2846, 3035, 3500, 4105
BstNSI	RCATGY	4 cut(s)	683, 1152, 1582, 2523
BstPI	GGTNACC	1 cut(s)	1790
BstSFI	CTRYAG	2 cut(s)	594, 3516
BstUI	CGCG	2 cut(s)	2263, 3786
BstV2I	GAAGAC	4 cut(s)	828, 936, 4128, 4188
BstX2I	RGATCY	3 cut(s)	1684, 2149, 3975
BstXI	CCANNNNNNTGG	2 cut(s)	1678, 2665
BstYI	RGATCY	3 cut(s)	1684, 2149, 3975
Bsu36I	CCTNAGG	1 cut(s)	1766
BsuI	GTATCC	2 cut(s)	2275, 2524
BsuRI	GGCC	7 cut(s)	49, 233, 963, 1362, 2266, 3227, 4037
BtgI	CCRYGG	4 cut(s)	51, 699, 1795, 4038
BtgZI	GCGATG	1 cut(s)	4026
BtrI	CACGTC	1 cut(s)	1816
BtsI	GCAGTG	1 cut(s)	246
BtsIMutI	CAGTG	9 cut(s)	246, 733, 933, 1236, 1531, 1662, 2658, 2742, 3694
Cac8I	GCNNGC	6 cut(s)	35, 681, 1492, 2996, 3492, 3784
CaiI	CAGNNNCTG	1 cut(s)	2963
CciI	TCATGA	5 cut(s)	216, 1185, 3537, 3796, 4247
CfoI	GCGC	3 cut(s)	1440, 1584, 2834
Cfr13I	GGNCC	4 cut(s)	9, 48, 696, 3904
CsiI	ACCWGGT	2 cut(s)	2731, 2797
Csp6I	GTAC	5 cut(s)	487, 958, 2125, 2539, 2985
CspCI	CAANNNNNGTGG	6 cut(s)	223, 258, 1824, 1859, 1865, 1900
CviQI	GTAC	5 cut(s)	487, 958, 2125, 2539, 2985
DinI	GGCGCC	1 cut(s)	2833
DraI	TTTAAA	2 cut(s)	283, 1573
DriI	GACNNNNNGTC	1 cut(s)	4052
EaeI	YGGCCR	1 cut(s)	961
Eam1104I	CTCTTC	6 cut(s)	829, 1361, 1875, 2850, 3177, 3687
Eam1105I	GACNNNNNGTC	1 cut(s)	4052
EarI	CTCTTC	6 cut(s)	829, 1361, 1875, 2850, 3177, 3687
Ecl136II	GAGCTC	2 cut(s)	33, 651
Eco130I	CCWWGG	3 cut(s)	1795, 4038, 4095
Eco24I	GRGCYC	2 cut(s)	35, 653
Eco31I	GGTCTC	3 cut(s)	870, 2846, 4105
Eco32I	GATATC	3 cut(s)	1112, 2849, 2901
Eco47I	GGWCC	3 cut(s)	9, 696, 3904
Eco47III	AGCGCT	1 cut(s)	1439
Eco53kI	GAGCTC	2 cut(s)	33, 651
Eco57I	CTGAAG	3 cut(s)	948, 1032, 3267
Eco81I	CCTNAGG	1 cut(s)	1766
Eco88I	CYCGRG	2 cut(s)	63, 1305
Eco91I	GGTNACC	1 cut(s)	1790
EcoICRI	GAGCTC	2 cut(s)	33, 651
EcoO65I	GGTNACC	1 cut(s)	1790
EcoRI	GAATTC	4 cut(s)	2383, 2398, 2638, 3935
EcoRV	GATATC	3 cut(s)	1112, 2849, 2901
EcoT14I	CCWWGG	3 cut(s)	1795, 4038, 4095
EcoT22I	ATGCAT	2 cut(s)	681, 3727
EcoT38I	GRGCYC	2 cut(s)	35, 653
EgeI	GGCGCC	1 cut(s)	2833
EheI	GGCGCC	1 cut(s)	2833
ErhI	CCWWGG	3 cut(s)	1795, 4038, 4095
Esp3I	CGTCTC	1 cut(s)	1463
FalI	AAGNNNNNCTT	2 cut(s)	192, 224
FaqI	GGGAC	2 cut(s)	29, 3628
FauI	CCCGC	2 cut(s)	244, 3483
FbaI	TGATCA	2 cut(s)	1957, 2509
FblI	GTMKAC	1 cut(s)	2694
FriOI	GRGCYC	2 cut(s)	35, 653
FspBI	CTAG	7 cut(s)	1491, 1838, 2249, 2865, 3531, 4191, 4209
FspI	TGCGCA	1 cut(s)	1583
GlaI	GCGC	3 cut(s)	1439, 1583, 2833
GsuI	CTGGAG	6 cut(s)	2361, 2565, 2619, 2780, 3333, 4088
HaeII	RGCGCY	2 cut(s)	1441, 2835
HaeIII	GGCC	7 cut(s)	49, 233, 963, 1362, 2266, 3227, 4037
HapII	CCGG	1 cut(s)	3972
HhaI	GCGC	3 cut(s)	1440, 1584, 2834
Hin1I	GRCGYC	1 cut(s)	2832
Hin6I	GCGC	3 cut(s)	1438, 1582, 2832
HinP1I	GCGC	3 cut(s)	1438, 1582, 2832
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HpaII	CCGG	1 cut(s)	3972
Hpy166II	GTNNAC	6 cut(s)	418, 520, 573, 733, 1660, 2695
Hpy8I	GTNNAC	6 cut(s)	418, 520, 573, 733, 1660, 2695
Hpy99I	CGWCG	1 cut(s)	449
HpyCH4IV	ACGT	6 cut(s)	1662, 1815, 2594, 3307, 3997, 4107
HpySE526I	ACGT	6 cut(s)	1662, 1815, 2594, 3307, 3997, 4107
Hsp92I	GRCGYC	1 cut(s)	2832
HspAI	GCGC	3 cut(s)	1438, 1582, 2832
KasI	GGCGCC	1 cut(s)	2831
Ksp22I	TGATCA	2 cut(s)	1957, 2509
LguI	GCTCTTC	1 cut(s)	3177
LmnI	GCTCC	6 cut(s)	648, 1837, 2668, 2816, 3097, 3678
LweI	GCATC	8 cut(s)	127, 298, 666, 2359, 3055, 3250, 3825, 3836
MabI	ACCWGGT	2 cut(s)	2731, 2797
MaeI	CTAG	7 cut(s)	1491, 1838, 2249, 2865, 3531, 4191, 4209
MaeII	ACGT	6 cut(s)	1662, 1815, 2594, 3307, 3997, 4107
MaeIII	GTNAC	8 cut(s)	999, 1543, 1663, 1790, 1950, 2570, 2763, 3158
MbiI	CCGCTC	1 cut(s)	62
MfeI	CAATTG	3 cut(s)	941, 1429, 2201
MflI	RGATCY	3 cut(s)	1684, 2149, 3975
MhlI	GDGCHC	4 cut(s)	35, 653, 3094, 3216
Mly113I	GGCGCC	1 cut(s)	2832
MlyI	GAGTC	5 cut(s)	1661, 2344, 2867, 3368, 3515
MmeI	TCCRAC	4 cut(s)	1336, 1629, 2646, 3860
Mph1103I	ATGCAT	2 cut(s)	681, 3727
MroXI	GAANNNNTTC	7 cut(s)	204, 224, 604, 2424, 3309, 3397, 4125
MslI	CAYNNNNRTG	1 cut(s)	3777
MspA1I	CMGCKG	2 cut(s)	2585, 4231
MspCI	CTTAAG	1 cut(s)	1999
MspI	CCGG	1 cut(s)	3972
MunI	CAATTG	3 cut(s)	941, 1429, 2201
Mva1269I	GAATGC	3 cut(s)	2424, 3124, 3547
MvnI	CGCG	2 cut(s)	2263, 3786
NarI	GGCGCC	1 cut(s)	2832
NciI	CCSGG	1 cut(s)	3973
NcoI	CCATGG	2 cut(s)	1795, 4038
NheI	GCTAGC	1 cut(s)	1490
NlaIV	GGNNCC	4 cut(s)	1172, 1336, 2833, 3906
NmeAIII	GCCGAG	1 cut(s)	3253
NmuCI	GTSAC	3 cut(s)	1790, 1950, 3158
NsbI	TGCGCA	1 cut(s)	1583
NsiI	ATGCAT	2 cut(s)	681, 3727
NspI	RCATGY	4 cut(s)	683, 1152, 1582, 2523
PaeI	GCATGC	1 cut(s)	683
PaeR7I	CTCGAG	2 cut(s)	63, 1305
PagI	TCATGA	5 cut(s)	216, 1185, 3537, 3796, 4247
PciSI	GCTCTTC	1 cut(s)	3177
PctI	GAATGC	3 cut(s)	2424, 3124, 3547
PdmI	GAANNNNTTC	7 cut(s)	204, 224, 604, 2424, 3309, 3397, 4125
PfeI	GAWTC	5 cut(s)	1344, 2786, 3152, 3398, 3534
PflFI	GACNNNGTC	1 cut(s)	3619
PflMI	CCANNNNNTGG	3 cut(s)	94, 2981, 4186
PfoI	TCCNGGA	2 cut(s)	2338, 3971
PleI	GAGTC	5 cut(s)	1660, 2343, 2866, 3368, 3515
PluTI	GGCGCC	1 cut(s)	2835
PpsI	GAGTC	5 cut(s)	1660, 2343, 2866, 3368, 3515
PshBI	ATTAAT	1 cut(s)	1410
PsiI	TTATAA	1 cut(s)	459
Psp124BI	GAGCTC	2 cut(s)	35, 653
Psp1406I	AACGTT	1 cut(s)	3307
PspEI	GGTNACC	1 cut(s)	1790
PspN4I	GGNNCC	4 cut(s)	1172, 1336, 2833, 3906
PspPI	GGNCC	4 cut(s)	9, 48, 696, 3904
PspXI	VCTCGAGB	1 cut(s)	63
PstNI	CAGNNNCTG	1 cut(s)	2963
PsuI	RGATCY	3 cut(s)	1684, 2149, 3975
PsyI	GACNNNGTC	1 cut(s)	3619
PvuII	CAGCTG	1 cut(s)	2585
RsaI	GTAC	5 cut(s)	488, 959, 2126, 2540, 2986
RsaNI	GTAC	5 cut(s)	487, 958, 2125, 2539, 2985
RseI	CAYNNNNRTG	1 cut(s)	3777
SacI	GAGCTC	2 cut(s)	35, 653
SapI	GCTCTTC	1 cut(s)	3177
Sau96I	GGNCC	4 cut(s)	9, 48, 696, 3904
SchI	GAGTC	5 cut(s)	1661, 2344, 2867, 3368, 3515
SduI	GDGCHC	4 cut(s)	35, 653, 3094, 3216
SexAI	ACCWGGT	2 cut(s)	2731, 2797
SfaNI	GCATC	8 cut(s)	127, 298, 666, 2359, 3055, 3250, 3825, 3836
SfcI	CTRYAG	2 cut(s)	594, 3516
SfoI	GGCGCC	1 cut(s)	2833
Sfr274I	CTCGAG	2 cut(s)	63, 1305
SinI	GGWCC	3 cut(s)	9, 696, 3904
SlaI	CTCGAG	2 cut(s)	63, 1305
SmiMI	CAYNNNNRTG	1 cut(s)	3777
SmlI	CTYRAG	5 cut(s)	41, 63, 1305, 1999, 2268
SmoI	CTYRAG	5 cut(s)	41, 63, 1305, 1999, 2268
SphI	GCATGC	1 cut(s)	683
SspDI	GGCGCC	1 cut(s)	2831
SspI	AATATT	3 cut(s)	303, 1394, 2171
SspMI	CTAG	7 cut(s)	1491, 1838, 2249, 2865, 3531, 4191, 4209
SstI	GAGCTC	2 cut(s)	35, 653
StyI	CCWWGG	3 cut(s)	1795, 4038, 4095
TaiI	ACGT	6 cut(s)	1665, 1818, 2597, 3310, 4000, 4110
TaqI	TCGA	9 cut(s)	64, 292, 1134, 1306, 2551, 2919, 3317, 3601, 3819
TatI	WGTACW	2 cut(s)	486, 2538
TauI	GCSGC	3 cut(s)	49, 673, 2266
TfiI	GAWTC	5 cut(s)	1344, 2786, 3152, 3398, 3534
TscAI	CASTG	9 cut(s)	253, 733, 940, 1236, 1538, 1662, 2665, 2749, 3694
TseFI	GTSAC	3 cut(s)	1790, 1950, 3158
Tsp45I	GTSAC	3 cut(s)	1790, 1950, 3158
TspGWI	ACGGA	2 cut(s)	2712, 3177
TspRI	CASTG	9 cut(s)	253, 733, 940, 1236, 1538, 1662, 2665, 2749, 3694
Tth111I	GACNNNGTC	1 cut(s)	3619
Van91I	CCANNNNNTGG	3 cut(s)	94, 2981, 4186
Vha464I	CTTAAG	1 cut(s)	1999
VpaK11BI	GGWCC	3 cut(s)	9, 696, 3904
VspI	ATTAAT	1 cut(s)	1410
XceI	RCATGY	4 cut(s)	683, 1152, 1582, 2523
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3694
XhoI	CTCGAG	2 cut(s)	63, 1305
XmiI	GTMKAC	1 cut(s)	2694
XmnI	GAANNNNTTC	7 cut(s)	204, 224, 604, 2424, 3309, 3397, 4125
XspI	CTAG	7 cut(s)	1491, 1838, 2249, 2865, 3531, 4191, 4209
Zsp2I	ATGCAT	2 cut(s)	681, 3727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.