Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0002013

Physical Location & Seq

Genomic Coordinates Reverse (-)

57239930 .. 57247491

Gene Structure 7562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG429500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4254 bp

ATGAAGAGGTCTCGTGACGACGGTTATACAAGTTCGCAAGTTAAACGGCCCATGGTTACCTCTCGAGGCGAGCCCTCGGGGCAGCCGCAGATGATGACAGCAACAGCTTCCCAGAAACTAACAACCAATGATGCCTTATCATATCTAAAGGCGGTGAAGGAGATATTTGAAAACAACAAGGAAAAATACGAAGACTTTCTTGAAGTTATGAAAGACTTCAAAGCTCAGAGAGTTGACACCTCGGGTGTGATACAGAGGGTAAAGGATTTATTTAAAGGGCATCGAGACCTAATATTAGGCTTCAATACCTTCTTGCCGAAGGGATATGAAATCACTCTCCCTCTCGATGACGAACAACCTCCTCAAAAGAAGCCCGTTGAATTTGAAGAAGCTATCAGTTTTGTCAACAAAATTAAGACTCGATTTCAACATGATGATCATGTTTATAAATCCTTTTTGGACATTTTGAATATGTACAGAAAGGAAAACAAGTCCATCTCTGAAGTTTACCAGGAGGTCTCAGCACTTTTTCAAGACCACCCAGACTTACTTGTGGAGTTTACTCACTTTTTACCTGATACTTCAGCAACAGCCTCCACCCAGTTTGCTCCATCTCAAAGAAACTCAATGCTTCGTGATAGGAGCTCTGCCATGCCCCCTATGAGGCAAATGCATGTTGATAAGAAAGAAAGGCCTATGGGTTCTTACCCTGAGCATGATCTCAGTGTTGACCGTCCTGATCTGGACCATGATAGAGCTCTGATGAAAGCTGAGAAAGAGCAAAGGAGGCGTGGTGAAAAAGAAAAGGAGAGGAGAGAAGACAGAGAAAGACGAGATGATAGGGATTTTGACCATGATGGCAGTAGGGACTTCAACATGCAGCGTTTTCCCCACAAACGGAAATCCACTCGTAGGGGTGAAGACTTGGCTGCTGATCAATTGCATCAAGGTATATATGGCTCGGAGAGTGCCTTCTGTGAGAAGGTGAAGGAGAAGTTACGAAATCCTGATGATTACCAGGAGTTTTTGAAGTGCCTCCATATTTATAGCAAGGAAATTATTACCCGGGCTGAATTGCAGTCATTGGTGGGTGATTTACTTGGAAAATATCCAGATCTTATGGATGGGTTCAATGAATTTTTGTCATGTTGTGAGAAGAAAGATGGGTTCCTTGCTGGTGTCATGAGTAAAAAATCTTTGTGGAATGAAGGCAATCTACCAAGACCAGTGAAGGTAGAGGATAAGGAGAAAGATCGAGATCGTGAAAGGGATGATGTGGTTAGAGATAGAGAGCGTGAAAACCGAGAAAGGGATAGACCTGATAGAAATGGTGCCTTTGGAAATAAAGAAATTGGAGGCCAGAAGATCTCTATATTTTCCGGCAAGGACAAATATTTGGCAAAACCTATTAATGAGCTTGACCTCTCTAATTGTGAGCGTTGCACTCCGAGCTATCGTCTTCTGCCAAAGAATTATCCAATACCTTCTGCTAGCCAAAGAACCGAACTTGGGTCTGAAGTACTAAATGATCATTGGGTGTCTGTTACTTCTGGAAGCGAGGATTATTCTTTTAAACACATGCGCAAAAACCAGTATGAAGAAAGCCTGTTTCGGTGTGAAGATGATAGGTTTGAATTAGATATGCTGTTAGAGTCTGTGAATGTAACAACCAAGCGTGTGGAAGAACTATTAGAGAAGATCAACAACAATACCATTAAGACCGAGAGTCCAATCCGTGTAGAAGAATACTTCACAGCTCTGAATCTAAGGTGTGTTGAGCGATTATATGGTGACCACGGGCTTGATGTAATGGATGTGTTGAGGAAAAATGCTCCACTAGCTTTGCCTGTTATATTAACGCGCTTGAAGCAAAAGCAAGAAGAGTGGGCAAGGTGTCGCTCTGATTTTAACAAAGTCTGGGCTGATATATATGCCAAGAACTACCACAAGTCGCTTGATCATCGTAGCTTCTACTTCAAGCAACAGGACTCAAAGAGCTTAAGCACCAAAGCCTTGTTGGCAGAGATTAAAGAGATGAGTGAGAAGAAGCGGAAGGAAGATGATGTTCTTCTTGCTATTGCTGCTGGAAATAGACGTCCTATTATTCCAAATTTGGAATTCGAGTACCCTGATCCGGACATCCATGAAGATCTGTATCAACTCATTAAATATTCTTGTGGAGAAGTTTGCACAACCGAGCAATTGGACAAAGTTATGAAGATATGGACAACCTTCCTTGAGCCAGTGCTTGGTGTTCCTCCTCGGCCTCAGGTTGCAGAGGATACTGAAGATGTTGTGAAGGCAAAAACTCATGCTGGCAAAAATGGTGCTGTGAGTGGGGGAGAGAGTGATGATAATCCTGATGGTGGGGCTACTGTGACAAATTCTAAACAGTTAAATTCATCGAGAAATGGAGACGAAAGCATTCAACCTGAACAATCAAGCTCTGCAAGAGCTTGGACAGTAAATGGAGCTAATGGGCTCAAAGAAGAAAGTTCTCATGATATTGATCGTGCTACATGTAAAGGTGATACTTTCTGCAATACCTCTCAACAAGGTAAAGTGCAAAGTAATGCATCTACAGCCGATGAAATGTCTGGAGTGAGCAAACAAGACAATTTTAATGAACGATTAGTCAATTCTAATGTATCACTTGCTACTGGCTTGGAGCAAAGCAATGGAAGAACTAATGTGGAAAACTCATCAGGTCTTAGTCCTACTCCTTCCAGACCTGGAAATGGCACTGTTGAGGGTGGGCTTGAGTTACCTTCACGGTCTTCACCAGAGGGTGGTGATTCTACTAGACCAGTTATATCCTCAAATGGAGCAATCACAGAAGCCACGAAAGGTCACAGATATGTGGAAGAGTCGGTTAGACACTTCAAGATTGAAAGAGAAGAAGGTGAAATATCTCCAAATGGAGATTTTGAGGAGGATAATTTTGCAAACTATAGAGAAGCCGGTTCAGAGGCTGTTCAAAAACCAAAGGATGGTGTTTCCAGCAGGCAACTTAAAGGCAGGCATGGGGAAGAAGAAATATGTGGTGGAGATGCAGGTGGAGAAAATGAGGCTGATGCTGATGATGAAGGTGAGGAAAGTGCTCATAGATCATCAGAGGATAGTGAGAATGCTTCTGAGAATGGCGATGTCTCTGGAAGTGAATCTGGTGAGGGAGAGGAATGTTCTCGAGAAGAGCGTGAGGAAGATGGGGACCATGATGAGCATGATACTAAGGCCGAGAGTGAAGGTGAAGCTGAAGGGACGGCCGACGCCCATGATGTTGAAGGAGATGGAACTTCTTTACCGCACTCAGAACGTTTTCTACTTTCTGTGAAGCCTCTTGCAAAACATGTTCTTCCAGCTTTACATGACAAGGACAAGGACTCAAGGATTTTCTATGGAAACGATTCTTTCTATGTACTTTTTAGGCTTCACCAAACACTATATGAGAGAATACAATCAGCAAAGATTAACTCGTCATCAGCGGAGAAGAAGTGGCGGGCATCAAATGATACAAGCACTTCTGATTCGTATGCTAGATTCATGAATGCCCTTTACAATTTACTTGACGGTTCTTCTGACAATACAAAATTTGAGGATGATTGTCGAGCTATCATAGGGACTCAGTCATATCTTCTTTTCACATTAGACAAGCTGATCTACAAGCTTGTTAAACAGCTCCAAACAGTCGCCAGTGACGAGATGGATAATAAACTTGTCCAACTATATGCATTTGAAAAATCAAGAAAACCAGGAAGATTTGTGGATGTAGTTTATCACGAAAATGCTCGTGTTCTTCTTCACGATGAGAACATTTATCGCATAGAATGTTTCTCTTTGCCAACCCGGGTGTCTATTCAGCTTATGGATTATGGGAATGATAAGCCTGAAATGACTGCTGTTTCAATGGACCCTAATTTTTCTGCTTATTTGCACAATGATTTTCTCACTGTTCTTCCTGACAAAAGAGAGAAGTCTGGGATCTTCTTGAAGAGGAACAAACGCAAATATGCGAGTTGTGATGATTTGTCTGCTATTTGTCAGGCCATGGAAGGACTTAAAGTCGCCAATGGTCTGGAGTGTAAGATAGCGTGTCATTCATCCAAGGTATCATATGTTTTAGATACAGAAGACTTTTTGTTTCGGACAAAAAGGAGAAGAAAAACTTCGAACAGTTCCTGCCATAACGAGGCTAGGTCTTCTAGTGGTTCAAGTAGAGTAGAGCGGTTTCACCGGTTGCTCTCTGGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1417

Amino Acids

160.93

Weight (kDa)

5.44

Isoelectric Point (pI)

45.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	61 - 105	3e-18	Paired amphipathic helix repeat
PAH	PF02671	147 - 191	2.3e-18	Paired amphipathic helix repeat
PAH	PF02671	338 - 381	1.5e-16	Paired amphipathic helix repeat
Sin3_corepress	PF08295	479 - 571	1.2e-36	Sin3 family co-repressor
Sin3a_C	PF16879	1132 - 1380	1.3e-48	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24190 AT1G24190 AT1G24190 AT1G70060 AT1G70060
fragaria_vesca	FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300
malus_domestica	MD13G1084700.v1.1 MD16G1083700.v1.1
prunus_persica	Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1
pyrus_communis	pycom13g07320 pycom16g07260
rosa_chinensis	RchiOBHm_Chr4g0444901
rosa_laevigata	RLG00000005790
rosa_multiflora	Rmu_sc0006845.1_g000002 Rmu_sc0013783.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385400
rosa_rugosa	Rorug04G0355800 Rorug04G0355900.1 Rorug04G0356000 Rorug04G0356100
rosa_samantha	Rh4AG417700 Rh4BG429500 Rh4CG444200 Rh4DG425400
rosa_wichuraiana	Rw4G035800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
AarI	CACCTGC	1 cut(s)	3044
AatII	GACGTC	1 cut(s)	2098
Acc16I	TGCGCA	1 cut(s)	1583
Acc36I	ACCTGC	1 cut(s)	3044
AccB1I	GGYRCC	1 cut(s)	1331
AccB7I	CCANNNNNTGG	3 cut(s)	2249, 2789, 2990
AccBSI	CCGCTC	1 cut(s)	4225
AccII	CGCG	1 cut(s)	1861
AccIII	TCCGGA	1 cut(s)	2134
AciI	CCGC	7 cut(s)	86, 152, 2050, 3305, 3485, 3499, 4225
AclI	AACGTT	1 cut(s)	3316
AclWI	GGATC	2 cut(s)	2126, 3989
AcoI	YGGCCR	1 cut(s)	3264
AcsI	RAATTY	7 cut(s)	380, 1136, 2110, 2117, 2383, 2398, 3590
AcuI	CTGAAG	5 cut(s)	522, 567, 1536, 2307, 3276
AcyI	GRCGYC	2 cut(s)	2095, 3270
AfaI	GTAC	4 cut(s)	476, 1521, 2126, 3420
AflII	CTTAAG	1 cut(s)	1999
AflIII	ACRYGT	2 cut(s)	2519, 3349
AgeI	ACCGGT	1 cut(s)	4233
AhdI	GACNNNNNGTC	2 cut(s)	1725, 4061
AjnI	CCWGG	4 cut(s)	510, 1017, 2731, 3751
Alw21I	GWGCWC	3 cut(s)	647, 760, 3103
Alw26I	GTCTC	5 cut(s)	15, 279, 523, 2409, 3154
AlwI	GGATC	2 cut(s)	2126, 3989
AlwNI	CAGNNNCTG	2 cut(s)	2972, 4179
Ama87I	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
Aor13HI	TCCGGA	1 cut(s)	2134
AoxI	GGCC	7 cut(s)	47, 692, 1357, 2264, 3234, 3264, 4044
ApeKI	GCWGC	4 cut(s)	82, 880, 929, 2081
ApoI	RAATTY	7 cut(s)	380, 1136, 2110, 2117, 2383, 2398, 3590
AseI	ATTAAT	1 cut(s)	1410
AsiGI	ACCGGT	1 cut(s)	4233
Asp700I	GAANNNNTTC	6 cut(s)	195, 215, 2424, 3318, 3406, 4165
AspLEI	GCGC	2 cut(s)	1584, 1863
AspS9I	GGNCC	4 cut(s)	48, 745, 3211, 3910
AsuC2I	CCSGG	4 cut(s)	1066, 1067, 3847, 3848
AsuII	TTCGAA	1 cut(s)	4169
AsuNHI	GCTAGC	1 cut(s)	1490
AvaI	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
AvaII	GGWCC	3 cut(s)	745, 3211, 3910
AxyI	CCTNAGG	1 cut(s)	2268
BanI	GGYRCC	1 cut(s)	1331
BanII	GRGCYC	4 cut(s)	75, 647, 760, 2484
BarI	GAAGNNNNNNTAC	2 cut(s)	1200, 1232
BauI	CACGAG	2 cut(s)	12, 3789
BbsI	GAAGAC	7 cut(s)	198, 825, 927, 1451, 2769, 4137, 4191
Bbv12I	GWGCWC	3 cut(s)	647, 760, 3103
BbvI	GCAGC	4 cut(s)	94, 892, 916, 2068
BceAI	ACGGC	2 cut(s)	62, 3279
BciT130I	CCWGG	4 cut(s)	512, 1019, 2733, 3753
BciVI	GTATCC	1 cut(s)	2275
BclI	TGATCA	4 cut(s)	436, 934, 1528, 1957
BcnI	CCSGG	4 cut(s)	1066, 1067, 3847, 3848
BcoDI	GTCTC	5 cut(s)	15, 279, 523, 2409, 3154
BfaI	CTAG	6 cut(s)	1491, 1838, 2802, 3537, 4194, 4203
BfmI	CTRYAG	2 cut(s)	2580, 2950
BfrI	CTTAAG	1 cut(s)	1999
BfuAI	ACCTGC	1 cut(s)	3044
BfuI	GTATCC	1 cut(s)	2275
BglI	GCCNNNNNGGC	2 cut(s)	79, 2018
BglII	AGATCT	3 cut(s)	1114, 1365, 2149
BisI	GCNGC	5 cut(s)	83, 86, 881, 930, 2082
BlsI	GCNGC	5 cut(s)	84, 87, 882, 931, 2083
BmcAI	AGTACT	1 cut(s)	1521
Bme1390I	CCNGG	8 cut(s)	512, 1019, 1066, 1067, 2733, 3753, 3847, 3848
Bme18I	GGWCC	3 cut(s)	745, 3211, 3910
BmeRI	GACNNNNNGTC	2 cut(s)	1725, 4061
BmeT110I	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
BmgT120I	GGNCC	4 cut(s)	48, 745, 3211, 3910
BmiI	GGNNCC	4 cut(s)	1169, 1333, 3212, 3912
BmrFI	CCNGG	8 cut(s)	512, 1019, 1066, 1067, 2733, 3753, 3847, 3848
BmrI	ACTGGG	1 cut(s)	595
BmsI	GCATC	7 cut(s)	121, 289, 952, 2585, 3040, 3064, 3512
BmtI	GCTAGC	1 cut(s)	1494
BmuI	ACTGGG	1 cut(s)	595
BpiI	GAAGAC	7 cut(s)	198, 825, 927, 1451, 2769, 4137, 4191
BpmI	CTGGAG	2 cut(s)	2619, 4097
Bpu10I	CCTNAGC	1 cut(s)	711
Bpu14I	TTCGAA	1 cut(s)	4169
BpuEI	CTTGAG	3 cut(s)	2258, 2780, 3370
BpuMI	CCSGG	4 cut(s)	1066, 1067, 3847, 3848
BsaBI	GATNNNNATC	4 cut(s)	1257, 2154, 2355, 2508
BsaHI	GRCGYC	2 cut(s)	2095, 3270
BsaI	GGTCTC	3 cut(s)	15, 279, 523
BsaJI	CCNNGG	9 cut(s)	51, 75, 240, 1065, 1795, 2261, 3846, 4047, 4104
BsaWI	WCCGGW	2 cut(s)	2134, 4233
BsaXI	ACNNNNNCTCC	4 cut(s)	778, 808, 3479, 3509
Bse118I	RCCGGY	2 cut(s)	2960, 4233
Bse1I	ACTGG	7 cut(s)	601, 1226, 1591, 2243, 2665, 2807, 3693
Bse21I	CCTNAGG	1 cut(s)	2268
Bse3DI	GCAATG	1 cut(s)	2683
Bse8I	GATNNNNATC	4 cut(s)	1257, 2154, 2355, 2508
BseAI	TCCGGA	1 cut(s)	2134
BseBI	CCWGG	4 cut(s)	512, 1019, 2733, 3753
BseDI	CCNNGG	9 cut(s)	51, 75, 240, 1065, 1795, 2261, 3846, 4047, 4104
BseGI	GGATG	8 cut(s)	1129, 1276, 1819, 2139, 2995, 3604, 3772, 4100
BseJI	GATNNNNATC	4 cut(s)	1257, 2154, 2355, 2508
BseMI	GCAATG	1 cut(s)	2683
BseMII	CTCAG	9 cut(s)	239, 534, 702, 736, 762, 2282, 3126, 3324, 3638
BseNI	ACTGG	7 cut(s)	601, 1226, 1591, 2243, 2665, 2807, 3693
BseRI	GAGGAG	4 cut(s)	351, 826, 2250, 2945
BseX3I	CGGCCG	1 cut(s)	3264
BseXI	GCAGC	4 cut(s)	94, 892, 916, 2068
Bsh1236I	CGCG	1 cut(s)	1861
Bsh1285I	CGRYCG	1 cut(s)	3267
BshFI	GGCC	7 cut(s)	49, 694, 1359, 2266, 3236, 3266, 4046
BshNI	GGYRCC	1 cut(s)	1331
BshTI	ACCGGT	1 cut(s)	4233
BsiEI	CGRYCG	1 cut(s)	3267
BsiHKAI	GWGCWC	3 cut(s)	647, 760, 3103
BsiHKCI	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
BsiSI	CCGG	6 cut(s)	1066, 1380, 2135, 2961, 3847, 4234
BslFI	GGGAC	4 cut(s)	881, 3224, 3274, 3634
BsmAI	GTCTC	5 cut(s)	15, 279, 523, 2409, 3154
BsmBI	CGTCTC	1 cut(s)	2409
BsmFI	GGGAC	4 cut(s)	881, 3224, 3274, 3634
BsmI	GAATGC	3 cut(s)	2424, 3133, 3553
BsnI	GGCC	7 cut(s)	49, 694, 1359, 2266, 3236, 3266, 4046
Bso31I	GGTCTC	3 cut(s)	15, 279, 523
BsoBI	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
Bsp119I	TTCGAA	1 cut(s)	4169
Bsp1286I	GDGCHC	5 cut(s)	75, 647, 760, 2484, 3103
Bsp13I	TCCGGA	1 cut(s)	2134
Bsp1407I	TGTACA	1 cut(s)	474
Bsp19I	CCATGG	2 cut(s)	51, 4047
BspACI	CCGC	7 cut(s)	86, 152, 2050, 3305, 3485, 3499, 4225
BspANI	GGCC	7 cut(s)	49, 694, 1359, 2266, 3236, 3266, 4046
BspCNI	CTCAG	9 cut(s)	238, 533, 703, 735, 763, 2281, 3127, 3323, 3637
BspEI	TCCGGA	1 cut(s)	2134
BspFNI	CGCG	1 cut(s)	1861
BspHI	TCATGA	4 cut(s)	1182, 2500, 3543, 4250
BspLI	GGNNCC	4 cut(s)	1169, 1333, 3212, 3912
BspMI	ACCTGC	1 cut(s)	3044
BspOI	GCTAGC	1 cut(s)	1494
BspPI	GGATC	2 cut(s)	2126, 3989
BspQI	GCTCTTC	1 cut(s)	3186
BspT104I	TTCGAA	1 cut(s)	4169
BspT107I	GGYRCC	1 cut(s)	1331
BspTI	CTTAAG	1 cut(s)	1999
BspTNI	GGTCTC	3 cut(s)	15, 279, 523
BsrBI	CCGCTC	1 cut(s)	4225
BsrDI	GCAATG	1 cut(s)	2683
BsrFI	RCCGGY	2 cut(s)	2960, 4233
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	7 cut(s)	601, 1226, 1591, 2243, 2665, 2807, 3693
BssAI	RCCGGY	2 cut(s)	2960, 4233
BssECI	CCNNGG	9 cut(s)	51, 75, 240, 1065, 1795, 2261, 3846, 4047, 4104
BssNI	GRCGYC	2 cut(s)	2095, 3270
BssSI	CACGAG	2 cut(s)	12, 3789
BssT1I	CCWWGG	3 cut(s)	51, 4047, 4104
Bst2BI	CACGAG	2 cut(s)	12, 3789
Bst2UI	CCWGG	4 cut(s)	512, 1019, 2733, 3753
Bst6I	CTCTTC	4 cut(s)	1875, 2859, 3186, 3986
BstACI	GRCGYC	2 cut(s)	2095, 3270
BstAFI	CTTAAG	1 cut(s)	1999
BstAUI	TGTACA	1 cut(s)	474
BstBI	TTCGAA	1 cut(s)	4169
BstC8I	GCNNGC	6 cut(s)	71, 1492, 2317, 3005, 3020, 3501
BstDSI	CCRYGG	3 cut(s)	51, 1795, 4047
BstEII	GGTNACC	2 cut(s)	55, 1790
BstF5I	GGATG	8 cut(s)	1129, 1276, 1819, 2139, 2995, 3604, 3772, 4100
BstFNI	CGCG	1 cut(s)	1861
BstHHI	GCGC	2 cut(s)	1584, 1863
BstMAI	GTCTC	5 cut(s)	15, 279, 523, 2409, 3154
BstMCI	CGRYCG	1 cut(s)	3267
BstMWI	GCNNNNNNNGC	8 cut(s)	79, 787, 1449, 1838, 1867, 2018, 2081, 2582
BstNI	CCWGG	4 cut(s)	512, 1019, 2733, 3753
BstNSI	RCATGY	5 cut(s)	677, 880, 1582, 2523, 3353
BstPI	GGTNACC	2 cut(s)	55, 1790
BstSCI	CCNGG	8 cut(s)	510, 1017, 1064, 1065, 2731, 3751, 3845, 3846
BstSFI	CTRYAG	2 cut(s)	2580, 2950
BstUI	CGCG	1 cut(s)	1861
BstV1I	GCAGC	4 cut(s)	94, 892, 916, 2068
BstV2I	GAAGAC	7 cut(s)	198, 825, 927, 1451, 2769, 4137, 4191
BstX2I	RGATCY	4 cut(s)	1114, 1365, 2149, 3981
BstXI	CCANNNNNNTGG	2 cut(s)	1678, 4075
BstYI	RGATCY	4 cut(s)	1114, 1365, 2149, 3981
BstZI	CGGCCG	1 cut(s)	3264
Bsu36I	CCTNAGG	1 cut(s)	2268
BsuI	GTATCC	1 cut(s)	2275
BsuRI	GGCC	7 cut(s)	49, 694, 1359, 2266, 3236, 3266, 4046
BtgI	CCRYGG	3 cut(s)	51, 1795, 4047
BtgZI	GCGATG	1 cut(s)	3159
BtsCI	GGATG	8 cut(s)	1129, 1276, 1819, 2139, 2995, 3604, 3772, 4100
BtsIMutI	CAGTG	6 cut(s)	730, 1233, 2250, 2742, 3700, 3948
BveI	ACCTGC	1 cut(s)	3044
Cac8I	GCNNGC	6 cut(s)	71, 1492, 2317, 3005, 3020, 3501
CaiI	CAGNNNCTG	2 cut(s)	2972, 4179
CciI	TCATGA	4 cut(s)	1182, 2500, 3543, 4250
CfoI	GCGC	2 cut(s)	1584, 1863
Cfr10I	RCCGGY	2 cut(s)	2960, 4233
Cfr13I	GGNCC	4 cut(s)	48, 745, 3211, 3910
Cfr9I	CCCGGG	2 cut(s)	1065, 3846
CseI	GACGC	1 cut(s)	3278
Csp6I	GTAC	4 cut(s)	475, 1520, 2125, 3419
CspAI	ACCGGT	1 cut(s)	4233
CspCI	CAANNNNNGTGG	6 cut(s)	586, 621, 1824, 1859, 1865, 1900
CviQI	GTAC	4 cut(s)	475, 1520, 2125, 3419
DraI	TTTAAA	2 cut(s)	274, 1573
DriI	GACNNNNNGTC	2 cut(s)	1725, 4061
EaeI	YGGCCR	1 cut(s)	3264
EagI	CGGCCG	1 cut(s)	3264
Eam1104I	CTCTTC	4 cut(s)	1875, 2859, 3186, 3986
Eam1105I	GACNNNNNGTC	2 cut(s)	1725, 4061
EarI	CTCTTC	4 cut(s)	1875, 2859, 3186, 3986
Ecl136II	GAGCTC	2 cut(s)	645, 758
EclXI	CGGCCG	1 cut(s)	3264
Eco130I	CCWWGG	3 cut(s)	51, 4047, 4104
Eco147I	AGGCCT	1 cut(s)	694
Eco24I	GRGCYC	4 cut(s)	75, 647, 760, 2484
Eco31I	GGTCTC	3 cut(s)	15, 279, 523
Eco47I	GGWCC	3 cut(s)	745, 3211, 3910
Eco52I	CGGCCG	1 cut(s)	3264
Eco53kI	GAGCTC	2 cut(s)	645, 758
Eco57I	CTGAAG	5 cut(s)	522, 567, 1536, 2307, 3276
Eco81I	CCTNAGG	1 cut(s)	2268
Eco88I	CYCGRG	6 cut(s)	63, 76, 241, 1065, 3186, 3846
Eco91I	GGTNACC	2 cut(s)	55, 1790
EcoICRI	GAGCTC	2 cut(s)	645, 758
EcoO65I	GGTNACC	2 cut(s)	55, 1790
EcoRI	GAATTC	1 cut(s)	2117
EcoRII	CCWGG	4 cut(s)	510, 1017, 2731, 3751
EcoT14I	CCWWGG	3 cut(s)	51, 4047, 4104
EcoT22I	ATGCAT	3 cut(s)	675, 2578, 3733
EcoT38I	GRGCYC	4 cut(s)	75, 647, 760, 2484
ErhI	CCWWGG	3 cut(s)	51, 4047, 4104
Esp3I	CGTCTC	1 cut(s)	2409
FalI	AAGNNNNNCTT	4 cut(s)	183, 215, 4150, 4182
FaqI	GGGAC	4 cut(s)	881, 3224, 3274, 3634
FauI	CCCGC	1 cut(s)	3492
FauNDI	CATATG	1 cut(s)	4114
FbaI	TGATCA	4 cut(s)	436, 934, 1528, 1957
Fnu4HI	GCNGC	5 cut(s)	83, 86, 881, 930, 2082
FokI	GGATG	8 cut(s)	1136, 1283, 1826, 2126, 3002, 3611, 3779, 4087
FriOI	GRGCYC	4 cut(s)	75, 647, 760, 2484
Fsp4HI	GCNGC	5 cut(s)	83, 86, 881, 930, 2082
FspBI	CTAG	6 cut(s)	1491, 1838, 2802, 3537, 4194, 4203
FspI	TGCGCA	1 cut(s)	1583
GlaI	GCGC	2 cut(s)	1583, 1862
GluI	GCNGC	5 cut(s)	83, 86, 881, 930, 2082
GsuI	CTGGAG	2 cut(s)	2619, 4097
HaeIII	GGCC	7 cut(s)	49, 694, 1359, 2266, 3236, 3266, 4046
HapII	CCGG	6 cut(s)	1066, 1380, 2135, 2961, 3847, 4234
HgaI	GACGC	1 cut(s)	3278
HhaI	GCGC	2 cut(s)	1584, 1863
Hin1I	GRCGYC	2 cut(s)	2095, 3270
Hin6I	GCGC	2 cut(s)	1582, 1861
HinP1I	GCGC	2 cut(s)	1582, 1861
HincII	GTYRAC	3 cut(s)	235, 406, 730
HindII	GTYRAC	3 cut(s)	235, 406, 730
HindIII	AAGCTT	1 cut(s)	3665
HpaII	CCGG	6 cut(s)	1066, 1380, 2135, 2961, 3847, 4234
Hpy166II	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy8I	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy99I	CGWCG	2 cut(s)	23, 3272
HpyCH4IV	ACGT	2 cut(s)	2095, 3316
HpyF10VI	GCNNNNNNNGC	8 cut(s)	79, 787, 1449, 1838, 1867, 2018, 2081, 2582
HpySE526I	ACGT	2 cut(s)	2095, 3316
Hsp92I	GRCGYC	2 cut(s)	2095, 3270
HspAI	GCGC	2 cut(s)	1582, 1861
Kpn2I	TCCGGA	1 cut(s)	2134
Ksp22I	TGATCA	4 cut(s)	436, 934, 1528, 1957
LguI	GCTCTTC	1 cut(s)	3186
LmnI	GCTCC	7 cut(s)	613, 642, 1837, 2471, 2668, 2825, 3684
Lsp1109I	GCAGC	4 cut(s)	94, 892, 916, 2068
LweI	GCATC	7 cut(s)	121, 289, 952, 2585, 3040, 3064, 3512
MaeI	CTAG	6 cut(s)	1491, 1838, 2802, 3537, 4194, 4203
MaeII	ACGT	2 cut(s)	2095, 3316
MbiI	CCGCTC	1 cut(s)	4225
MfeI	CAATTG	2 cut(s)	938, 2201
MflI	RGATCY	4 cut(s)	1114, 1365, 2149, 3981
MhlI	GDGCHC	5 cut(s)	75, 647, 760, 2484, 3103
MlyI	GAGTC	7 cut(s)	412, 1661, 1735, 1982, 2876, 3377, 3616
MmeI	TCCRAC	1 cut(s)	3745
Mph1103I	ATGCAT	3 cut(s)	675, 2578, 3733
MroI	TCCGGA	1 cut(s)	2134
MroXI	GAANNNNTTC	6 cut(s)	195, 215, 2424, 3318, 3406, 4165
MslI	CAYNNNNRTG	2 cut(s)	2856, 3783
MspA1I	CMGCKG	1 cut(s)	3485
MspCI	CTTAAG	1 cut(s)	1999
MspI	CCGG	6 cut(s)	1066, 1380, 2135, 2961, 3847, 4234
MspR9I	CCNGG	8 cut(s)	512, 1019, 1066, 1067, 2733, 3753, 3847, 3848
MunI	CAATTG	2 cut(s)	938, 2201
Mva1269I	GAATGC	3 cut(s)	2424, 3133, 3553
MvaI	CCWGG	4 cut(s)	512, 1019, 2733, 3753
MvnI	CGCG	1 cut(s)	1861
MwoI	GCNNNNNNNGC	8 cut(s)	79, 787, 1449, 1838, 1867, 2018, 2081, 2582
NciI	CCSGG	4 cut(s)	1066, 1067, 3847, 3848
NcoI	CCATGG	2 cut(s)	51, 4047
NdeI	CATATG	1 cut(s)	4114
NheI	GCTAGC	1 cut(s)	1490
NlaIV	GGNNCC	4 cut(s)	1169, 1333, 3212, 3912
NmeAIII	GCCGAG	2 cut(s)	2242, 3262
NmuCI	GTSAC	5 cut(s)	14, 1790, 2377, 2849, 3695
NsbI	TGCGCA	1 cut(s)	1583
NsiI	ATGCAT	3 cut(s)	675, 2578, 3733
NspI	RCATGY	5 cut(s)	677, 880, 1582, 2523, 3353
NspV	TTCGAA	1 cut(s)	4169
PaeR7I	CTCGAG	2 cut(s)	63, 3186
PagI	TCATGA	4 cut(s)	1182, 2500, 3543, 4250
PaqCI	CACCTGC	1 cut(s)	3044
PceI	AGGCCT	1 cut(s)	694
PciI	ACATGT	2 cut(s)	2519, 3349
PciSI	GCTCTTC	1 cut(s)	3186
PcsI	WCGNNNNNNNCGW	2 cut(s)	3193, 3696
PctI	GAATGC	3 cut(s)	2424, 3133, 3553
PdmI	GAANNNNTTC	6 cut(s)	195, 215, 2424, 3318, 3406, 4165
PfeI	GAWTC	6 cut(s)	1762, 2795, 3161, 3407, 3527, 3540
PflFI	GACNNNGTC	1 cut(s)	3625
PflMI	CCANNNNNTGG	3 cut(s)	2249, 2789, 2990
PinAI	ACCGGT	1 cut(s)	4233
PkrI	GCNGC	5 cut(s)	84, 87, 882, 931, 2083
PleI	GAGTC	7 cut(s)	412, 1660, 1734, 1982, 2875, 3377, 3616
PpsI	GAGTC	7 cut(s)	412, 1660, 1734, 1982, 2875, 3377, 3616
PscI	ACATGT	2 cut(s)	2519, 3349
PshBI	ATTAAT	1 cut(s)	1410
PsiI	TTATAA	1 cut(s)	447
Psp124BI	GAGCTC	2 cut(s)	647, 760
Psp1406I	AACGTT	1 cut(s)	3316
Psp6I	CCWGG	4 cut(s)	510, 1017, 2731, 3751
PspEI	GGTNACC	2 cut(s)	55, 1790
PspGI	CCWGG	4 cut(s)	510, 1017, 2731, 3751
PspN4I	GGNNCC	4 cut(s)	1169, 1333, 3212, 3912
PspPI	GGNCC	4 cut(s)	48, 745, 3211, 3910
PsrI	GAACNNNNNNTAC	2 cut(s)	3306, 3338
PstNI	CAGNNNCTG	2 cut(s)	2972, 4179
PsuI	RGATCY	4 cut(s)	1114, 1365, 2149, 3981
PsyI	GACNNNGTC	1 cut(s)	3625
RsaI	GTAC	4 cut(s)	476, 1521, 2126, 3420
RsaNI	GTAC	4 cut(s)	475, 1520, 2125, 3419
RseI	CAYNNNNRTG	2 cut(s)	2856, 3783
SacI	GAGCTC	2 cut(s)	647, 760
SapI	GCTCTTC	1 cut(s)	3186
SatI	GCNGC	5 cut(s)	83, 86, 881, 930, 2082
Sau96I	GGNCC	4 cut(s)	48, 745, 3211, 3910
ScaI	AGTACT	1 cut(s)	1521
SchI	GAGTC	7 cut(s)	412, 1661, 1735, 1982, 2876, 3377, 3616
ScrFI	CCNGG	8 cut(s)	512, 1019, 1066, 1067, 2733, 3753, 3847, 3848
SduI	GDGCHC	5 cut(s)	75, 647, 760, 2484, 3103
SfaNI	GCATC	7 cut(s)	121, 289, 952, 2585, 3040, 3064, 3512
SfcI	CTRYAG	2 cut(s)	2580, 2950
Sfr274I	CTCGAG	2 cut(s)	63, 3186
SfuI	TTCGAA	1 cut(s)	4169
SinI	GGWCC	3 cut(s)	745, 3211, 3910
SlaI	CTCGAG	2 cut(s)	63, 3186
SmaI	CCCGGG	2 cut(s)	1067, 3848
SmiMI	CAYNNNNRTG	2 cut(s)	2856, 3783
SmlI	CTYRAG	6 cut(s)	63, 1999, 2237, 2759, 3186, 3385
SmoI	CTYRAG	6 cut(s)	63, 1999, 2237, 2759, 3186, 3385
SseBI	AGGCCT	1 cut(s)	694
SsiI	CCGC	7 cut(s)	86, 152, 2050, 3305, 3485, 3499, 4225
SspI	AATATT	3 cut(s)	294, 1394, 2171
SspMI	CTAG	6 cut(s)	1491, 1838, 2802, 3537, 4194, 4203
SstI	GAGCTC	2 cut(s)	647, 760
StuI	AGGCCT	1 cut(s)	694
StyD4I	CCNGG	8 cut(s)	510, 1017, 1064, 1065, 2731, 3751, 3845, 3846
StyI	CCWWGG	3 cut(s)	51, 4047, 4104
TaiI	ACGT	2 cut(s)	2098, 3319
TaqII	GACCGA	1 cut(s)	1736
TatI	WGTACW	3 cut(s)	474, 1519, 3418
TauI	GCSGC	1 cut(s)	88
TfiI	GAWTC	6 cut(s)	1762, 2795, 3161, 3407, 3527, 3540
TscAI	CASTG	6 cut(s)	730, 1233, 2250, 2749, 3700, 3955
TseFI	GTSAC	5 cut(s)	14, 1790, 2377, 2849, 3695
TseI	GCWGC	4 cut(s)	82, 880, 929, 2081
Tsp45I	GTSAC	5 cut(s)	14, 1790, 2377, 2849, 3695
TspGWI	ACGGA	2 cut(s)	913, 1724
TspMI	CCCGGG	2 cut(s)	1065, 3846
TspRI	CASTG	6 cut(s)	730, 1233, 2250, 2749, 3700, 3955
Tth111I	GACNNNGTC	1 cut(s)	3625
Van91I	CCANNNNNTGG	3 cut(s)	2249, 2789, 2990
Vha464I	CTTAAG	1 cut(s)	1999
VpaK11BI	GGWCC	3 cut(s)	745, 3211, 3910
VspI	ATTAAT	1 cut(s)	1410
XapI	RAATTY	7 cut(s)	380, 1136, 2110, 2117, 2383, 2398, 3590
XceI	RCATGY	5 cut(s)	677, 880, 1582, 2523, 3353
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2014, 3700
XhoI	CTCGAG	2 cut(s)	63, 3186
XmaI	CCCGGG	2 cut(s)	1065, 3846
XmnI	GAANNNNTTC	6 cut(s)	195, 215, 2424, 3318, 3406, 4165
XspI	CTAG	6 cut(s)	1491, 1838, 2802, 3537, 4194, 4203
ZraI	GACGTC	1 cut(s)	2096
ZrmI	AGTACT	1 cut(s)	1521
Zsp2I	ATGCAT	3 cut(s)	675, 2578, 3733

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.