Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002013

Physical Location & Seq

Genomic Coordinates Reverse (-)

71548149 .. 71555710

Gene Structure 7562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG417700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4326 bp

ATGAAGAGGTCTCGTGACGACGGTTATACAAGTTCGCAAGTTAAACGGCCCATGGTTACCTCTCGAGGCGAGCCCTCGGGGCAGCCGCAGATGATGACAGCAACAGCTTCCCAGAAACTAACAACCAATGATGCCTTATCATATCTAAAGGCGGTGAAGGAGATATTTGAAAACAACAAGGAAAAATACGAAGACTTTCTTGAAGTTATGAAAGACTTCAAAGCTCAGAGAGTTGACACCTCGGGTGTGATACAGAGGGTAAAGGATTTATTTAAAGGGCATCGAGACCTAATATTAGGCTTCAATACCTTCTTGCCGAAGGGATATGAAATCACTCTCCCTCTCGATGACGAACAACCTCCTCAAAAGAAGCCCGTTGAATTTGAAGAAGCTATCAGTTTTGTCAACAAAATTAAGACTCGATTTCAACATGATGATCATGTTTATAAATCCTTTTTGGACATTTTGAATATGTACAGAAAGGAAAACAAGTCCATCTCTGAAGTTTACCAGGAGGTCTCAGCACTTTTTCAAGACCACCCAGACTTACTTGTGGAGTTTACTCACTTTTTACCTGATACTTCAGCAACAGCCTCCACCCAGTTTGCTCCATCTCAAAGAAACTCAATGCTTCGTGATAGGAGCTCTGCCATGCCCCCTATGAGGCAAATGCATGTTGATAAGAAAGAAAGGCCTATGGGTTCTTACCCTGAGCATGATCTCAGTGTTGACCGTCCTGATCTGGACCATGATAGAGCTCTGATGAAAGCTGAGAAAGAGCAAAGGAGGCGTGGTGAAAAAGAAAAGGAGAGGAGAGAAGACAGAGAAAGACGAGATGATAGGGATTTTGACCATGATGGCAGTAGGGACTTCAACATGCAGCGTTTTCCCCACAAACGGAAATCCACTCGTAGGGGTGAAGACTTGGCTGCTGATCAATTGCATCAAGGTGGGGAAGACGATGAAAACCTTGGAGCACATCTCATTTCATCTTCTTATGATGATAAAAATTCTGCAAAAAGTATATATGGCTCGGAGAGTGCCTTCTGTGAGAAGGTGAAGGAGAAGTTACGAAATCCTGATGATTACCAGGAGTTTTTGAAGTGCCTCCATATTTATAGCAAGGAAATTATTACCCGGGCTGAATTGCAGTCATTGGTGGGTGATTTACTTGGAAAATATCCAGATCTTATGGATGGGTTCAATGAATTTTTGTCATGTTGTGAGAAGAAAGATGGGTTCCTTGCTGGTGTCATGAGTAAAAAATCTTTGTGGAATGAAGGCAATCTACCAAGACCAGTGAAGGTAGAGGATAAGGAGAAAGATCGAGATCGTGAAAGGGATGATGTGGTTAGAGATAGAGAGCGTGAAAACCGAGAAAGGGATAGACCTGATAGAAATGGTGCCTTTGGAAATAAAGAAATTGGAGGCCAGAAGATCTCTATATTTTCCGGCAAGGACAAATATTTGGCAAAACCTATTAATGAGCTTGACCTCTCTAATTGTGAGCGTTGCACTCCGAGCTATCGTCTTCTGCCAAAGAATTATCCAATACCTTCTGCTAGCCAAAGAACCGAACTTGGGTCTGAAGTACTAAATGATCATTGGGTGTCTGTTACTTCTGGAAGCGAGGATTATTCTTTTAAACACATGCGCAAAAACCAGTATGAAGAAAGCCTGTTTCGGTGTGAAGATGATAGGTTTGAATTAGATATGCTGTTAGAGTCTGTGAATGTAACAACCAAGCGTGTGGAAGAACTATTAGAGAAGATCAACAACAATACCATTAAGACCGAGAGTCCAATCCGTGTAGAAGAATACTTCACAGCTCTGAATCTAAGGTGTGTTGAGCGATTATATGGTGACCACGGGCTTGATGTAATGGATGTGTTGAGGAAAAATGCTCCACTAGCTTTGCCTGTTATATTAACGCGCTTGAAGCAAAAGCAAGAAGAGTGGGCAAGGTGTCGCTCTGATTTTAACAAAGTCTGGGCTGATATATATGCCAAGAACTACCACAAGTCGCTTGATCATCGTAGCTTCTACTTCAAGCAACAGGACTCAAAGAGCTTAAGCACCAAAGCCTTGTTGGCAGAGATTAAAGAGATGAGTGAGAAGAAGCGGAAGGAAGATGATGTTCTTCTTGCTATTGCTGCTGGAAATAGACGTCCTATTATTCCAAATTTGGAATTCGAGTACCCTGATCCGGACATCCATGAAGATCTGTATCAACTCATTAAATATTCTTGTGGAGAAGTTTGCACAACCGAGCAATTGGACAAAGTTATGAAGATATGGACAACCTTCCTTGAGCCAGTGCTTGGTGTTCCTCCTCGGCCTCAGGTTGCAGAGGATACTGAAGATGTTGTGAAGGCAAAAACTCATGCTGGCAAAAATGGTGCTGTGAGTGGGGGAGAGAGTGATGATAATCCTGATGGTGGGGCTACTGTGACAAATTCTAAACAGTTAAATTCATCGAGAAATGGAGACGAAAGCATTCAACCTGAACAATCAAGCTCTGCAAGAGCTTGGACAGTAAATGGAGCTAATGGGCTCAAAGAAGAAAGTTCTCATGATATTGATCGTGCTACATGTAAAGGTGATACTTTCTGCAATACCTCTCAACAAGGTAAAGTGCAAAGTAATGCATCTACAGCCGATGAAATGTCTGGAGTGAGCAAACAAGACAATTTTAATGAACGATTAGTCAATTCTAATGTATCACTTGCTACTGGCTTGGAGCAAAGCAATGGAAGAACTAATGTGGAAAACTCATCAGGTCTTAGTCCTACTCCTTCCAGACCTGGAAATGGCACTGTTGAGGGTGGGCTTGAGTTACCTTCACGGTCTTCACCAGAGGGTGGTGATTCTACTAGACCAGTTATATCCTCAAATGGAGCAATCACAGAAGCCACGAAAGGTCACAGATATGTGGAAGAGTCGGTTAGACACTTCAAGATTGAAAGAGAAGAAGGTGAAATATCTCCAAATGGAGATTTTGAGGAGGATAATTTTGCAAACTATAGAGAAGCCGGTTCAGAGGCTGTTCAAAAACCAAAGGATGGTGTTTCCAGCAGGCAACTTAAAGGCAGGCATGGGGAAGAAGAAATATGTGGTGGAGATGCAGGTGGAGAAAATGAGGCTGATGCTGATGATGAAGGTGAGGAAAGTGCTCATAGATCATCAGAGGATAGTGAGAATGCTTCTGAGAATGGCGATGTCTCTGGAAGTGAATCTGGTGAGGGAGAGGAATGTTCTCGAGAAGAGCGTGAGGAAGATGGGGACCATGATGAGCATGATACTAAGGCCGAGAGTGAAGGTGAAGCTGAAGGGACGGCCGACGCCCATGATGTTGAAGGAGATGGAACTTCTTTACCGCACTCAGAACGTTTTCTACTTTCTGTGAAGCCTCTTGCAAAACATGTTCTTCCAGCTTTACATGACAAGGACAAGGACTCAAGGATTTTCTATGGAAACGATTCTTTCTATGTACTTTTTAGGCTTCACCAAACACTATATGAGAGAATACAATCAGCAAAGATTAACTCGTCATCAGCGGAGAAGAAGTGGCGGGCATCAAATGATACAAGCACTTCTGATTCGTATGCTAGATTCATGAATGCCCTTTACAATTTACTTGACGGTTCTTCTGACAATACAAAATTTGAGGATGATTGTCGAGCTATCATAGGGACTCAGTCATATCTTCTTTTCACATTAGACAAGCTGATCTACAAGCTTGTTAAACAGCTCCAAACAGTCGCCAGTGACGAGATGGATAATAAACTTGTCCAACTATATGCATTTGAAAAATCAAGAAAACCAGGAAGATTTGTGGATGTAGTTTATCACGAAAATGCTCGTGTTCTTCTTCACGATGAGAACATTTATCGCATAGAATGTTTCTCTTTGCCAACCCGGGTGTCTATTCAGCTTATGGATTATGGGAATGATAAGCCTGAAATGACTGCTGTTTCAATGGACCCTAATTTTTCTGCTTATTTGCACAATGATTTTCTCACTGTTCTTCCTGACAAAAGAGAGAAGTCTGGGATCTTCTTGAAGAGGAACAAACGCAAATATGCGAGTTGTGATGATTTGTCTGCTATTTGTCAGGCCATGGAAGGACTTAAAGTCGCCAATGGTCTGGAGTGTAAGATAGCGTGTCATTCATCCAAGGTATCATATGTTTTAGATACAGAAGACTTTTTGTTTCGGACAAAAAGGAGAAGAAAAACTTCGAACAGTTCCTGCCATAACGAGGCTAGGTCTTCTAGTGGTTCAAGTAGAGTAGAGCGGTTTCACCGGTTGCTCTCTGGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1441

Amino Acids

163.47

Weight (kDa)

5.39

Isoelectric Point (pI)

45.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	61 - 105	3e-18	Paired amphipathic helix repeat
PAH	PF02671	147 - 191	2.3e-18	Paired amphipathic helix repeat
PAH	PF02671	362 - 405	1.6e-16	Paired amphipathic helix repeat
Sin3_corepress	PF08295	503 - 595	1.2e-36	Sin3 family co-repressor
Sin3a_C	PF16879	1156 - 1404	1.4e-48	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24190 AT1G24190 AT1G24190 AT1G70060 AT1G70060
fragaria_vesca	FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300
malus_domestica	MD13G1084700.v1.1 MD16G1083700.v1.1
prunus_persica	Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1
pyrus_communis	pycom13g07320 pycom16g07260
rosa_chinensis	RchiOBHm_Chr4g0444901
rosa_laevigata	RLG00000005790
rosa_multiflora	Rmu_sc0006845.1_g000002 Rmu_sc0013783.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385400
rosa_rugosa	Rorug04G0355800 Rorug04G0355900.1 Rorug04G0356000 Rorug04G0356100
rosa_samantha	Rh4AG417700 Rh4BG429500 Rh4CG444200 Rh4DG425400
rosa_wichuraiana	Rw4G035800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
AarI	CACCTGC	1 cut(s)	3116
AatII	GACGTC	1 cut(s)	2170
Acc16I	TGCGCA	1 cut(s)	1655
Acc36I	ACCTGC	1 cut(s)	3116
AccB1I	GGYRCC	1 cut(s)	1403
AccB7I	CCANNNNNTGG	3 cut(s)	2321, 2861, 3062
AccBSI	CCGCTC	1 cut(s)	4297
AccII	CGCG	1 cut(s)	1933
AccIII	TCCGGA	1 cut(s)	2206
AciI	CCGC	7 cut(s)	86, 152, 2122, 3377, 3557, 3571, 4297
AclI	AACGTT	1 cut(s)	3388
AclWI	GGATC	2 cut(s)	2198, 4061
AcoI	YGGCCR	1 cut(s)	3336
AcsI	RAATTY	8 cut(s)	380, 1009, 1208, 2182, 2189, 2455, 2470, 3662
AcuI	CTGAAG	5 cut(s)	522, 567, 1608, 2379, 3348
AcyI	GRCGYC	2 cut(s)	2167, 3342
AfaI	GTAC	4 cut(s)	476, 1593, 2198, 3492
AflII	CTTAAG	1 cut(s)	2071
AflIII	ACRYGT	2 cut(s)	2591, 3421
AgeI	ACCGGT	1 cut(s)	4305
AhdI	GACNNNNNGTC	2 cut(s)	1797, 4133
AjnI	CCWGG	4 cut(s)	510, 1089, 2803, 3823
Alw21I	GWGCWC	4 cut(s)	647, 760, 979, 3175
Alw26I	GTCTC	5 cut(s)	15, 279, 523, 2481, 3226
AlwI	GGATC	2 cut(s)	2198, 4061
AlwNI	CAGNNNCTG	2 cut(s)	3044, 4251
Ama87I	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
Aor13HI	TCCGGA	1 cut(s)	2206
AoxI	GGCC	7 cut(s)	47, 692, 1429, 2336, 3306, 3336, 4116
ApeKI	GCWGC	4 cut(s)	82, 880, 929, 2153
ApoI	RAATTY	8 cut(s)	380, 1009, 1208, 2182, 2189, 2455, 2470, 3662
AseI	ATTAAT	1 cut(s)	1482
AsiGI	ACCGGT	1 cut(s)	4305
Asp700I	GAANNNNTTC	6 cut(s)	195, 215, 2496, 3390, 3478, 4237
AspLEI	GCGC	2 cut(s)	1656, 1935
AspS9I	GGNCC	4 cut(s)	48, 745, 3283, 3982
AsuC2I	CCSGG	4 cut(s)	1138, 1139, 3919, 3920
AsuII	TTCGAA	1 cut(s)	4241
AsuNHI	GCTAGC	1 cut(s)	1562
AvaI	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
AvaII	GGWCC	3 cut(s)	745, 3283, 3982
AxyI	CCTNAGG	1 cut(s)	2340
BanI	GGYRCC	1 cut(s)	1403
BanII	GRGCYC	4 cut(s)	75, 647, 760, 2556
BarI	GAAGNNNNNNTAC	2 cut(s)	1272, 1304
BauI	CACGAG	2 cut(s)	12, 3861
BbsI	GAAGAC	8 cut(s)	198, 825, 927, 963, 1523, 2841, 4209, 4263
Bbv12I	GWGCWC	4 cut(s)	647, 760, 979, 3175
BbvI	GCAGC	4 cut(s)	94, 892, 916, 2140
BceAI	ACGGC	2 cut(s)	62, 3351
BciT130I	CCWGG	4 cut(s)	512, 1091, 2805, 3825
BciVI	GTATCC	1 cut(s)	2347
BclI	TGATCA	4 cut(s)	436, 934, 1600, 2029
BcnI	CCSGG	4 cut(s)	1138, 1139, 3919, 3920
BcoDI	GTCTC	5 cut(s)	15, 279, 523, 2481, 3226
BfaI	CTAG	6 cut(s)	1563, 1910, 2874, 3609, 4266, 4275
BfmI	CTRYAG	2 cut(s)	2652, 3022
BfrI	CTTAAG	1 cut(s)	2071
BfuAI	ACCTGC	1 cut(s)	3116
BfuI	GTATCC	1 cut(s)	2347
BglI	GCCNNNNNGGC	2 cut(s)	79, 2090
BglII	AGATCT	3 cut(s)	1186, 1437, 2221
BisI	GCNGC	5 cut(s)	83, 86, 881, 930, 2154
BlsI	GCNGC	5 cut(s)	84, 87, 882, 931, 2155
BmcAI	AGTACT	1 cut(s)	1593
Bme1390I	CCNGG	8 cut(s)	512, 1091, 1138, 1139, 2805, 3825, 3919, 3920
Bme18I	GGWCC	3 cut(s)	745, 3283, 3982
BmeRI	GACNNNNNGTC	2 cut(s)	1797, 4133
BmeT110I	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
BmgT120I	GGNCC	4 cut(s)	48, 745, 3283, 3982
BmiI	GGNNCC	4 cut(s)	1241, 1405, 3284, 3984
BmrFI	CCNGG	8 cut(s)	512, 1091, 1138, 1139, 2805, 3825, 3919, 3920
BmrI	ACTGGG	1 cut(s)	595
BmsI	GCATC	7 cut(s)	121, 289, 952, 2657, 3112, 3136, 3584
BmtI	GCTAGC	1 cut(s)	1566
BmuI	ACTGGG	1 cut(s)	595
BpiI	GAAGAC	8 cut(s)	198, 825, 927, 963, 1523, 2841, 4209, 4263
BplI	GAGNNNNNCTC	2 cut(s)	966, 998
BpmI	CTGGAG	2 cut(s)	2691, 4169
Bpu10I	CCTNAGC	1 cut(s)	711
Bpu14I	TTCGAA	1 cut(s)	4241
BpuEI	CTTGAG	3 cut(s)	2330, 2852, 3442
BpuMI	CCSGG	4 cut(s)	1138, 1139, 3919, 3920
BsaBI	GATNNNNATC	4 cut(s)	1329, 2226, 2427, 2580
BsaHI	GRCGYC	2 cut(s)	2167, 3342
BsaI	GGTCTC	3 cut(s)	15, 279, 523
BsaWI	WCCGGW	2 cut(s)	2206, 4305
BsaXI	ACNNNNNCTCC	4 cut(s)	778, 808, 3551, 3581
Bse118I	RCCGGY	2 cut(s)	3032, 4305
Bse1I	ACTGG	7 cut(s)	601, 1298, 1663, 2315, 2737, 2879, 3765
Bse21I	CCTNAGG	1 cut(s)	2340
Bse3DI	GCAATG	1 cut(s)	2755
Bse8I	GATNNNNATC	4 cut(s)	1329, 2226, 2427, 2580
BseAI	TCCGGA	1 cut(s)	2206
BseBI	CCWGG	4 cut(s)	512, 1091, 2805, 3825
BseGI	GGATG	8 cut(s)	1201, 1348, 1891, 2211, 3067, 3676, 3844, 4172
BseJI	GATNNNNATC	4 cut(s)	1329, 2226, 2427, 2580
BseMI	GCAATG	1 cut(s)	2755
BseMII	CTCAG	9 cut(s)	239, 534, 702, 736, 762, 2354, 3198, 3396, 3710
BseNI	ACTGG	7 cut(s)	601, 1298, 1663, 2315, 2737, 2879, 3765
BseRI	GAGGAG	4 cut(s)	351, 826, 2322, 3017
BseX3I	CGGCCG	1 cut(s)	3336
BseXI	GCAGC	4 cut(s)	94, 892, 916, 2140
Bsh1236I	CGCG	1 cut(s)	1933
Bsh1285I	CGRYCG	1 cut(s)	3339
BshFI	GGCC	7 cut(s)	49, 694, 1431, 2338, 3308, 3338, 4118
BshNI	GGYRCC	1 cut(s)	1403
BshTI	ACCGGT	1 cut(s)	4305
BsiEI	CGRYCG	1 cut(s)	3339
BsiHKAI	GWGCWC	4 cut(s)	647, 760, 979, 3175
BsiHKCI	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
BsiSI	CCGG	6 cut(s)	1138, 1452, 2207, 3033, 3919, 4306
BslFI	GGGAC	4 cut(s)	881, 3296, 3346, 3706
BsmAI	GTCTC	5 cut(s)	15, 279, 523, 2481, 3226
BsmBI	CGTCTC	1 cut(s)	2481
BsmFI	GGGAC	4 cut(s)	881, 3296, 3346, 3706
BsmI	GAATGC	3 cut(s)	2496, 3205, 3625
BsnI	GGCC	7 cut(s)	49, 694, 1431, 2338, 3308, 3338, 4118
Bso31I	GGTCTC	3 cut(s)	15, 279, 523
BsoBI	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
Bsp119I	TTCGAA	1 cut(s)	4241
Bsp1286I	GDGCHC	6 cut(s)	75, 647, 760, 979, 2556, 3175
Bsp13I	TCCGGA	1 cut(s)	2206
Bsp1407I	TGTACA	1 cut(s)	474
Bsp19I	CCATGG	2 cut(s)	51, 4119
BspACI	CCGC	7 cut(s)	86, 152, 2122, 3377, 3557, 3571, 4297
BspANI	GGCC	7 cut(s)	49, 694, 1431, 2338, 3308, 3338, 4118
BspCNI	CTCAG	9 cut(s)	238, 533, 703, 735, 763, 2353, 3199, 3395, 3709
BspEI	TCCGGA	1 cut(s)	2206
BspFNI	CGCG	1 cut(s)	1933
BspHI	TCATGA	4 cut(s)	1254, 2572, 3615, 4322
BspLI	GGNNCC	4 cut(s)	1241, 1405, 3284, 3984
BspMI	ACCTGC	1 cut(s)	3116
BspOI	GCTAGC	1 cut(s)	1566
BspPI	GGATC	2 cut(s)	2198, 4061
BspQI	GCTCTTC	1 cut(s)	3258
BspT104I	TTCGAA	1 cut(s)	4241
BspT107I	GGYRCC	1 cut(s)	1403
BspTI	CTTAAG	1 cut(s)	2071
BspTNI	GGTCTC	3 cut(s)	15, 279, 523
BsrBI	CCGCTC	1 cut(s)	4297
BsrDI	GCAATG	1 cut(s)	2755
BsrFI	RCCGGY	2 cut(s)	3032, 4305
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	7 cut(s)	601, 1298, 1663, 2315, 2737, 2879, 3765
BssAI	RCCGGY	2 cut(s)	3032, 4305
BssNI	GRCGYC	2 cut(s)	2167, 3342
BssSI	CACGAG	2 cut(s)	12, 3861
BssT1I	CCWWGG	4 cut(s)	51, 970, 4119, 4176
Bst2BI	CACGAG	2 cut(s)	12, 3861
Bst2UI	CCWGG	4 cut(s)	512, 1091, 2805, 3825
Bst6I	CTCTTC	4 cut(s)	1947, 2931, 3258, 4058
BstACI	GRCGYC	2 cut(s)	2167, 3342
BstAFI	CTTAAG	1 cut(s)	2071
BstAUI	TGTACA	1 cut(s)	474
BstBI	TTCGAA	1 cut(s)	4241
BstC8I	GCNNGC	6 cut(s)	71, 1564, 2389, 3077, 3092, 3573
BstDSI	CCRYGG	3 cut(s)	51, 1867, 4119
BstEII	GGTNACC	2 cut(s)	55, 1862
BstF5I	GGATG	8 cut(s)	1201, 1348, 1891, 2211, 3067, 3676, 3844, 4172
BstFNI	CGCG	1 cut(s)	1933
BstHHI	GCGC	2 cut(s)	1656, 1935
BstMAI	GTCTC	5 cut(s)	15, 279, 523, 2481, 3226
BstMCI	CGRYCG	1 cut(s)	3339
BstMWI	GCNNNNNNNGC	8 cut(s)	79, 787, 1521, 1910, 1939, 2090, 2153, 2654
BstNI	CCWGG	4 cut(s)	512, 1091, 2805, 3825
BstNSI	RCATGY	5 cut(s)	677, 880, 1654, 2595, 3425
BstPI	GGTNACC	2 cut(s)	55, 1862
BstSCI	CCNGG	8 cut(s)	510, 1089, 1136, 1137, 2803, 3823, 3917, 3918
BstSFI	CTRYAG	2 cut(s)	2652, 3022
BstUI	CGCG	1 cut(s)	1933
BstV1I	GCAGC	4 cut(s)	94, 892, 916, 2140
BstV2I	GAAGAC	8 cut(s)	198, 825, 927, 963, 1523, 2841, 4209, 4263
BstX2I	RGATCY	4 cut(s)	1186, 1437, 2221, 4053
BstXI	CCANNNNNNTGG	2 cut(s)	1750, 4147
BstYI	RGATCY	4 cut(s)	1186, 1437, 2221, 4053
BstZI	CGGCCG	1 cut(s)	3336
Bsu36I	CCTNAGG	1 cut(s)	2340
BsuI	GTATCC	1 cut(s)	2347
BsuRI	GGCC	7 cut(s)	49, 694, 1431, 2338, 3308, 3338, 4118
BtgI	CCRYGG	3 cut(s)	51, 1867, 4119
BtgZI	GCGATG	1 cut(s)	3231
BtsCI	GGATG	8 cut(s)	1201, 1348, 1891, 2211, 3067, 3676, 3844, 4172
BtsIMutI	CAGTG	6 cut(s)	730, 1305, 2322, 2814, 3772, 4020
BveI	ACCTGC	1 cut(s)	3116
Cac8I	GCNNGC	6 cut(s)	71, 1564, 2389, 3077, 3092, 3573
CaiI	CAGNNNCTG	2 cut(s)	3044, 4251
CciI	TCATGA	4 cut(s)	1254, 2572, 3615, 4322
CfoI	GCGC	2 cut(s)	1656, 1935
Cfr10I	RCCGGY	2 cut(s)	3032, 4305
Cfr13I	GGNCC	4 cut(s)	48, 745, 3283, 3982
Cfr9I	CCCGGG	2 cut(s)	1137, 3918
CseI	GACGC	1 cut(s)	3350
Csp6I	GTAC	4 cut(s)	475, 1592, 2197, 3491
CspAI	ACCGGT	1 cut(s)	4305
CspCI	CAANNNNNGTGG	6 cut(s)	586, 621, 1896, 1931, 1937, 1972
CviQI	GTAC	4 cut(s)	475, 1592, 2197, 3491
DraI	TTTAAA	2 cut(s)	274, 1645
DriI	GACNNNNNGTC	2 cut(s)	1797, 4133
EaeI	YGGCCR	1 cut(s)	3336
EagI	CGGCCG	1 cut(s)	3336
Eam1104I	CTCTTC	4 cut(s)	1947, 2931, 3258, 4058
Eam1105I	GACNNNNNGTC	2 cut(s)	1797, 4133
EarI	CTCTTC	4 cut(s)	1947, 2931, 3258, 4058
Ecl136II	GAGCTC	2 cut(s)	645, 758
EclXI	CGGCCG	1 cut(s)	3336
Eco130I	CCWWGG	4 cut(s)	51, 970, 4119, 4176
Eco147I	AGGCCT	1 cut(s)	694
Eco24I	GRGCYC	4 cut(s)	75, 647, 760, 2556
Eco31I	GGTCTC	3 cut(s)	15, 279, 523
Eco47I	GGWCC	3 cut(s)	745, 3283, 3982
Eco52I	CGGCCG	1 cut(s)	3336
Eco53kI	GAGCTC	2 cut(s)	645, 758
Eco57I	CTGAAG	5 cut(s)	522, 567, 1608, 2379, 3348
Eco81I	CCTNAGG	1 cut(s)	2340
Eco88I	CYCGRG	6 cut(s)	63, 76, 241, 1137, 3258, 3918
Eco91I	GGTNACC	2 cut(s)	55, 1862
EcoICRI	GAGCTC	2 cut(s)	645, 758
EcoO65I	GGTNACC	2 cut(s)	55, 1862
EcoRI	GAATTC	1 cut(s)	2189
EcoRII	CCWGG	4 cut(s)	510, 1089, 2803, 3823
EcoT14I	CCWWGG	4 cut(s)	51, 970, 4119, 4176
EcoT22I	ATGCAT	3 cut(s)	675, 2650, 3805
EcoT38I	GRGCYC	4 cut(s)	75, 647, 760, 2556
ErhI	CCWWGG	4 cut(s)	51, 970, 4119, 4176
Esp3I	CGTCTC	1 cut(s)	2481
FalI	AAGNNNNNCTT	4 cut(s)	183, 215, 4222, 4254
FaqI	GGGAC	4 cut(s)	881, 3296, 3346, 3706
FauI	CCCGC	1 cut(s)	3564
FauNDI	CATATG	1 cut(s)	4186
FbaI	TGATCA	4 cut(s)	436, 934, 1600, 2029
Fnu4HI	GCNGC	5 cut(s)	83, 86, 881, 930, 2154
FokI	GGATG	8 cut(s)	1208, 1355, 1898, 2198, 3074, 3683, 3851, 4159
FriOI	GRGCYC	4 cut(s)	75, 647, 760, 2556
Fsp4HI	GCNGC	5 cut(s)	83, 86, 881, 930, 2154
FspBI	CTAG	6 cut(s)	1563, 1910, 2874, 3609, 4266, 4275
FspI	TGCGCA	1 cut(s)	1655
GlaI	GCGC	2 cut(s)	1655, 1934
GluI	GCNGC	5 cut(s)	83, 86, 881, 930, 2154
GsuI	CTGGAG	2 cut(s)	2691, 4169
HaeIII	GGCC	7 cut(s)	49, 694, 1431, 2338, 3308, 3338, 4118
HapII	CCGG	6 cut(s)	1138, 1452, 2207, 3033, 3919, 4306
HgaI	GACGC	1 cut(s)	3350
HhaI	GCGC	2 cut(s)	1656, 1935
Hin1I	GRCGYC	2 cut(s)	2167, 3342
Hin6I	GCGC	2 cut(s)	1654, 1933
HinP1I	GCGC	2 cut(s)	1654, 1933
HincII	GTYRAC	3 cut(s)	235, 406, 730
HindII	GTYRAC	3 cut(s)	235, 406, 730
HindIII	AAGCTT	1 cut(s)	3737
HpaII	CCGG	6 cut(s)	1138, 1452, 2207, 3033, 3919, 4306
Hpy166II	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy8I	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy99I	CGWCG	2 cut(s)	23, 3344
HpyCH4IV	ACGT	2 cut(s)	2167, 3388
HpyF10VI	GCNNNNNNNGC	8 cut(s)	79, 787, 1521, 1910, 1939, 2090, 2153, 2654
HpySE526I	ACGT	2 cut(s)	2167, 3388
Hsp92I	GRCGYC	2 cut(s)	2167, 3342
HspAI	GCGC	2 cut(s)	1654, 1933
Kpn2I	TCCGGA	1 cut(s)	2206
Ksp22I	TGATCA	4 cut(s)	436, 934, 1600, 2029
LguI	GCTCTTC	1 cut(s)	3258
LmnI	GCTCC	8 cut(s)	613, 642, 974, 1909, 2543, 2740, 2897, 3756
Lsp1109I	GCAGC	4 cut(s)	94, 892, 916, 2140
LweI	GCATC	7 cut(s)	121, 289, 952, 2657, 3112, 3136, 3584
MaeI	CTAG	6 cut(s)	1563, 1910, 2874, 3609, 4266, 4275
MaeII	ACGT	2 cut(s)	2167, 3388
MbiI	CCGCTC	1 cut(s)	4297
MfeI	CAATTG	2 cut(s)	938, 2273
MflI	RGATCY	4 cut(s)	1186, 1437, 2221, 4053
MhlI	GDGCHC	6 cut(s)	75, 647, 760, 979, 2556, 3175
MlyI	GAGTC	7 cut(s)	412, 1733, 1807, 2054, 2948, 3449, 3688
MmeI	TCCRAC	1 cut(s)	3817
Mph1103I	ATGCAT	3 cut(s)	675, 2650, 3805
MroI	TCCGGA	1 cut(s)	2206
MroXI	GAANNNNTTC	6 cut(s)	195, 215, 2496, 3390, 3478, 4237
MslI	CAYNNNNRTG	3 cut(s)	948, 2928, 3855
MspA1I	CMGCKG	1 cut(s)	3557
MspCI	CTTAAG	1 cut(s)	2071
MspI	CCGG	6 cut(s)	1138, 1452, 2207, 3033, 3919, 4306
MspR9I	CCNGG	8 cut(s)	512, 1091, 1138, 1139, 2805, 3825, 3919, 3920
MunI	CAATTG	2 cut(s)	938, 2273
Mva1269I	GAATGC	3 cut(s)	2496, 3205, 3625
MvaI	CCWGG	4 cut(s)	512, 1091, 2805, 3825
MvnI	CGCG	1 cut(s)	1933
MwoI	GCNNNNNNNGC	8 cut(s)	79, 787, 1521, 1910, 1939, 2090, 2153, 2654
NciI	CCSGG	4 cut(s)	1138, 1139, 3919, 3920
NcoI	CCATGG	2 cut(s)	51, 4119
NdeI	CATATG	1 cut(s)	4186
NheI	GCTAGC	1 cut(s)	1562
NlaIV	GGNNCC	4 cut(s)	1241, 1405, 3284, 3984
NmeAIII	GCCGAG	2 cut(s)	2314, 3334
NmuCI	GTSAC	5 cut(s)	14, 1862, 2449, 2921, 3767
NsbI	TGCGCA	1 cut(s)	1655
NsiI	ATGCAT	3 cut(s)	675, 2650, 3805
NspI	RCATGY	5 cut(s)	677, 880, 1654, 2595, 3425
NspV	TTCGAA	1 cut(s)	4241
PaeR7I	CTCGAG	2 cut(s)	63, 3258
PagI	TCATGA	4 cut(s)	1254, 2572, 3615, 4322
PaqCI	CACCTGC	1 cut(s)	3116
PceI	AGGCCT	1 cut(s)	694
PciI	ACATGT	2 cut(s)	2591, 3421
PciSI	GCTCTTC	1 cut(s)	3258
PcsI	WCGNNNNNNNCGW	2 cut(s)	3265, 3768
PctI	GAATGC	3 cut(s)	2496, 3205, 3625
PdmI	GAANNNNTTC	6 cut(s)	195, 215, 2496, 3390, 3478, 4237
PfeI	GAWTC	6 cut(s)	1834, 2867, 3233, 3479, 3599, 3612
PflFI	GACNNNGTC	1 cut(s)	3697
PflMI	CCANNNNNTGG	3 cut(s)	2321, 2861, 3062
PinAI	ACCGGT	1 cut(s)	4305
PkrI	GCNGC	5 cut(s)	84, 87, 882, 931, 2155
PleI	GAGTC	7 cut(s)	412, 1732, 1806, 2054, 2947, 3449, 3688
PpsI	GAGTC	7 cut(s)	412, 1732, 1806, 2054, 2947, 3449, 3688
PscI	ACATGT	2 cut(s)	2591, 3421
PshBI	ATTAAT	1 cut(s)	1482
PsiI	TTATAA	1 cut(s)	447
Psp124BI	GAGCTC	2 cut(s)	647, 760
Psp1406I	AACGTT	1 cut(s)	3388
Psp6I	CCWGG	4 cut(s)	510, 1089, 2803, 3823
PspEI	GGTNACC	2 cut(s)	55, 1862
PspGI	CCWGG	4 cut(s)	510, 1089, 2803, 3823
PspN4I	GGNNCC	4 cut(s)	1241, 1405, 3284, 3984
PspPI	GGNCC	4 cut(s)	48, 745, 3283, 3982
PsrI	GAACNNNNNNTAC	2 cut(s)	3378, 3410
PstNI	CAGNNNCTG	2 cut(s)	3044, 4251
PsuI	RGATCY	4 cut(s)	1186, 1437, 2221, 4053
PsyI	GACNNNGTC	1 cut(s)	3697
RsaI	GTAC	4 cut(s)	476, 1593, 2198, 3492
RsaNI	GTAC	4 cut(s)	475, 1592, 2197, 3491
RseI	CAYNNNNRTG	3 cut(s)	948, 2928, 3855
SacI	GAGCTC	2 cut(s)	647, 760
SapI	GCTCTTC	1 cut(s)	3258
SatI	GCNGC	5 cut(s)	83, 86, 881, 930, 2154
Sau96I	GGNCC	4 cut(s)	48, 745, 3283, 3982
ScaI	AGTACT	1 cut(s)	1593
SchI	GAGTC	7 cut(s)	412, 1733, 1807, 2054, 2948, 3449, 3688
ScrFI	CCNGG	8 cut(s)	512, 1091, 1138, 1139, 2805, 3825, 3919, 3920
SduI	GDGCHC	6 cut(s)	75, 647, 760, 979, 2556, 3175
SfaNI	GCATC	7 cut(s)	121, 289, 952, 2657, 3112, 3136, 3584
SfcI	CTRYAG	2 cut(s)	2652, 3022
Sfr274I	CTCGAG	2 cut(s)	63, 3258
SfuI	TTCGAA	1 cut(s)	4241
SinI	GGWCC	3 cut(s)	745, 3283, 3982
SlaI	CTCGAG	2 cut(s)	63, 3258
SmaI	CCCGGG	2 cut(s)	1139, 3920
SmiMI	CAYNNNNRTG	3 cut(s)	948, 2928, 3855
SmlI	CTYRAG	6 cut(s)	63, 2071, 2309, 2831, 3258, 3457
SmoI	CTYRAG	6 cut(s)	63, 2071, 2309, 2831, 3258, 3457
SseBI	AGGCCT	1 cut(s)	694
SsiI	CCGC	7 cut(s)	86, 152, 2122, 3377, 3557, 3571, 4297
SspI	AATATT	3 cut(s)	294, 1466, 2243
SspMI	CTAG	6 cut(s)	1563, 1910, 2874, 3609, 4266, 4275
SstI	GAGCTC	2 cut(s)	647, 760
StuI	AGGCCT	1 cut(s)	694
StyD4I	CCNGG	8 cut(s)	510, 1089, 1136, 1137, 2803, 3823, 3917, 3918
StyI	CCWWGG	4 cut(s)	51, 970, 4119, 4176
TaiI	ACGT	2 cut(s)	2170, 3391
TaqII	GACCGA	1 cut(s)	1808
TatI	WGTACW	3 cut(s)	474, 1591, 3490
TauI	GCSGC	1 cut(s)	88
TfiI	GAWTC	6 cut(s)	1834, 2867, 3233, 3479, 3599, 3612
TscAI	CASTG	6 cut(s)	730, 1305, 2322, 2821, 3772, 4027
TseFI	GTSAC	5 cut(s)	14, 1862, 2449, 2921, 3767
TseI	GCWGC	4 cut(s)	82, 880, 929, 2153
Tsp45I	GTSAC	5 cut(s)	14, 1862, 2449, 2921, 3767
TspGWI	ACGGA	2 cut(s)	913, 1796
TspMI	CCCGGG	2 cut(s)	1137, 3918
TspRI	CASTG	6 cut(s)	730, 1305, 2322, 2821, 3772, 4027
Tth111I	GACNNNGTC	1 cut(s)	3697
Van91I	CCANNNNNTGG	3 cut(s)	2321, 2861, 3062
Vha464I	CTTAAG	1 cut(s)	2071
VpaK11BI	GGWCC	3 cut(s)	745, 3283, 3982
VspI	ATTAAT	1 cut(s)	1482
XapI	RAATTY	8 cut(s)	380, 1009, 1208, 2182, 2189, 2455, 2470, 3662
XceI	RCATGY	5 cut(s)	677, 880, 1654, 2595, 3425
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2086, 3772
XhoI	CTCGAG	2 cut(s)	63, 3258
XmaI	CCCGGG	2 cut(s)	1137, 3918
XmnI	GAANNNNTTC	6 cut(s)	195, 215, 2496, 3390, 3478, 4237
XspI	CTAG	6 cut(s)	1563, 1910, 2874, 3609, 4266, 4275
ZraI	GACGTC	1 cut(s)	2168
ZrmI	AGTACT	1 cut(s)	1593
Zsp2I	ATGCAT	3 cut(s)	675, 2650, 3805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.