Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002013

Physical Location & Seq

Genomic Coordinates Forward (+)

1777905 .. 1785647

Gene Structure 7743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005790

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3855 bp

ATGAAGAGGTCTCGTGACGACGGTTATACAAGCTCGCAAGTTAAACGGCCCATGGTTACCTCTCGAGGCGAGCCCTCGGGGCAGCCGCAGATGATTACAACAACAGCTTCCCAGAAACTAACAACCAATGATGCCTTATCATATCTAAAGGCGGTGAAGGAGATATTTGAAAACAACAAGGAAAAATACGAAGACTTTCTTGAAGTTATGAAAGACTTCAAAGCTCAGAGAGTTGACACCTCGGGTGTGATACAGAGGGTAAAGGATTTATTTAAAGGGCATCGAGACCTAATATTAGGCTTCAATACCTTCTTGCCGAAGGGATATGAAATCACTCTCCCTCTCGATGACGAACAACCTCCTCAAAAGAAGCCCGTTGAATTTGAAGAAGCTATCAGTTTTGTCAACAAAATTAAGACTCGATTTCAACATGATGATCATGTTTATAAATCCTTTTTGGACATTTTGAATATGTACAGAAAGGAAAACAAGTCCATCTCTGAAGTTTACCAGGAGGTCTCAGCACTTTTTCAAGATCACCCAGACTTACTTGTGGAGTTTACTCACTTTTTACCTGATACTTCAGCAACAGCCTCCACCCAGTTTGCTCCATCTCAAAGAAACTCAATGCTTCGTGATAGGAGCTCTGCCATGCCCCCTATGAGGCAAATGCATGTTGATAAGAAAGAAAGGCCTATGGGGTCGTACCCTGAGCATGATCTCAGTGTTGACCGTATATATGGCTCGGAGAGTGCCTTCTGTGAGAAGGTGAAGGAGAAGTTACGAAATCCTGATGATTACCAGGAGTTTTTGAAGTGCCTCCATATTTATAGCAAGGAAATTATTACCCGGGCTGAATTGCAGTCATTGGTGGGTGATTTACTTGGAAAATATCCGGATCTTATGGATGGGTTCAATGAATTTTTGTCATGTTGTGAGAAGAAAGATGGGTTCCTTGCTGGTGTCATGAGTAAAAAATCTTTGTGGAATGAAGGCAATCTACCAAGACCAGTGAAGGTAGAGGATAAGGAGAAAGATCGAGATCGTGAAAGGGATGATGCGGTTAGAGATAGAGAGCGTGAAAACCGAGAAAGGGATAGACCTGATAGAAATGGTGCCTTTGGAAATAAAGAGATTGGAGGCCAGAAGATCTCTATATTTTCTGGCAAGGACAAATATTTGTATGAAGAAAGCCTGTTTCGGTGTGAAGATGATAGGTTTGAATTAGATATGCTGTTAGAGTCTGTGAATGTAACGACCAAGCGTGTGGAAGAACTATTAGAGAAGATCAACAACAATACCATTAAGACTGAGAGTCCAATCCGTGTAGAAGAATACTTCACAGCTCTGAATCTAAGGTGCGTTGAGCGATTATATGGTGACCATGGGCTTGATGTAATGGATGTGTTGAGGAAAAATGCTCCACTAGCTTTGCCTGTTATATTAACGCGCTTGAAGCAAAAGCAAGAAGAGTGGGCAAGGTGTCGCTCTGATTTTAACAAAGTCTGGGCTGATATATATGCCAAGAACTATCACAAGTCACTTGATCATCGTAGCTTCTACTTCAAGCAACAGGACTCAAAGAGCTTAAGCACCAAAGCCTTGTTGGCAGAGATTAAAGAGATGAGTGAGAAGAAGCGGAAGGAAGATGATGTTCTTCTTGCTATTGCTGCTGGAAATAGACGTCCTATTATTCCAAATTTGGAATTCGAGTACCCTGATCCGGACATCCATGAAGATCTGTATCAACTCATCAAATATTCTTGTGGAGAAGTTTGCACAACTGAGCAATTGGACAAAGTTATGAAGATATGGACAACCTTCCTTGAGCCAGTGCTTGGTGTTCCTCCTCGGCCTCAGGTTGCAGAGGATACTGAAGATGTTGTGAAGGCGAAAACTCATGCTGGCAAAAATGGTGCTGTGAGTGGGGGAGAGAGTGATGATAATCCTGATGGTGGGGCTACTGTGACAAATTCTAAACAGTTAATTTCATCGAGAAATGGAGACGAAAGCATTCAACCTGAACAATCAAGCTCTGCAAGAGCTTGGACAGTAAATGGAGCTAATGGGCTCAAAGAAGAAAGTTCTCATGACATTGATCGTGCTACATGTAAAGGTGATACTTTCTGCAATACCTCTCAACAAGGTAAAGTGCAAAGTAATGCATCTACAGCCGATGAAATGTCTGGAGTTAGCAAACAAGACAATTTTAATGAACGATTAGTCAATTCTAATGTATCACTTGCTGCTGGCTTGGAGCAAAGCAATGGAAGAACTAATGTGGAAAACTCATCAGGCCTTAGTCCTGCTCCTTCCAGACCTGGAAATGGCACTGTTGAGGGTGGGCTTGAGTTACCTTCACGGTCTTCACCGGAGGGTGGTGATTCTACTAGACCAGTTATATCCTCAAATGGAGCAATTACAGAAGCCACGAAAGGTCACAGATATGTGGAAGAGTCGGTTAGACACTTCAAGATTGAAAGAGAAGAAGGTGAAATATCTCCAAATGGAGATTTTGAGGAGGATAATTTTGCAAACTATAGAGAAGCCGGTTCAGAGGCTGTTCAAAAACCAAAGGATGGTGTTTCCAGCAGGCAACTTAAAGGCAGACATGGGGAAGAAGAAATATGTGGTGGAGATGCAGGTGGAGAAAATGAGGCTGACGCTGATGATGAAGGTGAGGAAAGTGCTCATAGATCATCAGAGGATAGTGAGAATGCTTCCGAGAATGGCGATGTCTCTGGAAGTGAATCTGGTGAAGGAGAGGAATGTTCTCGAGAAGAGCGTGAGGAAGATGGGGACCATGATGAGCATGATACTAAGGCCGAGAGTGAAGGTGAAGCTGAAGGGACGGCCGACGCCCATGATGTTGAAGGAGATGGAACTTCTTTACCGCACTCAGAACGTTTTCTACTTTCTGTGAAGCCTCTTGCAAAACATGTTCTTCCAGCTTTACATGACAAGGACAAGGACTCAAGCATTTTCTATGGAAATGATTCTTTCTATGTACTTTTTAGGCTTCACCAAACACTGTATGAGAGAATACAATCAGCAAAGATTAACTCGTCATCAGCAGAGAAGAAGTGGCGGGCATCAAATGATACAAGCACTTCTGATTCGTATGCTAGATTCATGAATGCCCTTTACAATTTACTTGATGGTTCTTCTGACAATACAAAATTTGAGGATGATTGTCGAGCTATCATTGGGACTCAGTCATATCTTCTTTTCACATTAGACAAGCTGATCTACAAGCTTGTTAAACAGCTCCAAACAGTCGCCAGTGACGAGATGGATAATAAACTTGTCCAACTATATGCATTTGAAAAATCAAGAAAACCAGGAAGATTTGTGGATGTAGTTTATCACGAAAATGCTCGTGTTCTTCTGCATGATGAGAACATTTATCGCATAGAATGTTTCTCTTCGCCAACCCGGGTGTCTATTCAGCTTATGGATTATGGGAATGATAAGCCTGAAATGACTGCTGTTTCAATGGACCCTAATTTTTCTGCTTATCTGCACAATGATTTTCTCACTGTTCTTCCTGACAAAAGAGAGAAGTCTGGGATCTTCTTGAAGAGGAACAAACGCAAATATGCGAGTTGTGATGATTTGTCTGCTAGTTGTCAGGCCATGGAAGGACTTAAAGTCGCCAATGGTCTGGAGTGTAAGATAGCTTGTCATTCATCCAAGGTGTCATATGTTTTAGATACAGAAGACTTTTTGTTTCGGACAAAAAGGAGAAGAAAAACTTCAAACAGTTCCTGCCATAACCAGGCTAGTATATATGTAGCATTTTCAACGCATTGTATGCACCAGCGCTGGAGATTTGGAAAAATTGAGGCGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1285

Amino Acids

145.15

Weight (kDa)

5.28

Isoelectric Point (pI)

46.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	61 - 105	2.7e-18	Paired amphipathic helix repeat
PAH	PF02671	147 - 191	2e-18	Paired amphipathic helix repeat
PAH	PF02671	266 - 309	1.4e-16	Paired amphipathic helix repeat
Sin3_corepress	PF08295	387 - 434	6.9e-11	Sin3 family co-repressor
Sin3a_C	PF16879	995 - 1243	9.2e-50	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24190 AT1G24190 AT1G24190 AT1G70060 AT1G70060
fragaria_vesca	FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300
malus_domestica	MD13G1084700.v1.1 MD16G1083700.v1.1
prunus_persica	Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1
pyrus_communis	pycom13g07320 pycom16g07260
rosa_chinensis	RchiOBHm_Chr4g0444901
rosa_laevigata	RLG00000005790
rosa_multiflora	Rmu_sc0006845.1_g000002 Rmu_sc0013783.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385400
rosa_rugosa	Rorug04G0355800 Rorug04G0355900.1 Rorug04G0356000 Rorug04G0356100
rosa_samantha	Rh4AG417700 Rh4BG429500 Rh4CG444200 Rh4DG425400
rosa_wichuraiana	Rw4G035800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
AarI	CACCTGC	1 cut(s)	2633
AatII	GACGTC	1 cut(s)	1687
Acc36I	ACCTGC	1 cut(s)	2633
AccB1I	GGYRCC	1 cut(s)	1115
AccB7I	CCANNNNNTGG	2 cut(s)	1838, 2579
AccII	CGCG	1 cut(s)	1450
AccIII	TCCGGA	2 cut(s)	895, 1723
AciI	CCGC	6 cut(s)	86, 152, 1061, 1639, 2894, 3088
AclI	AACGTT	1 cut(s)	2905
AclWI	GGATC	3 cut(s)	906, 1715, 3578
AcoI	YGGCCR	1 cut(s)	2853
AcsI	RAATTY	6 cut(s)	380, 920, 1699, 1706, 1972, 3179
AcuI	CTGAAG	4 cut(s)	522, 567, 1896, 2865
AcyI	GRCGYC	2 cut(s)	1684, 2859
AfaI	GTAC	4 cut(s)	476, 707, 1715, 3009
AfeI	AGCGCT	1 cut(s)	3824
AfiI	CCNNNNNNNGG	9 cut(s)	663, 1093, 1723, 1838, 1955, 2327, 2378, 2579, 3778
AflII	CTTAAG	1 cut(s)	1588
AflIII	ACRYGT	2 cut(s)	2108, 2938
AhdI	GACNNNNNGTC	2 cut(s)	1314, 3650
AjnI	CCWGG	5 cut(s)	510, 801, 2320, 3340, 3777
Alw21I	GWGCWC	2 cut(s)	647, 2692
Alw26I	GTCTC	5 cut(s)	15, 279, 523, 1998, 2743
AlwI	GGATC	3 cut(s)	906, 1715, 3578
AlwNI	CAGNNNCTG	2 cut(s)	2561, 3768
Ama87I	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
Aor13HI	TCCGGA	2 cut(s)	895, 1723
Aor51HI	AGCGCT	1 cut(s)	3824
AoxI	GGCC	8 cut(s)	47, 692, 1141, 1853, 2296, 2823, 2853, 3633
ApeKI	GCWGC	3 cut(s)	82, 1670, 2246
ApoI	RAATTY	6 cut(s)	380, 920, 1699, 1706, 1972, 3179
Asp700I	GAANNNNTTC	6 cut(s)	195, 215, 2013, 2907, 2995, 3754
AspLEI	GCGC	3 cut(s)	1452, 3825, 3850
AspS9I	GGNCC	3 cut(s)	48, 2800, 3499
AsuC2I	CCSGG	4 cut(s)	850, 851, 3436, 3437
AvaI	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
AvaII	GGWCC	2 cut(s)	2800, 3499
AxyI	CCTNAGG	1 cut(s)	1857
BanI	GGYRCC	1 cut(s)	1115
BanII	GRGCYC	3 cut(s)	75, 647, 2073
BarI	GAAGNNNNNNTAC	2 cut(s)	984, 1016
BauI	CACGAG	2 cut(s)	12, 3378
BbsI	GAAGAC	3 cut(s)	198, 2358, 3726
Bbv12I	GWGCWC	2 cut(s)	647, 2692
BbvI	GCAGC	3 cut(s)	94, 1657, 2233
BceAI	ACGGC	2 cut(s)	62, 2868
BciT130I	CCWGG	5 cut(s)	512, 803, 2322, 3342, 3779
BciVI	GTATCC	1 cut(s)	1864
BclI	TGATCA	2 cut(s)	436, 1546
BcnI	CCSGG	4 cut(s)	850, 851, 3436, 3437
BcoDI	GTCTC	5 cut(s)	15, 279, 523, 1998, 2743
BfaI	CTAG	5 cut(s)	1427, 2391, 3126, 3624, 3783
BfmI	CTRYAG	2 cut(s)	2169, 2539
BfoI	RGCGCY	2 cut(s)	3826, 3851
BfrI	CTTAAG	1 cut(s)	1588
BfuAI	ACCTGC	1 cut(s)	2633
BfuI	GTATCC	1 cut(s)	1864
BglI	GCCNNNNNGGC	2 cut(s)	79, 1607
BglII	AGATCT	2 cut(s)	1149, 1738
BisI	GCNGC	4 cut(s)	83, 86, 1671, 2247
BlsI	GCNGC	4 cut(s)	84, 87, 1672, 2248
Bme1390I	CCNGG	9 cut(s)	512, 803, 850, 851, 2322, 3342, 3436, 3437, 3779
Bme18I	GGWCC	2 cut(s)	2800, 3499
BmeRI	GACNNNNNGTC	2 cut(s)	1314, 3650
BmeT110I	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
BmgT120I	GGNCC	3 cut(s)	48, 2800, 3499
BmiI	GGNNCC	4 cut(s)	953, 1117, 2801, 3501
BmrFI	CCNGG	9 cut(s)	512, 803, 850, 851, 2322, 3342, 3436, 3437, 3779
BmrI	ACTGGG	1 cut(s)	595
BmsI	GCATC	6 cut(s)	121, 289, 1048, 2174, 2629, 3101
BmuI	ACTGGG	1 cut(s)	595
BpiI	GAAGAC	3 cut(s)	198, 2358, 3726
BpmI	CTGGAG	3 cut(s)	2208, 3686, 3847
Bpu10I	CCTNAGC	1 cut(s)	711
BpuEI	CTTGAG	3 cut(s)	1847, 2369, 2959
BpuMI	CCSGG	4 cut(s)	850, 851, 3436, 3437
BsaBI	GATNNNNATC	3 cut(s)	1041, 1743, 1944
BsaHI	GRCGYC	2 cut(s)	1684, 2859
BsaI	GGTCTC	3 cut(s)	15, 279, 523
BsaJI	CCNNGG	9 cut(s)	51, 75, 240, 849, 1384, 1850, 3435, 3636, 3693
BsaWI	WCCGGW	3 cut(s)	895, 1723, 2371
Bsc4I	CCNNNNNNNGG	9 cut(s)	663, 1093, 1723, 1838, 1955, 2327, 2378, 2579, 3778
Bse118I	RCCGGY	1 cut(s)	2549
Bse1I	ACTGG	5 cut(s)	601, 1010, 1832, 2396, 3282
Bse21I	CCTNAGG	1 cut(s)	1857
Bse3DI	GCAATG	1 cut(s)	2272
Bse8I	GATNNNNATC	3 cut(s)	1041, 1743, 1944
BseAI	TCCGGA	2 cut(s)	895, 1723
BseBI	CCWGG	5 cut(s)	512, 803, 2322, 3342, 3779
BseDI	CCNNGG	9 cut(s)	51, 75, 240, 849, 1384, 1850, 3435, 3636, 3693
BseGI	GGATG	8 cut(s)	913, 1060, 1408, 1728, 2584, 3193, 3361, 3689
BseJI	GATNNNNATC	3 cut(s)	1041, 1743, 1944
BseLI	CCNNNNNNNGG	9 cut(s)	663, 1093, 1723, 1838, 1955, 2327, 2378, 2579, 3778
BseMI	GCAATG	1 cut(s)	2272
BseMII	CTCAG	9 cut(s)	239, 534, 702, 736, 1302, 1776, 1871, 2913, 3227
BseNI	ACTGG	5 cut(s)	601, 1010, 1832, 2396, 3282
BseRI	GAGGAG	3 cut(s)	351, 1839, 2534
BseX3I	CGGCCG	1 cut(s)	2853
BseXI	GCAGC	3 cut(s)	94, 1657, 2233
BsgI	GTGCAG	1 cut(s)	3506
Bsh1236I	CGCG	1 cut(s)	1450
Bsh1285I	CGRYCG	1 cut(s)	2856
BshFI	GGCC	8 cut(s)	49, 694, 1143, 1855, 2298, 2825, 2855, 3635
BshNI	GGYRCC	1 cut(s)	1115
BsiEI	CGRYCG	1 cut(s)	2856
BsiHKAI	GWGCWC	2 cut(s)	647, 2692
BsiHKCI	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
BsiSI	CCGG	6 cut(s)	850, 896, 1724, 2372, 2550, 3436
BslFI	GGGAC	3 cut(s)	2813, 2863, 3223
BslI	CCNNNNNNNGG	9 cut(s)	663, 1093, 1723, 1838, 1955, 2327, 2378, 2579, 3778
BsmAI	GTCTC	5 cut(s)	15, 279, 523, 1998, 2743
BsmBI	CGTCTC	1 cut(s)	1998
BsmFI	GGGAC	3 cut(s)	2813, 2863, 3223
BsmI	GAATGC	3 cut(s)	2013, 2722, 3142
BsnI	GGCC	8 cut(s)	49, 694, 1143, 1855, 2298, 2825, 2855, 3635
Bso31I	GGTCTC	3 cut(s)	15, 279, 523
BsoBI	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
Bsp1286I	GDGCHC	4 cut(s)	75, 647, 2073, 2692
Bsp13I	TCCGGA	2 cut(s)	895, 1723
Bsp1407I	TGTACA	1 cut(s)	474
Bsp19I	CCATGG	3 cut(s)	51, 1384, 3636
BspACI	CCGC	6 cut(s)	86, 152, 1061, 1639, 2894, 3088
BspANI	GGCC	8 cut(s)	49, 694, 1143, 1855, 2298, 2825, 2855, 3635
BspCNI	CTCAG	9 cut(s)	238, 533, 703, 735, 1303, 1777, 1870, 2912, 3226
BspEI	TCCGGA	2 cut(s)	895, 1723
BspFNI	CGCG	1 cut(s)	1450
BspHI	TCATGA	3 cut(s)	966, 2089, 3132
BspLI	GGNNCC	4 cut(s)	953, 1117, 2801, 3501
BspMI	ACCTGC	1 cut(s)	2633
BspPI	GGATC	3 cut(s)	906, 1715, 3578
BspQI	GCTCTTC	1 cut(s)	2775
BspT107I	GGYRCC	1 cut(s)	1115
BspTI	CTTAAG	1 cut(s)	1588
BspTNI	GGTCTC	3 cut(s)	15, 279, 523
BsrDI	GCAATG	1 cut(s)	2272
BsrFI	RCCGGY	1 cut(s)	2549
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	5 cut(s)	601, 1010, 1832, 2396, 3282
BssAI	RCCGGY	1 cut(s)	2549
BssECI	CCNNGG	9 cut(s)	51, 75, 240, 849, 1384, 1850, 3435, 3636, 3693
BssNI	GRCGYC	2 cut(s)	1684, 2859
BssSI	CACGAG	2 cut(s)	12, 3378
BssT1I	CCWWGG	4 cut(s)	51, 1384, 3636, 3693
Bst2BI	CACGAG	2 cut(s)	12, 3378
Bst2UI	CCWGG	5 cut(s)	512, 803, 2322, 3342, 3779
Bst6I	CTCTTC	5 cut(s)	1464, 2448, 2775, 3430, 3575
BstACI	GRCGYC	2 cut(s)	1684, 2859
BstAFI	CTTAAG	1 cut(s)	1588
BstAPI	GCANNNNNTGC	1 cut(s)	3814
BstAUI	TGTACA	1 cut(s)	474
BstC8I	GCNNGC	6 cut(s)	35, 71, 1906, 2251, 2594, 3090
BstDSI	CCRYGG	3 cut(s)	51, 1384, 3636
BstEII	GGTNACC	2 cut(s)	55, 1379
BstF5I	GGATG	8 cut(s)	913, 1060, 1408, 1728, 2584, 3193, 3361, 3689
BstFNI	CGCG	1 cut(s)	1450
BstH2I	RGCGCY	2 cut(s)	3826, 3851
BstHHI	GCGC	3 cut(s)	1452, 3825, 3850
BstMAI	GTCTC	5 cut(s)	15, 279, 523, 1998, 2743
BstMCI	CGRYCG	1 cut(s)	2856
BstMWI	GCNNNNNNNGC	7 cut(s)	79, 1427, 1456, 1607, 1670, 2171, 3814
BstNI	CCWGG	5 cut(s)	512, 803, 2322, 3342, 3779
BstNSI	RCATGY	3 cut(s)	677, 2112, 2942
BstPI	GGTNACC	2 cut(s)	55, 1379
BstSCI	CCNGG	9 cut(s)	510, 801, 848, 849, 2320, 3340, 3434, 3435, 3777
BstSFI	CTRYAG	2 cut(s)	2169, 2539
BstUI	CGCG	1 cut(s)	1450
BstV1I	GCAGC	3 cut(s)	94, 1657, 2233
BstV2I	GAAGAC	3 cut(s)	198, 2358, 3726
BstX2I	RGATCY	4 cut(s)	898, 1149, 1738, 3570
BstXI	CCANNNNNNTGG	2 cut(s)	1267, 3664
BstYI	RGATCY	4 cut(s)	898, 1149, 1738, 3570
BstZI	CGGCCG	1 cut(s)	2853
Bsu36I	CCTNAGG	1 cut(s)	1857
BsuI	GTATCC	1 cut(s)	1864
BsuRI	GGCC	8 cut(s)	49, 694, 1143, 1855, 2298, 2825, 2855, 3635
BtgI	CCRYGG	3 cut(s)	51, 1384, 3636
BtgZI	GCGATG	1 cut(s)	2748
BtsCI	GGATG	8 cut(s)	913, 1060, 1408, 1728, 2584, 3193, 3361, 3689
BtsIMutI	CAGTG	7 cut(s)	730, 1017, 1839, 2331, 3029, 3289, 3537
BveI	ACCTGC	1 cut(s)	2633
Cac8I	GCNNGC	6 cut(s)	35, 71, 1906, 2251, 2594, 3090
CaiI	CAGNNNCTG	2 cut(s)	2561, 3768
CciI	TCATGA	3 cut(s)	966, 2089, 3132
CfoI	GCGC	3 cut(s)	1452, 3825, 3850
Cfr10I	RCCGGY	1 cut(s)	2549
Cfr13I	GGNCC	3 cut(s)	48, 2800, 3499
Cfr9I	CCCGGG	2 cut(s)	849, 3435
CseI	GACGC	2 cut(s)	2672, 2867
Csp6I	GTAC	4 cut(s)	475, 706, 1714, 3008
CspCI	CAANNNNNGTGG	6 cut(s)	586, 621, 1413, 1448, 1454, 1489
CviQI	GTAC	4 cut(s)	475, 706, 1714, 3008
DraI	TTTAAA	1 cut(s)	274
DriI	GACNNNNNGTC	2 cut(s)	1314, 3650
EaeI	YGGCCR	1 cut(s)	2853
EagI	CGGCCG	1 cut(s)	2853
Eam1104I	CTCTTC	5 cut(s)	1464, 2448, 2775, 3430, 3575
Eam1105I	GACNNNNNGTC	2 cut(s)	1314, 3650
EarI	CTCTTC	5 cut(s)	1464, 2448, 2775, 3430, 3575
Ecl136II	GAGCTC	1 cut(s)	645
EclXI	CGGCCG	1 cut(s)	2853
Eco130I	CCWWGG	4 cut(s)	51, 1384, 3636, 3693
Eco147I	AGGCCT	2 cut(s)	694, 2298
Eco24I	GRGCYC	3 cut(s)	75, 647, 2073
Eco31I	GGTCTC	3 cut(s)	15, 279, 523
Eco47I	GGWCC	2 cut(s)	2800, 3499
Eco47III	AGCGCT	1 cut(s)	3824
Eco52I	CGGCCG	1 cut(s)	2853
Eco53kI	GAGCTC	1 cut(s)	645
Eco57I	CTGAAG	4 cut(s)	522, 567, 1896, 2865
Eco81I	CCTNAGG	1 cut(s)	1857
Eco88I	CYCGRG	6 cut(s)	63, 76, 241, 849, 2775, 3435
Eco91I	GGTNACC	2 cut(s)	55, 1379
EcoICRI	GAGCTC	1 cut(s)	645
EcoO65I	GGTNACC	2 cut(s)	55, 1379
EcoRI	GAATTC	1 cut(s)	1706
EcoRII	CCWGG	5 cut(s)	510, 801, 2320, 3340, 3777
EcoT14I	CCWWGG	4 cut(s)	51, 1384, 3636, 3693
EcoT22I	ATGCAT	3 cut(s)	675, 2167, 3322
EcoT38I	GRGCYC	3 cut(s)	75, 647, 2073
ErhI	CCWWGG	4 cut(s)	51, 1384, 3636, 3693
Esp3I	CGTCTC	1 cut(s)	1998
FalI	AAGNNNNNCTT	4 cut(s)	183, 215, 3739, 3771
FaqI	GGGAC	3 cut(s)	2813, 2863, 3223
FauI	CCCGC	1 cut(s)	3081
FauNDI	CATATG	1 cut(s)	3703
FbaI	TGATCA	2 cut(s)	436, 1546
Fnu4HI	GCNGC	4 cut(s)	83, 86, 1671, 2247
FokI	GGATG	8 cut(s)	920, 1067, 1415, 1715, 2591, 3200, 3368, 3676
FriOI	GRGCYC	3 cut(s)	75, 647, 2073
Fsp4HI	GCNGC	4 cut(s)	83, 86, 1671, 2247
FspBI	CTAG	5 cut(s)	1427, 2391, 3126, 3624, 3783
GlaI	GCGC	3 cut(s)	1451, 3824, 3849
GluI	GCNGC	4 cut(s)	83, 86, 1671, 2247
GsuI	CTGGAG	3 cut(s)	2208, 3686, 3847
HaeII	RGCGCY	2 cut(s)	3826, 3851
HaeIII	GGCC	8 cut(s)	49, 694, 1143, 1855, 2298, 2825, 2855, 3635
HapII	CCGG	6 cut(s)	850, 896, 1724, 2372, 2550, 3436
HgaI	GACGC	2 cut(s)	2672, 2867
HhaI	GCGC	3 cut(s)	1452, 3825, 3850
Hin1I	GRCGYC	2 cut(s)	1684, 2859
Hin6I	GCGC	3 cut(s)	1450, 3823, 3848
HinP1I	GCGC	3 cut(s)	1450, 3823, 3848
HincII	GTYRAC	3 cut(s)	235, 406, 730
HindII	GTYRAC	3 cut(s)	235, 406, 730
HindIII	AAGCTT	1 cut(s)	3254
HpaII	CCGG	6 cut(s)	850, 896, 1724, 2372, 2550, 3436
Hpy166II	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy8I	GTNNAC	5 cut(s)	235, 406, 508, 561, 730
Hpy99I	CGWCG	2 cut(s)	23, 2861
HpyCH4IV	ACGT	2 cut(s)	1684, 2905
HpyF10VI	GCNNNNNNNGC	7 cut(s)	79, 1427, 1456, 1607, 1670, 2171, 3814
HpySE526I	ACGT	2 cut(s)	1684, 2905
Hsp92I	GRCGYC	2 cut(s)	1684, 2859
HspAI	GCGC	3 cut(s)	1450, 3823, 3848
Kpn2I	TCCGGA	2 cut(s)	895, 1723
Ksp22I	TGATCA	2 cut(s)	436, 1546
LguI	GCTCTTC	1 cut(s)	2775
LmnI	GCTCC	8 cut(s)	613, 642, 1426, 2060, 2257, 2314, 2414, 3273
Lsp1109I	GCAGC	3 cut(s)	94, 1657, 2233
LweI	GCATC	6 cut(s)	121, 289, 1048, 2174, 2629, 3101
MaeI	CTAG	5 cut(s)	1427, 2391, 3126, 3624, 3783
MaeII	ACGT	2 cut(s)	1684, 2905
MfeI	CAATTG	1 cut(s)	1790
MflI	RGATCY	4 cut(s)	898, 1149, 1738, 3570
MhlI	GDGCHC	4 cut(s)	75, 647, 2073, 2692
MlyI	GAGTC	7 cut(s)	412, 1250, 1324, 1571, 2465, 2966, 3205
MmeI	TCCRAC	1 cut(s)	3334
Mph1103I	ATGCAT	3 cut(s)	675, 2167, 3322
MroI	TCCGGA	2 cut(s)	895, 1723
MroXI	GAANNNNTTC	6 cut(s)	195, 215, 2013, 2907, 2995, 3754
MslI	CAYNNNNRTG	2 cut(s)	2445, 3372
MspCI	CTTAAG	1 cut(s)	1588
MspI	CCGG	6 cut(s)	850, 896, 1724, 2372, 2550, 3436
MspR9I	CCNGG	9 cut(s)	512, 803, 850, 851, 2322, 3342, 3436, 3437, 3779
MunI	CAATTG	1 cut(s)	1790
Mva1269I	GAATGC	3 cut(s)	2013, 2722, 3142
MvaI	CCWGG	5 cut(s)	512, 803, 2322, 3342, 3779
MvnI	CGCG	1 cut(s)	1450
MwoI	GCNNNNNNNGC	7 cut(s)	79, 1427, 1456, 1607, 1670, 2171, 3814
NciI	CCSGG	4 cut(s)	850, 851, 3436, 3437
NcoI	CCATGG	3 cut(s)	51, 1384, 3636
NdeI	CATATG	1 cut(s)	3703
NlaIV	GGNNCC	4 cut(s)	953, 1117, 2801, 3501
NmeAIII	GCCGAG	2 cut(s)	1831, 2851
NmuCI	GTSAC	6 cut(s)	14, 1379, 1539, 1966, 2438, 3284
NsiI	ATGCAT	3 cut(s)	675, 2167, 3322
NspI	RCATGY	3 cut(s)	677, 2112, 2942
PaeR7I	CTCGAG	2 cut(s)	63, 2775
PagI	TCATGA	3 cut(s)	966, 2089, 3132
PaqCI	CACCTGC	1 cut(s)	2633
PceI	AGGCCT	2 cut(s)	694, 2298
PciI	ACATGT	2 cut(s)	2108, 2938
PciSI	GCTCTTC	1 cut(s)	2775
PcsI	WCGNNNNNNNCGW	2 cut(s)	2782, 3285
PctI	GAATGC	3 cut(s)	2013, 2722, 3142
PdmI	GAANNNNTTC	6 cut(s)	195, 215, 2013, 2907, 2995, 3754
PfeI	GAWTC	6 cut(s)	1351, 2384, 2750, 2996, 3116, 3129
PflFI	GACNNNGTC	1 cut(s)	3214
PflMI	CCANNNNNTGG	2 cut(s)	1838, 2579
PkrI	GCNGC	4 cut(s)	84, 87, 1672, 2248
PleI	GAGTC	7 cut(s)	412, 1249, 1323, 1571, 2464, 2966, 3205
PpsI	GAGTC	7 cut(s)	412, 1249, 1323, 1571, 2464, 2966, 3205
PscI	ACATGT	2 cut(s)	2108, 2938
PsiI	TTATAA	1 cut(s)	447
Psp124BI	GAGCTC	1 cut(s)	647
Psp1406I	AACGTT	1 cut(s)	2905
Psp6I	CCWGG	5 cut(s)	510, 801, 2320, 3340, 3777
PspEI	GGTNACC	2 cut(s)	55, 1379
PspGI	CCWGG	5 cut(s)	510, 801, 2320, 3340, 3777
PspN4I	GGNNCC	4 cut(s)	953, 1117, 2801, 3501
PspPI	GGNCC	3 cut(s)	48, 2800, 3499
PsrI	GAACNNNNNNTAC	2 cut(s)	2895, 2927
PstNI	CAGNNNCTG	2 cut(s)	2561, 3768
PsuI	RGATCY	4 cut(s)	898, 1149, 1738, 3570
PsyI	GACNNNGTC	1 cut(s)	3214
RsaI	GTAC	4 cut(s)	476, 707, 1715, 3009
RsaNI	GTAC	4 cut(s)	475, 706, 1714, 3008
RseI	CAYNNNNRTG	2 cut(s)	2445, 3372
SacI	GAGCTC	1 cut(s)	647
SapI	GCTCTTC	1 cut(s)	2775
SatI	GCNGC	4 cut(s)	83, 86, 1671, 2247
Sau96I	GGNCC	3 cut(s)	48, 2800, 3499
SchI	GAGTC	7 cut(s)	412, 1250, 1324, 1571, 2465, 2966, 3205
ScrFI	CCNGG	9 cut(s)	512, 803, 850, 851, 2322, 3342, 3436, 3437, 3779
SduI	GDGCHC	4 cut(s)	75, 647, 2073, 2692
SfaNI	GCATC	6 cut(s)	121, 289, 1048, 2174, 2629, 3101
SfcI	CTRYAG	2 cut(s)	2169, 2539
Sfr274I	CTCGAG	2 cut(s)	63, 2775
SinI	GGWCC	2 cut(s)	2800, 3499
SlaI	CTCGAG	2 cut(s)	63, 2775
SmaI	CCCGGG	2 cut(s)	851, 3437
SmiMI	CAYNNNNRTG	2 cut(s)	2445, 3372
SmlI	CTYRAG	6 cut(s)	63, 1588, 1826, 2348, 2775, 2974
SmoI	CTYRAG	6 cut(s)	63, 1588, 1826, 2348, 2775, 2974
SseBI	AGGCCT	2 cut(s)	694, 2298
SsiI	CCGC	6 cut(s)	86, 152, 1061, 1639, 2894, 3088
SspI	AATATT	3 cut(s)	294, 1178, 1760
SspMI	CTAG	5 cut(s)	1427, 2391, 3126, 3624, 3783
SstI	GAGCTC	1 cut(s)	647
StuI	AGGCCT	2 cut(s)	694, 2298
StyD4I	CCNGG	9 cut(s)	510, 801, 848, 849, 2320, 3340, 3434, 3435, 3777
StyI	CCWWGG	4 cut(s)	51, 1384, 3636, 3693
TaiI	ACGT	2 cut(s)	1687, 2908
TaqI	TCGA	9 cut(s)	64, 283, 345, 421, 1039, 1710, 1994, 2776, 3196
TatI	WGTACW	2 cut(s)	474, 3007
TauI	GCSGC	1 cut(s)	88
TfiI	GAWTC	6 cut(s)	1351, 2384, 2750, 2996, 3116, 3129
TscAI	CASTG	7 cut(s)	730, 1017, 1839, 2338, 3036, 3289, 3544
TseFI	GTSAC	6 cut(s)	14, 1379, 1539, 1966, 2438, 3284
TseI	GCWGC	3 cut(s)	82, 1670, 2246
Tsp45I	GTSAC	6 cut(s)	14, 1379, 1539, 1966, 2438, 3284
TspGWI	ACGGA	1 cut(s)	1313
TspMI	CCCGGG	2 cut(s)	849, 3435
TspRI	CASTG	7 cut(s)	730, 1017, 1839, 2338, 3036, 3289, 3544
Tth111I	GACNNNGTC	1 cut(s)	3214
Van91I	CCANNNNNTGG	2 cut(s)	1838, 2579
Vha464I	CTTAAG	1 cut(s)	1588
VpaK11BI	GGWCC	2 cut(s)	2800, 3499
XapI	RAATTY	6 cut(s)	380, 920, 1699, 1706, 1972, 3179
XceI	RCATGY	3 cut(s)	677, 2112, 2942
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1603, 3289
XhoI	CTCGAG	2 cut(s)	63, 2775
XmaI	CCCGGG	2 cut(s)	849, 3435
XmnI	GAANNNNTTC	6 cut(s)	195, 215, 2013, 2907, 2995, 3754
XspI	CTAG	5 cut(s)	1427, 2391, 3126, 3624, 3783
ZraI	GACGTC	1 cut(s)	1685
Zsp2I	ATGCAT	3 cut(s)	675, 2167, 3322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.