Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0002013

Physical Location & Seq

Genomic Coordinates Reverse (-)

52856104 .. 52861151

Gene Structure 5048 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0356100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2520 bp

ATGTCCACCGTCGGCGGTGGCCGGAGTGAGGTTGAAGTAAAGCGAGTGTTCATAGGAGCAGGGTGCAACAGAGTAGTGAACAACGTCTCATGGGGTGCTTGTGATTTAGTCGCGTTCGGCGCCCAAAACGCCGTCGCTATTTTCGACCCAAAGACTGCTCAAATTTCGACTACGCTTCCGGGTCACAAGGCTTCTGTTAATTGCACCCAATGGCTTCCAAGTAATAAGTTTGCATTCAAAGCTAAAGGCTTGGACCGACATTATTTGCTCTCTGGAGATGCTGGGGGTGCCATTATTTTGTGGGAGTATTCTGCTCTTGAAGGAAAGTGGAGATATGTATTGCAAATACCTGAATTACACAAGAAGGGTGTCACATGCATTAGTGGAATTATGGTTTCTGAAACTGAGGCAGTCTTTGCGTCTACTTCATCGGATGGTACAGTTTATTTATGGGAGGTTGTTTTCCCATCTACTGGTGAAGGTGACTGTAAAATATTGCATTTGGATTCTCTATTTGTTGGTTCAAAACCAATGGTAGCACTTTCACTAGCAGAGTTGCCTGGGAATGCTGGGCATTTAGTCCTGGCCATGGGGGGATTGGATAACAAGATCCACCTATACTCTGGGGAGAGGAGAGGAAAGTTTGTTCGAGCCTGCGAGTTGAAAGGGCATACAGATTGGATCAGAAGTCTGGATTTCTCATTACCTATATGCACTGATGGTGAGGCACATAGTATTCTACTTGTTAGTTCATCACAAGACAAAGGCATACGCATATGGAAGATGGCTTTACGGGGTTCTTTGGACAGCAGCCAGAGTTCCAGGCAAGGAAAAATAAGCTTAGCATCTTACATAGAAGGCCCTGTACTTGTAGCTGGAACAACCTCGTATCAGATTTCACTGGAATCTCTTCTAATTGGTCATGAGGATTGGGTGTATTCAGTGGAGTGGCAACCTCCGTCACCTGTATCTTCAGAAGGGATTACCTATTGTCAACACCAGAGCATCTTATCTGCATCTATGGATAAGACAATGATGATCTGGAAACCAGAAAAAACTTCTGGTATCTGGATGAATGTTGTTACTGTTGGGGAATTAAGTCATTGTGCTCTAGGATTTTATGGTGGCCACTGGAGTCCCAGTGGAGATTCAATCTTAGCACATGGGTATGGTGGATCTTTTCATCTGTGGAGAAATGTCGGTACTAGCTTAGATAATTGGCAACCACAAAAAGTTCCATCTGGGCATTTTGCAGCTATTACTGATATTGCTTGGGGGAGATCTGGTGAATACTTGCTGTCAGTCAGTGATGACCAGACGACACGAATTTTTTCTCCGTGGCAAAATGAAACATCTGTAGGAGATGAGGGTTCTTGGCATGAAATTGCTCGTCCTCAAGTTCATGGTCATGATATGAACTGTGTCACCATCATCCAAGGAAAAGGAAACCATCGTTTTGTCAGTGGAGCCGATGAGAAAGTTGCCAGAGTGTTTGAAGCTCCTTTGTCTTTCTTGAAGACATTGGGTCATGCCATTTCACAAAACTCTACCTTTTCCGAAGATATGCAATTGGGTGTACAGATTTTGGGTGCAAATATGTCAGCTCTTGGGCTATCACAGAAACCTATTTATGTTCATGCTGAGCAGCACACCATAGAGAAGAATCCAAATGATAGCCTTGACACCCTTGAAGCCATTCCTGATGCAGTTCCTGTTGTGTTGACTGAACCTCCGATCGAAGATCAATTGGGATGGCATACACTATGGCCAGAATCACACAAGCTTTATGGTCATGGGAATGAGCTGTTTGCTCTTTGCAGTGATCATGAAGGGAAGCTTGTTGCTTCGTCATGTAAGGCCCAATCAGCAGCAGTCGCAGAAATATGGCTGTGGCAAGTTGGTTCCTGGAAAGCAGTTGGTCACTTGCAGTCTCATAGCTTGACTGTTACACAAATGGAATTCTCTCTTGATGACAAATTCCTGTTGGCTGTATCTAGGGATCGCCAGTTCTCTGTATTTTCAATTGACAAAACAGGCACTGATGAAACTAGTTACAAGCTGGTAGCAAAGCACGAGGCACACAAAAGAATCATATGGTCATGTTCCTGGAATCCCCGCAGCTATGAATTTGCAACAGGCTCGAGGGACAAGACAGTGAAGATCTGGACTTTTGGAAAAGATTCATCAGTTAAGTTGCTCGCGACTCTCCCACAGTTCAGCAGTAGCGTCACAGCACTATCGTGGGCTAGTCTTGATTCCAAGAAAAATAATGGATTGCTTGCAGTTGGAATGGAAAACGGACTCATTGAATTGTGGAGTCTCTCTGTTAACAGAACCGACGATGGTCTAGCAGCAGGTGTACTTGCAGCTCTCGTTGTACGGTTTGATCCATTGATGTGCCATGTTTCTTCTGTGAGCCGTTTGGCGTGGAGAAAGCGCAGGTATGAAGATTGCAGTAGCATACAGCTTGCTTCTTGTGGAGCTGATCATTGTGTGAGAGTGTTCGAGGTAAAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

839

Amino Acids

91.83

Weight (kDa)

6.2

Isoelectric Point (pI)

39.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	23 - 153	2.3e-06	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	23 - 153	3.2e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	37 - 153	7.9e-07	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	37 - 153	4.8e-07	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	86 - 351	1.3e-10	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	194 - 301	3.4e-08	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	194 - 299	8.5e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	304 - 500	4.5e-07	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	378 - 497	1.4e-06	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	588 - 767	7.8e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	594 - 723	3.5e-11	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	597 - 779	2.1e-16	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	621 - 725	1.3e-06	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	626 - 775	2.6e-11	CDC20/Fizzy WD40 domain
WD40	PF00400	686 - 722	4.2e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	694 - 776	1.8e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24190 AT1G24190 AT1G24190 AT1G70060 AT1G70060
fragaria_vesca	FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300 FvH4_4g35300
malus_domestica	MD13G1084700.v1.1 MD16G1083700.v1.1
prunus_persica	Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1 Prupe.1G262300_v2.0.a1
pyrus_communis	pycom13g07320 pycom16g07260
rosa_chinensis	RchiOBHm_Chr4g0444901
rosa_laevigata	RLG00000005790
rosa_multiflora	Rmu_sc0006845.1_g000002 Rmu_sc0013783.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385400
rosa_rugosa	Rorug04G0355800 Rorug04G0355900.1 Rorug04G0356000 Rorug04G0356100
rosa_samantha	Rh4AG417700 Rh4BG429500 Rh4CG444200 Rh4DG425400
rosa_wichuraiana	Rw4G035800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2345
Acc36I	ACCTGC	2 cut(s)	2345, 2430
AccB1I	GGYRCC	2 cut(s)	119, 287
AccB7I	CCANNNNNTGG	1 cut(s)	473
AccI	GTMKAC	1 cut(s)	422
AccII	CGCG	2 cut(s)	113, 2201
AciI	CCGC	2 cut(s)	15, 2116
AclWI	GGATC	5 cut(s)	604, 689, 1183, 2007, 2381
AcoI	YGGCCR	4 cut(s)	19, 585, 1126, 1766
AcsI	RAATTY	5 cut(s)	162, 1326, 1958, 1976, 2126
AcuI	CTGAAG	1 cut(s)	957
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	6 cut(s)	439, 867, 1204, 1578, 2361, 2379
AfiI	CCNNNNNNNGG	3 cut(s)	28, 473, 589
AhdI	GACNNNNNGTC	1 cut(s)	1524
AhlI	ACTAGT	1 cut(s)	2048
AjnI	CCWGG	5 cut(s)	559, 582, 821, 1904, 2105
AleI	CACNNNNGTG	1 cut(s)	2239
AloI	GAACNNNNNNTCC	4 cut(s)	630, 662, 1991, 2023
Alw21I	GWGCWC	1 cut(s)	1111
Alw26I	GTCTC	3 cut(s)	91, 1935, 2324
AlwI	GGATC	5 cut(s)	604, 689, 1183, 2007, 2381
AlwNI	CAGNNNCTG	2 cut(s)	1712, 2039
Ama87I	CYCGRG	1 cut(s)	2140
AoxI	GGCC	6 cut(s)	19, 585, 859, 1126, 1766, 1857
ApeKI	GCWGC	7 cut(s)	810, 1253, 1645, 1868, 2118, 2351, 2366
ApoI	RAATTY	5 cut(s)	162, 1326, 1958, 1976, 2126
Asp700I	GAANNNNTTC	4 cut(s)	909, 1330, 1695, 2179
AspLEI	GCGC	2 cut(s)	122, 2439
AspS9I	GGNCC	3 cut(s)	253, 860, 1858
AsuC2I	CCSGG	1 cut(s)	180
AsuHPI	GGTGA	6 cut(s)	488, 494, 734, 954, 1298, 1417
AvaI	CYCGRG	1 cut(s)	2140
AvaII	GGWCC	1 cut(s)	253
BalI	TGGCCA	3 cut(s)	587, 1128, 1768
BanI	GGYRCC	2 cut(s)	119, 287
BarI	GAAGNNNNNNTAC	2 cut(s)	849, 881
BauI	CACGAG	1 cut(s)	2072
BbsI	GAAGAC	1 cut(s)	1523
Bbv12I	GWGCWC	1 cut(s)	1111
BbvI	GCAGC	7 cut(s)	822, 1265, 1657, 1880, 2130, 2363, 2378
BccI	CCATC	9 cut(s)	428, 475, 713, 778, 1246, 1436, 1458, 1746, 2336
BceAI	ACGGC	2 cut(s)	116, 2403
BcgI	CGANNNNNNTGC	2 cut(s)	2121, 2155
BciT130I	CCWGG	5 cut(s)	561, 584, 823, 1906, 2107
BclI	TGATCA	2 cut(s)	1822, 2485
BcnI	CCSGG	1 cut(s)	180
BcoDI	GTCTC	3 cut(s)	91, 1935, 2324
BcuI	ACTAGT	1 cut(s)	2048
BfaI	CTAG	7 cut(s)	548, 1112, 1206, 1995, 2049, 2247, 2348
BfmI	CTRYAG	1 cut(s)	1356
BfoI	RGCGCY	1 cut(s)	123
BfuAI	ACCTGC	2 cut(s)	2345, 2430
BglII	AGATCT	2 cut(s)	1280, 2160
BisI	GCNGC	7 cut(s)	811, 1254, 1646, 1869, 2119, 2352, 2367
BlpI	GCTNAGC	2 cut(s)	841, 1641
BlsI	GCNGC	7 cut(s)	812, 1255, 1647, 1870, 2120, 2353, 2368
Bme1390I	CCNGG	6 cut(s)	180, 561, 584, 823, 1906, 2107
Bme18I	GGWCC	1 cut(s)	253
BmeRI	GACNNNNNGTC	1 cut(s)	1524
BmeT110I	CYCGRG	1 cut(s)	2140
BmgT120I	GGNCC	3 cut(s)	253, 860, 1858
BmiI	GGNNCC	4 cut(s)	121, 289, 1468, 1903
BmrFI	CCNGG	6 cut(s)	180, 561, 584, 823, 1906, 2107
BmrI	ACTGGG	1 cut(s)	1134
BmsI	GCATC	5 cut(s)	268, 854, 1014, 1025, 1693
BmuI	ACTGGG	1 cut(s)	1134
BoxI	GACNNNNGTC	1 cut(s)	2343
BpiI	GAAGAC	1 cut(s)	1523
BpmI	CTGGAG	2 cut(s)	294, 1153
Bpu1102I	GCTNAGC	2 cut(s)	841, 1641
BpuEI	CTTGAG	1 cut(s)	1380
BpuMI	CCSGG	1 cut(s)	180
BsaHI	GRCGYC	1 cut(s)	120
BsaJI	CCNNGG	4 cut(s)	560, 588, 1337, 1435
BsaXI	ACNNNNNCTCC	6 cut(s)	1126, 1156, 1270, 1300, 1714, 1744
Bsc4I	CCNNNNNNNGG	3 cut(s)	28, 473, 589
Bse1I	ACTGG	5 cut(s)	478, 906, 1136, 1140, 2005
BseBI	CCWGG	5 cut(s)	561, 584, 823, 1906, 2107
BseDI	CCNNGG	4 cut(s)	560, 588, 1337, 1435
BseGI	GGATG	4 cut(s)	439, 1077, 1431, 1757
BseLI	CCNNNNNNNGG	3 cut(s)	28, 473, 589
BseMII	CTCAG	2 cut(s)	396, 1632
BseNI	ACTGG	5 cut(s)	478, 906, 1136, 1140, 2005
BseRI	GAGGAG	1 cut(s)	646
BseXI	GCAGC	7 cut(s)	822, 1265, 1657, 1880, 2130, 2363, 2378
BseYI	CCCAGC	2 cut(s)	281, 569
Bsh1236I	CGCG	2 cut(s)	113, 2201
Bsh1285I	CGRYCG	1 cut(s)	1737
BshFI	GGCC	6 cut(s)	21, 587, 861, 1128, 1768, 1859
BshNI	GGYRCC	2 cut(s)	119, 287
BsiEI	CGRYCG	1 cut(s)	1737
BsiHKAI	GWGCWC	1 cut(s)	1111
BsiHKCI	CYCGRG	1 cut(s)	2140
BsiSI	CCGG	2 cut(s)	22, 179
BslFI	GGGAC	2 cut(s)	1122, 2159
BslI	CCNNNNNNNGG	3 cut(s)	28, 473, 589
BsmAI	GTCTC	3 cut(s)	91, 1935, 2324
BsmBI	CGTCTC	1 cut(s)	91
BsmFI	GGGAC	2 cut(s)	1122, 2159
BsmI	GAATGC	2 cut(s)	233, 571
BsnI	GGCC	6 cut(s)	21, 587, 861, 1128, 1768, 1859
BsoBI	CYCGRG	1 cut(s)	2140
Bsp1286I	GDGCHC	1 cut(s)	1111
Bsp1407I	TGTACA	1 cut(s)	1576
Bsp1720I	GCTNAGC	2 cut(s)	841, 1641
Bsp19I	CCATGG	1 cut(s)	588
Bsp68I	TCGCGA	1 cut(s)	2201
BspACI	CCGC	2 cut(s)	15, 2116
BspANI	GGCC	6 cut(s)	21, 587, 861, 1128, 1768, 1859
BspCNI	CTCAG	2 cut(s)	397, 1633
BspFNI	CGCG	2 cut(s)	113, 2201
BspHI	TCATGA	3 cut(s)	922, 1408, 1825
BspLI	GGNNCC	4 cut(s)	121, 289, 1468, 1903
BspMI	ACCTGC	2 cut(s)	2345, 2430
BspPI	GGATC	5 cut(s)	604, 689, 1183, 2007, 2381
BspT107I	GGYRCC	2 cut(s)	119, 287
BsrGI	TGTACA	1 cut(s)	1576
BsrI	ACTGG	5 cut(s)	478, 906, 1136, 1140, 2005
BssECI	CCNNGG	4 cut(s)	560, 588, 1337, 1435
BssNI	GRCGYC	1 cut(s)	120
BssSI	CACGAG	1 cut(s)	2072
BssT1I	CCWWGG	2 cut(s)	588, 1435
Bst2BI	CACGAG	1 cut(s)	2072
Bst2UI	CCWGG	5 cut(s)	561, 584, 823, 1906, 2107
Bst4CI	ACNGT	9 cut(s)	10, 442, 488, 1087, 1421, 1945, 2155, 2214, 2382
Bst6I	CTCTTC	1 cut(s)	915
BstACI	GRCGYC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	416
BstAUI	TGTACA	1 cut(s)	1576
BstC8I	GCNNGC	4 cut(s)	655, 2199, 2280, 2469
BstDEI	CTNAG	5 cut(s)	405, 841, 1156, 1210, 1641
BstDSI	CCRYGG	2 cut(s)	588, 1337
BstF5I	GGATG	4 cut(s)	439, 1077, 1431, 1757
BstFNI	CGCG	2 cut(s)	113, 2201
BstH2I	RGCGCY	1 cut(s)	123
BstHHI	GCGC	2 cut(s)	122, 2439
BstMAI	GTCTC	3 cut(s)	91, 1935, 2324
BstMCI	CGRYCG	1 cut(s)	1737
BstMWI	GCNNNNNNNGC	7 cut(s)	119, 128, 239, 287, 416, 1865, 1874
BstNI	CCWGG	5 cut(s)	561, 584, 823, 1906, 2107
BstNSI	RCATGY	1 cut(s)	378
BstPAI	GACNNNNGTC	1 cut(s)	2343
BstSCI	CCNGG	6 cut(s)	178, 559, 582, 821, 1904, 2105
BstSFI	CTRYAG	1 cut(s)	1356
BstUI	CGCG	2 cut(s)	113, 2201
BstV1I	GCAGC	7 cut(s)	822, 1265, 1657, 1880, 2130, 2363, 2378
BstV2I	GAAGAC	1 cut(s)	1523
BstX2I	RGATCY	4 cut(s)	609, 1175, 1280, 2160
BstYI	RGATCY	4 cut(s)	609, 1175, 1280, 2160
BsuRI	GGCC	6 cut(s)	21, 587, 861, 1128, 1768, 1859
BtgI	CCRYGG	2 cut(s)	588, 1337
BtsCI	GGATG	4 cut(s)	439, 1077, 1431, 1757
BtsI	GCAGTG	1 cut(s)	1825
BtuMI	TCGCGA	1 cut(s)	2201
BveI	ACCTGC	2 cut(s)	2345, 2430
Cac8I	GCNNGC	4 cut(s)	655, 2199, 2280, 2469
CaiI	CAGNNNCTG	2 cut(s)	1712, 2039
CciI	TCATGA	3 cut(s)	922, 1408, 1825
CfoI	GCGC	2 cut(s)	122, 2439
Cfr13I	GGNCC	3 cut(s)	253, 860, 1858
CseI	GACGC	2 cut(s)	408, 2215
Csp6I	GTAC	6 cut(s)	438, 866, 1203, 1577, 2360, 2378
CviQI	GTAC	6 cut(s)	438, 866, 1203, 1577, 2360, 2378
DdeI	CTNAG	5 cut(s)	405, 841, 1156, 1210, 1641
DinI	GGCGCC	1 cut(s)	121
DriI	GACNNNNNGTC	1 cut(s)	1524
EaeI	YGGCCR	4 cut(s)	19, 585, 1126, 1766
Eam1104I	CTCTTC	1 cut(s)	915
Eam1105I	GACNNNNNGTC	1 cut(s)	1524
EarI	CTCTTC	1 cut(s)	915
Eco130I	CCWWGG	2 cut(s)	588, 1435
Eco47I	GGWCC	1 cut(s)	253
Eco57I	CTGAAG	1 cut(s)	957
Eco88I	CYCGRG	1 cut(s)	2140
EcoO109I	RGGNCCY	1 cut(s)	860
EcoRI	GAATTC	1 cut(s)	1958
EcoRII	CCWGG	5 cut(s)	559, 582, 821, 1904, 2105
EcoT14I	CCWWGG	2 cut(s)	588, 1435
EcoT22I	ATGCAT	1 cut(s)	380
EgeI	GGCGCC	1 cut(s)	121
EheI	GGCGCC	1 cut(s)	121
ErhI	CCWWGG	2 cut(s)	588, 1435
Esp3I	CGTCTC	1 cut(s)	91
FalI	AAGNNNNNCTT	2 cut(s)	1821, 1853
FaqI	GGGAC	2 cut(s)	1122, 2159
FauI	CCCGC	1 cut(s)	2123
FauNDI	CATATG	2 cut(s)	776, 2093
FbaI	TGATCA	2 cut(s)	1822, 2485
FblI	GTMKAC	1 cut(s)	422
Fnu4HI	GCNGC	7 cut(s)	811, 1254, 1646, 1869, 2119, 2352, 2367
FokI	GGATG	4 cut(s)	446, 1084, 1418, 1764
Fsp4HI	GCNGC	7 cut(s)	811, 1254, 1646, 1869, 2119, 2352, 2367
FspBI	CTAG	7 cut(s)	548, 1112, 1206, 1995, 2049, 2247, 2348
GlaI	GCGC	2 cut(s)	121, 2438
GluI	GCNGC	7 cut(s)	811, 1254, 1646, 1869, 2119, 2352, 2367
GsaI	CCCAGC	2 cut(s)	285, 573
GsuI	CTGGAG	2 cut(s)	294, 1153
HaeII	RGCGCY	1 cut(s)	123
HaeIII	GGCC	6 cut(s)	21, 587, 861, 1128, 1768, 1859
HapII	CCGG	2 cut(s)	22, 179
HgaI	GACGC	2 cut(s)	408, 2215
HhaI	GCGC	2 cut(s)	122, 2439
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	2 cut(s)	120, 2437
HinP1I	GCGC	2 cut(s)	120, 2437
HincII	GTYRAC	3 cut(s)	995, 1722, 2329
HindII	GTYRAC	3 cut(s)	995, 1722, 2329
HindIII	AAGCTT	3 cut(s)	838, 1781, 1835
HpaI	GTTAAC	1 cut(s)	2329
HpaII	CCGG	2 cut(s)	22, 179
HphI	GGTGA	6 cut(s)	488, 494, 734, 954, 1298, 1417
Hpy166II	GTNNAC	8 cut(s)	6, 79, 423, 995, 1577, 1722, 2329, 2360
Hpy188I	TCNGA	7 cut(s)	400, 433, 686, 894, 976, 1558, 1734
Hpy8I	GTNNAC	8 cut(s)	6, 79, 423, 995, 1577, 1722, 2329, 2360
Hpy99I	CGWCG	3 cut(s)	14, 137, 2342
HpyAV	CCTTC	6 cut(s)	314, 358, 473, 851, 971, 1823
HpyCH4III	ACNGT	9 cut(s)	10, 442, 488, 1087, 1421, 1945, 2155, 2214, 2382
HpyCH4IV	ACGT	1 cut(s)	84
HpyF10VI	GCNNNNNNNGC	7 cut(s)	119, 128, 239, 287, 416, 1865, 1874
HpyF3I	CTNAG	5 cut(s)	405, 841, 1156, 1210, 1641
HpySE526I	ACGT	1 cut(s)	84
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	2 cut(s)	120, 2437
KasI	GGCGCC	1 cut(s)	119
Ksp22I	TGATCA	2 cut(s)	1822, 2485
KspAI	GTTAAC	1 cut(s)	2329
LmnI	GCTCC	4 cut(s)	56, 1466, 1504, 2480
Lsp1109I	GCAGC	7 cut(s)	822, 1265, 1657, 1880, 2130, 2363, 2378
LweI	GCATC	5 cut(s)	268, 854, 1014, 1025, 1693
MaeI	CTAG	7 cut(s)	548, 1112, 1206, 1995, 2049, 2247, 2348
MaeII	ACGT	1 cut(s)	84
MfeI	CAATTG	3 cut(s)	1568, 1745, 2022
MflI	RGATCY	4 cut(s)	609, 1175, 1280, 2160
MhlI	GDGCHC	1 cut(s)	1111
MlsI	TGGCCA	3 cut(s)	587, 1128, 1768
MluNI	TGGCCA	3 cut(s)	587, 1128, 1768
Mly113I	GGCGCC	1 cut(s)	120
MlyI	GAGTC	4 cut(s)	1144, 2197, 2295, 2326
MmeI	TCCRAC	1 cut(s)	2266
Mox20I	TGGCCA	3 cut(s)	587, 1128, 1768
Mph1103I	ATGCAT	1 cut(s)	380
MroXI	GAANNNNTTC	4 cut(s)	909, 1330, 1695, 2179
MscI	TGGCCA	3 cut(s)	587, 1128, 1768
MseI	TTAA	5 cut(s)	198, 1097, 2190, 2328, 2518
MslI	CAYNNNNRTG	5 cut(s)	1167, 1407, 1797, 2239, 2395
Msp20I	TGGCCA	3 cut(s)	587, 1128, 1768
MspI	CCGG	2 cut(s)	22, 179
MspR9I	CCNGG	6 cut(s)	180, 561, 584, 823, 1906, 2107
MunI	CAATTG	3 cut(s)	1568, 1745, 2022
Mva1269I	GAATGC	2 cut(s)	233, 571
MvaI	CCWGG	5 cut(s)	561, 584, 823, 1906, 2107
MvnI	CGCG	2 cut(s)	113, 2201
MwoI	GCNNNNNNNGC	7 cut(s)	119, 128, 239, 287, 416, 1865, 1874
NarI	GGCGCC	1 cut(s)	120
NciI	CCSGG	1 cut(s)	180
NcoI	CCATGG	1 cut(s)	588
NdeI	CATATG	2 cut(s)	776, 2093
NlaIV	GGNNCC	4 cut(s)	121, 289, 1468, 1903
NmuCI	GTSAC	7 cut(s)	182, 370, 482, 960, 1423, 1919, 2227
NruI	TCGCGA	1 cut(s)	2201
NsiI	ATGCAT	1 cut(s)	380
NspI	RCATGY	1 cut(s)	378
OliI	CACNNNNGTG	1 cut(s)	2239
PaeR7I	CTCGAG	1 cut(s)	2140
PagI	TCATGA	3 cut(s)	922, 1408, 1825
PaqCI	CACCTGC	1 cut(s)	2345
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	2 cut(s)	233, 571
PdmI	GAANNNNTTC	4 cut(s)	909, 1330, 1695, 2179
PfeI	GAWTC	9 cut(s)	506, 905, 1148, 1663, 1772, 2088, 2110, 2180, 2255
PflMI	CCANNNNNTGG	1 cut(s)	473
PfoI	TCCNGGA	2 cut(s)	1904, 2105
PkrI	GCNGC	7 cut(s)	812, 1255, 1647, 1870, 2120, 2353, 2368
Ple19I	CGATCG	1 cut(s)	1737
PleI	GAGTC	4 cut(s)	1143, 2197, 2295, 2325
PluTI	GGCGCC	1 cut(s)	123
PpsI	GAGTC	4 cut(s)	1143, 2197, 2295, 2325
PshAI	GACNNNNGTC	1 cut(s)	2343
Psp6I	CCWGG	5 cut(s)	559, 582, 821, 1904, 2105
PspFI	CCCAGC	2 cut(s)	281, 569
PspGI	CCWGG	5 cut(s)	559, 582, 821, 1904, 2105
PspN4I	GGNNCC	4 cut(s)	121, 289, 1468, 1903
PspPI	GGNCC	3 cut(s)	253, 860, 1858
PspXI	VCTCGAGB	1 cut(s)	2140
PstNI	CAGNNNCTG	2 cut(s)	1712, 2039
PsuI	RGATCY	4 cut(s)	609, 1175, 1280, 2160
PvuI	CGATCG	1 cut(s)	1737
RruI	TCGCGA	1 cut(s)	2201
RsaI	GTAC	6 cut(s)	439, 867, 1204, 1578, 2361, 2379
RsaNI	GTAC	6 cut(s)	438, 866, 1203, 1577, 2360, 2378
RseI	CAYNNNNRTG	5 cut(s)	1167, 1407, 1797, 2239, 2395
SaqAI	TTAA	5 cut(s)	198, 1097, 2190, 2328, 2518
SatI	GCNGC	7 cut(s)	811, 1254, 1646, 1869, 2119, 2352, 2367
Sau96I	GGNCC	3 cut(s)	253, 860, 1858
SchI	GAGTC	4 cut(s)	1144, 2197, 2295, 2326
ScrFI	CCNGG	6 cut(s)	180, 561, 584, 823, 1906, 2107
SduI	GDGCHC	1 cut(s)	1111
SfaNI	GCATC	5 cut(s)	268, 854, 1014, 1025, 1693
SfcI	CTRYAG	1 cut(s)	1356
SfoI	GGCGCC	1 cut(s)	121
Sfr274I	CTCGAG	1 cut(s)	2140
SinI	GGWCC	1 cut(s)	253
SlaI	CTCGAG	1 cut(s)	2140
SmiMI	CAYNNNNRTG	5 cut(s)	1167, 1407, 1797, 2239, 2395
SmlI	CTYRAG	2 cut(s)	1395, 2140
SmoI	CTYRAG	2 cut(s)	1395, 2140
SpeI	ACTAGT	1 cut(s)	2048
SsiI	CCGC	2 cut(s)	15, 2116
SspDI	GGCGCC	1 cut(s)	119
SspI	AATATT	1 cut(s)	495
SspMI	CTAG	7 cut(s)	548, 1112, 1206, 1995, 2049, 2247, 2348
StyD4I	CCNGG	6 cut(s)	178, 559, 582, 821, 1904, 2105
StyI	CCWWGG	2 cut(s)	588, 1435
TaaI	ACNGT	9 cut(s)	10, 442, 488, 1087, 1421, 1945, 2155, 2214, 2382
TaiI	ACGT	1 cut(s)	87
TaqI	TCGA	6 cut(s)	144, 167, 649, 1737, 2141, 2505
TaqII	GACCGA	1 cut(s)	270
TatI	WGTACW	3 cut(s)	865, 1576, 2359
TfiI	GAWTC	9 cut(s)	506, 905, 1148, 1663, 1772, 2088, 2110, 2180, 2255
Tru1I	TTAA	5 cut(s)	198, 1097, 2190, 2328, 2518
Tru9I	TTAA	5 cut(s)	198, 1097, 2190, 2328, 2518
TseFI	GTSAC	7 cut(s)	182, 370, 482, 960, 1423, 1919, 2227
TseI	GCWGC	7 cut(s)	810, 1253, 1645, 1868, 2118, 2351, 2366
Tsp45I	GTSAC	7 cut(s)	182, 370, 482, 960, 1423, 1919, 2227
TspGWI	ACGGA	3 cut(s)	948, 1326, 2313
Van91I	CCANNNNNTGG	1 cut(s)	473
VpaK11BI	GGWCC	1 cut(s)	253
XapI	RAATTY	5 cut(s)	162, 1326, 1958, 1976, 2126
XceI	RCATGY	1 cut(s)	378
XcmI	CCANNNNNNNNNTGG	2 cut(s)	595, 620
XhoI	CTCGAG	1 cut(s)	2140
XmiI	GTMKAC	1 cut(s)	422
XmnI	GAANNNNTTC	4 cut(s)	909, 1330, 1695, 2179
XspI	CTAG	7 cut(s)	548, 1112, 1206, 1995, 2049, 2247, 2348
Zsp2I	ATGCAT	1 cut(s)	380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.