MD16G1123500.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005407
Physical Location & Seq
Forward (+)
8918407 .. 8919120
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1123500.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGATCAAATCAAGGCTGAAAAGCTTCGAGCCATGAAATGCTACAAGAAGAAGCAGAACTACAACAAGAGTAATCAATTTCTTTATAGTCTTATTGTTCATGCTCTTATTGCAGTGACATGCAGTTTGCTTTGTTCATACTCTTATTCGTTTCCTTCTCTCCACACTATCAAACACTTGCTATTTATTTCTCTTCCAAATTCTTGGTCGGGGTTTATCAACCCCAAGTGCCTCTTCATTGTGGTGAATTTTATTGTCGTATTCCTTATTGGAGAATCAAGGCTCAGCAGCGGCAAGCAATCGTCTCCAGTCACCGAGATGTACAACGAGTATGTTGAGAGAAGTCGGAATCTCAGGAGACCACTCTCCACGTTTCGAGAGAAGAAAGAGGAGAGGACCTTGCCGGAAATGTTGAATCCGATAAACAAGGGCAATGCGGAGAGTATTGAAGATAAAGAAGTTGATGAAGGTAAACAAGATGACAAGCATGATCATGATCATGACGAGGAAGACTTTAAAGAATGTGGGGAAAACGATGAAGAAGAAAAAGAAGAAGAAAAAATGGAAGGAAAAATGGAGGAAAAGGAAGAACGAGAAGAAGATGAGGCTTTAATGCCTGCTGAGGAACTAAACAAAAGAGTTGAAGACTTTATTGCAAGGGTTAATAAGCAAAGGTGGCTTGAAGCTAGATATTTGGTTTGTTGTAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

238

Amino Acids

27.89

Weight (kDa)

5.29

Isoelectric Point (pI)

62.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 74 - 95 4e-07 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 291, 437
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 199, 247
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 4 cut(s) 415, 449, 644, 683
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 2 cut(s) 25, 686
AluI AGCT 2 cut(s) 25, 686
Alw26I GTCTC 2 cut(s) 309, 352
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 288
ApoI RAATTY 2 cut(s) 199, 247
ArsI GACNNNNNNTTYG 4 cut(s) 109, 141, 191, 223
Asp700I GAANNNNTTC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 396
AsuHPI GGTGA 2 cut(s) 256, 304
AvaII GGWCC 1 cut(s) 396
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BbsI GAAGAC 2 cut(s) 516, 651
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 300
BclI TGATCA 2 cut(s) 490, 496
BcoDI GTCTC 2 cut(s) 309, 352
BfaI CTAG 1 cut(s) 687
BisI GCNGC 2 cut(s) 289, 292
BlpI GCTNAGC 1 cut(s) 284
BlsI GCNGC 2 cut(s) 290, 293
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BpiI GAAGAC 2 cut(s) 516, 651
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 621
Bpu1102I GCTNAGC 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 439
Bse8I GATNNNNATC 1 cut(s) 495
BseJI GATNNNNATC 1 cut(s) 495
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 3 cut(s) 298, 367, 612
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 404
BsmAI GTCTC 2 cut(s) 309, 352
BsmBI CGTCTC 1 cut(s) 309
Bso31I GGTCTC 1 cut(s) 352
Bsp1407I TGTACA 1 cut(s) 321
Bsp143I GATC 3 cut(s) 4, 490, 496
Bsp1720I GCTNAGC 1 cut(s) 284
BspACI CCGC 2 cut(s) 291, 437
BspCNI CTCAG 3 cut(s) 297, 366, 613
BspHI TCATGA 2 cut(s) 493, 499
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 1 cut(s) 308
BssMI GATC 3 cut(s) 4, 490, 496
Bst6I CTCTTC 2 cut(s) 198, 239
BstAUI TGTACA 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 296, 618
BstDEI CTNAG 3 cut(s) 284, 353, 621
BstKTI GATC 3 cut(s) 7, 493, 499
BstMAI GTCTC 2 cut(s) 309, 352
BstMBI GATC 3 cut(s) 4, 490, 496
BstMWI GCNNNNNNNGC 2 cut(s) 110, 676
BstNSI RCATGY 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 2 cut(s) 516, 651
BstXI CCANNNNNNTGG 1 cut(s) 204
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 296, 618
CciI TCATGA 2 cut(s) 493, 499
Cfr13I GGNCC 1 cut(s) 396
Csp6I GTAC 1 cut(s) 322
CviAII CATG 7 cut(s) 34, 101, 120, 488, 494, 500, 711
CviJI RGCY 7 cut(s) 17, 25, 32, 283, 608, 679, 686
CviKI_1 RGCY 7 cut(s) 17, 25, 32, 283, 608, 679, 686
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 284, 353, 621
DpnI GATC 3 cut(s) 6, 492, 498
DpnII GATC 3 cut(s) 4, 490, 496
DraI TTTAAA 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 198, 239
EarI CTCTTC 2 cut(s) 198, 239
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 7 cut(s) 37, 104, 123, 491, 497, 503, 714
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FatI CATG 7 cut(s) 33, 100, 119, 487, 493, 499, 710
FbaI TGATCA 2 cut(s) 490, 496
Fnu4HI GCNGC 2 cut(s) 289, 292
Fsp4HI GCNGC 2 cut(s) 289, 292
FspBI CTAG 1 cut(s) 687
GluI GCNGC 2 cut(s) 289, 292
GsuI CTGGAG 1 cut(s) 291
HapII CCGG 1 cut(s) 404
Hin1II CATG 7 cut(s) 37, 104, 123, 491, 497, 503, 714
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 3 cut(s) 275, 349, 415
HpaII CCGG 1 cut(s) 404
HphI GGTGA 2 cut(s) 256, 304
Hpy166II GTNNAC 1 cut(s) 473
Hpy188I TCNGA 2 cut(s) 348, 420
Hpy188III TCNNGA 4 cut(s) 355, 377, 494, 500
Hpy8I GTNNAC 1 cut(s) 473
HpyAV CCTTC 3 cut(s) 165, 461, 560
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 3 cut(s) 113, 123, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 676
HpyF3I CTNAG 3 cut(s) 284, 353, 621
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 7 cut(s) 37, 104, 123, 491, 497, 503, 714
Ksp22I TGATCA 2 cut(s) 490, 496
Kzo9I GATC 3 cut(s) 4, 490, 496
LpnPI CCDG 4 cut(s) 321, 340, 417, 630
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 1 cut(s) 687
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 2 cut(s) 115, 310
MalI GATC 3 cut(s) 6, 492, 498
MboI GATC 3 cut(s) 4, 490, 496
MluCI AATT 3 cut(s) 77, 199, 247
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 7 cut(s) 242, 382, 387, 499, 571, 598, 616
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 3 cut(s) 516, 611, 663
MslI CAYNNNNRTG 4 cut(s) 242, 317, 492, 498
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 1 cut(s) 404
MwoI GCNNNNNNNGC 2 cut(s) 110, 676
NdeII GATC 3 cut(s) 4, 490, 496
NlaIII CATG 7 cut(s) 37, 104, 123, 491, 497, 503, 714
NmuCI GTSAC 2 cut(s) 115, 310
NspI RCATGY 1 cut(s) 123
PagI TCATGA 2 cut(s) 493, 499
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 3 cut(s) 275, 349, 415
PkrI GCNGC 2 cut(s) 290, 293
PpuMI RGGWCCY 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 396
PspPI GGNCC 1 cut(s) 396
PspPPI RGGWCCY 1 cut(s) 396
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 4 cut(s) 242, 317, 492, 498
SaqAI TTAA 3 cut(s) 516, 611, 663
SatI GCNGC 2 cut(s) 289, 292
Sau3AI GATC 3 cut(s) 4, 490, 496
Sau96I GGNCC 1 cut(s) 396
SetI ASST 6 cut(s) 27, 374, 401, 472, 677, 688
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 4 cut(s) 242, 317, 492, 498
Sse9I AATT 3 cut(s) 77, 199, 247
SsiI CCGC 2 cut(s) 291, 437
SspMI CTAG 1 cut(s) 687
TaiI ACGT 1 cut(s) 374
TaqI TCGA 2 cut(s) 28, 376
TasI AATT 3 cut(s) 77, 199, 247
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 3 cut(s) 275, 349, 415
Tru1I TTAA 3 cut(s) 516, 611, 663
Tru9I TTAA 3 cut(s) 516, 611, 663
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 2 cut(s) 115, 310
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 2 cut(s) 115, 310
TspDTI ATGAA 6 cut(s) 50, 89, 126, 226, 480, 552
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 2 cut(s) 199, 247
XceI RCATGY 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.