Rroxscaffold_5G00371520

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005407
Physical Location & Seq
Reverse (-)
53095657 .. 53096424
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371520.1

Sequence Viewer

Length: 768 bp
ATGGATCCCATTAAGGCTCAAAAGGTTCGAGCCAGCAAGCGCTACAAGAAGAGTGATTCGTTTTTCCAGAGTCTCATTCTTCATTCCCTCCTTGCGCTAGCTTGCAGTTTATTTTGTTCCTACTCTAACTCGTTTCCCAATTCTTTTTACACTATCAAACACCTCTTGTTTAATTCTCTTCCCAATACTTGGTCGTGGTTCGTTAACCCTAGCTGCCTGTTCGTGGTAGTCAATTTCATTGTGGTGTTCCTTATTGGAGAATCGAGGATCAGCGGCAAGCAATCCTCATCTCCGGCCGATCAGGTTTACGATGAGTATGTTGCGAGGACTCGAAGTCTTAGGAGACGTCCTTCCACGACAGTTCAAGAGATCAAGAAAGAAGAGAAGGTCGTAGTGACAATGGATGGCCCGAAGGAGGACAATGCTAATGGGATTGAAGATCGCAAAGAAGTTGATGAAGTACCACTGAAAGAAGAGAGTGATGGTCAGGATGAAGATTTCGAAGAGTGCGAGGAAAATGTTATCGAAAATGAAAAAAATGAAGAAGAAGAGAAAGAAATGGAAGAAGAACACGAACAAGAAGAAGAGACAGAAATGGAAGAAGAACACGAAGAAGAAGAAAAAGAACAAGAAAAAGAGGAGGAAGAGGACGCTGTAGGGATACCGGCTGAGGAACTAAACAAAAGGGTTGAAGACTTCATTGCAAGGGTTAATAAGCAAAGGTTGCTTGAAGCTAGATTTTTATTAGTTTGTAGTACAAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

255

Amino Acids

29.72

Weight (kDa)

4.56

Isoelectric Point (pI)

75.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 68 - 89 1.8e-06 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 349
AccB7I CCANNNNNTGG 1 cut(s) 189
AciI CCGC 1 cut(s) 273
AclWI GGATC 2 cut(s) 12, 275
AcoI YGGCCR 1 cut(s) 294
AcyI GRCGYC 1 cut(s) 346
AfaI GTAC 2 cut(s) 462, 757
AfeI AGCGCT 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 189, 223, 415
AgsI TTSAA 4 cut(s) 365, 437, 692, 731
AhdI GACNNNNNGTC 1 cut(s) 333
AluBI AGCT 4 cut(s) 101, 213, 734, 764
AluI AGCT 4 cut(s) 101, 213, 734, 764
Alw26I GTCTC 3 cut(s) 77, 337, 581
AlwI GGATC 2 cut(s) 12, 275
Aor51HI AGCGCT 1 cut(s) 41
AoxI GGCC 2 cut(s) 294, 406
ApeKI GCWGC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 42, 97
AspS9I GGNCC 1 cut(s) 407
AsuII TTCGAA 1 cut(s) 501
AsuNHI GCTAGC 1 cut(s) 97
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 699
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 1 cut(s) 200
BccI CCATC 2 cut(s) 398, 476
BciVI GTATCC 1 cut(s) 654
BcoDI GTCTC 3 cut(s) 77, 337, 581
BfaI CTAG 3 cut(s) 98, 210, 735
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 43
BfuI GTATCC 1 cut(s) 654
BisI GCNGC 2 cut(s) 214, 274
BlsI GCNGC 2 cut(s) 215, 275
BmeRI GACNNNNNGTC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 6
BmtI GCTAGC 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 346
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 223, 415
Bse118I RCCGGY 1 cut(s) 664
Bse3DI GCAATG 1 cut(s) 699
BseGI GGATG 2 cut(s) 409, 496
BseLI CCNNNNNNNGG 3 cut(s) 189, 223, 415
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 660
BseRI GAGGAG 1 cut(s) 653
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 2 cut(s) 296, 408
BsiEI CGRYCG 1 cut(s) 297
BsiSI CCGG 2 cut(s) 293, 665
BslI CCNNNNNNNGG 3 cut(s) 189, 223, 415
BsmAI GTCTC 3 cut(s) 77, 337, 581
BsmBI CGTCTC 1 cut(s) 337
BsnI GGCC 2 cut(s) 296, 408
Bsp119I TTCGAA 1 cut(s) 501
Bsp143I GATC 5 cut(s) 4, 267, 298, 369, 439
BspACI CCGC 1 cut(s) 273
BspANI GGCC 2 cut(s) 296, 408
BspCNI CTCAG 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 101
BspPI GGATC 2 cut(s) 12, 275
BspT104I TTCGAA 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 664
BssMI GATC 5 cut(s) 4, 267, 298, 369, 439
BssNI GRCGYC 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 8 cut(s) 44, 183, 375, 468, 498, 543, 579, 639
BstACI GRCGYC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 724
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 5 cut(s) 34, 38, 99, 103, 278
BstDEI CTNAG 2 cut(s) 338, 669
BstF5I GGATG 2 cut(s) 409, 496
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 2 cut(s) 42, 97
BstKTI GATC 5 cut(s) 7, 270, 301, 372, 442
BstMAI GTCTC 3 cut(s) 77, 337, 581
BstMBI GATC 5 cut(s) 4, 267, 298, 369, 439
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 1 cut(s) 724
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 200
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 294
BsuI GTATCC 1 cut(s) 654
BsuRI GGCC 2 cut(s) 296, 408
BtsCI GGATG 2 cut(s) 409, 496
BtsIMutI CAGTG 1 cut(s) 464
Cac8I GCNNGC 5 cut(s) 34, 38, 99, 103, 278
CfoI GCGC 2 cut(s) 42, 97
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 659
Csp6I GTAC 2 cut(s) 461, 756
CviJI RGCY 9 cut(s) 17, 32, 101, 213, 296, 408, 668, 734, 764
CviKI_1 RGCY 9 cut(s) 17, 32, 101, 213, 296, 408, 668, 734, 764
CviQI GTAC 2 cut(s) 461, 756
DdeI CTNAG 2 cut(s) 338, 669
DpnI GATC 5 cut(s) 6, 269, 300, 371, 441
DpnII GATC 5 cut(s) 4, 267, 298, 369, 439
DriI GACNNNNNGTC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 294
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 8 cut(s) 44, 183, 375, 468, 498, 543, 579, 639
Eam1105I GACNNNNNGTC 1 cut(s) 333
EarI CTCTTC 8 cut(s) 44, 183, 375, 468, 498, 543, 579, 639
EclXI CGGCCG 1 cut(s) 294
Eco47III AGCGCT 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 337
FaiI YATR 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 214, 274
FokI GGATG 2 cut(s) 416, 503
Fsp4HI GCNGC 2 cut(s) 214, 274
FspBI CTAG 3 cut(s) 98, 210, 735
GlaI GCGC 2 cut(s) 41, 96
GluI GCNGC 2 cut(s) 214, 274
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 2 cut(s) 296, 408
HapII CCGG 2 cut(s) 293, 665
HgaI GACGC 1 cut(s) 659
HhaI GCGC 2 cut(s) 42, 97
Hin1I GRCGYC 1 cut(s) 346
Hin6I GCGC 2 cut(s) 40, 95
HinP1I GCGC 2 cut(s) 40, 95
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 4 cut(s) 56, 70, 260, 328
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 2 cut(s) 293, 665
Hpy166II GTNNAC 2 cut(s) 205, 307
Hpy188III TCNNGA 4 cut(s) 67, 365, 373, 488
Hpy8I GTNNAC 2 cut(s) 205, 307
HpyAV CCTTC 3 cut(s) 360, 379, 406
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 2 cut(s) 105, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 724
HpyF3I CTNAG 2 cut(s) 338, 669
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 1 cut(s) 346
HspAI GCGC 2 cut(s) 40, 95
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 4, 267, 298, 369, 439
LpnPI CCDG 7 cut(s) 46, 80, 230, 287, 306, 473, 678
Lsp1109I GCAGC 1 cut(s) 200
MaeI CTAG 3 cut(s) 98, 210, 735
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 394
MalI GATC 5 cut(s) 6, 269, 300, 371, 441
MboI GATC 5 cut(s) 4, 267, 298, 369, 439
MflI RGATCY 1 cut(s) 4
MluCI AATT 3 cut(s) 139, 172, 232
MlyI GAGTC 2 cut(s) 79, 322
MseI TTAA 4 cut(s) 12, 171, 204, 711
MslI CAYNNNNRTG 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 2 cut(s) 293, 665
MwoI GCNNNNNNNGC 1 cut(s) 724
NdeII GATC 5 cut(s) 4, 267, 298, 369, 439
NheI GCTAGC 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 394
NspV TTCGAA 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 2 cut(s) 56, 260
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 2 cut(s) 215, 275
PleI GAGTC 2 cut(s) 78, 322
PpsI GAGTC 2 cut(s) 78, 322
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 407
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 462, 757
RsaNI GTAC 2 cut(s) 461, 756
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 4 cut(s) 12, 171, 204, 711
SatI GCNGC 2 cut(s) 214, 274
Sau3AI GATC 5 cut(s) 4, 267, 298, 369, 439
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 2 cut(s) 79, 322
SfcI CTRYAG 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 242
Sse9I AATT 3 cut(s) 139, 172, 232
SsiI CCGC 1 cut(s) 273
SspMI CTAG 3 cut(s) 98, 210, 735
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 349
TaqI TCGA 5 cut(s) 28, 263, 331, 501, 525
TasI AATT 3 cut(s) 139, 172, 232
TatI WGTACW 1 cut(s) 755
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 2 cut(s) 56, 260
Tru1I TTAA 4 cut(s) 12, 171, 204, 711
Tru9I TTAA 4 cut(s) 12, 171, 204, 711
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 7 cut(s) 71, 226, 471, 507, 546, 555, 688
TspRI CASTG 1 cut(s) 471
Van91I CCANNNNNTGG 1 cut(s) 189
XspI CTAG 3 cut(s) 98, 210, 735
ZraI GACGTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.