MD16G1133700.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010558
Physical Location & Seq
Reverse (-)
10273141 .. 10274323
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1133700.v1.1.491

Sequence Viewer

Length: 540 bp
ATGATGTGGGTTCAGCCGCTTCCAGTGGTCATGGCGGTCAAGCCGCGGAGCTCTCCTCACGGCGGCAGCCGCAGAGCCGCCTCTGTAGTTGCCCAGAAATCCAATATTCCATCTGTAGATTCTGTATCGACGCAGCAACGGCGGATACTCCCAAACCCCACTTCCAAATCAACGACTCCATCCTCCCAAACGCAACCGCTGCTATTTTCTTCTTCCTCTGCTATTACTCCGTCCGTACGCACCGTCAAAGCTCTGCGGATTCGACCCACCGCCGAGTTGGGCCTCCTCTCTCTCCTCTTTGTCCTCTCCGCGGCTTTTGCGGCAGTTTTCTCCGTCGCCATACTTCGCATTCCAACGATAAACGCTATGAGAAGATTGGCAGTTGCAGTGGGAAAACTATCCAATGTGGTGTACCGGGAGGTTCCCGGAACGTTGTATTCTCTCAAACTTTCCGGTGTTGAGATCAACGACTTGGCCAAGCAGCTTGCTGCTCTCAGGGAACAGATTTCCACAACTGGGTTTGGCAAGAATTCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.12

Weight (kDa)

11.53

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 46, 311
AclI AACGTT 1 cut(s) 431
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 529
AfaI GTAC 2 cut(s) 237, 413
AfiI CCNNNNNNNGG 3 cut(s) 62, 310, 516
AjuI GAANNNNNNNTTGG 2 cut(s) 145, 177
AluBI AGCT 3 cut(s) 51, 251, 484
AluI AGCT 3 cut(s) 51, 251, 484
Alw21I GWGCWC 1 cut(s) 53
AoxI GGCC 2 cut(s) 280, 474
ApeKI GCWGC 5 cut(s) 66, 133, 199, 481, 488
ApoI RAATTY 1 cut(s) 529
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 2 cut(s) 416, 426
BalI TGGCCA 1 cut(s) 476
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 5 cut(s) 78, 145, 186, 475, 493
BccI CCATC 2 cut(s) 118, 187
BceAI ACGGC 2 cut(s) 76, 155
BciVI GTATCC 1 cut(s) 138
BcnI CCSGG 2 cut(s) 416, 426
BfmI CTRYAG 2 cut(s) 84, 114
BfuI GTATCC 1 cut(s) 138
Bme1390I CCNGG 2 cut(s) 416, 426
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 2 cut(s) 416, 426
BmrI ACTGGG 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 525
BplI GAGNNNNNCTC 2 cut(s) 40, 72
BpuMI CCSGG 2 cut(s) 416, 426
BsaJI CCNNGG 2 cut(s) 44, 309
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 310, 516
Bse1I ACTGG 2 cut(s) 23, 520
BseDI CCNNGG 2 cut(s) 44, 309
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 3 cut(s) 62, 310, 516
BseMII CTCAG 1 cut(s) 508
BseNI ACTGG 2 cut(s) 23, 520
BseRI GAGGAG 3 cut(s) 45, 275, 284
BseXI GCAGC 5 cut(s) 78, 145, 186, 475, 493
Bsh1236I CGCG 2 cut(s) 46, 311
BshFI GGCC 2 cut(s) 282, 476
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 415, 426, 453
BsiWI CGTACG 1 cut(s) 235
BslI CCNNNNNNNGG 3 cut(s) 62, 310, 516
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 2 cut(s) 282, 476
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 462
BspANI GGCC 2 cut(s) 282, 476
BspCNI CTCAG 1 cut(s) 507
BspFNI CGCG 2 cut(s) 46, 311
BspLI GGNNCC 1 cut(s) 423
BsrI ACTGG 2 cut(s) 23, 520
BssECI CCNNGG 2 cut(s) 44, 309
BssMI GATC 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 1 cut(s) 494
BstDSI CCRYGG 2 cut(s) 44, 309
BstF5I GGATG 1 cut(s) 179
BstFNI CGCG 2 cut(s) 46, 311
BstKTI GATC 1 cut(s) 465
BstMBI GATC 1 cut(s) 462
BstMWI GCNNNNNNNGC 5 cut(s) 69, 139, 199, 317, 320
BstSCI CCNGG 2 cut(s) 414, 424
BstSFI CTRYAG 2 cut(s) 84, 114
BstUI CGCG 2 cut(s) 46, 311
BstV1I GCAGC 5 cut(s) 78, 145, 186, 475, 493
BsuI GTATCC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 282, 476
BtgI CCRYGG 2 cut(s) 44, 309
BtsCI GGATG 1 cut(s) 179
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 30, 393
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 280
Cfr42I CCGCGG 2 cut(s) 47, 312
CseI GACGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 236, 412
CviAII CATG 1 cut(s) 31
CviQI GTAC 2 cut(s) 236, 412
DdeI CTNAG 1 cut(s) 494
DpnI GATC 1 cut(s) 464
DpnII GATC 1 cut(s) 462
EaeI YGGCCR 1 cut(s) 474
EciI GGCGGA 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 53
FaeI CATG 1 cut(s) 34
FaiI YATR 3 cut(s) 32, 341, 368
FatI CATG 1 cut(s) 30
FokI GGATG 1 cut(s) 166
FriOI GRGCYC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 282, 476
HapII CCGG 3 cut(s) 415, 426, 453
HgaI GACGC 1 cut(s) 139
Hin1II CATG 1 cut(s) 34
HinfI GANTC 3 cut(s) 119, 175, 259
HpaII CCGG 3 cut(s) 415, 426, 453
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 1 cut(s) 535
Hpy8I GTNNAC 1 cut(s) 412
Hpy99I CGWCG 2 cut(s) 133, 338
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 139, 199, 317, 320
HpyF3I CTNAG 1 cut(s) 494
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 1 cut(s) 34
KspI CCGCGG 2 cut(s) 47, 312
Kzo9I GATC 1 cut(s) 462
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 7 cut(s) 36, 107, 428, 439, 466, 481, 501
Lsp1109I GCAGC 5 cut(s) 78, 145, 186, 475, 493
MaeII ACGT 1 cut(s) 431
MalI GATC 1 cut(s) 464
MboI GATC 1 cut(s) 462
MboII GAAGA 3 cut(s) 201, 204, 384
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 1 cut(s) 529
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 9 cut(s) 66, 91, 193, 226, 293, 296, 305, 314, 412
Mox20I TGGCCA 1 cut(s) 476
MscI TGGCCA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 3 cut(s) 46, 199, 311
MspI CCGG 3 cut(s) 415, 426, 453
MspR9I CCNGG 2 cut(s) 416, 426
Mva1269I GAATGC 1 cut(s) 348
MvnI CGCG 2 cut(s) 46, 311
MwoI GCNNNNNNNGC 5 cut(s) 69, 139, 199, 317, 320
NciI CCSGG 2 cut(s) 416, 426
NdeII GATC 1 cut(s) 462
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 423
NmeAIII GCCGAG 1 cut(s) 298
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 119, 259
Pfl23II CGTACG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 424
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 431
PspLI CGTACG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 280
RsaI GTAC 2 cut(s) 237, 413
RsaNI GTAC 2 cut(s) 236, 412
SacI GAGCTC 1 cut(s) 53
SacII CCGCGG 2 cut(s) 47, 312
Sau3AI GATC 1 cut(s) 462
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 2 cut(s) 416, 426
SduI GDGCHC 1 cut(s) 53
SetI ASST 5 cut(s) 53, 253, 423, 434, 486
SfcI CTRYAG 2 cut(s) 84, 114
Sfr303I CCGCGG 2 cut(s) 47, 312
SgrBI CCGCGG 2 cut(s) 47, 312
Sse9I AATT 1 cut(s) 529
SspI AATATT 1 cut(s) 106
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 2 cut(s) 414, 424
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 128, 262
TasI AATT 1 cut(s) 529
TauI GCSGC 7 cut(s) 19, 46, 66, 72, 80, 314, 323
TfiI GAWTC 2 cut(s) 119, 259
TscAI CASTG 2 cut(s) 30, 393
TseI GCWGC 5 cut(s) 66, 133, 199, 481, 488
TspGWI ACGGA 3 cut(s) 219, 223, 322
TspRI CASTG 2 cut(s) 30, 393
XapI RAATTY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.