Rroxscaffold_5G00369600

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010558
Physical Location & Seq
Forward (+)
51406630 .. 51407624
995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369600.1

Sequence Viewer

Length: 546 bp
ATGTCGGGTCAGTTGGTTCCGGTAGCCGTGACGTTGAAGCCGGCAATCCCTTTTCACGGCGGCCGGAGAGCTTTCTTCGTCGTGTCCCGGAAACTGAATACTCCATCTTTATCGAAGTTGCAACCACCAAATATCCCATCCTCCCAAACACGACCTCTGCTTTCTTCTTCTTCTTCTTCCTCTGTAACTGCTCCGTCCGTCCAAGTCGTCCAAGCTCTGCGGATTCGACCCAGTCCCGAATTGGGCCTTGCCTCTCTCCTCTTCGTTCTCTCCACGGCTTTTGCTGCACTTTTCTCCCTTGCTATATTTTCCATTCCAACTATCAATGCTTTGAGAAAATTGGCAACTTCAGTGGAGAATCTATCCCATGTGGTCTCCGAGGAGGTACCTGGAACTCTGCTTTCTCTGAAACTCTCTGGCCTTGAGGTCAATGATTTAACCAAACAGCTTACCGGTATCAGCACACCCTTTGCTCATACTAGTTTGATTATTGGGTTTTCAAGGAAAATGGTGTCCGCAGCTCGGTGGGGGAAGAATTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.2

Weight (kDa)

11.53

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 385
AciI CCGC 3 cut(s) 60, 220, 516
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 535
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 3 cut(s) 56, 242, 522
AgeI ACCGGT 1 cut(s) 452
AgsI TTSAA 3 cut(s) 37, 501, 540
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 4 cut(s) 71, 215, 448, 521
AluI AGCT 4 cut(s) 71, 215, 448, 521
Alw26I GTCTC 1 cut(s) 379
AoxI GGCC 3 cut(s) 61, 244, 418
ApeKI GCWGC 2 cut(s) 284, 518
ApoI RAATTY 1 cut(s) 535
AsiGI ACCGGT 1 cut(s) 452
Asp718I GGTACC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 244
AsuC2I CCSGG 1 cut(s) 88
BanI GGYRCC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 271, 530
BccI CCATC 2 cut(s) 112, 145
BceAI ACGGC 3 cut(s) 11, 73, 291
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 379
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 1 cut(s) 480
BisI GCNGC 3 cut(s) 61, 285, 519
BlsI GCNGC 3 cut(s) 62, 286, 520
Bme1390I CCNGG 2 cut(s) 88, 390
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 18, 387
BmrFI CCNGG 2 cut(s) 88, 390
BmrI ACTGGG 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 273, 378
BsaWI WCCGGW 2 cut(s) 19, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 242, 522
Bse118I RCCGGY 2 cut(s) 40, 452
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 273, 378
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 56, 242, 522
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 2 cut(s) 248, 395
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 271, 530
BsgI GTGCAG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 3 cut(s) 63, 246, 420
BshNI GGYRCC 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 5 cut(s) 20, 41, 64, 88, 453
BslFI GGGAC 2 cut(s) 70, 219
BslI CCNNNNNNNGG 3 cut(s) 56, 242, 522
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 2 cut(s) 70, 219
BsnI GGCC 3 cut(s) 63, 246, 420
Bso31I GGTCTC 1 cut(s) 379
BspACI CCGC 3 cut(s) 60, 220, 516
BspANI GGCC 3 cut(s) 63, 246, 420
BspLI GGNNCC 2 cut(s) 18, 387
BspT107I GGYRCC 1 cut(s) 385
BspTNI GGTCTC 1 cut(s) 379
BsrFI RCCGGY 2 cut(s) 40, 452
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 2 cut(s) 40, 452
BssECI CCNNGG 2 cut(s) 273, 378
Bst2UI CCWGG 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 86, 388
BstV1I GCAGC 2 cut(s) 271, 530
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 3 cut(s) 63, 246, 420
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 2 cut(s) 40, 452
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 386
CspAI ACCGGT 1 cut(s) 452
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 333, 368
CviAII CATG 1 cut(s) 368
CviQI GTAC 1 cut(s) 386
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EclXI CGGCCG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 379
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 388
FaeI CATG 1 cut(s) 371
FaiI YATR 3 cut(s) 305, 369, 477
FaqI GGGAC 2 cut(s) 70, 219
FatI CATG 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 61, 285, 519
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 61, 285, 519
FspBI CTAG 1 cut(s) 480
GluI GCNGC 3 cut(s) 61, 285, 519
HaeIII GGCC 3 cut(s) 63, 246, 420
HapII CCGG 5 cut(s) 20, 41, 64, 88, 453
Hin1II CATG 1 cut(s) 371
HinfI GANTC 2 cut(s) 223, 358
HpaII CCGG 5 cut(s) 20, 41, 64, 88, 453
Hpy188I TCNGA 2 cut(s) 379, 408
Hpy188III TCNNGA 1 cut(s) 236
Hpy99I CGWCG 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 2 cut(s) 121, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 1 cut(s) 371
KpnI GGTACC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 9 cut(s) 33, 54, 77, 101, 244, 375, 402, 402, 466
Lsp1109I GCAGC 2 cut(s) 271, 530
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 28, 184
MboII GAAGA 8 cut(s) 67, 156, 159, 162, 165, 168, 253, 544
MluCI AATT 3 cut(s) 239, 338, 535
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 8 cut(s) 151, 165, 190, 262, 269, 373, 376, 418
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 1 cut(s) 437
MspI CCGG 5 cut(s) 20, 41, 64, 88, 453
MspR9I CCNGG 2 cut(s) 88, 390
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 284
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 18, 387
NmuCI GTSAC 1 cut(s) 28
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 223, 358
PflFI GACNNNGTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 86
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 3 cut(s) 62, 286, 520
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 18, 387
PspPI GGNCC 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 231
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 3 cut(s) 61, 285, 519
Sau96I GGNCC 1 cut(s) 244
ScrFI CCNGG 2 cut(s) 88, 390
SetI ASST 9 cut(s) 35, 73, 157, 217, 387, 391, 429, 450, 523
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 3 cut(s) 239, 338, 535
SsiI CCGC 3 cut(s) 60, 220, 516
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 86, 388
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 113, 226
TasI AATT 3 cut(s) 239, 338, 535
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 223, 358
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 2 cut(s) 284, 518
Tsp45I GTSAC 1 cut(s) 28
TspGWI ACGGA 2 cut(s) 183, 187
TspRI CASTG 1 cut(s) 357
Tth111I GACNNNGTC 1 cut(s) 231
XapI RAATTY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.