RchiOBHm_Chr4g0427931

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010558
Physical Location & Seq
Forward (+)
53060501 .. 53062247
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39684

Sequence Viewer

Length: 501 bp
ATGTCGGGTCAGTTGGTTCCGGTAGCCGTGACGTTGAAGCCGGCAATCCCTTTTCACGGCGGCCGGAGAGCTTTCTCCGTCGTGTCCCGGAATCTGAATACTCCATCTTCATCGAAGTTGCAACTACCAAATATCCCATCCTCCCAAACACGACCTCTGCTTTCTTATTCTTCTTCCTCTGTAACTGCTCCGTCCGTCCAAGTCGTCAAAGCTCTGCGGATTCGACCCAGTCCCGAATTGGGCCTTGCCTCACTCCTCTTCGTCCTCACCACGGCTTTTGCTGCACTTTTCTCCCTTGCTATATTTTCCATTCCAACTATCAATGCTTTGAGAAAATTGGCAACTTCAGTGGACAAGCTATCCCATGTGGTCTCCGAGGAGGTACCTGGAACTCTGCTTTCTCTGAAACTCTCTGGCCTTGAGGTCAATGATTTAGCCAAACAGCTTACCGGTATAAGGAAAATGGTGTCCGCAGCTCGGTGGGGGAAGAATTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

17.63

Weight (kDa)

11.31

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 382
AccB1I GGYRCC 1 cut(s) 382
AciI CCGC 3 cut(s) 60, 217, 471
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 384
AfiI CCNNNNNNNGG 5 cut(s) 56, 239, 271, 456, 477
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 2 cut(s) 37, 495
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 5 cut(s) 71, 212, 358, 445, 476
AluI AGCT 5 cut(s) 71, 212, 358, 445, 476
Alw26I GTCTC 1 cut(s) 376
AoxI GGCC 3 cut(s) 61, 241, 415
ApeKI GCWGC 2 cut(s) 281, 473
ApoI RAATTY 1 cut(s) 490
AsiGI ACCGGT 1 cut(s) 449
Asp718I GGTACC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 241
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 259
BanI GGYRCC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 268, 485
BccI CCATC 2 cut(s) 112, 145
BceAI ACGGC 3 cut(s) 11, 73, 288
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 376
BisI GCNGC 3 cut(s) 61, 282, 474
BlsI GCNGC 3 cut(s) 62, 283, 475
Bme1390I CCNGG 2 cut(s) 88, 387
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 2 cut(s) 18, 384
BmrFI CCNGG 2 cut(s) 88, 387
BmrI ACTGGG 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 270, 375
BsaWI WCCGGW 2 cut(s) 19, 449
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 239, 271, 456, 477
Bse118I RCCGGY 2 cut(s) 40, 449
Bse1I ACTGG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 270, 375
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 5 cut(s) 56, 239, 271, 456, 477
BseNI ACTGG 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 245, 392
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 268, 485
BsgI GTGCAG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 3 cut(s) 63, 243, 417
BshNI GGYRCC 1 cut(s) 382
BshTI ACCGGT 1 cut(s) 449
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 5 cut(s) 20, 41, 64, 88, 450
BslFI GGGAC 2 cut(s) 70, 216
BslI CCNNNNNNNGG 5 cut(s) 56, 239, 271, 456, 477
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 70, 216
BsnI GGCC 3 cut(s) 63, 243, 417
Bso31I GGTCTC 1 cut(s) 376
BspACI CCGC 3 cut(s) 60, 217, 471
BspANI GGCC 3 cut(s) 63, 243, 417
BspLI GGNNCC 2 cut(s) 18, 384
BspT107I GGYRCC 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 376
BsrFI RCCGGY 2 cut(s) 40, 449
BsrI ACTGG 1 cut(s) 228
BssAI RCCGGY 2 cut(s) 40, 449
BssECI CCNNGG 2 cut(s) 270, 375
Bst2UI CCWGG 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 86, 385
BstV1I GCAGC 2 cut(s) 268, 485
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 3 cut(s) 63, 243, 417
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 2 cut(s) 40, 449
Cfr13I GGNCC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 383
CspAI ACCGGT 1 cut(s) 449
CspCI CAANNNNNGTGG 4 cut(s) 259, 294, 330, 365
CviAII CATG 1 cut(s) 365
CviQI GTAC 1 cut(s) 383
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 376
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 385
FaeI CATG 1 cut(s) 368
FaiI YATR 3 cut(s) 302, 366, 455
FaqI GGGAC 2 cut(s) 70, 216
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 61, 282, 474
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 61, 282, 474
GluI GCNGC 3 cut(s) 61, 282, 474
HaeIII GGCC 3 cut(s) 63, 243, 417
HapII CCGG 5 cut(s) 20, 41, 64, 88, 450
Hin1II CATG 1 cut(s) 368
HinfI GANTC 2 cut(s) 91, 220
HpaII CCGG 5 cut(s) 20, 41, 64, 88, 450
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 96, 376, 405
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 2 cut(s) 121, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 1 cut(s) 368
KpnI GGTACC 1 cut(s) 386
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 9 cut(s) 33, 54, 77, 101, 241, 372, 399, 399, 463
Lsp1109I GCAGC 2 cut(s) 268, 485
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 28, 181
MboII GAAGA 5 cut(s) 99, 162, 165, 250, 499
MluCI AATT 3 cut(s) 236, 335, 490
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 9 cut(s) 151, 165, 187, 259, 266, 275, 370, 373, 415
MroNI GCCGGC 1 cut(s) 40
MspI CCGG 5 cut(s) 20, 41, 64, 88, 450
MspR9I CCNGG 2 cut(s) 88, 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 281
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 2 cut(s) 18, 384
NmuCI GTSAC 1 cut(s) 28
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 91, 220
PflFI GACNNNGTC 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 86
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 3 cut(s) 62, 283, 475
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 18, 384
PspPI GGNCC 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 228
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
SatI GCNGC 3 cut(s) 61, 282, 474
Sau96I GGNCC 1 cut(s) 241
ScrFI CCNGG 2 cut(s) 88, 387
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 3 cut(s) 236, 335, 490
SsiI CCGC 3 cut(s) 60, 217, 471
StyD4I CCNGG 2 cut(s) 86, 385
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 113, 223
TasI AATT 3 cut(s) 236, 335, 490
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 91, 220
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 2 cut(s) 281, 473
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 3 cut(s) 67, 180, 184
TspRI CASTG 1 cut(s) 354
Tth111I GACNNNGTC 1 cut(s) 228
XapI RAATTY 1 cut(s) 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.