Rmu_sc0000066.1_g000012

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000066.1
OG0010558
Physical Location & Seq
Reverse (-)
43845 .. 44644
800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000066.1_g000012.1.cds

Sequence Viewer

Length: 504 bp
atgtcgggtcagttggttccggtagccgtgacgttgaagccggcaatcccttttcacggcggccggagagctttctccgtggtgtcccggaatctgaatactccatcttcatcgaagttgcaactaccaaatatcccatcctcccaaacacgacctctgctttcttcttcttcttcttcctctgtaacagctccgtccgtccaagtcgtcaaagctctgcggattcgacccagtcccgaattgggccttgcctctctcctcttcgtcctctccacggcttttgctgcacttttctcccttgctatattttccattccaactatcaatgctttgagaaaattggcaacttcagtggacaagctatcccatgtggtctccgaggaggtacctggaactctgctttctctgaaactctctggccttgaggtcaatgatttaaccaaacagcttaccggtatcaggaaaatggtgtccgcagctcggtgggggaagaattcaaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

17.66

Weight (kDa)

11.53

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 385
AciI CCGC 3 cut(s) 60, 220, 474
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 5 cut(s) 56, 242, 274, 459, 480
AgeI ACCGGT 1 cut(s) 452
AgsI TTSAA 2 cut(s) 37, 498
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 6 cut(s) 71, 191, 215, 361, 448, 479
AluI AGCT 6 cut(s) 71, 191, 215, 361, 448, 479
Alw26I GTCTC 1 cut(s) 379
AoxI GGCC 3 cut(s) 61, 244, 418
ApeKI GCWGC 2 cut(s) 284, 476
ApoI RAATTY 1 cut(s) 493
AsiGI ACCGGT 1 cut(s) 452
Asp718I GGTACC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 244
AsuC2I CCSGG 1 cut(s) 88
BanI GGYRCC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 271, 488
BccI CCATC 2 cut(s) 112, 145
BceAI ACGGC 3 cut(s) 11, 73, 291
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 379
BisI GCNGC 3 cut(s) 61, 285, 477
BlsI GCNGC 3 cut(s) 62, 286, 478
Bme1390I CCNGG 2 cut(s) 88, 390
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 18, 387
BmrFI CCNGG 2 cut(s) 88, 390
BmrI ACTGGG 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 3 cut(s) 78, 273, 378
BsaWI WCCGGW 2 cut(s) 19, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 242, 274, 459, 480
Bse118I RCCGGY 2 cut(s) 40, 452
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 78, 273, 378
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 5 cut(s) 56, 242, 274, 459, 480
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 2 cut(s) 248, 395
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 271, 488
BsgI GTGCAG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 3 cut(s) 63, 246, 420
BshNI GGYRCC 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 5 cut(s) 20, 41, 64, 88, 453
BslFI GGGAC 2 cut(s) 70, 219
BslI CCNNNNNNNGG 5 cut(s) 56, 242, 274, 459, 480
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 2 cut(s) 70, 219
BsnI GGCC 3 cut(s) 63, 246, 420
Bso31I GGTCTC 1 cut(s) 379
BspACI CCGC 3 cut(s) 60, 220, 474
BspANI GGCC 3 cut(s) 63, 246, 420
BspLI GGNNCC 2 cut(s) 18, 387
BspT107I GGYRCC 1 cut(s) 385
BspTNI GGTCTC 1 cut(s) 379
BsrFI RCCGGY 2 cut(s) 40, 452
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 2 cut(s) 40, 452
BssECI CCNNGG 3 cut(s) 78, 273, 378
Bst2UI CCWGG 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 42
BstDSI CCRYGG 2 cut(s) 78, 273
BstF5I GGATG 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 86, 388
BstV1I GCAGC 2 cut(s) 271, 488
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 3 cut(s) 63, 246, 420
BtgI CCRYGG 2 cut(s) 78, 273
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 2 cut(s) 40, 452
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 386
CspAI ACCGGT 1 cut(s) 452
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 333, 368
CviAII CATG 1 cut(s) 368
CviQI GTAC 1 cut(s) 386
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EclXI CGGCCG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 379
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 388
FaeI CATG 1 cut(s) 371
FaiI YATR 2 cut(s) 305, 369
FaqI GGGAC 2 cut(s) 70, 219
FatI CATG 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 61, 285, 477
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 61, 285, 477
GluI GCNGC 3 cut(s) 61, 285, 477
HaeIII GGCC 3 cut(s) 63, 246, 420
HapII CCGG 5 cut(s) 20, 41, 64, 88, 453
Hin1II CATG 1 cut(s) 371
HinfI GANTC 2 cut(s) 91, 223
HpaII CCGG 5 cut(s) 20, 41, 64, 88, 453
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 96, 379, 408
Hpy188III TCNNGA 2 cut(s) 236, 460
Hpy8I GTNNAC 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 2 cut(s) 121, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 1 cut(s) 371
KpnI GGTACC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 2 cut(s) 271, 488
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 28, 184
MboII GAAGA 8 cut(s) 99, 156, 159, 162, 165, 168, 253, 502
MluCI AATT 3 cut(s) 239, 338, 493
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 9 cut(s) 151, 165, 190, 262, 269, 278, 373, 376, 418
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 1 cut(s) 437
MspI CCGG 5 cut(s) 20, 41, 64, 88, 453
MspR9I CCNGG 2 cut(s) 88, 390
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 284
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 18, 387
NmuCI GTSAC 1 cut(s) 28
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 91, 223
PflFI GACNNNGTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 86
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 3 cut(s) 62, 286, 478
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 18, 387
PspPI GGNCC 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 231
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 3 cut(s) 61, 285, 477
Sau96I GGNCC 1 cut(s) 244
ScrFI CCNGG 2 cut(s) 88, 390
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 3 cut(s) 239, 338, 493
SsiI CCGC 3 cut(s) 60, 220, 474
StyD4I CCNGG 2 cut(s) 86, 388
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 113, 226
TasI AATT 3 cut(s) 239, 338, 493
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 91, 223
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 2 cut(s) 284, 476
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 3 cut(s) 67, 183, 187
TspRI CASTG 1 cut(s) 357
Tth111I GACNNNGTC 1 cut(s) 231
XapI RAATTY 1 cut(s) 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.