Rw4G024130

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010558
Physical Location & Seq
Forward (+)
49998328 .. 49999661
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024130.1

Sequence Viewer

Length: 516 bp
ATGTCGGGTCAGTTGGTTCCGGTAGCCGTGACGTTGAAGCCGGCAATCCCTTTTCACGGCGGCCGGAGAGCTTTCTCCGTGGTGTCCCGGAATCTGAATACTCCATCTTCATCGAAGTTGCAACTACCAAATATCCCATCCTCCCAAACACGACCTCTGCTTTCTTCTTCTTCTTCCTCTGTAACTGCTCCGTCCGTCCAAGTCGTCAAAGCTCTGCGGATTCGACCCAGTCCGGAATTGGGCCTTGCCTCTCTCCTCTTCGTCCTCTCCACGGCTTTTGCTGCACTTTTCTCCCTTGCTATATTTTCCATTCCAACTATCAATGCTTTGAGAAAATTGGCAACTTCAGTGGACAAGCTATCCCATGTGGTCTCCGAGGAGGTACCTGGAACTCTGCTTTCTCTGAAACTCTCTGGCTTTGAGGTCAATGATTTAACCAAACAGCTTACCGAGCTAGGCTTAATGCATAATCTCTTTGGAAAAAAAGAAAAAAGAAAAAAGAGGGGTACTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.27

Weight (kDa)

11.07

Isoelectric Point (pI)

57.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 382
AccB1I GGYRCC 1 cut(s) 382
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 2 cut(s) 60, 217
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 384, 508
AfiI CCNNNNNNNGG 3 cut(s) 56, 239, 271
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 5 cut(s) 71, 212, 358, 445, 454
AluI AGCT 5 cut(s) 71, 212, 358, 445, 454
Alw26I GTCTC 1 cut(s) 376
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 2 cut(s) 61, 241
ApeKI GCWGC 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 241
AsuC2I CCSGG 1 cut(s) 88
BanI GGYRCC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 268
BccI CCATC 2 cut(s) 112, 145
BceAI ACGGC 3 cut(s) 11, 73, 288
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 1 cut(s) 455
BisI GCNGC 2 cut(s) 61, 282
BlsI GCNGC 2 cut(s) 62, 283
Bme1390I CCNGG 2 cut(s) 88, 387
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 2 cut(s) 18, 384
BmrFI CCNGG 2 cut(s) 88, 387
BmrI ACTGGG 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 3 cut(s) 78, 270, 375
BsaWI WCCGGW 2 cut(s) 19, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 239, 271
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 78, 270, 375
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 56, 239, 271
BseNI ACTGG 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 245, 392
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 63, 243
BshNI GGYRCC 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 5 cut(s) 20, 41, 64, 88, 233
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 3 cut(s) 56, 239, 271
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 2 cut(s) 63, 243
Bso31I GGTCTC 1 cut(s) 376
Bsp13I TCCGGA 1 cut(s) 232
BspACI CCGC 2 cut(s) 60, 217
BspANI GGCC 2 cut(s) 63, 243
BspEI TCCGGA 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 18, 384
BspT107I GGYRCC 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 3 cut(s) 78, 270, 375
Bst2UI CCWGG 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 42
BstDSI CCRYGG 2 cut(s) 78, 270
BstF5I GGATG 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 281, 451
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 86, 385
BstV1I GCAGC 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 2 cut(s) 63, 243
BtgI CCRYGG 2 cut(s) 78, 270
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 383, 507
CspCI CAANNNNNGTGG 4 cut(s) 259, 294, 330, 365
CviAII CATG 1 cut(s) 365
CviQI GTAC 2 cut(s) 383, 507
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 376
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 385
EcoT22I ATGCAT 1 cut(s) 468
FaeI CATG 1 cut(s) 368
FaiI YATR 4 cut(s) 302, 366, 468, 514
FaqI GGGAC 1 cut(s) 70
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 61, 282
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 2 cut(s) 61, 282
FspBI CTAG 1 cut(s) 455
GluI GCNGC 2 cut(s) 61, 282
HaeIII GGCC 2 cut(s) 63, 243
HapII CCGG 5 cut(s) 20, 41, 64, 88, 233
Hin1II CATG 1 cut(s) 368
HinfI GANTC 2 cut(s) 91, 220
HpaII CCGG 5 cut(s) 20, 41, 64, 88, 233
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 96, 376, 405
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 121, 284, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 451
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 1 cut(s) 368
Kpn2I TCCGGA 1 cut(s) 232
KpnI GGTACC 1 cut(s) 386
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 9 cut(s) 33, 54, 77, 101, 241, 246, 372, 399, 399
Lsp1109I GCAGC 1 cut(s) 268
MaeI CTAG 1 cut(s) 455
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 28, 181
MboII GAAGA 6 cut(s) 99, 156, 159, 162, 165, 250
MluCI AATT 2 cut(s) 236, 335
MmeI TCCRAC 1 cut(s) 338
Mph1103I ATGCAT 1 cut(s) 468
MroI TCCGGA 1 cut(s) 232
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 2 cut(s) 434, 461
MspI CCGG 5 cut(s) 20, 41, 64, 88, 233
MspR9I CCNGG 2 cut(s) 88, 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 281, 451
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 2 cut(s) 18, 384
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 468
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 91, 220
PflFI GACNNNGTC 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 2 cut(s) 62, 283
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 18, 384
PspPI GGNCC 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 228
RsaI GTAC 2 cut(s) 384, 508
RsaNI GTAC 2 cut(s) 383, 507
SaqAI TTAA 2 cut(s) 434, 461
SatI GCNGC 2 cut(s) 61, 282
Sau96I GGNCC 1 cut(s) 241
ScrFI CCNGG 2 cut(s) 88, 387
Sse9I AATT 2 cut(s) 236, 335
SsiI CCGC 2 cut(s) 60, 217
SspMI CTAG 1 cut(s) 455
StyD4I CCNGG 2 cut(s) 86, 385
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 113, 223
TasI AATT 2 cut(s) 236, 335
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 91, 220
Tru1I TTAA 2 cut(s) 434, 461
Tru9I TTAA 2 cut(s) 434, 461
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 99, 501
TspGWI ACGGA 3 cut(s) 67, 180, 184
TspRI CASTG 1 cut(s) 354
Tth111I GACNNNGTC 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XspI CTAG 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.