Rmu_sc0000066.1_g000010

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000066.1
OG0010558
Physical Location & Seq
Reverse (-)
39286 .. 39932
647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000066.1_g000010.1.cds

Sequence Viewer

Length: 468 bp
atgtcgggtcagttgtttcaagtggccgcgaccttgaagccggcaatcccttttcacggcggccggagagatttctccgtcgtgtcccggaatatgtatactccatctttatcgaagttgcaactactaaatatcccatcctcccaaacacaacccctgctttcttcttcttgttcttcctctgtaacttctccgtccgtacaactcctcagagcactgctcattcgacccacacccgaattgggccttgcctctctcctcttcgtcctcttcacggcttttgctgcacttttctcccttgctatattttccattccaactatcaatgctctgagaaaattagcaacttcagtggacaagctatcccatgtggtctcccaggaggtacctggaactctgctttctctgaaactctctggcattgaggtcaatgatttaaccaaacagctcaccagtatcaggtattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.7

Weight (kDa)

10.21

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 98
AccII CGCG 1 cut(s) 29
AciI CCGC 2 cut(s) 27, 60
AcoI YGGCCR 2 cut(s) 24, 61
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 2 cut(s) 201, 387
AfiI CCNNNNNNNGG 4 cut(s) 56, 242, 274, 459
AgsI TTSAA 2 cut(s) 20, 37
AjnI CCWGG 2 cut(s) 378, 388
AluBI AGCT 2 cut(s) 361, 448
AluI AGCT 2 cut(s) 361, 448
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 379
AoxI GGCC 3 cut(s) 24, 61, 244
ApeKI GCWGC 1 cut(s) 284
Asp718I GGTACC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 244
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 442
BanI GGYRCC 1 cut(s) 385
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 271
BccI CCATC 2 cut(s) 112, 145
BceAI ACGGC 2 cut(s) 73, 291
BciT130I CCWGG 2 cut(s) 380, 390
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 379
BisI GCNGC 3 cut(s) 27, 61, 285
BlsI GCNGC 3 cut(s) 28, 62, 286
Bme1390I CCNGG 3 cut(s) 88, 380, 390
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 1 cut(s) 387
BmrFI CCNGG 3 cut(s) 88, 380, 390
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 378
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 242, 274, 459
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 380, 390
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 4 cut(s) 56, 242, 274, 459
BseMII CTCAG 2 cut(s) 223, 323
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 2 cut(s) 197, 248
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 271
BsgI GTGCAG 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 3 cut(s) 26, 63, 246
BshNI GGYRCC 1 cut(s) 385
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 3 cut(s) 41, 64, 88
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 4 cut(s) 56, 242, 274, 459
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 3 cut(s) 26, 63, 246
Bso31I GGTCTC 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 217
BspACI CCGC 2 cut(s) 27, 60
BspANI GGCC 3 cut(s) 26, 63, 246
BspCNI CTCAG 2 cut(s) 222, 324
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 385
BspTNI GGTCTC 1 cut(s) 379
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 378
BssNAI GTATAC 1 cut(s) 99
Bst1107I GTATAC 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 380, 390
Bst6I CTCTTC 2 cut(s) 266, 275
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 209, 332
BstF5I GGATG 1 cut(s) 137
BstFNI CGCG 1 cut(s) 29
BstMAI GTCTC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 2 cut(s) 380, 390
BstSCI CCNGG 3 cut(s) 86, 378, 388
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 271
BstZ17I GTATAC 1 cut(s) 99
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 3 cut(s) 26, 63, 246
BtsCI GGATG 1 cut(s) 137
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 215, 357
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 200, 386
CspCI CAANNNNNGTGG 2 cut(s) 333, 368
CviAII CATG 1 cut(s) 368
CviJI RGCY 7 cut(s) 26, 40, 63, 246, 278, 361, 448
CviKI_1 RGCY 7 cut(s) 26, 40, 63, 246, 278, 361, 448
CviQI GTAC 2 cut(s) 200, 386
DdeI CTNAG 2 cut(s) 209, 332
EaeI YGGCCR 2 cut(s) 24, 61
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 266, 275
EarI CTCTTC 2 cut(s) 266, 275
EclXI CGGCCG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 379
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 378, 388
FaeI CATG 1 cut(s) 371
FaiI YATR 4 cut(s) 95, 99, 305, 369
FaqI GGGAC 1 cut(s) 70
FatI CATG 1 cut(s) 367
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 3 cut(s) 27, 61, 285
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 27, 61, 285
GluI GCNGC 3 cut(s) 27, 61, 285
HaeIII GGCC 3 cut(s) 26, 63, 246
HapII CCGG 3 cut(s) 41, 64, 88
Hin1II CATG 1 cut(s) 371
HpaII CCGG 3 cut(s) 41, 64, 88
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 2 cut(s) 99, 355
Hpy188I TCNGA 3 cut(s) 212, 333, 408
Hpy8I GTNNAC 2 cut(s) 99, 355
Hpy99I CGWCG 1 cut(s) 83
HpyCH4V TGCA 2 cut(s) 121, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 2 cut(s) 209, 332
Hsp92II CATG 1 cut(s) 371
KpnI GGTACC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Lsp1109I GCAGC 1 cut(s) 271
MaeIII GTNAC 1 cut(s) 184
MboII GAAGA 5 cut(s) 156, 159, 168, 253, 262
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 239, 338
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 8 cut(s) 151, 190, 218, 262, 269, 278, 376, 418
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 1 cut(s) 437
MspI CCGG 3 cut(s) 41, 64, 88
MspR9I CCNGG 3 cut(s) 88, 380, 390
MvaI CCWGG 2 cut(s) 380, 390
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 284
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 1 cut(s) 387
PdiI GCCGGC 1 cut(s) 42
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 3 cut(s) 28, 62, 286
Psp6I CCWGG 2 cut(s) 378, 388
PspGI CCWGG 2 cut(s) 378, 388
PspN4I GGNNCC 1 cut(s) 387
PspPI GGNCC 1 cut(s) 244
RsaI GTAC 2 cut(s) 201, 387
RsaNI GTAC 2 cut(s) 200, 386
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 3 cut(s) 27, 61, 285
Sau96I GGNCC 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 88, 380, 390
SduI GDGCHC 1 cut(s) 217
SetI ASST 7 cut(s) 35, 363, 387, 391, 429, 450, 464
Sse9I AATT 2 cut(s) 239, 338
SsiI CCGC 2 cut(s) 27, 60
StyD4I CCNGG 3 cut(s) 86, 378, 388
TaqI TCGA 2 cut(s) 113, 226
TasI AATT 2 cut(s) 239, 338
TauI GCSGC 2 cut(s) 29, 63
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 2 cut(s) 222, 357
TseI GCWGC 1 cut(s) 284
TspGWI ACGGA 3 cut(s) 67, 183, 187
TspRI CASTG 2 cut(s) 222, 357
XcmI CCANNNNNNNNNTGG 2 cut(s) 238, 386
XmiI GTMKAC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.