Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004449

Physical Location & Seq

Genomic Coordinates Forward (+)

21588482 .. 21591026

Gene Structure 2545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1218000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 813 bp

ATGGGGAGAGCTCCTTGCTGTGAGAAGATGGGACTGAAGAAGGGGCCATGGACCTCTGAAGAAGATCAAATTCTTACATCCTTCATCCACAAATATGGCCATGGAAATTGGCGTGCCCTGCCAAAGCAAGCTGGGTTGTTGAGATGTGGAAAGAGCTGCCGACTCCGGTGGATAAACTATTTGAGGCCGGATATTAAGAGAGGAAACTTCACAAGAGAAGAAGAGGAAGCTATCATTAAGTTGCATGAAATGCTGGGTAACAGGTGGTCAGCAATTGCAGCAAGATTACCAGGACGCACCGATAACGAAATAAAAAATGTATGGCACACCCACTTGAAAAAAAGACTCAAAGATCATACTACAACAACATCATCAACAACATCAACAAGAAGTAGTAGTAACAGTACTTCCAATGTCACAAGCCAATTTGTTGATGAACCTGAAAATTTGAATTATCCACAACCATCTTCTAGTGATGTTTCCTCCTCAGTCACAAAAGTCTCAGCTGCATTGAGTACCGATCAGGACACAATCGTCGTCAAGGGCGAAAACATGGAGTTGTCGGAAACCTTCCCCGATATTGATGATGACAGCTTCTGGTCAGAGGCACTTTCAACTGATAATTCCAGCGTCCCATTACAATTTCCAAAGGCCTCAAATGATGAACCAATATCGGAGTTTCCCATCACCAAAAACGATTCGGAGGAATTTGGTTTTAGTTTTGGTCTAAACATGGATGATGGCATGGAATTTTGGTATGATCTTTTCATTAGAACTGGTCATGGGGGAACGCCAGAATTACCAGAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

271

Amino Acids

30.44

Weight (kDa)

5.25

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	3.2e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 76	1e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	67 - 112	1.5e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	70 - 118	2.2e-08	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31180
fragaria_vesca	FvH4_2g21500 FvH4_4g03610
malus_domestica	MD05G1197600.v1.1 MD10G1184100.v1.1 MD13G1213900.v1.1 MD16G1218000.v1.1
prunus_persica	Prupe.1G039200_v2.0.a1 Prupe.8G223900_v2.0.a1
pyrus_communis	pycom05g18450 pycom10g15950 pycom16g18240
rosa_chinensis	RchiOBHm_Chr4g0392981 RchiOBHm_Chr6g0288151
rosa_laevigata	RLG00000009777 RLG00000012434
rosa_multiflora	Rmu_sc0002207.1_g000004 Rmu_sc0008339.1_g000020
rosa_roxburghii	Rroxscaffold_5G00338090 Rroxscaffold_7G00180440
rosa_rugosa	Rorug04G0003200 Rorug06G0194600
rosa_samantha	Rh4AG045200 Rh4BG041100 Rh4CG048800 Rh4DG042800 Rh6AG304900 Rh6BG310900 Rh6DG304000
rosa_wichuraiana	Rw0G014460 Rw4G003550 Rw6G026390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	533
AcoI	YGGCCR	1 cut(s)	97
AcsI	RAATTY	5 cut(s)	69, 445, 707, 749, 806
AcuI	CTGAAG	2 cut(s)	56, 78
AfaI	GTAC	2 cut(s)	406, 517
AgsI	TTSAA	3 cut(s)	337, 451, 615
AjnI	CCWGG	1 cut(s)	289
AluBI	AGCT	6 cut(s)	11, 131, 156, 230, 506, 594
AluI	AGCT	6 cut(s)	11, 131, 156, 230, 506, 594
Alw21I	GWGCWC	1 cut(s)	13
Alw26I	GTCTC	1 cut(s)	505
AlwNI	CAGNNNCTG	1 cut(s)	597
AoxI	GGCC	4 cut(s)	44, 97, 185, 651
ApeKI	GCWGC	3 cut(s)	156, 278, 506
ApoI	RAATTY	5 cut(s)	69, 445, 707, 749, 806
AspS9I	GGNCC	2 cut(s)	44, 51
AsuHPI	GGTGA	1 cut(s)	679
AvaII	GGWCC	1 cut(s)	51
BaeGI	GKGCMC	1 cut(s)	118
BalI	TGGCCA	1 cut(s)	99
BanII	GRGCYC	1 cut(s)	13
BarI	GAAGNNNNNNTAC	2 cut(s)	391, 423
Bbv12I	GWGCWC	1 cut(s)	13
BbvI	GCAGC	3 cut(s)	143, 290, 493
BccI	CCATC	4 cut(s)	22, 472, 692, 734
BciT130I	CCWGG	1 cut(s)	291
BcoDI	GTCTC	1 cut(s)	505
BfaI	CTAG	1 cut(s)	471
BisI	GCNGC	3 cut(s)	157, 279, 507
BlsI	GCNGC	3 cut(s)	158, 280, 508
BmcAI	AGTACT	1 cut(s)	406
Bme1390I	CCNGG	1 cut(s)	291
Bme18I	GGWCC	1 cut(s)	51
BmgT120I	GGNCC	2 cut(s)	44, 51
BmiI	GGNNCC	1 cut(s)	45
BmrFI	CCNGG	1 cut(s)	291
BsaJI	CCNNGG	2 cut(s)	47, 100
BsaWI	WCCGGW	1 cut(s)	165
Bse1I	ACTGG	1 cut(s)	781
BseBI	CCWGG	1 cut(s)	291
BseDI	CCNNGG	2 cut(s)	47, 100
BseGI	GGATG	3 cut(s)	77, 84, 742
BseMII	CTCAG	2 cut(s)	501, 516
BseNI	ACTGG	1 cut(s)	781
BseRI	GAGGAG	1 cut(s)	475
BseSI	GKGCMC	1 cut(s)	118
BseXI	GCAGC	3 cut(s)	143, 290, 493
BseYI	CCCAGC	2 cut(s)	131, 253
BshFI	GGCC	4 cut(s)	46, 99, 187, 653
BsiHKAI	GWGCWC	1 cut(s)	13
BsiSI	CCGG	2 cut(s)	166, 188
BslFI	GGGAC	2 cut(s)	45, 617
BsmAI	GTCTC	1 cut(s)	505
BsmFI	GGGAC	2 cut(s)	45, 617
BsnI	GGCC	4 cut(s)	46, 99, 187, 653
Bsp1286I	GDGCHC	2 cut(s)	13, 118
Bsp143I	GATC	4 cut(s)	64, 352, 520, 760
Bsp19I	CCATGG	2 cut(s)	47, 100
BspANI	GGCC	4 cut(s)	46, 99, 187, 653
BspCNI	CTCAG	2 cut(s)	500, 515
BspLI	GGNNCC	1 cut(s)	45
BsrI	ACTGG	1 cut(s)	781
BssECI	CCNNGG	2 cut(s)	47, 100
BssMI	GATC	4 cut(s)	64, 352, 520, 760
BssT1I	CCWWGG	2 cut(s)	47, 100
Bst2UI	CCWGG	1 cut(s)	291
Bst4CI	ACNGT	1 cut(s)	404
Bst6I	CTCTTC	1 cut(s)	216
BstAPI	GCANNNNNTGC	1 cut(s)	250
BstC8I	GCNNGC	2 cut(s)	114, 129
BstDEI	CTNAG	2 cut(s)	487, 502
BstDSI	CCRYGG	2 cut(s)	47, 100
BstF5I	GGATG	3 cut(s)	77, 84, 742
BstKTI	GATC	4 cut(s)	67, 355, 523, 763
BstMAI	GTCTC	1 cut(s)	505
BstMBI	GATC	4 cut(s)	64, 352, 520, 760
BstMWI	GCNNNNNNNGC	3 cut(s)	118, 250, 278
BstNI	CCWGG	1 cut(s)	291
BstSCI	CCNGG	1 cut(s)	289
BstSLI	GKGCMC	1 cut(s)	118
BstV1I	GCAGC	3 cut(s)	143, 290, 493
BstXI	CCANNNNNNTGG	1 cut(s)	95
BsuRI	GGCC	4 cut(s)	46, 99, 187, 653
BtgI	CCRYGG	2 cut(s)	47, 100
BtsCI	GGATG	3 cut(s)	77, 84, 742
Cac8I	GCNNGC	2 cut(s)	114, 129
CaiI	CAGNNNCTG	1 cut(s)	597
Cfr13I	GGNCC	2 cut(s)	44, 51
CseI	GACGC	2 cut(s)	303, 619
Csp6I	GTAC	2 cut(s)	405, 516
CviAII	CATG	7 cut(s)	48, 101, 245, 553, 733, 745, 782
CviQI	GTAC	2 cut(s)	405, 516
DdeI	CTNAG	2 cut(s)	487, 502
DpnI	GATC	4 cut(s)	66, 354, 522, 762
DpnII	GATC	4 cut(s)	64, 352, 520, 760
DrdI	GACNNNNNNGTC	1 cut(s)	533
DseDI	GACNNNNNNGTC	1 cut(s)	533
EaeI	YGGCCR	1 cut(s)	97
Eam1104I	CTCTTC	1 cut(s)	216
EarI	CTCTTC	1 cut(s)	216
Ecl136II	GAGCTC	1 cut(s)	11
Eco130I	CCWWGG	2 cut(s)	47, 100
Eco147I	AGGCCT	1 cut(s)	653
Eco24I	GRGCYC	1 cut(s)	13
Eco47I	GGWCC	1 cut(s)	51
Eco53kI	GAGCTC	1 cut(s)	11
Eco57I	CTGAAG	2 cut(s)	56, 78
EcoICRI	GAGCTC	1 cut(s)	11
EcoRII	CCWGG	1 cut(s)	289
EcoT14I	CCWWGG	2 cut(s)	47, 100
EcoT38I	GRGCYC	1 cut(s)	13
ErhI	CCWWGG	2 cut(s)	47, 100
FaeI	CATG	7 cut(s)	51, 104, 248, 556, 736, 748, 785
FaqI	GGGAC	2 cut(s)	45, 617
FatI	CATG	7 cut(s)	47, 100, 244, 552, 732, 744, 781
Fnu4HI	GCNGC	3 cut(s)	157, 279, 507
FokI	GGATG	3 cut(s)	64, 71, 749
FriOI	GRGCYC	1 cut(s)	13
Fsp4HI	GCNGC	3 cut(s)	157, 279, 507
FspBI	CTAG	1 cut(s)	471
GluI	GCNGC	3 cut(s)	157, 279, 507
GsaI	CCCAGC	2 cut(s)	135, 257
HaeIII	GGCC	4 cut(s)	46, 99, 187, 653
HapII	CCGG	2 cut(s)	166, 188
HgaI	GACGC	2 cut(s)	303, 619
Hin1II	CATG	7 cut(s)	51, 104, 248, 556, 736, 748, 785
HinfI	GANTC	3 cut(s)	162, 345, 698
HpaII	CCGG	2 cut(s)	166, 188
HphI	GGTGA	1 cut(s)	679
Hpy188I	TCNGA	5 cut(s)	58, 565, 604, 676, 703
Hpy188III	TCNNGA	1 cut(s)	524
Hpy99I	CGWCG	1 cut(s)	539
HpyAV	CCTTC	3 cut(s)	34, 91, 580
HpyCH4III	ACNGT	1 cut(s)	404
HpyCH4V	TGCA	3 cut(s)	244, 278, 509
HpyF10VI	GCNNNNNNNGC	3 cut(s)	118, 250, 278
HpyF3I	CTNAG	2 cut(s)	487, 502
Hsp92II	CATG	7 cut(s)	51, 104, 248, 556, 736, 748, 785
Kzo9I	GATC	4 cut(s)	64, 352, 520, 760
LmnI	GCTCC	1 cut(s)	16
Lsp1109I	GCAGC	3 cut(s)	143, 290, 493
MaeI	CTAG	1 cut(s)	471
MaeIII	GTNAC	4 cut(s)	257, 398, 415, 490
MalI	GATC	4 cut(s)	66, 354, 522, 762
MboI	GATC	4 cut(s)	64, 352, 520, 760
MboII	GAAGA	7 cut(s)	37, 49, 71, 74, 230, 233, 459
MfeI	CAATTG	1 cut(s)	273
MhlI	GDGCHC	2 cut(s)	13, 118
MlsI	TGGCCA	1 cut(s)	99
MluNI	TGGCCA	1 cut(s)	99
MlyI	GAGTC	2 cut(s)	156, 339
MmeI	TCCRAC	1 cut(s)	543
MnlI	CCTC	9 cut(s)	64, 177, 194, 217, 493, 496, 598, 664, 697
Mox20I	TGGCCA	1 cut(s)	99
MscI	TGGCCA	1 cut(s)	99
MseI	TTAA	2 cut(s)	195, 237
MslI	CAYNNNNRTG	1 cut(s)	93
Msp20I	TGGCCA	1 cut(s)	99
MspA1I	CMGCKG	1 cut(s)	506
MspI	CCGG	2 cut(s)	166, 188
MspR9I	CCNGG	1 cut(s)	291
MunI	CAATTG	1 cut(s)	273
MvaI	CCWGG	1 cut(s)	291
MwoI	GCNNNNNNNGC	3 cut(s)	118, 250, 278
NcoI	CCATGG	2 cut(s)	47, 100
NdeII	GATC	4 cut(s)	64, 352, 520, 760
NlaIII	CATG	7 cut(s)	51, 104, 248, 556, 736, 748, 785
NlaIV	GGNNCC	1 cut(s)	45
NmuCI	GTSAC	2 cut(s)	415, 490
PceI	AGGCCT	1 cut(s)	653
PcsI	WCGNNNNNNNCGW	1 cut(s)	543
PfeI	GAWTC	1 cut(s)	698
PkrI	GCNGC	3 cut(s)	158, 280, 508
PleI	GAGTC	2 cut(s)	156, 339
PpsI	GAGTC	2 cut(s)	156, 339
Psp124BI	GAGCTC	1 cut(s)	13
Psp6I	CCWGG	1 cut(s)	289
PspFI	CCCAGC	2 cut(s)	131, 253
PspGI	CCWGG	1 cut(s)	289
PspN4I	GGNNCC	1 cut(s)	45
PspPI	GGNCC	2 cut(s)	44, 51
PstNI	CAGNNNCTG	1 cut(s)	597
PvuII	CAGCTG	1 cut(s)	506
RsaI	GTAC	2 cut(s)	406, 517
RsaNI	GTAC	2 cut(s)	405, 516
RseI	CAYNNNNRTG	1 cut(s)	93
SacI	GAGCTC	1 cut(s)	13
SaqAI	TTAA	2 cut(s)	195, 237
SatI	GCNGC	3 cut(s)	157, 279, 507
Sau3AI	GATC	4 cut(s)	64, 352, 520, 760
Sau96I	GGNCC	2 cut(s)	44, 51
ScaI	AGTACT	1 cut(s)	406
SchI	GAGTC	2 cut(s)	156, 339
ScrFI	CCNGG	1 cut(s)	291
SduI	GDGCHC	2 cut(s)	13, 118
SinI	GGWCC	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	93
SseBI	AGGCCT	1 cut(s)	653
SspMI	CTAG	1 cut(s)	471
SstI	GAGCTC	1 cut(s)	13
StuI	AGGCCT	1 cut(s)	653
StyD4I	CCNGG	1 cut(s)	289
StyI	CCWWGG	2 cut(s)	47, 100
TaaI	ACNGT	1 cut(s)	404
TatI	WGTACW	1 cut(s)	404
TfiI	GAWTC	1 cut(s)	698
Tru1I	TTAA	2 cut(s)	195, 237
Tru9I	TTAA	2 cut(s)	195, 237
TseFI	GTSAC	2 cut(s)	415, 490
TseI	GCWGC	3 cut(s)	156, 278, 506
Tsp45I	GTSAC	2 cut(s)	415, 490
TspDTI	ATGAA	5 cut(s)	73, 261, 450, 678, 757
VpaK11BI	GGWCC	1 cut(s)	51
XapI	RAATTY	5 cut(s)	69, 445, 707, 749, 806
XspI	CTAG	1 cut(s)	471
ZrmI	AGTACT	1 cut(s)	406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.