Rw0G014460
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00647
OG0004449
Physical Location & Seq
Forward (+)
30097 .. 31814
1718 bp
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UTR
Exon/CDS
Intron
Rw0G014460.1

Sequence Viewer

Length: 822 bp
ATGGTGAGAGCTCCGTGCTGCGAGAAGATGGGGTTGAAGAAAGGGCCGTGGACGCCGGAGGAGGATCACGTACTCGTCTCTTACATTCAGCAGTACGGCCATGGAAACTGGAGGGCGCTTCCCAAACTGGCAGGGTTGTTGAGGTGTGGGAAGAGTTGCAGGCTGCGGTGGACGAACTACCTGAGGCCGGATATCAAGAGAGGGAATTTTAGCAGGGAGGAAGAGGAGGCCATCATCAACTTGCATCAAATGTTGGGGAATAGATGGTCAGCAATTGCAGCAAGATTGCCTGGTCGCACAGACAATGAAATAAAAAACGTTTGGCACACCCACTTGAAAAAGAAGCTCAGCATCAAGCAGAATTCTACCACACCAGAAGCACAAGGTCAGGAATCCGAACCCGAACCCAAACCTGAAAATACTTCATGTTATGCAAAATCCGAAAGCTTCGAGTATGGGCCGGTTTCCCCACCTCAATGTACTACTTCGAGTGCTGATGACACCTCCTCATTCACCACCAGTGAGAACAATACCTGTAGTGCTAATATGGGCATGAAAGTCGACTACGAAATGAATTTACCCCAAGCGGACGAGAGCTTTTGGTCAGAAGTACTATCAGCTGATCAGAACTGTTCCAACGAGACGGTGAGTTGCGATTTTGATCAGGCAATTGGTGCAGCAGCAGATCCACAAGTGAACGCAACTGTGGAAGTTCCTGTCGATTTTGGGTTTAGTGGCTCAAATATGTACGATAACTGCATGGATTTTTGGTACAACGTTTTCACAACAGCAGGGGAGATTTCAGAATTGCTAGAGATTTGA

Protein Analysis

273

Amino Acids

30.57

Weight (kDa)

4.88

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 6.7e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.8e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 4.9e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 117 8e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 561
AciI CCGC 2 cut(s) 166, 587
AclI AACGTT 2 cut(s) 318, 777
AclWI GGATC 2 cut(s) 72, 680
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 205, 361, 574
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 6 cut(s) 72, 95, 481, 612, 749, 773
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 2 cut(s) 37, 337
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 5 cut(s) 11, 346, 447, 597, 620
AluI AGCT 5 cut(s) 11, 346, 447, 597, 620
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 2 cut(s) 82, 635
AlwI GGATC 2 cut(s) 72, 680
AoxI GGCC 5 cut(s) 44, 97, 185, 228, 458
ApeKI GCWGC 5 cut(s) 18, 163, 278, 677, 680
ApoI RAATTY 3 cut(s) 205, 361, 574
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 44, 458
AsuHPI GGTGA 3 cut(s) 16, 505, 658
AxyI CCTNAGG 1 cut(s) 182
BanII GRGCYC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 5 cut(s) 5, 150, 290, 689, 692
BccI CCATC 3 cut(s) 22, 239, 258
BceAI ACGGC 2 cut(s) 31, 112
BcgI CGANNNNNNTGC 2 cut(s) 541, 575
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 2 cut(s) 622, 661
BcoDI GTCTC 2 cut(s) 82, 635
BfaI CTAG 1 cut(s) 812
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 119
BisI GCNGC 5 cut(s) 19, 164, 279, 678, 681
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 5 cut(s) 20, 165, 280, 679, 682
BmcAI AGTACT 1 cut(s) 612
Bme1390I CCNGG 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 44, 458
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 253, 360
BpmI CTGGAG 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 47, 100
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 3 cut(s) 113, 132, 519
Bse21I CCTNAGG 1 cut(s) 182
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 47, 100
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 173, 361
BseNI ACTGG 3 cut(s) 113, 132, 519
BseRI GAGGAG 3 cut(s) 74, 239, 496
BseXI GCAGC 5 cut(s) 5, 150, 290, 689, 692
BsgI GTGCAG 1 cut(s) 696
BshFI GGCC 5 cut(s) 46, 99, 187, 230, 460
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 3 cut(s) 56, 188, 461
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 2 cut(s) 82, 635
BsmBI CGTCTC 2 cut(s) 82, 635
BsnI GGCC 5 cut(s) 46, 99, 187, 230, 460
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 4 cut(s) 64, 622, 661, 685
Bsp1720I GCTNAGC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 2 cut(s) 166, 587
BspANI GGCC 5 cut(s) 46, 99, 187, 230, 460
BspCNI CTCAG 2 cut(s) 174, 360
BspPI GGATC 2 cut(s) 72, 680
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 3 cut(s) 113, 132, 519
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 47, 100
BssMI GATC 4 cut(s) 64, 622, 661, 685
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 632, 646, 706
Bst6I CTCTTC 2 cut(s) 146, 216
BstACI GRCGYC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 182, 347
BstDSI CCRYGG 2 cut(s) 47, 100
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 4 cut(s) 67, 625, 664, 688
BstMAI GTCTC 2 cut(s) 82, 635
BstMBI GATC 4 cut(s) 64, 622, 661, 685
BstMWI GCNNNNNNNGC 3 cut(s) 52, 278, 674
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 535
BstV1I GCAGC 5 cut(s) 5, 150, 290, 689, 692
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
Bsu36I CCTNAGG 1 cut(s) 182
BsuRI GGCC 5 cut(s) 46, 99, 187, 230, 460
BtgI CCRYGG 2 cut(s) 47, 100
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 161
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 44, 458
CseI GACGC 1 cut(s) 61
Csp6I GTAC 6 cut(s) 71, 94, 480, 611, 748, 772
CviAII CATG 4 cut(s) 101, 426, 553, 760
CviQI GTAC 6 cut(s) 71, 94, 480, 611, 748, 772
DdeI CTNAG 2 cut(s) 182, 347
DpnI GATC 4 cut(s) 66, 624, 663, 687
DpnII GATC 4 cut(s) 64, 622, 661, 685
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 146, 216
EarI CTCTTC 2 cut(s) 146, 216
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 13
Eco32I GATATC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 11
Eco81I CCTNAGG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 11
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 82, 635
FaeI CATG 4 cut(s) 104, 429, 556, 763
FaiI YATR 8 cut(s) 102, 427, 432, 456, 548, 554, 746, 761
FatI CATG 4 cut(s) 100, 425, 552, 759
FbaI TGATCA 2 cut(s) 622, 661
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 19, 164, 279, 678, 681
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 5 cut(s) 19, 164, 279, 678, 681
FspBI CTAG 1 cut(s) 812
GlaI GCGC 1 cut(s) 117
GluI GCNGC 5 cut(s) 19, 164, 279, 678, 681
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 5 cut(s) 46, 99, 187, 230, 460
HapII CCGG 3 cut(s) 56, 188, 461
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 4 cut(s) 104, 429, 556, 763
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 1 cut(s) 392
HpaII CCGG 3 cut(s) 56, 188, 461
HphI GGTGA 3 cut(s) 16, 505, 658
Hpy166II GTNNAC 4 cut(s) 51, 171, 562, 697
Hpy188I TCNGA 5 cut(s) 397, 442, 607, 627, 805
Hpy188III TCNNGA 2 cut(s) 196, 389
Hpy8I GTNNAC 4 cut(s) 51, 171, 562, 697
HpyCH4III ACNGT 3 cut(s) 632, 646, 706
HpyCH4IV ACGT 3 cut(s) 69, 318, 777
HpyCH4V TGCA 6 cut(s) 159, 244, 278, 434, 677, 759
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 278, 674
HpyF3I CTNAG 2 cut(s) 182, 347
HpySE526I ACGT 3 cut(s) 69, 318, 777
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 4 cut(s) 104, 429, 556, 763
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 2 cut(s) 622, 661
Kzo9I GATC 4 cut(s) 64, 622, 661, 685
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 5 cut(s) 5, 150, 290, 689, 692
LweI GCATC 2 cut(s) 253, 360
MaeI CTAG 1 cut(s) 812
MaeII ACGT 3 cut(s) 69, 318, 777
MalI GATC 4 cut(s) 66, 624, 663, 687
MboI GATC 4 cut(s) 64, 622, 661, 685
MboII GAAGA 4 cut(s) 37, 49, 163, 233
MfeI CAATTG 2 cut(s) 273, 669
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 6 cut(s) 205, 273, 361, 574, 669, 806
MmeI TCCRAC 1 cut(s) 660
MslI CAYNNNNRTG 1 cut(s) 475
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 3 cut(s) 56, 188, 461
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 2 cut(s) 273, 669
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 52, 278, 674
NcoI CCATGG 1 cut(s) 100
NdeII GATC 4 cut(s) 64, 622, 661, 685
NlaIII CATG 4 cut(s) 104, 429, 556, 763
PfeI GAWTC 1 cut(s) 392
PkrI GCNGC 5 cut(s) 20, 165, 280, 679, 682
Ppu21I YACGTR 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 13
Psp1406I AACGTT 2 cut(s) 318, 777
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspPI GGNCC 2 cut(s) 44, 458
PsuI RGATCY 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 6 cut(s) 72, 95, 481, 612, 749, 773
RsaNI GTAC 6 cut(s) 71, 94, 480, 611, 748, 772
RseI CAYNNNNRTG 1 cut(s) 475
SacI GAGCTC 1 cut(s) 13
SalI GTCGAC 1 cut(s) 560
SatI GCNGC 5 cut(s) 19, 164, 279, 678, 681
Sau3AI GATC 4 cut(s) 64, 622, 661, 685
Sau96I GGNCC 2 cut(s) 44, 458
ScaI AGTACT 1 cut(s) 612
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 13
SfaNI GCATC 2 cut(s) 253, 360
SfcI CTRYAG 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 475
Sse9I AATT 6 cut(s) 205, 273, 361, 574, 669, 806
SsiI CCGC 2 cut(s) 166, 587
SspMI CTAG 1 cut(s) 812
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 632, 646, 706
TaiI ACGT 3 cut(s) 72, 321, 780
TaqI TCGA 4 cut(s) 450, 488, 561, 720
TasI AATT 6 cut(s) 205, 273, 361, 574, 669, 806
TatI WGTACW 2 cut(s) 479, 610
TfiI GAWTC 1 cut(s) 392
TscAI CASTG 1 cut(s) 526
TseI GCWGC 5 cut(s) 18, 163, 278, 677, 680
TspDTI ATGAA 4 cut(s) 321, 414, 569, 587
TspRI CASTG 1 cut(s) 526
XapI RAATTY 3 cut(s) 205, 361, 574
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 1 cut(s) 812
ZrmI AGTACT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.