Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008805

Physical Location & Seq

Genomic Coordinates Reverse (-)

26153328 .. 26158241

Gene Structure 4914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1241800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGCAGTGGCAACAACAACATCCAATTCCACGACCCAAAAGAATCGCTACACCTTCACTGCTCTCCTATTCATCACCCTCTTCCTCTTCGCCATTTTCGTCTTCTCCAAACGAGCTCTCGAGCCTTCTCTTTTTCTCTACAAAGATCTCCTTCCCAAAATCCCATCTTTTTCTTCCTCGCTTACCCATTCCAATCTCTCTCTCCTCAACTCCACAACCCCACTAAAGGTAGCTGTAGACCCACCTTCTCCTCCTCTTCCTTGTCCTCCTCCTCCTCCTCCTTCTGGCACCGATTCCCCCTCCCCAGAACCAGAGGAGCCACCAACCCACCAACCCATTTCGTCCAATTCAGAAACTGATGAGCCGACGGAGTCCAATGACGGAACTGAGCAAGACCCAGGTAGTGGAAATGGGAACCCATTCGGGGAAATGGGTGAAATTGGATGTGATTTGTATGCGGGGACTTGGGTGAAAGATGAGCATTATCCGCTTTACGAACCGCGTTCTTGCCCTTATGTCGATGAAGCTTTTGATTGCCAGAGCAATGGAAGGAGTGACTCTGAGTATCTCAAATGGCGGTGGAAGCCTCAGGGATGTGATCTTCCAAGGTTTAATGCAACTGACTTCTTGGTGAGACTACGGGGTAAAAAGTTGATGCTGGTAGGAGACTCCATGAATCGGAACCAGTTTGAGTCAATCCTTTGCCTCCTCCATGAGGGCTTGCATAATAAAAGCAGAATGCGTGAGATTCATGGCCACAAAATAACTAAGGGAAGAGGCTACTTTGTTTTCAAATTCGAGGACTATGACTGTACAGTAGAATTTGTGAGGTCACATTTCCTGGTGAAGGAAGGAGTCCGCATTAATGCGCAAGGGAGTTCAAATCCAACTCTTTCAATAGATCAAATTGACAAGACTGCTAAACGTTGGAAGAGGGCTGACATTCTTGTCTTTAACACTGGCCATTGGTGGACTCATGGAAAAACTGCTCGAGGGAAAAGCTACTATAAAGAGGGAGATTATCTTTATCCAAAGTTTGATGCATCTGAGGCCTATAGAAGAGCTCTGAGGACATGGGCAAACTGGATTGATCATAATATTAATCCATCAAAGCAGTTGGTCTTCTATCGTGGCTACTCTTCTGCCCATTTCAGAGGTGGAGATTGGGATTCAGGTGGGTCATGCAATGGTGAAAAAGAACCAGTCTTAAGTGGAGCCATACTTGATAACTATCCTTTGAAAATGAAGATAGTGGATGAAGTGATCAAAGATATGCGGATTCCTGTGAGGCTGCTGAATGTGACAAGGTTGACCAATTTCCGCAAAGATGGCCATCCATCGATCTTTGGCAAGAATGCAACTGCTGGCAAAAAAGTTTCCATAAGGAAGCAAGACTGCAGCCATTGGTGCGTTCCTGGGGTCCCTGATGCATGGAATGAGCTCATCTATGCCACTTTGGTTTTACAGCAGACCAACTCAAAGAGTAAAATTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

499

Amino Acids

56.23

Weight (kDa)

8.51

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	148 - 200	1e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	201 - 484	1.7e-97	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20590 AT5G20590
fragaria_vesca	FvH4_4g06600
malus_domestica	MD16G1241800.v1.1
prunus_persica	Prupe.1G068600_v2.0.a1 Prupe.1G068600_v2.0.a1
pyrus_communis	pycom16g20260
rosa_chinensis	RchiOBHm_Chr4g0398501 RchiOBHm_Chr4g0398521
rosa_laevigata	RLG00000009351 RLG00000009358
rosa_multiflora	Rmu_sc0007985.1_g000006 Rmu_ssc0000240.1_g000023
rosa_roxburghii	Rroxscaffold_5G00343470 Rroxscaffold_5G00343480
rosa_rugosa	Rorug04G0008800
rosa_samantha	Rh4AG086900 Rh4BG083500 Rh4CG094900 Rh4CG095000 Rh4DG078300
rosa_wichuraiana	Rw4G007240 Rw4G007250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	947
Acc16I	TGCGCA	1 cut(s)	870
AccB1I	GGYRCC	1 cut(s)	287
AccB7I	CCANNNNNTGG	1 cut(s)	404
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	1 cut(s)	502
AciI	CCGC	7 cut(s)	458, 488, 500, 577, 859, 1276, 1321
AclI	AACGTT	1 cut(s)	925
AcoI	YGGCCR	3 cut(s)	754, 961, 1330
AcsI	RAATTY	3 cut(s)	794, 821, 1488
AfaI	GTAC	1 cut(s)	814
AfiI	CCNNNNNNNGG	7 cut(s)	226, 284, 404, 424, 678, 715, 847
AflII	CTTAAG	1 cut(s)	1207
AgsI	TTSAA	4 cut(s)	793, 882, 897, 1240
AjnI	CCWGG	3 cut(s)	397, 840, 1414
AluBI	AGCT	6 cut(s)	116, 233, 527, 1002, 1064, 1441
AluI	AGCT	6 cut(s)	116, 233, 527, 1002, 1064, 1441
Alw21I	GWGCWC	3 cut(s)	118, 1066, 1443
Alw26I	GTCTC	2 cut(s)	628, 660
AlwNI	CAGNNNCTG	1 cut(s)	356
Ama87I	CYCGRG	2 cut(s)	119, 990
AoxI	GGCC	4 cut(s)	754, 961, 1050, 1330
ApeKI	GCWGC	2 cut(s)	1291, 1398
ApoI	RAATTY	3 cut(s)	794, 821, 1488
AseI	ATTAAT	2 cut(s)	864, 1101
Asp700I	GAANNNNTTC	1 cut(s)	419
AspLEI	GCGC	1 cut(s)	871
AspS9I	GGNCC	1 cut(s)	1420
AsuHPI	GGTGA	6 cut(s)	67, 446, 481, 643, 856, 1202
AvaI	CYCGRG	2 cut(s)	119, 990
AvaII	GGWCC	1 cut(s)	1420
AxyI	CCTNAGG	1 cut(s)	588
BalI	TGGCCA	3 cut(s)	756, 963, 1332
BanI	GGYRCC	1 cut(s)	287
BanII	GRGCYC	3 cut(s)	118, 1066, 1443
BbsI	GAAGAC	2 cut(s)	94, 1114
Bbv12I	GWGCWC	3 cut(s)	118, 1066, 1443
BbvI	GCAGC	2 cut(s)	1278, 1410
BccI	CCATC	5 cut(s)	172, 1114, 1322, 1341, 1345
BciT130I	CCWGG	3 cut(s)	399, 842, 1416
BclI	TGATCA	2 cut(s)	1090, 1263
BcoDI	GTCTC	2 cut(s)	628, 660
BfmI	CTRYAG	3 cut(s)	234, 1054, 1396
BfrI	CTTAAG	1 cut(s)	1207
BglII	AGATCT	1 cut(s)	145
BisI	GCNGC	2 cut(s)	1292, 1399
BlsI	GCNGC	2 cut(s)	1293, 1400
Bme1390I	CCNGG	3 cut(s)	399, 842, 1416
Bme18I	GGWCC	1 cut(s)	1420
BmeT110I	CYCGRG	2 cut(s)	119, 990
BmgT120I	GGNCC	1 cut(s)	1420
BmiI	GGNNCC	7 cut(s)	289, 318, 416, 683, 1216, 1421, 1422
BmrFI	CCNGG	3 cut(s)	399, 842, 1416
BmsI	GCATC	4 cut(s)	645, 1030, 1052, 1417
BpiI	GAAGAC	2 cut(s)	94, 1114
Bsa29I	ATCGAT	1 cut(s)	1340
BsaBI	GATNNNNATC	2 cut(s)	1230, 1332
BsaJI	CCNNGG	3 cut(s)	397, 605, 1415
Bsc4I	CCNNNNNNNGG	7 cut(s)	226, 284, 404, 424, 678, 715, 847
Bse1I	ACTGG	4 cut(s)	685, 964, 1088, 1202
Bse21I	CCTNAGG	1 cut(s)	588
Bse3DI	GCAATG	2 cut(s)	550, 1192
Bse8I	GATNNNNATC	2 cut(s)	1230, 1332
BseBI	CCWGG	3 cut(s)	399, 842, 1416
BseCI	ATCGAT	1 cut(s)	1340
BseDI	CCNNGG	3 cut(s)	397, 605, 1415
BseGI	GGATG	5 cut(s)	20, 449, 599, 1261, 1333
BseJI	GATNNNNATC	2 cut(s)	1230, 1332
BseLI	CCNNNNNNNGG	7 cut(s)	226, 284, 404, 424, 678, 715, 847
BseMI	GCAATG	2 cut(s)	550, 1192
BseMII	CTCAG	5 cut(s)	378, 552, 602, 1038, 1058
BseNI	ACTGG	4 cut(s)	685, 964, 1088, 1202
BseRI	GAGGAG	9 cut(s)	194, 240, 243, 258, 261, 264, 267, 329, 698
BseXI	GCAGC	2 cut(s)	1278, 1410
Bsh1236I	CGCG	1 cut(s)	502
BshFI	GGCC	4 cut(s)	756, 963, 1052, 1332
BshNI	GGYRCC	1 cut(s)	287
BshVI	ATCGAT	1 cut(s)	1340
BsiHKAI	GWGCWC	3 cut(s)	118, 1066, 1443
BsiHKCI	CYCGRG	2 cut(s)	119, 990
BslFI	GGGAC	2 cut(s)	475, 1406
BslI	CCNNNNNNNGG	7 cut(s)	226, 284, 404, 424, 678, 715, 847
BsmAI	GTCTC	2 cut(s)	628, 660
BsmFI	GGGAC	2 cut(s)	475, 1406
BsmI	GAATGC	2 cut(s)	744, 1360
BsnI	GGCC	4 cut(s)	756, 963, 1052, 1332
BsoBI	CYCGRG	2 cut(s)	119, 990
Bsp1286I	GDGCHC	3 cut(s)	118, 1066, 1443
Bsp1407I	TGTACA	1 cut(s)	812
Bsp143I	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
BspACI	CCGC	7 cut(s)	458, 488, 500, 577, 859, 1276, 1321
BspANI	GGCC	4 cut(s)	756, 963, 1052, 1332
BspCNI	CTCAG	5 cut(s)	379, 553, 601, 1039, 1059
BspDI	ATCGAT	1 cut(s)	1340
BspFNI	CGCG	1 cut(s)	502
BspLI	GGNNCC	7 cut(s)	289, 318, 416, 683, 1216, 1421, 1422
BspMAI	CTGCAG	1 cut(s)	1400
BspQI	GCTCTTC	1 cut(s)	1054
BspT107I	GGYRCC	1 cut(s)	287
BspTI	CTTAAG	1 cut(s)	1207
BsrDI	GCAATG	2 cut(s)	550, 1192
BsrGI	TGTACA	1 cut(s)	812
BsrI	ACTGG	4 cut(s)	685, 964, 1088, 1202
BssECI	CCNNGG	3 cut(s)	397, 605, 1415
BssMI	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
BssT1I	CCWWGG	1 cut(s)	605
Bst2UI	CCWGG	3 cut(s)	399, 842, 1416
Bst4CI	ACNGT	2 cut(s)	812, 817
Bst6I	CTCTTC	7 cut(s)	86, 92, 261, 769, 926, 1054, 1144
BstAFI	CTTAAG	1 cut(s)	1207
BstAUI	TGTACA	1 cut(s)	812
BstC8I	GCNNGC	2 cut(s)	722, 1366
BstDEI	CTNAG	6 cut(s)	387, 561, 588, 768, 1047, 1067
BstENI	CCTNNNNNAGG	2 cut(s)	713, 845
BstF5I	GGATG	5 cut(s)	20, 449, 599, 1261, 1333
BstFNI	CGCG	1 cut(s)	502
BstHHI	GCGC	1 cut(s)	871
BstKTI	GATC	6 cut(s)	148, 601, 904, 1093, 1266, 1344
BstMAI	GTCTC	2 cut(s)	628, 660
BstMBI	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
BstMWI	GCNNNNNNNGC	5 cut(s)	487, 583, 1049, 1329, 1407
BstNI	CCWGG	3 cut(s)	399, 842, 1416
BstSCI	CCNGG	3 cut(s)	397, 840, 1414
BstSFI	CTRYAG	3 cut(s)	234, 1054, 1396
BstUI	CGCG	1 cut(s)	502
BstV1I	GCAGC	2 cut(s)	1278, 1410
BstV2I	GAAGAC	2 cut(s)	94, 1114
BstX2I	RGATCY	1 cut(s)	145
BstXI	CCANNNNNNTGG	1 cut(s)	545
BstYI	RGATCY	1 cut(s)	145
Bsu15I	ATCGAT	1 cut(s)	1340
Bsu36I	CCTNAGG	1 cut(s)	588
BsuRI	GGCC	4 cut(s)	756, 963, 1052, 1332
BsuTUI	ATCGAT	1 cut(s)	1340
BtsCI	GGATG	5 cut(s)	20, 449, 599, 1261, 1333
BtsI	GCAGTG	2 cut(s)	12, 57
BtsIMutI	CAGTG	3 cut(s)	12, 57, 957
Cac8I	GCNNGC	2 cut(s)	722, 1366
CaiI	CAGNNNCTG	1 cut(s)	356
CfoI	GCGC	1 cut(s)	871
Cfr13I	GGNCC	1 cut(s)	1420
ClaI	ATCGAT	1 cut(s)	1340
Csp6I	GTAC	1 cut(s)	813
CviAII	CATG	7 cut(s)	673, 713, 752, 977, 1074, 1182, 1431
CviQI	GTAC	1 cut(s)	813
DdeI	CTNAG	6 cut(s)	387, 561, 588, 768, 1047, 1067
DpnI	GATC	6 cut(s)	147, 600, 903, 1092, 1265, 1343
DpnII	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
DrdI	GACNNNNNNGTC	1 cut(s)	947
DseDI	GACNNNNNNGTC	1 cut(s)	947
EaeI	YGGCCR	3 cut(s)	754, 961, 1330
Eam1104I	CTCTTC	7 cut(s)	86, 92, 261, 769, 926, 1054, 1144
EarI	CTCTTC	7 cut(s)	86, 92, 261, 769, 926, 1054, 1144
Ecl136II	GAGCTC	3 cut(s)	116, 1064, 1441
Eco130I	CCWWGG	1 cut(s)	605
Eco147I	AGGCCT	1 cut(s)	1052
Eco24I	GRGCYC	3 cut(s)	118, 1066, 1443
Eco47I	GGWCC	1 cut(s)	1420
Eco53kI	GAGCTC	3 cut(s)	116, 1064, 1441
Eco81I	CCTNAGG	1 cut(s)	588
Eco88I	CYCGRG	2 cut(s)	119, 990
EcoICRI	GAGCTC	3 cut(s)	116, 1064, 1441
EcoNI	CCTNNNNNAGG	2 cut(s)	713, 845
EcoO109I	RGGNCCY	1 cut(s)	1420
EcoRII	CCWGG	3 cut(s)	397, 840, 1414
EcoT14I	CCWWGG	1 cut(s)	605
EcoT22I	ATGCAT	2 cut(s)	1045, 1432
EcoT38I	GRGCYC	3 cut(s)	118, 1066, 1443
ErhI	CCWWGG	1 cut(s)	605
FaeI	CATG	7 cut(s)	676, 716, 755, 980, 1077, 1185, 1434
FalI	AAGNNNNNCTT	2 cut(s)	135, 167
FaqI	GGGAC	2 cut(s)	475, 1406
FatI	CATG	7 cut(s)	672, 712, 751, 976, 1073, 1181, 1430
FauI	CCCGC	1 cut(s)	451
FbaI	TGATCA	2 cut(s)	1090, 1263
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	2 cut(s)	1292, 1399
FokI	GGATG	5 cut(s)	7, 456, 606, 1268, 1320
FriOI	GRGCYC	3 cut(s)	118, 1066, 1443
Fsp4HI	GCNGC	2 cut(s)	1292, 1399
FspI	TGCGCA	1 cut(s)	870
GlaI	GCGC	1 cut(s)	870
GluI	GCNGC	2 cut(s)	1292, 1399
HaeIII	GGCC	4 cut(s)	756, 963, 1052, 1332
HhaI	GCGC	1 cut(s)	871
Hin1II	CATG	7 cut(s)	676, 716, 755, 980, 1077, 1185, 1434
Hin6I	GCGC	1 cut(s)	869
HinP1I	GCGC	1 cut(s)	869
HincII	GTYRAC	1 cut(s)	1311
HindII	GTYRAC	1 cut(s)	1311
HindIII	AAGCTT	1 cut(s)	525
HphI	GGTGA	6 cut(s)	67, 446, 481, 643, 856, 1202
Hpy166II	GTNNAC	3 cut(s)	238, 972, 1311
Hpy188I	TCNGA	6 cut(s)	352, 562, 681, 1048, 1068, 1154
Hpy188III	TCNNGA	1 cut(s)	119
Hpy8I	GTNNAC	3 cut(s)	238, 972, 1311
Hpy99I	CGWCG	1 cut(s)	370
HpyAV	CCTTC	8 cut(s)	64, 135, 161, 255, 291, 543, 841, 845
HpyCH4III	ACNGT	2 cut(s)	812, 817
HpyCH4IV	ACGT	1 cut(s)	925
HpyCH4V	TGCA	7 cut(s)	617, 724, 1043, 1185, 1358, 1398, 1430
HpyF10VI	GCNNNNNNNGC	5 cut(s)	487, 583, 1049, 1329, 1407
HpyF3I	CTNAG	6 cut(s)	387, 561, 588, 768, 1047, 1067
HpySE526I	ACGT	1 cut(s)	925
Hsp92II	CATG	7 cut(s)	676, 716, 755, 980, 1077, 1185, 1434
HspAI	GCGC	1 cut(s)	869
KflI	GGGWCCC	1 cut(s)	1420
Ksp22I	TGATCA	2 cut(s)	1090, 1263
Kzo9I	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
LguI	GCTCTTC	1 cut(s)	1054
LmnI	GCTCC	2 cut(s)	316, 1214
Lsp1109I	GCAGC	2 cut(s)	1278, 1410
LweI	GCATC	4 cut(s)	645, 1030, 1052, 1417
MaeII	ACGT	1 cut(s)	925
MaeIII	GTNAC	3 cut(s)	554, 831, 1300
MalI	GATC	6 cut(s)	147, 600, 903, 1092, 1265, 1343
MboI	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
MflI	RGATCY	1 cut(s)	145
MhlI	GDGCHC	3 cut(s)	118, 1066, 1443
MlsI	TGGCCA	3 cut(s)	756, 963, 1332
MluCI	AATT	8 cut(s)	25, 346, 438, 794, 821, 906, 1315, 1488
MluNI	TGGCCA	3 cut(s)	756, 963, 1332
MlyI	GAGTC	6 cut(s)	380, 551, 662, 701, 864, 967
MmeI	TCCRAC	2 cut(s)	908, 911
Mox20I	TGGCCA	3 cut(s)	756, 963, 1332
Mph1103I	ATGCAT	2 cut(s)	1045, 1432
MroXI	GAANNNNTTC	1 cut(s)	419
MscI	TGGCCA	3 cut(s)	756, 963, 1332
MseI	TTAA	5 cut(s)	612, 864, 954, 1101, 1208
Msp20I	TGGCCA	3 cut(s)	756, 963, 1332
MspCI	CTTAAG	1 cut(s)	1207
MspR9I	CCNGG	3 cut(s)	399, 842, 1416
Mva1269I	GAATGC	2 cut(s)	744, 1360
MvaI	CCWGG	3 cut(s)	399, 842, 1416
MvnI	CGCG	1 cut(s)	502
MwoI	GCNNNNNNNGC	5 cut(s)	487, 583, 1049, 1329, 1407
NdeII	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
NlaIII	CATG	7 cut(s)	676, 716, 755, 980, 1077, 1185, 1434
NlaIV	GGNNCC	7 cut(s)	289, 318, 416, 683, 1216, 1421, 1422
NmuCI	GTSAC	3 cut(s)	554, 831, 1300
NsbI	TGCGCA	1 cut(s)	870
NsiI	ATGCAT	2 cut(s)	1045, 1432
PaeR7I	CTCGAG	2 cut(s)	119, 990
PceI	AGGCCT	1 cut(s)	1052
PciSI	GCTCTTC	1 cut(s)	1054
PcsI	WCGNNNNNNNCGW	1 cut(s)	96
PctI	GAATGC	2 cut(s)	744, 1360
PdmI	GAANNNNTTC	1 cut(s)	419
PfeI	GAWTC	6 cut(s)	43, 293, 676, 748, 1169, 1279
PflFI	GACNNNGTC	1 cut(s)	370
PflMI	CCANNNNNTGG	1 cut(s)	404
PkrI	GCNGC	2 cut(s)	1293, 1400
PleI	GAGTC	6 cut(s)	379, 551, 662, 700, 863, 967
PpsI	GAGTC	6 cut(s)	379, 551, 662, 700, 863, 967
PpuMI	RGGWCCY	1 cut(s)	1420
PshBI	ATTAAT	2 cut(s)	864, 1101
Psp124BI	GAGCTC	3 cut(s)	118, 1066, 1443
Psp1406I	AACGTT	1 cut(s)	925
Psp5II	RGGWCCY	1 cut(s)	1420
Psp6I	CCWGG	3 cut(s)	397, 840, 1414
PspGI	CCWGG	3 cut(s)	397, 840, 1414
PspN4I	GGNNCC	7 cut(s)	289, 318, 416, 683, 1216, 1421, 1422
PspPI	GGNCC	1 cut(s)	1420
PspPPI	RGGWCCY	1 cut(s)	1420
PspXI	VCTCGAGB	1 cut(s)	990
PstI	CTGCAG	1 cut(s)	1400
PstNI	CAGNNNCTG	1 cut(s)	356
PsuI	RGATCY	1 cut(s)	145
PsyI	GACNNNGTC	1 cut(s)	370
RsaI	GTAC	1 cut(s)	814
RsaNI	GTAC	1 cut(s)	813
SacI	GAGCTC	3 cut(s)	118, 1066, 1443
SapI	GCTCTTC	1 cut(s)	1054
SaqAI	TTAA	5 cut(s)	612, 864, 954, 1101, 1208
SatI	GCNGC	2 cut(s)	1292, 1399
Sau3AI	GATC	6 cut(s)	145, 598, 901, 1090, 1263, 1341
Sau96I	GGNCC	1 cut(s)	1420
SchI	GAGTC	6 cut(s)	380, 551, 662, 701, 864, 967
ScrFI	CCNGG	3 cut(s)	399, 842, 1416
SduI	GDGCHC	3 cut(s)	118, 1066, 1443
SfaNI	GCATC	4 cut(s)	645, 1030, 1052, 1417
SfcI	CTRYAG	3 cut(s)	234, 1054, 1396
Sfr274I	CTCGAG	2 cut(s)	119, 990
SinI	GGWCC	1 cut(s)	1420
SlaI	CTCGAG	2 cut(s)	119, 990
SmlI	CTYRAG	3 cut(s)	119, 990, 1207
SmoI	CTYRAG	3 cut(s)	119, 990, 1207
Sse9I	AATT	8 cut(s)	25, 346, 438, 794, 821, 906, 1315, 1488
SseBI	AGGCCT	1 cut(s)	1052
SsiI	CCGC	7 cut(s)	458, 488, 500, 577, 859, 1276, 1321
SspI	AATATT	1 cut(s)	1099
SstI	GAGCTC	3 cut(s)	118, 1066, 1443
StuI	AGGCCT	1 cut(s)	1052
StyD4I	CCNGG	3 cut(s)	397, 840, 1414
StyI	CCWWGG	1 cut(s)	605
TaaI	ACNGT	2 cut(s)	812, 817
TaiI	ACGT	1 cut(s)	928
TaqI	TCGA	5 cut(s)	120, 519, 798, 991, 1340
TasI	AATT	8 cut(s)	25, 346, 438, 794, 821, 906, 1315, 1488
TatI	WGTACW	1 cut(s)	812
TfiI	GAWTC	6 cut(s)	43, 293, 676, 748, 1169, 1279
Tru1I	TTAA	5 cut(s)	612, 864, 954, 1101, 1208
Tru9I	TTAA	5 cut(s)	612, 864, 954, 1101, 1208
TscAI	CASTG	3 cut(s)	12, 64, 964
TseFI	GTSAC	3 cut(s)	554, 831, 1300
TseI	GCWGC	2 cut(s)	1291, 1398
Tsp45I	GTSAC	3 cut(s)	554, 831, 1300
TspDTI	ATGAA	7 cut(s)	61, 537, 689, 740, 1259, 1272, 1482
TspGWI	ACGGA	2 cut(s)	383, 396
TspRI	CASTG	3 cut(s)	12, 64, 964
Tth111I	GACNNNGTC	1 cut(s)	370
Van91I	CCANNNNNTGG	1 cut(s)	404
Vha464I	CTTAAG	1 cut(s)	1207
VpaK11BI	GGWCC	1 cut(s)	1420
VspI	ATTAAT	2 cut(s)	864, 1101
XagI	CCTNNNNNAGG	2 cut(s)	713, 845
XapI	RAATTY	3 cut(s)	794, 821, 1488
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1154
XhoI	CTCGAG	2 cut(s)	119, 990
XmiI	GTMKAC	1 cut(s)	237
XmnI	GAANNNNTTC	1 cut(s)	419
Zsp2I	ATGCAT	2 cut(s)	1045, 1432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.