Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0008805

Physical Location & Seq

Genomic Coordinates Reverse (-)

15173202 .. 15178407

Gene Structure 5206 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G007250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCAGTGGCAACACCCGGTAATTCTACCCCCCAAAAGAGCCGTTACGCCTTCATCGCTATCCTATTCATCACCCTCTTCCTCTTCACCATTTTCGTCTTCACCAAACGAGCCCTTGATCCCTCTCTCTTTCTCTACAAGGACTACTTTACCCAAATCCCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGCTGCTGCTGCTTCTCCTTCATCTTCTTCGTCTTCAACTAACAATTCCCAAATCTCTTTGTCCCAATCCACCTCTGCACCTACAGCAGACCCACCTTCTTCTTTGCTCCAATCCACCTCTGCACAACCAACCCAGGAACCCAGTTCACGAAGTGAGTTCAGCGATGAAAATGTGCAAGACCCAGATCTTGAAAATCAAAATGTGCAAGACCCAGATCGTGAAAATCAAAATGTGGAAGACCCAGACCTGAAGGAGTCCAATGACCAAAATGGTGGTGAAAATGAGAACCCAGATGGTAAAATGGGTGAAATTCCGTGTGATTTGTACGTGGGAAGTTGGGTGAGAGATGAGCAATACCCGCTTTACAAACCGGGTTCATGCCCTTATGTTGATGAAGCTTTTGATTGCCAGAGCAATGGGAGGAGAGACTCTGAGTATCTCAAATGGCGGTGGAAACCTGACGGCTGTGATCTTCCAAGGTTTAATGCAACAGATTTTTTGGTGAGGCTACGGGGTAAAAGGTTGATGCTCGTAGGAGATTCCATGAATCGGAACCAGTTTGAGTCAATATTATGCCTCCTCCGAGAAGGCTTACATAATAAGAGCAGAATGTATGAGGTCCATGGCCACAAGATATCCAAGGGAAGAGGCTATTTTGTTTTCAAATTCCAGGACTATGACTGTACGGTGGAATTTGTGAGGTCGCATTTCCTTGTGAAAGAAGGAGTCCGCTTTAATGCTCAAGGGAATTCAAATCCAACTCTATCAATGGATCAAATTGACAAGACAGCTAAACGTTGGAAGAAGGCTGACATTCTTGTCTTTAATACTGGCCACTGGTGGACTCATGGAAAGACTGCTCGTGGGAAAAACTACTACAAAGAAGGAGATTATCTCTATCCAAAATTTGATGCATCTGAGGCCTATAGACGGGCCCTGAAGACCTGGGCTAGCTGGGTTGAGCTTAATGTGAATCCAAAAAAGCAGTTGGTCTTCTATCGTGGGTACTCTTCTTCACATTTCAGAGGTGGAGATTGGGATTCAGGTGGGTCATGCAATGGTGAAAAAGAACCAGCCTTCTCTGGGGCCATACTGGATAGCTACCCTTTGAAAATGAAGATAGTGGATGAAGTAATCAAAGAAATGAAGGTTCCAGTGAAAGTATTGAATGTGACAAGGTTGACCAATTTCCGCAAAGATGGCCATCCATCAATCTACGGTAAGAAAGGTTCCACCAGGAAGCAAGACTGCAGCCATTGGTGTGTTCCTGGAGTCCCTGATGCATGGAATGAGCTAATCTATGCCACTCTGGTTTTTCAGCAGACCAAATCAAAGAGTACAGTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

516

Amino Acids

58.25

Weight (kDa)

8.2

Isoelectric Point (pI)

44.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	173 - 224	1.4e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	225 - 502	1.4e-98	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20590 AT5G20590
fragaria_vesca	FvH4_4g06600
malus_domestica	MD16G1241800.v1.1
prunus_persica	Prupe.1G068600_v2.0.a1 Prupe.1G068600_v2.0.a1
pyrus_communis	pycom16g20260
rosa_chinensis	RchiOBHm_Chr4g0398501 RchiOBHm_Chr4g0398521
rosa_laevigata	RLG00000009351 RLG00000009358
rosa_multiflora	Rmu_sc0007985.1_g000006 Rmu_ssc0000240.1_g000023
rosa_roxburghii	Rroxscaffold_5G00343470 Rroxscaffold_5G00343480
rosa_rugosa	Rorug04G0008800
rosa_samantha	Rh4AG086900 Rh4BG083500 Rh4CG094900 Rh4CG095000 Rh4DG078300
rosa_wichuraiana	Rw4G007240 Rw4G007250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1019
AciI	CCGC	4 cut(s)	560, 649, 931, 1393
AclI	AACGTT	1 cut(s)	997
AclWI	GGATC	2 cut(s)	113, 981
AcoI	YGGCCR	3 cut(s)	826, 1033, 1402
AcsI	RAATTY	5 cut(s)	510, 866, 893, 949, 1106
AcuI	CTGAAG	2 cut(s)	470, 1160
AdeI	CACNNNGTG	1 cut(s)	353
AfaI	GTAC	4 cut(s)	527, 886, 1208, 1540
AfiI	CCNNNNNNNGG	3 cut(s)	750, 1131, 1284
AgsI	TTSAA	6 cut(s)	237, 392, 865, 954, 1312, 1369
AjnI	CCWGG	5 cut(s)	333, 870, 1145, 1436, 1468
AluBI	AGCT	6 cut(s)	599, 992, 1155, 1165, 1302, 1495
AluI	AGCT	6 cut(s)	599, 992, 1155, 1165, 1302, 1495
Alw26I	GTCTC	1 cut(s)	621
AlwI	GGATC	2 cut(s)	113, 981
AoxI	GGCC	6 cut(s)	826, 1033, 1122, 1134, 1287, 1402
ApaI	GGGCCC	1 cut(s)	1138
ApeKI	GCWGC	4 cut(s)	203, 206, 209, 1452
ApoI	RAATTY	5 cut(s)	510, 866, 893, 949, 1106
AspS9I	GGNCC	4 cut(s)	820, 1134, 1135, 1287
AsuC2I	CCSGG	2 cut(s)	18, 573
AsuHPI	GGTGA	8 cut(s)	64, 79, 94, 488, 518, 553, 715, 1274
AsuNHI	GCTAGC	1 cut(s)	1151
AvaII	GGWCC	1 cut(s)	820
BaeGI	GKGCMC	1 cut(s)	1138
BalI	TGGCCA	3 cut(s)	828, 1035, 1404
BanII	GRGCYC	2 cut(s)	115, 1138
BauI	CACGAG	1 cut(s)	1062
BbsI	GAAGAC	5 cut(s)	91, 225, 444, 1148, 1186
BbvI	GCAGC	4 cut(s)	190, 193, 196, 1464
BccI	CCATC	4 cut(s)	488, 1394, 1413, 1417
BceAI	ACGGC	2 cut(s)	27, 679
BciT130I	CCWGG	5 cut(s)	335, 872, 1147, 1438, 1470
BcnI	CCSGG	2 cut(s)	18, 573
BcoDI	GTCTC	1 cut(s)	621
BfaI	CTAG	1 cut(s)	1152
BfmI	CTRYAG	3 cut(s)	282, 1126, 1450
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	4 cut(s)	204, 207, 210, 1453
BlsI	GCNGC	4 cut(s)	205, 208, 211, 1454
Bme1390I	CCNGG	7 cut(s)	18, 335, 573, 872, 1147, 1438, 1470
Bme18I	GGWCC	1 cut(s)	820
BmgT120I	GGNCC	4 cut(s)	820, 1134, 1135, 1287
BmiI	GGNNCC	6 cut(s)	339, 755, 1136, 1288, 1353, 1432
BmrFI	CCNGG	7 cut(s)	18, 335, 573, 872, 1147, 1438, 1470
BmrI	ACTGGG	1 cut(s)	336
BmsI	GCATC	4 cut(s)	717, 1102, 1124, 1471
BmtI	GCTAGC	1 cut(s)	1155
BmuI	ACTGGG	1 cut(s)	336
BpiI	GAAGAC	5 cut(s)	91, 225, 444, 1148, 1186
BplI	GAGNNNNNCTC	2 cut(s)	1080, 1112
BpmI	CTGGAG	1 cut(s)	1491
BpuEI	CTTGAG	1 cut(s)	927
BpuMI	CCSGG	2 cut(s)	18, 573
BsaAI	YACGTR	1 cut(s)	529
BsaBI	GATNNNNATC	1 cut(s)	1404
BsaJI	CCNNGG	5 cut(s)	333, 677, 823, 840, 1146
Bsc4I	CCNNNNNNNGG	3 cut(s)	750, 1131, 1284
Bse1I	ACTGG	6 cut(s)	342, 757, 1036, 1043, 1299, 1355
Bse3DI	GCAATG	2 cut(s)	622, 1264
Bse8I	GATNNNNATC	1 cut(s)	1404
BseBI	CCWGG	5 cut(s)	335, 872, 1147, 1438, 1470
BseDI	CCNNGG	5 cut(s)	333, 677, 823, 840, 1146
BseGI	GGATG	2 cut(s)	1333, 1405
BseJI	GATNNNNATC	1 cut(s)	1404
BseLI	CCNNNNNNNGG	3 cut(s)	750, 1131, 1284
BseMI	GCAATG	2 cut(s)	622, 1264
BseMII	CTCAG	2 cut(s)	624, 1110
BseNI	ACTGG	6 cut(s)	342, 757, 1036, 1043, 1299, 1355
BseRI	GAGGAG	2 cut(s)	637, 770
BseSI	GKGCMC	1 cut(s)	1138
BseXI	GCAGC	4 cut(s)	190, 193, 196, 1464
BseYI	CCCAGC	1 cut(s)	1155
BsgI	GTGCAG	2 cut(s)	261, 306
BshFI	GGCC	6 cut(s)	828, 1035, 1124, 1136, 1289, 1404
BsiSI	CCGG	2 cut(s)	18, 572
BslFI	GGGAC	2 cut(s)	247, 1460
BslI	CCNNNNNNNGG	3 cut(s)	750, 1131, 1284
BsmAI	GTCTC	1 cut(s)	621
BsmFI	GGGAC	2 cut(s)	247, 1460
BsnI	GGCC	6 cut(s)	828, 1035, 1124, 1136, 1289, 1404
Bsp120I	GGGCCC	1 cut(s)	1134
Bsp1286I	GDGCHC	2 cut(s)	115, 1138
Bsp143I	GATC	5 cut(s)	118, 385, 415, 670, 973
Bsp19I	CCATGG	1 cut(s)	823
BspACI	CCGC	4 cut(s)	560, 649, 931, 1393
BspANI	GGCC	6 cut(s)	828, 1035, 1124, 1136, 1289, 1404
BspCNI	CTCAG	2 cut(s)	625, 1111
BspLI	GGNNCC	6 cut(s)	339, 755, 1136, 1288, 1353, 1432
BspMAI	CTGCAG	1 cut(s)	1454
BspOI	GCTAGC	1 cut(s)	1155
BspPI	GGATC	2 cut(s)	113, 981
BsrDI	GCAATG	2 cut(s)	622, 1264
BsrI	ACTGG	6 cut(s)	342, 757, 1036, 1043, 1299, 1355
BssECI	CCNNGG	5 cut(s)	333, 677, 823, 840, 1146
BssMI	GATC	5 cut(s)	118, 385, 415, 670, 973
BssSI	CACGAG	1 cut(s)	1062
BssT1I	CCWWGG	3 cut(s)	677, 823, 840
Bst2BI	CACGAG	1 cut(s)	1062
Bst2UI	CCWGG	5 cut(s)	335, 872, 1147, 1438, 1470
Bst4CI	ACNGT	4 cut(s)	884, 889, 1421, 1543
Bst6I	CTCTTC	5 cut(s)	83, 89, 169, 841, 1216
BstBAI	YACGTR	1 cut(s)	529
BstC8I	GCNNGC	1 cut(s)	1153
BstDEI	CTNAG	2 cut(s)	633, 1119
BstDSI	CCRYGG	1 cut(s)	823
BstF5I	GGATG	2 cut(s)	1333, 1405
BstKTI	GATC	5 cut(s)	121, 388, 418, 673, 976
BstMAI	GTCTC	1 cut(s)	621
BstMBI	GATC	5 cut(s)	118, 385, 415, 670, 973
BstMWI	GCNNNNNNNGC	6 cut(s)	56, 209, 284, 559, 1121, 1401
BstNI	CCWGG	5 cut(s)	335, 872, 1147, 1438, 1470
BstSCI	CCNGG	7 cut(s)	16, 333, 571, 870, 1145, 1436, 1468
BstSFI	CTRYAG	3 cut(s)	282, 1126, 1450
BstSLI	GKGCMC	1 cut(s)	1138
BstV1I	GCAGC	4 cut(s)	190, 193, 196, 1464
BstV2I	GAAGAC	5 cut(s)	91, 225, 444, 1148, 1186
BstX2I	RGATCY	1 cut(s)	385
BstXI	CCANNNNNNTGG	2 cut(s)	473, 617
BstYI	RGATCY	1 cut(s)	385
BsuRI	GGCC	6 cut(s)	828, 1035, 1124, 1136, 1289, 1404
BtgI	CCRYGG	1 cut(s)	823
BtgZI	GCGATG	2 cut(s)	40, 378
BtsCI	GGATG	2 cut(s)	1333, 1405
BtsI	GCAGTG	1 cut(s)	12
BtsIMutI	CAGTG	3 cut(s)	12, 1036, 1362
Cac8I	GCNNGC	1 cut(s)	1153
Cfr13I	GGNCC	4 cut(s)	820, 1134, 1135, 1287
Csp6I	GTAC	4 cut(s)	526, 885, 1207, 1539
CviAII	CATG	6 cut(s)	579, 745, 824, 1049, 1254, 1485
CviQI	GTAC	4 cut(s)	526, 885, 1207, 1539
DdeI	CTNAG	2 cut(s)	633, 1119
DpnI	GATC	5 cut(s)	120, 387, 417, 672, 975
DpnII	GATC	5 cut(s)	118, 385, 415, 670, 973
DraIII	CACNNNGTG	1 cut(s)	353
DrdI	GACNNNNNNGTC	1 cut(s)	1019
DseDI	GACNNNNNNGTC	1 cut(s)	1019
EaeI	YGGCCR	3 cut(s)	826, 1033, 1402
Eam1104I	CTCTTC	5 cut(s)	83, 89, 169, 841, 1216
EarI	CTCTTC	5 cut(s)	83, 89, 169, 841, 1216
Eco130I	CCWWGG	3 cut(s)	677, 823, 840
Eco147I	AGGCCT	1 cut(s)	1124
Eco24I	GRGCYC	2 cut(s)	115, 1138
Eco32I	GATATC	1 cut(s)	837
Eco47I	GGWCC	1 cut(s)	820
Eco57I	CTGAAG	2 cut(s)	470, 1160
EcoO109I	RGGNCCY	1 cut(s)	1135
EcoRI	GAATTC	1 cut(s)	949
EcoRII	CCWGG	5 cut(s)	333, 870, 1145, 1436, 1468
EcoRV	GATATC	1 cut(s)	837
EcoT14I	CCWWGG	3 cut(s)	677, 823, 840
EcoT22I	ATGCAT	2 cut(s)	1117, 1486
EcoT38I	GRGCYC	2 cut(s)	115, 1138
ErhI	CCWWGG	3 cut(s)	677, 823, 840
FaeI	CATG	6 cut(s)	582, 748, 827, 1052, 1257, 1488
FalI	AAGNNNNNCTT	2 cut(s)	131, 163
FaqI	GGGAC	2 cut(s)	247, 1460
FatI	CATG	6 cut(s)	578, 744, 823, 1048, 1253, 1484
FauI	CCCGC	1 cut(s)	567
Fnu4HI	GCNGC	4 cut(s)	204, 207, 210, 1453
FokI	GGATG	2 cut(s)	1340, 1392
FriOI	GRGCYC	2 cut(s)	115, 1138
Fsp4HI	GCNGC	4 cut(s)	204, 207, 210, 1453
FspBI	CTAG	1 cut(s)	1152
GluI	GCNGC	4 cut(s)	204, 207, 210, 1453
GsaI	CCCAGC	1 cut(s)	1159
GsuI	CTGGAG	1 cut(s)	1491
HaeIII	GGCC	6 cut(s)	828, 1035, 1124, 1136, 1289, 1404
HapII	CCGG	2 cut(s)	18, 572
Hin1II	CATG	6 cut(s)	582, 748, 827, 1052, 1257, 1488
HincII	GTYRAC	1 cut(s)	1383
HindII	GTYRAC	1 cut(s)	1383
HindIII	AAGCTT	1 cut(s)	597
HpaII	CCGG	2 cut(s)	18, 572
HphI	GGTGA	8 cut(s)	64, 79, 94, 488, 518, 553, 715, 1274
Hpy166II	GTNNAC	3 cut(s)	347, 1044, 1383
Hpy188I	TCNGA	5 cut(s)	634, 753, 785, 1120, 1226
Hpy188III	TCNNGA	3 cut(s)	348, 389, 419
Hpy8I	GTNNAC	3 cut(s)	347, 1044, 1383
HpyCH4III	ACNGT	4 cut(s)	884, 889, 1421, 1543
HpyCH4IV	ACGT	2 cut(s)	528, 997
HpyCH4V	TGCA	9 cut(s)	278, 323, 376, 406, 689, 1115, 1257, 1452, 1484
HpyF10VI	GCNNNNNNNGC	6 cut(s)	56, 209, 284, 559, 1121, 1401
HpyF3I	CTNAG	2 cut(s)	633, 1119
HpySE526I	ACGT	2 cut(s)	528, 997
Hsp92II	CATG	6 cut(s)	582, 748, 827, 1052, 1257, 1488
Kzo9I	GATC	5 cut(s)	118, 385, 415, 670, 973
LmnI	GCTCC	1 cut(s)	312
Lsp1109I	GCAGC	4 cut(s)	190, 193, 196, 1464
LweI	GCATC	4 cut(s)	717, 1102, 1124, 1471
MaeI	CTAG	1 cut(s)	1152
MaeII	ACGT	2 cut(s)	528, 997
MaeIII	GTNAC	2 cut(s)	44, 1372
MalI	GATC	5 cut(s)	120, 387, 417, 672, 975
MboI	GATC	5 cut(s)	118, 385, 415, 670, 973
MflI	RGATCY	1 cut(s)	385
MhlI	GDGCHC	2 cut(s)	115, 1138
MlsI	TGGCCA	3 cut(s)	828, 1035, 1404
MluCI	AATT	9 cut(s)	22, 244, 510, 866, 893, 949, 978, 1106, 1387
MluNI	TGGCCA	3 cut(s)	828, 1035, 1404
MlyI	GAGTC	6 cut(s)	464, 623, 773, 936, 1039, 1482
MmeI	TCCRAC	2 cut(s)	980, 983
Mox20I	TGGCCA	3 cut(s)	828, 1035, 1404
Mph1103I	ATGCAT	2 cut(s)	1117, 1486
MscI	TGGCCA	3 cut(s)	828, 1035, 1404
MseI	TTAA	4 cut(s)	684, 936, 1026, 1167
MslI	CAYNNNNRTG	1 cut(s)	1461
Msp20I	TGGCCA	3 cut(s)	828, 1035, 1404
MspI	CCGG	2 cut(s)	18, 572
MspR9I	CCNGG	7 cut(s)	18, 335, 573, 872, 1147, 1438, 1470
MvaI	CCWGG	5 cut(s)	335, 872, 1147, 1438, 1470
MwoI	GCNNNNNNNGC	6 cut(s)	56, 209, 284, 559, 1121, 1401
NciI	CCSGG	2 cut(s)	18, 573
NcoI	CCATGG	1 cut(s)	823
NdeII	GATC	5 cut(s)	118, 385, 415, 670, 973
NheI	GCTAGC	1 cut(s)	1151
NlaIII	CATG	6 cut(s)	582, 748, 827, 1052, 1257, 1488
NlaIV	GGNNCC	6 cut(s)	339, 755, 1136, 1288, 1353, 1432
NmuCI	GTSAC	1 cut(s)	1372
NsiI	ATGCAT	2 cut(s)	1117, 1486
PceI	AGGCCT	1 cut(s)	1124
PfeI	GAWTC	4 cut(s)	740, 748, 1174, 1241
PfoI	TCCNGGA	2 cut(s)	870, 1468
PkrI	GCNGC	4 cut(s)	205, 208, 211, 1454
PleI	GAGTC	6 cut(s)	463, 623, 772, 935, 1039, 1481
PpsI	GAGTC	6 cut(s)	463, 623, 772, 935, 1039, 1481
Ppu21I	YACGTR	1 cut(s)	529
Psp1406I	AACGTT	1 cut(s)	997
Psp6I	CCWGG	5 cut(s)	333, 870, 1145, 1436, 1468
PspFI	CCCAGC	1 cut(s)	1155
PspGI	CCWGG	5 cut(s)	333, 870, 1145, 1436, 1468
PspN4I	GGNNCC	6 cut(s)	339, 755, 1136, 1288, 1353, 1432
PspOMI	GGGCCC	1 cut(s)	1134
PspPI	GGNCC	4 cut(s)	820, 1134, 1135, 1287
PsrI	GAACNNNNNNTAC	2 cut(s)	1414, 1446
PstI	CTGCAG	1 cut(s)	1454
PsuI	RGATCY	1 cut(s)	385
RsaI	GTAC	4 cut(s)	527, 886, 1208, 1540
RsaNI	GTAC	4 cut(s)	526, 885, 1207, 1539
RseI	CAYNNNNRTG	1 cut(s)	1461
SaqAI	TTAA	4 cut(s)	684, 936, 1026, 1167
SatI	GCNGC	4 cut(s)	204, 207, 210, 1453
Sau3AI	GATC	5 cut(s)	118, 385, 415, 670, 973
Sau96I	GGNCC	4 cut(s)	820, 1134, 1135, 1287
SchI	GAGTC	6 cut(s)	464, 623, 773, 936, 1039, 1482
ScrFI	CCNGG	7 cut(s)	18, 335, 573, 872, 1147, 1438, 1470
SduI	GDGCHC	2 cut(s)	115, 1138
SfaNI	GCATC	4 cut(s)	717, 1102, 1124, 1471
SfcI	CTRYAG	3 cut(s)	282, 1126, 1450
SinI	GGWCC	1 cut(s)	820
SmiMI	CAYNNNNRTG	1 cut(s)	1461
SmlI	CTYRAG	1 cut(s)	942
SmoI	CTYRAG	1 cut(s)	942
Sse9I	AATT	9 cut(s)	22, 244, 510, 866, 893, 949, 978, 1106, 1387
SseBI	AGGCCT	1 cut(s)	1124
SsiI	CCGC	4 cut(s)	560, 649, 931, 1393
SspI	AATATT	1 cut(s)	771
SspMI	CTAG	1 cut(s)	1152
StuI	AGGCCT	1 cut(s)	1124
StyD4I	CCNGG	7 cut(s)	16, 333, 571, 870, 1145, 1436, 1468
StyI	CCWWGG	3 cut(s)	677, 823, 840
TaaI	ACNGT	4 cut(s)	884, 889, 1421, 1543
TaiI	ACGT	2 cut(s)	531, 1000
TasI	AATT	9 cut(s)	22, 244, 510, 866, 893, 949, 978, 1106, 1387
TatI	WGTACW	1 cut(s)	1538
TfiI	GAWTC	4 cut(s)	740, 748, 1174, 1241
Tru1I	TTAA	4 cut(s)	684, 936, 1026, 1167
Tru9I	TTAA	4 cut(s)	684, 936, 1026, 1167
TscAI	CASTG	3 cut(s)	12, 1043, 1362
TseFI	GTSAC	1 cut(s)	1372
TseI	GCWGC	4 cut(s)	203, 206, 209, 1452
Tsp45I	GTSAC	1 cut(s)	1372
TspGWI	ACGGA	1 cut(s)	504
TspRI	CASTG	3 cut(s)	12, 1043, 1362
VpaK11BI	GGWCC	1 cut(s)	820
XapI	RAATTY	5 cut(s)	510, 866, 893, 949, 1106
XspI	CTAG	1 cut(s)	1152
Zsp2I	ATGCAT	2 cut(s)	1117, 1486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.