MD17G1023100.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006146
Physical Location & Seq
Reverse (-)
1696636 .. 1699235
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1023100.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGTGTTTAGTTTTCGTCCATGAACTCTCATATTTGTTTCTCTCGGCTTGGCTGATCAACAGTAGAGGACTTGCAAGCAAAGTGAAGAATGCTGCTCGGTCGACCGCTTGTCCGATCAAAGAGTGCGGGGCAAATCGTGAATGCCCGAATTGCCATTTTGTTATAGATAACAGCGATGTTTTATCGGAATGGCCTGGCTTACCGGTTGGTGTGAAGTTTGATCCATCTGATGGTGAACTCTTAGTACACTTAGCGGCTAAGTGTGGTGTCGGAAACTCACAGCCACACTTGCTTATCGATGAGTTCATCCCTACACTTGAGGGGGATAATGGAATTTGCTATGATCATCCCAAAAACCTTCCTGGCGCAACGAAAGATGGAAGCAGCATCCATTTCTTCCATAAAACAATGAAAGCATATGCTACTGGTCAGAGGAAGCGCCGCAAGGTTGATAGTCAGCGTAGCGTGGCTTCAGAGGATGTCCGCTGGCACAAAACGGGTAAGACCAAGGCTATAATGGAAAATGGAGTTAAGGGCTGGAAGAAGATCATGGTCCTATACAGAAGTTCGAAGAAAGGTATCAAACCAGAAAAAGTTAACTGGGTGATGCATCAGTATCACCTGGGAGCTGAAGAGGATGAGAGAGAAGGTGAATATGTGGTGTCAAAGATATTCTACCAGCAGCATAAGCAGTCAGAAAGGAATGACAACAATGTGGTCACTGAAGATAATGATGTCAATGCACTTCGAACTAGCCCCAGGACCCCCGTACCCAATCCTCCAATTCCACCTAGACCAGGGAAATCTGTATGCTTTGAGGATGTTCCTGATGAAGCTATGCTCCGCTCATCTGTCAAGGAACCGGAGCTTGCCCAGGAAGCATATCGTGTCCCTCAACCTGATACTCGGTTTGAAGACAATATTGGGCAGCCTACCTGGCTCGCAGGCGAATCTCAGGCCGACGATTTGCATAGCTTGGATGAAACCTTGTTATGCAAGGAGATCTTTGGTTCAGCAACGAACCTCATATCTAACACTGACTTCAATAACAATACATACGAGGTGAATGGAAACAGCAGCACATCGTGTGGACGTCATGACCTTGAGAATATTGGACTTGATTCTCCTCCAGATTTCCAGCTGCCTGATTTTCAGTTTTCATCCCAAGACAGTTTTAATTGGCTAGACAGGTTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

400

Amino Acids

44.76

Weight (kDa)

5.66

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 68 - 208 3.3e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1096
AccB7I CCANNNNNTGG 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 846
AccI GTMKAC 1 cut(s) 101
AciI CCGC 6 cut(s) 105, 126, 254, 442, 484, 844
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 3 cut(s) 456, 651, 744
AcyI GRCGYC 1 cut(s) 1093
AdeI CACNNNGTG 1 cut(s) 1086
AfaI GTAC 2 cut(s) 246, 771
AfiI CCNNNNNNNGG 2 cut(s) 230, 797
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 2 cut(s) 914, 1045
AhdI GACNNNNNGTC 1 cut(s) 108
AjnI CCWGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
AluBI AGCT 6 cut(s) 629, 836, 868, 975, 1141, 1197
AluI AGCT 6 cut(s) 629, 836, 868, 975, 1141, 1197
AlwI GGATC 1 cut(s) 215
AoxI GGCC 2 cut(s) 191, 957
ApeKI GCWGC 6 cut(s) 92, 384, 682, 928, 1077, 1141
ApoI RAATTY 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 2 cut(s) 368, 441
AspS9I GGNCC 2 cut(s) 553, 762
AsuHPI GGTGA 5 cut(s) 245, 611, 616, 662, 1075
AsuII TTCGAA 2 cut(s) 569, 748
AvaII GGWCC 2 cut(s) 553, 762
BaeI ACNNNNGTAYC 2 cut(s) 753, 786
BarI GAAGNNNNNNTAC 2 cut(s) 454, 486
BbsI GAAGAC 1 cut(s) 921
BbvI GCAGC 6 cut(s) 79, 396, 694, 940, 1089, 1128
BccI CCATC 3 cut(s) 224, 232, 371
BciT130I CCWGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
BclI TGATCA 2 cut(s) 54, 343
BfaI CTAG 3 cut(s) 753, 792, 1184
BfoI RGCGCY 1 cut(s) 442
BglI GCCNNNNNGGC 1 cut(s) 937
BglII AGATCT 1 cut(s) 1002
BisI GCNGC 8 cut(s) 93, 255, 385, 442, 683, 929, 1078, 1142
BlsI GCNGC 8 cut(s) 94, 256, 386, 443, 684, 930, 1079, 1143
Bme1390I CCNGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
Bme18I GGWCC 2 cut(s) 553, 762
BmeRI GACNNNNNGTC 1 cut(s) 108
BmgT120I GGNCC 2 cut(s) 553, 762
BmiI GGNNCC 2 cut(s) 764, 861
BmrFI CCNGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 3 cut(s) 396, 597, 619
BmuI ACTGGG 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 921
BpmI CTGGAG 1 cut(s) 1113
Bpu14I TTCGAA 2 cut(s) 569, 748
BpuEI CTTGAG 2 cut(s) 338, 1124
Bsa29I ATCGAT 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 1093
BsaJI CCNNGG 5 cut(s) 507, 622, 758, 797, 873
BsaWI WCCGGW 2 cut(s) 202, 862
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 797
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 430, 605
BseBI CCWGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 5 cut(s) 507, 622, 758, 797, 873
BseGI GGATG 8 cut(s) 306, 346, 387, 484, 643, 826, 985, 1160
BseLI CCNNNNNNNGG 2 cut(s) 230, 797
BseMII CTCAG 1 cut(s) 968
BseNI ACTGG 2 cut(s) 430, 605
BseRI GAGGAG 1 cut(s) 1116
BseXI GCAGC 6 cut(s) 79, 396, 694, 940, 1089, 1128
Bsh1285I CGRYCG 2 cut(s) 101, 105
BshFI GGCC 2 cut(s) 193, 959
BshTI ACCGGT 1 cut(s) 202
BshVI ATCGAT 1 cut(s) 297
BsiEI CGRYCG 2 cut(s) 101, 105
BsiSI CCGG 2 cut(s) 203, 863
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 2 cut(s) 230, 797
BsmFI GGGAC 1 cut(s) 875
BsmI GAATGC 2 cut(s) 94, 146
BsnI GGCC 2 cut(s) 193, 959
Bsp119I TTCGAA 2 cut(s) 569, 748
Bsp143I GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
BspACI CCGC 6 cut(s) 105, 126, 254, 442, 484, 844
BspANI GGCC 2 cut(s) 193, 959
BspCNI CTCAG 1 cut(s) 967
BspDI ATCGAT 1 cut(s) 297
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 2 cut(s) 764, 861
BspPI GGATC 1 cut(s) 215
BspT104I TTCGAA 2 cut(s) 569, 748
BsrBI CCGCTC 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 430, 605
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 5 cut(s) 507, 622, 758, 797, 873
BssMI GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
BssNI GRCGYC 1 cut(s) 1093
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
Bst4CI ACNGT 2 cut(s) 62, 1172
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 1093
BstBI TTCGAA 2 cut(s) 569, 748
BstC8I GCNNGC 5 cut(s) 76, 488, 870, 942, 946
BstDEI CTNAG 4 cut(s) 241, 250, 258, 954
BstENI CCTNNNNNAGG 1 cut(s) 795
BstF5I GGATG 8 cut(s) 306, 346, 387, 484, 643, 826, 985, 1160
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 2 cut(s) 368, 441
BstKTI GATC 6 cut(s) 57, 117, 223, 346, 549, 1005
BstMBI GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
BstMCI CGRYCG 2 cut(s) 101, 105
BstMWI GCNNNNNNNGC 5 cut(s) 150, 289, 688, 878, 937
BstNI CCWGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
BstSCI CCNGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
BstV1I GCAGC 6 cut(s) 79, 396, 694, 940, 1089, 1128
BstV2I GAAGAC 1 cut(s) 921
BstX2I RGATCY 1 cut(s) 1002
BstYI RGATCY 1 cut(s) 1002
Bsu15I ATCGAT 1 cut(s) 297
BsuRI GGCC 2 cut(s) 193, 959
BsuTUI ATCGAT 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 8 cut(s) 306, 346, 387, 484, 643, 826, 985, 1160
BtsIMutI CAGTG 2 cut(s) 720, 1035
Cac8I GCNNGC 5 cut(s) 76, 488, 870, 942, 946
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 2 cut(s) 368, 441
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 553, 762
ClaI ATCGAT 1 cut(s) 297
Csp6I GTAC 2 cut(s) 245, 770
CspAI ACCGGT 1 cut(s) 202
CviAII CATG 3 cut(s) 20, 550, 1097
CviQI GTAC 2 cut(s) 245, 770
DdeI CTNAG 4 cut(s) 241, 250, 258, 954
DpnI GATC 6 cut(s) 56, 116, 222, 345, 548, 1004
DpnII GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
DraIII CACNNNGTG 1 cut(s) 1086
DriI GACNNNNNGTC 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 627
Eam1105I GACNNNNNGTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 553, 762
Eco57I CTGAAG 3 cut(s) 456, 651, 744
EcoNI CCTNNNNNAGG 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 3 cut(s) 23, 553, 1100
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FaqI GGGAC 1 cut(s) 875
FatI CATG 3 cut(s) 19, 549, 1096
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 2 cut(s) 54, 343
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 8 cut(s) 93, 255, 385, 442, 683, 929, 1078, 1142
FokI GGATG 8 cut(s) 293, 333, 374, 491, 650, 833, 992, 1147
Fsp4HI GCNGC 8 cut(s) 93, 255, 385, 442, 683, 929, 1078, 1142
FspBI CTAG 3 cut(s) 753, 792, 1184
GlaI GCGC 2 cut(s) 367, 440
GluI GCNGC 8 cut(s) 93, 255, 385, 442, 683, 929, 1078, 1142
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 2 cut(s) 193, 959
HapII CCGG 2 cut(s) 203, 863
HhaI GCGC 2 cut(s) 368, 441
Hin1I GRCGYC 1 cut(s) 1093
Hin1II CATG 3 cut(s) 23, 553, 1100
Hin6I GCGC 2 cut(s) 366, 439
HinP1I GCGC 2 cut(s) 366, 439
HincII GTYRAC 2 cut(s) 102, 598
HindII GTYRAC 2 cut(s) 102, 598
HinfI GANTC 2 cut(s) 950, 1121
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 2 cut(s) 203, 863
HphI GGTGA 5 cut(s) 245, 611, 616, 662, 1075
Hpy166II GTNNAC 5 cut(s) 102, 236, 247, 598, 1091
Hpy188I TCNGA 7 cut(s) 114, 187, 229, 272, 432, 475, 697
Hpy188III TCNNGA 4 cut(s) 137, 827, 1097, 1130
Hpy8I GTNNAC 5 cut(s) 102, 236, 247, 598, 1091
Hpy99I CGWCG 1 cut(s) 965
HpyAV CCTTC 2 cut(s) 368, 641
HpyCH4III ACNGT 2 cut(s) 62, 1172
HpyCH4IV ACGT 1 cut(s) 1093
HpyCH4V TGCA 5 cut(s) 74, 610, 743, 970, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 289, 688, 878, 937
HpyF3I CTNAG 4 cut(s) 241, 250, 258, 954
HpySE526I ACGT 1 cut(s) 1093
Hsp92I GRCGYC 1 cut(s) 1093
Hsp92II CATG 3 cut(s) 23, 553, 1100
HspAI GCGC 2 cut(s) 366, 439
Ksp22I TGATCA 2 cut(s) 54, 343
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
LmnI GCTCC 3 cut(s) 626, 846, 865
Lsp1109I GCAGC 6 cut(s) 79, 396, 694, 940, 1089, 1128
LweI GCATC 3 cut(s) 396, 597, 619
MaeI CTAG 3 cut(s) 753, 792, 1184
MaeII ACGT 1 cut(s) 1093
MaeIII GTNAC 1 cut(s) 718
MalI GATC 6 cut(s) 56, 116, 222, 345, 548, 1004
MbiI CCGCTC 1 cut(s) 846
MboI GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
MboII GAAGA 8 cut(s) 97, 388, 553, 556, 583, 644, 737, 926
MflI RGATCY 1 cut(s) 1002
MluCI AATT 4 cut(s) 148, 333, 783, 1177
MmeI TCCRAC 1 cut(s) 250
Mph1103I ATGCAT 1 cut(s) 612
MseI TTAA 4 cut(s) 531, 597, 1176, 1193
MspA1I CMGCKG 2 cut(s) 486, 1141
MspI CCGG 2 cut(s) 203, 863
MspR9I CCNGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
Mva1269I GAATGC 2 cut(s) 94, 146
MvaI CCWGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
MwoI GCNNNNNNNGC 5 cut(s) 150, 289, 688, 878, 937
NdeI CATATG 1 cut(s) 418
NdeII GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
NlaIII CATG 3 cut(s) 23, 553, 1100
NlaIV GGNNCC 2 cut(s) 764, 861
NmeAIII GCCGAG 1 cut(s) 23
NmuCI GTSAC 1 cut(s) 718
NsiI ATGCAT 1 cut(s) 612
NspV TTCGAA 2 cut(s) 569, 748
PagI TCATGA 1 cut(s) 1096
PctI GAATGC 2 cut(s) 94, 146
PfeI GAWTC 2 cut(s) 950, 1121
PflMI CCANNNNNTGG 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 8 cut(s) 94, 256, 386, 443, 684, 930, 1079, 1143
PpuMI RGGWCCY 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 762
Psp6I CCWGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
PspGI CCWGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
PspN4I GGNNCC 2 cut(s) 764, 861
PspPI GGNCC 2 cut(s) 553, 762
PspPPI RGGWCCY 1 cut(s) 762
PsrI GAACNNNNNNTAC 2 cut(s) 228, 260
PsuI RGATCY 1 cut(s) 1002
PvuII CAGCTG 1 cut(s) 1141
RsaI GTAC 2 cut(s) 246, 771
RsaNI GTAC 2 cut(s) 245, 770
SalI GTCGAC 1 cut(s) 100
SaqAI TTAA 4 cut(s) 531, 597, 1176, 1193
SatI GCNGC 8 cut(s) 93, 255, 385, 442, 683, 929, 1078, 1142
Sau3AI GATC 6 cut(s) 54, 114, 220, 343, 546, 1002
Sau96I GGNCC 2 cut(s) 553, 762
ScrFI CCNGG 7 cut(s) 195, 363, 623, 760, 798, 875, 937
SfaNI GCATC 3 cut(s) 396, 597, 619
SfuI TTCGAA 2 cut(s) 569, 748
SinI GGWCC 2 cut(s) 553, 762
SmlI CTYRAG 2 cut(s) 317, 1103
SmoI CTYRAG 2 cut(s) 317, 1103
Sse9I AATT 4 cut(s) 148, 333, 783, 1177
SsiI CCGC 6 cut(s) 105, 126, 254, 442, 484, 844
SspI AATATT 2 cut(s) 922, 1111
SspMI CTAG 3 cut(s) 753, 792, 1184
StyD4I CCNGG 7 cut(s) 193, 361, 621, 758, 796, 873, 935
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 2 cut(s) 62, 1172
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 4 cut(s) 101, 297, 569, 748
TaqII GACCGA 1 cut(s) 87
TasI AATT 4 cut(s) 148, 333, 783, 1177
TatI WGTACW 1 cut(s) 244
TauI GCSGC 2 cut(s) 257, 444
TfiI GAWTC 2 cut(s) 950, 1121
Tru1I TTAA 4 cut(s) 531, 597, 1176, 1193
Tru9I TTAA 4 cut(s) 531, 597, 1176, 1193
TscAI CASTG 2 cut(s) 727, 1042
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 6 cut(s) 92, 384, 682, 928, 1077, 1141
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 6 cut(s) 36, 295, 425, 846, 996, 1149
TspRI CASTG 2 cut(s) 727, 1042
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 553, 762
XagI CCTNNNNNAGG 1 cut(s) 795
XapI RAATTY 1 cut(s) 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 101
XspI CTAG 3 cut(s) 753, 792, 1184
ZraI GACGTC 1 cut(s) 1094
Zsp2I ATGCAT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.