Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005798

Physical Location & Seq

Genomic Coordinates Forward (+)

1980788 .. 1984230

Gene Structure 3443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1027700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGGAGATCTCAAAGCCCCAATCGTTCTTCCAAGACATCAGATTCAGAGAGCTCCACGGATTCAGAGCTCGGAAGCGGCCGTACGTCAATGGACTAGCATCGGATTTCTACGAAATTGGCGCCATTGCTGCAGAGCACAACGGCCCTCCGATGGCAATCTCGTTCGGCAAGACTTGGAAAAGCTCACACATCGTCGCCATGTCCGATGAGGATGGGTACTTGAGCCTGTTCAATACACGGCGCAAATTCTGTGCATCTGCATCTCACCAAGAAAATGCAGATAAAACTAAGGTTTGCGATTGGGTTGCACATCATAACACTGTGTTCGATATCTGTTGGATTAAGGATGATACTCAAATTATGACAGCTTCCGGCGATCAGACTATCAAGGTATGGGATATTCAGGAAAAGAAATGTACCGCTGTACTGATGGGGCACACAGGAAGTGTGAAATCTCTGTGTCCTCATCCAACTAATCCTGAGGTTATAGTCTCTGGTTCGAGAGATGGCTCCTTTGCTCTTTGGGATATGAGATGTAATTCAAGCTCCAAAAATATGCAGGGGGAGGTTTCTATATGCTCAACTGCAGTGGTTACAGGGGCTCATATTGTTCCACGGGCAAAGCGTGTAAGGCGTGGAAAGGCTGTTTCCATGAGCATCACATCAGTTCTTTATCTTAAGGATGAGGTCTCCATTGCTACCGCCGGAGCTGTGGACAGCATTGTCAAGTTTTGGGATACCAGAAGTTTGAAAAATGTTGTCACACAGACATGCCCTCAACCAGAGTCAACTGAGAAGGAAAGACGATTACACGGTATAACTAGCTTATCCCAAGATTTAAATGGAGTGTTTCTTTCAGCGTCATGCATGGACAACAGCATATATTTGTATAACGTACTTCAGCTACAAAAGGGTCCTATGCAAGCGTTCACCGGATGCCGGATTGAAACATTTTTTGTTAAGTCAGTTATTAGCCCCGATGCTGCTCGCATAGTCAGTGGCTCGAGCGATGGAAATGCCTATATTTGGCAGGTAAACAAGCCTCAGGAGGATCCCGTTGTATTGAAAGGCCATGACAGGGAAGTTACAGCAGTAGAGTGGTCCCAGTTTGAGGTCGGGAAGATGGCGACGTCGTCAGATGATTTTACGGTATGCAAGACAGAGTCACAGTTCATATATAAGATTGTACATTCATCAATTCACACAATGCATTTGCCATTTTCTATAACGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0000151 GO:0000209 GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010564 GO:0010948 GO:0015630 GO:0016567 GO:0018130 GO:0019438 GO:0019538 GO:0019941 GO:0019985 GO:0023052 GO:0030163 GO:0031461 GO:0031464 GO:0031465 GO:0031570 GO:0031572 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0071897 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080008 GO:0090304 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901987 GO:1901988 GO:1902494 GO:1902749 GO:1902750 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

412

Amino Acids

45.76

Weight (kDa)

7.97

Isoelectric Point (pI)

37.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	58 - 197	7.6e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	59 - 195	6.6e-13	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	61 - 186	7.5e-11	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	83 - 191	1.4e-14	G protein beta WD-40 repeat protein
EIF3I	PF24805	87 - 199	1.7e-06	EIF3I
WDR55	PF24796	91 - 188	2.1e-08	WDR55
WD40_Prp19	PF24814	97 - 190	4.2e-14	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	97 - 189	4.6e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	102 - 177	1.3e-08	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	104 - 195	1.8e-17	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	106 - 188	5.6e-12	WDHD1 first WD40 domain
Beta-prop_WDR75_1st	PF23869	116 - 194	1.9e-06	WD repeat-containing protein 75 first beta-propeller
Beta-prop_SCAP	PF24017	119 - 203	9.9e-07	SCAP Beta-propeller
WD40	PF00400	138 - 176	6.2e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	208 - 369	6.1e-06	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	211 - 378	1.7e-11	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	217 - 378	2.6e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	270 - 384	1.6e-08	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	325 - 384	5e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27640 AT3G27640
fragaria_vesca	FvH4_6g51280 FvH4_6g51280 FvH4_6g51280 FvH4_6g51280
malus_domestica	MD09G1027800.v1.1 MD17G1027700.v1.1 MD17G1028200.v1.1
prunus_persica	Prupe.3G290400_v2.0.a1 Prupe.3G291000_v2.0.a1
pyrus_communis	pycom111g02180
rosa_chinensis	RchiOBHm_Chr2g0172901
rosa_laevigata	RLG00000022140
rosa_multiflora	Rmu_co8216202.1_g000001 Rmu_sc0002979.1_g000002 Rmu_sc0013380.1_g000001 Rmu_sc0015618.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079450
rosa_rugosa	Rorug02G0563200 Rorug02G0563400
rosa_samantha	Rh2AG640100 Rh2AG640200 Rh2BG653600 Rh2BG653700 Rh2CG619200 Rh2DG667200
rosa_wichuraiana	Rw2G052840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	722
AatII	GACGTC	1 cut(s)	1133
Acc36I	ACCTGC	1 cut(s)	1021
AccB1I	GGYRCC	1 cut(s)	119
AciI	CCGC	3 cut(s)	76, 420, 702
AclI	AACGTT	1 cut(s)	1229
AclWI	GGATC	2 cut(s)	1046, 1059
AcoI	YGGCCR	1 cut(s)	77
AcsI	RAATTY	1 cut(s)	245
AcuI	CTGAAG	1 cut(s)	884
AcyI	GRCGYC	2 cut(s)	120, 1130
AfaI	GTAC	6 cut(s)	83, 218, 418, 426, 897, 1188
AfiI	CCNNNNNNNGG	5 cut(s)	151, 939, 1026, 1078, 1111
AflII	CTTAAG	1 cut(s)	677
AgsI	TTSAA	5 cut(s)	232, 543, 751, 947, 1066
AluBI	AGCT	8 cut(s)	52, 68, 183, 368, 546, 710, 825, 904
AluI	AGCT	8 cut(s)	52, 68, 183, 368, 546, 710, 825, 904
Alw21I	GWGCWC	3 cut(s)	54, 70, 138
Alw26I	GTCTC	2 cut(s)	496, 694
AlwI	GGATC	2 cut(s)	1046, 1059
Ama87I	CYCGRG	1 cut(s)	1003
AoxI	GGCC	3 cut(s)	77, 142, 1069
ApeKI	GCWGC	2 cut(s)	128, 983
ApoI	RAATTY	1 cut(s)	245
AspLEI	GCGC	2 cut(s)	122, 243
AspS9I	GGNCC	3 cut(s)	143, 914, 1101
AsuHPI	GGTGA	2 cut(s)	257, 922
AvaI	CYCGRG	1 cut(s)	1003
AvaII	GGWCC	2 cut(s)	914, 1101
AxyI	CCTNAGG	2 cut(s)	480, 1044
BaeGI	GKGCMC	1 cut(s)	438
BamHI	GGATCC	1 cut(s)	1051
BanI	GGYRCC	1 cut(s)	119
BanII	GRGCYC	3 cut(s)	54, 70, 604
BarI	GAAGNNNNNNTAC	2 cut(s)	65, 97
Bbv12I	GWGCWC	3 cut(s)	54, 70, 138
BbvI	GCAGC	2 cut(s)	115, 970
BccI	CCATC	6 cut(s)	145, 206, 424, 500, 1004, 1117
BceAI	ACGGC	3 cut(s)	64, 157, 254
BcgI	CGANNNNNNTGC	4 cut(s)	287, 321, 998, 1032
BciVI	GTATCC	1 cut(s)	730
BcoDI	GTCTC	2 cut(s)	496, 694
BfaI	CTAG	2 cut(s)	95, 822
BfmI	CTRYAG	2 cut(s)	129, 585
BfoI	RGCGCY	1 cut(s)	123
BfrI	CTTAAG	1 cut(s)	677
BfuAI	ACCTGC	1 cut(s)	1021
BfuI	GTATCC	1 cut(s)	730
BglII	AGATCT	1 cut(s)	6
BisI	GCNGC	3 cut(s)	77, 129, 984
BlsI	GCNGC	3 cut(s)	78, 130, 985
Bme18I	GGWCC	2 cut(s)	914, 1101
BmeT110I	CYCGRG	1 cut(s)	1003
BmgT120I	GGNCC	3 cut(s)	143, 914, 1101
BmiI	GGNNCC	5 cut(s)	121, 511, 915, 1053, 1103
BmrI	ACTGGG	1 cut(s)	1099
BmsI	GCATC	6 cut(s)	107, 263, 269, 666, 926, 970
BmuI	ACTGGG	1 cut(s)	1099
BpuEI	CTTGAG	1 cut(s)	241
BsaBI	GATNNNNATC	1 cut(s)	155
BsaHI	GRCGYC	2 cut(s)	120, 1130
BsaI	GGTCTC	1 cut(s)	694
BsaJI	CCNNGG	2 cut(s)	55, 614
BsaWI	WCCGGW	1 cut(s)	932
Bsc4I	CCNNNNNNNGG	5 cut(s)	151, 939, 1026, 1078, 1111
Bse1I	ACTGG	1 cut(s)	1105
Bse21I	CCTNAGG	2 cut(s)	480, 1044
Bse3DI	GCAATG	2 cut(s)	123, 693
Bse8I	GATNNNNATC	1 cut(s)	155
BseDI	CCNNGG	2 cut(s)	55, 614
BseGI	GGATG	5 cut(s)	217, 352, 466, 688, 941
BseJI	GATNNNNATC	1 cut(s)	155
BseLI	CCNNNNNNNGG	5 cut(s)	151, 939, 1026, 1078, 1111
BseMI	GCAATG	2 cut(s)	123, 693
BseMII	CTCAG	3 cut(s)	471, 783, 1058
BseNI	ACTGG	1 cut(s)	1105
BseSI	GKGCMC	1 cut(s)	438
BseX3I	CGGCCG	1 cut(s)	77
BseXI	GCAGC	2 cut(s)	115, 970
Bsh1285I	CGRYCG	1 cut(s)	80
BshFI	GGCC	3 cut(s)	79, 144, 1071
BshNI	GGYRCC	1 cut(s)	119
BsiEI	CGRYCG	1 cut(s)	80
BsiHKAI	GWGCWC	3 cut(s)	54, 70, 138
BsiHKCI	CYCGRG	1 cut(s)	1003
BsiSI	CCGG	4 cut(s)	372, 705, 933, 940
BsiWI	CGTACG	1 cut(s)	81
BslFI	GGGAC	1 cut(s)	1087
BslI	CCNNNNNNNGG	5 cut(s)	151, 939, 1026, 1078, 1111
BsmAI	GTCTC	2 cut(s)	496, 694
BsmFI	GGGAC	1 cut(s)	1087
BsnI	GGCC	3 cut(s)	79, 144, 1071
Bso31I	GGTCTC	1 cut(s)	694
BsoBI	CYCGRG	1 cut(s)	1003
Bsp1286I	GDGCHC	5 cut(s)	54, 70, 138, 438, 604
Bsp1407I	TGTACA	1 cut(s)	1186
Bsp143I	GATC	3 cut(s)	6, 376, 1051
BspACI	CCGC	3 cut(s)	76, 420, 702
BspANI	GGCC	3 cut(s)	79, 144, 1071
BspCNI	CTCAG	3 cut(s)	472, 784, 1057
BspLI	GGNNCC	5 cut(s)	121, 511, 915, 1053, 1103
BspMAI	CTGCAG	2 cut(s)	133, 589
BspMI	ACCTGC	1 cut(s)	1021
BspPI	GGATC	2 cut(s)	1046, 1059
BspT107I	GGYRCC	1 cut(s)	119
BspTI	CTTAAG	1 cut(s)	677
BspTNI	GGTCTC	1 cut(s)	694
BsrDI	GCAATG	2 cut(s)	123, 693
BsrGI	TGTACA	1 cut(s)	1186
BsrI	ACTGG	1 cut(s)	1105
BssECI	CCNNGG	2 cut(s)	55, 614
BssMI	GATC	3 cut(s)	6, 376, 1051
BssNI	GRCGYC	2 cut(s)	120, 1130
Bst4CI	ACNGT	4 cut(s)	322, 815, 1150, 1170
BstACI	GRCGYC	2 cut(s)	120, 1130
BstAFI	CTTAAG	1 cut(s)	677
BstAUI	TGTACA	1 cut(s)	1186
BstC8I	GCNNGC	2 cut(s)	924, 988
BstDEI	CTNAG	4 cut(s)	288, 480, 792, 1044
BstDSI	CCRYGG	2 cut(s)	55, 614
BstF5I	GGATG	5 cut(s)	217, 352, 466, 688, 941
BstH2I	RGCGCY	1 cut(s)	123
BstHHI	GCGC	2 cut(s)	122, 243
BstKTI	GATC	3 cut(s)	9, 379, 1054
BstMAI	GTCTC	2 cut(s)	496, 694
BstMBI	GATC	3 cut(s)	6, 376, 1051
BstMCI	CGRYCG	1 cut(s)	80
BstMWI	GCNNNNNNNGC	2 cut(s)	128, 631
BstNSI	RCATGY	1 cut(s)	774
BstSFI	CTRYAG	2 cut(s)	129, 585
BstSLI	GKGCMC	1 cut(s)	438
BstV1I	GCAGC	2 cut(s)	115, 970
BstX2I	RGATCY	2 cut(s)	6, 1051
BstYI	RGATCY	2 cut(s)	6, 1051
BstZI	CGGCCG	1 cut(s)	77
Bsu36I	CCTNAGG	2 cut(s)	480, 1044
BsuI	GTATCC	1 cut(s)	730
BsuRI	GGCC	3 cut(s)	79, 144, 1071
BtgI	CCRYGG	2 cut(s)	55, 614
BtgZI	GCGATG	1 cut(s)	1023
BtsCI	GGATG	5 cut(s)	217, 352, 466, 688, 941
BtsI	GCAGTG	1 cut(s)	594
BtsIMutI	CAGTG	3 cut(s)	318, 594, 1003
BveI	ACCTGC	1 cut(s)	1021
Cac8I	GCNNGC	2 cut(s)	924, 988
CfoI	GCGC	2 cut(s)	122, 243
Cfr13I	GGNCC	3 cut(s)	143, 914, 1101
CseI	GACGC	1 cut(s)	849
Csp6I	GTAC	6 cut(s)	82, 217, 417, 425, 896, 1187
CspCI	CAANNNNNGTGG	2 cut(s)	570, 605
CviAII	CATG	6 cut(s)	199, 652, 771, 864, 868, 1073
CviQI	GTAC	6 cut(s)	82, 217, 417, 425, 896, 1187
DdeI	CTNAG	4 cut(s)	288, 480, 792, 1044
DinI	GGCGCC	1 cut(s)	121
DpnI	GATC	3 cut(s)	8, 378, 1053
DpnII	GATC	3 cut(s)	6, 376, 1051
DraI	TTTAAA	1 cut(s)	840
DrdI	GACNNNNNNGTC	1 cut(s)	722
DseDI	GACNNNNNNGTC	1 cut(s)	722
EaeI	YGGCCR	1 cut(s)	77
EagI	CGGCCG	1 cut(s)	77
Ecl136II	GAGCTC	2 cut(s)	52, 68
EclXI	CGGCCG	1 cut(s)	77
Eco24I	GRGCYC	3 cut(s)	54, 70, 604
Eco31I	GGTCTC	1 cut(s)	694
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	2 cut(s)	914, 1101
Eco52I	CGGCCG	1 cut(s)	77
Eco53kI	GAGCTC	2 cut(s)	52, 68
Eco57I	CTGAAG	1 cut(s)	884
Eco81I	CCTNAGG	2 cut(s)	480, 1044
Eco88I	CYCGRG	1 cut(s)	1003
EcoICRI	GAGCTC	2 cut(s)	52, 68
EcoO109I	RGGNCCY	1 cut(s)	914
EcoRV	GATATC	1 cut(s)	331
EcoT22I	ATGCAT	2 cut(s)	869, 1212
EcoT38I	GRGCYC	3 cut(s)	54, 70, 604
EgeI	GGCGCC	1 cut(s)	121
EheI	GGCGCC	1 cut(s)	121
FaeI	CATG	6 cut(s)	202, 655, 774, 867, 871, 1076
FaqI	GGGAC	1 cut(s)	1087
FatI	CATG	6 cut(s)	198, 651, 770, 863, 867, 1072
Fnu4HI	GCNGC	3 cut(s)	77, 129, 984
FokI	GGATG	5 cut(s)	224, 359, 453, 695, 948
FriOI	GRGCYC	3 cut(s)	54, 70, 604
Fsp4HI	GCNGC	3 cut(s)	77, 129, 984
FspBI	CTAG	2 cut(s)	95, 822
GlaI	GCGC	2 cut(s)	121, 242
GluI	GCNGC	3 cut(s)	77, 129, 984
HaeII	RGCGCY	1 cut(s)	123
HaeIII	GGCC	3 cut(s)	79, 144, 1071
HapII	CCGG	4 cut(s)	372, 705, 933, 940
HgaI	GACGC	1 cut(s)	849
HhaI	GCGC	2 cut(s)	122, 243
Hin1I	GRCGYC	2 cut(s)	120, 1130
Hin1II	CATG	6 cut(s)	202, 655, 774, 867, 871, 1076
Hin6I	GCGC	2 cut(s)	120, 241
HinP1I	GCGC	2 cut(s)	120, 241
HincII	GTYRAC	1 cut(s)	789
HindII	GTYRAC	1 cut(s)	789
HinfI	GANTC	4 cut(s)	42, 60, 785, 1163
HpaII	CCGG	4 cut(s)	372, 705, 933, 940
HphI	GGTGA	2 cut(s)	257, 922
Hpy166II	GTNNAC	4 cut(s)	715, 789, 930, 1036
Hpy188I	TCNGA	9 cut(s)	41, 47, 65, 72, 103, 150, 205, 381, 1138
Hpy188III	TCNNGA	5 cut(s)	404, 479, 501, 1046, 1117
Hpy8I	GTNNAC	4 cut(s)	715, 789, 930, 1036
Hpy99I	CGWCG	3 cut(s)	197, 1132, 1135
HpyAV	CCTTC	1 cut(s)	790
HpyCH4III	ACNGT	4 cut(s)	322, 815, 1150, 1170
HpyCH4IV	ACGT	4 cut(s)	84, 894, 1130, 1229
HpyF10VI	GCNNNNNNNGC	2 cut(s)	128, 631
HpyF3I	CTNAG	4 cut(s)	288, 480, 792, 1044
HpySE526I	ACGT	4 cut(s)	84, 894, 1130, 1229
Hsp92I	GRCGYC	2 cut(s)	120, 1130
Hsp92II	CATG	6 cut(s)	202, 655, 774, 867, 871, 1076
HspAI	GCGC	2 cut(s)	120, 241
KasI	GGCGCC	1 cut(s)	119
Kzo9I	GATC	3 cut(s)	6, 376, 1051
LmnI	GCTCC	4 cut(s)	57, 515, 551, 707
Lsp1109I	GCAGC	2 cut(s)	115, 970
LweI	GCATC	6 cut(s)	107, 263, 269, 666, 926, 970
MaeI	CTAG	2 cut(s)	95, 822
MaeII	ACGT	4 cut(s)	84, 894, 1130, 1229
MaeIII	GTNAC	4 cut(s)	592, 760, 1084, 1164
MalI	GATC	3 cut(s)	8, 378, 1053
MboI	GATC	3 cut(s)	6, 376, 1051
MboII	GAAGA	2 cut(s)	19, 1132
MflI	RGATCY	2 cut(s)	6, 1051
MhlI	GDGCHC	5 cut(s)	54, 70, 138, 438, 604
MluCI	AATT	5 cut(s)	114, 245, 357, 538, 1197
Mly113I	GGCGCC	1 cut(s)	120
MlyI	GAGTC	2 cut(s)	794, 1172
MmeI	TCCRAC	2 cut(s)	317, 494
Mph1103I	ATGCAT	2 cut(s)	869, 1212
MseI	TTAA	4 cut(s)	342, 678, 839, 960
MslI	CAYNNNNRTG	1 cut(s)	769
MspA1I	CMGCKG	1 cut(s)	422
MspCI	CTTAAG	1 cut(s)	677
MspI	CCGG	4 cut(s)	372, 705, 933, 940
MwoI	GCNNNNNNNGC	2 cut(s)	128, 631
NarI	GGCGCC	1 cut(s)	120
NdeII	GATC	3 cut(s)	6, 376, 1051
NlaIII	CATG	6 cut(s)	202, 655, 774, 867, 871, 1076
NlaIV	GGNNCC	5 cut(s)	121, 511, 915, 1053, 1103
NmuCI	GTSAC	2 cut(s)	760, 1164
NsiI	ATGCAT	2 cut(s)	869, 1212
NspI	RCATGY	1 cut(s)	774
PaeR7I	CTCGAG	1 cut(s)	1003
PcsI	WCGNNNNNNNCGW	2 cut(s)	201, 622
PfeI	GAWTC	2 cut(s)	42, 60
Pfl23II	CGTACG	1 cut(s)	81
PflFI	GACNNNGTC	2 cut(s)	1132, 1162
PkrI	GCNGC	3 cut(s)	78, 130, 985
PleI	GAGTC	2 cut(s)	793, 1171
PluTI	GGCGCC	1 cut(s)	123
PpsI	GAGTC	2 cut(s)	793, 1171
PpuMI	RGGWCCY	1 cut(s)	914
Psp124BI	GAGCTC	2 cut(s)	54, 70
Psp1406I	AACGTT	1 cut(s)	1229
Psp5II	RGGWCCY	1 cut(s)	914
PspLI	CGTACG	1 cut(s)	81
PspN4I	GGNNCC	5 cut(s)	121, 511, 915, 1053, 1103
PspPI	GGNCC	3 cut(s)	143, 914, 1101
PspPPI	RGGWCCY	1 cut(s)	914
PspXI	VCTCGAGB	1 cut(s)	1003
PstI	CTGCAG	2 cut(s)	133, 589
PsuI	RGATCY	2 cut(s)	6, 1051
PsyI	GACNNNGTC	2 cut(s)	1132, 1162
RsaI	GTAC	6 cut(s)	83, 218, 418, 426, 897, 1188
RsaNI	GTAC	6 cut(s)	82, 217, 417, 425, 896, 1187
RseI	CAYNNNNRTG	1 cut(s)	769
SacI	GAGCTC	2 cut(s)	54, 70
SaqAI	TTAA	4 cut(s)	342, 678, 839, 960
SatI	GCNGC	3 cut(s)	77, 129, 984
Sau3AI	GATC	3 cut(s)	6, 376, 1051
Sau96I	GGNCC	3 cut(s)	143, 914, 1101
SchI	GAGTC	2 cut(s)	794, 1172
SduI	GDGCHC	5 cut(s)	54, 70, 138, 438, 604
SfaNI	GCATC	6 cut(s)	107, 263, 269, 666, 926, 970
SfcI	CTRYAG	2 cut(s)	129, 585
SfoI	GGCGCC	1 cut(s)	121
Sfr274I	CTCGAG	1 cut(s)	1003
SinI	GGWCC	2 cut(s)	914, 1101
SlaI	CTCGAG	1 cut(s)	1003
SmiI	ATTTAAAT	1 cut(s)	840
SmiMI	CAYNNNNRTG	1 cut(s)	769
SmlI	CTYRAG	3 cut(s)	220, 677, 1003
SmoI	CTYRAG	3 cut(s)	220, 677, 1003
Sse9I	AATT	5 cut(s)	114, 245, 357, 538, 1197
SsiI	CCGC	3 cut(s)	76, 420, 702
SspDI	GGCGCC	1 cut(s)	119
SspMI	CTAG	2 cut(s)	95, 822
SstI	GAGCTC	2 cut(s)	54, 70
SwaI	ATTTAAAT	1 cut(s)	840
TaaI	ACNGT	4 cut(s)	322, 815, 1150, 1170
TaiI	ACGT	4 cut(s)	87, 897, 1133, 1232
TaqI	TCGA	3 cut(s)	327, 500, 1004
TasI	AATT	5 cut(s)	114, 245, 357, 538, 1197
TatI	WGTACW	2 cut(s)	424, 1186
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	2 cut(s)	42, 60
Tru1I	TTAA	4 cut(s)	342, 678, 839, 960
Tru9I	TTAA	4 cut(s)	342, 678, 839, 960
TscAI	CASTG	3 cut(s)	325, 594, 1003
TseFI	GTSAC	2 cut(s)	760, 1164
TseI	GCWGC	2 cut(s)	128, 983
Tsp45I	GTSAC	2 cut(s)	760, 1164
TspDTI	ATGAA	2 cut(s)	1162, 1182
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	3 cut(s)	325, 594, 1003
Tth111I	GACNNNGTC	2 cut(s)	1132, 1162
Vha464I	CTTAAG	1 cut(s)	677
VpaK11BI	GGWCC	2 cut(s)	914, 1101
XapI	RAATTY	1 cut(s)	245
XceI	RCATGY	1 cut(s)	774
XcmI	CCANNNNNNNNNTGG	1 cut(s)	839
XhoI	CTCGAG	1 cut(s)	1003
XspI	CTAG	2 cut(s)	95, 822
ZraI	GACGTC	1 cut(s)	1131
Zsp2I	ATGCAT	2 cut(s)	869, 1212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.