Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0005798

Physical Location & Seq

Genomic Coordinates Forward (+)

1311958 .. 1315632

Gene Structure 3675 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom111g02180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGAGATCTCAAAGCCCCAATCGTTCTTCCAAGACATCAGATCTAGAGAGCTTCACGGATTCAGAGCTCGGAAGCGGCCGTACATCAATGGCTTGGCATCAGATTTCTACGAAATTGGCGCCATCGCTGCAGAGCACAACGGCCCTCCGATGGCGATTTCGTTCGGCAAGACTTGGAAAAGCTCGCACATAGTCGCCATGTCCGACGAGGATGGGTACTTGAGCCTGTTCGATACACGTCGCAAGTTCTCCGCATCTGCATCTCACCAAGAAAACGCAGATAAAACTAGGGTTTGTGATTGGGTTGCGCATCAAAACACTGTGTTTGATATCTGTTGGATTAAGGATGACACTCAAATTATGACAGCTTCTGGTGATCAGACTATCAAGGTATGGGATATTCAGGAAAAGAAATGTACTGCATTACTGATGGGGCACACAGGAAGTGTGAAATCTCTTTGTCCTCATCCAACTAATCCCGAGATTATTGTCTCTGGTTCGAGAGATGGCTCCTTTGCTCTTTGGGATATGAGATGTAATTCAAGCTCCACAAATATGCAGGGGGAGCTTTCTATACGCTCAACTGCCGTGGTTACAGGGGCTCATATTTTTCCACGGGCAAAACGTGTAAGGCGTGGAAAGCTTTTACAGGCTGTTTCCATGAGCATCACGTCAGTTCTTTATCTTAAAGATGAGGTCTCCATTGCTACTGCCGGAGCTGTGGACAGCATTGTCAAGTTTTGGGATACCAGAAGTTTGAAAAATGTTGTCACACAGACATGCCCTCAACCAGAGTCAACTGAGAAGGAAAGACGATTACACGGTATAACTAGCTTATCCCAAGATCTAAATGGAGTGTTTCTTTCAGCTTCATGCATGGATAACAGCATATATTTGTATAATGTACTTCAGCTAGAAAAGGGTGCTATGCAAACGTTCACTGGATGCCGGATTGAAACTTTTTTTGTTAAGTCAGTTATTAGTCCTGATGCTGCTCAAATACTCAGTGGCTCTAGCGATGGAAATGCCTATATTTGGCAGGTAAACAAGCCTCAGGAAGACCCCATTATATTGAAAGGCCACGACAGGGAAGTTACTGCAGTGGATTGGTCCCAGTTTGAGGTTGGGAAGATGGCAACTTCGTCAGATGATTTTACGGTTCGCATTTGGAACTCTCAGAATAGTGACTCCCCAAGCACAAGCTCTCCATCTACCATTCGAAGGAGAGTCATGGCAATCCCAAGTGCAGAGTGTAGAAAGCTTCTGATGAATGAATCAACAATGCATACCTCAAAGGATTCCGGAAATCTGTGTCCATCAGATGAAGGATTAGACAAAACTAACTCACTCAATCCGATTAGTATGCATAAAATGAGTACTCCCCCCTCTCAAAAGAAACAATCTACGTCGGATCCTGACTTTAGCAAAACCTTGGAGAAGACCCCCGAAGCTGCATTGGACAGCCCGTCTTCTGTTCTGCACCCTCCCTCCTCTCTAAAAAGGAAAACAATTCGTGATTACTTTGGAGTACCTCCCTTAAACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0000151 GO:0000209 GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010564 GO:0010948 GO:0015630 GO:0016567 GO:0018130 GO:0019438 GO:0019538 GO:0019941 GO:0019985 GO:0023052 GO:0030163 GO:0031461 GO:0031464 GO:0031465 GO:0031570 GO:0031572 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0071897 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080008 GO:0090304 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901987 GO:1901988 GO:1902494 GO:1902749 GO:1902750 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

516

Amino Acids

57.08

Weight (kDa)

7.97

Isoelectric Point (pI)

50.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	59 - 187	4.1e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	60 - 188	2.7e-14	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	61 - 184	8.3e-17	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	61 - 186	1.2e-10	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	92 - 187	2.6e-08	WDR55
WD40_Gbeta	PF25391	96 - 189	6.1e-13	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	97 - 189	5.2e-14	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	102 - 176	1.4e-08	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	103 - 177	3.1e-07	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	104 - 190	4.6e-17	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	106 - 187	4.1e-11	WDHD1 first WD40 domain
Beta-prop_WDR75_1st	PF23869	116 - 281	6e-06	WD repeat-containing protein 75 first beta-propeller
Beta-prop_SCAP	PF24017	118 - 191	9.9e-06	SCAP Beta-propeller
WD40	PF00400	138 - 176	2.9e-06	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	213 - 350	3.5e-10	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	216 - 395	6.9e-07	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	219 - 395	1.2e-12	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	222 - 399	2.6e-07	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	238 - 397	1.2e-13	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	328 - 400	5.8e-11	THOC3 beta-propeller domain
WD40_Prp19	PF24814	330 - 399	3.5e-07	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	332 - 400	8.2e-06	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27640 AT3G27640
fragaria_vesca	FvH4_6g51280 FvH4_6g51280 FvH4_6g51280 FvH4_6g51280
malus_domestica	MD09G1027800.v1.1 MD17G1027700.v1.1 MD17G1028200.v1.1
prunus_persica	Prupe.3G290400_v2.0.a1 Prupe.3G291000_v2.0.a1
pyrus_communis	pycom111g02180
rosa_chinensis	RchiOBHm_Chr2g0172901
rosa_laevigata	RLG00000022140
rosa_multiflora	Rmu_co8216202.1_g000001 Rmu_sc0002979.1_g000002 Rmu_sc0013380.1_g000001 Rmu_sc0015618.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079450
rosa_rugosa	Rorug02G0563200 Rorug02G0563400
rosa_samantha	Rh2AG640100 Rh2AG640200 Rh2BG653600 Rh2BG653700 Rh2CG619200 Rh2DG667200
rosa_wichuraiana	Rw2G052840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	731
Acc16I	TGCGCA	1 cut(s)	309
Acc36I	ACCTGC	1 cut(s)	1030
AccB1I	GGYRCC	1 cut(s)	119
AccIII	TCCGGA	1 cut(s)	1301
AciI	CCGC	2 cut(s)	76, 252
AclI	AACGTT	1 cut(s)	935
AclWI	GGATC	2 cut(s)	1406, 1419
AcoI	YGGCCR	1 cut(s)	77
AcuI	CTGAAG	1 cut(s)	893
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	6 cut(s)	83, 218, 418, 906, 1378, 1530
AfiI	CCNNNNNNNGG	5 cut(s)	151, 1035, 1087, 1120, 1221
AflIII	ACRYGT	2 cut(s)	236, 625
AgsI	TTSAA	4 cut(s)	543, 760, 956, 1075
AhdI	GACNNNNNGTC	1 cut(s)	1465
AjiI	CACGTC	2 cut(s)	239, 672
Alw21I	GWGCWC	2 cut(s)	70, 138
Alw26I	GTCTC	2 cut(s)	496, 703
AlwI	GGATC	2 cut(s)	1406, 1419
AlwNI	CAGNNNCTG	1 cut(s)	371
Ama87I	CYCGRG	1 cut(s)	479
Aor13HI	TCCGGA	1 cut(s)	1301
AoxI	GGCC	3 cut(s)	77, 142, 1078
ApeKI	GCWGC	3 cut(s)	128, 992, 1451
AspLEI	GCGC	2 cut(s)	122, 310
AspS9I	GGNCC	2 cut(s)	143, 1110
AsuHPI	GGTGA	2 cut(s)	257, 386
AsuII	TTCGAA	1 cut(s)	1219
AvaI	CYCGRG	1 cut(s)	479
AvaII	GGWCC	1 cut(s)	1110
AxyI	CCTNAGG	1 cut(s)	1053
BaeGI	GKGCMC	1 cut(s)	438
BamHI	GGATCC	1 cut(s)	1411
BanI	GGYRCC	1 cut(s)	119
BanII	GRGCYC	2 cut(s)	70, 604
BarI	GAAGNNNNNNTAC	2 cut(s)	65, 97
BbsI	GAAGAC	3 cut(s)	1065, 1445, 1461
Bbv12I	GWGCWC	2 cut(s)	70, 138
BbvI	GCAGC	3 cut(s)	115, 979, 1438
BccI	CCATC	9 cut(s)	131, 145, 206, 424, 500, 1013, 1126, 1216, 1324
BceAI	ACGGC	3 cut(s)	64, 157, 572
BcgI	CGANNNNNNTGC	4 cut(s)	1007, 1041, 1345, 1379
BciVI	GTATCC	1 cut(s)	739
BclI	TGATCA	1 cut(s)	376
BcoDI	GTCTC	2 cut(s)	496, 703
BfaI	CTAG	5 cut(s)	45, 288, 831, 914, 1014
BfmI	CTRYAG	2 cut(s)	129, 1098
BfoI	RGCGCY	1 cut(s)	123
BfuAI	ACCTGC	1 cut(s)	1030
BfuI	GTATCC	1 cut(s)	739
BglII	AGATCT	3 cut(s)	6, 41, 844
BisI	GCNGC	4 cut(s)	77, 129, 993, 1452
BlsI	GCNGC	4 cut(s)	78, 130, 994, 1453
BmcAI	AGTACT	1 cut(s)	1378
Bme18I	GGWCC	1 cut(s)	1110
BmeRI	GACNNNNNGTC	1 cut(s)	1465
BmeT110I	CYCGRG	1 cut(s)	479
BmgBI	CACGTC	2 cut(s)	239, 672
BmgT120I	GGNCC	2 cut(s)	143, 1110
BmiI	GGNNCC	4 cut(s)	121, 511, 1112, 1413
BmrI	ACTGGG	1 cut(s)	1108
BmsI	GCATC	7 cut(s)	107, 263, 269, 319, 675, 935, 979
BmuI	ACTGGG	1 cut(s)	1108
BpiI	GAAGAC	3 cut(s)	1065, 1445, 1461
Bpu14I	TTCGAA	1 cut(s)	1219
BpuEI	CTTGAG	1 cut(s)	241
BsaHI	GRCGYC	1 cut(s)	120
BsaI	GGTCTC	1 cut(s)	703
BsaJI	CCNNGG	3 cut(s)	588, 614, 1431
BsaWI	WCCGGW	1 cut(s)	1301
BsaXI	ACNNNNNCTCC	4 cut(s)	1189, 1219, 1472, 1502
Bsc4I	CCNNNNNNNGG	5 cut(s)	151, 1035, 1087, 1120, 1221
Bse1I	ACTGG	2 cut(s)	946, 1114
Bse21I	CCTNAGG	1 cut(s)	1053
Bse3DI	GCAATG	1 cut(s)	702
BseAI	TCCGGA	1 cut(s)	1301
BseDI	CCNNGG	3 cut(s)	588, 614, 1431
BseGI	GGATG	4 cut(s)	217, 352, 466, 950
BseLI	CCNNNNNNNGG	5 cut(s)	151, 1035, 1087, 1120, 1221
BseMI	GCAATG	1 cut(s)	702
BseMII	CTCAG	4 cut(s)	792, 1018, 1067, 1190
BseNI	ACTGG	2 cut(s)	946, 1114
BseRI	GAGGAG	1 cut(s)	1480
BseSI	GKGCMC	1 cut(s)	438
BseX3I	CGGCCG	1 cut(s)	77
BseXI	GCAGC	3 cut(s)	115, 979, 1438
BsgI	GTGCAG	2 cut(s)	1266, 1463
Bsh1285I	CGRYCG	1 cut(s)	80
BshFI	GGCC	3 cut(s)	79, 144, 1080
BshNI	GGYRCC	1 cut(s)	119
BsiEI	CGRYCG	1 cut(s)	80
BsiHKAI	GWGCWC	2 cut(s)	70, 138
BsiHKCI	CYCGRG	1 cut(s)	479
BsiSI	CCGG	3 cut(s)	714, 949, 1302
BslFI	GGGAC	1 cut(s)	1096
BslI	CCNNNNNNNGG	5 cut(s)	151, 1035, 1087, 1120, 1221
BsmAI	GTCTC	2 cut(s)	496, 703
BsmFI	GGGAC	1 cut(s)	1096
BsnI	GGCC	3 cut(s)	79, 144, 1080
Bso31I	GGTCTC	1 cut(s)	703
BsoBI	CYCGRG	1 cut(s)	479
Bsp119I	TTCGAA	1 cut(s)	1219
Bsp1286I	GDGCHC	4 cut(s)	70, 138, 438, 604
Bsp13I	TCCGGA	1 cut(s)	1301
Bsp143I	GATC	5 cut(s)	6, 41, 376, 844, 1411
BspACI	CCGC	2 cut(s)	76, 252
BspANI	GGCC	3 cut(s)	79, 144, 1080
BspCNI	CTCAG	4 cut(s)	793, 1017, 1066, 1189
BspEI	TCCGGA	1 cut(s)	1301
BspLI	GGNNCC	4 cut(s)	121, 511, 1112, 1413
BspMAI	CTGCAG	2 cut(s)	133, 1102
BspMI	ACCTGC	1 cut(s)	1030
BspPI	GGATC	2 cut(s)	1406, 1419
BspT104I	TTCGAA	1 cut(s)	1219
BspT107I	GGYRCC	1 cut(s)	119
BspTNI	GGTCTC	1 cut(s)	703
BsrDI	GCAATG	1 cut(s)	702
BsrI	ACTGG	2 cut(s)	946, 1114
BssECI	CCNNGG	3 cut(s)	588, 614, 1431
BssMI	GATC	5 cut(s)	6, 41, 376, 844, 1411
BssNI	GRCGYC	1 cut(s)	120
BssT1I	CCWWGG	1 cut(s)	1431
Bst4CI	ACNGT	3 cut(s)	322, 824, 1159
BstACI	GRCGYC	1 cut(s)	120
BstBI	TTCGAA	1 cut(s)	1219
BstC8I	GCNNGC	1 cut(s)	185
BstDEI	CTNAG	4 cut(s)	801, 1004, 1053, 1176
BstDSI	CCRYGG	2 cut(s)	588, 614
BstF5I	GGATG	4 cut(s)	217, 352, 466, 950
BstH2I	RGCGCY	1 cut(s)	123
BstHHI	GCGC	2 cut(s)	122, 310
BstKTI	GATC	5 cut(s)	9, 44, 379, 847, 1414
BstMAI	GTCTC	2 cut(s)	496, 703
BstMBI	GATC	5 cut(s)	6, 41, 376, 844, 1411
BstMCI	CGRYCG	1 cut(s)	80
BstMWI	GCNNNNNNNGC	3 cut(s)	128, 565, 640
BstNSI	RCATGY	1 cut(s)	783
BstSFI	CTRYAG	2 cut(s)	129, 1098
BstSLI	GKGCMC	1 cut(s)	438
BstV1I	GCAGC	3 cut(s)	115, 979, 1438
BstV2I	GAAGAC	3 cut(s)	1065, 1445, 1461
BstX2I	RGATCY	4 cut(s)	6, 41, 844, 1411
BstYI	RGATCY	4 cut(s)	6, 41, 844, 1411
BstZI	CGGCCG	1 cut(s)	77
Bsu36I	CCTNAGG	1 cut(s)	1053
BsuI	GTATCC	1 cut(s)	739
BsuRI	GGCC	3 cut(s)	79, 144, 1080
BtgI	CCRYGG	2 cut(s)	588, 614
BtgZI	GCGATG	2 cut(s)	109, 1032
BtrI	CACGTC	2 cut(s)	239, 672
BtsCI	GGATG	4 cut(s)	217, 352, 466, 950
BtsI	GCAGTG	1 cut(s)	1107
BtsIMutI	CAGTG	4 cut(s)	318, 939, 1012, 1107
BveI	ACCTGC	1 cut(s)	1030
Cac8I	GCNNGC	1 cut(s)	185
CaiI	CAGNNNCTG	1 cut(s)	371
CfoI	GCGC	2 cut(s)	122, 310
Cfr13I	GGNCC	2 cut(s)	143, 1110
Csp6I	GTAC	6 cut(s)	82, 217, 417, 905, 1377, 1529
CspCI	CAANNNNNGTGG	2 cut(s)	570, 605
CviAII	CATG	6 cut(s)	199, 661, 780, 873, 877, 1231
CviQI	GTAC	6 cut(s)	82, 217, 417, 905, 1377, 1529
DdeI	CTNAG	4 cut(s)	801, 1004, 1053, 1176
DinI	GGCGCC	1 cut(s)	121
DpnI	GATC	5 cut(s)	8, 43, 378, 846, 1413
DpnII	GATC	5 cut(s)	6, 41, 376, 844, 1411
DrdI	GACNNNNNNGTC	1 cut(s)	731
DriI	GACNNNNNGTC	1 cut(s)	1465
DseDI	GACNNNNNNGTC	1 cut(s)	731
EaeI	YGGCCR	1 cut(s)	77
EagI	CGGCCG	1 cut(s)	77
Eam1105I	GACNNNNNGTC	1 cut(s)	1465
Ecl136II	GAGCTC	1 cut(s)	68
EclXI	CGGCCG	1 cut(s)	77
Eco130I	CCWWGG	1 cut(s)	1431
Eco24I	GRGCYC	2 cut(s)	70, 604
Eco31I	GGTCTC	1 cut(s)	703
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	1 cut(s)	1110
Eco52I	CGGCCG	1 cut(s)	77
Eco53kI	GAGCTC	1 cut(s)	68
Eco57I	CTGAAG	1 cut(s)	893
Eco81I	CCTNAGG	1 cut(s)	1053
Eco88I	CYCGRG	1 cut(s)	479
EcoICRI	GAGCTC	1 cut(s)	68
EcoRV	GATATC	1 cut(s)	331
EcoT14I	CCWWGG	1 cut(s)	1431
EcoT22I	ATGCAT	3 cut(s)	878, 1287, 1368
EcoT38I	GRGCYC	2 cut(s)	70, 604
EgeI	GGCGCC	1 cut(s)	121
EheI	GGCGCC	1 cut(s)	121
ErhI	CCWWGG	1 cut(s)	1431
FaeI	CATG	6 cut(s)	202, 664, 783, 876, 880, 1234
FaqI	GGGAC	1 cut(s)	1096
FatI	CATG	6 cut(s)	198, 660, 779, 872, 876, 1230
FbaI	TGATCA	1 cut(s)	376
Fnu4HI	GCNGC	4 cut(s)	77, 129, 993, 1452
FokI	GGATG	4 cut(s)	224, 359, 453, 957
FriOI	GRGCYC	2 cut(s)	70, 604
Fsp4HI	GCNGC	4 cut(s)	77, 129, 993, 1452
FspBI	CTAG	5 cut(s)	45, 288, 831, 914, 1014
FspI	TGCGCA	1 cut(s)	309
GlaI	GCGC	2 cut(s)	121, 309
GluI	GCNGC	4 cut(s)	77, 129, 993, 1452
HaeII	RGCGCY	1 cut(s)	123
HaeIII	GGCC	3 cut(s)	79, 144, 1080
HapII	CCGG	3 cut(s)	714, 949, 1302
HhaI	GCGC	2 cut(s)	122, 310
Hin1I	GRCGYC	1 cut(s)	120
Hin1II	CATG	6 cut(s)	202, 664, 783, 876, 880, 1234
Hin6I	GCGC	2 cut(s)	120, 308
HinP1I	GCGC	2 cut(s)	120, 308
HincII	GTYRAC	1 cut(s)	798
HindII	GTYRAC	1 cut(s)	798
HindIII	AAGCTT	2 cut(s)	641, 1259
HinfI	GANTC	6 cut(s)	60, 794, 1187, 1227, 1274, 1298
HpaII	CCGG	3 cut(s)	714, 949, 1302
HphI	GGTGA	2 cut(s)	257, 386
Hpy166II	GTNNAC	4 cut(s)	724, 798, 939, 1045
Hpy188III	TCNNGA	9 cut(s)	45, 404, 479, 501, 986, 1055, 1302, 1415, 1514
Hpy8I	GTNNAC	4 cut(s)	724, 798, 939, 1045
Hpy99I	CGWCG	3 cut(s)	209, 243, 1411
HpyAV	CCTTC	3 cut(s)	799, 1215, 1319
HpyCH4III	ACNGT	3 cut(s)	322, 824, 1159
HpyCH4IV	ACGT	5 cut(s)	238, 625, 671, 935, 1406
HpyF10VI	GCNNNNNNNGC	3 cut(s)	128, 565, 640
HpyF3I	CTNAG	4 cut(s)	801, 1004, 1053, 1176
HpySE526I	ACGT	5 cut(s)	238, 625, 671, 935, 1406
Hsp92I	GRCGYC	1 cut(s)	120
Hsp92II	CATG	6 cut(s)	202, 664, 783, 876, 880, 1234
HspAI	GCGC	2 cut(s)	120, 308
KasI	GGCGCC	1 cut(s)	119
Kpn2I	TCCGGA	1 cut(s)	1301
Ksp22I	TGATCA	1 cut(s)	376
Kzo9I	GATC	5 cut(s)	6, 41, 376, 844, 1411
LmnI	GCTCC	4 cut(s)	515, 551, 565, 716
Lsp1109I	GCAGC	3 cut(s)	115, 979, 1438
LweI	GCATC	7 cut(s)	107, 263, 269, 319, 675, 935, 979
MaeI	CTAG	5 cut(s)	45, 288, 831, 914, 1014
MaeII	ACGT	5 cut(s)	238, 625, 671, 935, 1406
MaeIII	GTNAC	4 cut(s)	592, 769, 1093, 1184
MalI	GATC	5 cut(s)	8, 43, 378, 846, 1413
MboI	GATC	5 cut(s)	6, 41, 376, 844, 1411
MboII	GAAGA	5 cut(s)	19, 1070, 1141, 1450, 1461
MflI	RGATCY	4 cut(s)	6, 41, 844, 1411
MhlI	GDGCHC	4 cut(s)	70, 138, 438, 604
MluCI	AATT	4 cut(s)	114, 357, 538, 1509
Mly113I	GGCGCC	1 cut(s)	120
MlyI	GAGTC	3 cut(s)	803, 1181, 1236
MmeI	TCCRAC	4 cut(s)	228, 317, 494, 1389
Mph1103I	ATGCAT	3 cut(s)	878, 1287, 1368
MroI	TCCGGA	1 cut(s)	1301
MseI	TTAA	4 cut(s)	342, 687, 969, 1538
MslI	CAYNNNNRTG	2 cut(s)	554, 778
MspI	CCGG	3 cut(s)	714, 949, 1302
MwoI	GCNNNNNNNGC	3 cut(s)	128, 565, 640
NarI	GGCGCC	1 cut(s)	120
NdeII	GATC	5 cut(s)	6, 41, 376, 844, 1411
NlaIII	CATG	6 cut(s)	202, 664, 783, 876, 880, 1234
NlaIV	GGNNCC	4 cut(s)	121, 511, 1112, 1413
NmuCI	GTSAC	2 cut(s)	769, 1184
NsbI	TGCGCA	1 cut(s)	309
NsiI	ATGCAT	3 cut(s)	878, 1287, 1368
NspI	RCATGY	1 cut(s)	783
NspV	TTCGAA	1 cut(s)	1219
PcsI	WCGNNNNNNNCGW	3 cut(s)	201, 622, 631
PfeI	GAWTC	3 cut(s)	60, 1274, 1298
PkrI	GCNGC	4 cut(s)	78, 130, 994, 1453
PleI	GAGTC	3 cut(s)	802, 1181, 1235
PluTI	GGCGCC	1 cut(s)	123
PpsI	GAGTC	3 cut(s)	802, 1181, 1235
Psp124BI	GAGCTC	1 cut(s)	70
Psp1406I	AACGTT	1 cut(s)	935
PspN4I	GGNNCC	4 cut(s)	121, 511, 1112, 1413
PspPI	GGNCC	2 cut(s)	143, 1110
PstI	CTGCAG	2 cut(s)	133, 1102
PstNI	CAGNNNCTG	1 cut(s)	371
PsuI	RGATCY	4 cut(s)	6, 41, 844, 1411
RsaI	GTAC	6 cut(s)	83, 218, 418, 906, 1378, 1530
RsaNI	GTAC	6 cut(s)	82, 217, 417, 905, 1377, 1529
RseI	CAYNNNNRTG	2 cut(s)	554, 778
SacI	GAGCTC	1 cut(s)	70
SaqAI	TTAA	4 cut(s)	342, 687, 969, 1538
SatI	GCNGC	4 cut(s)	77, 129, 993, 1452
Sau3AI	GATC	5 cut(s)	6, 41, 376, 844, 1411
Sau96I	GGNCC	2 cut(s)	143, 1110
ScaI	AGTACT	1 cut(s)	1378
SchI	GAGTC	3 cut(s)	803, 1181, 1236
SduI	GDGCHC	4 cut(s)	70, 138, 438, 604
SfaNI	GCATC	7 cut(s)	107, 263, 269, 319, 675, 935, 979
SfcI	CTRYAG	2 cut(s)	129, 1098
SfoI	GGCGCC	1 cut(s)	121
SfuI	TTCGAA	1 cut(s)	1219
SinI	GGWCC	1 cut(s)	1110
SmiMI	CAYNNNNRTG	2 cut(s)	554, 778
SmlI	CTYRAG	1 cut(s)	220
SmoI	CTYRAG	1 cut(s)	220
Sse9I	AATT	4 cut(s)	114, 357, 538, 1509
SsiI	CCGC	2 cut(s)	76, 252
SspDI	GGCGCC	1 cut(s)	119
SspMI	CTAG	5 cut(s)	45, 288, 831, 914, 1014
SstI	GAGCTC	1 cut(s)	70
StyI	CCWWGG	1 cut(s)	1431
TaaI	ACNGT	3 cut(s)	322, 824, 1159
TaiI	ACGT	5 cut(s)	241, 628, 674, 938, 1409
TaqI	TCGA	3 cut(s)	231, 500, 1219
TasI	AATT	4 cut(s)	114, 357, 538, 1509
TatI	WGTACW	3 cut(s)	416, 904, 1376
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	3 cut(s)	60, 1274, 1298
Tru1I	TTAA	4 cut(s)	342, 687, 969, 1538
Tru9I	TTAA	4 cut(s)	342, 687, 969, 1538
TscAI	CASTG	4 cut(s)	325, 946, 1012, 1107
TseFI	GTSAC	2 cut(s)	769, 1184
TseI	GCWGC	3 cut(s)	128, 992, 1451
Tsp45I	GTSAC	2 cut(s)	769, 1184
TspDTI	ATGAA	4 cut(s)	861, 1283, 1287, 1338
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	4 cut(s)	325, 946, 1012, 1107
VpaK11BI	GGWCC	1 cut(s)	1110
XbaI	TCTAGA	1 cut(s)	44
XceI	RCATGY	1 cut(s)	783
XcmI	CCANNNNNNNNNTGG	2 cut(s)	848, 1121
XspI	CTAG	5 cut(s)	45, 288, 831, 914, 1014
ZrmI	AGTACT	1 cut(s)	1378
Zsp2I	ATGCAT	3 cut(s)	878, 1287, 1368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.