Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005798

Physical Location & Seq

Genomic Coordinates Forward (+)

1998361 .. 2000605

Gene Structure 2245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1028200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGAGATGTAATTCAAGCTCCAAAAATATGCAGGGGTCAACTGCAGTGGTTACAGGGGCTCATATTGTTCCACGGGCAAAGCGTGTAAGGCGCGGAAAGGCTGTTTCCATGAGCATCACATCAGTTCTTTATCTTAAAGATGAGTTCTCCATTGCTACCGCCGGAGCTGTGGACAGCATTGTCAAGTTTTGGGATACCAGAAGTTTGAAAAATGTTGTCACACAGACATGCCCTCAACCGGAGTCAACTGAGAAGGAAAGACGATTACACGGTATAACTAGCTTATCCCAAGATTTAAATGGAGGTCCTGTTCAAGCGTTCACCGGATGCTGGATTGAAACATTTTTTGTTAAGTCAGTTATTAGCCCCGATGCTGCTCACATAGTCAGTGGCTCGAGCGATGGAAACGCCTATATTTGGCAGGTAAACAAGCCTCGGGAAGATCCCGTTATATTGAAAGGCCATGACAGGGAAGTTACAGCAGTAGATTGGTCCCAGTTTGAGGTCGGGAAGATGGCGACGTCGTCAGATGATTTTACGGTTCGCATTTGGAACTCTCAGAAAAGTTACTCCACAAGCACAAGCTCACCATCTACCGTTCGAAGAAGAGTTATGGCAATCCCAAGCGCAGAGTGTAGAAAGCTTCTGATGAGTGAATCAACGATGCATACCTCAAAGGTTTCCGGAAGTTTGTGTCCGTCAGATGAAGGATTAGACGAAACCAACTCACTAAATCCGGTTAGTACGCCCAGAATGAGTACTCCCCCGTCACAGAAGAAACAATCTATGTCGGATCCTGACTTTAGCAAGACCTTTGAGAAGACCCCCGAAGCTGCATCGGAGAGCCCGTCTTCTGTTCTGAACCCTCCCTCCTCCCTAAAAAGGAAAACAATCCGTGATTACTTTGGAGTACCTCCCTTAAACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0000151 GO:0000209 GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010564 GO:0010948 GO:0015630 GO:0016567 GO:0018130 GO:0019438 GO:0019538 GO:0019941 GO:0019985 GO:0023052 GO:0030163 GO:0031461 GO:0031464 GO:0031465 GO:0031570 GO:0031572 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0071897 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080008 GO:0090304 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901987 GO:1901988 GO:1902494 GO:1902749 GO:1902750 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

310

Amino Acids

33.81

Weight (kDa)

9.16

Isoelectric Point (pI)

57.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	121 - 191	7.1e-10	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	121 - 192	7.8e-08	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	121 - 193	5.2e-06	MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	121 - 197	5.1e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	122 - 196	1e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	122 - 197	7.3e-09	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	124 - 220	1.6e-07	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	126 - 191	2.7e-06	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27640 AT3G27640
fragaria_vesca	FvH4_6g51280 FvH4_6g51280 FvH4_6g51280 FvH4_6g51280
malus_domestica	MD09G1027800.v1.1 MD17G1027700.v1.1 MD17G1028200.v1.1
prunus_persica	Prupe.3G290400_v2.0.a1 Prupe.3G291000_v2.0.a1
pyrus_communis	pycom111g02180
rosa_chinensis	RchiOBHm_Chr2g0172901
rosa_laevigata	RLG00000022140
rosa_multiflora	Rmu_co8216202.1_g000001 Rmu_sc0002979.1_g000002 Rmu_sc0013380.1_g000001 Rmu_sc0015618.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079450
rosa_rugosa	Rorug02G0563200 Rorug02G0563400
rosa_samantha	Rh2AG640100 Rh2AG640200 Rh2BG653600 Rh2BG653700 Rh2CG619200 Rh2DG667200
rosa_wichuraiana	Rw2G052840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AatII	GACGTC	1 cut(s)	524
Acc36I	ACCTGC	1 cut(s)	412
AccII	CGCG	1 cut(s)	93
AccIII	TCCGGA	1 cut(s)	683
AciI	CCGC	2 cut(s)	93, 159
AclWI	GGATC	3 cut(s)	437, 788, 801
AcyI	GRCGYC	1 cut(s)	521
AfaI	GTAC	3 cut(s)	745, 760, 912
AfiI	CCNNNNNNNGG	6 cut(s)	238, 330, 417, 469, 502, 882
AgsI	TTSAA	5 cut(s)	15, 208, 314, 338, 457
AloI	GAACNNNNNNTCC	4 cut(s)	294, 326, 854, 886
AluBI	AGCT	6 cut(s)	18, 167, 282, 585, 643, 833
AluI	AGCT	6 cut(s)	18, 167, 282, 585, 643, 833
AlwI	GGATC	3 cut(s)	437, 788, 801
Ama87I	CYCGRG	2 cut(s)	394, 435
Aor13HI	TCCGGA	1 cut(s)	683
AoxI	GGCC	1 cut(s)	460
ApeKI	GCWGC	2 cut(s)	374, 833
AspLEI	GCGC	2 cut(s)	93, 629
AspS9I	GGNCC	2 cut(s)	305, 492
AsuHPI	GGTGA	2 cut(s)	313, 579
AsuII	TTCGAA	1 cut(s)	601
AvaI	CYCGRG	2 cut(s)	394, 435
AvaII	GGWCC	2 cut(s)	305, 492
BamHI	GGATCC	1 cut(s)	793
BanII	GRGCYC	2 cut(s)	61, 848
BbsI	GAAGAC	2 cut(s)	827, 843
BbvI	GCAGC	2 cut(s)	361, 820
BccI	CCATC	3 cut(s)	395, 508, 598
BciVI	GTATCC	1 cut(s)	187
BfaI	CTAG	1 cut(s)	279
BfmI	CTRYAG	1 cut(s)	42
BfuAI	ACCTGC	1 cut(s)	412
BfuI	GTATCC	1 cut(s)	187
BisI	GCNGC	2 cut(s)	375, 834
BlsI	GCNGC	2 cut(s)	376, 835
BmcAI	AGTACT	1 cut(s)	760
Bme18I	GGWCC	2 cut(s)	305, 492
BmeT110I	CYCGRG	2 cut(s)	394, 435
BmgT120I	GGNCC	2 cut(s)	305, 492
BmiI	GGNNCC	2 cut(s)	494, 795
BmrI	ACTGGG	1 cut(s)	490
BmsI	GCATC	5 cut(s)	123, 317, 361, 654, 845
BmuI	ACTGGG	1 cut(s)	490
BpiI	GAAGAC	2 cut(s)	827, 843
Bpu14I	TTCGAA	1 cut(s)	601
BsaHI	GRCGYC	1 cut(s)	521
BsaJI	CCNNGG	2 cut(s)	71, 434
BsaWI	WCCGGW	4 cut(s)	238, 323, 683, 736
BsaXI	ACNNNNNCTCC	6 cut(s)	294, 324, 833, 854, 863, 884
Bsc4I	CCNNNNNNNGG	6 cut(s)	238, 330, 417, 469, 502, 882
Bse1I	ACTGG	1 cut(s)	496
Bse3DI	GCAATG	1 cut(s)	150
BseAI	TCCGGA	1 cut(s)	683
BseDI	CCNNGG	2 cut(s)	71, 434
BseGI	GGATG	1 cut(s)	332
BseLI	CCNNNNNNNGG	6 cut(s)	238, 330, 417, 469, 502, 882
BseMI	GCAATG	1 cut(s)	150
BseMII	CTCAG	2 cut(s)	240, 572
BseNI	ACTGG	1 cut(s)	496
BseRI	GAGGAG	1 cut(s)	862
BseXI	GCAGC	2 cut(s)	361, 820
Bsh1236I	CGCG	1 cut(s)	93
BshFI	GGCC	1 cut(s)	462
BsiHKCI	CYCGRG	2 cut(s)	394, 435
BsiSI	CCGG	5 cut(s)	162, 239, 324, 684, 737
BslFI	GGGAC	1 cut(s)	478
BslI	CCNNNNNNNGG	6 cut(s)	238, 330, 417, 469, 502, 882
BsmFI	GGGAC	1 cut(s)	478
BsnI	GGCC	1 cut(s)	462
BsoBI	CYCGRG	2 cut(s)	394, 435
Bsp119I	TTCGAA	1 cut(s)	601
Bsp1286I	GDGCHC	2 cut(s)	61, 848
Bsp13I	TCCGGA	1 cut(s)	683
Bsp143I	GATC	2 cut(s)	442, 793
BspACI	CCGC	2 cut(s)	93, 159
BspANI	GGCC	1 cut(s)	462
BspCNI	CTCAG	2 cut(s)	241, 571
BspEI	TCCGGA	1 cut(s)	683
BspFNI	CGCG	1 cut(s)	93
BspLI	GGNNCC	2 cut(s)	494, 795
BspMAI	CTGCAG	1 cut(s)	46
BspMI	ACCTGC	1 cut(s)	412
BspPI	GGATC	3 cut(s)	437, 788, 801
BspT104I	TTCGAA	1 cut(s)	601
BsrDI	GCAATG	1 cut(s)	150
BsrI	ACTGG	1 cut(s)	496
BssECI	CCNNGG	2 cut(s)	71, 434
BssMI	GATC	2 cut(s)	442, 793
BssNI	GRCGYC	1 cut(s)	521
Bst4CI	ACNGT	3 cut(s)	272, 541, 598
Bst6I	CTCTTC	1 cut(s)	601
BstACI	GRCGYC	1 cut(s)	521
BstBI	TTCGAA	1 cut(s)	601
BstDEI	CTNAG	2 cut(s)	249, 558
BstDSI	CCRYGG	1 cut(s)	71
BstF5I	GGATG	1 cut(s)	332
BstFNI	CGCG	1 cut(s)	93
BstHHI	GCGC	2 cut(s)	93, 629
BstKTI	GATC	2 cut(s)	445, 796
BstMBI	GATC	2 cut(s)	442, 793
BstMWI	GCNNNNNNNGC	1 cut(s)	88
BstNSI	RCATGY	1 cut(s)	231
BstSFI	CTRYAG	1 cut(s)	42
BstUI	CGCG	1 cut(s)	93
BstV1I	GCAGC	2 cut(s)	361, 820
BstV2I	GAAGAC	2 cut(s)	827, 843
BstX2I	RGATCY	2 cut(s)	442, 793
BstYI	RGATCY	2 cut(s)	442, 793
BsuI	GTATCC	1 cut(s)	187
BsuRI	GGCC	1 cut(s)	462
BtgI	CCRYGG	1 cut(s)	71
BtgZI	GCGATG	1 cut(s)	414
BtsCI	GGATG	1 cut(s)	332
BtsI	GCAGTG	1 cut(s)	51
BtsIMutI	CAGTG	2 cut(s)	51, 394
BveI	ACCTGC	1 cut(s)	412
CfoI	GCGC	2 cut(s)	93, 629
Cfr13I	GGNCC	2 cut(s)	305, 492
Csp6I	GTAC	3 cut(s)	744, 759, 911
CspCI	CAANNNNNGTGG	2 cut(s)	27, 62
CviAII	CATG	3 cut(s)	109, 228, 464
CviQI	GTAC	3 cut(s)	744, 759, 911
DdeI	CTNAG	2 cut(s)	249, 558
DpnI	GATC	2 cut(s)	444, 795
DpnII	GATC	2 cut(s)	442, 793
DraI	TTTAAA	1 cut(s)	297
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
Eam1104I	CTCTTC	1 cut(s)	601
EarI	CTCTTC	1 cut(s)	601
Eco24I	GRGCYC	2 cut(s)	61, 848
Eco47I	GGWCC	2 cut(s)	305, 492
Eco88I	CYCGRG	2 cut(s)	394, 435
EcoO109I	RGGNCCY	1 cut(s)	305
EcoT22I	ATGCAT	1 cut(s)	669
EcoT38I	GRGCYC	2 cut(s)	61, 848
FaeI	CATG	3 cut(s)	112, 231, 467
FaqI	GGGAC	1 cut(s)	478
FatI	CATG	3 cut(s)	108, 227, 463
Fnu4HI	GCNGC	2 cut(s)	375, 834
FokI	GGATG	1 cut(s)	339
FriOI	GRGCYC	2 cut(s)	61, 848
Fsp4HI	GCNGC	2 cut(s)	375, 834
FspBI	CTAG	1 cut(s)	279
GlaI	GCGC	2 cut(s)	92, 628
GluI	GCNGC	2 cut(s)	375, 834
HaeIII	GGCC	1 cut(s)	462
HapII	CCGG	5 cut(s)	162, 239, 324, 684, 737
HhaI	GCGC	2 cut(s)	93, 629
Hin1I	GRCGYC	1 cut(s)	521
Hin1II	CATG	3 cut(s)	112, 231, 467
Hin6I	GCGC	2 cut(s)	91, 627
HinP1I	GCGC	2 cut(s)	91, 627
HincII	GTYRAC	2 cut(s)	39, 246
HindII	GTYRAC	2 cut(s)	39, 246
HindIII	AAGCTT	1 cut(s)	641
HinfI	GANTC	2 cut(s)	242, 656
HpaII	CCGG	5 cut(s)	162, 239, 324, 684, 737
HphI	GGTGA	2 cut(s)	313, 579
Hpy166II	GTNNAC	5 cut(s)	39, 172, 246, 321, 427
Hpy188I	TCNGA	7 cut(s)	529, 561, 648, 703, 793, 841, 861
Hpy188III	TCNNGA	4 cut(s)	437, 508, 684, 797
Hpy8I	GTNNAC	5 cut(s)	39, 172, 246, 321, 427
Hpy99I	CGWCG	2 cut(s)	523, 526
HpyAV	CCTTC	2 cut(s)	247, 701
HpyCH4III	ACNGT	3 cut(s)	272, 541, 598
HpyCH4IV	ACGT	1 cut(s)	521
HpyCH4V	TGCA	4 cut(s)	31, 44, 667, 836
HpyF10VI	GCNNNNNNNGC	1 cut(s)	88
HpyF3I	CTNAG	2 cut(s)	249, 558
HpySE526I	ACGT	1 cut(s)	521
Hsp92I	GRCGYC	1 cut(s)	521
Hsp92II	CATG	3 cut(s)	112, 231, 467
HspAI	GCGC	2 cut(s)	91, 627
Kpn2I	TCCGGA	1 cut(s)	683
Kzo9I	GATC	2 cut(s)	442, 793
LmnI	GCTCC	2 cut(s)	23, 164
Lsp1109I	GCAGC	2 cut(s)	361, 820
LweI	GCATC	5 cut(s)	123, 317, 361, 654, 845
MaeI	CTAG	1 cut(s)	279
MaeII	ACGT	1 cut(s)	521
MaeIII	GTNAC	5 cut(s)	49, 217, 475, 566, 768
MalI	GATC	2 cut(s)	444, 795
MboI	GATC	2 cut(s)	442, 793
MboII	GAAGA	7 cut(s)	452, 523, 615, 618, 787, 832, 843
MflI	RGATCY	2 cut(s)	442, 793
MhlI	GDGCHC	2 cut(s)	61, 848
MluCI	AATT	1 cut(s)	10
MlyI	GAGTC	1 cut(s)	251
MmeI	TCCRAC	1 cut(s)	771
MnlI	CCTC	9 cut(s)	243, 296, 444, 496, 682, 876, 880, 883, 924
Mph1103I	ATGCAT	1 cut(s)	669
MroI	TCCGGA	1 cut(s)	683
MseI	TTAA	4 cut(s)	135, 296, 351, 920
MslI	CAYNNNNRTG	1 cut(s)	226
MspI	CCGG	5 cut(s)	162, 239, 324, 684, 737
MvnI	CGCG	1 cut(s)	93
MwoI	GCNNNNNNNGC	1 cut(s)	88
NdeII	GATC	2 cut(s)	442, 793
NlaIII	CATG	3 cut(s)	112, 231, 467
NlaIV	GGNNCC	2 cut(s)	494, 795
NmuCI	GTSAC	2 cut(s)	217, 768
NsiI	ATGCAT	1 cut(s)	669
NspI	RCATGY	1 cut(s)	231
NspV	TTCGAA	1 cut(s)	601
PaeR7I	CTCGAG	1 cut(s)	394
PcsI	WCGNNNNNNNCGW	2 cut(s)	79, 845
PfeI	GAWTC	1 cut(s)	656
PflFI	GACNNNGTC	1 cut(s)	523
PkrI	GCNGC	2 cut(s)	376, 835
PleI	GAGTC	1 cut(s)	250
PpsI	GAGTC	1 cut(s)	250
PpuMI	RGGWCCY	1 cut(s)	305
Psp5II	RGGWCCY	1 cut(s)	305
PspN4I	GGNNCC	2 cut(s)	494, 795
PspPI	GGNCC	2 cut(s)	305, 492
PspPPI	RGGWCCY	1 cut(s)	305
PspXI	VCTCGAGB	1 cut(s)	394
PstI	CTGCAG	1 cut(s)	46
PsuI	RGATCY	2 cut(s)	442, 793
PsyI	GACNNNGTC	1 cut(s)	523
RsaI	GTAC	3 cut(s)	745, 760, 912
RsaNI	GTAC	3 cut(s)	744, 759, 911
RseI	CAYNNNNRTG	1 cut(s)	226
SaqAI	TTAA	4 cut(s)	135, 296, 351, 920
SatI	GCNGC	2 cut(s)	375, 834
Sau3AI	GATC	2 cut(s)	442, 793
Sau96I	GGNCC	2 cut(s)	305, 492
ScaI	AGTACT	1 cut(s)	760
SchI	GAGTC	1 cut(s)	251
SduI	GDGCHC	2 cut(s)	61, 848
SfaNI	GCATC	5 cut(s)	123, 317, 361, 654, 845
SfcI	CTRYAG	1 cut(s)	42
Sfr274I	CTCGAG	1 cut(s)	394
SfuI	TTCGAA	1 cut(s)	601
SinI	GGWCC	2 cut(s)	305, 492
SlaI	CTCGAG	1 cut(s)	394
SmiI	ATTTAAAT	1 cut(s)	297
SmiMI	CAYNNNNRTG	1 cut(s)	226
SmlI	CTYRAG	1 cut(s)	394
SmoI	CTYRAG	1 cut(s)	394
Sse9I	AATT	1 cut(s)	10
SsiI	CCGC	2 cut(s)	93, 159
SspMI	CTAG	1 cut(s)	279
SwaI	ATTTAAAT	1 cut(s)	297
TaaI	ACNGT	3 cut(s)	272, 541, 598
TaiI	ACGT	1 cut(s)	524
TaqI	TCGA	2 cut(s)	395, 601
TasI	AATT	1 cut(s)	10
TatI	WGTACW	1 cut(s)	758
TfiI	GAWTC	1 cut(s)	656
Tru1I	TTAA	4 cut(s)	135, 296, 351, 920
Tru9I	TTAA	4 cut(s)	135, 296, 351, 920
TscAI	CASTG	2 cut(s)	51, 394
TseFI	GTSAC	2 cut(s)	217, 768
TseI	GCWGC	2 cut(s)	374, 833
Tsp45I	GTSAC	2 cut(s)	217, 768
TspDTI	ATGAA	1 cut(s)	720
TspGWI	ACGGA	2 cut(s)	687, 884
TspRI	CASTG	2 cut(s)	51, 394
Tth111I	GACNNNGTC	1 cut(s)	523
VpaK11BI	GGWCC	2 cut(s)	305, 492
XceI	RCATGY	1 cut(s)	231
XcmI	CCANNNNNNNNNTGG	1 cut(s)	296
XhoI	CTCGAG	1 cut(s)	394
XspI	CTAG	1 cut(s)	279
ZraI	GACGTC	1 cut(s)	522
ZrmI	AGTACT	1 cut(s)	760
Zsp2I	ATGCAT	1 cut(s)	669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.