MD17G1151100.v1.1

CCAAT enhancer-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008077
Physical Location & Seq
Reverse (-)
13925006 .. 13933006
8001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1151100.v1.1.491

Sequence Viewer

Length: 3075 bp
ATGGCGGATTCAAAATCAAAGAAATCCGCAAGCAACCCAGAAGACATAGAGCACCTCCGGTCCGACGTAGCCTCCTTCGCTTCCTCTCTCGGCCTCGCCTCCTCTCTCCCTTCCTCCGGCTTCAATGATGTCGATTTTCGCAATCCCGGTCCCAAAAAACCCCACAAGAAGTCCAAACCAGCTCCAATCCAGAACCCCACCAAAACCCAGAAGCCAAACAAGCCAAATTTCAAGCCCAATGAAAACCAGAAGCCGAAACTCACACTTTCTTCACTGGAAGAAAACAGCAACAAGGACAAAGCGAGGAACTTTGAGAAGTTCAAGAACCTCCCGAAGCTGCCATTGATGTCGGCAAACAACTTGGGAGTGTGGTATGAGGAAGCTGAGGAGTTGGAGGGTAAAGTGCTTGCGAGTGGGAAGAAAGTGGAGGTGAAGAATGCGGAGGAGTGGAATATTGTGGTGGCAAAGAAGAGAGAGCTTGGAGAAAGGTTAATGGCGCAGTACGTAGCGGACTACGAGTCTTCAAAGGGGAAGAGTGGGGATATCAAGCTGTTGCTTACTACGCAGAGGTCAGGGACTGCTTCTGATAAGATATCGGCGTTTTCGGTGTTGGTTGGGGATAATCCCATTGCCAATATGAGGTCCCTTGATGCACTTATAGGAATGGTGACCTCAAAAGTGGGAAAGCGATATGCATTTGCAGGGTTTGAAGCATTGAGAGAGTTGTTTCTTACAAGTTTGTTACCTGATCGTAAGCTTAAGAACCTCTTACAACGGCCATTAAATCATGTTCCTGAGACAAAAGATGGTTATTCTCTTCTGCTTCTATGGTATTGGGAGGAGTGCTTGAAACAAAGGTATGAACGCTATGTTTTTGCTCTTGAGGAAGCATCAAAAGATATGTTACCTGAACTAAAAAACAAGGCATTGAAGACTATATATGTGCTGCTAAAGAATAAATCGGAGCAGGAGCGCAGATTACTGTCTGCACTAGTTAACAAACTGGGAGATCCTAAAAATAAGGGCGCTTCTGATGCTGATTTTCACTTATCAAACCTTTTGTCTGATCATCCAAACATGAAGGCAGTGGTGATTGATGAGGTGGACTCTTTTCTCTTTCGCCCACGTTTGACTCCACAAGCAAAATATCATGCAGTTAACTTTTTGAGCCAAATGCGTCTAACTCATAAAGGAGATGGACCAAAGGTGGCAAAACGTTTAATCGATGTTTACTTTTCACTTTTCAAGGTACTTATTAATGAAGCTAATGCTGGTGAAAAGATGGATAAAAAAGGGAAAGCATGGTCTAAAAAACCACTCAGTTCTAATGAGGATAAGACTTCATCGGATTCTCATGTTGAATTGGATTCACGCCTTCTATCAGCTCTACTGGTGGGAGTCAATAGAGCATTTCCCTTTGTCTCAAGCAATGAGGCCGATGACATTGTTGAGGTCCAAACACCAATGCTTTTCCAGCTGGTTCATTCTAAGAATTTTAACGTAGGAGTTCAAGCATTAATGCTTCTCGATAAGATCTCGTCTAAGAATCAAATCATCAGTGATAGATTTTACAGGGCCTTGTACTCAAAACTACTACTTCCAGCTGCCATGAATACTTCTAAGGCTGAAATGTTTATTGGACTTATTCTGAGGGCAATGAAAAATGATGTGAACTTGAAGCGTGCGGCTGCGTTCGCAAAGCGTGTATTGCAGGTTGCGCTTCAGCAGCCACCTCAATATGCATGTGGATGTCTCTTCCTTCTCTCTGAAGTTCTTAAAGCAAGACCTCTGTTATGGAACATGGTGCTTCAGAATGAGTCAGTTGATGATGAACTTGAGCATTTTGAAGATGTTCGAGAAGAAACTGATGATAAACCCACCCCTGTGCCAGAGAAACAAGAACTTGATGTTGAGCTTATGCACAGCAATGATGCTGTCAATTCTGACCATGATTCTTTAGAAGATGATGAGGAGTCCGCTGCCTCTTATTCTGATGATGAAGGTTCTGATGAAGCAGAGGAGTTTCTTGTGACAAAAGGTCAAAATGATCCCAAACCAGCCCTTGCTGGTGGGCAACAACCACAAGCATCATCTGAGAGCCCTCGCTTGCCTGGAGGCTATGATCCACGACACAGAGAGCCCTCTTATTGTAATGCGGATCGTGTGAGCTGGTGGGAGCTGACAGTACTTTCTTCACATGTGCACCCTTCAGTTTCTACCATGGCAAAGACTCTACTCTCTGGGGCGAACATTGTCTACAATGGAAACCCGCTGAATGACCTATCACTTACTGCTTTTCTGGACAAGTTTATGGAGAAGAAACCAAAGCAAAGCGCATGGCATGGCGGTTCACAAATTGAACCTGCTAAAAAGCTTGACATGGCCAACCAATTAATCGGACCCGAGATTCTCTCATTGGCCGAGGAAGATGTAGCCCCGGAAGATCTTGTCTTCCACAAGTTCTATATGAACAAAATGAATTCTTCAAAGAAACCAAAGAAGAAAAAGAAGAAATCAACTGAAGATGAAGGCGCTGCAGATTTGTTTGACGTGGATGGGGGTGGCGGTGATGATAGTGATAACGAAGAGATTGACAATATGCTGGATTCTGCTGGTGTTTCTATCGAAGCAGACGGTGACTATGATTATGATGATCTGGACCAAGTTGCCAATGAAGATGATGAAGACTTGGTTGCTAATGTCAGTGATACTGAGCTGGACCTTCCCTCGGACAGTGGGGAGGGAGAAGACTTTGATGCCATTGCGGACGATGGCCTGAGTGATGATGATGATGATGCTATTGATATAGGAGATGCAGACGATGGTGACATTGACATAGGTGATGCAGACGATGATGATGACATTGACATAGGAGATGCAGATGACAGCTTTGAAGAGGATGAGGACGAGAAGAATGTTAAAAGCAAAAGTAAACGGAAGCGAGGGAAGTCTGGAGCGTCGCCGTTTGCAAGTCTTGAAGAATTTGAGCATCTGTTGAATGATAATGTTCCTGCTGAAAAGGAATCTAGAAAGAAGAAGCCAAAGTCACGAAAGAAGAGAAAGTCATTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

1025

Amino Acids

113.95

Weight (kDa)

4.98

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 503 - 742 5e-44 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1702, 2371
AccI GTMKAC 1 cut(s) 2256
AclI AACGTT 1 cut(s) 1216
AclWI GGATC 4 cut(s) 1004, 2042, 2117, 2166
AcoI YGGCCR 3 cut(s) 776, 2382, 2418
AcsI RAATTY 4 cut(s) 226, 1492, 2479, 2981
AcuI CTGAAG 5 cut(s) 1706, 1788, 1793, 2193, 2541
AfaI GTAC 4 cut(s) 503, 1251, 1583, 2187
AfiI CCNNNNNNNGG 3 cut(s) 116, 639, 2728
AflII CTTAAG 1 cut(s) 758
AflIII ACRYGT 1 cut(s) 2197
AhdI GACNNNNNGTC 2 cut(s) 517, 2037
AhlI ACTAGT 1 cut(s) 991
AjiI CACGTC 1 cut(s) 2551
AjnI CCWGG 1 cut(s) 2110
AjuI GAANNNNNNNTTGG 2 cut(s) 1620, 1652
AleI CACNNNNGTG 1 cut(s) 1883
Alw21I GWGCWC 2 cut(s) 54, 2205
Alw26I GTCTC 3 cut(s) 791, 1426, 1757
Alw44I GTGCAC 1 cut(s) 2201
AlwI GGATC 4 cut(s) 1004, 2042, 2117, 2166
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 2402
AoxI GGCC 7 cut(s) 91, 776, 1434, 1575, 2382, 2418, 2773
ApaLI GTGCAC 1 cut(s) 2201
ApeKI GCWGC 7 cut(s) 337, 946, 1604, 1688, 1726, 1979, 2534
ApoI RAATTY 4 cut(s) 226, 1492, 2479, 2981
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
AseI ATTAAT 3 cut(s) 1257, 1517, 2393
Asp700I GAANNNNTTC 1 cut(s) 1851
AspLEI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2534
AspS9I GGNCC 9 cut(s) 60, 149, 642, 1199, 1453, 1575, 2399, 2659, 2719
AsuC2I CCSGG 2 cut(s) 147, 2438
AsuHPI GGTGA 8 cut(s) 442, 679, 1102, 1286, 2579, 2648, 2837, 2852
AvaI CYCGRG 1 cut(s) 2402
AvaII GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2659, 2719
BaeGI GKGCMC 1 cut(s) 2205
BalI TGGCCA 1 cut(s) 2384
BanII GRGCYC 2 cut(s) 2102, 2142
BbsI GAAGAC 6 cut(s) 48, 513, 938, 2443, 2691, 2754
Bbv12I GWGCWC 2 cut(s) 54, 2205
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 7 cut(s) 324, 933, 1591, 1675, 1738, 1966, 2521
BccI CCATC 6 cut(s) 800, 1190, 1276, 2549, 2765, 2816
BceAI ACGGC 2 cut(s) 791, 2947
BciT130I CCWGG 1 cut(s) 2112
BclI TGATCA 1 cut(s) 1066
BcnI CCSGG 2 cut(s) 147, 2438
BcoDI GTCTC 3 cut(s) 791, 1426, 1757
BcuI ACTAGT 1 cut(s) 991
BfaI CTAG 2 cut(s) 992, 3027
BfmI CTRYAG 1 cut(s) 2535
BfoI RGCGCY 2 cut(s) 1029, 2535
BfrI CTTAAG 1 cut(s) 758
BfuAI ACCTGC 2 cut(s) 1702, 2371
BglII AGATCT 2 cut(s) 1533, 2443
BisI GCNGC 8 cut(s) 338, 947, 1605, 1686, 1689, 1727, 1980, 2535
BlsI GCNGC 8 cut(s) 339, 948, 1606, 1687, 1690, 1728, 1981, 2536
BmcAI AGTACT 1 cut(s) 2187
Bme1390I CCNGG 3 cut(s) 147, 2112, 2438
Bme18I GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2659, 2719
BmeRI GACNNNNNGTC 2 cut(s) 517, 2037
BmeT110I CYCGRG 1 cut(s) 2402
BmgBI CACGTC 1 cut(s) 2551
BmgT120I GGNCC 9 cut(s) 60, 149, 642, 1199, 1453, 1575, 2399, 2659, 2719
BmiI GGNNCC 3 cut(s) 151, 644, 2401
BmrFI CCNGG 3 cut(s) 147, 2112, 2438
BmrI ACTGGG 1 cut(s) 1013
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 6 cut(s) 48, 513, 938, 2443, 2691, 2754
BplI GAGNNNNNCTC 4 cut(s) 1091, 1123, 2396, 2428
BpmI CTGGAG 2 cut(s) 2133, 2973
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 3 cut(s) 902, 1408, 1856
BpuMI CCSGG 2 cut(s) 147, 2438
Bsa29I ATCGAT 1 cut(s) 1224
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 4 cut(s) 2220, 2421, 2436, 2727
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 639, 2728
Bse1I ACTGG 3 cut(s) 279, 1008, 1395
Bse3DI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2760
BseBI CCWGG 1 cut(s) 2112
BseCI ATCGAT 1 cut(s) 1224
BseDI CCNNGG 4 cut(s) 2220, 2421, 2436, 2727
BseGI GGATG 4 cut(s) 1069, 1754, 2560, 2905
BseLI CCNNNNNNNGG 3 cut(s) 116, 639, 2728
BseMI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2760
BseMII CTCAG 7 cut(s) 375, 786, 1333, 1640, 2085, 2703, 2768
BseNI ACTGG 3 cut(s) 279, 1008, 1395
BseRI GAGGAG 6 cut(s) 91, 401, 458, 854, 1985, 2033
BseSI GKGCMC 1 cut(s) 2205
BseXI GCAGC 7 cut(s) 324, 933, 1591, 1675, 1738, 1966, 2521
BsgI GTGCAG 1 cut(s) 972
BshFI GGCC 7 cut(s) 93, 778, 1436, 1577, 2384, 2420, 2775
BshVI ATCGAT 1 cut(s) 1224
BsiHKAI GWGCWC 2 cut(s) 54, 2205
BsiHKCI CYCGRG 1 cut(s) 2402
BsiSI CCGG 4 cut(s) 58, 117, 147, 2438
BslFI GGGAC 3 cut(s) 135, 589, 628
BslI CCNNNNNNNGG 3 cut(s) 116, 639, 2728
BsmAI GTCTC 3 cut(s) 791, 1426, 1757
BsmFI GGGAC 3 cut(s) 135, 589, 628
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 7 cut(s) 93, 778, 1436, 1577, 2384, 2420, 2775
BsoBI CYCGRG 1 cut(s) 2402
Bsp1286I GDGCHC 4 cut(s) 54, 2102, 2142, 2205
Bsp143I GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
Bsp19I CCATGG 1 cut(s) 2220
BspANI GGCC 7 cut(s) 93, 778, 1436, 1577, 2384, 2420, 2775
BspCNI CTCAG 7 cut(s) 376, 787, 1332, 1641, 2086, 2704, 2769
BspDI ATCGAT 1 cut(s) 1224
BspLI GGNNCC 3 cut(s) 151, 644, 2401
BspMAI CTGCAG 1 cut(s) 2539
BspMI ACCTGC 2 cut(s) 1702, 2371
BspPI GGATC 4 cut(s) 1004, 2042, 2117, 2166
BspTI CTTAAG 1 cut(s) 758
BsrDI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2760
BsrI ACTGG 3 cut(s) 279, 1008, 1395
BssECI CCNNGG 4 cut(s) 2220, 2421, 2436, 2727
BssMI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
BssT1I CCWWGG 1 cut(s) 2220
Bst2UI CCWGG 1 cut(s) 2112
Bst4CI ACNGT 4 cut(s) 984, 2185, 2636, 2735
Bst6I CTCTTC 7 cut(s) 464, 527, 822, 1760, 2580, 2889, 3050
BstAFI CTTAAG 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 505
BstC8I GCNNGC 4 cut(s) 31, 408, 1683, 2108
BstDSI CCRYGG 1 cut(s) 2220
BstEII GGTNACC 1 cut(s) 667
BstF5I GGATG 4 cut(s) 1069, 1754, 2560, 2905
BstH2I RGCGCY 2 cut(s) 1029, 2535
BstHHI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2534
BstKTI GATC 9 cut(s) 751, 1012, 1069, 1536, 2050, 2125, 2161, 2446, 2656
BstMAI GTCTC 3 cut(s) 791, 1426, 1757
BstMBI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
BstMWI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
BstNI CCWGG 1 cut(s) 2112
BstNSI RCATGY 2 cut(s) 1746, 2201
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 3 cut(s) 145, 2110, 2436
BstSFI CTRYAG 1 cut(s) 2535
BstSLI GKGCMC 1 cut(s) 2205
BstSNI TACGTA 1 cut(s) 505
BstV1I GCAGC 7 cut(s) 324, 933, 1591, 1675, 1738, 1966, 2521
BstV2I GAAGAC 6 cut(s) 48, 513, 938, 2443, 2691, 2754
BstX2I RGATCY 3 cut(s) 1009, 1533, 2443
BstYI RGATCY 3 cut(s) 1009, 1533, 2443
Bsu15I ATCGAT 1 cut(s) 1224
BsuRI GGCC 7 cut(s) 93, 778, 1436, 1577, 2384, 2420, 2775
BsuTUI ATCGAT 1 cut(s) 1224
BtgI CCRYGG 1 cut(s) 2220
BtrI CACGTC 1 cut(s) 2551
BtsCI GGATG 4 cut(s) 1069, 1754, 2560, 2905
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 5 cut(s) 272, 1092, 1564, 2710, 2740
BveI ACCTGC 2 cut(s) 1702, 2371
Cac8I GCNNGC 4 cut(s) 31, 408, 1683, 2108
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2534
Cfr13I GGNCC 9 cut(s) 60, 149, 642, 1199, 1453, 1575, 2399, 2659, 2719
ClaI ATCGAT 1 cut(s) 1224
CpoI CGGWCCG 1 cut(s) 60
CseI GACGC 2 cut(s) 1166, 2946
Csp6I GTAC 4 cut(s) 502, 1250, 1582, 2186
CspI CGGWCCG 1 cut(s) 60
CviQI GTAC 4 cut(s) 502, 1250, 1582, 2186
DpnI GATC 9 cut(s) 750, 1011, 1068, 1535, 2049, 2124, 2160, 2445, 2655
DpnII GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
DriI GACNNNNNGTC 2 cut(s) 517, 2037
EaeI YGGCCR 3 cut(s) 776, 2382, 2418
Eam1104I CTCTTC 7 cut(s) 464, 527, 822, 1760, 2580, 2889, 3050
Eam1105I GACNNNNNGTC 2 cut(s) 517, 2037
EarI CTCTTC 7 cut(s) 464, 527, 822, 1760, 2580, 2889, 3050
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 2220
Eco24I GRGCYC 2 cut(s) 2102, 2142
Eco32I GATATC 2 cut(s) 544, 594
Eco47I GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2659, 2719
Eco57I CTGAAG 5 cut(s) 1706, 1788, 1793, 2193, 2541
Eco88I CYCGRG 1 cut(s) 2402
Eco91I GGTNACC 1 cut(s) 667
EcoO109I RGGNCCY 2 cut(s) 642, 1575
EcoO65I GGTNACC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 2479
EcoRII CCWGG 1 cut(s) 2110
EcoRV GATATC 2 cut(s) 544, 594
EcoT14I CCWWGG 1 cut(s) 2220
EcoT22I ATGCAT 2 cut(s) 697, 1744
EcoT38I GRGCYC 2 cut(s) 2102, 2142
ErhI CCWWGG 1 cut(s) 2220
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 3 cut(s) 135, 589, 628
FauI CCCGC 1 cut(s) 2277
FbaI TGATCA 1 cut(s) 1066
FblI GTMKAC 1 cut(s) 2256
Fnu4HI GCNGC 8 cut(s) 338, 947, 1605, 1686, 1689, 1727, 1980, 2535
FokI GGATG 4 cut(s) 1056, 1761, 2567, 2912
FriOI GRGCYC 2 cut(s) 2102, 2142
Fsp4HI GCNGC 8 cut(s) 338, 947, 1605, 1686, 1689, 1727, 1980, 2535
FspBI CTAG 2 cut(s) 992, 3027
GlaI GCGC 6 cut(s) 498, 974, 1027, 1719, 2335, 2533
GluI GCNGC 8 cut(s) 338, 947, 1605, 1686, 1689, 1727, 1980, 2535
GsuI CTGGAG 2 cut(s) 2133, 2973
HaeII RGCGCY 2 cut(s) 1029, 2535
HaeIII GGCC 7 cut(s) 93, 778, 1436, 1577, 2384, 2420, 2775
HapII CCGG 4 cut(s) 58, 117, 147, 2438
HgaI GACGC 2 cut(s) 1166, 2946
HhaI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2534
Hin6I GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2532
HinP1I GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2532
HincII GTYRAC 2 cut(s) 997, 1159
HindII GTYRAC 2 cut(s) 997, 1159
HindIII AAGCTT 2 cut(s) 755, 2372
HpaI GTTAAC 2 cut(s) 997, 1159
HpaII CCGG 4 cut(s) 58, 117, 147, 2438
HphI GGTGA 8 cut(s) 442, 679, 1102, 1286, 2579, 2648, 2837, 2852
Hpy166II GTNNAC 9 cut(s) 997, 1105, 1159, 1231, 1672, 2203, 2257, 2351, 2933
Hpy8I GTNNAC 9 cut(s) 997, 1105, 1159, 1231, 1672, 2203, 2257, 2351, 2933
Hpy99I CGWCG 2 cut(s) 68, 2962
HpyAV CCTTC 9 cut(s) 85, 120, 1075, 1385, 1769, 1994, 2217, 2522, 2732
HpyCH4III ACNGT 4 cut(s) 984, 2185, 2636, 2735
HpyCH4IV ACGT 6 cut(s) 66, 504, 1126, 1216, 1500, 2550
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
HpySE526I ACGT 6 cut(s) 66, 504, 1126, 1216, 1500, 2550
HspAI GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2532
Ksp22I TGATCA 1 cut(s) 1066
KspAI GTTAAC 2 cut(s) 997, 1159
Kzo9I GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
LmnI GCTCC 5 cut(s) 187, 964, 970, 2176, 2954
Lsp1109I GCAGC 7 cut(s) 324, 933, 1591, 1675, 1738, 1966, 2521
MaeI CTAG 2 cut(s) 992, 3027
MaeII ACGT 6 cut(s) 66, 504, 1126, 1216, 1500, 2550
MaeIII GTNAC 7 cut(s) 667, 741, 903, 2029, 2636, 2825, 3045
MalI GATC 9 cut(s) 750, 1011, 1068, 1535, 2049, 2124, 2160, 2445, 2655
MboI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
MflI RGATCY 3 cut(s) 1009, 1533, 2443
MhlI GDGCHC 4 cut(s) 54, 2102, 2142, 2205
MlsI TGGCCA 1 cut(s) 2384
MluCI AATT 8 cut(s) 226, 1361, 1492, 1939, 2355, 2390, 2479, 2981
MluNI TGGCCA 1 cut(s) 2384
MlyI GAGTC 7 cut(s) 527, 1100, 1126, 1407, 1826, 1982, 2224
MmeI TCCRAC 2 cut(s) 87, 372
Mox20I TGGCCA 1 cut(s) 2384
Mph1103I ATGCAT 2 cut(s) 697, 1744
MroXI GAANNNNTTC 1 cut(s) 1851
MscI TGGCCA 1 cut(s) 2384
MslI CAYNNNNRTG 3 cut(s) 1747, 1883, 1926
Msp20I TGGCCA 1 cut(s) 2384
MspA1I CMGCKG 4 cut(s) 1477, 1604, 1979, 2272
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 4 cut(s) 58, 117, 147, 2438
MspR9I CCNGG 3 cut(s) 147, 2112, 2438
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 1 cut(s) 2112
MwoI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
NciI CCSGG 2 cut(s) 147, 2438
NcoI CCATGG 1 cut(s) 2220
NdeII GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
NlaIV GGNNCC 3 cut(s) 151, 644, 2401
NmeAIII GCCGAG 2 cut(s) 69, 2446
NmuCI GTSAC 5 cut(s) 667, 2029, 2636, 2825, 3045
NsiI ATGCAT 2 cut(s) 697, 1744
NspI RCATGY 2 cut(s) 1746, 2201
OliI CACNNNNGTG 1 cut(s) 1883
PciI ACATGT 1 cut(s) 2197
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 1851
PfeI GAWTC 8 cut(s) 8, 1349, 1367, 1546, 1952, 2407, 2606, 3023
PkrI GCNGC 8 cut(s) 339, 948, 1606, 1687, 1690, 1728, 1981, 2536
PleI GAGTC 7 cut(s) 526, 1100, 1126, 1406, 1825, 1981, 2224
PpsI GAGTC 7 cut(s) 526, 1100, 1126, 1406, 1825, 1981, 2224
Ppu21I YACGTR 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 642
PscI ACATGT 1 cut(s) 2197
PshBI ATTAAT 3 cut(s) 1257, 1517, 2393
Psp1406I AACGTT 1 cut(s) 1216
Psp5II RGGWCCY 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 2110
PspEI GGTNACC 1 cut(s) 667
PspGI CCWGG 1 cut(s) 2110
PspN4I GGNNCC 3 cut(s) 151, 644, 2401
PspPI GGNCC 9 cut(s) 60, 149, 642, 1199, 1453, 1575, 2399, 2659, 2719
PspPPI RGGWCCY 1 cut(s) 642
PsrI GAACNNNNNNTAC 2 cut(s) 2240, 2272
PstI CTGCAG 1 cut(s) 2539
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 3 cut(s) 1009, 1533, 2443
PvuII CAGCTG 2 cut(s) 1477, 1604
RsaI GTAC 4 cut(s) 503, 1251, 1583, 2187
RsaNI GTAC 4 cut(s) 502, 1250, 1582, 2186
RseI CAYNNNNRTG 3 cut(s) 1747, 1883, 1926
Rsr2I CGGWCCG 1 cut(s) 60
RsrII CGGWCCG 1 cut(s) 60
SatI GCNGC 8 cut(s) 338, 947, 1605, 1686, 1689, 1727, 1980, 2535
Sau3AI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2653
Sau96I GGNCC 9 cut(s) 60, 149, 642, 1199, 1453, 1575, 2399, 2659, 2719
ScaI AGTACT 1 cut(s) 2187
SchI GAGTC 7 cut(s) 527, 1100, 1126, 1407, 1826, 1982, 2224
ScrFI CCNGG 3 cut(s) 147, 2112, 2438
SduI GDGCHC 4 cut(s) 54, 2102, 2142, 2205
SfcI CTRYAG 1 cut(s) 2535
SinI GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2659, 2719
SmiMI CAYNNNNRTG 3 cut(s) 1747, 1883, 1926
SmlI CTYRAG 4 cut(s) 758, 881, 1423, 1835
SmoI CTYRAG 4 cut(s) 758, 881, 1423, 1835
SnaBI TACGTA 1 cut(s) 505
SpeI ACTAGT 1 cut(s) 991
Sse9I AATT 8 cut(s) 226, 1361, 1492, 1939, 2355, 2390, 2479, 2981
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 992, 3027
StyD4I CCNGG 3 cut(s) 145, 2110, 2436
StyI CCWWGG 1 cut(s) 2220
TaaI ACNGT 4 cut(s) 984, 2185, 2636, 2735
TaiI ACGT 6 cut(s) 69, 507, 1129, 1219, 1503, 2553
TaqI TCGA 6 cut(s) 132, 1224, 1527, 1855, 2625, 3069
TasI AATT 8 cut(s) 226, 1361, 1492, 1939, 2355, 2390, 2479, 2981
TatI WGTACW 2 cut(s) 1581, 2185
TauI GCSGC 1 cut(s) 1688
TfiI GAWTC 8 cut(s) 8, 1349, 1367, 1546, 1952, 2407, 2606, 3023
TscAI CASTG 5 cut(s) 279, 1092, 1564, 2710, 2740
TseFI GTSAC 5 cut(s) 667, 2029, 2636, 2825, 3045
TseI GCWGC 7 cut(s) 337, 946, 1604, 1688, 1726, 1979, 2534
Tsp45I GTSAC 5 cut(s) 667, 2029, 2636, 2825, 3045
TspGWI ACGGA 1 cut(s) 2950
TspRI CASTG 5 cut(s) 279, 1092, 1564, 2710, 2740
Vha464I CTTAAG 1 cut(s) 758
VneI GTGCAC 1 cut(s) 2201
VpaK11BI GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2659, 2719
VspI ATTAAT 3 cut(s) 1257, 1517, 2393
XapI RAATTY 4 cut(s) 226, 1492, 2479, 2981
XbaI TCTAGA 1 cut(s) 3026
XceI RCATGY 2 cut(s) 1746, 2201
XmiI GTMKAC 1 cut(s) 2256
XmnI GAANNNNTTC 1 cut(s) 1851
XspI CTAG 2 cut(s) 992, 3027
ZrmI AGTACT 1 cut(s) 2187
Zsp2I ATGCAT 2 cut(s) 697, 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.