Rroxscaffold_2G00099760

CCAAT enhancer-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008077
Physical Location & Seq
Forward (+)
21550849 .. 21559716
8868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099760.1

Sequence Viewer

Length: 3135 bp
ATGATGTCCTTGGGGAAGATTGTGGGAAAGAGTAAAGAGGTGGTGGTGGGATTCGAACCATCGTTGCGGCGCTTGAAGACAGAGCTTGAGAATCTGGTAACCCTAGTGGGCCTGAGTTCATCATCAGAAGCAAGTGAATTGGATCGTCTGTTGAACTTTTCAAGAGGTAGAGCCACAAATCCAGCAATGGCGGACAGAAAGAAATCTAAGAAACCCACAAACAACAACATCGAGCACCTCAAGACTGACATAGCCTCCTTCGCTTCCTCCCTCGGCCTCTCTACCTCTCTCCCTCCCTCTGATTCTTCAGGCTTCAACGATGCCCAATCTTTCGAAACCCCTTTTCCTTCACTCCCTGATGTCACCAGCAACAGTGACAAAGCCAAGACCTTTGAGAATCTTCCGAAGCTGCCGTTGATTTCAGCCACCAACATTGGAGTGTGGTACGAAGAAGCTGAGGAATTGGAAGGCAAAGTGGTTGGGAAGATGAAGAAAGCCGAGGCAAGGAGTGACAAGGAGTGGAGTGGTGAGGTGGCCAAGAAGCGAAAGCTTGGAGAGAGGTTGATGGCACAGTACACGGCGGATTACGAGGCTTCGAAAGGGAAGAGTGGGGATATCAAGCTGTTGCTGACCACACAGAGGTCAGGGACTGCTTCTGATAAGATTTCGGCGTTTTCGGTGTTGGTTGGGGATGATCCCATTGCCAATTTGAGGTCTTTGGATGCACTTTTAGGAATGGTAGCATCAAAAGTAGGGAAGCGATATGCATTTGCAGGTTTTGATGCATTGAGGGAGTTGTTTCTTACAAGTTTATTACCCGATCGTAAGCTTAAGAGCCTCTTACAAAGGCCTTTAAATGATCTTCCTGAGACAAAAGATGGTTATTCTCTTCTGCTTCTGTGGTATTGGGAGGAATGCTTGAAACAAAGGTATGAACGCTTTGTTATTGCTCTTGAGGAAGCATCAAGAGATATGTTACCTGAACTCAAAAACAAGGCATTGAAGACTATATATGTATTACTAAAGAGTAAATCAGAGCAAGAGCGAAGATTACTGTCTGCCCTAGTTAATAAACTGGGAGATCCTAAAAATAAGGGTGCTTCAGATGCTGATTTTCACTTATCAAACCTTTTGCGTGACCATCCAAACATGAAGGCTGTGGTGATTGATGAGGTGGACTCTTTTCTCTTTCGGCCGCATTTAAACATACAAGCAAAGTACCATGCGGTTAACTTTTTGAGCCAAATTCAACTAAGAAATAAAGGAGATGGACCAAAGGTGGCAAAAAGTTTGGTAGATGTATATTTTGCACTTTTCAAGGTACTGATTTCTGAGGCAGGTGGAGGTGAAAAGACTGAAAAGAACGAGAAAGTAGGGGGTAAAAAACCAGCCGGTTCTCGTAAGGATGGCAAAGGCAATAGTTCATCAGATTCTCATTTTGAATTGGATTCACGTCTTTTATCAGCTCTATTAATGGGAGTCAATAGAGCATTCCCATATGTTTCGAAGAATGAGGCTGATGACGTTGTTGAGGCCCAAACACCAACGCTTTTCCATTTGGTTCATTCTACTAACTTCAATGTAGGAGTTCAAGCACTGATGCTTCTTCATCATATCTCATCTAAGAATCAAATTGTCAGTGATAGGTTTTACCGAGCCTTGTACTCAAAGCTACTCCTTCCAGCTGCCATGAATACTTCCAAGGCTGAAACATTTATTGGACTTCTTCTGAGAGCAATGAAATCTGATGTCAATGTGAAGCGTACAGCTGCTTTTGCAAAACGTCTACTGCAGGTCGCCCTTCAACAGCCGCCTCAATATGCATGTGGATGCCTCTTTCTTCTCTCTGAAGTTCTAAAAGCACGGCCTCCCTTGTGGAACATGGTGCTCCAAAATGAGTCAGTTGATGATGACCTTGAGCATTTTGAAGATGTGATAGAAGAAACCGATAACGAACCCAGCCCTGTCCTGGAGAAACAAGAAGTTGAACTTGTTCATAACAGTGATGAAAAAAGTGATACCGTCAATTCCGATCATGATTCTTCAGAAGATGATATTGACTCTTCCGCCTCATATTCTGAAGATGAAGCTTCCGATGAAGCAGATGAGTTTCATTTTAGAAATGATTCAAAGGGCTCCAAGACGGTGCCTGATAGTAGCATGCAGCAACCACAAGTAGTATCCTCTGAGAAGACTTCACTACCTGGAGGCTATGATCCACGACACAGAGAGCCCTCTTACTGCAATGCAGATCGTGTAAGCTGGTGGGAGTTGACAGTACTTGCTTCACACGTGCACCCTTCAGTTTCTACCATGGCAAAGACGCTTCTCTCTGGGGCGAATATTGTCTATAATGGAAACCCACTGAATGACCTATCACTTACTGCTTTTCTGGACAAGTTTATGGAGAAAAAACCAAAGGCAAGCACATGGCATGGTGGTTCGCAAATTGAACCTGCGAAAAAGCTTGACATGACCAACCGATTCATAGGACCAGAAATCCTCTCATTGGCTGAAGAAGATGTACCTGCAGAAGATCTCGTCTTTCACAAGTTCTACATGAATAAAATGAATACTTCGAAGAAACCAAAGAAGAAGAAGAAGAAGAAGGCAACAACAGAAGATGAAGATGCCGCGGAGTTGTTTGATGTGGATGGTGGTGGTGATGATGAGAGTGATAATGAAGAGATTGACAATATGCTGGATTCTGCTGGTGTTGCCATGGATGCAGATGGCGACTATGATTATGATGATCTAGACCAAGTAGCCAATGATGACGATGAAGACTTGGTTGGTAATGTTAGTGATGTCAGTGATGCTGATCTTGACCTGCCCTCAGATATTGCAGAGGGTGAAGATTTTGATACCACTGCAAACAGTGATGAAAGTGATGATGGCGATGATGCTATTGATATAGGAGATGTTGATGACGACGAGGTTGAGGATGAGGATGAGGATGTCAACATTGAGAGTGTCCAGGGGAAAAGTAAACGGAAGCGTGGGAAGGCTGCAGCTTCCCCATTTGCGAGTCTGGAAGAATATGAGCATTTGTTTAACGAGGACGCCCCTGCTGAGAAGGAATCTAAAAGCAAGAAGAAATCCAAGCCCCGAAAGAAGAGAAAGTCGTCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

1044

Amino Acids

115.61

Weight (kDa)

4.98

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 530 - 773 4.6e-44 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1328
Acc36I ACCTGC 6 cut(s) 764, 1328, 1783, 2464, 2536, 2838
AccB1I GGYRCC 1 cut(s) 2146
AccI GTMKAC 1 cut(s) 1786
AccII CGCG 1 cut(s) 2636
AciI CCGC 9 cut(s) 67, 191, 581, 1196, 1226, 1811, 2067, 2634, 2636
AclWI GGATC 4 cut(s) 150, 689, 1076, 2210
AcoI YGGCCR 2 cut(s) 534, 1193
AcsI RAATTY 1 cut(s) 1245
AcuI CTGAAG 7 cut(s) 291, 1086, 1869, 2028, 2100, 2286, 2535
AcvI CACGTG 1 cut(s) 2293
AcyI GRCGYC 1 cut(s) 3063
AfaI GTAC 8 cut(s) 446, 575, 1220, 1323, 1664, 1765, 2280, 2526
AfiI CCNNNNNNNGG 5 cut(s) 504, 639, 711, 1969, 2509
AflII CTTAAG 1 cut(s) 830
AflIII ACRYGT 1 cut(s) 2290
AjiI CACGTC 1 cut(s) 1454
AjnI CCWGG 3 cut(s) 1968, 2203, 2976
AjuI GAANNNNNNNTTGG 4 cut(s) 1701, 1733, 2775, 2807
Alw21I GWGCWC 3 cut(s) 237, 1890, 2298
Alw26I GTCTC 1 cut(s) 863
Alw44I GTGCAC 1 cut(s) 2294
AlwI GGATC 4 cut(s) 150, 689, 1076, 2210
AlwNI CAGNNNCTG 2 cut(s) 650, 1109
AoxI GGCC 7 cut(s) 109, 274, 534, 848, 1193, 1533, 1865
ApaLI GTGCAC 1 cut(s) 2294
ApeKI GCWGC 6 cut(s) 409, 1685, 1769, 2164, 3008, 3011
ApoI RAATTY 1 cut(s) 1245
AseI ATTAAT 1 cut(s) 1472
Asp700I GAANNNNTTC 2 cut(s) 1992, 2125
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 109, 1271, 1534, 2492
AsuHPI GGTGA 6 cut(s) 355, 539, 1174, 1358, 2675, 2864
AsuII TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
AvaII GGWCC 2 cut(s) 1271, 2492
BaeGI GKGCMC 1 cut(s) 2298
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 1 cut(s) 2146
BanII GRGCYC 2 cut(s) 2138, 2235
BarI GAAGNNNNNNTAC 2 cut(s) 2508, 2540
BbrPI CACGTG 1 cut(s) 2293
BbsI GAAGAC 4 cut(s) 83, 1010, 2198, 2790
Bbv12I GWGCWC 3 cut(s) 237, 1890, 2298
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 6 cut(s) 396, 1672, 1756, 2176, 2995, 3023
BccI CCATC 9 cut(s) 67, 559, 872, 1149, 1262, 1400, 2648, 2726, 2888
BceAI ACGGC 3 cut(s) 397, 594, 1880
BciT130I CCWGG 3 cut(s) 1970, 2205, 2978
BciVI GTATCC 1 cut(s) 2191
BcoDI GTCTC 1 cut(s) 863
BfaI CTAG 3 cut(s) 104, 1064, 2756
BfmI CTRYAG 3 cut(s) 1790, 2529, 3009
BfoI RGCGCY 1 cut(s) 73
BfrI CTTAAG 1 cut(s) 830
BfuAI ACCTGC 6 cut(s) 764, 1328, 1783, 2464, 2536, 2838
BfuI GTATCC 1 cut(s) 2191
BglII AGATCT 1 cut(s) 2536
BmcAI AGTACT 1 cut(s) 2280
Bme1390I CCNGG 3 cut(s) 1970, 2205, 2978
Bme18I GGWCC 2 cut(s) 1271, 2492
BmgBI CACGTC 1 cut(s) 1454
BmgT120I GGNCC 4 cut(s) 109, 1271, 1534, 2492
BmiI GGNNCC 2 cut(s) 2137, 2148
BmrFI CCNGG 3 cut(s) 1970, 2205, 2978
BmrI ACTGGG 1 cut(s) 1085
BmuI ACTGGG 1 cut(s) 1085
BpiI GAAGAC 4 cut(s) 83, 1010, 2198, 2790
BplI GAGNNNNNCTC 2 cut(s) 1163, 1195
BpmI CTGGAG 2 cut(s) 1991, 2226
Bpu10I CCTNAGC 1 cut(s) 456
Bpu14I TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
BpuEI CTTGAG 4 cut(s) 107, 224, 974, 1937
BsaAI YACGTR 1 cut(s) 2293
BsaBI GATNNNNATC 1 cut(s) 2820
BsaHI GRCGYC 1 cut(s) 3063
BsaJI CCNNGG 8 cut(s) 9, 271, 498, 1701, 2313, 2634, 2721, 2977
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 5 cut(s) 504, 639, 711, 1969, 2509
Bse118I RCCGGY 1 cut(s) 1391
Bse1I ACTGG 1 cut(s) 1080
Bse3DI GCAATG 4 cut(s) 192, 699, 1743, 2251
Bse8I GATNNNNATC 1 cut(s) 2820
BseBI CCWGG 3 cut(s) 1970, 2205, 2978
BseDI CCNNGG 8 cut(s) 9, 271, 498, 1701, 2313, 2634, 2721, 2977
BseJI GATNNNNATC 1 cut(s) 2820
BseLI CCNNNNNNNGG 5 cut(s) 504, 639, 711, 1969, 2509
BseMI GCAATG 4 cut(s) 192, 699, 1743, 2251
BseMII CTCAG 8 cut(s) 104, 447, 858, 1323, 1721, 2178, 2850, 3063
BseNI ACTGG 1 cut(s) 1080
BseSI GKGCMC 1 cut(s) 2298
BseX3I CGGCCG 1 cut(s) 1193
BseXI GCAGC 6 cut(s) 396, 1672, 1756, 2176, 2995, 3023
BseYI CCCAGC 1 cut(s) 1958
Bsh1236I CGCG 1 cut(s) 2636
Bsh1285I CGRYCG 2 cut(s) 823, 1196
BshFI GGCC 7 cut(s) 111, 276, 536, 850, 1195, 1535, 1867
BshNI GGYRCC 1 cut(s) 2146
BsiEI CGRYCG 2 cut(s) 823, 1196
BsiHKAI GWGCWC 3 cut(s) 237, 1890, 2298
BsiSI CCGG 1 cut(s) 1392
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 5 cut(s) 504, 639, 711, 1969, 2509
BsmAI GTCTC 1 cut(s) 863
BsmFI GGGAC 1 cut(s) 661
BsmI GAATGC 2 cut(s) 920, 1490
BsnI GGCC 7 cut(s) 111, 276, 536, 850, 1195, 1535, 1867
Bsp119I TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
Bsp1286I GDGCHC 5 cut(s) 237, 1890, 2138, 2235, 2298
Bsp19I CCATGG 2 cut(s) 2313, 2721
BspACI CCGC 9 cut(s) 67, 191, 581, 1196, 1226, 1811, 2067, 2634, 2636
BspANI GGCC 7 cut(s) 111, 276, 536, 850, 1195, 1535, 1867
BspCNI CTCAG 8 cut(s) 105, 448, 859, 1324, 1722, 2179, 2849, 3064
BspFNI CGCG 1 cut(s) 2636
BspHI TCATGA 1 cut(s) 2035
BspLI GGNNCC 2 cut(s) 2137, 2148
BspMAI CTGCAG 3 cut(s) 1794, 2533, 3013
BspMI ACCTGC 6 cut(s) 764, 1328, 1783, 2464, 2536, 2838
BspPI GGATC 4 cut(s) 150, 689, 1076, 2210
BspT104I TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
BspT107I GGYRCC 1 cut(s) 2146
BspTI CTTAAG 1 cut(s) 830
BsrDI GCAATG 4 cut(s) 192, 699, 1743, 2251
BsrFI RCCGGY 1 cut(s) 1391
BsrI ACTGG 1 cut(s) 1080
BssAI RCCGGY 1 cut(s) 1391
BssECI CCNNGG 8 cut(s) 9, 271, 498, 1701, 2313, 2634, 2721, 2977
BssNI GRCGYC 1 cut(s) 3063
BssT1I CCWWGG 4 cut(s) 9, 1701, 2313, 2721
Bst2UI CCWGG 3 cut(s) 1970, 2205, 2978
Bst4CI ACNGT 8 cut(s) 374, 573, 1056, 2003, 2023, 2146, 2278, 2879
Bst6I CTCTTC 5 cut(s) 599, 894, 2068, 2679, 3110
BstACI GRCGYC 1 cut(s) 3063
BstAFI CTTAAG 1 cut(s) 830
BstBAI YACGTR 1 cut(s) 2293
BstBI TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
BstC8I GCNNGC 2 cut(s) 2162, 2425
BstDSI CCRYGG 3 cut(s) 2313, 2634, 2721
BstEII GGTNACC 1 cut(s) 97
BstFNI CGCG 1 cut(s) 2636
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 863
BstMCI CGRYCG 2 cut(s) 823, 1196
BstMWI GCNNNNNNNGC 5 cut(s) 260, 1106, 1775, 2717, 2726
BstNI CCWGG 3 cut(s) 1970, 2205, 2978
BstNSI RCATGY 2 cut(s) 1827, 2164
BstPI GGTNACC 1 cut(s) 97
BstSCI CCNGG 3 cut(s) 1968, 2203, 2976
BstSFI CTRYAG 3 cut(s) 1790, 2529, 3009
BstSLI GKGCMC 1 cut(s) 2298
BstUI CGCG 1 cut(s) 2636
BstV1I GCAGC 6 cut(s) 396, 1672, 1756, 2176, 2995, 3023
BstV2I GAAGAC 4 cut(s) 83, 1010, 2198, 2790
BstX2I RGATCY 2 cut(s) 1081, 2536
BstYI RGATCY 2 cut(s) 1081, 2536
BstZI CGGCCG 1 cut(s) 1193
BsuI GTATCC 1 cut(s) 2191
BsuRI GGCC 7 cut(s) 111, 276, 536, 850, 1195, 1535, 1867
BtgI CCRYGG 3 cut(s) 2313, 2634, 2721
BtgZI GCGATG 1 cut(s) 2913
BtrI CACGTC 1 cut(s) 1454
BtsI GCAGTG 1 cut(s) 2868
BtsIMutI CAGTG 8 cut(s) 379, 1595, 1645, 2008, 2363, 2818, 2868, 2884
BveI ACCTGC 6 cut(s) 764, 1328, 1783, 2464, 2536, 2838
Cac8I GCNNGC 2 cut(s) 2162, 2425
CaiI CAGNNNCTG 2 cut(s) 650, 1109
CciI TCATGA 1 cut(s) 2035
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 1391
Cfr13I GGNCC 4 cut(s) 109, 1271, 1534, 2492
Cfr42I CCGCGG 1 cut(s) 2637
CseI GACGC 2 cut(s) 2332, 3071
Csp6I GTAC 8 cut(s) 445, 574, 1219, 1322, 1663, 1764, 2279, 2525
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviQI GTAC 8 cut(s) 445, 574, 1219, 1322, 1663, 1764, 2279, 2525
DraI TTTAAA 2 cut(s) 855, 1203
EaeI YGGCCR 2 cut(s) 534, 1193
EagI CGGCCG 1 cut(s) 1193
Eam1104I CTCTTC 5 cut(s) 599, 894, 2068, 2679, 3110
EarI CTCTTC 5 cut(s) 599, 894, 2068, 2679, 3110
EciI GGCGGA 3 cut(s) 206, 596, 2056
EclXI CGGCCG 1 cut(s) 1193
Eco130I CCWWGG 4 cut(s) 9, 1701, 2313, 2721
Eco147I AGGCCT 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 2138, 2235
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 1271, 2492
Eco52I CGGCCG 1 cut(s) 1193
Eco57I CTGAAG 7 cut(s) 291, 1086, 1869, 2028, 2100, 2286, 2535
Eco72I CACGTG 1 cut(s) 2293
Eco91I GGTNACC 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 97
EcoRII CCWGG 3 cut(s) 1968, 2203, 2976
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 4 cut(s) 9, 1701, 2313, 2721
EcoT22I ATGCAT 3 cut(s) 769, 787, 1825
EcoT38I GRGCYC 2 cut(s) 2138, 2235
ErhI CCWWGG 4 cut(s) 9, 1701, 2313, 2721
FalI AAGNNNNNCTT 4 cut(s) 824, 856, 1974, 2006
FaqI GGGAC 1 cut(s) 661
FauNDI CATATG 1 cut(s) 1498
FblI GTMKAC 1 cut(s) 1786
FriOI GRGCYC 2 cut(s) 2138, 2235
FspBI CTAG 3 cut(s) 104, 1064, 2756
GlaI GCGC 1 cut(s) 71
GsaI CCCAGC 1 cut(s) 1962
GsuI CTGGAG 2 cut(s) 1991, 2226
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 7 cut(s) 111, 276, 536, 850, 1195, 1535, 1867
HapII CCGG 1 cut(s) 1392
HgaI GACGC 2 cut(s) 2332, 3071
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 3063
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 1231, 2274, 2962
HindII GTYRAC 3 cut(s) 1231, 2274, 2962
HindIII AAGCTT 4 cut(s) 548, 827, 2088, 2465
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 1 cut(s) 1392
HphI GGTGA 6 cut(s) 355, 539, 1174, 1358, 2675, 2864
Hpy166II GTNNAC 8 cut(s) 576, 1177, 1231, 1787, 2274, 2296, 2962, 2990
Hpy8I GTNNAC 8 cut(s) 576, 1177, 1231, 1787, 2274, 2296, 2962, 2990
Hpy99I CGWCG 1 cut(s) 2936
HpyCH4III ACNGT 8 cut(s) 374, 573, 1056, 2003, 2023, 2146, 2278, 2879
HpyCH4IV ACGT 4 cut(s) 1453, 1524, 1783, 2292
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 1106, 1775, 2717, 2726
HpySE526I ACGT 4 cut(s) 1453, 1524, 1783, 2292
Hsp92I GRCGYC 1 cut(s) 3063
HspAI GCGC 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 1231
KspI CCGCGG 1 cut(s) 2637
LmnI GCTCC 2 cut(s) 1893, 2141
Lsp1109I GCAGC 6 cut(s) 396, 1672, 1756, 2176, 2995, 3023
MaeI CTAG 3 cut(s) 104, 1064, 2756
MaeII ACGT 4 cut(s) 1453, 1524, 1783, 2292
MaeIII GTNAC 6 cut(s) 97, 361, 374, 509, 975, 1136
MflI RGATCY 2 cut(s) 1081, 2536
MhlI GDGCHC 5 cut(s) 237, 1890, 2138, 2235, 2298
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 8 cut(s) 137, 461, 706, 1245, 1442, 1632, 2026, 2448
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 5 cut(s) 1172, 1488, 1907, 2054, 3037
Mox20I TGGCCA 1 cut(s) 536
Mph1103I ATGCAT 3 cut(s) 769, 787, 1825
MroXI GAANNNNTTC 2 cut(s) 1992, 2125
MscI TGGCCA 1 cut(s) 536
MseI TTAA 8 cut(s) 831, 854, 1068, 1202, 1230, 1472, 3054, 3133
MslI CAYNNNNRTG 3 cut(s) 437, 1828, 2001
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 3 cut(s) 1685, 1769, 2636
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 1 cut(s) 1392
MspR9I CCNGG 3 cut(s) 1970, 2205, 2978
Mva1269I GAATGC 2 cut(s) 920, 1490
MvaI CCWGG 3 cut(s) 1970, 2205, 2978
MvnI CGCG 1 cut(s) 2636
MwoI GCNNNNNNNGC 5 cut(s) 260, 1106, 1775, 2717, 2726
NcoI CCATGG 2 cut(s) 2313, 2721
NdeI CATATG 1 cut(s) 1498
NlaIV GGNNCC 2 cut(s) 2137, 2148
NmeAIII GCCGAG 2 cut(s) 252, 523
NmuCI GTSAC 4 cut(s) 361, 374, 509, 1136
NsiI ATGCAT 3 cut(s) 769, 787, 1825
NspI RCATGY 2 cut(s) 1827, 2164
NspV TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
PaeI GCATGC 1 cut(s) 2164
PagI TCATGA 1 cut(s) 2035
PaqCI CACCTGC 1 cut(s) 1328
PceI AGGCCT 1 cut(s) 850
PctI GAATGC 2 cut(s) 920, 1490
PdmI GAANNNNTTC 2 cut(s) 1992, 2125
PfoI TCCNGGA 1 cut(s) 1968
Ple19I CGATCG 1 cut(s) 823
PleI GAGTC 5 cut(s) 1172, 1487, 1906, 2054, 3036
PmaCI CACGTG 1 cut(s) 2293
PmlI CACGTG 1 cut(s) 2293
PpsI GAGTC 5 cut(s) 1172, 1487, 1906, 2054, 3036
Ppu21I YACGTR 1 cut(s) 2293
PshBI ATTAAT 1 cut(s) 1472
Psp6I CCWGG 3 cut(s) 1968, 2203, 2976
PspCI CACGTG 1 cut(s) 2293
PspEI GGTNACC 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 1958
PspGI CCWGG 3 cut(s) 1968, 2203, 2976
PspN4I GGNNCC 2 cut(s) 2137, 2148
PspPI GGNCC 4 cut(s) 109, 1271, 1534, 2492
PstI CTGCAG 3 cut(s) 1794, 2533, 3013
PstNI CAGNNNCTG 2 cut(s) 650, 1109
PsuI RGATCY 2 cut(s) 1081, 2536
PvuI CGATCG 1 cut(s) 823
PvuII CAGCTG 2 cut(s) 1685, 1769
RsaI GTAC 8 cut(s) 446, 575, 1220, 1323, 1664, 1765, 2280, 2526
RsaNI GTAC 8 cut(s) 445, 574, 1219, 1322, 1663, 1764, 2279, 2525
RseI CAYNNNNRTG 3 cut(s) 437, 1828, 2001
SacII CCGCGG 1 cut(s) 2637
SaqAI TTAA 8 cut(s) 831, 854, 1068, 1202, 1230, 1472, 3054, 3133
Sau96I GGNCC 4 cut(s) 109, 1271, 1534, 2492
ScaI AGTACT 1 cut(s) 2280
SchI GAGTC 5 cut(s) 1172, 1488, 1907, 2054, 3037
ScrFI CCNGG 3 cut(s) 1970, 2205, 2978
SduI GDGCHC 5 cut(s) 237, 1890, 2138, 2235, 2298
SfcI CTRYAG 3 cut(s) 1790, 2529, 3009
Sfr303I CCGCGG 1 cut(s) 2637
SfuI TTCGAA 5 cut(s) 54, 333, 596, 1505, 2579
SgrBI CCGCGG 1 cut(s) 2637
SinI GGWCC 2 cut(s) 1271, 2492
SmiMI CAYNNNNRTG 3 cut(s) 437, 1828, 2001
SmlI CTYRAG 5 cut(s) 86, 239, 830, 953, 1916
SmoI CTYRAG 5 cut(s) 86, 239, 830, 953, 1916
SphI GCATGC 1 cut(s) 2164
Sse9I AATT 8 cut(s) 137, 461, 706, 1245, 1442, 1632, 2026, 2448
SseBI AGGCCT 1 cut(s) 850
SsiI CCGC 9 cut(s) 67, 191, 581, 1196, 1226, 1811, 2067, 2634, 2636
SspI AATATT 1 cut(s) 2344
SspMI CTAG 3 cut(s) 104, 1064, 2756
StuI AGGCCT 1 cut(s) 850
StyD4I CCNGG 3 cut(s) 1968, 2203, 2976
StyI CCWWGG 4 cut(s) 9, 1701, 2313, 2721
TaaI ACNGT 8 cut(s) 374, 573, 1056, 2003, 2023, 2146, 2278, 2879
TaiI ACGT 4 cut(s) 1456, 1527, 1786, 2295
TaqI TCGA 6 cut(s) 54, 231, 333, 596, 1505, 2579
TasI AATT 8 cut(s) 137, 461, 706, 1245, 1442, 1632, 2026, 2448
TatI WGTACW 3 cut(s) 573, 1662, 2278
TauI GCSGC 4 cut(s) 70, 1198, 1813, 2636
Tru1I TTAA 8 cut(s) 831, 854, 1068, 1202, 1230, 1472, 3054, 3133
Tru9I TTAA 8 cut(s) 831, 854, 1068, 1202, 1230, 1472, 3054, 3133
TscAI CASTG 8 cut(s) 379, 1602, 1645, 2008, 2370, 2818, 2875, 2884
TseFI GTSAC 4 cut(s) 361, 374, 509, 1136
TseI GCWGC 6 cut(s) 409, 1685, 1769, 2164, 3008, 3011
Tsp45I GTSAC 4 cut(s) 361, 374, 509, 1136
TspGWI ACGGA 1 cut(s) 3007
TspRI CASTG 8 cut(s) 379, 1602, 1645, 2008, 2370, 2818, 2875, 2884
Vha464I CTTAAG 1 cut(s) 830
VneI GTGCAC 1 cut(s) 2294
VpaK11BI GGWCC 2 cut(s) 1271, 2492
VspI ATTAAT 1 cut(s) 1472
XapI RAATTY 1 cut(s) 1245
XbaI TCTAGA 1 cut(s) 2755
XceI RCATGY 2 cut(s) 1827, 2164
XcmI CCANNNNNNNNNTGG 1 cut(s) 1966
XmiI GTMKAC 1 cut(s) 1786
XmnI GAANNNNTTC 2 cut(s) 1992, 2125
XspI CTAG 3 cut(s) 104, 1064, 2756
ZrmI AGTACT 1 cut(s) 2280
Zsp2I ATGCAT 3 cut(s) 769, 787, 1825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.