RchiOBHm_Chr2g0147011

CCAAT enhancer-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008077
Physical Location & Seq
Reverse (-)
64679921 .. 64686872
6952 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51668

Sequence Viewer

Length: 3048 bp
ATGGCGGACAGAAAGAAATCCAAGAAACCCACAAACAACAACATCGAGCACCTCAAGACTGACATAGCCTCCTTCGCTTCCTCCCTCGGCCTCTCTACCTCCCTCCCTCCCTCTGATTCTTCAGGCTTCAACGATGTCGATTTCCGCAACCCCAAAAAACCCCAACAGAGGCCTAAACCCTCAAAACCCCAGAATTCCAAACCCCAAAATCGAAACACCCAGAACTTCAAACCCAATGAAAGGCCCAATCTTTCGAAACCCCCTTTTCCTTCAGTCCCTGATGTCACCAGCAACAGTGAAAAAGCAAAGACCTTTGAGAATCTTCCGAAGCTGCCGTTGATTTCAGCCACCAACATTGGAGTGTGGTACGAAGAAGCTGAAGAATTGGAAGGCAAAGTGGTCGGGAAGATGAAGAAAGCCGAGGCAAGGAGTGACAAGGAGTGGAGTGGTGAGGTGGCCAAGAAGCGAAAGCTTGGAGAGAGGTTGATGGCACAGTACACGGCGGATTACGAGGCTTCGAAAGGGAAGAGTGGGGATATCAAGCTGTTGCTGACCACACAGAGGTCAGGGACTGCTTCTGATAAGATTTCGGCGTTTTCGGTGTTGGTTGGGGATGATCCCATTGCCAATTTGAGGTCTTTGGATGCACTTTTAGGAATGGTAGCATCAAAAGTAGGGAAGCGATACGCATTTGCAGGTTTTGATGCATTGAGGGAGTTGTTTCTTACAAGTTTATTACCCGATCGTAAGCTTAAGAGCCTCTTACAGCGGCCTTTAAATGATCTTCCTGAGACAAAAGATGGTTATTCTCTTCTGCTTCTGTGGTATTGGGAGGAATGCTTGAAACAAAGGTATGAACGCTTTGTTATTGCTCTTGAGGAAGCATCAAGAGATATGTTACCTGAACTAAAAAACAAGGCATTGAAGACTATATATGTATTACTAAAGAGTAAATCGGAGCAAGAGCGAAGATTACTGTCTGCCCTAGTTAATAAACTGGGAGATCCTAAAAATAAGGGTGCTTCAGATGCTGATTTTCACTTATCAAACCTTTTGCGTGACCATCCAAACATGAAGGCTGTGGTGATTGATGAGGTGGACTCTTTTCTCTTTCGGCCGCATTTAAACATACAAGCAAAGTACCATGCGGTTAACTTTTTGAGCCAAATTCAACTAAGAAATAAAGGAGATGGACCAAAGGTGGCAAAAAGTTTGGTAGATGTATATTTTGCACTTTTCAAGGTACTGATTTCTGAGGCAGGTGGAGGTGAAAAGACCGAAAAGAACGAGAAAGTAGGGGGTAAAAAACCAGCCGGTTCTCGTAAGGATGGCAAAGGCAATAGTTCATCAGATTCTCATTTTGAATTGGATTCACGTCTTCTATCAGCTCTATTAATGGGAGTCAATAGAGCATTCCCATATGTTTCAAAGAATGAGGCTGATGATGTTGTTGAGGCCCAAACACCAACGCTTTTCCATTTGGTTCATTCTACTAACTTCAATGTAGGAGTTCAAGCACTGATGCTTCTTCATCATATCTCATCTAAGAATCAAATTGTCAGTGATAGGTTTTACCGAGCCTTGTACTCAAAACTACTCCTTCCAGCTGCCATGAATACTTCCAAGGCTGAAACATTTATTGGACTTCTTCTGAGAGCAATGAAATCTGATGTCAATGTGAAGCGTACAGCTGCTTTTGCAAAACGTCTACTGCAGGTCGCCCTTCAACAGCCGCCTCAATATGCATGTGGATGCCTCTTTCTTCTCTCTGAAGTTCTAAAAGCACGGCCCCCCTTGTGGAACATGGTGCTCCAAAATGAGTCAGTTGATGATGACCTTGAGCATTTTGAAGATGTGATAGAAGAAACCGATAACGAACCCAGCCCTGTCCTGGAGAAACAAGAAGTTGAACTTGTTCATAACAGTGATGAAAAAAGTGATACCGTCAATTCCGATCATGATTCTTCAGAAGATGATATTGACTCTTCCGCCTCATATTCTGAAGATGAAGCTTCCGATGAAGCAGATGAGTTTCATTTTAGAAATGATTCAAAGGACTCCAAGACGGTGCCTGATAGTAGCGTGCAGCAACCACAAGTAGTGTCCTCTGAGAAGACTTCACTACCTGGAGGCTATGATCCACGACACAGAGAGCCCTCTTACTGCAATGCAGATCGTGTAAGCTGGTGGGAGTTGACAGTACTTGCTTCACATGTGCACCCTTCAGTTTCTACCATGGCAAAGACACTTCTCTCTGGGGCGAATATTGTCTATAATGGAAACCCACTGAATGACCTATCACTTACTGCTTTTCTGGACAAGTTTATGGAGAAAAAACCAAAGGCAAGCACATGGCATGGTGGTTCGCAAATTGAACCTGTGAAAAAGCTTGACATGACCAACCGATTTATAGGACCAGAAATCCTCTCATTGGCTGAAGAAGATGTACCTGCAGAAGATCTCGTCTTTCACAAGTTCTACATGAATAAAATGAATACTTCGAAGAAACCAAAGAAGAAGAAGAAGAAGGCAACAACAGAAGATGAAGATGCCGCGGAGTTGTTTGATGTGGATGGTGGTGGTGATGATGAGAGTGATAATGAAGAGATTGACAATATGCTGGATTCTGCTGGTATTGCCATGGATGCAGATGGCGACTATGATTATGATGATCTAGACCAAGTAGCCAATGACGACGATGAAGACTTGGTTGGTAATGTTAGTGATGTCAGTGATGCTGATCTTGACCTGCCCTCAGATATTGCAGAGGGTGAAGATTTTGATACCACTGCAGTTAGTGATGAAAGTGATGATGGCGATGATGCTATTGATATAGGAGATGTTGATGACGACGAGGTTGAGGACGAGGATGTCCATATTGAGAGTGTCCAGGGGAAAAGTAAACGGAAGCGTGGGAAGGCTGCAGCATCCCCATTTGCTAGTCTGGAAGAATATGAGCATTTGTTTAACGAGGACGCCCCTGCTGAGAAGGAATCTAAAAGCAAGAAGAAATCCAAGCCCCGAAAGAAGAGAAAGTCGTCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

1015

Amino Acids

112.9

Weight (kDa)

5.12

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 504 - 747 5.7e-44 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1250
AasI GACNNNNNNGTC 1 cut(s) 2870
Acc36I ACCTGC 5 cut(s) 686, 1250, 1705, 2458, 2757
AccB1I GGYRCC 1 cut(s) 2068
AccI GTMKAC 1 cut(s) 1708
AccII CGCG 1 cut(s) 2555
AclWI GGATC 3 cut(s) 611, 998, 2132
AcoI YGGCCR 2 cut(s) 456, 1115
AcsI RAATTY 2 cut(s) 193, 1167
AcuI CTGAAG 9 cut(s) 105, 255, 399, 1008, 1791, 1950, 2022, 2208, 2457
AcyI GRCGYC 1 cut(s) 2976
AfaI GTAC 8 cut(s) 368, 497, 1142, 1245, 1586, 1687, 2202, 2448
AfiI CCNNNNNNNGG 8 cut(s) 168, 240, 426, 561, 633, 1797, 1891, 2431
AflII CTTAAG 1 cut(s) 752
AflIII ACRYGT 1 cut(s) 2212
AjiI CACGTC 1 cut(s) 1376
AjnI CCWGG 3 cut(s) 1890, 2125, 2889
AjuI GAANNNNNNNTTGG 4 cut(s) 1623, 1655, 2694, 2726
Alw21I GWGCWC 3 cut(s) 51, 1812, 2220
Alw26I GTCTC 1 cut(s) 785
Alw44I GTGCAC 1 cut(s) 2216
AlwI GGATC 3 cut(s) 611, 998, 2132
AlwNI CAGNNNCTG 3 cut(s) 278, 572, 1031
AoxI GGCC 8 cut(s) 88, 170, 242, 456, 770, 1115, 1455, 1787
ApaLI GTGCAC 1 cut(s) 2216
ApeKI GCWGC 6 cut(s) 331, 1607, 1691, 2086, 2921, 2924
ApoI RAATTY 2 cut(s) 193, 1167
AseI ATTAAT 1 cut(s) 1394
Asp700I GAANNNNTTC 2 cut(s) 1914, 2047
AspS9I GGNCC 5 cut(s) 243, 1193, 1456, 1788, 2414
AsuHPI GGTGA 6 cut(s) 277, 461, 1096, 1280, 2594, 2783
AsuII TTCGAA 3 cut(s) 254, 518, 2501
AvaII GGWCC 2 cut(s) 1193, 2414
BaeGI GKGCMC 1 cut(s) 2220
BalI TGGCCA 1 cut(s) 458
BanI GGYRCC 1 cut(s) 2068
BanII GRGCYC 1 cut(s) 2157
BarI GAAGNNNNNNTAC 2 cut(s) 2430, 2462
BbsI GAAGAC 4 cut(s) 932, 1370, 2120, 2709
Bbv12I GWGCWC 3 cut(s) 51, 1812, 2220
BbvI GCAGC 6 cut(s) 318, 1594, 1678, 2098, 2908, 2936
BccI CCATC 8 cut(s) 481, 794, 1071, 1184, 1322, 2567, 2645, 2807
BceAI ACGGC 3 cut(s) 319, 516, 1802
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 3 cut(s) 1892, 2127, 2891
BcoDI GTCTC 1 cut(s) 785
BfaI CTAG 3 cut(s) 986, 2675, 2940
BfmI CTRYAG 4 cut(s) 1712, 2451, 2790, 2922
BfrI CTTAAG 1 cut(s) 752
BfuAI ACCTGC 5 cut(s) 686, 1250, 1705, 2458, 2757
BglII AGATCT 1 cut(s) 2458
BmcAI AGTACT 1 cut(s) 2202
Bme1390I CCNGG 3 cut(s) 1892, 2127, 2891
Bme18I GGWCC 2 cut(s) 1193, 2414
BmgBI CACGTC 1 cut(s) 1376
BmgT120I GGNCC 5 cut(s) 243, 1193, 1456, 1788, 2414
BmiI GGNNCC 2 cut(s) 1790, 2070
BmrFI CCNGG 3 cut(s) 1892, 2127, 2891
BmrI ACTGGG 1 cut(s) 1007
BmuI ACTGGG 1 cut(s) 1007
BpiI GAAGAC 4 cut(s) 932, 1370, 2120, 2709
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
BpmI CTGGAG 2 cut(s) 1913, 2148
Bpu14I TTCGAA 3 cut(s) 254, 518, 2501
BpuEI CTTGAG 3 cut(s) 38, 896, 1859
BsaBI GATNNNNATC 1 cut(s) 2739
BsaHI GRCGYC 1 cut(s) 2976
BsaJI CCNNGG 7 cut(s) 85, 420, 1623, 2235, 2553, 2640, 2890
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 8 cut(s) 168, 240, 426, 561, 633, 1797, 1891, 2431
Bse118I RCCGGY 1 cut(s) 1313
Bse1I ACTGG 1 cut(s) 1002
Bse3DI GCAATG 3 cut(s) 621, 1665, 2173
Bse8I GATNNNNATC 1 cut(s) 2739
BseBI CCWGG 3 cut(s) 1892, 2127, 2891
BseDI CCNNGG 7 cut(s) 85, 420, 1623, 2235, 2553, 2640, 2890
BseGI GGATG 9 cut(s) 619, 649, 1063, 1333, 1757, 2578, 2650, 2875, 2927
BseJI GATNNNNATC 1 cut(s) 2739
BseLI CCNNNNNNNGG 8 cut(s) 168, 240, 426, 561, 633, 1797, 1891, 2431
BseMI GCAATG 3 cut(s) 621, 1665, 2173
BseMII CTCAG 6 cut(s) 780, 1245, 1643, 2100, 2769, 2976
BseNI ACTGG 1 cut(s) 1002
BseSI GKGCMC 1 cut(s) 2220
BseX3I CGGCCG 1 cut(s) 1115
BseXI GCAGC 6 cut(s) 318, 1594, 1678, 2098, 2908, 2936
BseYI CCCAGC 1 cut(s) 1880
BsgI GTGCAG 1 cut(s) 2105
Bsh1236I CGCG 1 cut(s) 2555
Bsh1285I CGRYCG 2 cut(s) 745, 1118
BshFI GGCC 8 cut(s) 90, 172, 244, 458, 772, 1117, 1457, 1789
BshNI GGYRCC 1 cut(s) 2068
BsiEI CGRYCG 2 cut(s) 745, 1118
BsiHKAI GWGCWC 3 cut(s) 51, 1812, 2220
BsiSI CCGG 1 cut(s) 1314
BslFI GGGAC 2 cut(s) 260, 583
BslI CCNNNNNNNGG 8 cut(s) 168, 240, 426, 561, 633, 1797, 1891, 2431
BsmAI GTCTC 1 cut(s) 785
BsmFI GGGAC 2 cut(s) 260, 583
BsmI GAATGC 2 cut(s) 842, 1412
BsnI GGCC 8 cut(s) 90, 172, 244, 458, 772, 1117, 1457, 1789
Bsp119I TTCGAA 3 cut(s) 254, 518, 2501
Bsp1286I GDGCHC 4 cut(s) 51, 1812, 2157, 2220
Bsp19I CCATGG 2 cut(s) 2235, 2640
BspANI GGCC 8 cut(s) 90, 172, 244, 458, 772, 1117, 1457, 1789
BspCNI CTCAG 6 cut(s) 781, 1246, 1644, 2101, 2768, 2977
BspFNI CGCG 1 cut(s) 2555
BspHI TCATGA 1 cut(s) 1957
BspLI GGNNCC 2 cut(s) 1790, 2070
BspMAI CTGCAG 4 cut(s) 1716, 2455, 2794, 2926
BspMI ACCTGC 5 cut(s) 686, 1250, 1705, 2458, 2757
BspPI GGATC 3 cut(s) 611, 998, 2132
BspT104I TTCGAA 3 cut(s) 254, 518, 2501
BspT107I GGYRCC 1 cut(s) 2068
BspTI CTTAAG 1 cut(s) 752
BsrDI GCAATG 3 cut(s) 621, 1665, 2173
BsrFI RCCGGY 1 cut(s) 1313
BsrI ACTGG 1 cut(s) 1002
BssAI RCCGGY 1 cut(s) 1313
BssECI CCNNGG 7 cut(s) 85, 420, 1623, 2235, 2553, 2640, 2890
BssNI GRCGYC 1 cut(s) 2976
BssT1I CCWWGG 3 cut(s) 1623, 2235, 2640
Bst2UI CCWGG 3 cut(s) 1892, 2127, 2891
Bst4CI ACNGT 7 cut(s) 296, 495, 978, 1925, 1945, 2068, 2200
Bst6I CTCTTC 5 cut(s) 521, 816, 1990, 2598, 3023
BstACI GRCGYC 1 cut(s) 2976
BstAFI CTTAAG 1 cut(s) 752
BstBI TTCGAA 3 cut(s) 254, 518, 2501
BstC8I GCNNGC 2 cut(s) 2084, 2347
BstDEI CTNAG 8 cut(s) 789, 1175, 1254, 1545, 1652, 2109, 2755, 2985
BstDSI CCRYGG 3 cut(s) 2235, 2553, 2640
BstF5I GGATG 9 cut(s) 619, 649, 1063, 1333, 1757, 2578, 2650, 2875, 2927
BstFNI CGCG 1 cut(s) 2555
BstMAI GTCTC 1 cut(s) 785
BstMCI CGRYCG 2 cut(s) 745, 1118
BstMWI GCNNNNNNNGC 5 cut(s) 74, 1028, 1697, 2636, 2645
BstNI CCWGG 3 cut(s) 1892, 2127, 2891
BstNSI RCATGY 2 cut(s) 1749, 2216
BstSCI CCNGG 3 cut(s) 1890, 2125, 2889
BstSFI CTRYAG 4 cut(s) 1712, 2451, 2790, 2922
BstSLI GKGCMC 1 cut(s) 2220
BstUI CGCG 1 cut(s) 2555
BstV1I GCAGC 6 cut(s) 318, 1594, 1678, 2098, 2908, 2936
BstV2I GAAGAC 4 cut(s) 932, 1370, 2120, 2709
BstX2I RGATCY 2 cut(s) 1003, 2458
BstYI RGATCY 2 cut(s) 1003, 2458
BstZI CGGCCG 1 cut(s) 1115
BsuRI GGCC 8 cut(s) 90, 172, 244, 458, 772, 1117, 1457, 1789
BtgI CCRYGG 3 cut(s) 2235, 2553, 2640
BtgZI GCGATG 1 cut(s) 2832
BtrI CACGTC 1 cut(s) 1376
BtsCI GGATG 9 cut(s) 619, 649, 1063, 1333, 1757, 2578, 2650, 2875, 2927
BtsI GCAGTG 1 cut(s) 2787
BtsIMutI CAGTG 7 cut(s) 301, 1517, 1567, 1930, 2285, 2737, 2787
BveI ACCTGC 5 cut(s) 686, 1250, 1705, 2458, 2757
Cac8I GCNNGC 2 cut(s) 2084, 2347
CaiI CAGNNNCTG 3 cut(s) 278, 572, 1031
CciI TCATGA 1 cut(s) 1957
Cfr10I RCCGGY 1 cut(s) 1313
Cfr13I GGNCC 5 cut(s) 243, 1193, 1456, 1788, 2414
Cfr42I CCGCGG 1 cut(s) 2556
CseI GACGC 1 cut(s) 2984
Csp6I GTAC 8 cut(s) 367, 496, 1141, 1244, 1585, 1686, 2201, 2447
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviQI GTAC 8 cut(s) 367, 496, 1141, 1244, 1585, 1686, 2201, 2447
DdeI CTNAG 8 cut(s) 789, 1175, 1254, 1545, 1652, 2109, 2755, 2985
DraI TTTAAA 2 cut(s) 777, 1125
DrdI GACNNNNNNGTC 1 cut(s) 2870
DseDI GACNNNNNNGTC 1 cut(s) 2870
EaeI YGGCCR 2 cut(s) 456, 1115
EagI CGGCCG 1 cut(s) 1115
Eam1104I CTCTTC 5 cut(s) 521, 816, 1990, 2598, 3023
EarI CTCTTC 5 cut(s) 521, 816, 1990, 2598, 3023
EciI GGCGGA 3 cut(s) 20, 518, 1978
EclXI CGGCCG 1 cut(s) 1115
Eco130I CCWWGG 3 cut(s) 1623, 2235, 2640
Eco147I AGGCCT 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 2157
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 1193, 2414
Eco52I CGGCCG 1 cut(s) 1115
Eco57I CTGAAG 9 cut(s) 105, 255, 399, 1008, 1791, 1950, 2022, 2208, 2457
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 3 cut(s) 1890, 2125, 2889
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 3 cut(s) 1623, 2235, 2640
EcoT22I ATGCAT 2 cut(s) 709, 1747
EcoT38I GRGCYC 1 cut(s) 2157
ErhI CCWWGG 3 cut(s) 1623, 2235, 2640
FalI AAGNNNNNCTT 4 cut(s) 746, 778, 1896, 1928
FaqI GGGAC 2 cut(s) 260, 583
FauNDI CATATG 1 cut(s) 1420
FblI GTMKAC 1 cut(s) 1708
FokI GGATG 9 cut(s) 626, 656, 1050, 1340, 1764, 2585, 2657, 2882, 2914
FriOI GRGCYC 1 cut(s) 2157
FspBI CTAG 3 cut(s) 986, 2675, 2940
GsaI CCCAGC 1 cut(s) 1884
GsuI CTGGAG 2 cut(s) 1913, 2148
HaeIII GGCC 8 cut(s) 90, 172, 244, 458, 772, 1117, 1457, 1789
HapII CCGG 1 cut(s) 1314
HgaI GACGC 1 cut(s) 2984
Hin1I GRCGYC 1 cut(s) 2976
HincII GTYRAC 2 cut(s) 1153, 2196
HindII GTYRAC 2 cut(s) 1153, 2196
HindIII AAGCTT 4 cut(s) 470, 749, 2010, 2387
HpaI GTTAAC 1 cut(s) 1153
HpaII CCGG 1 cut(s) 1314
HphI GGTGA 6 cut(s) 277, 461, 1096, 1280, 2594, 2783
Hpy166II GTNNAC 7 cut(s) 498, 1099, 1153, 1709, 2196, 2218, 2903
Hpy8I GTNNAC 7 cut(s) 498, 1099, 1153, 1709, 2196, 2218, 2903
Hpy99I CGWCG 2 cut(s) 2699, 2855
HpyCH4III ACNGT 7 cut(s) 296, 495, 978, 1925, 1945, 2068, 2200
HpyCH4IV ACGT 2 cut(s) 1375, 1705
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 1028, 1697, 2636, 2645
HpyF3I CTNAG 8 cut(s) 789, 1175, 1254, 1545, 1652, 2109, 2755, 2985
HpySE526I ACGT 2 cut(s) 1375, 1705
Hsp92I GRCGYC 1 cut(s) 2976
KspAI GTTAAC 1 cut(s) 1153
KspI CCGCGG 1 cut(s) 2556
LmnI GCTCC 2 cut(s) 958, 1815
Lsp1109I GCAGC 6 cut(s) 318, 1594, 1678, 2098, 2908, 2936
MaeI CTAG 3 cut(s) 986, 2675, 2940
MaeII ACGT 2 cut(s) 1375, 1705
MaeIII GTNAC 4 cut(s) 283, 431, 897, 1058
MflI RGATCY 2 cut(s) 1003, 2458
MhlI GDGCHC 4 cut(s) 51, 1812, 2157, 2220
MlsI TGGCCA 1 cut(s) 458
MluCI AATT 8 cut(s) 193, 383, 628, 1167, 1364, 1554, 1948, 2370
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 5 cut(s) 1094, 1410, 1829, 1976, 2051
Mox20I TGGCCA 1 cut(s) 458
Mph1103I ATGCAT 2 cut(s) 709, 1747
MroXI GAANNNNTTC 2 cut(s) 1914, 2047
MscI TGGCCA 1 cut(s) 458
MseI TTAA 8 cut(s) 753, 776, 990, 1124, 1152, 1394, 2967, 3046
MslI CAYNNNNRTG 3 cut(s) 359, 1750, 1923
Msp20I TGGCCA 1 cut(s) 458
MspA1I CMGCKG 4 cut(s) 769, 1607, 1691, 2555
MspCI CTTAAG 1 cut(s) 752
MspI CCGG 1 cut(s) 1314
MspR9I CCNGG 3 cut(s) 1892, 2127, 2891
Mva1269I GAATGC 2 cut(s) 842, 1412
MvaI CCWGG 3 cut(s) 1892, 2127, 2891
MvnI CGCG 1 cut(s) 2555
MwoI GCNNNNNNNGC 5 cut(s) 74, 1028, 1697, 2636, 2645
NcoI CCATGG 2 cut(s) 2235, 2640
NdeI CATATG 1 cut(s) 1420
NlaIV GGNNCC 2 cut(s) 1790, 2070
NmeAIII GCCGAG 2 cut(s) 66, 445
NmuCI GTSAC 3 cut(s) 283, 431, 1058
NsiI ATGCAT 2 cut(s) 709, 1747
NspI RCATGY 2 cut(s) 1749, 2216
NspV TTCGAA 3 cut(s) 254, 518, 2501
PagI TCATGA 1 cut(s) 1957
PaqCI CACCTGC 1 cut(s) 1250
PceI AGGCCT 1 cut(s) 172
PciI ACATGT 1 cut(s) 2212
PctI GAATGC 2 cut(s) 842, 1412
PdmI GAANNNNTTC 2 cut(s) 1914, 2047
PfeI GAWTC 9 cut(s) 116, 319, 1352, 1370, 1549, 1961, 2048, 2624, 2993
PfoI TCCNGGA 1 cut(s) 1890
Ple19I CGATCG 1 cut(s) 745
PleI GAGTC 5 cut(s) 1094, 1409, 1828, 1976, 2051
PpsI GAGTC 5 cut(s) 1094, 1409, 1828, 1976, 2051
PscI ACATGT 1 cut(s) 2212
PshBI ATTAAT 1 cut(s) 1394
Psp6I CCWGG 3 cut(s) 1890, 2125, 2889
PspFI CCCAGC 1 cut(s) 1880
PspGI CCWGG 3 cut(s) 1890, 2125, 2889
PspN4I GGNNCC 2 cut(s) 1790, 2070
PspPI GGNCC 5 cut(s) 243, 1193, 1456, 1788, 2414
PstI CTGCAG 4 cut(s) 1716, 2455, 2794, 2926
PstNI CAGNNNCTG 3 cut(s) 278, 572, 1031
PsuI RGATCY 2 cut(s) 1003, 2458
PvuI CGATCG 1 cut(s) 745
PvuII CAGCTG 2 cut(s) 1607, 1691
RsaI GTAC 8 cut(s) 368, 497, 1142, 1245, 1586, 1687, 2202, 2448
RsaNI GTAC 8 cut(s) 367, 496, 1141, 1244, 1585, 1686, 2201, 2447
RseI CAYNNNNRTG 3 cut(s) 359, 1750, 1923
SacII CCGCGG 1 cut(s) 2556
SaqAI TTAA 8 cut(s) 753, 776, 990, 1124, 1152, 1394, 2967, 3046
Sau96I GGNCC 5 cut(s) 243, 1193, 1456, 1788, 2414
ScaI AGTACT 1 cut(s) 2202
SchI GAGTC 5 cut(s) 1094, 1410, 1829, 1976, 2051
ScrFI CCNGG 3 cut(s) 1892, 2127, 2891
SduI GDGCHC 4 cut(s) 51, 1812, 2157, 2220
SfcI CTRYAG 4 cut(s) 1712, 2451, 2790, 2922
Sfr303I CCGCGG 1 cut(s) 2556
SfuI TTCGAA 3 cut(s) 254, 518, 2501
SgrBI CCGCGG 1 cut(s) 2556
SinI GGWCC 2 cut(s) 1193, 2414
SmiMI CAYNNNNRTG 3 cut(s) 359, 1750, 1923
SmlI CTYRAG 4 cut(s) 53, 752, 875, 1838
SmoI CTYRAG 4 cut(s) 53, 752, 875, 1838
Sse9I AATT 8 cut(s) 193, 383, 628, 1167, 1364, 1554, 1948, 2370
SseBI AGGCCT 1 cut(s) 172
SspI AATATT 1 cut(s) 2266
SspMI CTAG 3 cut(s) 986, 2675, 2940
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 3 cut(s) 1890, 2125, 2889
StyI CCWWGG 3 cut(s) 1623, 2235, 2640
TaaI ACNGT 7 cut(s) 296, 495, 978, 1925, 1945, 2068, 2200
TaiI ACGT 2 cut(s) 1378, 1708
TaqI TCGA 6 cut(s) 45, 138, 211, 254, 518, 2501
TaqII GACCGA 1 cut(s) 1292
TasI AATT 8 cut(s) 193, 383, 628, 1167, 1364, 1554, 1948, 2370
TatI WGTACW 3 cut(s) 495, 1584, 2200
TauI GCSGC 4 cut(s) 772, 1120, 1735, 2555
TfiI GAWTC 9 cut(s) 116, 319, 1352, 1370, 1549, 1961, 2048, 2624, 2993
Tru1I TTAA 8 cut(s) 753, 776, 990, 1124, 1152, 1394, 2967, 3046
Tru9I TTAA 8 cut(s) 753, 776, 990, 1124, 1152, 1394, 2967, 3046
TscAI CASTG 7 cut(s) 301, 1524, 1567, 1930, 2292, 2737, 2794
TseFI GTSAC 3 cut(s) 283, 431, 1058
TseI GCWGC 6 cut(s) 331, 1607, 1691, 2086, 2921, 2924
Tsp45I GTSAC 3 cut(s) 283, 431, 1058
TspGWI ACGGA 1 cut(s) 2920
TspRI CASTG 7 cut(s) 301, 1524, 1567, 1930, 2292, 2737, 2794
Vha464I CTTAAG 1 cut(s) 752
VneI GTGCAC 1 cut(s) 2216
VpaK11BI GGWCC 2 cut(s) 1193, 2414
VspI ATTAAT 1 cut(s) 1394
XapI RAATTY 2 cut(s) 193, 1167
XbaI TCTAGA 1 cut(s) 2674
XceI RCATGY 2 cut(s) 1749, 2216
XcmI CCANNNNNNNNNTGG 1 cut(s) 1888
XmiI GTMKAC 1 cut(s) 1708
XmnI GAANNNNTTC 2 cut(s) 1914, 2047
XspI CTAG 3 cut(s) 986, 2675, 2940
ZrmI AGTACT 1 cut(s) 2202
Zsp2I ATGCAT 2 cut(s) 709, 1747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.