Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001962

Physical Location & Seq

Genomic Coordinates Forward (+)

27737796 .. 27743527

Gene Structure 5732 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1229400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGCTATAGAAACTATCCCAAACGACATCGTTTACCGGTCCAAACTCCCCGACATCCCCATCCCAAAACACCTCCCTCTCCACTCCTACTGCCTCCACAACAAAAACCACTCGAGCTCTAAGCCTTGTATCATCGACGGCGCCACCGGAGACATATACACCTTCGCCGATGTGGAACTTAACGCACGCAGAGTCGCGTCAGGACTCAACAAGCTTGGAATCCAACAAGGCGACGTCATCATGCTCTTGCTCCCCAACTCCCCAGCGTTCGCCTTTGCCTTCCTCGGAGCCTCCTTTCGCGGCGCTATGACAACCGCGGCGAACCCTTTCTTCACACCCGCCGAGATCTTAAAACAGGCCAAGGCCTCAAAAGCTAAACTCATCATCACCTTAGCGTGCTACTACGACAAGGTCAAGGACTTATCGTCATCAAGTGACGATGTTCACGACATTAAGCTAATGTGCGTCGACTCTCCGCCTGATCCGAGCTGTTTGCATTTCTCCGAGCTTCTTCAAGCTGATGAAAAGGACATGCCGGAGGTTGACATCAGCCCAGACGACGTCGTCGCTCTACCCTACTCGTCCGGGACGACAGGCTTGCCTAAGGGGGTGATGCTAACGCACAAGGGTCTTGTGACAAGCGTTGCTCAGCAGGTGGACGGGGAAAATCCCAACTTGTATTATAGCACCGACGACGTCATCTTATGCGTGCTGCCACTTTTTCATATTTATTCTTTGAACTCGGTATTGCTTTGTGGACTTAGAGCAGGAGCTGCCATTTTGATGATGAACAAGTTTGAGATTGTTTCTCTGTTGGGCTTGATCGACAAGTACAAGGTTAGTATTGCACCGATCGTGCCGCCGATCGTTTTGGCCATCGCCAAGTTTCCCGATCTTGATAAGTACGATTTGTCGTCGATTCGAGTGCTTAAGTGTGGAGGGGCACCCCTTGGGAAGGAGCTTGAGGATACTGTGAGAGCCAAGTTTCCCAATGTCACACTTGGTCAGGGATATGGAATGACAGAGGCAGGGCCAGTATTGACCATGTCATTGGCATTTGCCAAGCAACCCTTCGAGGTCAAACCAGGTGGATGTGGCACCGTCGTCCGAAATGCAGAGCTCAAGATCGTTGATCCTGAATCTGGTGCTTCTTTGCCACGCAACCAGCCTGGAGAGATTTGCATTAGAGGTGACCAGATCATGAAAGGTTATCTTAATGATCCGGAGTCGACAAGGACAACCATAGACAAGGAAGGTTGGCTACACACCGGTGATATAGGCTTCATTGATGATGATGATGAGCTCTTCATTGTTGATCGGTTGAAGGAACTGATCAAATACAAAGGGTTCCAAGTGGCCCCTGCTGAACTTGAAGCCTTGCTCATCACCCATCCTAGTGTTTCTGATGCTGCTGTTGTCCCAATGAAGGATGAGGCAGCTGGAGAGGTTCCGGTTGCATTTGTAGTAAGGTCAAATAATTCTCAGCTCACTGAGGACGAAGTCAAGCAATTTATCTCTAAACAGGTTGTATTTTATAAAAGAATAAATCGAGTATTTTTCATCGAAGCCATTCCGAAGTCACCGTCGGGCAAAATCTTGCGGAAGGACTTGAGAGCAAAGCTTGCTGCTGGGTTTCCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009987 GO:0016207 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019438 GO:0019748 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

548

Amino Acids

59.52

Weight (kDa)

5.59

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	37 - 405	2.7e-94	AMP-binding enzyme
AMP-binding_C	PF13193	456 - 531	2.6e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51680 AT1G51680 AT1G51680 AT3G21240 AT3G21240 AT3G21240
fragaria_vesca	FvH4_4g09340 FvH4_4g09340 FvH4_4g09340 FvH4_4g09340 FvH4_4g09340 FvH4_6g27940 FvH4_6g27940 FvH4_6g27940 FvH4_6g27940 FvH4_6g27940
malus_domestica	MD13G1257800.v1.1 MD17G1229400.v1.1
prunus_persica	Prupe.1G087900_v2.0.a1 Prupe.3G107300_v2.0.a1
pyrus_communis	pycom13g22250 pycom17g23190
rosa_chinensis	RchiOBHm_Chr2g0131501 RchiOBHm_Chr4g0402711 RchiOBHm_Chr5g0057551
rosa_laevigata	RLG00000009052 RLG00000019189
rosa_multiflora	Rmu_co7959539.1_g000001 Rmu_sc0000329.1_g000003 Rmu_sc0002230.1_g000020 Rmu_sc0002451.1_g000001 Rmu_sc0002808.1_g000009
rosa_roxburghii	Rroxscaffold_2G00112820 Rroxscaffold_5G00347470
rosa_rugosa	Rorug02G0297600 Rorug04G0037900
rosa_samantha	Rh2AG350900 Rh2BG357400 Rh2CG336600 Rh2DG375300 Rh4AG115500 Rh4CG122400 Rh4DG108000
rosa_wichuraiana	Rw2G028470 Rw4G009230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1536
AarI	CACCTGC	1 cut(s)	643
AasI	GACNNNNNNGTC	1 cut(s)	563
AatII	GACGTC	3 cut(s)	237, 564, 699
Acc36I	ACCTGC	1 cut(s)	643
AccB1I	GGYRCC	3 cut(s)	140, 943, 1097
AccI	GTMKAC	2 cut(s)	468, 1229
AccII	CGCG	3 cut(s)	197, 300, 317
AccIII	TCCGGA	1 cut(s)	1222
AciI	CCGC	7 cut(s)	300, 315, 317, 339, 476, 860, 1600
AclWI	GGATC	3 cut(s)	476, 1127, 1214
AcoI	YGGCCR	1 cut(s)	873
AcyI	GRCGYC	4 cut(s)	141, 234, 561, 696
AfaI	GTAC	2 cut(s)	833, 905
AfiI	CCNNNNNNNGG	3 cut(s)	955, 1142, 1426
AflII	CTTAAG	1 cut(s)	929
AgeI	ACCGGT	2 cut(s)	36, 1268
AgsI	TTSAA	4 cut(s)	515, 739, 1324, 1373
AhdI	GACNNNNNGTC	1 cut(s)	424
AjnI	CCWGG	2 cut(s)	1084, 1168
AjuI	GAANNNNNNNTTGG	2 cut(s)	1055, 1087
AleI	CACNNNNGTG	1 cut(s)	1269
Alw21I	GWGCWC	3 cut(s)	119, 1122, 1305
Alw26I	GTCTC	1 cut(s)	144
AlwI	GGATC	3 cut(s)	476, 1127, 1214
AlwNI	CAGNNNCTG	1 cut(s)	773
Ama87I	CYCGRG	1 cut(s)	112
Aor13HI	TCCGGA	1 cut(s)	1222
AoxI	GGCC	5 cut(s)	357, 363, 873, 1031, 1356
ApeKI	GCWGC	5 cut(s)	712, 773, 1409, 1436, 1625
ArsI	GACNNNNNNTTYG	2 cut(s)	1568, 1600
AsiGI	ACCGGT	2 cut(s)	36, 1268
Asp700I	GAANNNNTTC	2 cut(s)	326, 1569
AspLEI	GCGC	2 cut(s)	143, 305
AspS9I	GGNCC	3 cut(s)	39, 1031, 1357
AsuC2I	CCSGG	1 cut(s)	586
AsuHPI	GGTGA	6 cut(s)	379, 622, 1202, 1283, 1378, 1572
AvaI	CYCGRG	1 cut(s)	112
AvaII	GGWCC	1 cut(s)	39
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	1 cut(s)	946
BalI	TGGCCA	1 cut(s)	875
BanI	GGYRCC	3 cut(s)	140, 943, 1097
BanII	GRGCYC	3 cut(s)	119, 1122, 1305
BarI	GAAGNNNNNNTAC	2 cut(s)	1245, 1277
Bbv12I	GWGCWC	3 cut(s)	119, 1122, 1305
BbvI	GCAGC	5 cut(s)	699, 760, 1396, 1448, 1612
BccI	CCATC	3 cut(s)	68, 884, 1398
BceAI	ACGGC	1 cut(s)	154
BcgI	CGANNNNNNTGC	6 cut(s)	475, 509, 580, 614, 907, 941
BciT130I	CCWGG	2 cut(s)	1086, 1170
BciVI	GTATCC	1 cut(s)	961
BclI	TGATCA	1 cut(s)	1332
BcnI	CCSGG	1 cut(s)	586
BcoDI	GTCTC	1 cut(s)	144
BfaI	CTAG	1 cut(s)	1395
BfmI	CTRYAG	1 cut(s)	6
BfoI	RGCGCY	2 cut(s)	144, 306
BfrI	CTTAAG	1 cut(s)	929
BfuAI	ACCTGC	1 cut(s)	643
BfuI	GTATCC	1 cut(s)	961
BglII	AGATCT	1 cut(s)	345
BisI	GCNGC	8 cut(s)	301, 318, 713, 774, 860, 1410, 1437, 1626
BlpI	GCTNAGC	1 cut(s)	648
BlsI	GCNGC	8 cut(s)	302, 319, 714, 775, 861, 1411, 1438, 1627
Bme1390I	CCNGG	3 cut(s)	586, 1086, 1170
Bme18I	GGWCC	1 cut(s)	39
BmeRI	GACNNNNNGTC	1 cut(s)	424
BmeT110I	CYCGRG	1 cut(s)	112
BmgT120I	GGNCC	3 cut(s)	39, 1031, 1357
BmiI	GGNNCC	7 cut(s)	142, 289, 945, 1099, 1349, 1359, 1449
BmrFI	CCNGG	3 cut(s)	586, 1086, 1170
BmsI	GCATC	2 cut(s)	603, 1396
BpmI	CTGGAG	2 cut(s)	1191, 1461
Bpu10I	CCTNAGC	1 cut(s)	391
Bpu1102I	GCTNAGC	1 cut(s)	648
BpuEI	CTTGAG	3 cut(s)	983, 1106, 1630
BpuMI	CCSGG	1 cut(s)	586
BsaHI	GRCGYC	4 cut(s)	141, 234, 561, 696
BsaJI	CCNNGG	4 cut(s)	283, 315, 360, 949
BsaWI	WCCGGW	5 cut(s)	36, 146, 1222, 1268, 1450
Bsc4I	CCNNNNNNNGG	3 cut(s)	955, 1142, 1426
Bse118I	RCCGGY	2 cut(s)	36, 1268
Bse1I	ACTGG	1 cut(s)	1034
Bse21I	CCTNAGG	1 cut(s)	603
BseAI	TCCGGA	1 cut(s)	1222
BseBI	CCWGG	2 cut(s)	1086, 1170
BseDI	CCNNGG	4 cut(s)	283, 315, 360, 949
BseGI	GGATG	5 cut(s)	54, 60, 1097, 1390, 1435
BseLI	CCNNNNNNNGG	3 cut(s)	955, 1142, 1426
BseMII	CTCAG	3 cut(s)	662, 1482, 1496
BseNI	ACTGG	1 cut(s)	1034
BseSI	GKGCMC	1 cut(s)	946
BseXI	GCAGC	5 cut(s)	699, 760, 1396, 1448, 1612
BseYI	CCCAGC	2 cut(s)	262, 1628
Bsh1236I	CGCG	3 cut(s)	197, 300, 317
Bsh1285I	CGRYCG	2 cut(s)	855, 867
BshFI	GGCC	5 cut(s)	359, 365, 875, 1033, 1358
BshNI	GGYRCC	3 cut(s)	140, 943, 1097
BshTI	ACCGGT	2 cut(s)	36, 1268
BsiEI	CGRYCG	2 cut(s)	855, 867
BsiHKAI	GWGCWC	3 cut(s)	119, 1122, 1305
BsiHKCI	CYCGRG	1 cut(s)	112
BsiSI	CCGG	7 cut(s)	37, 147, 536, 585, 1223, 1269, 1451
BslFI	GGGAC	2 cut(s)	601, 1403
BslI	CCNNNNNNNGG	3 cut(s)	955, 1142, 1426
BsmAI	GTCTC	1 cut(s)	144
BsmFI	GGGAC	2 cut(s)	601, 1403
BsnI	GGCC	5 cut(s)	359, 365, 875, 1033, 1358
BsoBI	CYCGRG	1 cut(s)	112
Bsp1286I	GDGCHC	4 cut(s)	119, 946, 1122, 1305
Bsp13I	TCCGGA	1 cut(s)	1222
Bsp1720I	GCTNAGC	1 cut(s)	648
BspACI	CCGC	7 cut(s)	300, 315, 317, 339, 476, 860, 1600
BspANI	GGCC	5 cut(s)	359, 365, 875, 1033, 1358
BspCNI	CTCAG	3 cut(s)	661, 1483, 1495
BspEI	TCCGGA	1 cut(s)	1222
BspFNI	CGCG	3 cut(s)	197, 300, 317
BspHI	TCATGA	1 cut(s)	1200
BspLI	GGNNCC	7 cut(s)	142, 289, 945, 1099, 1349, 1359, 1449
BspMI	ACCTGC	1 cut(s)	643
BspPI	GGATC	3 cut(s)	476, 1127, 1214
BspQI	GCTCTTC	1 cut(s)	1310
BspT107I	GGYRCC	3 cut(s)	140, 943, 1097
BspTI	CTTAAG	1 cut(s)	929
BsrFI	RCCGGY	2 cut(s)	36, 1268
BsrI	ACTGG	1 cut(s)	1034
BssAI	RCCGGY	2 cut(s)	36, 1268
BssECI	CCNNGG	4 cut(s)	283, 315, 360, 949
BssNI	GRCGYC	4 cut(s)	141, 234, 561, 696
BssT1I	CCWWGG	2 cut(s)	360, 949
Bst2UI	CCWGG	2 cut(s)	1086, 1170
Bst4CI	ACNGT	3 cut(s)	973, 1102, 1584
Bst6I	CTCTTC	1 cut(s)	1310
BstACI	GRCGYC	4 cut(s)	141, 234, 561, 696
BstAFI	CTTAAG	1 cut(s)	929
BstAPI	GCANNNNNTGC	2 cut(s)	773, 1622
BstC8I	GCNNGC	5 cut(s)	187, 397, 599, 710, 1623
BstDEI	CTNAG	7 cut(s)	120, 391, 603, 648, 761, 1482, 1491
BstDSI	CCRYGG	1 cut(s)	315
BstEII	GGTNACC	1 cut(s)	1190
BstENI	CCTNNNNNAGG	1 cut(s)	953
BstF5I	GGATG	5 cut(s)	54, 60, 1097, 1390, 1435
BstFNI	CGCG	3 cut(s)	197, 300, 317
BstH2I	RGCGCY	2 cut(s)	144, 306
BstHHI	GCGC	2 cut(s)	143, 305
BstMAI	GTCTC	1 cut(s)	144
BstMCI	CGRYCG	2 cut(s)	855, 867
BstMWI	GCNNNNNNNGC	3 cut(s)	371, 773, 1622
BstNI	CCWGG	2 cut(s)	1086, 1170
BstNSI	RCATGY	1 cut(s)	535
BstPI	GGTNACC	1 cut(s)	1190
BstSCI	CCNGG	3 cut(s)	584, 1084, 1168
BstSFI	CTRYAG	1 cut(s)	6
BstSLI	GKGCMC	1 cut(s)	946
BstUI	CGCG	3 cut(s)	197, 300, 317
BstV1I	GCAGC	5 cut(s)	699, 760, 1396, 1448, 1612
BstX2I	RGATCY	1 cut(s)	345
BstXI	CCANNNNNNTGG	1 cut(s)	1051
BstYI	RGATCY	1 cut(s)	345
Bsu36I	CCTNAGG	1 cut(s)	603
BsuI	GTATCC	1 cut(s)	961
BsuRI	GGCC	5 cut(s)	359, 365, 875, 1033, 1358
BtgI	CCRYGG	1 cut(s)	315
BtgZI	GCGATG	1 cut(s)	862
BtsCI	GGATG	5 cut(s)	54, 60, 1097, 1390, 1435
BtsIMutI	CAGTG	1 cut(s)	1488
BveI	ACCTGC	1 cut(s)	643
Cac8I	GCNNGC	5 cut(s)	187, 397, 599, 710, 1623
CaiI	CAGNNNCTG	1 cut(s)	773
CciI	TCATGA	1 cut(s)	1200
CfoI	GCGC	2 cut(s)	143, 305
Cfr10I	RCCGGY	2 cut(s)	36, 1268
Cfr13I	GGNCC	3 cut(s)	39, 1031, 1357
Cfr42I	CCGCGG	1 cut(s)	318
CseI	GACGC	2 cut(s)	186, 454
CsiI	ACCWGGT	1 cut(s)	1084
Csp6I	GTAC	2 cut(s)	832, 904
CspAI	ACCGGT	2 cut(s)	36, 1268
CspCI	CAANNNNNGTGG	2 cut(s)	1069, 1104
CviAII	CATG	4 cut(s)	241, 532, 1045, 1201
CviQI	GTAC	2 cut(s)	832, 904
DdeI	CTNAG	7 cut(s)	120, 391, 603, 648, 761, 1482, 1491
DinI	GGCGCC	1 cut(s)	142
DrdI	GACNNNNNNGTC	1 cut(s)	563
DriI	GACNNNNNGTC	1 cut(s)	424
DseDI	GACNNNNNNGTC	1 cut(s)	563
EaeI	YGGCCR	1 cut(s)	873
Eam1104I	CTCTTC	1 cut(s)	1310
Eam1105I	GACNNNNNGTC	1 cut(s)	424
EarI	CTCTTC	1 cut(s)	1310
EciI	GGCGGA	1 cut(s)	465
Ecl136II	GAGCTC	3 cut(s)	117, 1120, 1303
Eco130I	CCWWGG	2 cut(s)	360, 949
Eco147I	AGGCCT	1 cut(s)	365
Eco24I	GRGCYC	3 cut(s)	119, 1122, 1305
Eco47I	GGWCC	1 cut(s)	39
Eco53kI	GAGCTC	3 cut(s)	117, 1120, 1303
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	1 cut(s)	112
Eco91I	GGTNACC	1 cut(s)	1190
EcoICRI	GAGCTC	3 cut(s)	117, 1120, 1303
EcoNI	CCTNNNNNAGG	1 cut(s)	953
EcoO65I	GGTNACC	1 cut(s)	1190
EcoRII	CCWGG	2 cut(s)	1084, 1168
EcoT14I	CCWWGG	2 cut(s)	360, 949
EcoT38I	GRGCYC	3 cut(s)	119, 1122, 1305
EgeI	GGCGCC	1 cut(s)	142
EheI	GGCGCC	1 cut(s)	142
ErhI	CCWWGG	2 cut(s)	360, 949
FaeI	CATG	4 cut(s)	244, 535, 1048, 1204
FalI	AAGNNNNNCTT	4 cut(s)	1055, 1087, 1197, 1229
FaqI	GGGAC	2 cut(s)	601, 1403
FatI	CATG	4 cut(s)	240, 531, 1044, 1200
FauI	CCCGC	1 cut(s)	346
FbaI	TGATCA	1 cut(s)	1332
FblI	GTMKAC	2 cut(s)	468, 1229
Fnu4HI	GCNGC	8 cut(s)	301, 318, 713, 774, 860, 1410, 1437, 1626
FokI	GGATG	5 cut(s)	41, 47, 1104, 1377, 1442
FriOI	GRGCYC	3 cut(s)	119, 1122, 1305
Fsp4HI	GCNGC	8 cut(s)	301, 318, 713, 774, 860, 1410, 1437, 1626
FspBI	CTAG	1 cut(s)	1395
GlaI	GCGC	2 cut(s)	142, 304
GluI	GCNGC	8 cut(s)	301, 318, 713, 774, 860, 1410, 1437, 1626
GsaI	CCCAGC	2 cut(s)	266, 1632
GsuI	CTGGAG	2 cut(s)	1191, 1461
HaeII	RGCGCY	2 cut(s)	144, 306
HaeIII	GGCC	5 cut(s)	359, 365, 875, 1033, 1358
HapII	CCGG	7 cut(s)	37, 147, 536, 585, 1223, 1269, 1451
HgaI	GACGC	2 cut(s)	186, 454
HhaI	GCGC	2 cut(s)	143, 305
Hin1I	GRCGYC	4 cut(s)	141, 234, 561, 696
Hin1II	CATG	4 cut(s)	244, 535, 1048, 1204
Hin6I	GCGC	2 cut(s)	141, 303
HinP1I	GCGC	2 cut(s)	141, 303
HincII	GTYRAC	3 cut(s)	469, 544, 1230
HindII	GTYRAC	3 cut(s)	469, 544, 1230
HindIII	AAGCTT	2 cut(s)	212, 1619
HinfI	GANTC	7 cut(s)	192, 204, 219, 470, 919, 1139, 1226
HpaII	CCGG	7 cut(s)	37, 147, 536, 585, 1223, 1269, 1451
HphI	GGTGA	6 cut(s)	379, 622, 1202, 1283, 1378, 1572
Hpy166II	GTNNAC	7 cut(s)	34, 445, 469, 544, 658, 758, 1230
Hpy188I	TCNGA	6 cut(s)	287, 486, 505, 1109, 1405, 1575
Hpy188III	TCNNGA	8 cut(s)	201, 446, 890, 896, 1123, 1136, 1201, 1223
Hpy8I	GTNNAC	7 cut(s)	34, 445, 469, 544, 658, 758, 1230
HpyAV	CCTTC	8 cut(s)	172, 289, 949, 1081, 1247, 1318, 1420, 1597
HpyCH4III	ACNGT	3 cut(s)	973, 1102, 1584
HpyCH4IV	ACGT	3 cut(s)	234, 561, 696
HpyCH4V	TGCA	5 cut(s)	496, 848, 1115, 1182, 1457
HpyF10VI	GCNNNNNNNGC	3 cut(s)	371, 773, 1622
HpyF3I	CTNAG	7 cut(s)	120, 391, 603, 648, 761, 1482, 1491
HpySE526I	ACGT	3 cut(s)	234, 561, 696
Hsp92I	GRCGYC	4 cut(s)	141, 234, 561, 696
Hsp92II	CATG	4 cut(s)	244, 535, 1048, 1204
HspAI	GCGC	2 cut(s)	141, 303
KasI	GGCGCC	1 cut(s)	140
Kpn2I	TCCGGA	1 cut(s)	1222
Ksp22I	TGATCA	1 cut(s)	1332
KspI	CCGCGG	1 cut(s)	318
LguI	GCTCTTC	1 cut(s)	1310
LmnI	GCTCC	4 cut(s)	255, 287, 770, 958
Lsp1109I	GCAGC	5 cut(s)	699, 760, 1396, 1448, 1612
LweI	GCATC	2 cut(s)	603, 1396
MabI	ACCWGGT	1 cut(s)	1084
MaeI	CTAG	1 cut(s)	1395
MaeII	ACGT	3 cut(s)	234, 561, 696
MaeIII	GTNAC	5 cut(s)	434, 634, 994, 1190, 1578
MboII	GAAGA	3 cut(s)	322, 503, 1297
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	4 cut(s)	119, 946, 1122, 1305
MlsI	TGGCCA	1 cut(s)	875
MluCI	AATT	2 cut(s)	1477, 1508
MluNI	TGGCCA	1 cut(s)	875
Mly113I	GGCGCC	1 cut(s)	141
MlyI	GAGTC	4 cut(s)	198, 201, 464, 1235
MmeI	TCCRAC	1 cut(s)	247
Mox20I	TGGCCA	1 cut(s)	875
MroI	TCCGGA	1 cut(s)	1222
MroXI	GAANNNNTTC	2 cut(s)	326, 1569
MscI	TGGCCA	1 cut(s)	875
MseI	TTAA	5 cut(s)	180, 350, 453, 930, 1215
MslI	CAYNNNNRTG	3 cut(s)	782, 1269, 1395
Msp20I	TGGCCA	1 cut(s)	875
MspA1I	CMGCKG	2 cut(s)	317, 1439
MspCI	CTTAAG	1 cut(s)	929
MspI	CCGG	7 cut(s)	37, 147, 536, 585, 1223, 1269, 1451
MspR9I	CCNGG	3 cut(s)	586, 1086, 1170
MvaI	CCWGG	2 cut(s)	1086, 1170
MvnI	CGCG	3 cut(s)	197, 300, 317
MwoI	GCNNNNNNNGC	3 cut(s)	371, 773, 1622
NarI	GGCGCC	1 cut(s)	141
NciI	CCSGG	1 cut(s)	586
NlaIII	CATG	4 cut(s)	244, 535, 1048, 1204
NlaIV	GGNNCC	7 cut(s)	142, 289, 945, 1099, 1349, 1359, 1449
NmeAIII	GCCGAG	1 cut(s)	367
NmuCI	GTSAC	5 cut(s)	434, 634, 994, 1190, 1578
NspI	RCATGY	1 cut(s)	535
OliI	CACNNNNGTG	1 cut(s)	1269
PaeR7I	CTCGAG	1 cut(s)	112
PagI	TCATGA	1 cut(s)	1200
PaqCI	CACCTGC	1 cut(s)	643
PceI	AGGCCT	1 cut(s)	365
PciSI	GCTCTTC	1 cut(s)	1310
PcsI	WCGNNNNNNNCGW	2 cut(s)	444, 587
PdmI	GAANNNNTTC	2 cut(s)	326, 1569
PfeI	GAWTC	3 cut(s)	219, 919, 1139
PflFI	GACNNNGTC	6 cut(s)	410, 560, 563, 695, 1045, 1499
PfoI	TCCNGGA	1 cut(s)	584
PinAI	ACCGGT	2 cut(s)	36, 1268
PkrI	GCNGC	8 cut(s)	302, 319, 714, 775, 861, 1411, 1438, 1627
Ple19I	CGATCG	2 cut(s)	855, 867
PleI	GAGTC	4 cut(s)	198, 200, 464, 1234
PluTI	GGCGCC	1 cut(s)	144
PpsI	GAGTC	4 cut(s)	198, 200, 464, 1234
PsiI	TTATAA	1 cut(s)	1536
Psp124BI	GAGCTC	3 cut(s)	119, 1122, 1305
Psp6I	CCWGG	2 cut(s)	1084, 1168
PspEI	GGTNACC	1 cut(s)	1190
PspFI	CCCAGC	2 cut(s)	262, 1628
PspGI	CCWGG	2 cut(s)	1084, 1168
PspN4I	GGNNCC	7 cut(s)	142, 289, 945, 1099, 1349, 1359, 1449
PspPI	GGNCC	3 cut(s)	39, 1031, 1357
PspXI	VCTCGAGB	1 cut(s)	112
PstNI	CAGNNNCTG	1 cut(s)	773
PsuI	RGATCY	1 cut(s)	345
PsyI	GACNNNGTC	6 cut(s)	410, 560, 563, 695, 1045, 1499
PvuI	CGATCG	2 cut(s)	855, 867
PvuII	CAGCTG	1 cut(s)	1439
RsaI	GTAC	2 cut(s)	833, 905
RsaNI	GTAC	2 cut(s)	832, 904
RseI	CAYNNNNRTG	3 cut(s)	782, 1269, 1395
SacI	GAGCTC	3 cut(s)	119, 1122, 1305
SacII	CCGCGG	1 cut(s)	318
SalI	GTCGAC	2 cut(s)	467, 1228
SapI	GCTCTTC	1 cut(s)	1310
SaqAI	TTAA	5 cut(s)	180, 350, 453, 930, 1215
SatI	GCNGC	8 cut(s)	301, 318, 713, 774, 860, 1410, 1437, 1626
Sau96I	GGNCC	3 cut(s)	39, 1031, 1357
SchI	GAGTC	4 cut(s)	198, 201, 464, 1235
ScrFI	CCNGG	3 cut(s)	586, 1086, 1170
SduI	GDGCHC	4 cut(s)	119, 946, 1122, 1305
SexAI	ACCWGGT	1 cut(s)	1084
SfaNI	GCATC	2 cut(s)	603, 1396
SfcI	CTRYAG	1 cut(s)	6
SfoI	GGCGCC	1 cut(s)	142
Sfr274I	CTCGAG	1 cut(s)	112
Sfr303I	CCGCGG	1 cut(s)	318
SgrAI	CRCCGGYG	1 cut(s)	1268
SgrBI	CCGCGG	1 cut(s)	318
SinI	GGWCC	1 cut(s)	39
SlaI	CTCGAG	1 cut(s)	112
SmiMI	CAYNNNNRTG	3 cut(s)	782, 1269, 1395
SmlI	CTYRAG	5 cut(s)	112, 929, 962, 1121, 1609
SmoI	CTYRAG	5 cut(s)	112, 929, 962, 1121, 1609
Sse9I	AATT	2 cut(s)	1477, 1508
SseBI	AGGCCT	1 cut(s)	365
SsiI	CCGC	7 cut(s)	300, 315, 317, 339, 476, 860, 1600
SspDI	GGCGCC	1 cut(s)	140
SspMI	CTAG	1 cut(s)	1395
SstI	GAGCTC	3 cut(s)	119, 1122, 1305
StuI	AGGCCT	1 cut(s)	365
StyD4I	CCNGG	3 cut(s)	584, 1084, 1168
StyI	CCWWGG	2 cut(s)	360, 949
TaaI	ACNGT	3 cut(s)	973, 1102, 1584
TaiI	ACGT	3 cut(s)	237, 564, 699
TasI	AATT	2 cut(s)	1477, 1508
TatI	WGTACW	1 cut(s)	831
TauI	GCSGC	3 cut(s)	303, 320, 862
TfiI	GAWTC	3 cut(s)	219, 919, 1139
Tru1I	TTAA	5 cut(s)	180, 350, 453, 930, 1215
Tru9I	TTAA	5 cut(s)	180, 350, 453, 930, 1215
TscAI	CASTG	1 cut(s)	1495
TseFI	GTSAC	5 cut(s)	434, 634, 994, 1190, 1578
TseI	GCWGC	5 cut(s)	712, 773, 1409, 1436, 1625
Tsp45I	GTSAC	5 cut(s)	434, 634, 994, 1190, 1578
TspDTI	ATGAA	8 cut(s)	537, 713, 803, 1217, 1273, 1297, 1439, 1549
TspRI	CASTG	1 cut(s)	1495
Tth111I	GACNNNGTC	6 cut(s)	410, 560, 563, 695, 1045, 1499
Vha464I	CTTAAG	1 cut(s)	929
VpaK11BI	GGWCC	1 cut(s)	39
XagI	CCTNNNNNAGG	1 cut(s)	953
XceI	RCATGY	1 cut(s)	535
XhoI	CTCGAG	1 cut(s)	112
XmiI	GTMKAC	2 cut(s)	468, 1229
XmnI	GAANNNNTTC	2 cut(s)	326, 1569
XspI	CTAG	1 cut(s)	1395
ZraI	GACGTC	3 cut(s)	235, 562, 697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.