Rh2DG375300

4-coumarate-coa ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001962
Physical Location & Seq
Reverse (-)
53545695 .. 53550792
5098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG375300.1

Sequence Viewer

Length: 1413 bp
ATGGCAATCGAAACACCCCAAAGCGACATCATCTACCGATCCAAGCTCCCGGACATTCCCATTCCAAACCACCTCCCTCTCCATTCCTACATCTTCCAAAACAAATCCCACCTCAGCTCAAAGCCTTGCATCATCAATGGCACCACCGGCGAGATCCACACCTACGCCGATGTAGAGCTCACCGCCCGGAAAGTCGCCTCCGGTCTCAACAAGCTCGGCATCAAGCAAGGCGACGTCGTCATGCTCCTCCTCCCCAACACTCCCGAATTCGTCTTCACCTTCTTGGGAGCCTCCTTCCGCGGAGCCATGACCACCGCCGCCAACCCTTTCTTCACTCCAGCGGAGATAGCCAAACAAGCGAAAGCATCGAAAGCCAAGCTGATCATCACTCTCGCTATCTATTACGACAAAGTTAAAGAGTTGTCATGCGACGAAGTCAAGTTGATGTGCATTGACTCCCCGCCACCCGACTCGTCTTGTCTCCATTTCTCCGAACTGACTCAGTCCAATGAGGACGACGTGCCGGAAGTTGACATCAGCCCGAACGACGTCGTGTTGTTACCCTACTCGTCCGGCACGACGGGGCTGCCGAAAGGGGTGATGCTGACGCACAAGGGCTTGGTAACGAGCGTGTCTCAGCAGGTTGACGGAGAGAATCCGAACTTGTACTACAACAGCGACGACGTCGTTCTGTGTGTGCTGCCGCTGTTTCACATTTACTCGCTGAACTCGGTCTTGCTATGTGGGTTGAGAGCCGGAGCTGCCATTCTGGTGATGCAGAAGTTTGAAATTGTTTCTCTTTTGGAGCTCATGCAGAAGCATAGAGTTAGTGTTGCGCCGGTCGCGCCTCCGATTGTTCTGGCGATCGCGAAGTTTCCCGATCTCGACAAGTACGATTTGGGGTCGATAAGGGTGCTGAAGAGTGGAGGAGCACCGTTGGGGAAGGAGCTTGAAGACACAGTCAGAGCGAAATTTCCCAATGTAACGCTTGGTCAGGGGTATGGGATGACGGAGGCAGGACCGGTTTTGACAATGTCATTGGCATTTGCAAAAGAACCCTTTGAGGTAAAACCAGGTGGGTGCGGAACTGTGGTCAGAAATGCAGAAATGAAGATCGTAGACCCTGAAACTGGTGCCTCTTTGCCGCGCAACCAGCCTGGAGAGATTTGCATCAGAGGTCACCAGATCATGAAAGGTTACCTTAATGATCCGGAGGCCACAAGCACAACGATAGACAAGGAAGGATGGCTACACACAGGTGACATAGGCTTCATTGATGATGATGAAGAGCTCTTCATTGTTGATCGACTGAAGGAGATTATCAAATACAAAGGATTTCAGGTGGCTCCTGCTGAGCTTGAAGCCTTGCTCGTCACCCATCCTAACATTTCTGATGCCGCAGTTGTCTCGTAA

Protein Analysis

470

Amino Acids

51.0

Weight (kDa)

5.23

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 40 - 401 8.9e-97 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 237, 552, 687
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 2 cut(s) 140, 1133
AccI GTMKAC 1 cut(s) 1119
AccII CGCG 4 cut(s) 300, 845, 869, 1147
AccIII TCCGGA 1 cut(s) 1210
AclWI GGATC 3 cut(s) 33, 148, 1202
AcsI RAATTY 2 cut(s) 266, 971
AcuI CTGAAG 2 cut(s) 938, 1331
AcyI GRCGYC 3 cut(s) 234, 549, 684
AfaI GTAC 2 cut(s) 668, 893
AfiI CCNNNNNNNGG 1 cut(s) 1130
AgeI ACCGGT 1 cut(s) 1021
AgsI TTSAA 3 cut(s) 788, 953, 1361
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 2 cut(s) 1072, 1156
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AleI CACNNNNGTG 1 cut(s) 1257
Alw21I GWGCWC 4 cut(s) 180, 810, 934, 1293
Alw26I GTCTC 3 cut(s) 209, 485, 639
AlwI GGATC 3 cut(s) 33, 148, 1202
Aor13HI TCCGGA 1 cut(s) 1210
AoxI GGCC 1 cut(s) 1215
ApeKI GCWGC 3 cut(s) 586, 700, 761
ApoI RAATTY 2 cut(s) 266, 971
AsiGI ACCGGT 1 cut(s) 1021
AsiSI GCGATCGC 1 cut(s) 867
AspLEI GCGC 3 cut(s) 838, 847, 1149
AspS9I GGNCC 1 cut(s) 1019
AsuC2I CCSGG 2 cut(s) 50, 187
AsuHPI GGTGA 7 cut(s) 172, 268, 610, 784, 1172, 1271, 1366
AvaII GGWCC 1 cut(s) 1019
BanI GGYRCC 2 cut(s) 140, 1133
BanII GRGCYC 3 cut(s) 180, 810, 1293
BarI GAAGNNNNNNTAC 2 cut(s) 1233, 1265
BbsI GAAGAC 2 cut(s) 265, 960
Bbv12I GWGCWC 4 cut(s) 180, 810, 934, 1293
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 573, 687, 748
BccI CCATC 2 cut(s) 1239, 1386
BcgI CGANNNNNNTGC 5 cut(s) 568, 602, 895, 929, 1389
BciT130I CCWGG 2 cut(s) 1074, 1158
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 2 cut(s) 50, 187
BcoDI GTCTC 3 cut(s) 209, 485, 639
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 7 cut(s) 318, 587, 701, 704, 762, 1145, 1398
BlpI GCTNAGC 1 cut(s) 1353
BlsI GCNGC 7 cut(s) 319, 588, 702, 705, 763, 1146, 1399
Bme1390I CCNGG 4 cut(s) 50, 187, 1074, 1158
Bme18I GGWCC 1 cut(s) 1019
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 1 cut(s) 1019
BmiI GGNNCC 5 cut(s) 142, 289, 304, 1135, 1347
BmrFI CCNGG 4 cut(s) 50, 187, 1074, 1158
BmsI GCATC 7 cut(s) 138, 228, 374, 591, 765, 1179, 1384
BpiI GAAGAC 2 cut(s) 265, 960
BplI GAGNNNNNCTC 2 cut(s) 619, 651
BpmI CTGGAG 2 cut(s) 321, 1179
Bpu10I CCTNAGC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 1353
BpuMI CCSGG 2 cut(s) 50, 187
BsaBI GATNNNNATC 1 cut(s) 1169
BsaHI GRCGYC 3 cut(s) 234, 549, 684
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 298
BsaWI WCCGGW 3 cut(s) 200, 1021, 1210
Bsc4I CCNNNNNNNGG 1 cut(s) 1130
Bse118I RCCGGY 3 cut(s) 146, 838, 1021
Bse1I ACTGG 1 cut(s) 1135
Bse8I GATNNNNATC 1 cut(s) 1169
BseAI TCCGGA 1 cut(s) 1210
BseBI CCWGG 2 cut(s) 1074, 1158
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 3 cut(s) 1011, 1250, 1378
BseJI GATNNNNATC 1 cut(s) 1169
BseLI CCNNNNNNNGG 1 cut(s) 1130
BseMII CTCAG 4 cut(s) 127, 515, 650, 1344
BseNI ACTGG 1 cut(s) 1135
BseRI GAGGAG 3 cut(s) 236, 239, 942
BseXI GCAGC 3 cut(s) 573, 687, 748
Bsh1236I CGCG 4 cut(s) 300, 845, 869, 1147
Bsh1285I CGRYCG 2 cut(s) 843, 867
BshFI GGCC 1 cut(s) 1217
BshNI GGYRCC 2 cut(s) 140, 1133
BshTI ACCGGT 1 cut(s) 1021
BsiEI CGRYCG 2 cut(s) 843, 867
BsiHKAI GWGCWC 4 cut(s) 180, 810, 934, 1293
BslI CCNNNNNNNGG 1 cut(s) 1130
BsmAI GTCTC 3 cut(s) 209, 485, 639
BsnI GGCC 1 cut(s) 1217
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 4 cut(s) 180, 810, 934, 1293
Bsp13I TCCGGA 1 cut(s) 1210
Bsp143I GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
Bsp1720I GCTNAGC 1 cut(s) 1353
Bsp68I TCGCGA 1 cut(s) 869
BspANI GGCC 1 cut(s) 1217
BspCNI CTCAG 4 cut(s) 126, 514, 649, 1345
BspEI TCCGGA 1 cut(s) 1210
BspFNI CGCG 4 cut(s) 300, 845, 869, 1147
BspHI TCATGA 1 cut(s) 1188
BspLI GGNNCC 5 cut(s) 142, 289, 304, 1135, 1347
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 3 cut(s) 33, 148, 1202
BspQI GCTCTTC 2 cut(s) 1281, 1298
BspT107I GGYRCC 2 cut(s) 140, 1133
BspTNI GGTCTC 1 cut(s) 209
BsrFI RCCGGY 3 cut(s) 146, 838, 1021
BsrI ACTGG 1 cut(s) 1135
BssAI RCCGGY 3 cut(s) 146, 838, 1021
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
BssNI GRCGYC 3 cut(s) 234, 549, 684
Bst2UI CCWGG 2 cut(s) 1074, 1158
Bst4CI ACNGT 3 cut(s) 936, 961, 1090
Bst6I CTCTTC 3 cut(s) 914, 1281, 1298
BstACI GRCGYC 3 cut(s) 234, 549, 684
BstDEI CTNAG 4 cut(s) 113, 501, 636, 1353
BstDSI CCRYGG 1 cut(s) 298
BstEII GGTNACC 2 cut(s) 1178, 1196
BstF5I GGATG 3 cut(s) 1011, 1250, 1378
BstFNI CGCG 4 cut(s) 300, 845, 869, 1147
BstHHI GCGC 3 cut(s) 838, 847, 1149
BstKTI GATC 9 cut(s) 41, 156, 384, 867, 883, 1116, 1188, 1210, 1306
BstMAI GTCTC 3 cut(s) 209, 485, 639
BstMBI GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
BstMCI CGRYCG 2 cut(s) 843, 867
BstMWI GCNNNNNNNGC 8 cut(s) 147, 347, 356, 371, 761, 842, 844, 1153
BstNI CCWGG 2 cut(s) 1074, 1158
BstPI GGTNACC 2 cut(s) 1178, 1196
BstSCI CCNGG 4 cut(s) 48, 185, 1072, 1156
BstUI CGCG 4 cut(s) 300, 845, 869, 1147
BstV1I GCAGC 3 cut(s) 573, 687, 748
BstV2I GAAGAC 2 cut(s) 265, 960
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 1 cut(s) 1217
BtgI CCRYGG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 1011, 1250, 1378
BtuMI TCGCGA 1 cut(s) 869
BveI ACCTGC 1 cut(s) 631
CciI TCATGA 1 cut(s) 1188
CfoI GCGC 3 cut(s) 838, 847, 1149
Cfr10I RCCGGY 3 cut(s) 146, 838, 1021
Cfr13I GGNCC 1 cut(s) 1019
Cfr42I CCGCGG 1 cut(s) 301
CseI GACGC 1 cut(s) 616
CsiI ACCWGGT 1 cut(s) 1072
Csp6I GTAC 2 cut(s) 667, 892
CspAI ACCGGT 1 cut(s) 1021
CviAII CATG 5 cut(s) 241, 307, 426, 811, 1189
CviQI GTAC 2 cut(s) 667, 892
DdeI CTNAG 4 cut(s) 113, 501, 636, 1353
DpnI GATC 9 cut(s) 40, 155, 383, 866, 882, 1115, 1187, 1209, 1305
DpnII GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
Eam1104I CTCTTC 3 cut(s) 914, 1281, 1298
EarI CTCTTC 3 cut(s) 914, 1281, 1298
Ecl136II GAGCTC 3 cut(s) 178, 808, 1291
Eco24I GRGCYC 3 cut(s) 180, 810, 1293
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 1019
Eco53kI GAGCTC 3 cut(s) 178, 808, 1291
Eco57I CTGAAG 2 cut(s) 938, 1331
Eco91I GGTNACC 2 cut(s) 1178, 1196
EcoICRI GAGCTC 3 cut(s) 178, 808, 1291
EcoO65I GGTNACC 2 cut(s) 1178, 1196
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 1072, 1156
EcoT38I GRGCYC 3 cut(s) 180, 810, 1293
FaeI CATG 5 cut(s) 244, 310, 429, 814, 1192
FaiI YATR 9 cut(s) 242, 308, 427, 742, 812, 822, 1002, 1190, 1265
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FatI CATG 5 cut(s) 240, 306, 425, 810, 1188
FauI CCCGC 1 cut(s) 468
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 1 cut(s) 1119
Fnu4HI GCNGC 7 cut(s) 318, 587, 701, 704, 762, 1145, 1398
FokI GGATG 3 cut(s) 1018, 1257, 1365
FriOI GRGCYC 3 cut(s) 180, 810, 1293
Fsp4HI GCNGC 7 cut(s) 318, 587, 701, 704, 762, 1145, 1398
GlaI GCGC 3 cut(s) 837, 846, 1148
GluI GCNGC 7 cut(s) 318, 587, 701, 704, 762, 1145, 1398
GsuI CTGGAG 2 cut(s) 321, 1179
HaeIII GGCC 1 cut(s) 1217
HgaI GACGC 1 cut(s) 616
HhaI GCGC 3 cut(s) 838, 847, 1149
Hin1I GRCGYC 3 cut(s) 234, 549, 684
Hin1II CATG 5 cut(s) 244, 310, 429, 814, 1192
Hin6I GCGC 3 cut(s) 836, 845, 1147
HinP1I GCGC 3 cut(s) 836, 845, 1147
HincII GTYRAC 2 cut(s) 532, 646
HindII GTYRAC 2 cut(s) 532, 646
HinfI GANTC 4 cut(s) 455, 470, 499, 655
HphI GGTGA 7 cut(s) 172, 268, 610, 784, 1172, 1271, 1366
Hpy166II GTNNAC 3 cut(s) 532, 646, 1120
Hpy188I TCNGA 7 cut(s) 493, 660, 852, 965, 1097, 1175, 1393
Hpy188III TCNNGA 6 cut(s) 263, 868, 878, 884, 1189, 1211
Hpy8I GTNNAC 3 cut(s) 532, 646, 1120
HpyAV CCTTC 5 cut(s) 289, 304, 937, 1235, 1306
HpyCH4III ACNGT 3 cut(s) 936, 961, 1090
HpyCH4IV ACGT 4 cut(s) 234, 519, 549, 684
HpyCH4V TGCA 7 cut(s) 129, 450, 778, 814, 1049, 1103, 1170
HpyF10VI GCNNNNNNNGC 8 cut(s) 147, 347, 356, 371, 761, 842, 844, 1153
HpyF3I CTNAG 4 cut(s) 113, 501, 636, 1353
HpySE526I ACGT 4 cut(s) 234, 519, 549, 684
Hsp92I GRCGYC 3 cut(s) 234, 549, 684
Hsp92II CATG 5 cut(s) 244, 310, 429, 814, 1192
HspAI GCGC 3 cut(s) 836, 845, 1147
Kpn2I TCCGGA 1 cut(s) 1210
Ksp22I TGATCA 1 cut(s) 381
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
LguI GCTCTTC 2 cut(s) 1281, 1298
LmnI GCTCC 9 cut(s) 51, 249, 287, 302, 758, 805, 929, 946, 1351
Lsp1109I GCAGC 3 cut(s) 573, 687, 748
LweI GCATC 7 cut(s) 138, 228, 374, 591, 765, 1179, 1384
MabI ACCWGGT 1 cut(s) 1072
MaeII ACGT 4 cut(s) 234, 519, 549, 684
MaeIII GTNAC 7 cut(s) 558, 622, 982, 1178, 1196, 1259, 1372
MalI GATC 9 cut(s) 40, 155, 383, 866, 882, 1115, 1187, 1209, 1305
MboI GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
MboII GAAGA 8 cut(s) 85, 265, 322, 931, 965, 1123, 1285, 1298
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 4 cut(s) 180, 810, 934, 1293
MluCI AATT 3 cut(s) 266, 789, 971
MlyI GAGTC 3 cut(s) 449, 464, 493
MroI TCCGGA 1 cut(s) 1210
MseI TTAA 2 cut(s) 414, 1203
MslI CAYNNNNRTG 2 cut(s) 770, 1257
MspA1I CMGCKG 3 cut(s) 300, 341, 706
MspR9I CCNGG 4 cut(s) 50, 187, 1074, 1158
MvaI CCWGG 2 cut(s) 1074, 1158
MvnI CGCG 4 cut(s) 300, 845, 869, 1147
MwoI GCNNNNNNNGC 8 cut(s) 147, 347, 356, 371, 761, 842, 844, 1153
NciI CCSGG 2 cut(s) 50, 187
NdeII GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
NlaIII CATG 5 cut(s) 244, 310, 429, 814, 1192
NlaIV GGNNCC 5 cut(s) 142, 289, 304, 1135, 1347
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 3 cut(s) 1178, 1259, 1372
NruI TCGCGA 1 cut(s) 869
OliI CACNNNNGTG 1 cut(s) 1257
PagI TCATGA 1 cut(s) 1188
PciSI GCTCTTC 2 cut(s) 1281, 1298
PcsI WCGNNNNNNNCGW 3 cut(s) 575, 587, 891
PfeI GAWTC 1 cut(s) 655
PflFI GACNNNGTC 6 cut(s) 236, 434, 502, 683, 959, 1033
PfoI TCCNGGA 1 cut(s) 48
PinAI ACCGGT 1 cut(s) 1021
PkrI GCNGC 7 cut(s) 319, 588, 702, 705, 763, 1146, 1399
Ple19I CGATCG 1 cut(s) 867
PleI GAGTC 3 cut(s) 449, 464, 493
PpsI GAGTC 3 cut(s) 449, 464, 493
Psp124BI GAGCTC 3 cut(s) 180, 810, 1293
Psp6I CCWGG 2 cut(s) 1072, 1156
PspEI GGTNACC 2 cut(s) 1178, 1196
PspGI CCWGG 2 cut(s) 1072, 1156
PspN4I GGNNCC 5 cut(s) 142, 289, 304, 1135, 1347
PspPI GGNCC 1 cut(s) 1019
PsrI GAACNNNNNNTAC 2 cut(s) 653, 685
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 6 cut(s) 236, 434, 502, 683, 959, 1033
PvuI CGATCG 1 cut(s) 867
RgaI GCGATCGC 1 cut(s) 867
RruI TCGCGA 1 cut(s) 869
RsaI GTAC 2 cut(s) 668, 893
RsaNI GTAC 2 cut(s) 667, 892
RseI CAYNNNNRTG 2 cut(s) 770, 1257
SacI GAGCTC 3 cut(s) 180, 810, 1293
SacII CCGCGG 1 cut(s) 301
SapI GCTCTTC 2 cut(s) 1281, 1298
SaqAI TTAA 2 cut(s) 414, 1203
SatI GCNGC 7 cut(s) 318, 587, 701, 704, 762, 1145, 1398
Sau3AI GATC 9 cut(s) 38, 153, 381, 864, 880, 1113, 1185, 1207, 1303
Sau96I GGNCC 1 cut(s) 1019
SchI GAGTC 3 cut(s) 449, 464, 493
ScrFI CCNGG 4 cut(s) 50, 187, 1074, 1158
SduI GDGCHC 4 cut(s) 180, 810, 934, 1293
SexAI ACCWGGT 1 cut(s) 1072
SfaAI GCGATCGC 1 cut(s) 867
SfaNI GCATC 7 cut(s) 138, 228, 374, 591, 765, 1179, 1384
Sfr303I CCGCGG 1 cut(s) 301
SgfI GCGATCGC 1 cut(s) 867
SgrAI CRCCGGYG 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 301
SinI GGWCC 1 cut(s) 1019
SmiMI CAYNNNNRTG 2 cut(s) 770, 1257
Sse9I AATT 3 cut(s) 266, 789, 971
SstI GAGCTC 3 cut(s) 180, 810, 1293
StyD4I CCNGG 4 cut(s) 48, 185, 1072, 1156
TaaI ACNGT 3 cut(s) 936, 961, 1090
TaiI ACGT 4 cut(s) 237, 522, 552, 687
TaqI TCGA 5 cut(s) 9, 368, 885, 905, 1306
TaqII GACCGA 1 cut(s) 721
TasI AATT 3 cut(s) 266, 789, 971
TatI WGTACW 1 cut(s) 666
TauI GCSGC 4 cut(s) 320, 706, 1147, 1400
TfiI GAWTC 1 cut(s) 655
Tru1I TTAA 2 cut(s) 414, 1203
Tru9I TTAA 2 cut(s) 414, 1203
TseFI GTSAC 3 cut(s) 1178, 1259, 1372
TseI GCWGC 3 cut(s) 586, 700, 761
Tsp45I GTSAC 3 cut(s) 1178, 1259, 1372
TspDTI ATGAA 5 cut(s) 1124, 1205, 1261, 1285, 1299
TspGWI ACGGA 2 cut(s) 663, 1025
Tth111I GACNNNGTC 6 cut(s) 236, 434, 502, 683, 959, 1033
VpaK11BI GGWCC 1 cut(s) 1019
XapI RAATTY 2 cut(s) 266, 971
XmiI GTMKAC 1 cut(s) 1119
ZraI GACGTC 3 cut(s) 235, 550, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.