Rh4DG108000

4-coumarate-coa ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001962
Physical Location & Seq
Forward (+)
16566836 .. 16572054
5219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG108000.1

Sequence Viewer

Length: 1353 bp
ATGTCACCATCACACCTACTTGTATATTACATCAACCAAAACTCAAACCAAAACAGGGAGAGACTTCACCGCAAGAAAGCTAAACCCAACCCAATCCCCACCTTCTCCTTTTCTCTCTCACATTTTCCGTTCGCACTTTCCAAAATGGAGAACAAACAAGACAGTCACGAATTCATTTTCCGTTCAAAGCTGCCGGACATTTACATCCCGAACCACCTCCCCCTCCACACCTACTGCTTCGAGAACATCTCCCAATTCCATGACCGTCCCTGCCTGATCAACGGCAACACCGGCGAGACCTTCACCTATGCCGACGTCGAGCTCACCTCCCGCAGAGTCGCCGCCGGTCTCGACAAGCTCGGCATTCACCAGGGCGACGTCGTCATGCTCCTCCTCCAAAACTGCCCTGAATTCGCCTTCGCATTCCTCGGCGCCTCTTACGTGGGAGGCATCATCACTACGGCGAACCCCTTCTACACTCCTGCCGAGGTCGCCAAGCAAGCCACTGCCTCCAACGCCAAGCTGATCATAACTCAAGCGGCCTACGTGGACAAGGTGAAGGACTTTGCCAAACTCAACAACGTTAAACTCATGTGCGTGGATGAGACTTCGTCGTCATCACAGGATGTGTTGCATTTCTCCCAGCTGACGTCGGCTGACGAGAGCGAAATCCCGGCGGTGAAAATCAACCCGGACGACGTGGTGGCGCTGCCGTACTCGTCGGGGACGACGGGGCTGCCGAAAGGGGTGATGCTGTCGCACAAGGGGTTGGTGACGAGCGTGGCGCAGCAGGTAGACGGCGAAAACCCAAACCTCTACTTTCACAAGGAGGACGTGATCCTCTGCGTGCTGCCGTTGTTCCACATCTACTCCCTCAACTCGGTGTTCCTCTGCGGACTCCGCGTCGGAGCGACAATCCTGATCATGCAGAAGTTTGAGATCAACAAGTTGTTGGAGTTGGTGGAAAAGGAAAAGGTGACTATTGCGCCGTTTGTGCCTCCGATTGTTTTGAACATCGCCAAGAGTCCGGACCTGCACCGGTACGACTTGTCGTCGATTCGGATGGTGATGTCCGGGGCGGCTCCGATGGGGAAGGAGCTCGAGGACACTGTCAGGGCTAAACTGCCCAATGCTAAACTCGGACAGGGTTATGGGATGACCGAGGCTGGACCAGTTCTATCCATGTGCTTGGCATTTGCAAAAGAACCCTATGATATAAAATCAGGTGCGTGCGGGACCGTCGTAAGAAATGCTGAGATGAAGATCATCGACCCCGACACCAATGAATCCCTTCCCCGAAACCAATCAGGAGAGATTTGCATCCGAGGTAGCCAAATCATGAAAGGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

450

Amino Acids

49.6

Weight (kDa)

6.67

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 81 - 449 1.6e-99 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 613
AatII GACGTC 3 cut(s) 318, 381, 653
Acc36I ACCTGC 2 cut(s) 781, 1041
AccB1I GGYRCC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 795
AccII CGCG 1 cut(s) 903
AccIII TCCGGA 1 cut(s) 1027
AciI CCGC 9 cut(s) 70, 331, 342, 539, 677, 894, 901, 1079, 1233
AclI AACGTT 1 cut(s) 582
AclWI GGATC 1 cut(s) 832
AcsI RAATTY 2 cut(s) 170, 410
AcyI GRCGYC 4 cut(s) 315, 378, 432, 650
AfaI GTAC 3 cut(s) 716, 1043, 1348
AfiI CCNNNNNNNGG 2 cut(s) 55, 880
AgeI ACCGGT 1 cut(s) 1038
AgsI TTSAA 2 cut(s) 186, 1012
AhdI GACNNNNNGTC 2 cut(s) 902, 1051
AjiI CACGTC 2 cut(s) 700, 835
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 7 cut(s) 80, 190, 322, 358, 523, 646, 1099
AluI AGCT 7 cut(s) 80, 190, 322, 358, 523, 646, 1099
Alw21I GWGCWC 2 cut(s) 324, 1101
Alw26I GTCTC 4 cut(s) 55, 290, 353, 599
AlwI GGATC 1 cut(s) 832
Ama87I CYCGRG 1 cut(s) 1100
Aor13HI TCCGGA 1 cut(s) 1027
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 5 cut(s) 190, 709, 736, 787, 850
ApoI RAATTY 2 cut(s) 170, 410
AsiGI ACCGGT 1 cut(s) 1038
Asp700I GAANNNNTTC 2 cut(s) 470, 1290
AspLEI GCGC 4 cut(s) 434, 709, 787, 988
AspS9I GGNCC 3 cut(s) 1030, 1169, 1236
AsuC2I CCSGG 3 cut(s) 674, 692, 1075
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 3 cut(s) 1030, 1169, 1236
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 2 cut(s) 324, 1101
Bbv12I GWGCWC 2 cut(s) 324, 1101
BbvI GCAGC 5 cut(s) 177, 696, 723, 799, 837
BccI CCATC 3 cut(s) 16, 1057, 1081
BceAI ACGGC 6 cut(s) 298, 477, 697, 814, 838, 973
BcgI CGANNNNNNTGC 2 cut(s) 718, 752
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 3 cut(s) 276, 525, 921
BcnI CCSGG 3 cut(s) 674, 692, 1075
BcoDI GTCTC 4 cut(s) 55, 290, 353, 599
BfoI RGCGCY 2 cut(s) 435, 710
BfuAI ACCTGC 2 cut(s) 781, 1041
BisI GCNGC 8 cut(s) 191, 342, 540, 710, 737, 788, 851, 1080
BlsI GCNGC 8 cut(s) 192, 343, 541, 711, 738, 789, 852, 1081
Bme1390I CCNGG 4 cut(s) 371, 674, 692, 1075
Bme18I GGWCC 3 cut(s) 1030, 1169, 1236
BmeRI GACNNNNNGTC 2 cut(s) 902, 1051
BmeT110I CYCGRG 1 cut(s) 1100
BmgBI CACGTC 2 cut(s) 700, 835
BmgT120I GGNCC 3 cut(s) 1030, 1169, 1236
BmiI GGNNCC 3 cut(s) 433, 1083, 1237
BmrFI CCNGG 4 cut(s) 371, 674, 692, 1075
BmsI GCATC 3 cut(s) 459, 741, 1329
BplI GAGNNNNNCTC 4 cut(s) 233, 265, 311, 343
BpuEI CTTGAG 1 cut(s) 519
BpuMI CCSGG 3 cut(s) 674, 692, 1075
BsaAI YACGTR 3 cut(s) 442, 547, 1350
BsaBI GATNNNNATC 2 cut(s) 1262, 1319
BsaHI GRCGYC 4 cut(s) 315, 378, 432, 650
BsaI GGTCTC 2 cut(s) 290, 353
BsaJI CCNNGG 6 cut(s) 370, 427, 486, 1074, 1161, 1324
BsaWI WCCGGW 2 cut(s) 1027, 1038
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 880
Bse118I RCCGGY 3 cut(s) 290, 344, 1038
Bse1I ACTGG 1 cut(s) 1172
Bse8I GATNNNNATC 2 cut(s) 1262, 1319
BseAI TCCGGA 1 cut(s) 1027
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 6 cut(s) 370, 427, 486, 1074, 1161, 1324
BseGI GGATG 6 cut(s) 204, 607, 631, 1068, 1161, 1320
BseJI GATNNNNATC 2 cut(s) 1262, 1319
BseLI CCNNNNNNNGG 2 cut(s) 55, 880
BseMII CTCAG 1 cut(s) 1245
BseNI ACTGG 1 cut(s) 1172
BseRI GAGGAG 2 cut(s) 380, 383
BseXI GCAGC 5 cut(s) 177, 696, 723, 799, 837
BseYI CCCAGC 1 cut(s) 642
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 1 cut(s) 542
BshNI GGYRCC 1 cut(s) 431
BshTI ACCGGT 1 cut(s) 1038
BsiHKAI GWGCWC 2 cut(s) 324, 1101
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 8 cut(s) 194, 291, 345, 674, 692, 1028, 1039, 1074
BslFI GGGAC 3 cut(s) 252, 739, 1249
BslI CCNNNNNNNGG 2 cut(s) 55, 880
BsmAI GTCTC 4 cut(s) 55, 290, 353, 599
BsmFI GGGAC 3 cut(s) 252, 739, 1249
BsmI GAATGC 2 cut(s) 363, 422
BsnI GGCC 1 cut(s) 542
Bso31I GGTCTC 2 cut(s) 290, 353
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 2 cut(s) 324, 1101
Bsp13I TCCGGA 1 cut(s) 1027
Bsp143I GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
BspACI CCGC 9 cut(s) 70, 331, 342, 539, 677, 894, 901, 1079, 1233
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 1 cut(s) 1246
BspEI TCCGGA 1 cut(s) 1027
BspFNI CGCG 1 cut(s) 903
BspHI TCATGA 1 cut(s) 1338
BspLI GGNNCC 3 cut(s) 433, 1083, 1237
BspMI ACCTGC 2 cut(s) 781, 1041
BspPI GGATC 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 431
BspTNI GGTCTC 2 cut(s) 290, 353
BsrFI RCCGGY 3 cut(s) 290, 344, 1038
BsrI ACTGG 1 cut(s) 1172
BssAI RCCGGY 3 cut(s) 290, 344, 1038
BssECI CCNNGG 6 cut(s) 370, 427, 486, 1074, 1161, 1324
BssMI GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
BssNI GRCGYC 4 cut(s) 315, 378, 432, 650
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 4 cut(s) 164, 266, 1111, 1240
BstACI GRCGYC 4 cut(s) 315, 378, 432, 650
BstBAI YACGTR 3 cut(s) 442, 547, 1350
BstC8I GCNNGC 3 cut(s) 501, 848, 1231
BstDEI CTNAG 1 cut(s) 1254
BstF5I GGATG 6 cut(s) 204, 607, 631, 1068, 1161, 1320
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 2 cut(s) 435, 710
BstHHI GCGC 4 cut(s) 434, 709, 787, 988
BstKTI GATC 6 cut(s) 279, 528, 840, 924, 942, 1266
BstMAI GTCTC 4 cut(s) 55, 290, 353, 599
BstMBI GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
BstMWI GCNNNNNNNGC 6 cut(s) 291, 491, 500, 515, 900, 994
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 4 cut(s) 369, 672, 690, 1073
BstSNI TACGTA 1 cut(s) 1350
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 5 cut(s) 177, 696, 723, 799, 837
BstXI CCANNNNNNTGG 1 cut(s) 1189
BsuRI GGCC 1 cut(s) 542
BtgZI GCGATG 1 cut(s) 1000
BtrI CACGTC 2 cut(s) 700, 835
BtsCI GGATG 6 cut(s) 204, 607, 631, 1068, 1161, 1320
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 504, 1107
BveI ACCTGC 2 cut(s) 781, 1041
Cac8I GCNNGC 3 cut(s) 501, 848, 1231
CciI TCATGA 1 cut(s) 1338
CfoI GCGC 4 cut(s) 434, 709, 787, 988
Cfr10I RCCGGY 3 cut(s) 290, 344, 1038
Cfr13I GGNCC 3 cut(s) 1030, 1169, 1236
CseI GACGC 1 cut(s) 892
Csp6I GTAC 3 cut(s) 715, 1042, 1347
CspAI ACCGGT 1 cut(s) 1038
CviAII CATG 6 cut(s) 260, 385, 592, 925, 1183, 1339
CviQI GTAC 3 cut(s) 715, 1042, 1347
DdeI CTNAG 1 cut(s) 1254
DinI GGCGCC 1 cut(s) 433
DpnI GATC 6 cut(s) 278, 527, 839, 923, 941, 1265
DpnII GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
DrdI GACNNNNNNGTC 1 cut(s) 613
DriI GACNNNNNGTC 2 cut(s) 902, 1051
DseDI GACNNNNNNGTC 1 cut(s) 613
Eam1105I GACNNNNNGTC 2 cut(s) 902, 1051
Ecl136II GAGCTC 2 cut(s) 322, 1099
Eco105I TACGTA 1 cut(s) 1350
Eco24I GRGCYC 2 cut(s) 324, 1101
Eco31I GGTCTC 2 cut(s) 290, 353
Eco47I GGWCC 3 cut(s) 1030, 1169, 1236
Eco53kI GAGCTC 2 cut(s) 322, 1099
Eco88I CYCGRG 1 cut(s) 1100
EcoICRI GAGCTC 2 cut(s) 322, 1099
EcoRI GAATTC 2 cut(s) 170, 410
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 2 cut(s) 324, 1101
EgeI GGCGCC 1 cut(s) 433
EheI GGCGCC 1 cut(s) 433
FaeI CATG 6 cut(s) 263, 388, 595, 928, 1186, 1342
FaqI GGGAC 3 cut(s) 252, 739, 1249
FatI CATG 6 cut(s) 259, 384, 591, 924, 1182, 1338
FauI CCCGC 2 cut(s) 338, 1226
FbaI TGATCA 3 cut(s) 276, 525, 921
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 8 cut(s) 191, 342, 540, 710, 737, 788, 851, 1080
FokI GGATG 6 cut(s) 191, 614, 638, 1075, 1168, 1307
FriOI GRGCYC 2 cut(s) 324, 1101
Fsp4HI GCNGC 8 cut(s) 191, 342, 540, 710, 737, 788, 851, 1080
GlaI GCGC 4 cut(s) 433, 708, 786, 987
GluI GCNGC 8 cut(s) 191, 342, 540, 710, 737, 788, 851, 1080
GsaI CCCAGC 1 cut(s) 646
HaeII RGCGCY 2 cut(s) 435, 710
HaeIII GGCC 1 cut(s) 542
HapII CCGG 8 cut(s) 194, 291, 345, 674, 692, 1028, 1039, 1074
HgaI GACGC 1 cut(s) 892
HhaI GCGC 4 cut(s) 434, 709, 787, 988
Hin1I GRCGYC 4 cut(s) 315, 378, 432, 650
Hin1II CATG 6 cut(s) 263, 388, 595, 928, 1186, 1342
Hin6I GCGC 4 cut(s) 432, 707, 785, 986
HinP1I GCGC 4 cut(s) 432, 707, 785, 986
HinfI GANTC 5 cut(s) 336, 897, 1024, 1057, 1286
HpaII CCGG 8 cut(s) 194, 291, 345, 674, 692, 1028, 1039, 1074
Hpy166II GTNNAC 2 cut(s) 550, 796
Hpy188I TCNGA 6 cut(s) 908, 1002, 1062, 1086, 1142, 1325
Hpy188III TCNNGA 8 cut(s) 167, 208, 241, 350, 919, 1028, 1308, 1339
Hpy8I GTNNAC 2 cut(s) 550, 796
HpyAV CCTTC 7 cut(s) 112, 310, 427, 481, 553, 1087, 1301
HpyCH4III ACNGT 4 cut(s) 164, 266, 1111, 1240
HpyCH4IV ACGT 9 cut(s) 315, 378, 441, 546, 582, 650, 699, 834, 1349
HpyCH4V TGCA 5 cut(s) 634, 928, 1036, 1199, 1320
HpyF10VI GCNNNNNNNGC 6 cut(s) 291, 491, 500, 515, 900, 994
HpyF3I CTNAG 1 cut(s) 1254
HpySE526I ACGT 9 cut(s) 315, 378, 441, 546, 582, 650, 699, 834, 1349
Hsp92I GRCGYC 4 cut(s) 315, 378, 432, 650
Hsp92II CATG 6 cut(s) 263, 388, 595, 928, 1186, 1342
HspAI GCGC 4 cut(s) 432, 707, 785, 986
KasI GGCGCC 1 cut(s) 431
Kpn2I TCCGGA 1 cut(s) 1027
Ksp22I TGATCA 3 cut(s) 276, 525, 921
Kzo9I GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
LmnI GCTCC 4 cut(s) 393, 908, 1087, 1096
Lsp1109I GCAGC 5 cut(s) 177, 696, 723, 799, 837
LweI GCATC 3 cut(s) 459, 741, 1329
MaeII ACGT 9 cut(s) 315, 378, 441, 546, 582, 650, 699, 834, 1349
MaeIII GTNAC 4 cut(s) 3, 164, 772, 976
MalI GATC 6 cut(s) 278, 527, 839, 923, 941, 1265
MboI GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
MboII GAAGA 1 cut(s) 1273
MhlI GDGCHC 2 cut(s) 324, 1101
MluCI AATT 3 cut(s) 170, 254, 410
Mly113I GGCGCC 1 cut(s) 432
MlyI GAGTC 3 cut(s) 345, 891, 1033
MmeI TCCRAC 3 cut(s) 537, 886, 933
MroI TCCGGA 1 cut(s) 1027
MroXI GAANNNNTTC 2 cut(s) 470, 1290
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 646
MspI CCGG 8 cut(s) 194, 291, 345, 674, 692, 1028, 1039, 1074
MspR9I CCNGG 4 cut(s) 371, 674, 692, 1075
Mva1269I GAATGC 2 cut(s) 363, 422
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 6 cut(s) 291, 491, 500, 515, 900, 994
NarI GGCGCC 1 cut(s) 432
NciI CCSGG 3 cut(s) 674, 692, 1075
NdeII GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
NlaIII CATG 6 cut(s) 263, 388, 595, 928, 1186, 1342
NlaIV GGNNCC 3 cut(s) 433, 1083, 1237
NmeAIII GCCGAG 3 cut(s) 339, 408, 511
NmuCI GTSAC 4 cut(s) 3, 164, 772, 976
PaeR7I CTCGAG 1 cut(s) 1100
PagI TCATGA 1 cut(s) 1338
PcsI WCGNNNNNNNCGW 2 cut(s) 725, 737
PctI GAATGC 2 cut(s) 363, 422
PdmI GAANNNNTTC 2 cut(s) 470, 1290
PfeI GAWTC 2 cut(s) 1057, 1286
PflFI GACNNNGTC 3 cut(s) 380, 610, 1109
PinAI ACCGGT 1 cut(s) 1038
PkrI GCNGC 8 cut(s) 192, 343, 541, 711, 738, 789, 852, 1081
PleI GAGTC 3 cut(s) 344, 891, 1032
PluTI GGCGCC 1 cut(s) 435
PpsI GAGTC 3 cut(s) 344, 891, 1032
Ppu21I YACGTR 3 cut(s) 442, 547, 1350
Psp124BI GAGCTC 2 cut(s) 324, 1101
Psp1406I AACGTT 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 642
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 433, 1083, 1237
PspPI GGNCC 3 cut(s) 1030, 1169, 1236
PspXI VCTCGAGB 1 cut(s) 1100
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
PsyI GACNNNGTC 3 cut(s) 380, 610, 1109
PvuII CAGCTG 1 cut(s) 646
RsaI GTAC 3 cut(s) 716, 1043, 1348
RsaNI GTAC 3 cut(s) 715, 1042, 1347
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 2 cut(s) 324, 1101
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 8 cut(s) 191, 342, 540, 710, 737, 788, 851, 1080
Sau3AI GATC 6 cut(s) 276, 525, 837, 921, 939, 1263
Sau96I GGNCC 3 cut(s) 1030, 1169, 1236
SchI GAGTC 3 cut(s) 345, 891, 1033
ScrFI CCNGG 4 cut(s) 371, 674, 692, 1075
SduI GDGCHC 2 cut(s) 324, 1101
SfaNI GCATC 3 cut(s) 459, 741, 1329
SfoI GGCGCC 1 cut(s) 433
Sfr274I CTCGAG 1 cut(s) 1100
SgrAI CRCCGGYG 1 cut(s) 290
SinI GGWCC 3 cut(s) 1030, 1169, 1236
SlaI CTCGAG 1 cut(s) 1100
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 2 cut(s) 534, 1100
SmoI CTYRAG 2 cut(s) 534, 1100
SnaBI TACGTA 1 cut(s) 1350
Sse9I AATT 3 cut(s) 170, 254, 410
SsiI CCGC 9 cut(s) 70, 331, 342, 539, 677, 894, 901, 1079, 1233
SspDI GGCGCC 1 cut(s) 431
SstI GAGCTC 2 cut(s) 324, 1101
StyD4I CCNGG 4 cut(s) 369, 672, 690, 1073
TaaI ACNGT 4 cut(s) 164, 266, 1111, 1240
TaiI ACGT 9 cut(s) 318, 381, 444, 549, 585, 653, 702, 837, 1352
TaqI TCGA 6 cut(s) 240, 318, 351, 1055, 1101, 1269
TaqII GACCGA 1 cut(s) 1175
TasI AATT 3 cut(s) 170, 254, 410
TauI GCSGC 3 cut(s) 344, 542, 1082
TfiI GAWTC 2 cut(s) 1057, 1286
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 511, 1114
TseFI GTSAC 4 cut(s) 3, 164, 772, 976
TseI GCWGC 5 cut(s) 190, 709, 736, 787, 850
Tsp45I GTSAC 4 cut(s) 3, 164, 772, 976
TspDTI ATGAA 3 cut(s) 163, 1274, 1299
TspGWI ACGGA 2 cut(s) 117, 170
TspRI CASTG 2 cut(s) 511, 1114
Tth111I GACNNNGTC 3 cut(s) 380, 610, 1109
VpaK11BI GGWCC 3 cut(s) 1030, 1169, 1236
XapI RAATTY 2 cut(s) 170, 410
XhoI CTCGAG 1 cut(s) 1100
XmiI GTMKAC 1 cut(s) 795
XmnI GAANNNNTTC 2 cut(s) 470, 1290
ZraI GACGTC 3 cut(s) 316, 379, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.