MD17G1243300.v1.1

Clathrin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009774
Physical Location & Seq
Reverse (-)
29204351 .. 29206665
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1243300.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGTCGTCCTTCGACTCCTTCCCCAACGACGACGAATCCAACGACCTCCACAGCCACTCCCCCGCCGGCCAGCCCTTCGACGACGATGCCTACATGGCTCACGACCCCGGCCTTCCTTCCCATAACTACGACTCGTCGTTCAACCCTCCCACCCACCACGACGACTTCTCCCCCGCCGCCGTCGACCCTCCCCCTCCCCTCCACTCTGATTTCCAGGACGACGTCGTTCTCCCCGGTGGCGACGGCCACCACAACATTGATCAGCAGCAGCAGTCCCCCGAGGTGTACGGCTTCGGCATTTCCACCCCTAACCCGGAATTCGTGTCACCTTTTGATTCGGCGGTGCCCGAGGACAATGGCGTCGTGGGTGGCGGTGGCGGCATTGCTGATGACGACGGGGACATTTTCGCCTCCGGTGAGCCGGTACTGCCTCCGCCCGAGGAGATGCGGGAGGAAGGCTTTGCTCGCCGTGAGTGGCGTCGCCTAAATGTCATTCATCTAGAGGAAAAGGAAAAAAGGGAAAGGGAGATGCGGAACCGGATTATTGAGGAAGCTGAAGAATTTAAGCGGGCCTTCTACGAGAAACGGAAGCTCAATTGTGAAACTAACAAAGCTCACAACAGAGAAAGGGAGAAGCTGTACTTGGCCAACCAAGAGAAGTTCCACAAAGAAGCTGACAAACATTACTGGAAAGCAATAGCGGAGCTGATTCCTCGTGAGGTTCCTAATATTGAGAAGAAGAGAGGGAAGAAGGATCCTGACAAGAAGCCCTCAATTGTTGTCATCCAGGGCCCAAAACCTGGTAAACCGACTGATCTTTCAAGGATGCGGCAGATTTTCCTGAAGCTGAAACAGAACCCACCGCCGCACATGATGCCACCCCCACCTTCCAAAGATGGTAAGGATGCAAAGGGAGAAAAAGATGGAAAGGAGTCAAAGGATGGCAATGATACAAAAGATGCAAAGGGAGGAAAGGGCGCAAAGGAAGAAGGAACGGACGCAAAGGATGCCAAGGAAGGAGGAAAGGACACAAAGGATGCCAAGGAAGGAGGAAAGGATGCAAAGGAAGGAAAGGATGCGAAGAATGGAAAAATTAATTCACCAACCGCAGCTGGAGCTGCAGCTGCAAGCGCACCTGTTTCACCTGCCAAAGATGTCACTGCCAATGGTACTCCCGGCTCATCAAAACCAGAGGCTTCAGCTGAACCAGAGGGTGAGCAACATTTGGAAGCCACGCCAACTCCTGCGGAGTAA

Protein Analysis

418

Amino Acids

45.56

Weight (kDa)

5.24

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 148 - 241 4.3e-07 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1153
AasI GACNNNNNNGTC 1 cut(s) 359
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 1153
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 800
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 2 cut(s) 749, 762
AcoI YGGCCR 3 cut(s) 67, 244, 645
AcsI RAATTY 2 cut(s) 317, 560
AcuI CTGAAG 3 cut(s) 576, 863, 1182
AcyI GRCGYC 3 cut(s) 222, 360, 478
AfaI GTAC 4 cut(s) 287, 426, 641, 1171
AfiI CCNNNNNNNGG 2 cut(s) 313, 800
AgsI TTSAA 2 cut(s) 142, 822
AjnI CCWGG 3 cut(s) 213, 786, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AlwI GGATC 2 cut(s) 749, 762
Ama87I CYCGRG 3 cut(s) 278, 347, 437
AoxI GGCC 6 cut(s) 67, 109, 244, 570, 645, 790
ApaI GGGCCC 1 cut(s) 794
ApeKI GCWGC 6 cut(s) 265, 268, 1109, 1118, 1121, 1124
ApoI RAATTY 2 cut(s) 317, 560
AseI ATTAAT 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 980, 1133
AspS9I GGNCC 3 cut(s) 570, 790, 791
AsuC2I CCSGG 4 cut(s) 108, 234, 314, 1176
AsuHPI GGTGA 5 cut(s) 318, 428, 1092, 1134, 1226
AvaI CYCGRG 3 cut(s) 278, 347, 437
BaeGI GKGCMC 2 cut(s) 348, 794
BalI TGGCCA 1 cut(s) 647
BamHI GGATCC 1 cut(s) 754
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 794
BauI CACGAG 1 cut(s) 714
BbvI GCAGC 6 cut(s) 277, 280, 1105, 1111, 1121, 1133
BccI CCATC 3 cut(s) 890, 917, 935
BceAI ACGGC 4 cut(s) 164, 259, 304, 453
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 3 cut(s) 215, 788, 801
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 4 cut(s) 108, 234, 314, 1176
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 1 cut(s) 1119
BfuAI ACCTGC 1 cut(s) 1153
BglI GCCNNNNNGGC 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 108, 215, 234, 314, 788, 801, 1176
BmeT110I CYCGRG 3 cut(s) 278, 347, 437
BmgT120I GGNCC 3 cut(s) 570, 790, 791
BmiI GGNNCC 5 cut(s) 345, 536, 723, 756, 792
BmrFI CCNGG 7 cut(s) 108, 215, 234, 314, 788, 801, 1176
BpmI CTGGAG 1 cut(s) 1134
BpuMI CCSGG 4 cut(s) 108, 234, 314, 1176
BsaHI GRCGYC 3 cut(s) 222, 360, 478
BsaJI CCNNGG 8 cut(s) 106, 232, 279, 348, 438, 787, 1011, 1041
BsaWI WCCGGW 2 cut(s) 413, 537
BsaXI ACNNNNNCTCC 7 cut(s) 41, 71, 152, 182, 623, 653, 1225
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 800
Bse118I RCCGGY 2 cut(s) 65, 421
Bse1I ACTGG 1 cut(s) 692
Bse3DI GCAATG 2 cut(s) 381, 952
BseBI CCWGG 3 cut(s) 215, 788, 801
BseDI CCNNGG 8 cut(s) 106, 232, 279, 348, 438, 787, 1011, 1041
BseGI GGATG 8 cut(s) 783, 831, 910, 946, 1012, 1042, 1063, 1081
BseLI CCNNNNNNNGG 2 cut(s) 313, 800
BseMI GCAATG 2 cut(s) 381, 952
BseNI ACTGG 1 cut(s) 692
BseRI GAGGAG 1 cut(s) 455
BseSI GKGCMC 2 cut(s) 348, 794
BseXI GCAGC 6 cut(s) 277, 280, 1105, 1111, 1121, 1133
BshFI GGCC 6 cut(s) 69, 111, 246, 572, 647, 792
BshNI GGYRCC 1 cut(s) 343
BsiHKCI CYCGRG 3 cut(s) 278, 347, 437
BsiSI CCGG 8 cut(s) 66, 108, 234, 314, 414, 422, 538, 1176
BslFI GGGAC 2 cut(s) 259, 413
BslI CCNNNNNNNGG 2 cut(s) 313, 800
BsmFI GGGAC 2 cut(s) 259, 413
BsnI GGCC 6 cut(s) 69, 111, 246, 572, 647, 792
BsoBI CYCGRG 3 cut(s) 278, 347, 437
Bsp120I GGGCCC 1 cut(s) 790
Bsp1286I GDGCHC 2 cut(s) 348, 794
Bsp143I GATC 3 cut(s) 259, 754, 814
BspANI GGCC 6 cut(s) 69, 111, 246, 572, 647, 792
BspLI GGNNCC 5 cut(s) 345, 536, 723, 756, 792
BspMAI CTGCAG 1 cut(s) 1123
BspMI ACCTGC 1 cut(s) 1153
BspPI GGATC 2 cut(s) 749, 762
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 381, 952
BsrFI RCCGGY 2 cut(s) 65, 421
BsrI ACTGG 1 cut(s) 692
BssAI RCCGGY 2 cut(s) 65, 421
BssECI CCNNGG 8 cut(s) 106, 232, 279, 348, 438, 787, 1011, 1041
BssMI GATC 3 cut(s) 259, 754, 814
BssNI GRCGYC 3 cut(s) 222, 360, 478
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 2 cut(s) 1011, 1041
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 3 cut(s) 215, 788, 801
Bst6I CTCTTC 1 cut(s) 734
BstACI GRCGYC 3 cut(s) 222, 360, 478
BstAPI GCANNNNNTGC 2 cut(s) 874, 1007
BstC8I GCNNGC 5 cut(s) 67, 71, 466, 570, 1129
BstF5I GGATG 8 cut(s) 783, 831, 910, 946, 1012, 1042, 1063, 1081
BstHHI GCGC 2 cut(s) 980, 1133
BstKTI GATC 3 cut(s) 262, 757, 817
BstMBI GATC 3 cut(s) 259, 754, 814
BstNI CCWGG 3 cut(s) 215, 788, 801
BstSCI CCNGG 7 cut(s) 106, 213, 232, 312, 786, 799, 1174
BstSFI CTRYAG 1 cut(s) 1119
BstSLI GKGCMC 2 cut(s) 348, 794
BstV1I GCAGC 6 cut(s) 277, 280, 1105, 1111, 1121, 1133
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
BsuRI GGCC 6 cut(s) 69, 111, 246, 572, 647, 792
BtsCI GGATG 8 cut(s) 783, 831, 910, 946, 1012, 1042, 1063, 1081
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 1 cut(s) 1158
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 5 cut(s) 67, 71, 466, 570, 1129
CfoI GCGC 2 cut(s) 980, 1133
Cfr10I RCCGGY 2 cut(s) 65, 421
Cfr13I GGNCC 3 cut(s) 570, 790, 791
CseI GACGC 3 cut(s) 349, 467, 1007
CsiI ACCWGGT 1 cut(s) 799
Csp6I GTAC 4 cut(s) 286, 425, 640, 1170
CviAII CATG 2 cut(s) 94, 871
CviQI GTAC 4 cut(s) 286, 425, 640, 1170
DpnI GATC 3 cut(s) 261, 756, 816
DpnII GATC 3 cut(s) 259, 754, 814
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 3 cut(s) 67, 244, 645
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
EciI GGCGGA 1 cut(s) 423
Eco130I CCWWGG 2 cut(s) 1011, 1041
Eco24I GRGCYC 1 cut(s) 794
Eco57I CTGAAG 3 cut(s) 576, 863, 1182
Eco88I CYCGRG 3 cut(s) 278, 347, 437
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 213, 786, 799
EcoT14I CCWWGG 2 cut(s) 1011, 1041
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 1011, 1041
FaeI CATG 2 cut(s) 97, 874
FaiI YATR 3 cut(s) 95, 123, 872
FalI AAGNNNNNCTT 4 cut(s) 557, 589, 626, 658
FaqI GGGAC 2 cut(s) 259, 413
FatI CATG 2 cut(s) 93, 870
FauI CCCGC 4 cut(s) 70, 181, 441, 561
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 183
FokI GGATG 8 cut(s) 770, 838, 917, 953, 1019, 1049, 1070, 1088
FriOI GRGCYC 1 cut(s) 794
FspBI CTAG 1 cut(s) 500
GlaI GCGC 2 cut(s) 979, 1132
GsuI CTGGAG 1 cut(s) 1134
HaeIII GGCC 6 cut(s) 69, 111, 246, 572, 647, 792
HapII CCGG 8 cut(s) 66, 108, 234, 314, 414, 422, 538, 1176
HgaI GACGC 3 cut(s) 349, 467, 1007
HhaI GCGC 2 cut(s) 980, 1133
Hin1I GRCGYC 3 cut(s) 222, 360, 478
Hin1II CATG 2 cut(s) 97, 874
Hin6I GCGC 2 cut(s) 978, 1131
HinP1I GCGC 2 cut(s) 978, 1131
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 6 cut(s) 14, 35, 131, 335, 709, 932
HpaII CCGG 8 cut(s) 66, 108, 234, 314, 414, 422, 538, 1176
HphI GGTGA 5 cut(s) 318, 428, 1092, 1134, 1226
Hpy166II GTNNAC 3 cut(s) 184, 286, 806
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 5 cut(s) 101, 500, 716, 758, 841
Hpy8I GTNNAC 3 cut(s) 184, 286, 806
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 908, 962, 1061, 1121, 1127
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 3 cut(s) 222, 360, 478
Hsp92II CATG 2 cut(s) 97, 874
HspAI GCGC 2 cut(s) 978, 1131
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 754, 814
LmnI GCTCC 2 cut(s) 703, 1115
Lsp1109I GCAGC 6 cut(s) 277, 280, 1105, 1111, 1121, 1133
MabI ACCWGGT 1 cut(s) 799
MaeI CTAG 1 cut(s) 500
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 324, 1156
MalI GATC 3 cut(s) 261, 756, 816
MboI GATC 3 cut(s) 259, 754, 814
MboII GAAGA 6 cut(s) 569, 748, 751, 760, 998, 1093
MfeI CAATTG 2 cut(s) 595, 774
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 2 cut(s) 348, 794
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 6 cut(s) 317, 560, 595, 774, 1092, 1096
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 3 cut(s) 8, 125, 941
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 647
MroNI GCCGGC 1 cut(s) 65
MscI TGGCCA 1 cut(s) 647
MseI TTAA 2 cut(s) 564, 1095
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 3 cut(s) 1112, 1124, 1202
MspI CCGG 8 cut(s) 66, 108, 234, 314, 414, 422, 538, 1176
MspR9I CCNGG 7 cut(s) 108, 215, 234, 314, 788, 801, 1176
MunI CAATTG 2 cut(s) 595, 774
MvaI CCWGG 3 cut(s) 215, 788, 801
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 4 cut(s) 108, 234, 314, 1176
NdeII GATC 3 cut(s) 259, 754, 814
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 97, 874
NlaIV GGNNCC 5 cut(s) 345, 536, 723, 756, 792
NmuCI GTSAC 2 cut(s) 324, 1156
PaqCI CACCTGC 1 cut(s) 1153
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 3 cut(s) 35, 335, 709
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 800
PfoI TCCNGGA 1 cut(s) 213
PleI GAGTC 3 cut(s) 8, 125, 940
PpsI GAGTC 3 cut(s) 8, 125, 940
PshBI ATTAAT 1 cut(s) 1095
Psp6I CCWGG 3 cut(s) 213, 786, 799
PspGI CCWGG 3 cut(s) 213, 786, 799
PspN4I GGNNCC 5 cut(s) 345, 536, 723, 756, 792
PspOMI GGGCCC 1 cut(s) 790
PspPI GGNCC 3 cut(s) 570, 790, 791
PstI CTGCAG 1 cut(s) 1123
PsuI RGATCY 1 cut(s) 754
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 3 cut(s) 1112, 1124, 1202
RsaI GTAC 4 cut(s) 287, 426, 641, 1171
RsaNI GTAC 4 cut(s) 286, 425, 640, 1170
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 564, 1095
Sau3AI GATC 3 cut(s) 259, 754, 814
Sau96I GGNCC 3 cut(s) 570, 790, 791
SchI GAGTC 3 cut(s) 8, 125, 941
ScrFI CCNGG 7 cut(s) 108, 215, 234, 314, 788, 801, 1176
SduI GDGCHC 2 cut(s) 348, 794
SexAI ACCWGGT 1 cut(s) 799
SfcI CTRYAG 1 cut(s) 1119
Sse9I AATT 6 cut(s) 317, 560, 595, 774, 1092, 1096
SspI AATATT 1 cut(s) 730
SspMI CTAG 1 cut(s) 500
StyD4I CCNGG 7 cut(s) 106, 213, 232, 312, 786, 799, 1174
StyI CCWWGG 2 cut(s) 1011, 1041
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 12, 78, 183
TasI AATT 6 cut(s) 317, 560, 595, 774, 1092, 1096
TatI WGTACW 1 cut(s) 639
TauI GCSGC 4 cut(s) 179, 381, 832, 868
TfiI GAWTC 3 cut(s) 35, 335, 709
Tru1I TTAA 2 cut(s) 564, 1095
Tru9I TTAA 2 cut(s) 564, 1095
TscAI CASTG 1 cut(s) 1165
TseFI GTSAC 2 cut(s) 324, 1156
TseI GCWGC 6 cut(s) 265, 268, 1109, 1118, 1121, 1124
Tsp45I GTSAC 2 cut(s) 324, 1156
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 2 cut(s) 601, 1010
TspRI CASTG 1 cut(s) 1165
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 800
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 2 cut(s) 317, 560
XbaI TCTAGA 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 500
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.