Rroxscaffold_2G00117330

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009774
Physical Location & Seq
Forward (+)
45001368 .. 45004101
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117330.1

Sequence Viewer

Length: 1086 bp
ATGGGCTACGACTCGTCGTTCAACCCTACCACCCAAGTCGACTTCTCCTCCTCCGACCCACCACCTCCGCCTCAACCACTCCAATCAGATTTTCATGACGATGTTGTTCTTCCTAATGAGCAGCAGCACTTACCCGACCCCTACGGAGGAGGAGGGTTTGGAATTGAAGCCTCCAACCCTAATGACTACGCGTTGCCATTTGATTCGGCAGTTTCTGAGAGCAATGCAGATGGTAATAATGAGGGTGACATTTTCACTTCCGATGGGCCGGTGCTTCCGCCTCCAGAGCAAATGCGCGAGGAAGGCTTTGCACGCCGTGAGTGGCGCCGTCTAAATGCCATTCATCTTGAGGAAAAGGAGAAAAGAGAAAAAGAGATGCGAAACCAGATTATTGAAGAAGCTGAAGAGTTTAAACGGGCCTTTTATGAGAAAAGGAAGCTCAATTGTGAAACTAACAAAGCTAACAATAGAGAAAGAGAGAAGCTGTACTTGGCCAACCAAGAAAAGTTCCACAAAGAAGCTGACAAGCATTACTGGAAAGCAATAGCGGAGCTCATTCCTCGAGAGGTTCCTAATATTGAGAAGAAGAGAGGGAAAAAAGATTCTGATAAGAAGCCTTCAATCGTTGTCATCCAGGGTCCCAAACCTGGGAAACCTACAGATCTTTCACGAATGCGCCAAATTTTTCTGAAGCTAAAACAGACCCCTCCTCCACACATGATGCCTCCTCCACCAACACCTGCCAAAGATGGCAAGGATGCAAAGGACGAAAAGGATGTAAAGGAAGGGAAAGACACAAAGGACGAAAAGGATGGAATGGAAGGGAAAGACACAAAGGAAAGGAAGGATCCAAAGAATGAAAAAACAAATCCACCAACTACAGATGGAAAAACACTAGTCTCACCTTCCAAAGGTGTTACTGCTAATGGTACAAAGGATGCTTCAACACCAGAGGCTCCTACTGAACAAAAAGTTGAGCAAACTGGTGAACATGAGGGTCAGCTACCTGCTGAACCCAAAGGTGAGCAACCTTTAGAACCCAAGGGTGAGCAACTCGCTGATGCAGGGCCAACCCTTAAAGAGTGA

Protein Analysis

361

Amino Acids

40.49

Weight (kDa)

5.34

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 96 - 234 2.7e-06 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 748
Acc36I ACCTGC 2 cut(s) 748, 1015
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 39
AccII CGCG 2 cut(s) 191, 297
AciI CCGC 3 cut(s) 68, 278, 548
AclWI GGATC 2 cut(s) 842, 855
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 1 cut(s) 681
AcuI CTGAAG 2 cut(s) 423, 710
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 2 cut(s) 488, 931
AfiI CCNNNNNNNGG 4 cut(s) 146, 647, 648, 911
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 5 cut(s) 22, 167, 395, 621, 945
AhlI ACTAGT 1 cut(s) 895
AjnI CCWGG 2 cut(s) 633, 646
AjuI GAANNNNNNNTTGG 4 cut(s) 75, 107, 473, 505
AluBI AGCT 8 cut(s) 401, 439, 461, 484, 521, 553, 694, 1003
AluI AGCT 8 cut(s) 401, 439, 461, 484, 521, 553, 694, 1003
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 1 cut(s) 904
AlwI GGATC 2 cut(s) 842, 855
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 4 cut(s) 266, 417, 492, 1067
ApeKI GCWGC 2 cut(s) 121, 124
ApoI RAATTY 1 cut(s) 681
AspLEI GCGC 3 cut(s) 297, 327, 678
AspS9I GGNCC 4 cut(s) 266, 417, 638, 1067
AsuHPI GGTGA 5 cut(s) 257, 894, 998, 1034, 1058
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 1 cut(s) 638
BalI TGGCCA 1 cut(s) 494
BamHI GGATCC 1 cut(s) 847
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 133, 136
BccI CCATC 5 cut(s) 224, 257, 743, 806, 878
BceAI ACGGC 2 cut(s) 300, 312
BciT130I CCWGG 2 cut(s) 635, 648
BcoDI GTCTC 1 cut(s) 904
BcuI ACTAGT 1 cut(s) 895
BfaI CTAG 1 cut(s) 896
BfmI CTRYAG 2 cut(s) 657, 879
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 2 cut(s) 748, 1015
BglII AGATCT 1 cut(s) 661
BisI GCNGC 2 cut(s) 122, 125
BlsI GCNGC 2 cut(s) 123, 126
Bme1390I CCNGG 2 cut(s) 635, 648
Bme18I GGWCC 1 cut(s) 638
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 4 cut(s) 266, 417, 638, 1067
BmiI GGNNCC 6 cut(s) 326, 570, 639, 640, 849, 957
BmrFI CCNGG 2 cut(s) 635, 648
BmsI GCATC 5 cut(s) 366, 711, 748, 928, 1051
BpmI CTGGAG 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 634, 647, 1041
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 694, 724
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 647, 648, 911
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 539, 988
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 635, 648
BseDI CCNNGG 3 cut(s) 634, 647, 1041
BseGI GGATG 5 cut(s) 630, 763, 781, 817, 943
BseLI CCNNNNNNNGG 4 cut(s) 146, 647, 648, 911
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 2 cut(s) 539, 988
BseRI GAGGAG 6 cut(s) 37, 40, 162, 165, 699, 717
BseXI GCAGC 2 cut(s) 133, 136
Bsh1236I CGCG 2 cut(s) 191, 297
BshFI GGCC 4 cut(s) 268, 419, 494, 1069
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 4 cut(s) 146, 647, 648, 911
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 1 cut(s) 624
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 4 cut(s) 268, 419, 494, 1069
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 2 cut(s) 661, 847
BspACI CCGC 3 cut(s) 68, 278, 548
BspANI GGCC 4 cut(s) 268, 419, 494, 1069
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 2 cut(s) 191, 297
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 6 cut(s) 326, 570, 639, 640, 849, 957
BspMI ACCTGC 2 cut(s) 748, 1015
BspPI GGATC 2 cut(s) 842, 855
BspT107I GGYRCC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 2 cut(s) 539, 988
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 3 cut(s) 634, 647, 1041
BssMI GATC 2 cut(s) 661, 847
BssNI GRCGYC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 1041
Bst2UI CCWGG 2 cut(s) 635, 648
Bst6I CTCTTC 2 cut(s) 399, 581
BstACI GRCGYC 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 216
BstENI CCTNNNNNAGG 1 cut(s) 909
BstF5I GGATG 5 cut(s) 630, 763, 781, 817, 943
BstFNI CGCG 2 cut(s) 191, 297
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 3 cut(s) 297, 327, 678
BstKTI GATC 2 cut(s) 664, 850
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 2 cut(s) 661, 847
BstMWI GCNNNNNNNGC 3 cut(s) 286, 303, 312
BstNI CCWGG 2 cut(s) 635, 648
BstSCI CCNGG 2 cut(s) 633, 646
BstSFI CTRYAG 2 cut(s) 657, 879
BstUI CGCG 2 cut(s) 191, 297
BstV1I GCAGC 2 cut(s) 133, 136
BstX2I RGATCY 2 cut(s) 661, 847
BstYI RGATCY 2 cut(s) 661, 847
BsuRI GGCC 4 cut(s) 268, 419, 494, 1069
BtsCI GGATG 5 cut(s) 630, 763, 781, 817, 943
BveI ACCTGC 2 cut(s) 748, 1015
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 215
CciI TCATGA 1 cut(s) 94
CfoI GCGC 3 cut(s) 297, 327, 678
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 4 cut(s) 266, 417, 638, 1067
Csp6I GTAC 2 cut(s) 487, 930
CviAII CATG 3 cut(s) 95, 718, 992
CviQI GTAC 2 cut(s) 487, 930
DdeI CTNAG 1 cut(s) 216
DinI GGCGCC 1 cut(s) 326
DpnI GATC 2 cut(s) 663, 849
DpnII GATC 2 cut(s) 661, 847
DraI TTTAAA 1 cut(s) 412
DraIII CACNNNGTG 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 492
Eam1104I CTCTTC 2 cut(s) 399, 581
EarI CTCTTC 2 cut(s) 399, 581
EciI GGCGGA 2 cut(s) 57, 267
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 1041
Eco24I GRGCYC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 638
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 2 cut(s) 423, 710
Eco88I CYCGRG 1 cut(s) 561
EcoICRI GAGCTC 1 cut(s) 553
EcoNI CCTNNNNNAGG 1 cut(s) 909
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 633, 646
EcoT14I CCWWGG 1 cut(s) 1041
EcoT38I GRGCYC 1 cut(s) 555
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 1041
FaeI CATG 3 cut(s) 98, 721, 995
FaiI YATR 4 cut(s) 96, 426, 719, 993
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 1 cut(s) 624
FatI CATG 3 cut(s) 94, 717, 991
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 122, 125
FokI GGATG 5 cut(s) 617, 770, 788, 824, 950
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 2 cut(s) 122, 125
FspBI CTAG 1 cut(s) 896
GlaI GCGC 3 cut(s) 296, 326, 677
GluI GCNGC 2 cut(s) 122, 125
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 4 cut(s) 268, 419, 494, 1069
HapII CCGG 1 cut(s) 269
HhaI GCGC 3 cut(s) 297, 327, 678
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 3 cut(s) 98, 721, 995
Hin6I GCGC 3 cut(s) 295, 325, 676
HinP1I GCGC 3 cut(s) 295, 325, 676
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 3 cut(s) 11, 203, 602
HpaII CCGG 1 cut(s) 269
HphI GGTGA 5 cut(s) 257, 894, 998, 1034, 1058
Hpy166II GTNNAC 2 cut(s) 40, 989
Hpy188I TCNGA 6 cut(s) 55, 88, 217, 262, 607, 690
Hpy188III TCNNGA 5 cut(s) 95, 284, 347, 563, 669
Hpy8I GTNNAC 2 cut(s) 40, 989
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 6 cut(s) 296, 627, 779, 815, 838, 915
HpyCH4V TGCA 4 cut(s) 227, 311, 761, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 303, 312
HpyF3I CTNAG 1 cut(s) 216
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 3 cut(s) 98, 721, 995
HspAI GCGC 3 cut(s) 295, 325, 676
KasI GGCGCC 1 cut(s) 324
KflI GGGWCCC 1 cut(s) 638
Kzo9I GATC 2 cut(s) 661, 847
LmnI GCTCC 2 cut(s) 550, 961
Lsp1109I GCAGC 2 cut(s) 133, 136
LweI GCATC 5 cut(s) 366, 711, 748, 928, 1051
MaeI CTAG 1 cut(s) 896
MaeIII GTNAC 2 cut(s) 245, 916
MalI GATC 2 cut(s) 663, 849
MboI GATC 2 cut(s) 661, 847
MboII GAAGA 5 cut(s) 101, 407, 416, 595, 598
MfeI CAATTG 1 cut(s) 442
MflI RGATCY 2 cut(s) 661, 847
MhlI GDGCHC 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 3 cut(s) 162, 442, 681
MluI ACGCGT 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 494
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 78, 198
Mox20I TGGCCA 1 cut(s) 494
MscI TGGCCA 1 cut(s) 494
MseI TTAA 2 cut(s) 411, 1077
MslI CAYNNNNRTG 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 494
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 2 cut(s) 635, 648
MssI GTTTAAAC 1 cut(s) 412
MunI CAATTG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 2 cut(s) 635, 648
MvnI CGCG 2 cut(s) 191, 297
MwoI GCNNNNNNNGC 3 cut(s) 286, 303, 312
NarI GGCGCC 1 cut(s) 325
NdeII GATC 2 cut(s) 661, 847
NlaIII CATG 3 cut(s) 98, 721, 995
NlaIV GGNNCC 6 cut(s) 326, 570, 639, 640, 849, 957
NmuCI GTSAC 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 561
PagI TCATGA 1 cut(s) 94
PaqCI CACCTGC 1 cut(s) 748
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 2 cut(s) 203, 602
PkrI GCNGC 2 cut(s) 123, 126
PleI GAGTC 1 cut(s) 5
PluTI GGCGCC 1 cut(s) 328
PmeI GTTTAAAC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 5
PpuMI RGGWCCY 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 633, 646
PspGI CCWGG 2 cut(s) 633, 646
PspN4I GGNNCC 6 cut(s) 326, 570, 639, 640, 849, 957
PspPI GGNCC 4 cut(s) 266, 417, 638, 1067
PspPPI RGGWCCY 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 2 cut(s) 661, 847
RsaI GTAC 2 cut(s) 488, 931
RsaNI GTAC 2 cut(s) 487, 930
RseI CAYNNNNRTG 1 cut(s) 99
SacI GAGCTC 1 cut(s) 555
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 411, 1077
SatI GCNGC 2 cut(s) 122, 125
Sau3AI GATC 2 cut(s) 661, 847
Sau96I GGNCC 4 cut(s) 266, 417, 638, 1067
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 2 cut(s) 635, 648
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 5 cut(s) 366, 711, 748, 928, 1051
SfcI CTRYAG 2 cut(s) 657, 879
SfoI GGCGCC 1 cut(s) 326
Sfr274I CTCGAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 638
SlaI CTCGAG 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 2 cut(s) 347, 561
SmoI CTYRAG 2 cut(s) 347, 561
SpeI ACTAGT 1 cut(s) 895
Sse9I AATT 3 cut(s) 162, 442, 681
SsiI CCGC 3 cut(s) 68, 278, 548
SspDI GGCGCC 1 cut(s) 324
SspI AATATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 896
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 2 cut(s) 633, 646
StyI CCWWGG 1 cut(s) 1041
TaqI TCGA 2 cut(s) 39, 562
TasI AATT 3 cut(s) 162, 442, 681
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 2 cut(s) 203, 602
Tru1I TTAA 2 cut(s) 411, 1077
Tru9I TTAA 2 cut(s) 411, 1077
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 121, 124
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 3 cut(s) 83, 332, 873
TspGWI ACGGA 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 638
XagI CCTNNNNNAGG 1 cut(s) 909
XapI RAATTY 1 cut(s) 681
XhoI CTCGAG 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.