Rh2DG347200

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009774
Physical Location & Seq
Reverse (-)
47997225 .. 48000651
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG347200.1

Sequence Viewer

Length: 1137 bp
ATGTCGTCCTTCGACTCATTTCCTAACGAGGACGAACCCCATACCCTATCCCCCACCATTCAACCTTTCAACGACGATGGTTACATGGGCTACGACCCGTCGTTCAACCCTCCCACCCAAGTCGACTTCTCCTCCTCCGACCCACCACCTCCGCCTCAACCACTCCACTCGGATTTTCATGACGATGTTGTTCTTCCCAACGAGCAGCAGCAGCAGCAGCACTCACCCGACCCCTATGGAGGAGGAGGGTTTGGAATTGAAGCCTCCAACCCCAACCCCAATGACTACGCGTTGCCATTTGATTCGGCGGTTTCTGAGAGCAATGCAGATGGTAATAATGAGGGTGACATTTTCACCTCCGATGGGCCGGTGCTTCCGCCTCCAGAGCAAATGCGTGAGGAAGGCTTTGCACGCCGTGAGTGGCGCCGTCTAAATGCCATTCATCTTGAGGAAAAGGAGAAAAGAGAAAAAGATATGCGAAACCAGATTATTGAAGAAGCTGAAGAGTTTAAACGGGCCTTTTATGAGAAAAGGAAGCTCAATTGTGAAACTAACAAAGCTAACAATAGAGAAAGAGAGAAGCTGTACTTGGCCAACCAAGAAAAGTTCCACAAAGAAGCTGACAAGCATTACTGGAAAGCAATAGCGGAGCTCATTCCTCGAGAGGTTCCTAATATTGAGAAGAAGAGAGGTAAAAAAGATCCTGATAAGAAGCCTTCAATCGTTGTCATCCAGGGTCCCAAACCTGGGAAACCTACAGATCTTTCACGAATGCGCCAAATTTTTCTGAAGCTAAAACAGACCCCTCCACCACACATGATGCCTCCTCCACCGACACCTGCCAAAGATGGCAATGATGCAAAGGACGGAAAGGATGGAAAGGAAGGGAAAGACACAAAGGATGGAAAGGAAGGGAAAGACACAAAGGAAAGGAAGGATCCAAAGAATGAAAAAATAAATACACCAACTGCAGTTGGAATAACACCAGTCTCACCTTCCAAAGGTGTTACTGCTAATGGTACAAAGGATGCTTCAACACCAGAGGCTCCTGCTGAACCAAAAGTTGAGCAAACTGGTGAACATGAAGGTCAACTACCTGCTGAACCCAAAGGAAGAATAAGCACAGTCATATATTGA

Protein Analysis

378

Amino Acids

42.31

Weight (kDa)

5.41

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 118 - 267 2.3e-06 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 847
Acc36I ACCTGC 2 cut(s) 847, 1105
AccB1I GGYRCC 1 cut(s) 423
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 290
AciI CCGC 4 cut(s) 152, 308, 377, 647
AclWI GGATC 3 cut(s) 695, 932, 945
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 2 cut(s) 522, 809
AcyI GRCGYC 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 416
AfaI GTAC 2 cut(s) 587, 1021
AfiI CCNNNNNNNGG 5 cut(s) 239, 363, 746, 747, 1001
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 7 cut(s) 62, 70, 106, 260, 494, 720, 1035
AjnI CCWGG 2 cut(s) 732, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 7 cut(s) 500, 538, 560, 583, 620, 652, 793
AluI AGCT 7 cut(s) 500, 538, 560, 583, 620, 652, 793
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 1 cut(s) 994
AlwI GGATC 3 cut(s) 695, 932, 945
Ama87I CYCGRG 1 cut(s) 660
AoxI GGCC 3 cut(s) 365, 516, 591
ApeKI GCWGC 5 cut(s) 205, 208, 211, 214, 217
ApoI RAATTY 1 cut(s) 780
AspLEI GCGC 2 cut(s) 426, 777
AspS9I GGNCC 3 cut(s) 365, 516, 737
AsuHPI GGTGA 5 cut(s) 216, 346, 356, 984, 1088
AvaI CYCGRG 1 cut(s) 660
AvaII GGWCC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 593
BamHI GGATCC 1 cut(s) 937
BanI GGYRCC 1 cut(s) 423
BanII GRGCYC 1 cut(s) 654
BarI GAAGNNNNNNTAC 2 cut(s) 1077, 1109
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 5 cut(s) 217, 220, 223, 226, 229
BccI CCATC 6 cut(s) 71, 323, 356, 842, 869, 896
BceAI ACGGC 2 cut(s) 399, 411
BciT130I CCWGG 2 cut(s) 734, 747
BcoDI GTCTC 1 cut(s) 994
BfmI CTRYAG 2 cut(s) 756, 969
BfoI RGCGCY 1 cut(s) 427
BfuAI ACCTGC 2 cut(s) 847, 1105
BglII AGATCT 1 cut(s) 760
BisI GCNGC 5 cut(s) 206, 209, 212, 215, 218
BlsI GCNGC 5 cut(s) 207, 210, 213, 216, 219
Bme1390I CCNGG 2 cut(s) 734, 747
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 1 cut(s) 660
BmgT120I GGNCC 3 cut(s) 365, 516, 737
BmiI GGNNCC 6 cut(s) 425, 669, 738, 739, 939, 1047
BmrFI CCNGG 2 cut(s) 734, 747
BmsI GCATC 3 cut(s) 810, 847, 1018
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 467
BsaHI GRCGYC 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 733, 746
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 5 cut(s) 239, 363, 746, 747, 1001
Bse118I RCCGGY 1 cut(s) 367
Bse1I ACTGG 3 cut(s) 638, 986, 1078
Bse3DI GCAATG 2 cut(s) 328, 859
BseBI CCWGG 2 cut(s) 734, 747
BseDI CCNNGG 2 cut(s) 733, 746
BseGI GGATG 4 cut(s) 729, 880, 907, 1033
BseLI CCNNNNNNNGG 5 cut(s) 239, 363, 746, 747, 1001
BseMI GCAATG 2 cut(s) 328, 859
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 3 cut(s) 638, 986, 1078
BseRI GAGGAG 5 cut(s) 121, 124, 255, 258, 816
BseXI GCAGC 5 cut(s) 217, 220, 223, 226, 229
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 3 cut(s) 367, 518, 593
BshNI GGYRCC 1 cut(s) 423
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 1 cut(s) 368
BslFI GGGAC 1 cut(s) 723
BslI CCNNNNNNNGG 5 cut(s) 239, 363, 746, 747, 1001
BsmAI GTCTC 1 cut(s) 994
BsmFI GGGAC 1 cut(s) 723
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 367, 518, 593
BsoBI CYCGRG 1 cut(s) 660
Bsp1286I GDGCHC 1 cut(s) 654
Bsp143I GATC 3 cut(s) 700, 760, 937
BspACI CCGC 4 cut(s) 152, 308, 377, 647
BspANI GGCC 3 cut(s) 367, 518, 593
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 1 cut(s) 290
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 6 cut(s) 425, 669, 738, 739, 939, 1047
BspMAI CTGCAG 1 cut(s) 973
BspMI ACCTGC 2 cut(s) 847, 1105
BspPI GGATC 3 cut(s) 695, 932, 945
BspT107I GGYRCC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 328, 859
BsrFI RCCGGY 1 cut(s) 367
BsrI ACTGG 3 cut(s) 638, 986, 1078
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 733, 746
BssMI GATC 3 cut(s) 700, 760, 937
BssNI GRCGYC 1 cut(s) 424
Bst2UI CCWGG 2 cut(s) 734, 747
Bst4CI ACNGT 1 cut(s) 1126
Bst6I CTCTTC 2 cut(s) 498, 680
BstACI GRCGYC 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 1 cut(s) 315
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 729, 880, 907, 1033
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 426, 777
BstKTI GATC 3 cut(s) 703, 763, 940
BstMAI GTCTC 1 cut(s) 994
BstMBI GATC 3 cut(s) 700, 760, 937
BstMWI GCNNNNNNNGC 5 cut(s) 211, 214, 217, 385, 411
BstNI CCWGG 2 cut(s) 734, 747
BstSCI CCNGG 2 cut(s) 732, 745
BstSFI CTRYAG 2 cut(s) 756, 969
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 5 cut(s) 217, 220, 223, 226, 229
BstX2I RGATCY 3 cut(s) 700, 760, 937
BstYI RGATCY 3 cut(s) 700, 760, 937
BsuRI GGCC 3 cut(s) 367, 518, 593
BtsCI GGATG 4 cut(s) 729, 880, 907, 1033
BveI ACCTGC 2 cut(s) 847, 1105
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 1 cut(s) 178
CfoI GCGC 2 cut(s) 426, 777
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 365, 516, 737
Csp6I GTAC 2 cut(s) 586, 1020
CviAII CATG 4 cut(s) 85, 179, 817, 1082
CviQI GTAC 2 cut(s) 586, 1020
DdeI CTNAG 1 cut(s) 315
DinI GGCGCC 1 cut(s) 425
DpnI GATC 3 cut(s) 702, 762, 939
DpnII GATC 3 cut(s) 700, 760, 937
DraI TTTAAA 1 cut(s) 511
DraIII CACNNNGTG 1 cut(s) 416
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 2 cut(s) 498, 680
EarI CTCTTC 2 cut(s) 498, 680
EciI GGCGGA 2 cut(s) 141, 366
Ecl136II GAGCTC 1 cut(s) 652
Eco24I GRGCYC 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 737
Eco53kI GAGCTC 1 cut(s) 652
Eco57I CTGAAG 2 cut(s) 522, 809
Eco88I CYCGRG 1 cut(s) 660
EcoICRI GAGCTC 1 cut(s) 652
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 737
EcoRII CCWGG 2 cut(s) 732, 745
EcoT38I GRGCYC 1 cut(s) 654
EgeI GGCGCC 1 cut(s) 425
EheI GGCGCC 1 cut(s) 425
FaeI CATG 4 cut(s) 88, 182, 820, 1085
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 1 cut(s) 723
FatI CATG 4 cut(s) 84, 178, 816, 1081
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 206, 209, 212, 215, 218
FokI GGATG 4 cut(s) 716, 887, 914, 1040
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 5 cut(s) 206, 209, 212, 215, 218
GlaI GCGC 2 cut(s) 425, 776
GluI GCNGC 5 cut(s) 206, 209, 212, 215, 218
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 3 cut(s) 367, 518, 593
HapII CCGG 1 cut(s) 368
HhaI GCGC 2 cut(s) 426, 777
Hin1I GRCGYC 1 cut(s) 424
Hin1II CATG 4 cut(s) 88, 182, 820, 1085
Hin6I GCGC 2 cut(s) 424, 775
HinP1I GCGC 2 cut(s) 424, 775
HincII GTYRAC 2 cut(s) 124, 1091
HindII GTYRAC 2 cut(s) 124, 1091
HinfI GANTC 2 cut(s) 14, 302
HpaII CCGG 1 cut(s) 368
HphI GGTGA 5 cut(s) 216, 346, 356, 984, 1088
Hpy166II GTNNAC 3 cut(s) 124, 1079, 1091
Hpy188I TCNGA 5 cut(s) 139, 172, 316, 361, 789
Hpy188III TCNNGA 6 cut(s) 179, 383, 446, 662, 704, 768
Hpy8I GTNNAC 3 cut(s) 124, 1079, 1091
Hpy99I CGWCG 2 cut(s) 77, 103
HpyAV CCTTC 8 cut(s) 19, 395, 726, 878, 905, 928, 1005, 1079
HpyCH4III ACNGT 1 cut(s) 1126
HpyCH4V TGCA 4 cut(s) 326, 410, 860, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 211, 214, 217, 385, 411
HpyF3I CTNAG 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 424
Hsp92II CATG 4 cut(s) 88, 182, 820, 1085
HspAI GCGC 2 cut(s) 424, 775
KasI GGCGCC 1 cut(s) 423
KflI GGGWCCC 1 cut(s) 737
Kzo9I GATC 3 cut(s) 700, 760, 937
LmnI GCTCC 2 cut(s) 649, 1051
Lsp1109I GCAGC 5 cut(s) 217, 220, 223, 226, 229
LweI GCATC 3 cut(s) 810, 847, 1018
MaeIII GTNAC 3 cut(s) 80, 344, 1006
MalI GATC 3 cut(s) 702, 762, 939
MboI GATC 3 cut(s) 700, 760, 937
MboII GAAGA 6 cut(s) 185, 506, 515, 694, 697, 1125
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 3 cut(s) 700, 760, 937
MhlI GDGCHC 1 cut(s) 654
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 3 cut(s) 255, 541, 780
MluI ACGCGT 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 593
Mly113I GGCGCC 1 cut(s) 424
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 3 cut(s) 162, 291, 955
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MseI TTAA 1 cut(s) 510
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 593
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 2 cut(s) 734, 747
MssI GTTTAAAC 1 cut(s) 511
MunI CAATTG 1 cut(s) 541
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 2 cut(s) 734, 747
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 5 cut(s) 211, 214, 217, 385, 411
NarI GGCGCC 1 cut(s) 424
NdeII GATC 3 cut(s) 700, 760, 937
NlaIII CATG 4 cut(s) 88, 182, 820, 1085
NlaIV GGNNCC 6 cut(s) 425, 669, 738, 739, 939, 1047
NmuCI GTSAC 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 660
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 847
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 1 cut(s) 302
PkrI GCNGC 5 cut(s) 207, 210, 213, 216, 219
PleI GAGTC 1 cut(s) 8
PluTI GGCGCC 1 cut(s) 427
PmeI GTTTAAAC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 654
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 2 cut(s) 732, 745
PspGI CCWGG 2 cut(s) 732, 745
PspN4I GGNNCC 6 cut(s) 425, 669, 738, 739, 939, 1047
PspPI GGNCC 3 cut(s) 365, 516, 737
PspPPI RGGWCCY 1 cut(s) 737
PstI CTGCAG 1 cut(s) 973
PsuI RGATCY 3 cut(s) 700, 760, 937
RsaI GTAC 2 cut(s) 587, 1021
RsaNI GTAC 2 cut(s) 586, 1020
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 654
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 5 cut(s) 206, 209, 212, 215, 218
Sau3AI GATC 3 cut(s) 700, 760, 937
Sau96I GGNCC 3 cut(s) 365, 516, 737
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 734, 747
SduI GDGCHC 1 cut(s) 654
SfaNI GCATC 3 cut(s) 810, 847, 1018
SfcI CTRYAG 2 cut(s) 756, 969
SfoI GGCGCC 1 cut(s) 425
Sfr274I CTCGAG 1 cut(s) 660
SinI GGWCC 1 cut(s) 737
SlaI CTCGAG 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 446, 660
SmoI CTYRAG 2 cut(s) 446, 660
Sse9I AATT 3 cut(s) 255, 541, 780
SsiI CCGC 4 cut(s) 152, 308, 377, 647
SspDI GGCGCC 1 cut(s) 423
SspI AATATT 1 cut(s) 676
SstI GAGCTC 1 cut(s) 654
StyD4I CCNGG 2 cut(s) 732, 745
TaaI ACNGT 1 cut(s) 1126
TaqI TCGA 3 cut(s) 12, 123, 661
TasI AATT 3 cut(s) 255, 541, 780
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 5 cut(s) 205, 208, 211, 214, 217
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 167, 431, 963, 1098
TspGWI ACGGA 1 cut(s) 882
VpaK11BI GGWCC 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 1 cut(s) 780
XhoI CTCGAG 1 cut(s) 660
XmiI GTMKAC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.