Rroxscaffold_2G00117300

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009774
Physical Location & Seq
Forward (+)
44913697 .. 44916436
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117300.1

Sequence Viewer

Length: 1170 bp
ATGTCATCCTTCAACTCATTTCCTAACGAGGACGAACCCCAGAACCTGTCCCCCACCATTCAACCTTTCAACGACGATGGTTACATGGGCTACGACTCGTCGTTCAACCCTACCACCCAAGTCGACTTCTCCTCCTCCGACCCACCACCTCCGCCTCAACCACTCCAATCAGATTTTCATGACGATGTTGTTCTTCCTAATGAGCAGCAGCACTTACCCGACCCCTACGGAGGAGGAGGGTTTGGAATTGAAGCCTCCAACCCTAATGACTACGCGTTGCCATTTGATTCGGCAGTTTCTGAGAGCAATGCAGATGGTAATAATGAGGGTGACATTTTCACTTCCGATGGGCCGGTGCTTCCGCCTCCAGAGCAAATGCGCGAGGAAGGCTTTGCACGCCGTGAGTGGCGCCGTCTAAATGCCATTCATCTTGAGGAAAAGGAGAAAAGAGAAAAAGAGATGCGAAACCAGATTATTGAAGAAGCTGAAGAGTTTAAACGGGCCTTTTATGAGAAAAGGAAGCTCAATTGTGAAACTAACAAAGCTAACAATAGAGAAAGAGAGAAGCTGTACTTGGCCAACCAAGAAAAGTTCCACAAAGAAGCTGACAAGCATTACTGGAAAGCAATAGCGGAGCTCATTCCTCGAGAGGTTCCTAATATTGAGAAGAAGAGAGGGAAAAAAGATTCTGATAAGAAGCCTTCAATCGTTGTCATCCAGGGTCCCAAACCTGGGAAACCTACAGATCTTTCACGAATGCGCCAAATTTTTCTGAAGCTAAAACAGACCCCTCCTCCACACATGATGCCTCCTCCACCAACACCTGCCAAAGATGGCAAGGATGCAAAGGACGAAAAGGATGTAAAGGAAGGGAAAGACACAAAGGACGAAAAGGATGGAATGGAAGGGAAAGACACAAAGGAAAGGAAGGATCCAAAGAATGAAAAAACAAATCCACCAACTACAGATGGAAAAACACTAGTCTCACCTTCCAAAGGTGTTACTGCTAATGGTACAAAGGATGCTTCAACACCAGAGGCTCCTACTGAACAAAAAGTTGAGCAAACTGGTGAACATGAGGGTCAGCTACCTGCTGAACCCAAAGGTGAGCAACCTTTAGAACCCAAGGGTGAGCAACTCGCTGATGCAGGGCCAACCCTTAAAGAGTGA

Protein Analysis

389

Amino Acids

43.66

Weight (kDa)

5.1

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 124 - 262 2.9e-06 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 832
Acc36I ACCTGC 2 cut(s) 832, 1099
AccB1I GGYRCC 1 cut(s) 408
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 275, 381
AciI CCGC 3 cut(s) 152, 362, 632
AclWI GGATC 2 cut(s) 926, 939
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 765
AcuI CTGAAG 2 cut(s) 507, 794
AcyI GRCGYC 1 cut(s) 409
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 2 cut(s) 572, 1015
AfiI CCNNNNNNNGG 4 cut(s) 230, 731, 732, 995
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 8 cut(s) 13, 62, 70, 106, 251, 479, 705, 1029
AhlI ACTAGT 1 cut(s) 979
AjnI CCWGG 2 cut(s) 717, 730
AjuI GAANNNNNNNTTGG 4 cut(s) 159, 191, 557, 589
AluBI AGCT 8 cut(s) 485, 523, 545, 568, 605, 637, 778, 1087
AluI AGCT 8 cut(s) 485, 523, 545, 568, 605, 637, 778, 1087
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 988
AlwI GGATC 2 cut(s) 926, 939
AlwNI CAGNNNCTG 2 cut(s) 46, 299
Ama87I CYCGRG 1 cut(s) 645
AoxI GGCC 4 cut(s) 350, 501, 576, 1151
ApeKI GCWGC 2 cut(s) 205, 208
ApoI RAATTY 1 cut(s) 765
AspLEI GCGC 3 cut(s) 381, 411, 762
AspS9I GGNCC 4 cut(s) 350, 501, 722, 1151
AsuHPI GGTGA 5 cut(s) 341, 978, 1082, 1118, 1142
AvaI CYCGRG 1 cut(s) 645
AvaII GGWCC 1 cut(s) 722
BalI TGGCCA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 931
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 217, 220
BccI CCATC 6 cut(s) 71, 308, 341, 827, 890, 962
BceAI ACGGC 2 cut(s) 384, 396
BciT130I CCWGG 2 cut(s) 719, 732
BcoDI GTCTC 1 cut(s) 988
BcuI ACTAGT 1 cut(s) 979
BfaI CTAG 1 cut(s) 980
BfmI CTRYAG 2 cut(s) 741, 963
BfoI RGCGCY 1 cut(s) 412
BfuAI ACCTGC 2 cut(s) 832, 1099
BglII AGATCT 1 cut(s) 745
BisI GCNGC 2 cut(s) 206, 209
BlsI GCNGC 2 cut(s) 207, 210
Bme1390I CCNGG 2 cut(s) 719, 732
Bme18I GGWCC 1 cut(s) 722
BmeT110I CYCGRG 1 cut(s) 645
BmgT120I GGNCC 4 cut(s) 350, 501, 722, 1151
BmiI GGNNCC 6 cut(s) 410, 654, 723, 724, 933, 1041
BmrFI CCNGG 2 cut(s) 719, 732
BmsI GCATC 5 cut(s) 450, 795, 832, 1012, 1135
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 3 cut(s) 718, 731, 1125
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 778, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 731, 732, 995
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 623, 1072
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 719, 732
BseDI CCNNGG 3 cut(s) 718, 731, 1125
BseGI GGATG 6 cut(s) 5, 714, 847, 865, 901, 1027
BseLI CCNNNNNNNGG 4 cut(s) 230, 731, 732, 995
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 623, 1072
BseRI GAGGAG 6 cut(s) 121, 124, 246, 249, 783, 801
BseXI GCAGC 2 cut(s) 217, 220
Bsh1236I CGCG 2 cut(s) 275, 381
BshFI GGCC 4 cut(s) 352, 503, 578, 1153
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 1 cut(s) 645
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 2 cut(s) 34, 708
BslI CCNNNNNNNGG 4 cut(s) 230, 731, 732, 995
BsmAI GTCTC 1 cut(s) 988
BsmFI GGGAC 2 cut(s) 34, 708
BsmI GAATGC 1 cut(s) 762
BsnI GGCC 4 cut(s) 352, 503, 578, 1153
BsoBI CYCGRG 1 cut(s) 645
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 2 cut(s) 745, 931
BspACI CCGC 3 cut(s) 152, 362, 632
BspANI GGCC 4 cut(s) 352, 503, 578, 1153
BspCNI CTCAG 1 cut(s) 292
BspFNI CGCG 2 cut(s) 275, 381
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 6 cut(s) 410, 654, 723, 724, 933, 1041
BspMI ACCTGC 2 cut(s) 832, 1099
BspPI GGATC 2 cut(s) 926, 939
BspT107I GGYRCC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 2 cut(s) 623, 1072
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 3 cut(s) 718, 731, 1125
BssMI GATC 2 cut(s) 745, 931
BssNI GRCGYC 1 cut(s) 409
BssT1I CCWWGG 1 cut(s) 1125
Bst2UI CCWGG 2 cut(s) 719, 732
Bst6I CTCTTC 2 cut(s) 483, 665
BstACI GRCGYC 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 1 cut(s) 300
BstENI CCTNNNNNAGG 1 cut(s) 993
BstF5I GGATG 6 cut(s) 5, 714, 847, 865, 901, 1027
BstFNI CGCG 2 cut(s) 275, 381
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 3 cut(s) 381, 411, 762
BstKTI GATC 2 cut(s) 748, 934
BstMAI GTCTC 1 cut(s) 988
BstMBI GATC 2 cut(s) 745, 931
BstMWI GCNNNNNNNGC 3 cut(s) 370, 387, 396
BstNI CCWGG 2 cut(s) 719, 732
BstSCI CCNGG 2 cut(s) 717, 730
BstSFI CTRYAG 2 cut(s) 741, 963
BstUI CGCG 2 cut(s) 275, 381
BstV1I GCAGC 2 cut(s) 217, 220
BstX2I RGATCY 2 cut(s) 745, 931
BstYI RGATCY 2 cut(s) 745, 931
BsuRI GGCC 4 cut(s) 352, 503, 578, 1153
BtsCI GGATG 6 cut(s) 5, 714, 847, 865, 901, 1027
BveI ACCTGC 2 cut(s) 832, 1099
Cac8I GCNNGC 1 cut(s) 397
CaiI CAGNNNCTG 2 cut(s) 46, 299
CciI TCATGA 1 cut(s) 178
CfoI GCGC 3 cut(s) 381, 411, 762
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 4 cut(s) 350, 501, 722, 1151
Csp6I GTAC 2 cut(s) 571, 1014
CviAII CATG 4 cut(s) 85, 179, 802, 1076
CviQI GTAC 2 cut(s) 571, 1014
DdeI CTNAG 1 cut(s) 300
DinI GGCGCC 1 cut(s) 410
DpnI GATC 2 cut(s) 747, 933
DpnII GATC 2 cut(s) 745, 931
DraI TTTAAA 1 cut(s) 496
DraIII CACNNNGTG 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 483, 665
EarI CTCTTC 2 cut(s) 483, 665
EciI GGCGGA 2 cut(s) 141, 351
Ecl136II GAGCTC 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 1125
Eco24I GRGCYC 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 637
Eco57I CTGAAG 2 cut(s) 507, 794
Eco88I CYCGRG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 993
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 717, 730
EcoT14I CCWWGG 1 cut(s) 1125
EcoT38I GRGCYC 1 cut(s) 639
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 1125
FaeI CATG 4 cut(s) 88, 182, 805, 1079
FaiI YATR 5 cut(s) 86, 180, 510, 803, 1077
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 2 cut(s) 34, 708
FatI CATG 4 cut(s) 84, 178, 801, 1075
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 206, 209
FokI GGATG 5 cut(s) 701, 854, 872, 908, 1034
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 2 cut(s) 206, 209
FspBI CTAG 1 cut(s) 980
GlaI GCGC 3 cut(s) 380, 410, 761
GluI GCNGC 2 cut(s) 206, 209
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 4 cut(s) 352, 503, 578, 1153
HapII CCGG 1 cut(s) 353
HhaI GCGC 3 cut(s) 381, 411, 762
Hin1I GRCGYC 1 cut(s) 409
Hin1II CATG 4 cut(s) 88, 182, 805, 1079
Hin6I GCGC 3 cut(s) 379, 409, 760
HinP1I GCGC 3 cut(s) 379, 409, 760
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 3 cut(s) 95, 287, 686
HpaII CCGG 1 cut(s) 353
HphI GGTGA 5 cut(s) 341, 978, 1082, 1118, 1142
Hpy166II GTNNAC 2 cut(s) 124, 1073
Hpy188I TCNGA 6 cut(s) 139, 172, 301, 346, 691, 774
Hpy188III TCNNGA 5 cut(s) 179, 368, 431, 647, 753
Hpy8I GTNNAC 2 cut(s) 124, 1073
Hpy99I CGWCG 2 cut(s) 77, 103
HpyAV CCTTC 7 cut(s) 19, 380, 711, 863, 899, 922, 999
HpyCH4V TGCA 4 cut(s) 311, 395, 845, 1148
HpyF10VI GCNNNNNNNGC 3 cut(s) 370, 387, 396
HpyF3I CTNAG 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 409
Hsp92II CATG 4 cut(s) 88, 182, 805, 1079
HspAI GCGC 3 cut(s) 379, 409, 760
KasI GGCGCC 1 cut(s) 408
KflI GGGWCCC 1 cut(s) 722
Kzo9I GATC 2 cut(s) 745, 931
LmnI GCTCC 2 cut(s) 634, 1045
Lsp1109I GCAGC 2 cut(s) 217, 220
LweI GCATC 5 cut(s) 450, 795, 832, 1012, 1135
MaeI CTAG 1 cut(s) 980
MaeIII GTNAC 3 cut(s) 80, 329, 1000
MalI GATC 2 cut(s) 747, 933
MboI GATC 2 cut(s) 745, 931
MboII GAAGA 5 cut(s) 185, 491, 500, 679, 682
MfeI CAATTG 1 cut(s) 526
MflI RGATCY 2 cut(s) 745, 931
MhlI GDGCHC 1 cut(s) 639
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 3 cut(s) 246, 526, 765
MluI ACGCGT 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 578
Mly113I GGCGCC 1 cut(s) 409
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 162, 282
Mox20I TGGCCA 1 cut(s) 578
MscI TGGCCA 1 cut(s) 578
MseI TTAA 2 cut(s) 495, 1161
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 578
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 719, 732
MssI GTTTAAAC 1 cut(s) 496
MunI CAATTG 1 cut(s) 526
Mva1269I GAATGC 1 cut(s) 762
MvaI CCWGG 2 cut(s) 719, 732
MvnI CGCG 2 cut(s) 275, 381
MwoI GCNNNNNNNGC 3 cut(s) 370, 387, 396
NarI GGCGCC 1 cut(s) 409
NdeII GATC 2 cut(s) 745, 931
NlaIII CATG 4 cut(s) 88, 182, 805, 1079
NlaIV GGNNCC 6 cut(s) 410, 654, 723, 724, 933, 1041
NmuCI GTSAC 1 cut(s) 329
PaeR7I CTCGAG 1 cut(s) 645
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 832
PctI GAATGC 1 cut(s) 762
PfeI GAWTC 2 cut(s) 287, 686
PkrI GCNGC 2 cut(s) 207, 210
PleI GAGTC 1 cut(s) 89
PluTI GGCGCC 1 cut(s) 412
PmeI GTTTAAAC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 89
PpuMI RGGWCCY 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 717, 730
PspGI CCWGG 2 cut(s) 717, 730
PspN4I GGNNCC 6 cut(s) 410, 654, 723, 724, 933, 1041
PspPI GGNCC 4 cut(s) 350, 501, 722, 1151
PspPPI RGGWCCY 1 cut(s) 722
PstNI CAGNNNCTG 2 cut(s) 46, 299
PsuI RGATCY 2 cut(s) 745, 931
RsaI GTAC 2 cut(s) 572, 1015
RsaNI GTAC 2 cut(s) 571, 1014
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 639
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 495, 1161
SatI GCNGC 2 cut(s) 206, 209
Sau3AI GATC 2 cut(s) 745, 931
Sau96I GGNCC 4 cut(s) 350, 501, 722, 1151
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 719, 732
SduI GDGCHC 1 cut(s) 639
SfaNI GCATC 5 cut(s) 450, 795, 832, 1012, 1135
SfcI CTRYAG 2 cut(s) 741, 963
SfoI GGCGCC 1 cut(s) 410
Sfr274I CTCGAG 1 cut(s) 645
SinI GGWCC 1 cut(s) 722
SlaI CTCGAG 1 cut(s) 645
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 431, 645
SmoI CTYRAG 2 cut(s) 431, 645
SpeI ACTAGT 1 cut(s) 979
Sse9I AATT 3 cut(s) 246, 526, 765
SsiI CCGC 3 cut(s) 152, 362, 632
SspDI GGCGCC 1 cut(s) 408
SspI AATATT 1 cut(s) 661
SspMI CTAG 1 cut(s) 980
SstI GAGCTC 1 cut(s) 639
StyD4I CCNGG 2 cut(s) 717, 730
StyI CCWWGG 1 cut(s) 1125
TaqI TCGA 2 cut(s) 123, 646
TasI AATT 3 cut(s) 246, 526, 765
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 2 cut(s) 287, 686
Tru1I TTAA 2 cut(s) 495, 1161
Tru9I TTAA 2 cut(s) 495, 1161
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 2 cut(s) 205, 208
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 167, 416, 957
TspGWI ACGGA 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 722
XagI CCTNNNNNAGG 1 cut(s) 993
XapI RAATTY 1 cut(s) 765
XhoI CTCGAG 1 cut(s) 645
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.