Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004820

Physical Location & Seq

Genomic Coordinates Forward (+)

31744593 .. 31766067

Gene Structure 21475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1256700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGGCCGATCCTCTGGAGAGGCTGGGATCGGAGGCCGTAGGGTGCGAATCGAACATCAAGGACGAACTCTCCATGGAGATCGATTCTCCTTTCAAAGAAAACACCGCCACCGCCGACGACTGGAGAAAGGCGCTCAGCAAGGTCGTCCCCGCCGTCGTCGTTCTGCGGACAACCGCCTGTCGTGCTTTTGACACTGAGGCCGCTGGTGCTAGCTATGCCACCGGCTTCATCGTCGATAAGCGCCGCGGGATCATCCTCACCAATCGACATGTTGTCAAGCCTGGACCTGTAGTTGCAGAAGCTATGTTCGTGAACCGTGAAGAAATTCCAGTATATCCTATATACAGGGATCCAGTCCATGATTTTGGCTTCTTTTGTTATGATCCTAGTGCAGTACAATTTCTTAGTTATGAGGAGATTCCTCTGGCCCCTGAGGTTGCTTGTGTTGGACTAGAAATCAGGGTTGTTGGTAATGATAGTGGTGAGAAGGTCTCAATTTTGGCTGGTACTATTGCTCGTTTGGATAGAGATGCTCCACATTACAAAAAGGATGGTTATAATGACTTCAATACATTCTACATGCAAGCTGCATCTGGTACTAAAGGTGGTTCCAGTGGATCTCCTGTGATTGATTGGCTAGGCAGGGCGGTGGCCTTGAATGCTGGTAGCAAGTCATCAAGTGCTTCAGCTTTTTTCTTACCATTAGAACGAGTTGTGAGGGCATTGAACTATCTCCAGAAAGGCAGAGATTCATTTGTCAATAAATGGGAGGCAGTCTCTATACCTCGTGGTACGCTTCAGGTGACATTTGTCCATAAAGGGTTTGATGAGACACGCCGGCTTGGTCTTCAAAGTGAAACAGAGCAGTTAGTTCGTCGTGCATCTCCCGAGGGTGAAACTGGGATGCTGGTTGTTGACTCTGTGGTTCCAGGTGGTCCGGCGTACAAGTGTTTGGAGCCAGGTGATGTTCTTGTTTGCATGAATGGGAAAGTAATTACCCAATTCCTAAAATTGGAGACTTTACTTGATGACAGTGTCAATCAAAAGATTGAATTACAGATTGAAAGGGGTGGCACACAGTTAACTGTTAACTTGACGGTGCAAGATTTACACTCAATAACCCCTAATTACTTCTTGGAAGTAAGTGGTGGAGTGATACAACCTCTCTCGTACCAACAGCTTGTCGCTAGAAATTTTCGTTTCCCTTGTGGCCTTGTATATGTAACAGAACCAGGATATATGCTCCTTAGAGCTGGAGTTCCTCGCCATGCTATCATTAAGAAGTTTGCTGGTGAGGAAATATCACAACTTGAGGATCTAATCTCAGTTCTATGCAAGCTATCTCGAGGTGCTCGAGTGCCATTAGAGTATATAAGCTACATGGATCGTCATCGAAGGAAGTCAGTCCTGGTCACGGTTGATCGCCATGAATGGTATGCGCCTCCACAGATATACACTCGTGATGATTGTACTGGTCTTTGGACTGCTAAGCCTGTGTTTCAACATGATGGTAATCTACAATCATCTGGTGTCAATGAACTCGGAGGTACAGGAAGCCAAGCAGTTCCACTAAGTAGCGAAGTCACTTCCATGGTAGACATGCAGCATAATAGCCACGATCAATTGACCGATGGTGTCACCAGTATGGAAACCAGTTATGAGCATGTATCTGAAGGAGCACATTGTAGGGATGAACTGGATGTTGAAACAAAGAAAAGGCGAGTGAAGGAGGGTTTTCCTTCTGATGGAAATGTTGTTGCTGACTGTTCTTTTCCTGAAGCAAATGACAGTAAGCTGGATGATCCAAATACTATGGAGAATGCTGTTCTGAGAGACTTTGAAGGTGCAAATGTAGCAACTGCTAATGCTTCATTAGCTGAACGTGTTATAGAGCCGACTCTTGTTATGCTTGAGGTTCATGTGCCACCTTCATGTATGCTTGATGGTGTACATTCACAACATTTTTTCGGGACTGGTGTCATCATCTATCATTCTGATAACATGGGATTGGTTGCTGTTGACAAGAACACAGTTGCAATAGCTGCATCTGATGTGATGCTTTCATTTGCTGCTTTTCCAATTGAAATTCCTGGCGAGGTGGTTTTTCTCCACCCTGTTCACAATTATGCTCTCATTTCATATGATCCCTTGGCCCTGGGAGCTGTTGGTTCCTCTGCTGTTCGTGCTGCTGAACTACTGCCTGAGCCTGCATTGCGTAGGGGGGATTCTGTGTATCTTGTGGGGTTAAGTAGAAGCCTACAAGCAACATCTAGAAAGTCAATTGTAACTAACCCATGTGCTGCATTAAATATTGGATCTGCTGATTGTCCACGGTACAGGGCAACCAATATGGAGGTCATTGAGCTTGATACTGATTTTGGCAGTACATTTTCTGGTGTACTAACCGATGAGTATGGAAGGGTTCAAGCCATCTGGGGTAGCTTTTCAACTCAGCTAAAATTTGGTGGCAGTAGCTCCGAAGATCATCAGTTTGTTAGAGGTCTCCCTATTTATTCAATCAGTCAAGTCCTAGAAAAAATTATTTCTGGTGCTCAAGGCCCTCCTCTTCTCATAAATCGTGTCAAAAGGCCAATGCCTCTTGTGAGAATTTTAGAGGTTGAACTATATCCTACATTGCTTTCAAAAGCCCGAAGCTTTGGACTGAGTGATGATTGGGTCCAAGCTCTTGTTAAGAAAGATCCGATTAGACGACAAGTTTTACGTGTCAAAGGATGTTTAGCTGGATCTAAAGCCGAAAATCTGTTAGAACAAGGAGACATGGTTTTGGCGATCAATAAGGAGCCAGTTACATGTTTCTCCGATGTCGAAAATGTCTGCCAAGCACTAGATAAAAGTGAAAACAACAATGGCCAGCTTGACATGACCATTTTTCGCCAGGGGCGCGAAATCAATCTTCTTGTGGGAACGGATGTTAGGGATGGAAGTGGTTCTACTCGTGTAATAAATTGGTGTGGATGTATCGTTCAGGATCCTCATCCAGCAGTGCGTGCTCTTGGGTTTCTTCCAGATGAAGGTCATGGTGTATATGTCGCGAGGTGGTGTCATGGTAGTCCAGTGCATAGATATGGTCTATATGCTCTTCAGTGGATTGTTGAAGTCAACGGAAAAAAGACTCCTGATTTAGATTCTTTCGTGAATGTAACAAAGGAATTGGAACACGGACAGTTTGTTCGTGTGAGGACAGTCCATCTAAATGGGAAGCCAAGAGTTCTAACATTGAAACAGGACTTGCACTACTGGCCCACATGGGAGTTGAGATTTGATCCTGATTCTGCCATCTGGCGTCGAAAGACGATAAAGGCGCTGGATTATACCAGTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009642 GO:0009644 GO:0009892 GO:0010109 GO:0010205 GO:0016787 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0042548 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:1905156

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_1 PDZ_2 Trypsin_2

Protein Analysis

1115

Amino Acids

122.74

Weight (kDa)

5.65

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	73 - 219	7.5e-20	Trypsin-like peptidase domain
PDZ_2	PF13180	298 - 367	1.2e-06	PDZ domain
PDZ_6	PF17820	304 - 356	2.5e-08	PDZ domain
PDZ_1	PF12812	372 - 447	4.7e-11	PDZ-like domain
PDZ_1	PF12812	985 - 1059	2.7e-07	PDZ-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004820)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03380 AT3G03380
fragaria_vesca	FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890
malus_domestica	MD17G1256700.v1.1
prunus_persica	Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1
pyrus_communis	pycom17g25890
rosa_chinensis	RchiOBHm_Chr7g0220101
rosa_laevigata	RLG00000002258
rosa_multiflora	Rmu_sc0007553.1_g000006 Rmu_sc0018981.1_g000001
rosa_roxburghii	Rroxscaffold_3G00239790
rosa_rugosa	Rorug07G0188800 Rorug07G0188900 Rorug07G0188900 Rorug07G0189000
rosa_samantha	Rh7AG332500 Rh7BG322400 Rh7CG350000 Rh7DG328200
rosa_wichuraiana	Rw7G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	558
AccI	GTMKAC	1 cut(s)	1596
AccII	CGCG	3 cut(s)	246, 2904, 3053
AciI	CCGC	9 cut(s)	105, 111, 150, 166, 174, 201, 244, 246, 647
AcoI	YGGCCR	2 cut(s)	3, 2869
AcsI	RAATTY	5 cut(s)	324, 1192, 2085, 2459, 2607
AcuI	CTGAAG	5 cut(s)	669, 782, 1692, 1797, 3086
AcyI	GRCGYC	1 cut(s)	3304
AfiI	CCNNNNNNNGG	5 cut(s)	120, 1012, 1414, 1745, 3032
AflIII	ACRYGT	4 cut(s)	268, 1882, 2722, 2810
AhdI	GACNNNNNGTC	1 cut(s)	272
AjnI	CCWGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
AloI	GAACNNNNNNTCC	4 cut(s)	2967, 2999, 3174, 3206
Alw21I	GWGCWC	4 cut(s)	1354, 1681, 2554, 3013
Alw26I	GTCTC	7 cut(s)	496, 781, 824, 1010, 1827, 2507, 2769
Ama87I	CYCGRG	3 cut(s)	887, 1344, 1353
ApeKI	GCWGC	6 cut(s)	587, 1603, 2042, 2069, 2186, 2300
ApoI	RAATTY	5 cut(s)	324, 1192, 2085, 2459, 2607
ArsI	GACNNNNNNTTYG	2 cut(s)	3174, 3206
Asp700I	GAANNNNTTC	2 cut(s)	324, 2947
AspLEI	GCGC	5 cut(s)	133, 243, 1441, 2904, 3325
AspS9I	GGNCC	7 cut(s)	284, 427, 935, 2152, 2558, 2677, 3261
AsuHPI	GGTGA	7 cut(s)	250, 494, 814, 905, 974, 1304, 1630
AsuNHI	GCTAGC	1 cut(s)	209
AvaI	CYCGRG	3 cut(s)	887, 1344, 1353
AvaII	GGWCC	3 cut(s)	284, 935, 2677
AxyI	CCTNAGG	1 cut(s)	432
BalI	TGGCCA	1 cut(s)	2871
BamHI	GGATCC	2 cut(s)	349, 2989
BarI	GAAGNNNNNNTAC	2 cut(s)	2935, 2967
BauI	CACGAG	3 cut(s)	786, 1458, 2955
BbsI	GAAGAC	1 cut(s)	839
Bbv12I	GWGCWC	4 cut(s)	1354, 1681, 2554, 3013
BbvI	GCAGC	6 cut(s)	574, 1615, 2029, 2056, 2173, 2287
BccI	CCATC	9 cut(s)	545, 1502, 1625, 1739, 1937, 2438, 2933, 3216, 3305
BceAI	ACGGC	2 cut(s)	20, 137
BcgI	CGANNNNNNTGC	2 cut(s)	854, 888
BciT130I	CCWGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
BcoDI	GTCTC	7 cut(s)	496, 781, 824, 1010, 1827, 2507, 2769
BfaI	CTAG	8 cut(s)	210, 387, 452, 638, 1188, 2271, 2531, 2846
BfmI	CTRYAG	1 cut(s)	288
BfoI	RGCGCY	3 cut(s)	134, 244, 3326
BisI	GCNGC	8 cut(s)	201, 244, 588, 1604, 2043, 2070, 2187, 2301
BlpI	GCTNAGC	2 cut(s)	134, 1488
BlsI	GCNGC	8 cut(s)	202, 245, 589, 1605, 2044, 2071, 2188, 2302
Bme1390I	CCNGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
Bme18I	GGWCC	3 cut(s)	284, 935, 2677
BmeRI	GACNNNNNGTC	1 cut(s)	272
BmeT110I	CYCGRG	3 cut(s)	887, 1344, 1353
BmgT120I	GGNCC	7 cut(s)	284, 427, 935, 2152, 2558, 2677, 3261
BmiI	GGNNCC	9 cut(s)	351, 429, 610, 927, 957, 2170, 2678, 2802, 2991
BmrFI	CCNGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
BmrI	ACTGGG	1 cut(s)	909
BmsI	GCATC	6 cut(s)	520, 599, 890, 894, 2046, 2054
BmtI	GCTAGC	1 cut(s)	213
BmuI	ACTGGG	1 cut(s)	909
BoxI	GACNNNNGTC	2 cut(s)	809, 1976
BpiI	GAAGAC	1 cut(s)	839
BplI	GAGNNNNNCTC	4 cut(s)	476, 508, 761, 793
BpmI	CTGGAG	4 cut(s)	35, 142, 719, 1275
Bpu10I	CCTNAGC	1 cut(s)	2202
Bpu1102I	GCTNAGC	2 cut(s)	134, 1488
BpuEI	CTTGAG	3 cut(s)	1331, 1931, 2538
Bsa29I	ATCGAT	1 cut(s)	81
BsaAI	YACGTR	1 cut(s)	2723
BsaBI	GATNNNNATC	3 cut(s)	1389, 1512, 2994
BsaHI	GRCGYC	1 cut(s)	3304
BsaI	GGTCTC	2 cut(s)	496, 2507
BsaJI	CCNNGG	9 cut(s)	72, 244, 888, 1590, 2148, 2154, 2155, 2330, 2896
BsaXI	ACNNNNNCTCC	4 cut(s)	53, 83, 2766, 2796
Bsc4I	CCNNNNNNNGG	5 cut(s)	120, 1012, 1414, 1745, 3032
Bse118I	RCCGGY	2 cut(s)	221, 837
Bse21I	CCTNAGG	1 cut(s)	432
Bse3DI	GCAATG	2 cut(s)	2210, 2633
Bse8I	GATNNNNATC	3 cut(s)	1389, 1512, 2994
BseBI	CCWGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
BseCI	ATCGAT	1 cut(s)	81
BseDI	CCNNGG	9 cut(s)	72, 244, 888, 1590, 2148, 2154, 2155, 2330, 2896
BseJI	GATNNNNATC	3 cut(s)	1389, 1512, 2994
BseLI	CCNNNNNNNGG	5 cut(s)	120, 1012, 1414, 1745, 3032
BseMI	GCAATG	2 cut(s)	2210, 2633
BseMII	CTCAG	8 cut(s)	148, 186, 423, 1338, 1820, 2193, 2465, 2654
BseRI	GAGGAG	2 cut(s)	428, 2553
BseXI	GCAGC	6 cut(s)	574, 1615, 2029, 2056, 2173, 2287
BseYI	CCCAGC	1 cut(s)	22
BsgI	GTGCAG	1 cut(s)	411
Bsh1236I	CGCG	3 cut(s)	246, 2904, 3053
BshVI	ATCGAT	1 cut(s)	81
BsiHKAI	GWGCWC	4 cut(s)	1354, 1681, 2554, 3013
BsiHKCI	CYCGRG	3 cut(s)	887, 1344, 1353
BsiSI	CCGG	3 cut(s)	222, 838, 938
BslFI	GGGAC	2 cut(s)	131, 1984
BslI	CCNNNNNNNGG	5 cut(s)	120, 1012, 1414, 1745, 3032
BsmAI	GTCTC	7 cut(s)	496, 781, 824, 1010, 1827, 2507, 2769
BsmFI	GGGAC	2 cut(s)	131, 1984
BsmI	GAATGC	2 cut(s)	664, 1825
Bso31I	GGTCTC	2 cut(s)	496, 2507
BsoBI	CYCGRG	3 cut(s)	887, 1344, 1353
Bsp1286I	GDGCHC	4 cut(s)	1354, 1681, 2554, 3013
Bsp1407I	TGTACA	1 cut(s)	1948
Bsp1720I	GCTNAGC	2 cut(s)	134, 1488
Bsp19I	CCATGG	2 cut(s)	72, 1590
Bsp68I	TCGCGA	1 cut(s)	3053
BspACI	CCGC	9 cut(s)	105, 111, 150, 166, 174, 201, 244, 246, 647
BspCNI	CTCAG	8 cut(s)	147, 187, 424, 1337, 1821, 2194, 2464, 2655
BspDI	ATCGAT	1 cut(s)	81
BspFNI	CGCG	3 cut(s)	246, 2904, 3053
BspLI	GGNNCC	9 cut(s)	351, 429, 610, 927, 957, 2170, 2678, 2802, 2991
BspOI	GCTAGC	1 cut(s)	213
BspQI	GCTCTTC	1 cut(s)	3105
BspTNI	GGTCTC	2 cut(s)	496, 2507
BsrDI	GCAATG	2 cut(s)	2210, 2633
BsrFI	RCCGGY	2 cut(s)	221, 837
BsrGI	TGTACA	1 cut(s)	1948
BssAI	RCCGGY	2 cut(s)	221, 837
BssECI	CCNNGG	9 cut(s)	72, 244, 888, 1590, 2148, 2154, 2155, 2330, 2896
BssNI	GRCGYC	1 cut(s)	3304
BssSI	CACGAG	3 cut(s)	786, 1458, 2955
BssT1I	CCWWGG	3 cut(s)	72, 1590, 2148
Bst2BI	CACGAG	3 cut(s)	786, 1458, 2955
Bst2UI	CCWGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
Bst6I	CTCTTC	2 cut(s)	2571, 3105
BstACI	GRCGYC	1 cut(s)	3304
BstAPI	GCANNNNNTGC	2 cut(s)	2042, 3008
BstAUI	TGTACA	1 cut(s)	1948
BstBAI	YACGTR	1 cut(s)	2723
BstC8I	GCNNGC	7 cut(s)	211, 585, 839, 1337, 2208, 2873, 3009
BstDSI	CCRYGG	4 cut(s)	72, 244, 1590, 2330
BstFNI	CGCG	3 cut(s)	246, 2904, 3053
BstH2I	RGCGCY	3 cut(s)	134, 244, 3326
BstHHI	GCGC	5 cut(s)	133, 243, 1441, 2904, 3325
BstMAI	GTCTC	7 cut(s)	496, 781, 824, 1010, 1827, 2507, 2769
BstNI	CCWGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
BstNSI	RCATGY	5 cut(s)	272, 583, 1603, 1667, 2814
BstPAI	GACNNNNGTC	2 cut(s)	809, 1976
BstSCI	CCNGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
BstSFI	CTRYAG	1 cut(s)	288
BstUI	CGCG	3 cut(s)	246, 2904, 3053
BstV1I	GCAGC	6 cut(s)	574, 1615, 2029, 2056, 2173, 2287
BstV2I	GAAGAC	1 cut(s)	839
BstX2I	RGATCY	7 cut(s)	349, 617, 1315, 2315, 2698, 2744, 2989
BstXI	CCANNNNNNTGG	2 cut(s)	365, 3215
BstYI	RGATCY	7 cut(s)	349, 617, 1315, 2315, 2698, 2744, 2989
Bsu15I	ATCGAT	1 cut(s)	81
Bsu36I	CCTNAGG	1 cut(s)	432
BsuTUI	ATCGAT	1 cut(s)	81
BtgI	CCRYGG	4 cut(s)	72, 244, 1590, 2330
BtsI	GCAGTG	1 cut(s)	3009
BtsIMutI	CAGTG	6 cut(s)	192, 619, 1039, 3009, 3081, 3110
BtuMI	TCGCGA	1 cut(s)	3053
Cac8I	GCNNGC	7 cut(s)	211, 585, 839, 1337, 2208, 2873, 3009
CfoI	GCGC	5 cut(s)	133, 243, 1441, 2904, 3325
Cfr10I	RCCGGY	2 cut(s)	221, 837
Cfr13I	GGNCC	7 cut(s)	284, 427, 935, 2152, 2558, 2677, 3261
Cfr42I	CCGCGG	1 cut(s)	247
ClaI	ATCGAT	1 cut(s)	81
CseI	GACGC	1 cut(s)	3293
DriI	GACNNNNNGTC	1 cut(s)	272
EaeI	YGGCCR	2 cut(s)	3, 2869
Eam1104I	CTCTTC	2 cut(s)	2571, 3105
Eam1105I	GACNNNNNGTC	1 cut(s)	272
EarI	CTCTTC	2 cut(s)	2571, 3105
Eco130I	CCWWGG	3 cut(s)	72, 1590, 2148
Eco31I	GGTCTC	2 cut(s)	496, 2507
Eco47I	GGWCC	3 cut(s)	284, 935, 2677
Eco57I	CTGAAG	5 cut(s)	669, 782, 1692, 1797, 3086
Eco81I	CCTNAGG	1 cut(s)	432
Eco88I	CYCGRG	3 cut(s)	887, 1344, 1353
EcoO109I	RGGNCCY	1 cut(s)	2558
EcoRII	CCWGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
EcoT14I	CCWWGG	3 cut(s)	72, 1590, 2148
ErhI	CCWWGG	3 cut(s)	72, 1590, 2148
FaqI	GGGAC	2 cut(s)	131, 1984
FauI	CCCGC	2 cut(s)	157, 239
FauNDI	CATATG	1 cut(s)	2140
FblI	GTMKAC	1 cut(s)	1596
Fnu4HI	GCNGC	8 cut(s)	201, 244, 588, 1604, 2043, 2070, 2187, 2301
Fsp4HI	GCNGC	8 cut(s)	201, 244, 588, 1604, 2043, 2070, 2187, 2301
FspBI	CTAG	8 cut(s)	210, 387, 452, 638, 1188, 2271, 2531, 2846
GlaI	GCGC	5 cut(s)	132, 242, 1440, 2903, 3324
GluI	GCNGC	8 cut(s)	201, 244, 588, 1604, 2043, 2070, 2187, 2301
GsaI	CCCAGC	1 cut(s)	26
GsuI	CTGGAG	4 cut(s)	35, 142, 719, 1275
HaeII	RGCGCY	3 cut(s)	134, 244, 3326
HapII	CCGG	3 cut(s)	222, 838, 938
HgaI	GACGC	1 cut(s)	3293
HhaI	GCGC	5 cut(s)	133, 243, 1441, 2904, 3325
Hin1I	GRCGYC	1 cut(s)	3304
Hin6I	GCGC	5 cut(s)	131, 241, 1439, 2902, 3323
HinP1I	GCGC	5 cut(s)	131, 241, 1439, 2902, 3323
HincII	GTYRAC	5 cut(s)	916, 1083, 1090, 2020, 3121
HindII	GTYRAC	5 cut(s)	916, 1083, 1090, 2020, 3121
HindIII	AAGCTT	1 cut(s)	2653
HpaI	GTTAAC	2 cut(s)	1083, 1090
HpaII	CCGG	3 cut(s)	222, 838, 938
HphI	GGTGA	7 cut(s)	250, 494, 814, 905, 974, 1304, 1630
Hpy99I	CGWCG	6 cut(s)	119, 158, 161, 236, 879, 3309
HpyAV	CCTTC	9 cut(s)	481, 1389, 1667, 1720, 1749, 1835, 1938, 2412, 3026
HpyCH4IV	ACGT	2 cut(s)	1882, 2722
HpySE526I	ACGT	2 cut(s)	1882, 2722
Hsp92I	GRCGYC	1 cut(s)	3304
HspAI	GCGC	5 cut(s)	131, 241, 1439, 2902, 3323
KroI	GCCGGC	1 cut(s)	837
KroNI	GCCGGC	1 cut(s)	839
KspAI	GTTAAC	2 cut(s)	1083, 1090
KspI	CCGCGG	1 cut(s)	247
LguI	GCTCTTC	1 cut(s)	3105
LmnI	GCTCC	7 cut(s)	538, 955, 1248, 1676, 2159, 2480, 2800
Lsp1109I	GCAGC	6 cut(s)	574, 1615, 2029, 2056, 2173, 2287
LweI	GCATC	6 cut(s)	520, 599, 890, 894, 2046, 2054
MaeI	CTAG	8 cut(s)	210, 387, 452, 638, 1188, 2271, 2531, 2846
MaeII	ACGT	2 cut(s)	1882, 2722
MaeIII	GTNAC	8 cut(s)	802, 1222, 1411, 1582, 1636, 2284, 2806, 3160
MboII	GAAGA	7 cut(s)	332, 839, 2492, 2558, 2906, 3014, 3092
MfeI	CAATTG	3 cut(s)	1622, 2079, 2280
MflI	RGATCY	7 cut(s)	349, 617, 1315, 2315, 2698, 2744, 2989
MhlI	GDGCHC	4 cut(s)	1354, 1681, 2554, 3013
MlsI	TGGCCA	1 cut(s)	2871
MluNI	TGGCCA	1 cut(s)	2871
MlyI	GAGTC	3 cut(s)	911, 1891, 3127
MmeI	TCCRAC	1 cut(s)	427
Mox20I	TGGCCA	1 cut(s)	2871
MroNI	GCCGGC	1 cut(s)	837
MroXI	GAANNNNTTC	2 cut(s)	324, 2947
MscI	TGGCCA	1 cut(s)	2871
MseI	TTAA	6 cut(s)	1082, 1089, 1278, 2246, 2306, 2691
MslI	CAYNNNNRTG	6 cut(s)	1589, 1643, 1930, 2124, 3084, 3213
Msp20I	TGGCCA	1 cut(s)	2871
MspA1I	CMGCKG	2 cut(s)	203, 246
MspI	CCGG	3 cut(s)	222, 838, 938
MspR9I	CCNGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
MunI	CAATTG	3 cut(s)	1622, 2079, 2280
Mva1269I	GAATGC	2 cut(s)	664, 1825
MvaI	CCWGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
MvnI	CGCG	3 cut(s)	246, 2904, 3053
NaeI	GCCGGC	1 cut(s)	839
NcoI	CCATGG	2 cut(s)	72, 1590
NdeI	CATATG	1 cut(s)	2140
NgoMIV	GCCGGC	1 cut(s)	837
NheI	GCTAGC	1 cut(s)	209
NlaIV	GGNNCC	9 cut(s)	351, 429, 610, 927, 957, 2170, 2678, 2802, 2991
NmuCI	GTSAC	4 cut(s)	802, 1411, 1582, 1636
NruI	TCGCGA	1 cut(s)	3053
NspI	RCATGY	5 cut(s)	272, 583, 1603, 1667, 2814
PaeR7I	CTCGAG	2 cut(s)	1344, 1353
PasI	CCCWGGG	1 cut(s)	2155
PciI	ACATGT	2 cut(s)	268, 2810
PciSI	GCTCTTC	1 cut(s)	3105
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 1351
PctI	GAATGC	2 cut(s)	664, 1825
PdiI	GCCGGC	1 cut(s)	839
PdmI	GAANNNNTTC	2 cut(s)	324, 2947
PfeI	GAWTC	7 cut(s)	47, 83, 418, 749, 2225, 3146, 3290
PflFI	GACNNNGTC	1 cut(s)	1034
PkrI	GCNGC	8 cut(s)	202, 245, 589, 1605, 2044, 2071, 2188, 2302
PleI	GAGTC	3 cut(s)	911, 1891, 3127
PpsI	GAGTC	3 cut(s)	911, 1891, 3127
Ppu21I	YACGTR	1 cut(s)	2723
PscI	ACATGT	2 cut(s)	268, 2810
PshAI	GACNNNNGTC	2 cut(s)	809, 1976
PsiI	TTATAA	1 cut(s)	558
Psp6I	CCWGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
PspFI	CCCAGC	1 cut(s)	22
PspGI	CCWGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
PspN4I	GGNNCC	9 cut(s)	351, 429, 610, 927, 957, 2170, 2678, 2802, 2991
PspPI	GGNCC	7 cut(s)	284, 427, 935, 2152, 2558, 2677, 3261
PspXI	VCTCGAGB	1 cut(s)	1353
PsuI	RGATCY	7 cut(s)	349, 617, 1315, 2315, 2698, 2744, 2989
PsyI	GACNNNGTC	1 cut(s)	1034
RruI	TCGCGA	1 cut(s)	3053
RseI	CAYNNNNRTG	6 cut(s)	1589, 1643, 1930, 2124, 3084, 3213
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	3105
SaqAI	TTAA	6 cut(s)	1082, 1089, 1278, 2246, 2306, 2691
SatI	GCNGC	8 cut(s)	201, 244, 588, 1604, 2043, 2070, 2187, 2301
Sau96I	GGNCC	7 cut(s)	284, 427, 935, 2152, 2558, 2677, 3261
SchI	GAGTC	3 cut(s)	911, 1891, 3127
ScrFI	CCNGG	8 cut(s)	282, 930, 960, 1233, 1409, 2091, 2156, 2897
SduI	GDGCHC	4 cut(s)	1354, 1681, 2554, 3013
SfaNI	GCATC	6 cut(s)	520, 599, 890, 894, 2046, 2054
SfcI	CTRYAG	1 cut(s)	288
Sfr274I	CTCGAG	2 cut(s)	1344, 1353
Sfr303I	CCGCGG	1 cut(s)	247
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	3 cut(s)	284, 935, 2677
SlaI	CTCGAG	2 cut(s)	1344, 1353
SmiMI	CAYNNNNRTG	6 cut(s)	1589, 1643, 1930, 2124, 3084, 3213
SmlI	CTYRAG	5 cut(s)	1310, 1344, 1353, 1910, 2553
SmoI	CTYRAG	5 cut(s)	1310, 1344, 1353, 1910, 2553
SsiI	CCGC	9 cut(s)	105, 111, 150, 166, 174, 201, 244, 246, 647
SspI	AATATT	1 cut(s)	2311
SspMI	CTAG	8 cut(s)	210, 387, 452, 638, 1188, 2271, 2531, 2846
StyD4I	CCNGG	8 cut(s)	280, 928, 958, 1231, 1407, 2089, 2154, 2895
StyI	CCWWGG	3 cut(s)	72, 1590, 2148
TaiI	ACGT	2 cut(s)	1885, 2725
TaqI	TCGA	9 cut(s)	50, 81, 234, 265, 1345, 1354, 1393, 2826, 3307
TaqII	GACCGA	1 cut(s)	1643
TatI	WGTACW	5 cut(s)	394, 1469, 1948, 2384, 2398
TauI	GCSGC	2 cut(s)	203, 246
TfiI	GAWTC	7 cut(s)	47, 83, 418, 749, 2225, 3146, 3290
Tru1I	TTAA	6 cut(s)	1082, 1089, 1278, 2246, 2306, 2691
Tru9I	TTAA	6 cut(s)	1082, 1089, 1278, 2246, 2306, 2691
TscAI	CASTG	6 cut(s)	199, 619, 1039, 3009, 3081, 3110
TseFI	GTSAC	4 cut(s)	802, 1411, 1582, 1636
TseI	GCWGC	6 cut(s)	587, 1603, 2042, 2069, 2186, 2300
Tsp45I	GTSAC	4 cut(s)	802, 1411, 1582, 1636
TspGWI	ACGGA	3 cut(s)	2942, 3138, 3195
TspRI	CASTG	6 cut(s)	199, 619, 1039, 3009, 3081, 3110
Tth111I	GACNNNGTC	1 cut(s)	1034
VpaK11BI	GGWCC	3 cut(s)	284, 935, 2677
XapI	RAATTY	5 cut(s)	324, 1192, 2085, 2459, 2607
XbaI	TCTAGA	1 cut(s)	2270
XceI	RCATGY	5 cut(s)	272, 583, 1603, 1667, 2814
XhoI	CTCGAG	2 cut(s)	1344, 1353
XmiI	GTMKAC	1 cut(s)	1596
XmnI	GAANNNNTTC	2 cut(s)	324, 2947
XspI	CTAG	8 cut(s)	210, 387, 452, 638, 1188, 2271, 2531, 2846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.