Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0004820

Physical Location & Seq

Genomic Coordinates Forward (+)

39753035 .. 39777736

Gene Structure 24702 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG328200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3330 bp

ATGGGCGATCCTCTGGACAGACTTGAATCAGAGGACGCCGGAGGATCGAGCCTGAAGGACATGGAGATCGACCCGCCTTTCAGAGAAAGCACCGCCACGACCGAGGACTGGCGGAAGGCGCTTGCCAACGTCGTCCCCGCCGTCGTCGTTCTGAGGACCACGGCGTGTCGTGCCTTCGATACTGAAGCCGCCGGTGCTAGCTATGCCACCGGCTTTGTCGTCGATAAGCGCCGCGGGATCATCCTCACCAACCGCCATGTTGTCAAGCCTGGACCTGTAGTTGCGGAAGCTATGTTTGTCAATCGCGAAGAAGTTCCAGTATATCCTATCTATAGGGATCCGGTTCATGATTTTGGCTTCTTTTGTTATGATCCTGGTGCAATACAGTTTCTGAAGTATGAAGAGATTCCTCTTGCCCCTGAGGCTGCTTCTGTTGGATTAGAAATCAGGGTTGTTGGTAACGATAGTGGTGAGAAGGTCTCCATTTTGGCTGGTACTCTTGCCCGTTTAGATAGAGATGCTCCTTATTACAAAAAGGATGGCTATAATGATTTCAATACATTCTACATGCAAGCTGCATCTGGTACCAAAGGTGGGTCTAGTGGCTCTCCGGTGATTGATTGGCTAGGTAGGGCAGTGGCTTTGAATGCTGGTAGCAAGTCATCAAGTGCATCAGCATTTTTTTTACCATTGGAACGAGTTGTGAGGGCATTGAAGTTTCTCCAAATAGGCAGAGATTCTTTCGTCAAAAAATGGGAGGCAGTTTCTATACTGCGTGGTACGCTTCAGGTAACTTTTGTCCACAAAGGATTTGATGAGACACGGCGACTTGGTCTTCAAAGTGAGACGGAGCAGAGGGTTCGACATGCGTCTCCGCAGGGTGAAACTGGAATGCTTGTTGTTGAATCTATGGTGCCAGGCGGTCCAGCTCATAAGTGTTTGGAGCCAGGTGATGTTCTTGTTTGCATGAATGGGGAAGTAATTACCCAATTTCTAAAAATGGAGACATTACTTGATGATAGTGTGAATCAAAAAATTGAAATACAGATCGAAAGGGGTGGCACACCATTTACTGTGAACTTGCTGGTGCAGGATTTGCACTCAATAACCCCTAATTACTTCTTAGAAGTAAGTGGTGCAGTGATACACCCTCTCTCGTATCAACAGGCTAGAAATTTTCGCTTCGAGTGTGGCCTTGTGTATGTGGCAGAGCCAGGATATATGCTCTTTAGAGCTGGAGTTCCTCGTCATGCTATCATTAAGAAGTTTGCTGGTCAGGAAATATCACTACTTGAGGATCTAATTTCAGTGCTATGCAAGCTATCTCGGGGTGCTCGAGTGCCGTTAGAGTATATAAGCTATGCAGATCGTCATCGGAGGAAGTCAGTCCTGGTCACCGTTGATCGCCATGAATGGTATGCCCCTCCACAGATATACACTCGTGATGATAGTACTGGTCTTTGGACGGTCAAGCCTGCTTTTCAGCCTGATGCTGGTCTACTTTCATCTGGTATCAATGATCTTGGTGGTATAAGAAGTGATGCAGTTCCTCTATGTACTGAGGCCTCTTCTGTTGGGCACATGCATCGTAATAGCCATGCAGAATTGAATGAAGGGGTCACCAGTATGGAAACCAGTTATGAACATTCTTCTGAAGGAGGACATCCTCGGGCTGAATCTGATGTTGAAACAAAGAAACGCCGAGTGAAGGTGGATTTTTCTTCTGATGGAAATGTCGTAGCCGACGGTTCCTTTCTTGAAACAGATGAAGGTGATCCAGAGGATGCAAATACTGTGGAGAATTCTGTTATGAGAGATCTTCAAGGTGAAAATGTAGCAGCAGCTAATTCTTCACTAGCTGAACGTATTATCGAGCCAACTCTTGTTATGCTTGAGGTTCATGTGCCACCATCATGTATGCTCGATGGTGTACATTCACAACATTTTTTTGGGACTGGTGTTATTATATACCACTCTCAAAATATGGGACTGATTGTCGTTGACAAGAATACAGTTGCGGTATCTGCATCTGATGTGATGCTCTCATTCGCCGCATTTCCAATTGAAATTCCAGGGGAGGTGGTTTTTCTCCATCCCGTTCACAATTATGCTCTCATTTCATATGATCCATTGGCTCTGGGCGCTGTTGGTATCTCGCTTGTTCGGGCTGCGAAACTACTTCCTGAGCCTGCAATACGTCGTGGGGATTCTGTGCATCTGGTTGGGCTAAGTAGAAGCCTACAAGCAACATCTAGAAAGTCTATTGTGACCAACCCATGCGCTGCAGTAAATATTAGCTCTGCTGATTGTCCACGTTACAGAGCAACCAATATGGAAGTTATCGAGCTTGATACTGATTTTGGCAGTACATTCTCTGGAGTGCTAACTGATGAGCATGGAAGGGTCCGAGCTATTTGGGGTAGCTTTTCAACTCAGTTGAAATTTGGTTGCAGTAGCTCCGAAGATCATCAATTTGTAAGAGGTATCCCAATATATACAATCAGTCAAGTCCTTGAACAAATCATATGTGGTGCTCAAGGCCCTCCTCTTCTCATAAATTGTGTCAAAAGGACGATGCCTCTAGTTAGAATTTTGGAGGTTGAACTATATCCTACACTGCTTTCAAAAGCCCGCAGTTTTGGACTGAGTGATGATTGGGTCCAAGCACTTGTCAAGAAAGATCCGATTAGACGACAGGTTTTACGTGTGAAAGGGTGTTTAGCTGGATCTAAAGCCGAAAATCTGTTAGAACAAGGAGACATGGTCTTGGCAATCAATAAGGAACCAGTTACATGCTTCCGTGATGTAGAAAATGCCTGTCAAGAATTGGGAAAACATGAAAACAAAGATGGAAAGCTTAACATGACCATATTCCGTCAGGGGCATGAGATTGATCTTCTCGTCGAAACAGATATTAGAGATGGGAGTGGTACTACACATGTAATAAATTGGTGTGGATGTATTGTTCAGGATCCCCATCCCGCAGTGCGTGCTCTTGGATTTCTTCCGGAAGAAGGTCATGGTGTTTATGTGGCAAGGTGGTGTCATGGTAGCCCAGCGCATAGATATGGACTGTATGCTCTTCAGTGGATTGTTGAAGTTAATGGAAAACAGATTCCTGATTTAGATGCTTTTTTGAATGTCGCAAAGGAAATAGAACATGGAGAGTTTGTTCGTGTACGGACCATCCACTTAAATGGAAAACCTAAAGTTTTAACGTTGAAGCAGGACTTGCACTACTGGCCTACATGGGAGGTCAGATTTGATCCTGATTCTGCTATATGGCATCGAAACACAATTAAGGCACTAGACTATAGTTCTAATCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009642 GO:0009644 GO:0009892 GO:0010109 GO:0010205 GO:0016787 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0042548 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:1905156

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_1 PDZ_2 Trypsin_2

Protein Analysis

1109

Amino Acids

122.26

Weight (kDa)

5.74

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	69 - 215	8.7e-21	Trypsin-like peptidase domain
PDZ_6	PF17820	300 - 352	1.5e-06	PDZ domain
PDZ_1	PF12812	368 - 441	1.7e-14	PDZ-like domain
PDZ_1	PF12812	979 - 1053	1.3e-06	PDZ-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004820)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03380 AT3G03380
fragaria_vesca	FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890
malus_domestica	MD17G1256700.v1.1
prunus_persica	Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1
pyrus_communis	pycom17g25890
rosa_chinensis	RchiOBHm_Chr7g0220101
rosa_laevigata	RLG00000002258
rosa_multiflora	Rmu_sc0007553.1_g000006 Rmu_sc0018981.1_g000001
rosa_roxburghii	Rroxscaffold_3G00239790
rosa_rugosa	Rorug07G0188800 Rorug07G0188900 Rorug07G0188900 Rorug07G0189000
rosa_samantha	Rh7AG332500 Rh7BG322400 Rh7CG350000 Rh7DG328200
rosa_wichuraiana	Rw7G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	584
AccB1I	GGYRCC	2 cut(s)	584, 913
AccI	GTMKAC	1 cut(s)	1498
AccII	CGCG	2 cut(s)	234, 306
AccIII	TCCGGA	1 cut(s)	3007
AclI	AACGTT	1 cut(s)	3218
AcsI	RAATTY	5 cut(s)	1174, 1801, 2067, 2441, 2589
AcuI	CTGAAG	6 cut(s)	74, 204, 413, 770, 1674, 3068
AcyI	GRCGYC	1 cut(s)	36
AdeI	CACNNNGTG	1 cut(s)	165
AfaI	GTAC	9 cut(s)	496, 586, 781, 1453, 1558, 1932, 2368, 2932, 3180
AfiI	CCNNNNNNNGG	5 cut(s)	108, 594, 1493, 1708, 3014
AflIII	ACRYGT	2 cut(s)	2704, 2938
AhdI	GACNNNNNGTC	1 cut(s)	1994
AjnI	CCWGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
AloI	GAACNNNNNNTCC	4 cut(s)	2949, 2981, 3156, 3188
Alw21I	GWGCWC	3 cut(s)	1336, 2536, 2995
Alw26I	GTCTC	6 cut(s)	484, 812, 839, 876, 998, 2751
AlwNI	CAGNNNCTG	1 cut(s)	391
Ama87I	CYCGRG	3 cut(s)	1326, 1335, 1668
Aor13HI	TCCGGA	1 cut(s)	3007
AoxI	GGCC	4 cut(s)	1192, 1563, 2539, 3242
ApeKI	GCWGC	6 cut(s)	425, 575, 1838, 1841, 2168, 2282
ApoI	RAATTY	5 cut(s)	1174, 1801, 2067, 2441, 2589
Asp700I	GAANNNNTTC	2 cut(s)	312, 405
Asp718I	GGTACC	1 cut(s)	584
AspLEI	GCGC	5 cut(s)	121, 231, 2144, 2282, 3061
AspS9I	GGNCC	7 cut(s)	156, 272, 923, 2404, 2540, 2659, 3183
AsuHPI	GGTGA	9 cut(s)	238, 482, 625, 893, 962, 1387, 1612, 1784, 1838
AsuNHI	GCTAGC	1 cut(s)	197
AvaI	CYCGRG	3 cut(s)	1326, 1335, 1668
AvaII	GGWCC	6 cut(s)	156, 272, 923, 2404, 2659, 3183
AxyI	CCTNAGG	1 cut(s)	420
BaeGI	GKGCMC	1 cut(s)	1581
BamHI	GGATCC	2 cut(s)	337, 2971
BanI	GGYRCC	2 cut(s)	584, 913
BauI	CACGAG	1 cut(s)	1440
BbsI	GAAGAC	1 cut(s)	827
Bbv12I	GWGCWC	3 cut(s)	1336, 2536, 2995
BbvI	GCAGC	6 cut(s)	412, 562, 1850, 1853, 2155, 2269
BccI	CCATC	9 cut(s)	533, 1721, 1918, 1919, 2100, 2843, 2915, 2985, 3194
BceAI	ACGGC	4 cut(s)	125, 177, 839, 1327
BcgI	CGANNNNNNTGC	4 cut(s)	842, 876, 1568, 1602
BciT130I	CCWGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
BciVI	GTATCC	1 cut(s)	2495
BcoDI	GTCTC	6 cut(s)	484, 812, 839, 876, 998, 2751
BfaI	CTAG	8 cut(s)	198, 600, 626, 1170, 1856, 2253, 2582, 3308
BfmI	CTRYAG	4 cut(s)	276, 331, 2283, 3313
BfoI	RGCGCY	3 cut(s)	122, 232, 2145
BfuI	GTATCC	1 cut(s)	2495
BglI	GCCNNNNNGGC	1 cut(s)	422
BglII	AGATCT	1 cut(s)	1816
BisI	GCNGC	9 cut(s)	189, 232, 426, 576, 1839, 1842, 2052, 2169, 2283
BlsI	GCNGC	9 cut(s)	190, 233, 427, 577, 1840, 1843, 2053, 2170, 2284
BmcAI	AGTACT	1 cut(s)	1453
Bme1390I	CCNGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
Bme18I	GGWCC	6 cut(s)	156, 272, 923, 2404, 2659, 3183
BmeRI	GACNNNNNGTC	1 cut(s)	1994
BmeT110I	CYCGRG	3 cut(s)	1326, 1335, 1668
BmgT120I	GGNCC	7 cut(s)	156, 272, 923, 2404, 2540, 2659, 3183
BmiI	GGNNCC	9 cut(s)	339, 586, 915, 945, 1750, 2405, 2660, 2784, 2973
BmrFI	CCNGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
BmtI	GCTAGC	1 cut(s)	201
BoxI	GACNNNNGTC	1 cut(s)	868
BpiI	GAAGAC	1 cut(s)	827
BplI	GAGNNNNNCTC	2 cut(s)	464, 496
BpmI	CTGGAG	2 cut(s)	1257, 2397
Bpu10I	CCTNAGC	1 cut(s)	2184
BpuEI	CTTGAG	3 cut(s)	1313, 1913, 2520
BsaAI	YACGTR	1 cut(s)	2705
BsaBI	GATNNNNATC	2 cut(s)	1371, 2976
BsaHI	GRCGYC	1 cut(s)	36
BsaI	GGTCTC	1 cut(s)	484
BsaJI	CCNNGG	5 cut(s)	102, 159, 232, 1667, 2072
BsaWI	WCCGGW	3 cut(s)	340, 610, 3007
BsaXI	ACNNNNNCTCC	4 cut(s)	2748, 2778, 3156, 3186
Bsc4I	CCNNNNNNNGG	5 cut(s)	108, 594, 1493, 1708, 3014
Bse118I	RCCGGY	2 cut(s)	191, 209
Bse1I	ACTGG	9 cut(s)	113, 317, 892, 1459, 1623, 1635, 1960, 2786, 3245
Bse21I	CCTNAGG	1 cut(s)	420
Bse8I	GATNNNNATC	2 cut(s)	1371, 2976
BseAI	TCCGGA	1 cut(s)	3007
BseBI	CCWGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
BseDI	CCNNGG	5 cut(s)	102, 159, 232, 1667, 2072
BseGI	GGATG	8 cut(s)	240, 544, 1663, 1789, 2092, 2963, 2977, 3186
BseJI	GATNNNNATC	2 cut(s)	1371, 2976
BseLI	CCNNNNNNNGG	5 cut(s)	108, 594, 1493, 1708, 3014
BseMII	CTCAG	6 cut(s)	143, 411, 1551, 2175, 2447, 2636
BseNI	ACTGG	9 cut(s)	113, 317, 892, 1459, 1623, 1635, 1960, 2786, 3245
BseRI	GAGGAG	1 cut(s)	2535
BseSI	GKGCMC	1 cut(s)	1581
BseXI	GCAGC	6 cut(s)	412, 562, 1850, 1853, 2155, 2269
BseYI	CCCAGC	1 cut(s)	3055
BsgI	GTGCAG	2 cut(s)	1109, 1158
Bsh1236I	CGCG	2 cut(s)	234, 306
Bsh1285I	CGRYCG	1 cut(s)	102
BshFI	GGCC	4 cut(s)	1194, 1565, 2541, 3244
BshNI	GGYRCC	2 cut(s)	584, 913
BsiEI	CGRYCG	1 cut(s)	102
BsiHKAI	GWGCWC	3 cut(s)	1336, 2536, 2995
BsiHKCI	CYCGRG	3 cut(s)	1326, 1335, 1668
BsiSI	CCGG	6 cut(s)	39, 192, 210, 341, 611, 3008
BslFI	GGGAC	3 cut(s)	119, 1966, 2001
BslI	CCNNNNNNNGG	5 cut(s)	108, 594, 1493, 1708, 3014
BsmAI	GTCTC	6 cut(s)	484, 812, 839, 876, 998, 2751
BsmBI	CGTCTC	2 cut(s)	839, 876
BsmFI	GGGAC	3 cut(s)	119, 1966, 2001
BsmI	GAATGC	2 cut(s)	652, 897
BsnI	GGCC	4 cut(s)	1194, 1565, 2541, 3244
Bso31I	GGTCTC	1 cut(s)	484
BsoBI	CYCGRG	3 cut(s)	1326, 1335, 1668
Bsp1286I	GDGCHC	4 cut(s)	1336, 1581, 2536, 2995
Bsp13I	TCCGGA	1 cut(s)	3007
Bsp1407I	TGTACA	1 cut(s)	1930
Bsp68I	TCGCGA	1 cut(s)	306
BspANI	GGCC	4 cut(s)	1194, 1565, 2541, 3244
BspCNI	CTCAG	6 cut(s)	144, 412, 1552, 2176, 2446, 2637
BspEI	TCCGGA	1 cut(s)	3007
BspFNI	CGCG	2 cut(s)	234, 306
BspHI	TCATGA	1 cut(s)	346
BspLI	GGNNCC	9 cut(s)	339, 586, 915, 945, 1750, 2405, 2660, 2784, 2973
BspMAI	CTGCAG	1 cut(s)	2287
BspOI	GCTAGC	1 cut(s)	201
BspQI	GCTCTTC	1 cut(s)	3087
BspT107I	GGYRCC	2 cut(s)	584, 913
BspTNI	GGTCTC	1 cut(s)	484
BsrFI	RCCGGY	2 cut(s)	191, 209
BsrGI	TGTACA	1 cut(s)	1930
BsrI	ACTGG	9 cut(s)	113, 317, 892, 1459, 1623, 1635, 1960, 2786, 3245
BssAI	RCCGGY	2 cut(s)	191, 209
BssECI	CCNNGG	5 cut(s)	102, 159, 232, 1667, 2072
BssNI	GRCGYC	1 cut(s)	36
BssSI	CACGAG	1 cut(s)	1440
Bst2BI	CACGAG	1 cut(s)	1440
Bst2UI	CCWGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
Bst4CI	ACNGT	8 cut(s)	387, 1075, 1399, 1468, 1748, 1795, 2014, 3075
Bst6I	CTCTTC	4 cut(s)	396, 1573, 2553, 3087
BstACI	GRCGYC	1 cut(s)	36
BstAPI	GCANNNNNTGC	3 cut(s)	1096, 2990, 3232
BstAUI	TGTACA	1 cut(s)	1930
BstBAI	YACGTR	1 cut(s)	2705
BstC8I	GCNNGC	8 cut(s)	123, 199, 573, 1319, 1476, 2190, 2632, 2991
BstDEI	CTNAG	8 cut(s)	152, 420, 1123, 1560, 2184, 2228, 2433, 2645
BstDSI	CCRYGG	2 cut(s)	159, 232
BstEII	GGTNACC	2 cut(s)	1393, 1618
BstF5I	GGATG	8 cut(s)	240, 544, 1663, 1789, 2092, 2963, 2977, 3186
BstFNI	CGCG	2 cut(s)	234, 306
BstH2I	RGCGCY	3 cut(s)	122, 232, 2145
BstHHI	GCGC	5 cut(s)	121, 231, 2144, 2282, 3061
BstMAI	GTCTC	6 cut(s)	484, 812, 839, 876, 998, 2751
BstMCI	CGRYCG	1 cut(s)	102
BstNI	CCWGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
BstNSI	RCATGY	5 cut(s)	571, 869, 1585, 2796, 2942
BstPAI	GACNNNNGTC	1 cut(s)	868
BstPI	GGTNACC	2 cut(s)	1393, 1618
BstSCI	CCNGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
BstSFI	CTRYAG	4 cut(s)	276, 331, 2283, 3313
BstSLI	GKGCMC	1 cut(s)	1581
BstUI	CGCG	2 cut(s)	234, 306
BstV1I	GCAGC	6 cut(s)	412, 562, 1850, 1853, 2155, 2269
BstV2I	GAAGAC	1 cut(s)	827
BstX2I	RGATCY	6 cut(s)	337, 1297, 1816, 2680, 2726, 2971
BstXI	CCANNNNNNTGG	1 cut(s)	3197
BstYI	RGATCY	6 cut(s)	337, 1297, 1816, 2680, 2726, 2971
Bsu36I	CCTNAGG	1 cut(s)	420
BsuI	GTATCC	1 cut(s)	2495
BsuRI	GGCC	4 cut(s)	1194, 1565, 2541, 3244
BtgI	CCRYGG	2 cut(s)	159, 232
BtsCI	GGATG	8 cut(s)	240, 544, 1663, 1789, 2092, 2963, 2977, 3186
BtsI	GCAGTG	4 cut(s)	642, 1146, 2615, 2991
BtsIMutI	CAGTG	6 cut(s)	642, 1146, 1314, 2615, 2991, 3092
BtuMI	TCGCGA	1 cut(s)	306
Cac8I	GCNNGC	8 cut(s)	123, 199, 573, 1319, 1476, 2190, 2632, 2991
CaiI	CAGNNNCTG	1 cut(s)	391
CciI	TCATGA	1 cut(s)	346
CfoI	GCGC	5 cut(s)	121, 231, 2144, 2282, 3061
Cfr10I	RCCGGY	2 cut(s)	191, 209
Cfr13I	GGNCC	7 cut(s)	156, 272, 923, 2404, 2540, 2659, 3183
Cfr42I	CCGCGG	1 cut(s)	235
CseI	GACGC	2 cut(s)	44, 858
Csp6I	GTAC	9 cut(s)	495, 585, 780, 1452, 1557, 1931, 2367, 2931, 3179
CspCI	CAANNNNNGTGG	4 cut(s)	196, 231, 1776, 1811
CviQI	GTAC	9 cut(s)	495, 585, 780, 1452, 1557, 1931, 2367, 2931, 3179
DdeI	CTNAG	8 cut(s)	152, 420, 1123, 1560, 2184, 2228, 2433, 2645
DraIII	CACNNNGTG	1 cut(s)	165
DriI	GACNNNNNGTC	1 cut(s)	1994
Eam1104I	CTCTTC	4 cut(s)	396, 1573, 2553, 3087
Eam1105I	GACNNNNNGTC	1 cut(s)	1994
EarI	CTCTTC	4 cut(s)	396, 1573, 2553, 3087
EciI	GGCGGA	1 cut(s)	127
Eco147I	AGGCCT	1 cut(s)	1565
Eco31I	GGTCTC	1 cut(s)	484
Eco47I	GGWCC	6 cut(s)	156, 272, 923, 2404, 2659, 3183
Eco57I	CTGAAG	6 cut(s)	74, 204, 413, 770, 1674, 3068
Eco81I	CCTNAGG	1 cut(s)	420
Eco88I	CYCGRG	3 cut(s)	1326, 1335, 1668
Eco91I	GGTNACC	2 cut(s)	1393, 1618
EcoO109I	RGGNCCY	1 cut(s)	2540
EcoO65I	GGTNACC	2 cut(s)	1393, 1618
EcoRI	GAATTC	1 cut(s)	1801
EcoRII	CCWGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
EcoT22I	ATGCAT	1 cut(s)	1587
Esp3I	CGTCTC	2 cut(s)	839, 876
FalI	AAGNNNNNCTT	2 cut(s)	3215, 3247
FaqI	GGGAC	3 cut(s)	119, 1966, 2001
FauI	CCCGC	5 cut(s)	81, 145, 227, 2639, 2989
FauNDI	CATATG	2 cut(s)	2122, 2525
FblI	GTMKAC	1 cut(s)	1498
Fnu4HI	GCNGC	9 cut(s)	189, 232, 426, 576, 1839, 1842, 2052, 2169, 2283
FokI	GGATG	8 cut(s)	227, 551, 1650, 1796, 2079, 2964, 2970, 3173
Fsp4HI	GCNGC	9 cut(s)	189, 232, 426, 576, 1839, 1842, 2052, 2169, 2283
FspBI	CTAG	8 cut(s)	198, 600, 626, 1170, 1856, 2253, 2582, 3308
GlaI	GCGC	5 cut(s)	120, 230, 2143, 2281, 3060
GluI	GCNGC	9 cut(s)	189, 232, 426, 576, 1839, 1842, 2052, 2169, 2283
GsaI	CCCAGC	1 cut(s)	3059
GsuI	CTGGAG	2 cut(s)	1257, 2397
HaeII	RGCGCY	3 cut(s)	122, 232, 2145
HaeIII	GGCC	4 cut(s)	1194, 1565, 2541, 3244
HapII	CCGG	6 cut(s)	39, 192, 210, 341, 611, 3008
HgaI	GACGC	2 cut(s)	44, 858
HhaI	GCGC	5 cut(s)	121, 231, 2144, 2282, 3061
Hin1I	GRCGYC	1 cut(s)	36
Hin6I	GCGC	5 cut(s)	119, 229, 2142, 2280, 3059
HinP1I	GCGC	5 cut(s)	119, 229, 2142, 2280, 3059
HincII	GTYRAC	1 cut(s)	2002
HindII	GTYRAC	1 cut(s)	2002
HindIII	AAGCTT	1 cut(s)	2855
HinfI	GANTC	9 cut(s)	26, 406, 737, 905, 1027, 1676, 2207, 3115, 3272
HpaII	CCGG	6 cut(s)	39, 192, 210, 341, 611, 3008
HphI	GGTGA	9 cut(s)	238, 482, 625, 893, 962, 1387, 1612, 1784, 1838
Hpy166II	GTNNAC	8 cut(s)	802, 1078, 1499, 1931, 2002, 2101, 2312, 3179
Hpy8I	GTNNAC	8 cut(s)	802, 1078, 1499, 1931, 2002, 2101, 2312, 3179
Hpy99I	CGWCG	7 cut(s)	134, 146, 149, 224, 1748, 2202, 2906
HpyCH4III	ACNGT	8 cut(s)	387, 1075, 1399, 1468, 1748, 1795, 2014, 3075
HpyCH4IV	ACGT	6 cut(s)	129, 1864, 2197, 2314, 2704, 3218
HpyF3I	CTNAG	8 cut(s)	152, 420, 1123, 1560, 2184, 2228, 2433, 2645
HpySE526I	ACGT	6 cut(s)	129, 1864, 2197, 2314, 2704, 3218
Hsp92I	GRCGYC	1 cut(s)	36
HspAI	GCGC	5 cut(s)	119, 229, 2142, 2280, 3059
Kpn2I	TCCGGA	1 cut(s)	3007
KpnI	GGTACC	1 cut(s)	588
KspI	CCGCGG	1 cut(s)	235
LguI	GCTCTTC	1 cut(s)	3087
LmnI	GCTCC	4 cut(s)	526, 850, 943, 2462
Lsp1109I	GCAGC	6 cut(s)	412, 562, 1850, 1853, 2155, 2269
MaeI	CTAG	8 cut(s)	198, 600, 626, 1170, 1856, 2253, 2582, 3308
MaeII	ACGT	6 cut(s)	129, 1864, 2197, 2314, 2704, 3218
MaeIII	GTNAC	7 cut(s)	458, 790, 1393, 1618, 2266, 2315, 2788
MfeI	CAATTG	1 cut(s)	2061
MflI	RGATCY	6 cut(s)	337, 1297, 1816, 2680, 2726, 2971
MhlI	GDGCHC	4 cut(s)	1336, 1581, 2536, 2995
MmeI	TCCRAC	1 cut(s)	415
Mph1103I	ATGCAT	1 cut(s)	1587
MroI	TCCGGA	1 cut(s)	3007
MroXI	GAANNNNTTC	2 cut(s)	312, 405
MseI	TTAA	6 cut(s)	1260, 2859, 3102, 3194, 3215, 3300
MslI	CAYNNNNRTG	5 cut(s)	1625, 1912, 2106, 3066, 3195
MspA1I	CMGCKG	1 cut(s)	234
MspI	CCGG	6 cut(s)	39, 192, 210, 341, 611, 3008
MspR9I	CCNGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
MunI	CAATTG	1 cut(s)	2061
Mva1269I	GAATGC	2 cut(s)	652, 897
MvaI	CCWGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
MvnI	CGCG	2 cut(s)	234, 306
NdeI	CATATG	2 cut(s)	2122, 2525
NheI	GCTAGC	1 cut(s)	197
NlaIV	GGNNCC	9 cut(s)	339, 586, 915, 945, 1750, 2405, 2660, 2784, 2973
NmeAIII	GCCGAG	1 cut(s)	1727
NmuCI	GTSAC	3 cut(s)	1393, 1618, 2266
NruI	TCGCGA	1 cut(s)	306
NsiI	ATGCAT	1 cut(s)	1587
NspI	RCATGY	5 cut(s)	571, 869, 1585, 2796, 2942
PaeR7I	CTCGAG	1 cut(s)	1335
PagI	TCATGA	1 cut(s)	346
PceI	AGGCCT	1 cut(s)	1565
PciI	ACATGT	1 cut(s)	2938
PciSI	GCTCTTC	1 cut(s)	3087
PcsI	WCGNNNNNNNCGW	2 cut(s)	138, 1333
PctI	GAATGC	2 cut(s)	652, 897
PdmI	GAANNNNTTC	2 cut(s)	312, 405
PfeI	GAWTC	9 cut(s)	26, 406, 737, 905, 1027, 1676, 2207, 3115, 3272
PflFI	GACNNNGTC	2 cut(s)	831, 2762
PkrI	GCNGC	9 cut(s)	190, 233, 427, 577, 1840, 1843, 2053, 2170, 2284
Ppu21I	YACGTR	1 cut(s)	2705
PscI	ACATGT	1 cut(s)	2938
PshAI	GACNNNNGTC	1 cut(s)	868
Psp1406I	AACGTT	1 cut(s)	3218
Psp6I	CCWGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
PspEI	GGTNACC	2 cut(s)	1393, 1618
PspFI	CCCAGC	1 cut(s)	3055
PspGI	CCWGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
PspN4I	GGNNCC	9 cut(s)	339, 586, 915, 945, 1750, 2405, 2660, 2784, 2973
PspPI	GGNCC	7 cut(s)	156, 272, 923, 2404, 2540, 2659, 3183
PspXI	VCTCGAGB	1 cut(s)	1335
PstI	CTGCAG	1 cut(s)	2287
PstNI	CAGNNNCTG	1 cut(s)	391
PsuI	RGATCY	6 cut(s)	337, 1297, 1816, 2680, 2726, 2971
PsyI	GACNNNGTC	2 cut(s)	831, 2762
RruI	TCGCGA	1 cut(s)	306
RsaI	GTAC	9 cut(s)	496, 586, 781, 1453, 1558, 1932, 2368, 2932, 3180
RsaNI	GTAC	9 cut(s)	495, 585, 780, 1452, 1557, 1931, 2367, 2931, 3179
RseI	CAYNNNNRTG	5 cut(s)	1625, 1912, 2106, 3066, 3195
SacII	CCGCGG	1 cut(s)	235
SapI	GCTCTTC	1 cut(s)	3087
SaqAI	TTAA	6 cut(s)	1260, 2859, 3102, 3194, 3215, 3300
SatI	GCNGC	9 cut(s)	189, 232, 426, 576, 1839, 1842, 2052, 2169, 2283
Sau96I	GGNCC	7 cut(s)	156, 272, 923, 2404, 2540, 2659, 3183
ScaI	AGTACT	1 cut(s)	1453
ScrFI	CCNGG	7 cut(s)	270, 375, 918, 948, 1215, 1391, 2073
SduI	GDGCHC	4 cut(s)	1336, 1581, 2536, 2995
SfcI	CTRYAG	4 cut(s)	276, 331, 2283, 3313
Sfr274I	CTCGAG	1 cut(s)	1335
Sfr303I	CCGCGG	1 cut(s)	235
SgrAI	CRCCGGYG	1 cut(s)	191
SgrBI	CCGCGG	1 cut(s)	235
SinI	GGWCC	6 cut(s)	156, 272, 923, 2404, 2659, 3183
SlaI	CTCGAG	1 cut(s)	1335
SmiMI	CAYNNNNRTG	5 cut(s)	1625, 1912, 2106, 3066, 3195
SmlI	CTYRAG	4 cut(s)	1292, 1335, 1892, 2535
SmoI	CTYRAG	4 cut(s)	1292, 1335, 1892, 2535
SseBI	AGGCCT	1 cut(s)	1565
SspI	AATATT	1 cut(s)	2293
SspMI	CTAG	8 cut(s)	198, 600, 626, 1170, 1856, 2253, 2582, 3308
StuI	AGGCCT	1 cut(s)	1565
StyD4I	CCNGG	7 cut(s)	268, 373, 916, 946, 1213, 1389, 2071
TaaI	ACNGT	8 cut(s)	387, 1075, 1399, 1468, 1748, 1795, 2014, 3075
TaiI	ACGT	6 cut(s)	132, 1867, 2200, 2317, 2707, 3221
TaqII	GACCGA	1 cut(s)	116
TatI	WGTACW	4 cut(s)	1451, 1556, 1930, 2366
TauI	GCSGC	3 cut(s)	191, 234, 2054
TfiI	GAWTC	9 cut(s)	26, 406, 737, 905, 1027, 1676, 2207, 3115, 3272
Tru1I	TTAA	6 cut(s)	1260, 2859, 3102, 3194, 3215, 3300
Tru9I	TTAA	6 cut(s)	1260, 2859, 3102, 3194, 3215, 3300
TscAI	CASTG	6 cut(s)	642, 1146, 1314, 2622, 2991, 3092
TseFI	GTSAC	3 cut(s)	1393, 1618, 2266
TseI	GCWGC	6 cut(s)	425, 575, 1838, 1841, 2168, 2282
Tsp45I	GTSAC	3 cut(s)	1393, 1618, 2266
TspGWI	ACGGA	4 cut(s)	863, 2789, 2864, 3196
TspRI	CASTG	6 cut(s)	642, 1146, 1314, 2622, 2991, 3092
Tth111I	GACNNNGTC	2 cut(s)	831, 2762
VpaK11BI	GGWCC	6 cut(s)	156, 272, 923, 2404, 2659, 3183
XapI	RAATTY	5 cut(s)	1174, 1801, 2067, 2441, 2589
XbaI	TCTAGA	1 cut(s)	2252
XceI	RCATGY	5 cut(s)	571, 869, 1585, 2796, 2942
XhoI	CTCGAG	1 cut(s)	1335
XmiI	GTMKAC	1 cut(s)	1498
XmnI	GAANNNNTTC	2 cut(s)	312, 405
XspI	CTAG	8 cut(s)	198, 600, 626, 1170, 1856, 2253, 2582, 3308
ZrmI	AGTACT	1 cut(s)	1453
Zsp2I	ATGCAT	1 cut(s)	1587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.