Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0004820

Physical Location & Seq

Genomic Coordinates Forward (+)

38340883 .. 38361385

Gene Structure 20503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG332500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGGCGATCCGAAGGACGACGAACTCTGCATGGAGATCGACCCGCCTTTCAGAGAAAGCACCGCCACGACCGAGGACTGGCGGAAGGCGCTTGCCAACGTCGTCCCCGCCGTCGTCGTTTTGAGGACCACGGCGTGTCGTGCCTTCGATACTGAAGCCGCCGGTGCTAGCTATGCCACCGGCTTTGTCGTCGATAAGCGACGCGGGATCATCCTCACCAACCGCCATGTTGTCAAGCCTGGACCTGTAGTTGCGGAAGCTATGTTTGTGAATCGCGAAGAAGTTCCAGTATATCCTATCTATAGGGATCCGGTTCATGATTTTGGCTTCTTTTGTTATGATCCTGGTGCAATACAGTTTCTGAAGTATGAAGAGATTCCTCTTGCCCCTGAGGCTGCTTCTGTTGGATTAGAAATCAGGGTTGTTGGTAACGATAGTGGTGAGAAGGTCTCCATTTTGGCTGGTACTCTTGCCCGTTTAGATAGAGATGCTCCTTATTACAAAAAGGATGGCTATAATGATTTCAATACATTCTACATGCAAGCTGCATCTGGTACCAAAGGTGGGTCTAGTGGCTCTCCGGTGATTGATTGGCTAGGTAGGGCAGTGGCTTTGAATGCTGGTAGCAAGTCATCAAGTGCATCAGCATTTTTTTTACCATTGGAACGAGTTGTGAGGGCATTGAAGTTTCTCCAAAATGGCAGAGATTCTTTTGTCAAAAAATGGGAGGCAGTTTCTATACTGCGTGGTACGCTTCAGGTAACTTTTGTCCACAAAGGATTTGATGAGACACGGCGACTTGGTCTTCGAAGTGAGACGGAGCAGAGGGTTCGACATGCGTCTCCGCAGGGTGAAACTGGAATGCTTGTTGTTGAATCTGTGGTGCCAGGCGGTCCAGCTCATAAGTGTTTGGAGCCAGGTGATGTTCTTGTTTGCATGAATGGGGAAGTAATTACCCAATTTCTAAAAATGGAGACATTACTTGATGATAGTGTGAATCAAAAAATTGAAATACAGATCGAAAGGGGTGGCACACCATTTACTGTGAACTTGCTGGTGCAGGATTTGCACTCAATAACCCCTAATTACTTCTTAGAAGTAAGTGGTGCAGTGATACACCCTCTCTCGTATCAACAGGCTAGAAATTTTCGCTTCCAGTGTGGCCTTGTGTATGTGGCAGAGCCAGGATATATGCTCTTTAGAGCTGGAGTTCCTCGTCATGCTATCATTAAGAAGTTTGCTGGTCAGGAAATATCACTACTTGAGGATCTAATTTCAGTGCTATGCAAGCTATCTCGGGGTGCTCGAGTGCCATTAGAGTATATAAGCTATGCAGATCGTCATCGAAGGAAGTCAGTCCTGGTCACCGTTGATCGCCATGAATGGTATGCCCCTCCACAGATATACACTCGTGATGATAGTACTGGTCTTTGGACTGTGAAGCCTGCTTTTCAGCCTGATGCTGGTCTACTTTCATCTGGTATCAATGATCTTGGTGGTATAAGAAGTGATGCAGTTCCTCTATGTACTGAGGCCTCTTCTGTTGGGCACATGCATCGTAATAGCCATGCAGAATTGAATGAAGGGGTCACCAGTATGGAAACCAGTTATGAACATTCTTCTGAAGGAGGACATCCTCGGGCTGAATCTGATGTTGAAACAAAGAAACGCCGAGTGAAGGAGGATTTTTCTTCTGATGGAAATGTCGTAGCCGACGGTTCCTTTCTTGAAACAGATGAAGGTGATCCGGAGGATGCAAATACTGTGGAGAATTCTGTTATGAGAGATCTTCAAGGTGAAAATGTAGCAGCAGCTAATTCTTCACTAGCTGAACGTATTATCGAGCCAACTCTTGTTATGCTTGAGGTTCATGTGCCACCATCATGTATGCTCGATGGTGTACATTCACAACATTTTTTTGGGACTGGTGTTATTATATACCACTCTCAAAATATGGGACTGATTGCTGTTGACAAGAATACAGTTGTGGTATCTGCATCTGATGTGATGCTCTCATTCGCCGCATTTCCAATTGAAATTCCAGGGGAGGTGGTTTTTCTCCATCCCGTTCACAATTATGCTCTCATTTCATATGATCCATTGGCCCTGGGCGCTGTTGGTATCTCGCTTGTTCGGGCTGCGAAACTACTTCCTGAGCCTGCAATACGTCGTGGGGATTCTGTGCATCTTGTTGGGCTAAGTAGAAGCCTACAAGCAACATCTAGAAAGTCTATTGTGACCAACCCATGCGCTGCAGTAAATATTAGCTCTGCTGATTGTCCACGTTACAGAGCAACCAATATGGAAGTGATCGAGCTTGATACTGATTTTGGCAGTACATTCTCTGGAGTGCTAACTGATGAGCATGGAAGGGTCCGAGCCATTTGGGGTAGCTTTTCAACTCAGTTGAAATTTGGTTGCAGTAGCTCCGAAGATCATCAATTTGTAAGAGGTATCCCAATATATACAATCAGTCAAGTCCTTGAACAAATCATATGTGGTGCTCAAGGCCCTCCTCTTCTCATAAATTGTGTCAAAAGGACGATGCCTCTAGTTAGAATTTTGGAGGTTGAACTATATCCTACACTGCTTTCAAAAGCCCGCAGTTTTGGACTGAGTGATGATTGGGTCCAAGCACTTGTCAAGAAAGATCCGATTAGACGACAGGTTTTACGTGTGAAAGGATGTTTAGCTGGATCTAAAGCCGAAAATCTGTTAGAACAAGGAGACATGGTCTTGGCAATCAATAAGGAACCAGTTACATGCTTCCGTGATGTAGAAAATGCCTGTCAAGAATTGGGAAAACATGAAAACAAAGATGGAAAGCTTAACATGACCATATTCCGTCAGGGGCGTGAGATTGATCTTCTCGTCGAAACAGATATTAGAGATGGGAGTGGTACTACACGTGTAATAAATTGGTGTGGATGTATTGTTCAGGATCCCCATCCCGCAGTGCGTGCTCTTGGATTTCTTCCAGACGAAGGTCATGGTGTTTATGTGGCAAGGTCTTTGGGCACTTGCAGCAACGAAAGAAAAAAGTGTTGTGCAGCTGTGAGTTGGTGGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009642 GO:0009644 GO:0009892 GO:0010109 GO:0010205 GO:0016787 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0042548 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:1905156

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_1 PDZ_2 Trypsin_2

Protein Analysis

1023

Amino Acids

112.32

Weight (kDa)

5.76

Isoelectric Point (pI)

40.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	59 - 205	7.8e-21	Trypsin-like peptidase domain
PDZ_6	PF17820	290 - 342	1.9e-07	PDZ domain
PDZ_1	PF12812	358 - 431	1.3e-14	PDZ-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004820)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03380 AT3G03380
fragaria_vesca	FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890 FvH4_5g27890
malus_domestica	MD17G1256700.v1.1
prunus_persica	Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1 Prupe.3G159400_v2.0.a1
pyrus_communis	pycom17g25890
rosa_chinensis	RchiOBHm_Chr7g0220101
rosa_laevigata	RLG00000002258
rosa_multiflora	Rmu_sc0007553.1_g000006 Rmu_sc0018981.1_g000001
rosa_roxburghii	Rroxscaffold_3G00239790
rosa_rugosa	Rorug07G0188800 Rorug07G0188900 Rorug07G0188900 Rorug07G0189000
rosa_samantha	Rh7AG332500 Rh7BG322400 Rh7CG350000 Rh7DG328200
rosa_wichuraiana	Rw7G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	554
AccB1I	GGYRCC	2 cut(s)	554, 883
AccI	GTMKAC	1 cut(s)	1468
AccII	CGCG	2 cut(s)	204, 276
AccIII	TCCGGA	1 cut(s)	1747
AcsI	RAATTY	5 cut(s)	1144, 1771, 2037, 2411, 2559
AcuI	CTGAAG	4 cut(s)	174, 383, 740, 1644
AcvI	CACGTG	1 cut(s)	2909
AdeI	CACNNNGTG	1 cut(s)	135
AfaI	GTAC	8 cut(s)	466, 556, 751, 1423, 1528, 1902, 2338, 2902
AfiI	CCNNNNNNNGG	5 cut(s)	78, 564, 1463, 1678, 2984
AflIII	ACRYGT	3 cut(s)	2674, 2906, 2908
AjnI	CCWGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
AloI	GAACNNNNNNTCC	2 cut(s)	2919, 2951
Alw21I	GWGCWC	3 cut(s)	1306, 2506, 2965
Alw26I	GTCTC	6 cut(s)	454, 782, 809, 846, 968, 2721
AlwNI	CAGNNNCTG	1 cut(s)	361
Ama87I	CYCGRG	3 cut(s)	1296, 1305, 1638
Aor13HI	TCCGGA	1 cut(s)	1747
AoxI	GGCC	4 cut(s)	1162, 1533, 2103, 2509
ApeKI	GCWGC	8 cut(s)	395, 545, 1808, 1811, 2138, 2252, 3024, 3050
ApoI	RAATTY	5 cut(s)	1144, 1771, 2037, 2411, 2559
ArsI	GACNNNNNNTTYG	2 cut(s)	789, 821
Asp700I	GAANNNNTTC	2 cut(s)	282, 375
Asp718I	GGTACC	1 cut(s)	554
AspLEI	GCGC	3 cut(s)	91, 2114, 2252
AspS9I	GGNCC	7 cut(s)	126, 242, 893, 2104, 2374, 2510, 2629
AsuHPI	GGTGA	9 cut(s)	208, 452, 595, 863, 932, 1357, 1582, 1754, 1808
AsuII	TTCGAA	1 cut(s)	808
AsuNHI	GCTAGC	1 cut(s)	167
AvaI	CYCGRG	3 cut(s)	1296, 1305, 1638
AvaII	GGWCC	5 cut(s)	126, 242, 893, 2374, 2629
AxyI	CCTNAGG	1 cut(s)	390
BaeGI	GKGCMC	2 cut(s)	1551, 3020
BamHI	GGATCC	2 cut(s)	307, 2941
BanI	GGYRCC	2 cut(s)	554, 883
BauI	CACGAG	1 cut(s)	1410
BbrPI	CACGTG	1 cut(s)	2909
BbsI	GAAGAC	1 cut(s)	797
Bbv12I	GWGCWC	3 cut(s)	1306, 2506, 2965
BbvI	GCAGC	8 cut(s)	382, 532, 1820, 1823, 2125, 2239, 3036, 3062
BccI	CCATC	8 cut(s)	503, 1691, 1888, 1889, 2070, 2813, 2885, 2955
BceAI	ACGGC	3 cut(s)	95, 147, 809
BcgI	CGANNNNNNTGC	6 cut(s)	19, 53, 812, 846, 1538, 1572
BciT130I	CCWGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
BciVI	GTATCC	1 cut(s)	2465
BcoDI	GTCTC	6 cut(s)	454, 782, 809, 846, 968, 2721
BfaI	CTAG	7 cut(s)	168, 570, 596, 1140, 1826, 2223, 2552
BfmI	CTRYAG	3 cut(s)	246, 301, 2253
BfoI	RGCGCY	2 cut(s)	92, 2115
BfuI	GTATCC	1 cut(s)	2465
BglI	GCCNNNNNGGC	1 cut(s)	392
BglII	AGATCT	1 cut(s)	1786
BmcAI	AGTACT	1 cut(s)	1423
Bme1390I	CCNGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
Bme18I	GGWCC	5 cut(s)	126, 242, 893, 2374, 2629
BmeT110I	CYCGRG	3 cut(s)	1296, 1305, 1638
BmgT120I	GGNCC	7 cut(s)	126, 242, 893, 2104, 2374, 2510, 2629
BmiI	GGNNCC	9 cut(s)	309, 556, 885, 915, 1720, 2375, 2630, 2754, 2943
BmrFI	CCNGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
BmtI	GCTAGC	1 cut(s)	171
BoxI	GACNNNNGTC	2 cut(s)	838, 2985
BpiI	GAAGAC	1 cut(s)	797
BplI	GAGNNNNNCTC	2 cut(s)	434, 466
BpmI	CTGGAG	2 cut(s)	1227, 2367
Bpu10I	CCTNAGC	1 cut(s)	2154
Bpu14I	TTCGAA	1 cut(s)	808
BpuEI	CTTGAG	3 cut(s)	1283, 1883, 2490
BsaAI	YACGTR	2 cut(s)	2675, 2909
BsaBI	GATNNNNATC	2 cut(s)	1341, 2946
BsaI	GGTCTC	1 cut(s)	454
BsaJI	CCNNGG	6 cut(s)	72, 129, 1637, 2042, 2106, 2107
BsaWI	WCCGGW	3 cut(s)	310, 580, 1747
BsaXI	ACNNNNNCTCC	2 cut(s)	2718, 2748
Bsc4I	CCNNNNNNNGG	5 cut(s)	78, 564, 1463, 1678, 2984
Bse118I	RCCGGY	2 cut(s)	161, 179
Bse1I	ACTGG	9 cut(s)	83, 287, 862, 1156, 1429, 1593, 1605, 1930, 2756
Bse21I	CCTNAGG	1 cut(s)	390
Bse8I	GATNNNNATC	2 cut(s)	1341, 2946
BseAI	TCCGGA	1 cut(s)	1747
BseBI	CCWGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
BseDI	CCNNGG	6 cut(s)	72, 129, 1637, 2042, 2106, 2107
BseGI	GGATG	8 cut(s)	210, 514, 1633, 1759, 2062, 2690, 2933, 2947
BseJI	GATNNNNATC	2 cut(s)	1341, 2946
BseLI	CCNNNNNNNGG	5 cut(s)	78, 564, 1463, 1678, 2984
BseMII	CTCAG	5 cut(s)	381, 1521, 2145, 2417, 2606
BseNI	ACTGG	9 cut(s)	83, 287, 862, 1156, 1429, 1593, 1605, 1930, 2756
BseRI	GAGGAG	1 cut(s)	2505
BseSI	GKGCMC	2 cut(s)	1551, 3020
BseXI	GCAGC	8 cut(s)	382, 532, 1820, 1823, 2125, 2239, 3036, 3062
BsgI	GTGCAG	3 cut(s)	1079, 1128, 3069
Bsh1236I	CGCG	2 cut(s)	204, 276
Bsh1285I	CGRYCG	1 cut(s)	72
BshFI	GGCC	4 cut(s)	1164, 1535, 2105, 2511
BshNI	GGYRCC	2 cut(s)	554, 883
BsiEI	CGRYCG	1 cut(s)	72
BsiHKAI	GWGCWC	3 cut(s)	1306, 2506, 2965
BsiHKCI	CYCGRG	3 cut(s)	1296, 1305, 1638
BsiSI	CCGG	5 cut(s)	162, 180, 311, 581, 1748
BslFI	GGGAC	3 cut(s)	89, 1936, 1971
BslI	CCNNNNNNNGG	5 cut(s)	78, 564, 1463, 1678, 2984
BsmAI	GTCTC	6 cut(s)	454, 782, 809, 846, 968, 2721
BsmBI	CGTCTC	2 cut(s)	809, 846
BsmFI	GGGAC	3 cut(s)	89, 1936, 1971
BsmI	GAATGC	2 cut(s)	622, 867
BsnI	GGCC	4 cut(s)	1164, 1535, 2105, 2511
Bso31I	GGTCTC	1 cut(s)	454
BsoBI	CYCGRG	3 cut(s)	1296, 1305, 1638
Bsp119I	TTCGAA	1 cut(s)	808
Bsp1286I	GDGCHC	5 cut(s)	1306, 1551, 2506, 2965, 3020
Bsp13I	TCCGGA	1 cut(s)	1747
Bsp1407I	TGTACA	1 cut(s)	1900
Bsp68I	TCGCGA	1 cut(s)	276
BspANI	GGCC	4 cut(s)	1164, 1535, 2105, 2511
BspCNI	CTCAG	5 cut(s)	382, 1522, 2146, 2416, 2607
BspEI	TCCGGA	1 cut(s)	1747
BspFNI	CGCG	2 cut(s)	204, 276
BspHI	TCATGA	1 cut(s)	316
BspLI	GGNNCC	9 cut(s)	309, 556, 885, 915, 1720, 2375, 2630, 2754, 2943
BspMAI	CTGCAG	1 cut(s)	2257
BspOI	GCTAGC	1 cut(s)	171
BspT104I	TTCGAA	1 cut(s)	808
BspT107I	GGYRCC	2 cut(s)	554, 883
BspTNI	GGTCTC	1 cut(s)	454
BsrFI	RCCGGY	2 cut(s)	161, 179
BsrGI	TGTACA	1 cut(s)	1900
BsrI	ACTGG	9 cut(s)	83, 287, 862, 1156, 1429, 1593, 1605, 1930, 2756
BssAI	RCCGGY	2 cut(s)	161, 179
BssECI	CCNNGG	6 cut(s)	72, 129, 1637, 2042, 2106, 2107
BssSI	CACGAG	1 cut(s)	1410
Bst2BI	CACGAG	1 cut(s)	1410
Bst2UI	CCWGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
Bst4CI	ACNGT	7 cut(s)	357, 1045, 1369, 1438, 1718, 1765, 1984
Bst6I	CTCTTC	3 cut(s)	366, 1543, 2523
BstAPI	GCANNNNNTGC	2 cut(s)	1066, 2960
BstAUI	TGTACA	1 cut(s)	1900
BstBAI	YACGTR	2 cut(s)	2675, 2909
BstBI	TTCGAA	1 cut(s)	808
BstC8I	GCNNGC	8 cut(s)	93, 169, 543, 1289, 1446, 2160, 2602, 2961
BstDEI	CTNAG	7 cut(s)	390, 1093, 1530, 2154, 2198, 2403, 2615
BstDSI	CCRYGG	1 cut(s)	129
BstEII	GGTNACC	2 cut(s)	1363, 1588
BstF5I	GGATG	8 cut(s)	210, 514, 1633, 1759, 2062, 2690, 2933, 2947
BstFNI	CGCG	2 cut(s)	204, 276
BstH2I	RGCGCY	2 cut(s)	92, 2115
BstHHI	GCGC	3 cut(s)	91, 2114, 2252
BstMAI	GTCTC	6 cut(s)	454, 782, 809, 846, 968, 2721
BstMCI	CGRYCG	1 cut(s)	72
BstNI	CCWGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
BstNSI	RCATGY	4 cut(s)	541, 839, 1555, 2766
BstPAI	GACNNNNGTC	2 cut(s)	838, 2985
BstPI	GGTNACC	2 cut(s)	1363, 1588
BstSCI	CCNGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
BstSFI	CTRYAG	3 cut(s)	246, 301, 2253
BstSLI	GKGCMC	2 cut(s)	1551, 3020
BstUI	CGCG	2 cut(s)	204, 276
BstV1I	GCAGC	8 cut(s)	382, 532, 1820, 1823, 2125, 2239, 3036, 3062
BstV2I	GAAGAC	1 cut(s)	797
BstX2I	RGATCY	6 cut(s)	307, 1267, 1786, 2650, 2696, 2941
BstYI	RGATCY	6 cut(s)	307, 1267, 1786, 2650, 2696, 2941
Bsu36I	CCTNAGG	1 cut(s)	390
BsuI	GTATCC	1 cut(s)	2465
BsuRI	GGCC	4 cut(s)	1164, 1535, 2105, 2511
BtgI	CCRYGG	1 cut(s)	129
BtsCI	GGATG	8 cut(s)	210, 514, 1633, 1759, 2062, 2690, 2933, 2947
BtsI	GCAGTG	4 cut(s)	612, 1116, 2585, 2961
BtsIMutI	CAGTG	6 cut(s)	612, 1116, 1163, 1284, 2585, 2961
BtuMI	TCGCGA	1 cut(s)	276
Cac8I	GCNNGC	8 cut(s)	93, 169, 543, 1289, 1446, 2160, 2602, 2961
CaiI	CAGNNNCTG	1 cut(s)	361
CciI	TCATGA	1 cut(s)	316
CfoI	GCGC	3 cut(s)	91, 2114, 2252
Cfr10I	RCCGGY	2 cut(s)	161, 179
Cfr13I	GGNCC	7 cut(s)	126, 242, 893, 2104, 2374, 2510, 2629
CseI	GACGC	2 cut(s)	210, 828
Csp6I	GTAC	8 cut(s)	465, 555, 750, 1422, 1527, 1901, 2337, 2901
CspCI	CAANNNNNGTGG	4 cut(s)	166, 201, 1746, 1781
CviQI	GTAC	8 cut(s)	465, 555, 750, 1422, 1527, 1901, 2337, 2901
DdeI	CTNAG	7 cut(s)	390, 1093, 1530, 2154, 2198, 2403, 2615
DraIII	CACNNNGTG	1 cut(s)	135
Eam1104I	CTCTTC	3 cut(s)	366, 1543, 2523
EarI	CTCTTC	3 cut(s)	366, 1543, 2523
EciI	GGCGGA	1 cut(s)	97
Eco147I	AGGCCT	1 cut(s)	1535
Eco31I	GGTCTC	1 cut(s)	454
Eco47I	GGWCC	5 cut(s)	126, 242, 893, 2374, 2629
Eco57I	CTGAAG	4 cut(s)	174, 383, 740, 1644
Eco72I	CACGTG	1 cut(s)	2909
Eco81I	CCTNAGG	1 cut(s)	390
Eco88I	CYCGRG	3 cut(s)	1296, 1305, 1638
Eco91I	GGTNACC	2 cut(s)	1363, 1588
EcoO109I	RGGNCCY	1 cut(s)	2510
EcoO65I	GGTNACC	2 cut(s)	1363, 1588
EcoRI	GAATTC	1 cut(s)	1771
EcoRII	CCWGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
EcoT22I	ATGCAT	1 cut(s)	1557
Esp3I	CGTCTC	2 cut(s)	809, 846
FaqI	GGGAC	3 cut(s)	89, 1936, 1971
FauI	CCCGC	5 cut(s)	51, 115, 197, 2609, 2959
FauNDI	CATATG	2 cut(s)	2092, 2495
FblI	GTMKAC	1 cut(s)	1468
FokI	GGATG	8 cut(s)	197, 521, 1620, 1766, 2049, 2697, 2934, 2940
FspBI	CTAG	7 cut(s)	168, 570, 596, 1140, 1826, 2223, 2552
GlaI	GCGC	3 cut(s)	90, 2113, 2251
GsuI	CTGGAG	2 cut(s)	1227, 2367
HaeII	RGCGCY	2 cut(s)	92, 2115
HaeIII	GGCC	4 cut(s)	1164, 1535, 2105, 2511
HapII	CCGG	5 cut(s)	162, 180, 311, 581, 1748
HgaI	GACGC	2 cut(s)	210, 828
HhaI	GCGC	3 cut(s)	91, 2114, 2252
Hin6I	GCGC	3 cut(s)	89, 2112, 2250
HinP1I	GCGC	3 cut(s)	89, 2112, 2250
HincII	GTYRAC	1 cut(s)	1972
HindII	GTYRAC	1 cut(s)	1972
HindIII	AAGCTT	1 cut(s)	2825
HinfI	GANTC	7 cut(s)	271, 376, 707, 875, 997, 1646, 2177
HpaII	CCGG	5 cut(s)	162, 180, 311, 581, 1748
HphI	GGTGA	9 cut(s)	208, 452, 595, 863, 932, 1357, 1582, 1754, 1808
Hpy166II	GTNNAC	7 cut(s)	772, 1048, 1469, 1901, 1972, 2071, 2282
Hpy8I	GTNNAC	7 cut(s)	772, 1048, 1469, 1901, 1972, 2071, 2282
Hpy99I	CGWCG	9 cut(s)	23, 104, 116, 119, 194, 204, 1718, 2172, 2876
HpyCH4III	ACNGT	7 cut(s)	357, 1045, 1369, 1438, 1718, 1765, 1984
HpyCH4IV	ACGT	6 cut(s)	99, 1834, 2167, 2284, 2674, 2908
HpyF3I	CTNAG	7 cut(s)	390, 1093, 1530, 2154, 2198, 2403, 2615
HpySE526I	ACGT	6 cut(s)	99, 1834, 2167, 2284, 2674, 2908
HspAI	GCGC	3 cut(s)	89, 2112, 2250
Kpn2I	TCCGGA	1 cut(s)	1747
KpnI	GGTACC	1 cut(s)	558
LmnI	GCTCC	4 cut(s)	496, 820, 913, 2432
Lsp1109I	GCAGC	8 cut(s)	382, 532, 1820, 1823, 2125, 2239, 3036, 3062
MaeI	CTAG	7 cut(s)	168, 570, 596, 1140, 1826, 2223, 2552
MaeII	ACGT	6 cut(s)	99, 1834, 2167, 2284, 2674, 2908
MaeIII	GTNAC	7 cut(s)	428, 760, 1363, 1588, 2236, 2285, 2758
MfeI	CAATTG	1 cut(s)	2031
MflI	RGATCY	6 cut(s)	307, 1267, 1786, 2650, 2696, 2941
MhlI	GDGCHC	5 cut(s)	1306, 1551, 2506, 2965, 3020
MmeI	TCCRAC	1 cut(s)	385
Mph1103I	ATGCAT	1 cut(s)	1557
MroI	TCCGGA	1 cut(s)	1747
MroXI	GAANNNNTTC	2 cut(s)	282, 375
MseI	TTAA	2 cut(s)	1230, 2829
MslI	CAYNNNNRTG	3 cut(s)	1595, 1882, 2076
MspA1I	CMGCKG	1 cut(s)	3053
MspI	CCGG	5 cut(s)	162, 180, 311, 581, 1748
MspR9I	CCNGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
MunI	CAATTG	1 cut(s)	2031
Mva1269I	GAATGC	2 cut(s)	622, 867
MvaI	CCWGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
MvnI	CGCG	2 cut(s)	204, 276
NdeI	CATATG	2 cut(s)	2092, 2495
NheI	GCTAGC	1 cut(s)	167
NlaIV	GGNNCC	9 cut(s)	309, 556, 885, 915, 1720, 2375, 2630, 2754, 2943
NmeAIII	GCCGAG	1 cut(s)	1697
NmuCI	GTSAC	3 cut(s)	1363, 1588, 2236
NruI	TCGCGA	1 cut(s)	276
NsiI	ATGCAT	1 cut(s)	1557
NspI	RCATGY	4 cut(s)	541, 839, 1555, 2766
NspV	TTCGAA	1 cut(s)	808
PaeR7I	CTCGAG	1 cut(s)	1305
PagI	TCATGA	1 cut(s)	316
PasI	CCCWGGG	1 cut(s)	2107
PceI	AGGCCT	1 cut(s)	1535
PcsI	WCGNNNNNNNCGW	2 cut(s)	108, 1303
PctI	GAATGC	2 cut(s)	622, 867
PdmI	GAANNNNTTC	2 cut(s)	282, 375
PfeI	GAWTC	7 cut(s)	271, 376, 707, 875, 997, 1646, 2177
PflFI	GACNNNGTC	2 cut(s)	801, 2732
PmaCI	CACGTG	1 cut(s)	2909
PmlI	CACGTG	1 cut(s)	2909
Ppu21I	YACGTR	2 cut(s)	2675, 2909
PshAI	GACNNNNGTC	2 cut(s)	838, 2985
Psp6I	CCWGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
PspCI	CACGTG	1 cut(s)	2909
PspEI	GGTNACC	2 cut(s)	1363, 1588
PspGI	CCWGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
PspN4I	GGNNCC	9 cut(s)	309, 556, 885, 915, 1720, 2375, 2630, 2754, 2943
PspPI	GGNCC	7 cut(s)	126, 242, 893, 2104, 2374, 2510, 2629
PspXI	VCTCGAGB	1 cut(s)	1305
PstI	CTGCAG	1 cut(s)	2257
PstNI	CAGNNNCTG	1 cut(s)	361
PsuI	RGATCY	6 cut(s)	307, 1267, 1786, 2650, 2696, 2941
PsyI	GACNNNGTC	2 cut(s)	801, 2732
PvuII	CAGCTG	1 cut(s)	3053
RruI	TCGCGA	1 cut(s)	276
RsaI	GTAC	8 cut(s)	466, 556, 751, 1423, 1528, 1902, 2338, 2902
RsaNI	GTAC	8 cut(s)	465, 555, 750, 1422, 1527, 1901, 2337, 2901
RseI	CAYNNNNRTG	3 cut(s)	1595, 1882, 2076
SaqAI	TTAA	2 cut(s)	1230, 2829
Sau96I	GGNCC	7 cut(s)	126, 242, 893, 2104, 2374, 2510, 2629
ScaI	AGTACT	1 cut(s)	1423
ScrFI	CCNGG	8 cut(s)	240, 345, 888, 918, 1185, 1361, 2043, 2108
SduI	GDGCHC	5 cut(s)	1306, 1551, 2506, 2965, 3020
SfcI	CTRYAG	3 cut(s)	246, 301, 2253
Sfr274I	CTCGAG	1 cut(s)	1305
SfuI	TTCGAA	1 cut(s)	808
SgrAI	CRCCGGYG	1 cut(s)	161
SinI	GGWCC	5 cut(s)	126, 242, 893, 2374, 2629
SlaI	CTCGAG	1 cut(s)	1305
SmiMI	CAYNNNNRTG	3 cut(s)	1595, 1882, 2076
SmlI	CTYRAG	4 cut(s)	1262, 1305, 1862, 2505
SmoI	CTYRAG	4 cut(s)	1262, 1305, 1862, 2505
SseBI	AGGCCT	1 cut(s)	1535
SspI	AATATT	1 cut(s)	2263
SspMI	CTAG	7 cut(s)	168, 570, 596, 1140, 1826, 2223, 2552
StuI	AGGCCT	1 cut(s)	1535
StyD4I	CCNGG	8 cut(s)	238, 343, 886, 916, 1183, 1359, 2041, 2106
TaaI	ACNGT	7 cut(s)	357, 1045, 1369, 1438, 1718, 1765, 1984
TaiI	ACGT	6 cut(s)	102, 1837, 2170, 2287, 2677, 2911
TaqII	GACCGA	1 cut(s)	86
TatI	WGTACW	4 cut(s)	1421, 1526, 1900, 2336
TauI	GCSGC	2 cut(s)	161, 2024
TfiI	GAWTC	7 cut(s)	271, 376, 707, 875, 997, 1646, 2177
Tru1I	TTAA	2 cut(s)	1230, 2829
Tru9I	TTAA	2 cut(s)	1230, 2829
TscAI	CASTG	6 cut(s)	612, 1116, 1163, 1284, 2592, 2961
TseFI	GTSAC	3 cut(s)	1363, 1588, 2236
TseI	GCWGC	8 cut(s)	395, 545, 1808, 1811, 2138, 2252, 3024, 3050
Tsp45I	GTSAC	3 cut(s)	1363, 1588, 2236
TspGWI	ACGGA	3 cut(s)	833, 2759, 2834
TspRI	CASTG	6 cut(s)	612, 1116, 1163, 1284, 2592, 2961
Tth111I	GACNNNGTC	2 cut(s)	801, 2732
VpaK11BI	GGWCC	5 cut(s)	126, 242, 893, 2374, 2629
XapI	RAATTY	5 cut(s)	1144, 1771, 2037, 2411, 2559
XbaI	TCTAGA	1 cut(s)	2222
XceI	RCATGY	4 cut(s)	541, 839, 1555, 2766
XhoI	CTCGAG	1 cut(s)	1305
XmiI	GTMKAC	1 cut(s)	1468
XmnI	GAANNNNTTC	2 cut(s)	282, 375
XspI	CTAG	7 cut(s)	168, 570, 596, 1140, 1826, 2223, 2552
ZrmI	AGTACT	1 cut(s)	1423
Zsp2I	ATGCAT	1 cut(s)	1557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.