Prupe.1G259600_v2.0.a1

RNase H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001415
Physical Location & Seq
Forward (+)
26894723 .. 26898416
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G259600.1

Sequence Viewer

Length: 1113 bp
ATGAACTGCTTGTCGTACGTTTCGTCATACACAACCGCCATTTTCAAAAGGAATGGTCGTTTCATGGCGACGAGTTTTCTGTACAGACCTTGTTCGTGGAAAAGAAAATTGGACCTTTCGTGTGGGGTTAAGTGCATCAACTTAGAATCAACGAGATTCCATGTTCAGCTCTACTCGTCTCGAAGCAAAGCCAGCAGCAGCAAGACGCCGTCGTCTCGTACACGGAGGAAGTCAGAAGCTGAACCGGTTGTGGAGCTGGAAAAAGATGCCTTCTATGTAGTGCGCAAAGGAGATATTGTTGGTGTTTATAAGAGTTTCAGCGATTGTCAGGCTCAGCTTAGTTCTTCGATATTTGATCCTCCGGTTAGTGTGTACAAAGGATACTCTTTGCCTAAGGAAACTGAAGAGTATCTTGGTTCATGTGGACTTACGAATGCCATTTACACAATCGCGGCTGCAGATTTGAAGGACGATATTTTTGGCAAGCTTATGCATTGCCCTTTTCAAGATCCAGCATCTTTGCAAGGTGGAGCATCTGTCATGGATGCACTCAAAAAGAGATCCCATGAAGTGCCAACATCAGATAATTTGGAAGTAATTGGTTCACCTTCCATAGCAGATGATCCATTGAGGAAGCATGTCAAGATAGATCATTCCACTCAATCATTACCCTTAGATTCTGGCTTCTGTACTCTTGAGTTTGATGGTGCATCAAAAGGAAATCCTGGACTAGCTGGTGCTGGAGCTGTGCTGCGAGCTGATGATGGAAGTTTGATCTGTAAACTTCATGAAGGTCTGGGTGTTAGAACCAATAATGTTGCTGAGTACCGAGCTCTGATTCTAGGGCTAAAATATGCTCTTAAGAAAGGTTTTACTAAGATTCGTGTCAAAGGTGACTCCAAACTCGTCTGTATGCAGGTGCAGGGGTTATGGAAGGTCAGAAACCAGAACATGTCTGATTTGTGTGAAGAGGTGAAGGAACTGAAGGATAAATTTCTCTCCTTTGAGATCAGTCATGTTCTCAGGGAACTCAATTCTGAGGCAGATGCTCAAGCAAACTTGGCAGTCCGTCTTACGGATGGTCAAGTCCAGGAGGAGGAGTTTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cauli_VI DUF393 RVT_3

Protein Analysis

371

Amino Acids

40.83

Weight (kDa)

8.51

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
AarI CACCTGC 1 cut(s) 907
AasI GACNNNNNNGTC 1 cut(s) 211
Acc16I TGCGCA 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 907
AccII CGCG 1 cut(s) 452
AciI CCGC 2 cut(s) 36, 452
AclWI GGATC 4 cut(s) 350, 503, 555, 617
AcsI RAATTY 1 cut(s) 992
AcuI CTGAAG 2 cut(s) 423, 1004
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 6 cut(s) 17, 83, 220, 374, 691, 827
AfiI CCNNNNNNNGG 2 cut(s) 1075, 1096
AflII CTTAAG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 951
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 3 cut(s) 46, 466, 506
AjnI CCWGG 2 cut(s) 724, 1089
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 396, 428
AluBI AGCT 9 cut(s) 169, 239, 256, 337, 487, 734, 746, 758, 833
AluI AGCT 9 cut(s) 169, 239, 256, 337, 487, 734, 746, 758, 833
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 2 cut(s) 183, 219
AlwI GGATC 4 cut(s) 350, 503, 555, 617
AlwNI CAGNNNCTG 1 cut(s) 239
ApeKI GCWGC 4 cut(s) 195, 198, 455, 751
ApoI RAATTY 1 cut(s) 992
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AsiGI ACCGGT 1 cut(s) 244
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 597, 905, 985
AvaII GGWCC 1 cut(s) 112
AxyI CCTNAGG 1 cut(s) 393
BanII GRGCYC 1 cut(s) 835
Bbv12I GWGCWC 1 cut(s) 835
BbvI GCAGC 4 cut(s) 207, 210, 442, 738
BccI CCATC 3 cut(s) 698, 758, 1073
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 2 cut(s) 726, 1091
BciVI GTATCC 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 183, 219
BfaI CTAG 2 cut(s) 731, 842
BfmI CTRYAG 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 860
BfuAI ACCTGC 1 cut(s) 907
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 5 cut(s) 196, 199, 453, 456, 752
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 5 cut(s) 197, 200, 454, 457, 753
Bme1390I CCNGG 2 cut(s) 726, 1091
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmrFI CCNGG 2 cut(s) 726, 1091
BmsI GCATC 7 cut(s) 144, 256, 524, 535, 542, 719, 1036
BpmI CTGGAG 1 cut(s) 762
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 716, 1035
BsaHI GRCGYC 1 cut(s) 206
BsaWI WCCGGW 2 cut(s) 244, 361
BsaXI ACNNNNNCTCC 5 cut(s) 282, 312, 522, 552, 1085
Bsc4I CCNNNNNNNGG 2 cut(s) 1075, 1096
Bse118I RCCGGY 1 cut(s) 244
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 726, 1091
BseGI GGATG 2 cut(s) 550, 1084
BseLI CCNNNNNNNGG 2 cut(s) 1075, 1096
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 4 cut(s) 347, 813, 1029, 1036
BseRI GAGGAG 2 cut(s) 1109, 1112
BseXI GCAGC 4 cut(s) 207, 210, 442, 738
BsgI GTGCAG 1 cut(s) 941
Bsh1236I CGCG 1 cut(s) 452
BshTI ACCGGT 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 835
BsiSI CCGG 2 cut(s) 245, 362
BsiWI CGTACG 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 1075, 1096
BsmAI GTCTC 2 cut(s) 183, 219
BsmBI CGTCTC 2 cut(s) 183, 219
BsmI GAATGC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 835
Bsp1407I TGTACA 2 cut(s) 81, 372
Bsp143I GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 2 cut(s) 36, 452
BspCNI CTCAG 4 cut(s) 346, 814, 1030, 1035
BspFNI CGCG 1 cut(s) 452
BspHI TCATGA 1 cut(s) 787
BspMAI CTGCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 907
BspPI GGATC 4 cut(s) 350, 503, 555, 617
BspTI CTTAAG 1 cut(s) 860
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 244
BsrGI TGTACA 2 cut(s) 81, 372
BssAI RCCGGY 1 cut(s) 244
BssMI GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
BssNI GRCGYC 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 726, 1091
Bst6I CTCTTC 2 cut(s) 399, 963
BstACI GRCGYC 1 cut(s) 206
BstAFI CTTAAG 1 cut(s) 860
BstAUI TGTACA 2 cut(s) 81, 372
BstC8I GCNNGC 3 cut(s) 193, 485, 756
BstDEI CTNAG 9 cut(s) 142, 333, 338, 393, 673, 822, 876, 1022, 1038
BstF5I GGATG 2 cut(s) 550, 1084
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 7 cut(s) 358, 511, 563, 625, 652, 777, 1011
BstMAI GTCTC 2 cut(s) 183, 219
BstMBI GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
BstMWI GCNNNNNNNGC 2 cut(s) 192, 1061
BstNI CCWGG 2 cut(s) 726, 1091
BstNSI RCATGY 2 cut(s) 641, 955
BstSCI CCNGG 2 cut(s) 724, 1089
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 4 cut(s) 207, 210, 442, 738
BstX2I RGATCY 2 cut(s) 508, 560
BstYI RGATCY 2 cut(s) 508, 560
Bsu36I CCTNAGG 1 cut(s) 393
BsuI GTATCC 1 cut(s) 374
BtsCI GGATG 2 cut(s) 550, 1084
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 3 cut(s) 193, 485, 756
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 787
CfoI GCGC 1 cut(s) 285
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 112
CseI GACGC 1 cut(s) 214
Csp6I GTAC 6 cut(s) 16, 82, 219, 373, 690, 826
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 9 cut(s) 64, 161, 420, 541, 566, 638, 788, 952, 1016
CviQI GTAC 6 cut(s) 16, 82, 219, 373, 690, 826
DdeI CTNAG 9 cut(s) 142, 333, 338, 393, 673, 822, 876, 1022, 1038
DpnI GATC 7 cut(s) 357, 510, 562, 624, 651, 776, 1010
DpnII GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 399, 963
EarI CTCTTC 2 cut(s) 399, 963
Ecl136II GAGCTC 1 cut(s) 833
Eco24I GRGCYC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 833
Eco57I CTGAAG 2 cut(s) 423, 1004
Eco81I CCTNAGG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 833
EcoRII CCWGG 2 cut(s) 724, 1089
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 835
Esp3I CGTCTC 2 cut(s) 183, 219
FaeI CATG 9 cut(s) 67, 164, 423, 544, 569, 641, 791, 955, 1019
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 9 cut(s) 63, 160, 419, 540, 565, 637, 787, 951, 1015
Fnu4HI GCNGC 5 cut(s) 196, 199, 453, 456, 752
FokI GGATG 2 cut(s) 557, 1091
FriOI GRGCYC 1 cut(s) 835
Fsp4HI GCNGC 5 cut(s) 196, 199, 453, 456, 752
FspBI CTAG 2 cut(s) 731, 842
FspI TGCGCA 1 cut(s) 284
GlaI GCGC 1 cut(s) 284
GluI GCNGC 5 cut(s) 196, 199, 453, 456, 752
GsuI CTGGAG 1 cut(s) 762
HapII CCGG 2 cut(s) 245, 362
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 9 cut(s) 67, 164, 423, 544, 569, 641, 791, 955, 1019
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 6 cut(s) 146, 156, 677, 838, 880, 896
HpaII CCGG 2 cut(s) 245, 362
HphI GGTGA 3 cut(s) 597, 905, 985
Hpy166II GTNNAC 5 cut(s) 221, 373, 425, 605, 782
Hpy188I TCNGA 6 cut(s) 235, 583, 837, 941, 958, 1039
Hpy188III TCNNGA 5 cut(s) 180, 506, 643, 695, 788
Hpy8I GTNNAC 5 cut(s) 221, 373, 425, 605, 782
Hpy99I CGWCG 2 cut(s) 73, 214
HpyAV CCTTC 7 cut(s) 280, 460, 618, 785, 928, 970, 979
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 8 cut(s) 135, 458, 493, 523, 548, 710, 916, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 1061
HpyF3I CTNAG 9 cut(s) 142, 333, 338, 393, 673, 822, 876, 1022, 1038
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 9 cut(s) 67, 164, 423, 544, 569, 641, 791, 955, 1019
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
LmnI GCTCC 3 cut(s) 253, 530, 743
Lsp1109I GCAGC 4 cut(s) 207, 210, 442, 738
LweI GCATC 7 cut(s) 144, 256, 524, 535, 542, 719, 1036
MaeI CTAG 2 cut(s) 731, 842
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 893
MalI GATC 7 cut(s) 357, 510, 562, 624, 651, 776, 1010
MboI GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
MboII GAAGA 3 cut(s) 336, 416, 980
MflI RGATCY 2 cut(s) 508, 560
MhlI GDGCHC 1 cut(s) 835
MluCI AATT 5 cut(s) 107, 586, 597, 992, 1033
MlyI GAGTC 1 cut(s) 890
MnlI CCTC 7 cut(s) 219, 369, 624, 964, 1033, 1087, 1090
Mph1103I ATGCAT 1 cut(s) 495
MseI TTAA 2 cut(s) 129, 861
MspCI CTTAAG 1 cut(s) 860
MspI CCGG 2 cut(s) 245, 362
MspR9I CCNGG 2 cut(s) 726, 1091
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 2 cut(s) 726, 1091
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 192, 1061
NdeII GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
NlaIII CATG 9 cut(s) 67, 164, 423, 544, 569, 641, 791, 955, 1019
NmuCI GTSAC 1 cut(s) 893
NsbI TGCGCA 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 2 cut(s) 641, 955
PagI TCATGA 1 cut(s) 787
PaqCI CACCTGC 1 cut(s) 907
PciI ACATGT 1 cut(s) 951
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 5 cut(s) 146, 156, 677, 838, 880
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 1 cut(s) 208
PfoI TCCNGGA 2 cut(s) 724, 1089
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 5 cut(s) 197, 200, 454, 457, 753
PleI GAGTC 1 cut(s) 890
PpsI GAGTC 1 cut(s) 890
PscI ACATGT 1 cut(s) 951
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 835
Psp6I CCWGG 2 cut(s) 724, 1089
PspGI CCWGG 2 cut(s) 724, 1089
PspLI CGTACG 1 cut(s) 15
PspPI GGNCC 1 cut(s) 112
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 2 cut(s) 508, 560
PsyI GACNNNGTC 1 cut(s) 208
RsaI GTAC 6 cut(s) 17, 83, 220, 374, 691, 827
RsaNI GTAC 6 cut(s) 16, 82, 219, 373, 690, 826
SacI GAGCTC 1 cut(s) 835
SaqAI TTAA 2 cut(s) 129, 861
SatI GCNGC 5 cut(s) 196, 199, 453, 456, 752
Sau3AI GATC 7 cut(s) 355, 508, 560, 622, 649, 774, 1008
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 2 cut(s) 726, 1091
SduI GDGCHC 1 cut(s) 835
SfaNI GCATC 7 cut(s) 144, 256, 524, 535, 542, 719, 1036
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 3 cut(s) 695, 860, 1050
SmoI CTYRAG 3 cut(s) 695, 860, 1050
Sse9I AATT 5 cut(s) 107, 586, 597, 992, 1033
SsiI CCGC 2 cut(s) 36, 452
SspMI CTAG 2 cut(s) 731, 842
SstI GAGCTC 1 cut(s) 835
StyD4I CCNGG 2 cut(s) 724, 1089
TaiI ACGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 181, 347
TasI AATT 5 cut(s) 107, 586, 597, 992, 1033
TatI WGTACW 3 cut(s) 81, 372, 689
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 5 cut(s) 146, 156, 677, 838, 880
Tru1I TTAA 2 cut(s) 129, 861
Tru9I TTAA 2 cut(s) 129, 861
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 4 cut(s) 195, 198, 455, 751
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 6 cut(s) 17, 52, 408, 582, 776, 804
TspGWI ACGGA 3 cut(s) 238, 1058, 1091
Tth111I GACNNNGTC 1 cut(s) 208
Vha464I CTTAAG 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 992
XceI RCATGY 2 cut(s) 641, 955
XspI CTAG 2 cut(s) 731, 842
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.