Rh6DG421200

RNase H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001415
Physical Location & Seq
Forward (+)
60299617 .. 60302577
2961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG421200.1

Sequence Viewer

Length: 561 bp
ATGTCGTCGGTGTCTATAAGAGGCTCTTCGATATGTGATCCTCCTATTAGTGTGTACAAAGGGTACTCTATGCCTAAGAAGGATGAGCAATATAGGATCCAGCTAGCATCTTCAAAAGGCGAAACGTCTGACAAGACTGCAGCAGCCAAGAAGAGATCCCACCAAGAGGCTGAATCAGAAAATGTGGAAGTAATTGGTTCAACTTCTGTATCAGATACTCCTTCCAGAAAGCAGGCCAAGAAAAAGGCTGAAGTTGAAGTTCCCCCTTCTGGCCGTGGCTGTACTCTTCAATTTGATGGTGCATCAAAAGGAAATCCTGGAGTAGCTGGTGCGGGAGCTGTACTGCGAGCAGATGATGGAACCTTGATCTGTAAACTACGTGAAGGTCTGGGTGTTGCAACCAGTAATGCTGCTGAGTACCGAGCTGTTATGTTAGGGTTAAAATATGCGCTTAAAAAAGGTTTTTCCAGGATTTTTGTTCAAGGTGACTCCAAACTCGTCTGTATGCAGGTTGGTTATGTCGATATTTTCTGCTTCTCAAGCAGTTTGTGTCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cauli_VI DUF393 RVT_3

Protein Analysis

186

Amino Acids

19.74

Weight (kDa)

9.11

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 98 - 171 4.7e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 499
AciI CCGC 1 cut(s) 332
AclWI GGATC 4 cut(s) 32, 91, 104, 150
AcoI YGGCCR 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 5 cut(s) 56, 65, 283, 342, 419
AfiI CCNNNNNNNGG 2 cut(s) 166, 269
AgsI TTSAA 5 cut(s) 114, 201, 257, 290, 482
AjnI CCWGG 2 cut(s) 316, 467
AluBI AGCT 4 cut(s) 103, 326, 338, 425
AluI AGCT 4 cut(s) 103, 326, 338, 425
AlwI GGATC 4 cut(s) 32, 91, 104, 150
AoxI GGCC 2 cut(s) 234, 271
ApeKI GCWGC 3 cut(s) 140, 143, 410
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 497
AsuNHI GCTAGC 1 cut(s) 103
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BamHI GGATCC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 152, 155, 397
BccI CCATC 2 cut(s) 290, 350
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 318, 469
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 138
BfuAI ACCTGC 1 cut(s) 499
BisI GCNGC 3 cut(s) 141, 144, 411
BlsI GCNGC 3 cut(s) 142, 145, 412
Bme1390I CCNGG 2 cut(s) 318, 469
BmiI GGNNCC 2 cut(s) 98, 361
BmrFI CCNGG 2 cut(s) 318, 469
BmsI GCATC 2 cut(s) 116, 311
BmtI GCTAGC 1 cut(s) 107
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 523
BsaAI YACGTR 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 269
Bse1I ACTGG 1 cut(s) 402
BseBI CCWGG 2 cut(s) 318, 469
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 2 cut(s) 166, 269
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 402
BseXI GCAGC 3 cut(s) 152, 155, 397
BshFI GGCC 2 cut(s) 236, 273
BslI CCNNNNNNNGG 2 cut(s) 166, 269
BsnI GGCC 2 cut(s) 236, 273
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 4 cut(s) 37, 96, 155, 366
BspACI CCGC 1 cut(s) 332
BspANI GGCC 2 cut(s) 236, 273
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 2 cut(s) 98, 361
BspMAI CTGCAG 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 499
BspOI GCTAGC 1 cut(s) 107
BspPI GGATC 4 cut(s) 32, 91, 104, 150
BspQI GCTCTTC 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 4 cut(s) 37, 96, 155, 366
Bst2UI CCWGG 2 cut(s) 318, 469
Bst6I CTCTTC 3 cut(s) 31, 146, 291
BstAUI TGTACA 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 105, 234, 348
BstDEI CTNAG 2 cut(s) 75, 414
BstDSI CCRYGG 1 cut(s) 274
BstF5I GGATG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 4 cut(s) 40, 99, 158, 369
BstMBI GATC 4 cut(s) 37, 96, 155, 366
BstMWI GCNNNNNNNGC 1 cut(s) 540
BstNI CCWGG 2 cut(s) 318, 469
BstSCI CCNGG 2 cut(s) 316, 467
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 152, 155, 397
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 2 cut(s) 96, 155
BstYI RGATCY 2 cut(s) 96, 155
BsuRI GGCC 2 cut(s) 236, 273
BtgI CCRYGG 1 cut(s) 274
BtsCI GGATG 1 cut(s) 88
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 105, 234, 348
CfoI GCGC 1 cut(s) 451
Csp6I GTAC 5 cut(s) 55, 64, 282, 341, 418
CviQI GTAC 5 cut(s) 55, 64, 282, 341, 418
DdeI CTNAG 2 cut(s) 75, 414
DpnI GATC 4 cut(s) 39, 98, 157, 368
DpnII GATC 4 cut(s) 37, 96, 155, 366
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 31, 146, 291
EarI CTCTTC 3 cut(s) 31, 146, 291
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 2 cut(s) 316, 467
FaiI YATR 8 cut(s) 17, 34, 71, 93, 431, 447, 506, 519
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 141, 144, 411
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 3 cut(s) 141, 144, 411
FspBI CTAG 1 cut(s) 104
GlaI GCGC 1 cut(s) 450
GluI GCNGC 3 cut(s) 141, 144, 411
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 236, 273
HhaI GCGC 1 cut(s) 451
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HinfI GANTC 2 cut(s) 173, 488
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 2 cut(s) 55, 374
Hpy188I TCNGA 4 cut(s) 130, 178, 214, 560
Hpy188III TCNNGA 1 cut(s) 225
Hpy8I GTNNAC 2 cut(s) 55, 374
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 73, 231, 276, 377
HpyCH4IV ACGT 2 cut(s) 125, 379
HpyCH4V TGCA 4 cut(s) 140, 302, 398, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 540
HpyF3I CTNAG 2 cut(s) 75, 414
HpySE526I ACGT 2 cut(s) 125, 379
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 4 cut(s) 37, 96, 155, 366
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 3 cut(s) 152, 155, 397
LweI GCATC 2 cut(s) 116, 311
MaeI CTAG 1 cut(s) 104
MaeII ACGT 2 cut(s) 125, 379
MaeIII GTNAC 1 cut(s) 485
MalI GATC 4 cut(s) 39, 98, 157, 368
MboI GATC 4 cut(s) 37, 96, 155, 366
MboII GAAGA 5 cut(s) 18, 102, 163, 278, 545
MflI RGATCY 2 cut(s) 96, 155
MluCI AATT 2 cut(s) 192, 290
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 3 cut(s) 14, 51, 160
MseI TTAA 2 cut(s) 440, 453
MspR9I CCNGG 2 cut(s) 318, 469
MvaI CCWGG 2 cut(s) 318, 469
MwoI GCNNNNNNNGC 1 cut(s) 540
NdeII GATC 4 cut(s) 37, 96, 155, 366
NheI GCTAGC 1 cut(s) 103
NlaIV GGNNCC 2 cut(s) 98, 361
NmuCI GTSAC 1 cut(s) 485
PciSI GCTCTTC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 173
PfoI TCCNGGA 2 cut(s) 316, 467
PkrI GCNGC 3 cut(s) 142, 145, 412
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
Ppu21I YACGTR 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 316, 467
PspGI CCWGG 2 cut(s) 316, 467
PspN4I GGNNCC 2 cut(s) 98, 361
PstI CTGCAG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 96, 155
RsaI GTAC 5 cut(s) 56, 65, 283, 342, 419
RsaNI GTAC 5 cut(s) 55, 64, 282, 341, 418
SapI GCTCTTC 1 cut(s) 31
SaqAI TTAA 2 cut(s) 440, 453
SatI GCNGC 3 cut(s) 141, 144, 411
Sau3AI GATC 4 cut(s) 37, 96, 155, 366
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 318, 469
SfaNI GCATC 2 cut(s) 116, 311
SfcI CTRYAG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 2 cut(s) 192, 290
SsiI CCGC 1 cut(s) 332
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 316, 467
TaiI ACGT 2 cut(s) 128, 382
TaqI TCGA 2 cut(s) 29, 522
TasI AATT 2 cut(s) 192, 290
TatI WGTACW 3 cut(s) 54, 281, 340
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 2 cut(s) 440, 453
Tru9I TTAA 2 cut(s) 440, 453
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 3 cut(s) 140, 143, 410
Tsp45I GTSAC 1 cut(s) 485
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.